Methods for enhancing microbial production of specific length fatty alcohols in the presence of methanol

ABSTRACT

The invention provides non-naturally occurring microbial organisms having a formaldehyde fixation pathway, a formate assimilation pathway, and/or a methanol metabolic pathway in combination with a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length or isopropanol. The microbial organisms provided advantageously enhance the production of substrates and/or pathway intermediates for the production of chain length specific fatty alcohols, fatty aldehydes, fatty acids or isopropanol. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde, a fatty acid or isopropanol.

CROSS-REFERENCE TO RELAYED APPLICATIONS

This application claims the benefit of priority of U.S. Provisional Application Ser. No. 61/945,003, filed Feb. 26, 2014, 61/911,374, filed Dec. 3, 2013, and 61/908,652, filed Nov. 25, 2013, the entire contents of which are each incorporated herein by reference.

BACKGROUND OF THE INVENTION

The present invention relates generally to biosynthetic processes, and more specifically to organisms having specific length fatty alcohol, fatty aldehyde or fatty acid biosynthetic capacity or having isopropanol biosynthetic capacity.

Primary alcohols are a product class of compounds having a variety of industrial applications which include a variety of biofuels and specialty chemicals. Primary alcohols also can be used to make a large number of additional industrial products including polymers and surfactants. For example, higher primary alcohols, also known as fatty alcohols (C₄-C₂₄) and their ethoxylates are used as surfactants in many consumer detergents, cleaning products and personal care products worldwide such as laundry powders and liquids, dishwashing liquid and hard surface cleaners. They are also used in the manufacture of a variety of industrial chemicals and in lubricating oil additives. Specific length fatty alcohols, such as octanol and hexanol, have useful organoleptic properties and have long been employed as fragrance and flavor materials. Smaller chain length C₄-C₈ alcohols (e.g., butanol) are used as chemical intermediates for production of derivatives such as acrylates used in paints, coatings, and adhesives applications.

Fatty alcohols are currently produced from, for example, hydrogenation of fatty acids, hydroformylation of terminal olefins, partial oxidation of n-paraffins and the Al-catalyzed polymerization of ethylene. Unfortunately, it is not commercially viable to produce fatty alcohols directly from the oxidation of petroleum-based linear hydrocarbons (n-paraffins). This impracticality is because the oxidation of n-paraffins produces primarily secondary alcohols, tertiary alcohols or ketones, or a mixture of these compounds, but does not produce high yields of fatty alcohols. Additionally, currently known methods for producing fatty alcohols suffer from the disadvantage that they are restricted to feedstock which is relatively expensive, notably ethylene, which is produced via the thermal cracking of petroleum. In addition, current methods require several steps, and several catalyst types.

Fatty alcohol production by microorganisms involves fatty acid synthesis followed by acyl-reduction steps. The universal fatty acid biosynthesis pathway found in most cells has been investigated for production of fatty alcohols and other fatty acid derivatives. There is currently a great deal of improvement that can be achieved to provide more efficient biosynthesis pathways for fatty alcohol production with significantly higher theoretical product and energy yields.

Isopropanol (IPA) is a colorless, flammable liquid that mixes completely with most solvents, including water. The largest use for IPA is as a solvent, including its well known yet small use as “rubbing alcohol,” which is a mixture of IPA and water. As a solvent, IPA is found in many everyday products such as paints, lacquers, thinners, inks, adhesives, general-purpose cleaners, disinfectants, cosmetics, toiletries, de-icers, and pharmaceuticals. Low-grade IPA is also used in motor oils. The second largest use is as a chemical intermediate for the production of isopropylamines, isopropylethers, and isopropyl esters. Isopropanol can potentially be dehydrated to form propylene, a polymer precursor with an annual market of more than 2 million metric tons.

Current global production capacity of IPA is approximately 6 B lb/yr, with approximately 74% of global IPA capacity concentrated in the US, Europe, and Japan. Isopropanol is manufactured by two petrochemical routes. The predominant process entails the hydration of propylene either with or without sulfuric acid catalysis. Secondarily, IPA is produced via hydrogenation of acetone, which is a by-product formed in the production of phenol and propylene oxide. High-priced propylene is currently driving costs up and margins down throughout the chemical industry motivating the need for an expanded range of low cost feedstocks.

Thus, there exists a need for alternative means for effectively producing commercial quantities of fatty alcohols, isopropanol and related compounds. The present invention satisfies this need and provides related advantages as well.

SUMMARY OF INVENTION

The invention provides non-naturally occurring microbial organisms containing a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway. For production of a fatty alcohol, fatty aldehyde, or fatty acid, in some embodiments, the non-naturally occurring microbial organism of the invention has: a formaldehyde fixation pathway, a formate assimilation pathway, and/or a methanol metabolic pathway; and a malonyl-CoA independent fatty acyl-CoA elongation (MI-FAE) cycle and/or a malonyl-CoA dependent fatty acyl-CoA elongation (MD-FAE) cycle in combination with a termination pathway, as depicted in FIGS. 1, 2, 7, 8 and 10. Alternatively, in some embodiments, the non-naturally occurring microbial organism of the invention has: a formaldehyde fixation pathway, a formate assimilation pathway, and/or a methanol metabolic pathway; and a fatty acyl-ACP elongation (FAACPE) cycle in combination with a termination pathway, as depicted in FIGS. 1, 10 and 12.

For production of isopropanol, in some embodiments, the non-naturally occurring microbial organism of the invention has: a formaldehyde fixation pathway, a formate assimilation pathway, and/or a methanol metabolic pathway; and an isopropanol pathway, as depicted in FIGS. 1, 10 and 11.

In one aspect, the formaldehyde fixation pathway, formate assimilation pathway, and/or a methanol metabolic pathway present in the microbial organisms of the invention enhances the availability of substrates and/or pathway intermediates, such as acetyl-CoA and malonyl-CoA, and/or reducing equivalents, which can be utilized for fatty alcohol, fatty aldehyde, fatty acid, or isopropanol production through one or more fatty alcohol, fatty aldehyde, fatty acid, or isopropanol pathways of the invention. For example, in some embodiments, a non-naturally occurring microbial organism of the invention that includes a methanol metabolic pathway can enhance the availability of reducing equivalents in the presence of methanol and/or convert methanol to formaldehyde, a substrate for the formaldehyde fixation pathway. Likewise, a non-naturally occurring microbial organism of the invention having a formate assimilation pathway can reutilize formate to generate substrates and pathway intermediates such as formaldehyde, pyruvate and/or acetyl-CoA. Such substrates, intermediates and reducing equivalents can be used to increase the yield of a fatty alcohol, a fatty aldehyde, a fatty acid, or isopropanol produced by the microbial organism.

In some embodiments, the microbial organisms of the invention advantageously enhance the production of substrates and/or pathway intermediates for the production of a chain length specific fatty alcohol, fatty aldehyde, fatty acid. Accordingly, some embodiments, one or more enzymes of the formaldehyde fixation pathway, formate assimilation pathway, methanol metabolic pathway, MI-FAE cycle, MD-FAE cycle, FAACPE cycle or termination pathway is encoded by at least one exogenous nucleic acid and is expressed in a sufficient amount to produce a fatty alcohol, fatty aldehyde or fatty acid of Formula (I):

wherein R₁ is C₁₋₂₄ linear alkyl; R₂ is CH₂OH, CHO, or COOH; R₃ is H, OH, or oxo (═O); and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four. In order to be able to produce a chain length specific compound, the enzymes of the MI-FAE cycle, the MD-FAE cycle, the FAACPE cycle and/or the termination pathway are selective for a particular substrate. Accordingly, in some embodiments, the substrate of each of the enzymes of the MI-FAE cycle, the MD-FAE cycle and/or the termination pathway are independently selected from a compound of Formula (II), malonyl-CoA, propionyl-CoA or acetyl-CoA:

wherein R₁ is C₁₋₂₄ linear alkyl; R₃ is H, OH, or oxo (═O); R₄ is S-CoA, ACP, OH or H; and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four, wherein said one or more enzymes of the MI-FAE cycle are each selective for a compound of Formula (II) having a number of carbon atoms at R₁ that is no greater than the number of carbon atoms at R₁ of said fatty alcohol, fatty aldehyde or fatty acid of Formula (I), wherein said one or more enzymes of the MD-FAE cycle are each selective for a compound of Formula (II) having a number of carbon atoms at R₁ that is no greater than the number of carbon atoms at R₁ of said fatty alcohol, fatty aldehyde or fatty acid of Formula (I), and wherein said one or more enzymes of the termination pathway are each selective for a compound of Formula (II) having a number of carbon atoms at R₁ that is no less than the number of carbon atoms at R₁ of said fatty alcohol, fatty aldehyde or fatty acid of Formula (I). Alternatively, in some embodiments, the substrate of each of the enzymes of the FAACPE cycle and/or the termination pathway are independently selected from a compound of Formula (II) or malonyl-ACP:

wherein R₁ is C₁₋₂₄ linear alkyl; R₃ is H, OH, or oxo (═O); R₄ is S-CoA, ACP, OH or H; and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four, wherein the one or more enzymes of the FAACPE cycle are each selective for a compound of Formula (II) having a number of carbon atoms at R₁ that is no greater than the number of carbon atoms at R₁ of said compound of Formula (I), and wherein the one or more enzymes of the termination pathway are each selective for a compound of Formula (II) having a number of carbon atoms at R₁ that is no less than the number of carbon atoms at R₁ of said compound of Formula (I).

In some embodiments, the invention provides a non-naturally occurring microbial organism containing a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway further having an acetyl-CoA pathway, a methanol oxidation pathway, a hydrogenase and/or a carbon monoxide dehydrogenase. Accordingly, in some embodiments, the invention provides a non-naturally occurring microbial organism having a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway, wherein the microbial organism further includes an acetyl-CoA pathway and at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme expressed in a sufficient amount to produce or enhance carbon flux through acetyl-CoA, wherein the acetyl-CoA pathway includes a pathway shown in FIG. 1, 3, 4, 5 or 6. In some embodiments, the invention provides a non-naturally occurring microbial organism having a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway, wherein the microbial organism further includes a methanol oxidation pathway enzyme expressed in a sufficient amount to produce formaldehyde in the presence of methanol. An exemplary methanol oxidation pathway enzyme is a methanol dehydrognease as depicted in FIG. 1, Step A. In some embodiments, the invention provides a non-naturally occurring microbial organism having a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway, wherein the microbial organism further includes a hydrogenase and/or a carbon monoxide dehydrogenase for generating reducing equivalents as depicted in FIG. 10.

In some embodiments, the invention provides a non-naturally occurring microbial organism having a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organism has one or more gene disruptions, wherein the one or more gene disruptions occur in endogenous genes encoding proteins or enzymes involved in: native production of ethanol, glycerol, pyruvate, acetate, formate, lactate, CO₂, fatty acids, or malonyl-CoA by said microbial organism; transfer of pathway intermediates to cellular compartments other than the cytosol; or native degradation of a MI-FAE cycle intermediate, MD-FAE cycle intermediate, FAACPE cycle intermediate or a termination pathway intermediate by the microbial organism, the one or more gene disruptions confer increased production of a fatty alcohol, fatty aldehyde or fatty acid in the microbial organism.

In some embodiments, the invention provides a non-naturally occurring microbial organism having a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein one or more enzymes of the MI-FAE cycle, MD-FAE cycle, FAACPE cycle or the termination pathway preferentially react with an NADH cofactor or have reduced preference for reacting with an NAD(P)H cofactor.

In some embodiments, the invention provides a non-naturally occurring microbial organism having a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organism has one or more gene disruptions in genes encoding proteins or enzymes that result in an increased ratio of NAD(P)H to NAD(P) present in the cytosol of the microbial organism following the disruptions.

In some embodiments, the non-naturally occurring microbial organism of the invention is Crabtree positive and is in culture medium comprising excess glucose. In such conditions, as described herein, the microbial organism can result in increasing the ratio of NAD(P)H to NAD(P) present in the cytosol of the microbial organism.

In some embodiments, the invention provides a non-naturally occurring microbial organism having a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organism has at least one exogenous nucleic acid encoding an extracellular transporter or an extracellular transport system for a fatty alcohol, fatty aldehyde or fatty acid of the invention.

In some embodiments, the invention provides a non-naturally occurring microbial organism having a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organism one or more endogenous enzymes involved in: native production of ethanol, glycerol, pyruvate, acetate, formate, lactate, CO₂, fatty acids, or malonyl-CoA by said microbial organism; transfer of pathway intermediates to cellular compartments other than the cytosol; or native degradation of a MI-FAE cycle intermediate, a MD-FAE cycle intermediate, FAACPE cycle intermediate or a termination pathway intermediate by said microbial organism, has attenuated enzyme activity or expression levels.

In some embodiments, the invention provides a non-naturally occurring microbial organism having a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organism has attenuated enzyme activity or expression levels for one or more endogenous enzymes involved in the oxidation of NAD(P)H or NADH.

In some embodiments, the invention provides a non-naturally occurring microbial organism having a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organism further includes attenuation of one or more endogenous enzymes, which enhances carbon flux through acetyl-CoA, or a gene disruption of one or more endogenous nucleic acids encoding such enzymes. For example, in some aspects, the endogenous enzyme can be selected from DHA kinase, methanol oxidase, PQQ-dependent methanol dehydrogenase, DHA synthase or any combination thereof.

The invention further provides non-naturally occurring microbial organisms that have elevated or enhanced synthesis or yields of acetyl-CoA (e.g. intracellular) or biosynthetic products such as a fatty alcohol, fatty aldehyde, fatty acid or isopropanol and methods of using those non-naturally occurring organisms to produce such biosynthetic products. The enhanced synthesis of intracellular acetyl-CoA enables enhanced production of a fatty alcohol, fatty aldehyde, fatty acid or isopropanol from which acetyl-CoA is an intermediate and further, may have been rate limiting.

In some embodiments, the invention provides a non-naturally occurring microbial organism having a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organism further includes attenuation of one or more endogenous enzymes of a competing formaldehyde assimilation or dissimilation pathway or a gene disruption of one or more endogenous nucleic acids encoding enzymes of a competing formaldehyde assimilation or dissimilation pathway. Examples of these endogenous enzymes are described herein.

The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde, a fatty acid or isopropanol by culturing a non-naturally occurring microbial organism containing a fatty alcohol, fatty aldehyde, fatty acid or isopropnaol pathway as described herein under conditions and for a sufficient period of time to produce a fatty alcohol, fatty aldehyde, fatty acid or isopropanol.

The invention still further provides a bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol produced by a microbial organism of the invention, culture medium having the bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol of the invention, compositions having the bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol of the invention, a biobased product comprising the bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol of the invention, and a process for producing a bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol of the invention.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows exemplary metabolic pathways enabling the conversion of CO2, formate, formaldehyde (Fald), methanol (MeOH), glycerol, xylose (XYL) and glucose (GLC) to acetyl-CoA (ACCOA) and exemplary endogenous enzyme targets for optional attenuation or disruption. The exemplary pathways and endogenous enzyme targets can be combined with the cycles and pathways depicted herein that utilize ACCOA, such as those depicted in FIGS. 1, 11 and 12. The enzyme targets are indicated by arrows having “X” markings. The endogenous enzyme targets include DHA kinase, methanol oxidase (AOX), PQQ-dependent methanol dehydrogenase (PQQ) and/or DHA synthase. The enzymatic transformations shown are carried out by the following enzymes: A) methanol dehydrogenase, B) 3-hexulose-6-phosphate synthase, C) 6-phospho-3-hexuloisomerase, D) dihydroxyacetone synthase, E) formate reductase, F) formate ligase, formate transferase, or formate synthetase, G) formyl-CoA reductase, H) formyltetrahydrofolate synthetase, I) methenyltetrahydrofolate cyclohydrolase, methylenetetrahydrofolate dehydrogenase, K) spontaneous or formaldehyde-forming enzyme, L) glycine cleavage system, M) serine hydroxymethyltransferase, N) serine deaminase, O) methylenetetrahydrofolate reductase, P) acetyl-CoA synthase, Q) pyruvate formate lyase, R) pyruvate dehydrogenase, pyruvate ferredoxin oxidoreductase, or pyruvate:NADP+ oxidoreductase, S) formate dehydrogenase, T) fructose-6-phosphate phosphoketolase, U) xylulose-5-phosphate phosphoketolase, V) phosphotransacetylase, W) acetate kinase, X) acetyl-coa transferase, synthetase, or ligase, Y) lower glycolysis including glyceraldehyde-3-phosphate dehydrogenase, Z) fructose-6-phosphate aldolase. See abbreviation list below for compound names.

FIG. 2 shows an exemplary MI-FAE cycle and/or MD-FAE cycle in combination with termination pathways for production of fatty alcohols, aldehydes, or acids from the acyl-CoA intermediate of the MI-FAE cycle or MD-FAE cycle. Enzymes are: A. Thiolase; B. 3-Oxoacyl-CoA reductase; C. 3-Hydroxyacyl-CoA dehydratase; D. Enoyl-CoA reductase; E. Acyl-CoA reductase (aldehyde forming); F. Alcohol dehydrogenase; G. Acyl-CoA reductase (alcohol forming); H. acyl-CoA hydrolase, transferase or synthase; J. Acyl-ACP reductase; K. Acyl-CoA:ACP acyltransferase; L. Thioesterase; N. Aldehyde dehydrogenase (acid forming) or carboxylic acid reductase; O. Elongase; and P. acyl-ACP reductase (alcohol forming).

FIG. 3 shows exemplary pathways for production of cytosolic acetyl-CoA from pyruvate or threonine. Enzymes are: A. pyruvate oxidase (acetate-forming); B. acetyl-CoA synthetase, ligase or transferase; C. acetate kinase; D. phosphotransacetylase; E. pyruvate decarboxylase; F. acetaldehyde dehydrogenase; G. pyruvate oxidase (acetyl-phosphate forming); H. pyruvate dehydrogenase, pyruvate:ferredoxin oxidoreductase, pyruvate:NAD(P)H oxidoreductase or pyruvate formate lyase; I. acetaldehyde dehydrogenase (acylating); and J. threonine aldolase.

FIG. 4 shows exemplary pathways for production of acetyl-CoA from phosphoenolpyruvate (PEP). Enzymes are: A. PEP carboxylase or PEP carboxykinase; B. oxaloacetate decarboxylase; C. malonate semialdehyde dehydrogenase (acetylating); D. acetyl-CoA carboxylase or malonyl-CoA decarboxylase; F. oxaloacetate dehydrogenase or oxaloacetate oxidoreductase; G. malonate semialdehyde dehydrogenase (acylating); H. pyruvate carboxylase; J. malonate semialdehyde dehydrogenase; K. malonyl-CoA synthetase or transferase; L. malic enzyme; M. malate dehydrogenase or oxidoreductase; and N. pyruvate kinase or PEP phosphatase.

FIG. 5 shows exemplary pathways for production of cytosolic acetyl-CoA from mitochondrial acetyl-CoA using citrate and malate transporters. Enzymes are: A. citrate synthase; B. citrate transporter; C. citrate/malate transporter; D. ATP citrate lyase; E. citrate lyase; F. acetyl-CoA synthetase or transferase; H. cytosolic malate dehydrogenase; I. malate transporter; J. mitochondrial malate dehydrogenase; K. acetate kinase; and L. phosphotransacetylase.

FIG. 6 shows exemplary pathways for production of cytosolic acetyl-CoA from mitochondrial acetyl-CoA using citrate and oxaloacetate transporters. Enzymes are: A. citrate synthase; B. citrate transporter; C. citrate/oxaloacetate transporter; D. ATP citrate lyase; E. citrate lyase; F. acetyl-CoA synthetase or transferase; G) oxaloacetate transporter; K) acetate kinase; and L) phosphotransacetylase.

FIG. 7 shows an exemplary MI-FAE cycle and/or MD-FAE cycle for elongating the linear alkyl of Enzymes are: A. Thiolase; B. 3-Ketoacyl-CoA reductase; C. 3-Hydroxyacyl-CoA dehydratase; D. Enoyl-CoA reductase; and E. Elongase.

FIG. 8 shows an exemplary termination cycle for generating a fatty alcohol, fatty aldehyde or fatty acid from any of the MI-FAE cycle intermediates or MD-FAE cycle intermediates of FIG. 7. Enzymes are: E. MI-FAE/MD-FAE intermediate-CoA reductase (aldehyde forming); F. Alcohol dehydrogenase; G. MI-FAE/MD-FAE intermediate-CoA reductase (alcohol forming); H. MI-FAE/MD-FAE intermediate-CoA hydrolase, transferase or synthase; J. MI-FAE/MD-FAE intermediate-ACP reductase; K. MI-FAE/MD-FAE intermediate-CoA:ACP acyltransferase; L. Thioesterase; N. Aldehyde dehydrogenase (acid forming) or carboxylic acid reductase; and P. acyl-ACP reductase (alcohol forming). R1 is C1-24 linear alkyl; R₃ is H, OH, or oxo (═O) and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four.

FIG. 9 shows exemplary compounds that can be produced from the four MI-FAE or MD-FAE cycle intermediates using the cycles depicted in FIG. 6 and the termination pathways depicted in FIG. 7. R is C₁₋₂₄ linear alkyl.

FIG. 10 shows exemplary metabolic pathways that provide the extraction of reducing equivalents from methanol, hydrogen, or carbon monoxide. Enzymes are: A) methanol methyltransferase, B) methylenetetrahydrofolate reductase, C) methylenetetrahydrofolate dehydrogenase, D) methenyltetrahydrofolate cyclohydrolase, E) formyltetrahydrofolate deformylase, F) formyltetrahydrofolate synthetase, G) formate hydrogen lyase, H) hydrogenase, I) formate dehydrogenase, J) methanol dehydrogenase, K) spontaneous or formaldehyde activating enzyme, L) formaldehyde dehydrogenase, M) spontaneous or S-(hydroxymethyl)glutathione synthase, N) Glutathione-Dependent Formaldehyde Dehydrogenase, O)S-formylglutathione hydrolase, P) carbon monoxide dehydrogenase. See abbreviation list below for compound names.

FIG. 11 shows exemplary metabolic pathways enabling the conversion of acetyl-CoA to isopropanol. Enzymes are: T) acetyl-CoA carboxylase, U) acetoacetyl-CoA synthase, V) acetyl-CoA:acetyl-CoA acyltransferase, W) acetoacetyl-CoA hydrolase, acetoacetyl-CoA transferase, acetoacetyl-CoA ligase, phosphotransacetoacetylase/acetoacetate kinase, X) acetoacetate decarboxylase, Y) acetone reductase (or isopropanol dehydrogenase). See abbreviation list below for compound names.

FIG. 12 shows an exemplary β-ketoacyl-ACP pathway, a FAACPE cycle in combination with termination pathways for production of fatty alcohols, aldehydes, or acids from the acyl-ACP intermediate of the FAACPE cycle. Enzymes are: A) Acetyl-CoA carboxylase, B) Malonyl-CoA ACP transacylase, C) Acetoacetyl-ACP synthase, D) β-Ketoacyl-ACP synthase, E) β-Ketoacyl-ACP reductase, F) β-Hydroxyacyl-ACP reductase, G) Enoyl ACP-reductase, H) β-Ketoacyl-ACP synthase, I) Thioesterase, J) Fatty acyl-ACP reductase, K) Acyl-CoA synthase, L) Acyl-CoA reductase, M) Fatty aldehyde reductase, N) Fatty alcohol forming acyl-CoA reductase (FAR), O) Carboxylic acid reductase (CAR), and P) acyl-ACP reductase (alcohol forming).

FIG. 13 depicts the production of 1,3-butanediol (FIG. 13A) or ethanol (FIG. 13B) in S. cerevisiae transformed with plasmids comprising genes encoding various MI-FAE cycle and termination pathway enzymes, either with or without pflAV or PDH bypass, as provided in Example XIII.

FIG. 14 depicts the production of pyruvic acid (FIG. 14A), succinic acid (FIG. 14B), acetic acid (FIG. 14C) or glucose (FIG. 14D) in S. cerevisiae transformed with plasmids comprising genes encoding various MI-FAE cycle and termination pathway enzymes, either with or without pflAV or PDH bypass, as provided in Example XIII.

FIG. 15 depicts the production of 1,3-butanediol in S. cerevisiae transformed with plasmids comprising genes encoding various MI-FAE cycle and termination pathway enzymes, either with or without pflAV or PDH bypass, as provided in Example XIII.

FIG. 16 depicts the estimated specific activity of five thiolases for acetyl-CoA condensation activity in E. coli as provided in Example XIV.

FIGS. 17A and 17B depict the estimated specific activity of two thiolases (1491 and 560) cloned in dual promoter yeast vectors with 1495 (a 3-hydroxybutyryl-CoA dehydrogenase) for acetyl-CoA condensation activity in E. coli as provided in Example XIV.

FIG. 18 depicts the time course of fluorescence detection of oxidation of NADH, which is used to measure the metabolism of acetoacetyl-CoA to 3-hydroxybutyryl-CoA by 3-hydroxybutyryl-CoA dehydrogenase, as provided in Example XIV. Acetoacetyl-CoA is metabolized to 3-hydroxybutyryl-CoA by 3-hydroxybutyryl-CoA dehydrogenase. The reaction requires oxidation of NADH, which can be monitored by fluorescence at an excitation wavelength at 340 nm and an emission at 460 nm. The oxidized form, NAD+, does not fluoresce. 1495, the Hbd from Clostridium beijerinckii, was assayed in the dual promoter yeast vectors that contained either 1491 (vector id=pY3Hd17) or 560 (vector id=pY3Hd16).

FIG. 19 depicts levels of NAD(P)H oxidation in the presence of 1 or 5 ug/ml NADH or 1 or 5 ug/ml NADPH, and shows that the Hbd prefers NADH over NADPH, as provided in Example XIV.

FIG. 20 depicts the activity data for crude lysates of an aldehyde reductase that converts 3-hydroxybutyryl-CoA to 3-hydroxybutyraldehyde and requires NAD(P)H oxidation, which can be used to monitor enzyme activity, as provided in Example XIV. The Ald from Lactobacillus brevis (Gene ID 707) was cloned in a dual vector that contained the alcohol dehydrogenase from Clostridium saccharoperbutylacetonicum (Gene ID 28). These two enzymes were cloned in another dual promoter yeast vector containing a Leu marker. A 707 lysate from E. coli was used as a standard.

FIG. 21 depicts the evaluation of ADH (Gene 28) in the dual promoter vector with ALD (Gene 707) with butyraldehyde, a surrogate substrate for 3-hydroxybutyraldehyde. 1,3-BDO is formed by an alcohol dehydrogenase (Adh), which reduces 3-hydroxybutyraldehyde in the presence of NAD(P)H, and the oxidation of NAD(P)H is used to monitor the reaction.

FIG. 22 depicts exemplary pathways for production of propionyl-CoA. Enzymes are: A) PEP carboxykinase, B) PEP carboxylase, C) Pyruvate kinase, D) Pyruvate carboxylase, E) Malate dehydrogenase, F) Fumarase, G) Fumarate reductase, H) Succinyl-CoA synthetase, I) Succinyl-CoA:3-ketoacid-CoA transferase, J) Methylmalonyl-CoA mutase, K) Methyl-malonyl-CoA epimerase, L) Methylmalonyl-CoA decarboxylase. See abbreviation list below for compound names.

DETAILED DESCRIPTION OF THE INVENTION

The present invention is directed to metabolic and biosynthetic processes and microbial organisms capable of producing fatty alcohols, fatty aldehydes, fatty acids or isopropanol. The invention disclosed herein is based, at least in part, on non-naturally occurring microbial organisms capable of synthesizing fatty alcohols, fatty aldehydes, or fatty acids using a formaldehyde fixation pathway, a formate assimilation pathway and/or a methoanol metabolic pathway with a malonyl-CoA-independent fatty acid elongation (MI-FAE) cycle and/or malonyl-CoA dependent fatty acid elongation cycle (MD-FAE) cycle in combination with a termination pathway, or in some embodiments a fatty acyl-ACP elongation (FAACPE) cycle in combination with a termination pathway. The invention disclosed herein is also based, at least in part, on non-naturally occurring microbial organisms capable of synthesizing isopropanol using a formaldehyde fixation pathway, a formate assimilation pathway and/or a methoanol metabolic pathway in combination with an isopropanol pathway. Additionally, in some embodiments, the non-naturally occurring microbial organisms can further include a methanol oxidation pathway, an acetyl-CoA pathway, a hydrogenase and/or a carbon monoxide dehydrogenase.

The following is a list of abbreviations and their corresponding compound or composition names. These abbreviations, which are used throughout the disclosure and the figures. It is understood that one of ordinary skill in the art can readily identify these compounds/compositions by such nomenclature. MeOH or MEOH=methanol; Fald=formaldehyde; GLC=glucose; G6P=glucose-6-phosphate; H6P=hexulose-6-phosphate; F6P=fructose-6-phosphate; FDP=fructose diphosphate or fructose-1,6-diphosphate; DHA=dihydroxyacetone; DHAP=dihydroxyacetone phosphate; G3P=and glyceraldehyde-3-phosphate; PYR=pyruvate; ACTP=acetyl-phosphate; ACCOA=acetyl-CoA; AACOA=acetoacetyl-CoA; MALCOA=malonyl-CoA; FTHF=formyltetrahydrofolate; THF=tetrahydrofolate; E4P=erythrose-4-phosphate: Xu5P=xyulose-5-phosphate; Ru5P=ribulose-5-phosphate; S7P=sedoheptulose-7-phosphate: R5P=ribose-5-phosphate; TCA=tricarboxylic acid; PEP=Phosphoenolpyruvate; OAA=Oxaloacetate; MAL=malate; FUM=Fumarate; SUCC=Succinate; SUCCOA=Succinyl-CoA; (R)-MMCOA=R-Methylmalonyl-CoA; (S)-MMCOA=S-Methylmalonyl-CoA; PPCOA=Propionyl-CoA.

It is also understood that association of multiple steps in a pathway can be indicated by linking their step identifiers with or without spaces or punctuation; for example, the following are equivalent to describe the 4-step pathway comprising Step W, Step X, Step Y and Step Z: steps WXYZ or W,X,Y,Z or W;X;Y;Z or W-X-Y-Z. One of ordinary skill can readily distinguish a single step designator of “AA” or “AB” or “AD” from a multiple step pathway description based on context and use in the description and figures herein.

As used herein, the term “non-naturally occurring” when used in reference to a microbial organism or microorganism of the invention is intended to mean that the microbial organism has at least one genetic alteration not normally found in a naturally occurring strain of the referenced species, including wild-type strains of the referenced species. Genetic alterations include, for example, modifications introducing expressible nucleic acids encoding metabolic polypeptides, other nucleic acid additions, nucleic acid deletions and/or other functional disruption of the microbial organism's genetic material. Such modifications include, for example, coding regions and functional fragments thereof, for heterologous, homologous or both heterologous and homologous polypeptides for the referenced species. Additional modifications include, for example, non-coding regulatory regions in which the modifications alter expression of a gene or operon. Exemplary metabolic polypeptides include enzymes or proteins within a fatty alcohol, fatty aldehyde or fatty alcohol biosynthetic pathway.

A metabolic modification refers to a biochemical reaction that is altered from its naturally occurring state. Therefore, non-naturally occurring microorganisms can have genetic modifications to nucleic acids encoding metabolic polypeptides, or functional fragments thereof. Exemplary metabolic modifications are disclosed herein.

As used herein, the term “isolated” when used in reference to a microbial organism is intended to mean an organism that is substantially free of at least one component as the referenced microbial organism is found in nature. The term includes a microbial organism that is removed from some or all components as it is found in its natural environment. The term also includes a microbial organism that is removed from some or all components as the microbial organism is found in non-naturally occurring environments. Therefore, an isolated microbial organism is partly or completely separated from other substances as it is found in nature or as it is grown, stored or subsisted in non-naturally occurring environments. Specific examples of isolated microbial organisms include partially pure microbes, substantially pure microbes and microbes cultured in a medium that is non-naturally occurring.

As used herein, the terms “microbial,” “microbial organism” or “microorganism” are intended to mean any organism that exists as a microscopic cell that is included within the domains of archaea, bacteria or eukarya. Therefore, the term is intended to encompass prokaryotic or eukaryotic cells or organisms having a microscopic size and includes bacteria, archaea and eubacteria of all species as well as eukaryotic microorganisms such as yeast and fungi. The term also includes cell cultures of any species that can be cultured for the production of a biochemical.

As used herein, the term “CoA” or “coenzyme A” is intended to mean an organic cofactor or prosthetic group (nonprotein portion of an enzyme) whose presence is required for the activity of many enzymes (the apoenzyme) to form an active enzyme system. Coenzyme A functions in certain condensing enzymes, acts in acetyl or other acyl group transfer and in fatty acid synthesis and oxidation, pyruvate oxidation and in other acetylation.

As used herein, the term “ACP” or “acyl carrier protein” refers to any of the relatively small acidic proteins that are associated with the fatty acid synthase system of many organisms, from bacteria to plants. ACPs can contain one 4′-phosphopantetheine prosthetic group bound covalently by a phosphate ester bond to the hydroxyl group of a serine residue. The sulfhythylgroup of the 4′-phosphopantetheine moiety serves as an anchor to which acyl intermediates are (thio)esterified during fatty-acid synthesis. An example of an ACP is Escherichia coli ACP, a separate single protein, containing 77 amino-acid residues (8.85 kDa), wherein the phosphopantetheine group is linked to serine 36.

As used herein, the term “substantially anaerobic” when used in reference to a culture or growth condition is intended to mean that the amount of oxygen is less than about 10% of saturation for dissolved oxygen in liquid media. The term also is intended to include sealed chambers of liquid or solid medium maintained with an atmosphere of less than about 1% oxygen.

“Exogenous” as it is used herein is intended to mean that the referenced molecule or the referenced activity is introduced into the host microbial organism. The molecule can be introduced, for example, by introduction of an encoding nucleic acid into the host genetic material such as by integration into a host chromosome or as non-chromosomal genetic material such as a plasmid. Therefore, the term as it is used in reference to expression of an encoding nucleic acid refers to introduction of the encoding nucleic acid in an expressible form into the microbial organism. When used in reference to a biosynthetic activity, the term refers to an activity that is introduced into the host reference organism. The source can be, for example, a homologous or heterologous encoding nucleic acid that expresses the referenced activity following introduction into the host microbial organism. Therefore, the term “endogenous” refers to a referenced molecule or activity that is present in the host. Similarly, the term when used in reference to expression of an encoding nucleic acid refers to expression of an encoding nucleic acid contained within the microbial organism. The term “heterologous” refers to a molecule or activity derived from a source other than the referenced species whereas “homologous” refers to a molecule or activity derived from the host microbial organism. Accordingly, exogenous expression of an encoding nucleic acid of the invention can utilize either or both a heterologous or homologous encoding nucleic acid.

It is understood that when more than one exogenous nucleic acid is included in a microbial organism that the more than one exogenous nucleic acids refers to the referenced encoding nucleic acid or biosynthetic activity, as discussed above. It is further understood, as disclosed herein, that such more than one exogenous nucleic acids can be introduced into the host microbial organism on separate nucleic acid molecules, on polycistronic nucleic acid molecules, or a combination thereof, and still be considered as more than one exogenous nucleic acid. For example, as disclosed herein a microbial organism can be engineered to express two or more exogenous nucleic acids encoding a desired pathway enzyme or protein. In the case where two exogenous nucleic acids encoding a desired activity are introduced into a host microbial organism, it is understood that the two exogenous nucleic acids can be introduced as a single nucleic acid, for example, on a single plasmid, on separate plasmids, can be integrated into the host chromosome at a single site or multiple sites, and still be considered as two exogenous nucleic acids. Similarly, it is understood that more than two exogenous nucleic acids can be introduced into a host organism in any desired combination, for example, on a single plasmid, on separate plasmids, can be integrated into the host chromosome at a single site or multiple sites, and still be considered as two or more exogenous nucleic acids, for example three exogenous nucleic acids. Thus, the number of referenced exogenous nucleic acids or biosynthetic activities refers to the number of encoding nucleic acids or the number of biosynthetic activities, not the number of separate nucleic acids introduced into the host organism.

As used herein, the term “gene disruption,” or grammatical equivalents thereof, is intended to mean a genetic alteration that renders the encoded gene product inactive or attenuated. The genetic alteration can be, for example, deletion of the entire gene, deletion of a regulatory sequence required for transcription or translation, deletion of a portion of the gene which results in a truncated gene product, or by any of various mutation strategies that inactivate or attenuate the encoded gene product, for example, replacement of a gene's promoter with a weaker promoter, replacement or insertion of one or more amino acid of the encoded protein to reduce its activity, stability or concentration, or inactivation of a gene's transactivating factor such as a regulatory protein. One particularly useful method of gene disruption is complete gene deletion because it reduces or eliminates the occurrence of genetic reversions in the non-naturally occurring microorganisms of the invention. A gene disruption also includes a null mutation, which refers to a mutation within a gene or a region containing a gene that results in the gene not being transcribed into RNA and/or translated into a functional gene product. Such a null mutation can arise from many types of mutations including, for example, inactivating point mutations, deletion of a portion of a gene, entire gene deletions, or deletion of chromosomal segments.

As used herein, the term “growth-coupled” when used in reference to the production of a biochemical product is intended to mean that the biosynthesis of the referenced biochemical product is produced during the growth phase of a microorganism. In a particular embodiment, the growth-coupled production can be obligatory, meaning that the biosynthesis of the referenced biochemical is an obligatory product produced during the growth phase of a microorganism.

As used herein, the term “attenuate,” or grammatical equivalents thereof, is intended to mean to weaken, reduce or diminish the activity or amount of an enzyme or protein. Attenuation of the activity or amount of an enzyme or protein can mimic complete disruption if the attenuation causes the activity or amount to fall below a critical level required for a given pathway to function. However, the attenuation of the activity or amount of an enzyme or protein that mimics complete disruption for one pathway, can still be sufficient for a separate pathway to continue to function. For example, attenuation of an endogenous enzyme or protein can be sufficient to mimic the complete disruption of the same enzyme or protein for production of a fatty alcohol, fatty aldehyde or fatty acid product of the invention, but the remaining activity or amount of enzyme or protein can still be sufficient to maintain other pathways, such as a pathway that is critical for the host microbial organism to survive, reproduce or grow. Attenuation of an enzyme or protein can also be weakening, reducing or diminishing the activity or amount of the enzyme or protein in an amount that is sufficient to increase yield of a fatty alcohol, fatty aldehyde or fatty acid product of the invention, but does not necessarily mimic complete disruption of the enzyme or protein.

The term “fatty alcohol,” as used herein, is intended to mean an aliphatic compound that contains one or more hydroxyl groups and contains a chain of 4 or more carbon atoms. The fatty alcohol possesses the group —CH₂OH that can be oxidized so as to form a corresponding aldehyde or acid having the same number of carbon atoms. A fatty alcohol can also be a saturated fatty alcohol, an unsaturated fatty alcohol, a 1,3-diol, or a 3-oxo-alkan-1-ol. Exemplary fatty alcohols include a compound of Formula (BI)-(VI):

wherein R₁ is a C₁₋₂₄ linear alkyl.

The term “fatty aldehyde,” as used herein, is intended to mean an aliphatic compound that contains an aldehyde (CHO) group and contains a chain of 4 or more carbon atoms. The fatty aldehyde can be reduced to form the corresponding alcohol or oxidized to form the carboxylic acid having the same number of carbon atoms. A fatty aldehyde can also be a saturated fatty aldehyde, an unsaturated fatty aldehyde, a 3-hydroxyaldehyde or 3-oxoaldehyde. Exemplary fatty aldehydes include a compound of Formula (VII)-(X):

wherein R₁ is a C₁₋₂₄ linear alkyl.

The term “fatty acid,” as used herein, is intended to mean an aliphatic compound that contains a carboxylic acid group and contains a chain of 4 or more carbon atoms. The fatty acid can be reduced to form the corresponding alcohol or aldehyde having the same number of carbon atoms. A fatty acid can also be a saturated fatty acid, an unsaturated fatty acid, a 3-hydroxyacid or a 3-oxoacids. Exemplary fatty acids include a compound of Formula (XI)-(XIV):

wherein R₁ is a C₁₋₂₄ linear alkyl.

The term “alkyl” refers to a linear saturated monovalent hydrocarbon. The alkyl can be a linear saturated monovalent hydrocarbon that has 1 to 24 (C₁₋₂₄), 1 to 17 (C₁₋₁₇), or 9 to 13 (C₉₋₁₃) carbon atoms. Examples of alkyl groups include, but are not limited to, methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, octyl, nonyl, decyl, undecyl and dodecyl. For example, C₉₋₁₃ alkyl refers to a linear saturated monovalent hydrocarbon of 9 to 13 carbon atoms.

As used herein, “isopropanol” is intended to mean a secondary alcohol, with the molecular formula of C₃H₈O and a molecular mass of 60.1 g/mol, wherein the alcohol carbon is attached to two other carbons. This attachment is sometimes shown as (CH₃)₂CHOH. Isopropanol is also known in the art as propan-2-ol, 2-propanol or the abbreviation IPA. Isopropanol is an isomer of n-propanol.

As used herein, the phrase “enhance carbon flux” is intended to mean to intensify, increase, or further improve the extent or flow of metabolic carbon through or to a desired pathway, pathway product, intermediate, or compound. The intensity, increase or improvement can be relative to a predetermined baseline of a pathway product, intermediate or compound. For example, an increased yield of acetyl-CoA can be achieved per mole of methanol with a phosphoketolase enzyme described herein (see, e.g., FIG. 1) than in the absence of a phosphoketolase enzyme. Similarly, an increased yield of acetyl-CoA can be achieved per mole of methanol with the formale assimilation enzymes (see, e.g., FIG. 1) than in the absence of the enzymes. Since an increased yield of acetyl-CoA can be achieved, a higher yield of acetyl-CoA derived products, such as fatty alcohols, fatty acids, fatty aldehydes or isopropanol of the invention, can also be achieved.

Provided herein are methanol metabolic pathways and a methanol oxidation pathway to improve that availability of reducing equivaments and/or substrants for production of a compound of the invention. Because methanol is a relatively inexpensive organic feedstock that can be used as a redox, energy, and carbon source for the production of chemicals such as fatty alcohols, fatty acids, fatty aldehydes or isopropanol, and their intermediates, it is a desireable substrate for the non-naturally occurring microbial organisms of the invention. Employing one or more methanol metabolic enzymes as described herein, for example as shown in FIGS. 1 and 10, methanol can enter central metabolism in most production hosts by employing methanol dehydrogenase (FIG. 1, step A) along with a pathway for formaldehyde assimilation. One exemplary formaldehyde assimilation pathway that can utilize formaldehyde produced from the oxidation of methanol is shown in FIG. 1, which involves condensation of formaldehyde and D-ribulose-5-phosphate to form hexulose-6-phosphate (H6P) by hexulose-6-phosphate synthase (FIG. 1, step B). The enzyme can use Mg²⁺ or Mn²⁺ for maximal activity, although other metal ions are useful, and even non-metal-ion-dependent mechanisms are contemplated. H6P is converted into fructose-6-phosphate by 6-phospho-3-hexuloisomerase (FIG. 1, step C). Another exemplary pathway that involves the detoxification and assimilation of formaldehyde produced from the oxidation of methanol proceeds through dihydroxyacetone. Dihydroxyacetone synthase (FIG. 1, step D) is a transketolase that first transfers a glycoaldehyde group from xylulose-5-phosphate to formaldehyde, resulting in the formation of dihydroxyacetone (DHA) and glyceraldehyde-3-phosphate (G3P), which is an intermediate in glycolysis. The DHA obtained from DHA synthase can be then further phosphorylated to form DHA phosphate by a DHA kinase. DHAP can be assimilated into glycolysis, e.g. via isomerization to G3P, and several other pathways. Alternatively, DHA and G3P can be converted by fructose-6-phosphate aldolase to form fructose-6-phosphate (F6P).

By combining the pathways for methanol oxidation (FIG. 1, step A) and formaldehyde fixation (FIG. 1, Steps B and C or Step D), molar yields of 0.333 mol acetyl-CoA/mol methanol can be achieved for production of a fatty alcohol, a fatty acid, a fatty aldehyde, isopropanol, and their intermediates. The following maximum theoretical yield stoichiometries for a fatty alcohol (e.g., a C12), a fatty acid (e.g., a C12), a fatty aldehyde (e.g., a C12), isopropanol are thus made possible by combining the steps for methanol oxidation, formaldehyde fixation, and product synthesis.

18CH₄O+9O₂→C₁₂H₂₆O+6CO₂+23H₂O (Fatty Alcohol on MeOH)

18CH₄O+10O₂→C₁₂H₂₄O₂+6CO₂+24H₂O (Fatty Acid on MeOH)

18CH₄O+9.5O₂→C₁₂H₂₄O+6CO₂+24H₂O (Fatty Aldehyde on MeOH)

6CH₄O+4.5O₂→C₃H₈O+3CO₂+8H₂O (Isopropanol on MeOH)

The yield on several substrates, including methanol, can be further increased by capturing some of the carbon lost from the conversion of pathway intermediates, e.g. pyruvate to acetyl-CoA, using one of the formate reutilization pathways shown in FIG. 1. For example, the CO₂ generated by conversion of pyruvate to acetyl-CoA (FIG. 1, step R) can be converted to formate via formate dehydrogenase (FIG. 1, step S). Alternatively, pyruvate formate lyase, which forms formate directly instead of CO₂, can be used to convert pyruvate to acetyl-CoA (FIG. 1, step Q). Formate can be converted to formaldehyde by using: 1) formate reductase (FIG. 1, step E), 2) a formyl-CoA synthetase, transferase, or ligase along with formyl-CoA reductase (FIG. 1, steps F-G), or 3) formyltetrahydrofolate synthetase, methenyltetrahydrofolate cyclohydrolase, methylenetetrahydrofolate dehydrogenase, and formaldehyde-forming enzyme (FIG. 1, steps H-I-J-K). Conversion of methylene-THF to formaldehyde alternatively will occur spontaneously. Alternatively, formate can be reutilized by converting it to pyruvate or acetyl-CoA using FIG. 1, steps H-I-J-L-M-N or FIG. 1, steps H-I-J-O-P, respectively. Formate reutilization is also useful when formate is an external carbon source. For example, formate can be obtained from organocatalytic, electrochemical, or photoelectrochemical conversion of CO2 to formate. An alternative source of methanol for use in the present methods is organocatalytic, electrochemical, or photoelectrochemical conversion of CO2 to methanol,

By combining the pathways for methanol oxidation (FIG. 1, step A), formaldehyde fixation (FIG. 1, Steps B and C or Step D), and formate reutilization, molar yields as high as 0.500 mol acetyl-CoA/mol methanol can be achieved for production of a fatty alcohol, a fatty acid, a fatty aldehyde, isopropanol, and their intermediates. Thus, for example, the following maximum theoretical yield stoichiometries for a fatty alcohol (e.g., a C12), a fatty acid (e.g., a C12), a fatty aldehyde (e.g., a C12), and isopropanol are thus made possible by combining the steps for methanol oxidation, formaldehyde fixation, formate reutilization, and product synthesis.

12CH₄O→C₁₂H₂₆O+11H₂O (Fatty Alcohol on MeOH)

12CH₄O+O₂→C₁₂H₂₄O₂+12H₂O (Fatty Acid on MeOH)

12CH₄O+0.5O₂→C₁₂H₂₄O+12H₂O (Fatty Aldehyde on MeOH)

4CH₄O+1.5O₂→C₃H₈O+4H₂O+CO₂ (Isopropanol on MeOH)

By combining pathways for formaldehyde fixation and formate reutilization, yield increases on additional substrates are also available including but not limited to glucose, glycerol, sucrose, fructose, xylose, arabinose and galactose. For example, the following maximum theoretical yield stoichiometries for a fatty alcohol (e.g., a C12), a fatty acid (e.g., a C12), a fatty aldehyde (e.g., a C12), and isopropanol on glucose are made possible by combining the steps for formaldehyde fixation, formate reutilization, and product synthesis.

3C₆H₁₂O₆→C₁₂H₂₆O+5H₂O+6CO₂ (Fatty Alcohol on glucose)

3C₆H₁₂O₆→1.0588C₁₂H₂₄O₂+5.2941H₂O+5.2941CO₂ (Fatty Acid on glucose)

3C₆H₁₂O₆→1.0286C₁₂H₂₄O+5.6571H₂O+5.6571CO₂ (Fatty Aldehyde on glucose)

C₆H₁₂O₆→1.3333C₃H₈O+0.6667H₂O+2CO₂ (Isopropanol on glucose)

Similarly, the maximum theoretical yield of a fatty alcohol, a fatty acid, a fatty aldehyde, or isopropanol from glycerol can be increased by enabling fixation of formaldehyde from generation and utilization of formate. The following maximum theoretical yield stoichiometries for a fatty alcohol (e.g., a C12), a fatty acid (e.g., a C12), a fatty aldehyde (e.g., a C12), and isopropanol on glycerol are thus made possible by combining the steps for formaldehyde fixation, formate reutilization, and product synthesis.

6C₃H₈O₃→1.1667C₁₂H₂₆O+8.8333H₂O+4CO₂ (Fatty Alcohol on glycerol)

6C₃H₈O₃→1.2353C₁₂H₂₄O₂+9.1765H₂O+3.1765CO₂ (Fatty Acid on glycerol)

6C₃H₈O₃→1.2000C₁₂H₂₄O+9.6000H₂O+3.6000CO₂ (Fatty Aldehyde on glycerol)

C₃H₈O₃→0.7778C₃H₈O+0.8889H₂O+0.6667CO₂ (Isopropanol on glycerol)

In numerous engineered pathways, product yields based on carbohydrate feedstock are hampered by insufficient reducing equivalents or by loss of reducing equivalents to byproducts. Methanol is a relatively inexpensive organic feedstock that can be used to generate reducing equivalents by employing one or more methanol metabolic enzymes as shown in FIG. 10. Reducing equivalents can also be extracted from hydrogen and carbon monoxide by employing hydrogenase and carbon monoxide dehydrogenase enzymes, respectively, as shown in FIG. 10. The reducing equivalents are then passed to acceptors such as oxidized ferredoxins, oxidized quinones, oxidized cytochromes, NAD(P)+, water, or hydrogen peroxide to form reduced ferredoxin, reduced quinones, reduced cytochromes, NAD(P)H, H₂, or water, respectively. Reduced ferredoxin, reduced quinones and NAD(P)H are particularly useful as they can serve as redox carriers for various Wood-Ljungdahl pathway, reductive TCA cycle, or product pathway enzymes.

The reducing equivalents produced by the metabolism of methanol, hydrogen, and carbon monoxide can be used to power several fatty alcohol, fatty acid, fatty aldehyde, and isopropanol production pathways. For example, the maximum theoretical yield of a fatty alcohol, a fatty acid, a fatty aldehyde, or isopropanol from glucose and glycerol can be increased by enabling fixation of formaldehyde, formate reutilization, and extraction of reducing equivalents from an external source such as hydrogen. In fact, by combining pathways for formaldehyde fixation, formate reutilization, reducing equivalent extraction, and product synthesis, the following maximum theoretical yield stoichiometries for fatty alcohol, a fatty acid, a fatty aldehyde, and isopropanol on glucose and glycerol are made possible.

2C₆H₁₂O₆+12H₂→C₁₂H₂₆O+11H₂O (Fatty Alcohol on glucose+external redox)

2C₆H₁₂O₆+10H₂→C₁₂H₂₄O₂+10H₂O (Fatty Acid on glucose+external redox)

2C₆H₁₂O₆+11H₂→C₁₂H₂₄O+11H₂O (Fatty Aldehyde on glucose+external redox)

C₆H₁₂O₆+6H₂→2C₃H₈O+4H₂O (Isopropanol on glucose+external redox)

4C₃H₈O₃+8H₂→C₁₂H₂₆O+11H₂O (Fatty Alcohol on glycerol+external redox)

4C₃H₈O₃+6H₂→C₁₂H₂₄O₂+10H₂O (Fatty Acid on glycerol+external redox)

4C₃H₈O₃+7H₂→C₁₂H₂₄O+11H₂O (Fatty Aldehyde on glycerol+external redox)

C₃H₈O₃+2H₂→C₃H₈O+2H₂O (Isopropanol on glycerol+external redox)

In most instances, achieving such maximum yield stoichiometries may require some oxidation of reducing equivalents (e.g., H₂+½ O₂→H₂O, CO+½ O₂→CO₂, CH₄O+1.5 O₂→CO₂+2 H₂O, C₆H₁₂O₆+6 O₂→6 CO₂+6 H₂O) to provide sufficient energy for the substrate to product pathways to operate. Nevertheless, if sufficient reducing equivalents are available, enabling pathways for fixation of formaldehyde, formate reutilization, extraction of reducing equivalents, and product synthesis can even lead to production of a fatty alcohol, a fatty acid, a fatty aldehyde, isopropanol, and their intermediates, directly from CO₂.

Pathways identified herein, and particularly pathways exemplified in specific combinations presented herein, are superior over other pathways based in part on the applicant's ranking of pathways based on attributes including maximum theoretical compound yield, maximal carbon flux, maximal production of reducing equivalents, minimal production of CO2, pathway length, number of non-native steps, thermodynamic feasibility, number of enzymes active on pathway substrates or structurally similar substrates, and having steps with currently characterized enzymes, and furthermore, the latter pathways are even more favored by having in addition at least the fewest number of non-native steps required, the most enzymes known active on pathway substrates or structurally similar substrates, and the fewest total number of steps from central metabolism.

In some embodiments, the microorganisms of the invention can utilize a heterologous MI-FAE cycle and/or a MD-FAE cycle coupled with an acyl-CoA termination pathway to form fatty alcohols, fatty aldehydes, or fatty acids. The MI-FAE cycle can include a thiolase, a 3-oxoacyl-CoA reductase, a 3-hydroxyacyl-CoA dehydratase and an enoyl-CoA reductase. The MD-FAE cycle can include an elongase, a 3-oxoacyl-CoA reductase, a 3-hydroxyacyl-CoA dehydratase and an enoyl-CoA reductase. Each passage through the MI-FAE cycle and/or the MD-FAE cycle results in the formation of an acyl-CoA elongated by a single two carbon unit compared to the acyl-CoA substrate entering the elongation cycle. Products can be even or odd chain length, depending on the initial substrate entering the acyl-CoA elongation pathway, i.e. two acety-CoA substrates, malonyl-CoA or one acetyl-CoA substrate combined with a propionyl-CoA substrate. Elongation of the two acetyl-CoA substrates or malonyl-CoA produces an even chain length product, whereas elongation with the propionyl-CoA substrate produces an odd chain length product. A termination pathway catalyzes the conversion of a MI-FAE intermediate and/or a MD-FAE intermediate, such as the acyl-CoA, to its corresponding fatty alcohol, fatty aldehyde, or fatty acid product MI-FAE cycle, MD-FAE cycle and termination pathway enzymes can be expressed in one or more compartments of the microorganism. For example, in one embodiment, all MI-FAE cycle and termination pathway enzymes are expressed in the cytosol. In another embodiment, all MD-FAE cycle and termination pathway enzymes are expressed in the cytosol. Additionally, the microorganisms of the invention can be engineered to optionally secret the desired product into the culture media or fermentation broth for further manipulation or isolation.

In some embodiments, the microorganisms of the invention can utilize a heterologous FAACPE cycle coupled with an acyl-ACP termination pathway to form fatty alcohols, fatty aldehydes, or fatty acids. The FAACPE cycle can include a β-ketoacyl-ACP synthase, a β-ketoacyl-ACP reductase, a β-hydroxyacyl-ACP reductase, and a enoyl ACP-reductase. Each passage through the FAACPE cycle results in the formation of an acyl-ACP elongated by a single two carbon unit compared to the acyl-ACP substrate entering the elongation cycle. Products can be even or odd chain length, depending on the initial substrate entering the FAACPE pathway, i.e. acetoacetyl-ACP or 3-oxovaleryl-ACP. Elongation of the acetoacetyl-ACP substrates produces an even chain length product, whereas elongation with the 3-oxovaleryl-ACP substrate produces an odd chain length product. A termination pathway catalyzes the conversion of a FAACPE intermediate, such as the acyl-ACP, to its corresponding fatty alcohol, fatty aldehyde, or fatty acid product. FAACPE cycle and termination pathway enzymes can be expressed in one or more compartments of the microorganism. For example, in one embodiment, all FAACPE cycle and termination pathway enzymes are expressed in the cytosol. Additionally, the microorganisms of the invention can be engineered to optionally secret the desired product into the culture media or fermentation broth for further manipulation or isolation.

Products of the invention include fatty alcohols, fatty aldehydes, or fatty acids derived from intermediates of the MI-FAE cycle, MD-FAE cycle, and/or FAACPE cycle. For example, alcohol products can include saturated fatty alcohols, unsaturated fatty alcohols, 1,3-diols, and 3-oxo-alkan-1-ols. Aldehyde products can include saturated fatty aldehydes, unsaturated fatty aldehydes, 3-hydroxyaldehydes and 3-oxoaldehydes. Acid products can include saturated fatty acids, unsaturated fatty acids, 3-hydroxyacids and 3-oxoacids. These products can further be converted to derivatives such as fatty esters, either by chemical or enzymatic means. Methods for converting fatty alcohols to esters are well known in the art. Another product of the invention is isopropanol.

The invention also encompasses fatty alcohol, fatty aldehyde, and fatty acid chain-length control strategies in conjunction with host strain engineering strategies, such that the non-naturally occurring microorganism of the invention efficiently directs carbon and reducing equivalents toward fermentation products of a specific chain length.

Recombinant microorganisms of the invention can produce commercial quantities of a fatty alcohol, fatty aldehyde, or fatty acid ranging in chain length from four carbon atoms (C₄) to twenty-four carbon atoms (C₂₄) or more carbon atoms. The microorganism of the invention can produce a desired product that is at least 50%, 60%, 70%, 75%, 85%, 90%, 95% or more selective for a particular chain length. The carbon chain-length of the product can be controlled by one or more enzymes of the MI-FAE cycle (steps A/B/C/D of FIG. 7) and/or one or more enzymes of the MD-FAE cycle (steps E/B/C/D of FIG. 7) in combination with one or more termination pathway enzymes (steps E-N of FIG. 8). Chain length can be capped during the elongation cycle by one or more MI-FAE cycle enzymes (thiolase, 3-oxoacyl-CoA reductase, 3-hydroxyacyl-CoA dehydratase and/or enoyl-CoA reductase) exhibiting selectivity for MI-FAE cycle substrates having a number of carbon atoms that are no greater than the desired product size. Alternatively, or in addition, chain length can be capped during the elongation cycle by one or more MD-FAE cycle enzymes (elongase, 3-oxoacyl-CoA reductase, 3-hydroxyacyl-CoA dehydratase and/or enoyl-CoA reductase). Chain length can be further constrained by one or more enzymes catalyzing the conversion of the MI-FAE cycle intermediate to the fatty alcohol, fatty aldehyde or fatty acid product such that the one or more termination enzymes only reacts with substrates having a number of carbon atoms that are no less than the desired fatty alcohol, fatty aldehyde or fatty acid product.

The termination pathway enzymes catalyzing conversion of a MI-FAE-CoA intermediate or MD-FAE-CoA intermediate to a fatty alcohol can include enzyme combinations of a fatty acyl-CoA reductase (alcohol or aldehyde forming), a fatty aldehyde reductase, an acyl-ACP reductase, an acyl-CoA:ACP acyltransferase, a thioesterase, an acyl-CoA hydrolase and/or a carboxylic acid reductase (see, e.g., pathways G; E/F; K/J/F; H/N/F; or K/L/N/F of FIG. 8). Termination pathway enzymes for converting a MI-FAE-CoA intermediate or MD-FAE-CoA intermediate to a fatty acid can include enzyme combinations of a thioesterase, a CoA hydrolase, an acyl-CoA:ACP acyltransferase, an aldehyde dehydrogenase and/or an acyl-ACP reductase (see, e.g., pathways H; K/L; EN; K/J/N of FIG. 8). For production of a fatty aldehyde, the termination pathway enzymes can include enzyme combinations of a fatty acyl-CoA reductase (aldehyde forming), an acyl-ACP reductase, an acyl-CoA:ACP acyltransferase, a thioesterase, an acyl-CoA hydrolase and/or a carboxylic acid reductase (see, e.g., pathways E; K/J; H/N; or K/L/N of FIG. 8).

The carbon chain-length of the product can also be controlled by one or more enzymes of the FAACPE cycle (steps H/E/F/G of FIG. 12) in combination with one or more termination pathway enzymes (steps I-O of FIG. 12). Chain length can be capped during the elongation cycle by one or more FAACPE cycle enzymes (β-ketoacyl-ACP synthase, β-ketoacyl-ACP reductase, β-hydroxyacyl-ACP reductase, and/or enoyl ACP-reductase) exhibiting selectivity for FAACPE cycle substrates having a number of carbon atoms that are no greater than the desired product size. Chain length can be further constrained by one or more enzymes catalyzing the conversion of the FAACPE cycle intermediate to the fatty alcohol, fatty aldehyde or fatty acid product such that the one or more termination enzymes only reacts with substrates having a number of carbon atoms that are no less than the desired fatty alcohol, fatty aldehyde or fatty acid product.

The termination pathway enzymes catalyzing conversion of a FAACPE cycle intermediate to a fatty alcohol can include enzyme combinations of a thioesterase, a fatty acyl-ACP reductase, an acyl-CoA synthase, an acyl-CoA reductase, a fatty aldehyde reductase, a fatty alcohol forming acyl-CoA reductase (FAR), and/or a carboxylic acid reductase (CAR) (see, e.g., pathways J/M; I/K/L/M; I/O/M; and I/K/N of FIG. 12). Termination pathway enzyme for converting a FAACPE intermediate to a fatty acid can include a thioesterase (see, e.g., pathways I of FIG. 12). For production of a fatty aldehyde, the termination pathway enzymes can include combinations of a thioesterase, a fatty acyl-ACP reductase, an acyl-CoA synthase, an acyl-CoA reductase, a fatty aldehyde reductase, and/or a carboxylic acid reductase (CAR), (see, e.g., pathways J; I/K/L; and I/O of FIG. 12).

The non-naturally occurring microbial organisms of the invention can also efficiently direct cellular resources, including carbon, energy and reducing equivalents, to the production of fatty alcohols, fatty aldehydes and fatty acids, thereby resulting in improved yield, productivity and/or titer relative to a naturally occurring organism. In one embodiment, the microorganism is modified to increase cytosolic acetyl-CoA levels. In another embodiment, the microorganism is modified to efficiently direct cytosolic acyl-CoA into fatty alcohols, fatty aldehydes or fatty acids rather than other byproducts or cellular processes Enzymes or pathways that lead to the formation of byproducts can be attenuated or deleted. Exemplary byproducts include, but are not limited to, ethanol, glycerol, lactate, acetate, esters and carbon dioxide. Additional byproducts can include fatty-acyl-CoA derivatives such as alcohols, alkenes, alkanes, esters, acids and aldehydes. Accordingly, a byproduct can include any fermentation product diverting carbon and/or reducing equivalents from the product of interest.

In another embodiment, the availability of reducing equivalents or redox ratio is increased. In yet another embodiment, the cofactor requirements of the microorganism are balanced such that the same reduced cofactors generated during carbon assimilation and central metabolism are utilized by MI-FAE cycle, MD-FAE cycle and/or termination pathway enzymes. In yet another embodiment, the fatty alcohol, fatty aldehyde or fatty acid producing organism expresses a transporter which exports the fatty alcohol, fatty aldehyde or fatty acid from the cell.

Microbial organisms capable of fatty alcohol production are exemplified herein with reference to the Saccharomyces cerevisiae genetic background. However, with the complete genome sequence available now for thousands of species (with more than half of these available on public databases such as the NCBI), the identification of an alternate species homolog for one or more genes, including for example, orthologs, paralogs and nonorthologous gene displacements, and the interchange of genetic alterations between eukaryotic organisms is routine and well known in the art. Accordingly, the metabolic alterations enabling production of fatty alcohols described herein with reference to a particular organism such as Saccharomyces cerevisiae can be readily applied to other microorganisms. Given the teachings and guidance provided herein, those skilled in the art understand that a metabolic alteration exemplified in one organism can be applied equally to other organisms.

The methods of the invention are applicable to various prokaryotic and eukaryotic organisms such as bacteria, yeast and fungus. For example, the yeast can include Saccharomyces cerevisiae and Rhizopus arrhizus. Exemplary eukaryotic organisms can also include Crabtree positive and negative yeasts, and yeasts in the genera Saccharomyces, Kluyveromyces, Candida or Pichia. Further exemplary eukaryotic species include those selected from Schizosaccharomyces pombe, Kluyveromyces lactis, Kluyveromyces marxianus, Aspergillus terreus, Aspergillus niger, Rhizopus arrhizus, Rhizopus oryzae, Candida albicans, Candida boidinii, Candida sonorensis, Candida tropicalis, Yarrowia lipolytica and Pichia pastoris. Additionally, select cells from larger eukaryotic organisms are also applicable to methods of the present invention. Exemplary bacteria include species selected from Escherichia coli, Klebsiella oxytoca, Anaerobiospirillum succiniciproducens, Actinobacillus succinogenes, Mannheimia succiniciproducens, Rhizobium etli, Bacillus subtilis, Corynebacterium glutamicum, Gluconobacter oxydans, Zymomonas mobilis, Lactococcus lactis, Lactobacillus plantarum, Streptomyces coelicolor, Clostridium acetobutylicum, Pseudomonas fluorescens, and Pseudomonas putida.

In some aspects of the invention, production of fatty alcohols, fatty aldehydes and fatty acids through the MI-FAE cycle and termination pathways disclosed herein are particularly useful because the cycle and pathways result in higher product and ATP yields than through naturally occurring biosynthetic pathways such as the well-known malonyl-CoA dependent fatty acid synthesis pathway, or in some aspects the malonyl-ACP dependent fatty acid synthesis pathway. For example, using acetyl-CoA as a C₂ extension unit (e.g. step A, FIG. 2) instead of malonyl-acyl carrier protein (malonyl-ACP) saves one ATP molecule per unit flux of acetyl-CoA entering the MI-FAE cycle. The MI-FAE cycle results in acyl-CoA instead of acyl-ACP, and can preclude the need of the ATP-consuming acyl-CoA synthase reactions for the production of octanol and other fatty alcohols, fatty aldehydes or fatty acids if acetyl-CoA is used as the extender unit. The fatty alcohol, fatty aldehyde and fatty acid producing organisms of the invention can additionally allow the use of biosynthetic processes to convert low cost renewable feedstock for the manufacture of chemical products.

The eukaryotic organism of the invention can be further engineered to metabolize and/or co-utilize a variety of feedstocks including glucose, xylose, fructose, syngas, methanol, and the like.

Chain length control can be achieved using a combination of highly active enzymes with suitable substrate ranges appropriate for biosynthesis of the desired fatty alcohol, fatty aldehyde, or fatty acid. Chain length of the product can be controlled using one or more enzymes of MI-FAE cycle, MD-FAE cycle, FAACPE cycle or termination pathway. As described herein, chain length can be capped during the MI-FAE cycle by one or more MI-FAE cycle enzymes (thiolase, 3-oxoacyl-CoA reductase, 3-hydroxyacyl-CoA dehydratase and/or enoyl-CoA reductase), in the case of the MD-FAE cycle, one or more MD-FAE cycle enzymes (elongase, 3-oxoacyl-CoA reductase, 3-hydroxyacyl-CoA dehydratase and/or enoyl-CoA reductase), and in the case of the FAACPE cycle, one or more enzymes (β-ketoacyl-ACP synthase, β-ketoacylcl-ACP reductase, β-hydroxyacyl-ACP reductase and/or enoyl ACP-reductase), exhibiting selectivity for MI-FAE cycle, MD-FAE cycle and/or FAACPE cycle substrates having a number of carbon atoms that are no greater than the desired product size. Since enzymes are reversible, any of the elongation pathway enzymes can serve in this capacity. Selecting enzymes with broad substrate ranges but defined chain-length boundaries enables the use of a single enzyme to catalyze multiple cycles of elongation, while conferring product specificity. To further hone specificity and prevent the accumulation of shorter byproducts, selectivity is further constrained by product-forming termination enzymes, such that one or more enzymes are selective for acyl-CoA, acyl-ACP or other termination pathway substrates having a number of carbon atoms that are no less than the desired chain length. The deletion or attenuation of endogenous pathway enzymes that produce different chain length products can further hone product specificity.

Using the approaches outlined herein, one skilled in the art can select enzymes from the literature with characterized substrate ranges that selectively produce a fatty alcohol, fatty aldehyde or fatty acid product of a specific chain length. To selectively produce fatty alcohols, fatty aldehydes or fatty acids of a desired length, one can utilize combinations of known enzymes in the literature with different selectivity ranges as described above. For example, a non-naturally occurring microbial organism that produces C₁₆ fatty alcohol can express enzymes such as the Rattus norvegicus Acaala thiolase and the enoyl-CoA reducatse of Mycobacterium smegmatis, which only accept substrates up to length C₁₆. Coupling one or both chain elongation enzymes with a C₁₆-C₁₈ fatty acyl-CoA reductase (alcohol or aldehyde forming) such as FAR of Simmondsia chinensis further increases product specificity by reducing the synthesis of shorter alcohol products. As another example, a non-naturally occurring microbial organism of the invention can selectively produce alcohols of length C₁₄ by combining the 3-hydroxyacyl-CoA dehydratase of Arabidopsis thaliana with the acyl-CoA reductase Acrl of Acinetobacter sp. Strain M-1. To produce 3-oxoacids of length C₁₄, one can, for example, combine the rat thiolase with the 3-oxoacyl-CoA hydrolase of Solanum lycopersicum. As still a further example, to produce C₁₈ fatty acids, one can combine the Salmonella enterica fadE enoyl-CoA reductase with the tesB thioesterase of E. coli. In yet another example, selective production of C₆ alcohols are formed by combining the paaH1 thiolase from Ralstonia eutropha with the Leifsonia sp. S749 alcohol dehydrogenase lsadh.

Exemplary MI-FAE cycle, MD-FAE cycle and termination pathway enzymes are described in detail in Example IV. The biosynthetic enzymes described herein exhibit varying degrees of substrate specificity. Exemplary substrate ranges of enzymes characterized in the literature are shown in the table below and described in further detail in Example IV.

Pathway step Chain length Gene Organism 2A C4 AtoB Escherichia coli 2A C6 PhaD Pseudomonas putida 2A C6-C8 BktB Ralstonia eutropha 2A C10-C16 Acaa1a Rattus norvegicus 2B C4 Hbd Clostridium acetobutylicum 2B C4-C6 paaH1 Ralstonia eutropha 2B  C4-C10 HADH Sus scrofa 2B/C  C4-C18 FadB Escherichia coli 2B/C  C4-C18 Fox2 Candida tropicalis 2B/C  C4-C18 Fox2 Saccharomyces cerevisiae 2C C4-C6 crt Clostridium acetobutylicum 2C C4-C7 pimF Rhodopseudomonas palustris 2C  C4-C14 MFP2 Arabidopsis thaliana 2D C4-C6 ECR1 Euglena gracilis 2D C6-C8 ECR3 Euglena gracilis 2D C8-10  ECR2 Euglena gracilis 2D  C8-C16 ECR Rattus norvegicus 2D C10-C16 ECR Mycobacterium smegmatis 2D  C2-C18 fadE Salmonella enterica 2E C2-C4 bphG Pseudomonas sp 2E C4 Bld Clostridium saccharoperbutylacetonicum 2E C12-C20 ACR Acinetobacter calcoaceticus 2E C14-C18 Acr1 Acinetobacter sp. Strain M-1 2E C16-C18 Rv1543, Rv3391 Mycobacterium tuberculosis 2E  C18 FAR1, FAR2 Mus musculus 2E C12-C20 orf1594 Synechococcus elongatus PCC7942 2E  C6-C18 Maqu_2220 Marinobacter aquaeolei 2F C6-C7 lsadh Leifsonia sp. S749 2F C2-C8 yqhD Escherichia coli 2F  C3-C10 Adh Pseudomonas putida 2F  C2-C14 alrA Acinetobacter sp. strain M-1 2F  C2-C30 ADH1 Geobacillus thermodenitrificans 2F C3-C8 ADH6 Saccharomyces cerevisiae s288c 2G C2 adhE Escherichia coli 2G C2-C8 adhe2 Clostridium acetobutylicum 2G C14-C16 At3g11980 Arabidopsis thaliana 2G  C16 At3g44560 Arabidopsis thaliana 2G C16-C18 FAR Simmondsia chinensis 2H C4 Cat2 Clostridium kluyveri 2H C4-C6 Acot12 Rattus norvegicus 2H  C14 MKS2 Solanum lycopersicum 2L  C8-C10 fatB2 Cuphea hookeriana 2L  C12 fatB Umbellularia california 2L C14-C16 fatB3 Cuphea hookeriana 2L  C18 tesA Escherichia coli 2N C12-C18 Car Nocardia iowensis 2N C12-C16 Car Mycobacterium sp. (strain JLS) 2O C4-C8 ELO1 Trypanosoma brucei 2O C10-C12 ELO2 Trypanosoma brucei 2O C14-C16 ELO3 Trypanosoma brucei 2O C14-C16 ELO1 Saccharomyces cerevisiae 2O C18-C20 ELO2 Saccharomyces cerevisiae 2O C22-C24 ELO3 Saccharomyces cerevisiae

Taking into account the differences in chain-length specificities of each enzyme in the MI-FAE cycle, MD-FAE cycle or FAACPE cycle, one skilled in the art can select one or more enzymes for catalyzing each elongation cycle reaction step (e.g., steps A-D or steps E/B/C/D of FIG. 6, or H/E/F/G of FIG. 12). For example, for the thiolase step of the MI-FAE cycle, some thiolase enzymes such as bktB of Ralstonia eutropha catalyze the elongation of short- and medium-chain acyl-CoA intermediates (C₆-C₈), whereas others such as Acaa1a of R. norvegicus are active on longer-chain substrates (C₁₀-C₁₆). Thus, a microbial organism producing a fatty alcohol, fatty aldehyde or fatty acid can comprise one, two, three, four or more variants of a thiolase, elongase, 3-oxoacyl-CoA reductase, 3-hydroxyacyl-CoA dehydratase and/or enoyl-CoA reductase.

Chain length specificity of enzymes can be assayed by methods well known in the art (eg. Wrensford et al, Anal Biochem 192:49-54 (1991)). The substrate ranges of fatty alcohol, fatty aldehyde, or fatty acid producing enzymes can be further extended or narrowed by methods well known in the art. Variants of biologically-occurring enzymes can be generated, for example, by rational and directed evolution, mutagenesis and enzyme shuffling as described herein. As one example, a rational engineering approach for altering chain length specificity was taken by Denic and Weissman (Denic and Weissman, Cell 130:663-77 (2008)). Denic and Weissman mapped the region of the yeast elongase protein ELOp responsible for chain length, and introduced mutations to vary the length of fatty acid products. In this instance, the geometry of the hydrophobic substrate pocket set an upper boundary on chain length. A similar approach can be useful for altering the chain length specificities of enzymes of the MI-FAE cycle, MD-FAE cycle and/or termination pathways.

Enzyme mutagenesis, expression in a host, and screening for fatty alcohol production is another useful approach for generating enzyme variants with improved properties for the desired application. For example, US patent application 2012/0009640 lists hundreds of variants of Marinobacter algicola and Marinobacter aquaeolei FAR enzymes with improved activity over the wild type enzyme, and varying product profiles.

Enzyme mutagenesis (random or directed) in conjunction with a selection platform is another useful approach. For example, Machado and coworkers developed a selection platform aimed at increasing the activity of acyl-CoA elongation cycle enzymes on longer chain length substrates (Machado et al., Met Eng 14(5):504-511(2012)). Machado et al. identified the chain-length limiting step of their pathway (a 3-hydroxyacyl-CoA dehydrogenase) and evolved it for improved activity on C₆-C₈ substrates using an anaerobic growth rescue platform. Additional variants of enzymes useful for producing fatty alcohols are listed in the table below

Protein/ GenBankID/ Enzyme GI number Organism Variant(s) Reference 3-Ketoacyl-CoA Acaa2 Rattus H352A, H352E, Zeng et al., Prot. Expr. thiolase NP_569117.1 norvegicus H352K, H352Y Purif. 35: 320-326 GI:18426866 (2004) 3-Hydroxyacyl-CoA Hadh Rattus S137A, S137C, Liu et al., Prot. Expr. dehydrogenase NP_476534.1 norvegicus S137T Purif. 37: 344-351 GI:17105336 (2004). Enoyl-CoA Ech1 Rattus E144A, Kiema et al., hydratase NP_072116.1 norvegicus E144A/Q162L, Biochem. 38: 2991- GI:12018256 E164A, Q162A, 2999 (1999) Q162L, Q162M Enoyl-CoA InhA Mycobacterium K165A, K165Q, Poletto, S. et al., Prot. reductase AAY54545.1 tuberculosis Y158F Expr. Purif. 34: 118- GI:66737267 125 (2004). Acyl-CoA LuxC Photobacterium C171S, C279S, Lee, C. et al., Biochim. reductase AAT00788.1 phosphoreum C286S Biophys. Acta. 1338: GI:46561111 215-222 (1997). Alcohol YADH-1 Saccharomyces D223G, D49N, E68Q, Leskovac et al., FEMS dehydrogenase P00330.4 cerevisiae G204A, G224I, Yeast Res. 2(4): 481- GI:1168350 H47R, H51E, L203A 94 (2002). Fatty alcohol AdhE Escherichia coli A267T/E568K, Membrillo et al., forming acyl-CoA NP_415757.1 A267T J. Biol. Chem. 275(43): reductase (FAR) GI:16129202 333869-75 (2000).

Those skilled in the art will understand that the genetic alterations, including metabolic modifications exemplified herein, are described with reference to a suitable host organism such as E. coli or S. cerevisiae and their corresponding metabolic reactions or a suitable source organism for desired genetic material such as genes for a desired metabolic pathway. However, given the complete genome sequencing of a wide variety of organisms and the high level of skill in the area of genomics, those skilled in the art will readily be able to apply the teachings and guidance provided herein to essentially all other organisms. For example, the metabolic alterations exemplified herein can readily be applied to other species by incorporating the same or analogous encoding nucleic acid from species other than the referenced species. Such genetic alterations include, for example, genetic alterations of species homologs, in general, and in particular, orthologs, paralogs or nonorthologous gene displacements.

An ortholog is a gene or genes that are related by vertical descent and are responsible for substantially the same or identical functions in different organisms. For example, mouse epoxide hydrolase and human epoxide hydrolase can be considered orthologs for the biological function of hydrolysis of epoxides. Genes are related by vertical descent when, for example, they share sequence similarity of sufficient amount to indicate they are homologous, or related by evolution from a common ancestor. Genes can also be considered orthologs if they share three-dimensional structure but not necessarily sequence similarity, of a sufficient amount to indicate that they have evolved from a common ancestor to the extent that the primary sequence similarity is not identifiable. Genes that are orthologous can encode proteins with sequence similarity of about 25% to 100% amino acid sequence identity. Genes encoding proteins sharing an amino acid similarity less that 25% can also be considered to have arisen by vertical descent if their three-dimensional structure also shows similarities. Members of the serine protease family of enzymes, including tissue plasminogen activator and elastase, are considered to have arisen by vertical descent from a common ancestor.

Orthologs include genes or their encoded gene products that through, for example, evolution, have diverged in structure or overall activity. For example, where one species encodes a gene product exhibiting two functions and where such functions have been separated into distinct genes in a second species, the three genes and their corresponding products are considered to be orthologs. For the production of a biochemical product, those skilled in the art will understand that the orthologous gene harboring the metabolic activity to be introduced or disrupted is to be chosen for construction of the non-naturally occurring microorganism. An example of orthologs exhibiting separable activities is where distinct activities have been separated into distinct gene products between two or more species or within a single species. A specific example is the separation of elastase proteolysis and plasminogen proteolysis, two types of serine protease activity, into distinct molecules as plasminogen activator and elastase. A second example is the separation of mycoplasma 5′-3′ exonuclease and Drosophila DNA polymerase III activity. The DNA polymerase from the first species can be considered an ortholog to either or both of the exonuclease or the polymerase from the second species and vice versa.

In contrast, paralogs are homologs related by, for example, duplication followed by evolutionary divergence and have similar or common, but not identical functions. Paralogs can originate or derive from, for example, the same species or from a different species. For example, microsomal epoxide hydrolase (epoxide hydrolase I) and soluble epoxide hydrolase (epoxide hydrolase II) can be considered paralogs because they represent two distinct enzymes, co-evolved from a common ancestor, that catalyze distinct reactions and have distinct functions in the same species. Paralogs are proteins from the same species with significant sequence similarity to each other suggesting that they are homologous, or related through co-evolution from a common ancestor. Groups of paralogous protein families include HipA homologs, luciferase genes, peptidases, and others.

A nonorthologous gene displacement is a nonorthologous gene from one species that can substitute for a referenced gene function in a different species. Substitution includes, for example, being able to perform substantially the same or a similar function in the species of origin compared to the referenced function in the different species. Although generally, a nonorthologous gene displacement will be identifiable as structurally related to a known gene encoding the referenced function, less structurally related but functionally similar genes and their corresponding gene products nevertheless will still fall within the meaning of the term as it is used herein. Functional similarity requires, for example, at least some structural similarity in the active site or binding region of a nonorthologous gene product compared to a gene encoding the function sought to be substituted. Therefore, a nonorthologous gene includes, for example, a paralog or an unrelated gene.

Therefore, in identifying and constructing the non-naturally occurring microbial organisms of the invention having fatty alcohol, fatty aldehyde or fatty acid biosynthetic capability, those skilled in the art will understand with applying the teaching and guidance provided herein to a particular species that the identification of metabolic modifications can include identification and inclusion or inactivation of orthologs. To the extent that paralogs and/or nonorthologous gene displacements are present in the referenced microorganism that encode an enzyme catalyzing a similar or substantially similar metabolic reaction, those skilled in the art also can utilize these evolutionally related genes. Similarly for a gene disruption, evolutionally related genes can also be disrupted or deleted in a host microbial organism to reduce or eliminate functional redundancy of enzymatic activities targeted for disruption.

Orthologs, paralogs and nonorthologous gene displacements can be determined by methods well known to those skilled in the art. For example, inspection of nucleic acid or amino acid sequences for two polypeptides will reveal sequence identity and similarities between the compared sequences. Based on such similarities, one skilled in the art can determine if the similarity is sufficiently high to indicate the proteins are related through evolution from a common ancestor. Algorithms well known to those skilled in the art, such as Align, BLAST, Clustal W and others compare and determine a raw sequence similarity or identity, and also determine the presence or significance of gaps in the sequence which can be assigned a weight or score. Such algorithms also are known in the art and are similarly applicable for determining nucleotide sequence similarity or identity. Parameters for sufficient similarity to determine relatedness are computed based on well known methods for calculating statistical similarity, or the chance of finding a similar match in a random polypeptide, and the significance of the match determined. A computer comparison of two or more sequences can, if desired, also be optimized visually by those skilled in the art. Related gene products or proteins can be expected to have a high similarity, for example, 25% to 100% sequence identity. Proteins that are unrelated can have an identity which is essentially the same as would be expected to occur by chance, if a database of sufficient size is scanned (about 5%). Sequences between 5% and 24% may or may not represent sufficient homology to conclude that the compared sequences are related. Additional statistical analysis to determine the significance of such matches given the size of the data set can be carried out to determine the relevance of these sequences.

Exemplary parameters for determining relatedness of two or more sequences using the BLAST algorithm, for example, can be as set forth below. Briefly, amino acid sequence alignments can be performed using BLASTP version 2.0.8 (Jan. 5, 1999) and the following parameters: Matrix: 0 BLOSUM62; gap open: 11; gap extension: 1; x_dropoff: 50; expect: 10.0; wordsize: 3; filter: on. Nucleic acid sequence alignments can be performed using BLASTN version 2.0.6 (Sep. 16, 1998) and the following parameters: Match: 1; mismatch: −2; gap open: 5; gap extension: 2; x_dropoff: 50; expect: 10.0; wordsize: 11; filter: off. Those skilled in the art will know what modifications can be made to the above parameters to either increase or decrease the stringency of the comparison, for example, and determine the relatedness of two or more sequences.

In some embodiments, the invention provides a non-naturally occurring microbial organism having: (i) a formaldehyde fixation pathway; (ii) a formate assimilation pathway; and/or (iii) a methanol metabolic pathway as depicted in FIGS. 1 and 10, and a MI-FAE cycle or a MD-FAE cycle in combination with a termination pathway as depicted in FIGS. 2, 7 and 8, wherein said formaldehyde fixation pathway comprises. (1) 1B and 1C; (2) 1D; or (3) 1D and 1Z, wherein 1B is a 3-hexulose-6-phosphate synthase, wherein 1C is a 6-phospho-3-hexuloisomerase, wherein 1D is a dihydroxyacetone synthase, wherein 1Z is a fructose-6-phosphate aldolase, wherein said formate assimilation pathway comprises a pathway selected from: (4) 1E; (5) 1F, and 1G; (6) 1H, 1I, 1J, and 1K; (7) 1H, 1I, 1J, 1L, 1M, and 1N; (8) 1E, 1H, 1I, 1J, 1L, 1M, and 1N; (9) 1F, 1G, 1H, 1I, 1J, 1L, 1M, and 1N; (10) 1K, 1H, 1I, 1J, 1L, 1M, and 1N; and (11) 1H, 1I, 1J, 1O, and 1P, wherein 1E is a formate reductase, 1F is a formate ligase, a formate transferase, or a formate synthetase, wherein 1G is a formyl-CoA reductase, wherein 1H is a formyltetrahydrofolate synthetase, wherein 1I is a methenyltetrahydrofolate cyclohydrolase, wherein 1J is a methylenetetrahydrofolate dehydrogenase, wherein 1K is a formaldehyde-forming enzyme or spontaneous, wherein 1L is a glycine cleavage system, wherein 1M is a serine hydroxymethyltransferase, wherein 1N is a serine deaminase, wherein 1O is a methylenetetrahydrofolate reductase, wherein 1P is an acetyl-CoA synthase, wherein said methanol metabolic pathway comprises a pathway selected from: (12) 10J; (13) 10A, (14) 10A and 10B; (15) 10A, 10B and 10C; (16) 10J, 10K and 10C; (17) 10J, 10M, and 10N; (18) 10J and 10L; (19) 10J, 10L and 10G; (20) 10J, 10L, and 10I; (21) 10A, 10B, 10C, 10D, and 10E; (22) 10A, 10B, 10C, 10D, and 10F; (23) 10J, 10K, 10C, 10D, and 10E; (24) 10J, 10K, 10C, 10D, and 10F; (25) 10J, 10M, 10N, and 10O; (26) 10A, 10B, 10C, 10D, 10E, and 10G; (27) 10A, 10B, 10C, 10D, 10F, and 10G; (28) 10J, 10K, 10C, 10D, 10E, and 10G; (29) 10J, 10K, 10C, 10D, 10F, and 10G; (30) 10J, 10M, 10N, 10O, and 10G; (31) 10A, 10B, 10C, 10D, 10E, and 10I; (32) 10A, 10B, 10C, 10D, 10F, and 10I; (33) 10J, 10K, 10C, 10D, 10E, and 10I; (34) 10J, 10K, 10C, 10D, 10F, and 10I; and (35) 10J, 10M, 10N, 10O, and 10I, wherein 10A is a methanol methyltransferase, wherein 10B is a methylenetetrahydrofolate reductase, wherein 10C is a methylenetetrahydrofolate dehydrogenase, wherein 10D is a methenyltetrahydrofolate cyclohydrolase, wherein 10E is a formyltetrahydrofolate deformylase, wherein 10F is a formyltetrahydrofolate synthetase, wherein 10G is a formate hydrogen lyase, wherein 10I is a formate dehydrogenase, wherein 10J is a methanol dehydrogenase, wherein 10K is a formaldehyde activating enzyme or spontaneous, wherein 10L is a formaldehyde dehydrogenase, wherein 10M is a S-(hydroxymethyl)glutathione synthase or spontaneous, wherein 10N is a glutathione-dependent formaldehyde dehydrogenase, wherein 10O is a S-formylglutathione hydrolase, wherein the MI-FAE cycle includes one or more thiolase, one or more 3-oxoacyl-CoA reductase, one or more 3-hydroxyacyl-CoA dehydratase, and one or more enoyl-CoA reductase, wherein the MD-FAE cycle includes one or more elongase, one or more 3-oxoacyl-CoA reductase, one or more 3-hydroxyacyl-CoA dehydratase, and one or more enoyl-CoA reductase, wherein the termination pathway includes a pathway selected from: (36) 2H; (37) 2K and 2L; (38) 2E and 2N; (39) 2K, 2J, and 2N; (40) 2E; (41) 2K and 2J; (42) 2H and 2N; (43) 2K, 2L, and 2N; (44) 2E and 2F; (45) 2K, 2J, and 2F; (46) 2H, 2N, and 2F; (47) 2K, 2L, 2N, and 2F; (48) 2G; (49) 2P, wherein 2E is an acyl-CoA reductase (aldehyde forming), wherein 2F is an alcohol dehydrogenase, wherein 2G is an acyl-CoA reductase (alcohol forming), wherein 2H is an acyl-CoA hydrolase, acyl-CoA transferase or acyl-CoA synthase, wherein 2J is an acyl-ACP reductase, wherein 2K is an acyl-CoA:ACP acyltransferase, wherein 2L is a thioesterase, wherein 2N is an aldehyde dehydrogenase (acid forming) or a carboxylic acid reductase, wherein 2P is an acyl-ACP reductase (alcohol forming) wherein an enzyme of the formaldehyde fixation pathway, the formate assimilation pathway, the methanol metabolic pathway, the MI-FAE cycle, MD-FAE cycle or termination pathway is encoded by at least one exogenous nucleic acid and is expressed in a sufficient amount to produce a compound of Formula (I):

wherein R₁ is C₁₋₂₄ linear alkyl; R₂ is CH₂OH, CHO, or COOH; R₃ is H, OH, or oxo (═O); and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four, wherein the substrate of each of said enzymes of the MI-FAE cycle, the MD-FAE cycle and the termination pathway are independently selected from a compound of Formula (II), malonyl-CoA, propionyl-CoA or acetyl-CoA:

wherein R₁ is C₁₋₂₄ linear alkyl; R₃ is H, OH, or oxo (═O); R₄ is S-CoA, ACP, OH or H; and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four; wherein said one or more enzymes of the MI-FAE cycle are each selective for a compound of Formula (II) having a number of carbon atoms at R₁ that is no greater than the number of carbon atoms at R₁ of said compound of Formula (I), wherein said one or more enzymes of the MD-FAE cycle are each selective for a compound of Formula (II) having a number of carbon atoms at R₁ that is no greater than the number of carbon atoms at R₁ of said compound of Formula (I), and wherein said one or more enzymes of the termination pathway are each selective for a compound of Formula (II) having a number of carbon atoms at R₁ that is no less than the number of carbon atoms at R₁ of said compound of Formula (I).

In some embodiments, the invention provides a non-naturally occurring microbial organism having: (i) a formaldehyde fixation pathway; (ii) a formate assimilation pathway; and/or (iii) a methanol metabolic pathway as depicted in FIGS. 1 and 10, and a FAACPE cycle in combination with a termination pathway as depicted in FIG. 12, wherein said formaldehyde fixation pathway comprises. (1) 1B and 1C; (2) 1D; (3) 1D and 1Z, wherein 1B is a 3-hexulose-6-phosphate synthase, wherein 1C is a 6-phospho-3-hexuloisomerase, wherein 1D is a dihydroxyacetone synthase, wherein 1Z is a fructose-6-phosphate aldolase, wherein said formate assimilation pathway comprises a pathway selected from: (4) 1E; (5) 1F, and 1G; (6) 1H, 1I, 1J, and 1K; (7) 1H, 1I, 1J, 1L, 1M, and 1N; (8) 1E, 1H, 1I, 1J, 1L, 1M, and 1N; (9) 1F, 1G, 1H, 1I, 1J, 1L, 1M, and 1N; (10) 1K, 1H, 1I, 1J, 1L, 1M, and 1N; and (11) 1H, 1I, 1J, 1O, and 1P, wherein 1E is a formate reductase, 1F is a formate ligase, a formate transferase, or a formate synthetase, wherein 1G is a formyl-CoA reductase, wherein 1H is a formyltetrahydrofolate synthetase, wherein 1I is a methenyltetrahydrofolate cyclohydrolase, wherein 1J is a methylenetetrahydrofolate dehydrogenase, wherein 1K is a formaldehyde-forming enzyme or spontaneous, wherein 1L is a glycine cleavage system, wherein 1M is a serine hydroxymethyltransferase, wherein 1N is a serine deaminase, wherein 1O is a methylenetetrahydrofolate reductase, wherein 1P is an acetyl-CoA synthase, wherein said methanol metabolic pathway comprises a pathway selected from: (12) 10J; (13) 10A, (14) 10A and 10B; (15) 10A, 10B and 10C; (16) 10J, 10K and 10C; (17) 10J, 10M, and 10N; (18) 10J and 10L; (19) 10J, 10L and 10G; (20) 10J, 10L, and 10I; (21) 10A, 10B, 10C, 10D, and 10E; (22) 10A, 10B, 10C, 10D, and 10F; (23) 10J, 10K, 10C, 10D, and 10E; (24) 10J, 10K, 10C, 10D, and 10F; (25) 10J, 10M, 10N, and 10O; (26) 10A, 10B, 10C, 10D, 10E, and 10G; (27) 10A, 10B, 10C, 10D, 10F, and 10G; (28) 10J, 10K, 10C, 10D, 10E, and 10G; (29) 10J, 10K, 10C, 10D, 10F, and 10G; (30) 10J, 10M, 10N, 10O, and 10G; (31) 10A, 10B, 10C, 10D, 10E, and 10I; (32) 10A, 10B, 10C, 10D, 10F, and 10I; (33) 10J, 10K, 10C, 10D, 10E, and 10I; (34) 10J, 10K, 10C, 10D, 10F, and 10I; and (35) 10J, 10M, 10N, 10O, and 10I, wherein 10A is a methanol methyltransferase, wherein 10B is a methylenetetrahydrofolate reductase, wherein 10C is a methylenetetrahydrofolate dehydrogenase, wherein 10D is a methenyltetrahydrofolate cyclohydrolase, wherein 10E is a formyltetrahydrofolate deformylase, wherein 10F is a formyltetrahydrofolate synthetase, wherein 10G is a formate hydrogen lyase, wherein 10I is a formate dehydrogenase, wherein 10J is a methanol dehydrogenase, wherein 10K is a formaldehyde activating enzyme or spontaneous, wherein 10L is a formaldehyde dehydrogenase, wherein 10M is a S-(hydroxymethyl)glutathione synthase or spontaneous, wherein 10N is a glutathione-dependent formaldehyde dehydrogenase, wherein 10O is a S-formylglutathione hydrolase, wherein said FAACPE cycle comprises one or more β-ketoacyl-ACP synthase, one or more β-ketoacyl-ACP reductase, one or more β-hydroxyacyl-ACP reductase, and one or more enoyl ACP-reductase, wherein said termination pathway comprises a pathway selected from: (36) 12I; (37) 12J; (38) 12I, 12K, and 12L; (39) 12I and 12O; (40) 12J and 12M; (41) 12I, 12K, 12L, and 12M; (42) 12I, 12O, and 12M; (43) 12I, 12K and 12N; (44) 12P, wherein 12I is a thioesterase, wherein 12J is a fatty acyl-ACP reductase, wherein 12K is an acyl-CoA synthase, wherein 12L is an acyl-CoA reductase, wherein 12M is a fatty aldehyde reductase, wherein 12N is a fatty alcohol forming acyl-CoA reductase (FAR), wherein 12O is a carboxylic acid reductase (CAR), wherein 12P is an acyl-ACP reductase (alcohol forming), wherein an enzyme of the formaldehyde fixation pathway, the formate assimilation pathway, the methanol metabolic pathway, the FAACPE cycle or the termination pathway is encoded by at least one exogenous nucleic acid and is expressed in a sufficient amount to produce a compound of Formula (I):

wherein R₁ is C₁₋₂₄ linear alkyl; R₂ is CH₂OH, CHO, or COOH; R₃ is H, OH, or oxo (═O); and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four, wherein the substrate of each of said enzymes of the FAACPE cycle and the termination pathway are independently selected from a compound of Formula (II) or malonyl-ACP:

wherein R₁ is C₁₋₂₄ linear alkyl; R₃ is H, OH, or oxo (═O); R₄ is S-CoA, ACP, OH or H; and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four; wherein said one or more enzymes of the FAACPE cycle are each selective for a compound of Formula (II) having a number of carbon atoms at R₁ that is no greater than the number of carbon atoms at R₁ of said compound of Formula (I), and wherein said one or more enzymes of the termination pathway are each selective for a compound of Formula (II) having a number of carbon atoms at R₁ that is no less than the number of carbon atoms at R₁ of said compound of Formula (I).

In some embodiments, the non-naturally occurring microbial organism of the invention has a combination of one or more pathways for generating substrates, intermediates and/or reducing equivalents that can be used with elongation cycles and termination pathways described herein for producing a fatty alcohol, fatty acid or fatty aldehyde of the invention. Accordingly, in some embodiments, the microbial organism has a formaldehyde fixation pathway and a MI-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formate assimilation pathway and a MI-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, and a MI-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway and a MD-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formate assimilation pathway and a MD-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, and a MD-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a methanol metabolic pathway and a MI-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a methanol metabolic pathway and a MD-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a methanol metabolic pathway and a MI-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formate assimilation pathway, a methanol metabolic pathway and a MI-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, a methanol metabolic pathway and a MI-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a methanol metabolic pathway and a MD-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formate assimilation pathway, a methanol metabolic pathway and a MD-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, a methanol metabolic pathway and MD-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway and an FAACPE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formate assimilation pathway and an FAACPE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, and an FAACPE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a methanol metabolic pathway and an FAACPE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a methanol metabolic pathway and an FAACPE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formate assimilation pathway, a methanol metabolic pathway and an FAACPE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, a methanol metabolic pathway and an FAACPE cycle in combination with a termination pathway.

In some embodiment, the non-naturally occurring microbial organism of the invention having FAACPE cycle in combination with a termination pathway as described herein, can further include a pathway for production of substrants for the FAACPE cycle, such as acetoacetyl-ACP or 3-oxovalery-ACP. Accordingly, in some embodiments, the microbial organism further comprises an acetoacetyl-ACP pathway of: (1) 12A, 12B, and 12C; or (2) 12A, 12B, and 12D, wherein 12A is an acetyl-CoA carboxylase, wherein 12B is malonyl-CoA ACP transacylase, wherein 12C is an acetoacetyl-ACP synthase, and wherein 12D is a β-ketoacyl-ACP synthase. In some embodiments, the microbial organism further comprises a 3-oxovalery-ACP pathway comprising an acetyl-CoA carboxylase, a malonyl-CoA ACP transacylase, and a β-ketoacyl-ACP synthase. In some aspects of the invention, an enzyme of the acetoacetyl-ACP pathway is encoded by at least one exogenous nucleic acid and is expressed in a sufficient amount to produce acetoacetyl-ACP wherein the acetoacetyl-ACP is a β-ketoacyl-ACP of the FAACPE cycle. In some aspects of the invention, an enzyme of the 3-oxovalery-ACP pathway is encoded by at least one exogenous nucleic acid and is expressed in a sufficient amount to produce 3-oxovalery-ACP, wherein the 3-oxovalery-ACP is a β-ketoacyl-ACP of the FAACPE cycle.

In some aspects of the invention, non-naturally occurring microbial organism of the invention can produce a compound of Formula (I) wherein R₁ is C₁₋₁₇ linear alkyl. In another aspect of the invention, the R₁ of the compound of Formula (I) is C₁ linear alkyl, C₂ linear alkyl, C₃ linear alkyl, C₄ linear alkyl, C₅ linear alkyl, C₆ linear alkyl, C₇ linear alkyl, C₈ linear alkyl, C₉ linear alkyl, C₁₀ linear alkyl, C₁₁, linear alkyl, C₁₂ linear alkyl or C₁₃ linear alkyl, C₁₄ linear alkyl, C₁₅ linear alkyl, C₁₆ linear alkyl, C₁₇ linear alkyl, C₁₈ linear alkyl, C₁₉ linear alkyl, C₂₀ linear alkyl, C₂₁ linear alkyl, C₂₂ linear alkyl, C₂₃ linear alkyl, or C₂₄ linear alkyl.

In some aspects of the invention, the microbial organism includes two, three, or four exogenous nucleic acids each encoding an enzyme of the MI-FAE cycle, the MD-FAE cycle, or the FAACPE cycle. In some aspects of the invention, the microbial organism includes two, three, or four exogenous nucleic acids each encoding an enzyme of the termination pathway. In some aspects of the invention, the microbial organism includes one, two, three, four, five, six, seven, or eight exogenous nucleic acids each encoding a formaldehyde fixation pathway enzyme, a formate assimilation pathway enzyme, or a methanol metabolic pathway enzyme. In some aspects of the invention, the microbial organism includes exogenous nucleic acids encoding each of the enzymes of at least one of the pathways selected from (1)-(49) for a microbial organism having a MI-FAE cycle or a MD-FAE cycle in combination with a termination pathway as depicted in FIGS. 1, 2, 7, 8 and 10. In some aspects of the invention, the microbial organism includes exogenous nucleic acids encoding each of the enzymes of at least one of the pathways selected from (1)-(44) for a microbial organism having a fatty acyl-ACP elongation (FAACPE) cycle in combination with a termination pathway as depicted in FIGS. 1, 10 and 12.

In some embodiments, the invention provides a non naturally occurring microbial organism, wherein the one or more enzymes of the MI-FAE cycle, MD-FAE cycle, FAACPE cycle or termination pathway is expressed in a sufficient amount to produce a fatty alcohol selected from the Formulas (III)-(VI):

wherein R₁ is C₁₋₂₄ linear alkyl, or alternatively R₁ is C₁₋₁₇ linear alkyl, or alternatively R₁ is C₉₋₁₃ linear alkyl. In some aspects of the invention, R₁ is C₁ linear alkyl, C₂ linear alkyl, C₃ linear alkyl, C₄ linear alkyl, C₅ linear alkyl, C₆ linear alkyl, C₇ linear alkyl, C₈ linear alkyl, C₉ linear alkyl, C₁₀ linear alkyl, C₁₁, linear alkyl, C₁₂ linear alkyl, C₁₃ linear alkyl, C₁₄ linear alkyl, C₁₅ linear alkyl, C₁₆ linear alkyl, C₁₇ linear alkyl, C₁₈ linear alkyl, C₁₉ linear alkyl, C₂₀ linear alkyl, C₂₁ linear alkyl, C₂₂ linear alkyl, C₂₃ linear alkyl, or C₂₄ linear alkyl.

In some embodiments, the invention provides a non naturally occurring microbial organism, wherein the one or more enzymes of the MI-FAE cycle, MD-FAE cycle, FAACPE cycle or termination pathway is expressed in a sufficient amount to produce a fatty aldehyde selected from the Formula (VII)-(X):

wherein R₁ is C₁₋₂₄ linear alkyl, or alternatively R₁ is C₁₋₁₇ linear alkyl, or alternatively R₁ is C₉₋₁₃ linear alkyl. In some aspects of the invention, R₁ is C₁ linear alkyl, C₂ linear alkyl, C₃ linear alkyl, C₄ linear alkyl, C₅ linear alkyl, C₆ linear alkyl, C₇ linear alkyl, C₈ linear alkyl, C₉ linear alkyl, C₁₀ linear alkyl, C₁₁, linear alkyl, C₁₂ linear alkyl, C₁₃ linear alkyl, C₁₄ linear alkyl, C₁₅ linear alkyl, C₁₆ linear alkyl, C₁₇ linear alkyl, C₁₈ linear alkyl, C₁₉ linear alkyl, C₂₀ linear alkyl, C₂₁ linear alkyl, C₂₂ linear alkyl, C₂₃ linear alkyl, or C₂₄ linear alkyl.

In some embodiments, the invention provides a non naturally occurring microbial organism, wherein the one or more enzymes of the MI-FAE cycle, MD-FAE cycle, FAACPE cycle or termination pathway is expressed in a sufficient amount to produce a fatty acid selected from the Formula (XI)-(XIV):

wherein R₁ is C₁₋₂₄ linear alkyl, or alternatively R₁ is C₁₋₁₇ linear alkyl, or alternatively R₁ is C₉₋₁₃ linear alkyl. In some aspects of the invention, R₁ is C₁ linear alkyl, C₂ linear alkyl, C₃ linear alkyl, C₄ linear alkyl, C₅ linear alkyl, C₆ linear alkyl, C₇ linear alkyl, C₈ linear alkyl, C₉ linear alkyl, C₁₀ linear alkyl, C₁₁, linear alkyl, C₁₂ linear alkyl, C₁₃ linear alkyl, C₁₄ linear alkyl, C₁₅ linear alkyl, C₁₆ linear alkyl, C₁₇ linear alkyl, C₁₈ linear alkyl, C₁₉ linear alkyl, C₂₀ linear alkyl, C₂₁ linear alkyl, C₂₂ linear alkyl, C₂₃ linear alkyl, or C₂₄ linear alkyl.

In some embodiments, the invention provides a non naturally occurring microbial organism, wherein one or more enzymes of the MI-FAE cycle and/or MD-FAE cycle are each selective for a compound of Formula (II) wherein R₁ is C₁₋₂₄ linear alkyl, or alternatively R₁ is C₁₋₁₇ linear alkyl, or alternatively R₁ is C₉₋₁₃ linear alkyl. In some aspects of the invention, R₁ is C₁ linear alkyl, C₂ linear alkyl, C₃ linear alkyl, C₄ linear alkyl, C₅ linear alkyl, C₆ linear alkyl, C₇ linear alkyl, C₈ linear alkyl, C₉ linear alkyl, C₁₀ linear alkyl, C₁₁, linear alkyl, C₁₂ linear alkyl, C₁₃ linear alkyl, C₁₄ linear alkyl, C₁₅ linear alkyl, C₁₆ linear alkyl, C₁₇ linear alkyl, C₁₈ linear alkyl, C₁₉ linear alkyl, C₂₀ linear alkyl, C₂₁ linear alkyl, C₂₂ linear alkyl, C₂₃ linear alkyl, or C₂₄ linear alkyl.

In some embodiments, the invention provides a non naturally occurring microbial organism, wherein one or more enzymes of the FAACPE cycle are each selective for a compound of Formula (II) wherein R₁ is C₁₋₂₄ linear alkyl, or alternatively R₁ is C₁₋₁₇ linear alkyl, or alternatively R₁ is C₉₋₁₃ linear alkyl. In some aspects of the invention, R₁ is C₁ linear alkyl, C₂ linear alkyl, C₃ linear alkyl, C₄ linear alkyl, C₅ linear alkyl, C₆ linear alkyl, C₇ linear alkyl, C₈ linear alkyl, C₉ linear alkyl, C₁₀ linear alkyl, C₁₁, linear alkyl, C₁₂ linear alkyl, C₁₃ linear alkyl, C₁₄ linear alkyl, C₁₅ linear alkyl, C₁₆ linear alkyl, C₁₇ linear alkyl, C₁₈ linear alkyl, C₁₉ linear alkyl, C₂₀ linear alkyl, C₂₁ linear alkyl, C₂₂ linear alkyl, C₂₃ linear alkyl, or C₂₄ linear alkyl.

In some embodiments, the invention provides a non naturally occurring microbial organism, wherein one or more enzymes of the termination pathway are each selective for a compound of Formula (II) wherein R₁ is C₁₋₂₄ linear alkyl, or alternatively R₁ is C₁₋₁₇ linear alkyl, or alternatively R₁ is C₉₋₁₃ linear alkyl. In some aspects of the invention, R₁ is C₁ linear alkyl, C₂ linear alkyl, C₃ linear alkyl, C₄ linear alkyl, C₅ linear alkyl, C₆ linear alkyl, C₇ linear alkyl, C₈ linear alkyl, C₉ linear alkyl, C₁₀ linear alkyl, C₁₁, linear alkyl, C₁₂ linear alkyl, C₁₃ linear alkyl, C₁₄ linear alkyl, C₁₅ linear alkyl, C₁₆ linear alkyl, C₁₇ linear alkyl, C₁₈ linear alkyl, C₁₉ linear alkyl, C₂₀ linear alkyl, C₂₁ linear alkyl, C₂₂ linear alkyl, C₂₃ linear alkyl, or C₂₄ linear alkyl.

In some embodiments, the invention provides a non-naturally occurring microbial organism having: (i) a formaldehyde fixation pathway; (ii) a formate assimilation pathway; and/or (iii) a methanol metabolic pathway as depicted in FIGS. 1 and 10, and an isopropanol pathway as depicted in FIG. 11, wherein said formaldehyde fixation pathway comprises. (1) 1B and 1C; (2) 1D; or (3) 1D and 1Z, wherein 1B is a 3-hexulose-6-phosphate synthase, wherein 1C is a 6-phospho-3-hexuloisomerase, wherein 1D is a dihydroxyacetone synthase, wherein 1Z is a fructose-6-phosphate aldolase, wherein said formate assimilation pathway comprises a pathway selected from: (4) 1E; (5) 1F, and 1G; (6) 1H, 1I, 1J, and 1K; (7) 1H, 1I, 1J, 1L, 1M, and 1N; (8) 1E, 1H, 1I, 1J, 1L, 1M, and 1N; (9) 1F, 1G, 1H, 1I, 1J, 1L, 1M, and 1N; (10) 1K, 1H, 1I, 1J, 1L, 1M, and 1N; and (11) 1H, 1I, 1J, 1O, and 1P, wherein 1E is a formate reductase, 1F is a formate ligase, a formate transferase, or a formate synthetase, wherein 1G is a formyl-CoA reductase, wherein 1H is a formyltetrahydrofolate synthetase, wherein 1I is a methenyltetrahydrofolate cyclohydrolase, wherein 1J is a methylenetetrahydrofolate dehydrogenase, wherein 1K is a formaldehyde-forming enzyme or spontaneous, wherein 1L is a glycine cleavage system, wherein 1M is a serine hydroxymethyltransferase, wherein 1N is a serine deaminase, wherein 1O is a methylenetetrahydrofolate reductase, wherein 1P is an acetyl-CoA synthase, wherein said methanol metabolic pathway comprises a pathway selected from: (12) 10J; (13) 10A, (14) 10A and 10B; (15) 10A, 10B and 10C; (16) 10J, 10K and 10C; (17) 10J, 10M, and 10N; (18) 10J and 10L; (19) 10J, 10L and 10G; (20) 10J, 10L, and 10I; (21) 10A, 10B, 10C, 10D, and 10E; (22) 10A, 10B, 10C, 10D, and 10F; (23) 10J, 10K, 10C, 10D, and 10E; (24) 10J, 10K, 10C, 10D, and 10F; (25) 10J, 10M, 10N, and 10O; (26) 10A, 10B, 10C, 10D, 10E, and 10G; (27) 10A, 10B, 10C, 10D, 10F, and 10G; (28) 10J, 10K, 10C, 10D, 10E, and 10G; (29) 10J, 10K, 10C, 10D, 10F, and 10G; (30) 10J, 10M, 10N, 10O, and 10G; (31) 10A, 10B, 10C, 10D, 10E, and 10I; (32) 10A, 10B, 10C, 10D, 10F, and 10I; (33) 10J, 10K, 10C, 10D, 10E, and 10I; (34) 10J, 10K, 10C, 10D, 10F, and 10I; and (35) 10J, 10M, 10N, 10O, and 10I, wherein 10A is a methanol methyltransferase, wherein 10B is a methylenetetrahydrofolate reductase, wherein 10C is a methylenetetrahydrofolate dehydrogenase, wherein 10D is a methenyltetrahydrofolate cyclohydrolase, wherein 10E is a formyltetrahydrofolate deformylase, wherein 10F is a formyltetrahydrofolate synthetase, wherein 10G is a formate hydrogen lyase, wherein 10I is a formate dehydrogenase, wherein 10J is a methanol dehydrogenase, wherein 10K is a formaldehyde activating enzyme or spontaneous, wherein 10L is a formaldehyde dehydrogenase, wherein 10M is a S-(hydroxymethyl)glutathione synthase or spontaneous, wherein 10N is a glutathione-dependent formaldehyde dehydrogenase, wherein 10O is a S-formylglutathione hydrolase, wherein said isopanol pathway comprises. (36) 11V, 11W, 11X, and 11Y; or (37) 11T, 11U, 11W, 11X, and 11Y, wherein 11T is an acetyl-CoA carboxylase, wherein 11U is an acetoacetyl-CoA synthase, wherein 11V is an acetyl-CoA:acetyl-CoA acyltransferase, wherein 11W is an acetoacetyl-CoA hydrolase, an acetoacetyl-CoA transferase, an acetoacetyl-CoA ligase, or a phosphotransacetoacetylase/acetoacetate kinase, wherein 11X is an acetoacetate decarboxylase, wherein 11Y is an acetone reductase or isopropanol dehydrogenase, wherein an enzyme of the formaldehyde fixation pathway, formate assimilation pathway, methanol metabolic pathway, or isopropanol pathway is encoded by at least one exogenous nucleic acid and is expressed in a sufficient amount to produce isopropanol.

In some embodiments, the non-naturally occurring microbial organism of the invention has a combination of one or more pathways for generating substrates, intermediates and/or reducing equivalents that can be used with isopropanol pathways described herein for producing isopropanol of the invention. Accordingly, in some embodiments, the microbial organism has a formaldehyde fixation pathway and an isopropanol pathway. In some embodiments, the microbial organism has a formate assimilation pathway and an isopropanol pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, and an isopropanol pathway. In some embodiments, the microbial organism has a methanol metabolic pathway and an isopropanol pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a methanol metabolic pathway and an isopropanol pathway. In some embodiments, the microbial organism has a formate assimilation pathway, a methanol metabolic pathway and an isopropanol pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, a methanol metabolic pathway and an isopropanol pathway.

In some aspects of the invention, the microbial organism includes two, three, four, five or six exogenous nucleic acids each encoding an enzyme of the isopropanol pathway. In some aspects of the invention, the microbial organism includes one, two, three, four, five, six, seven, or eight exogenous nucleic acids each encoding a formaldehyde fixation pathway enzyme, a formate assimilation pathway enzyme, or a methanol metabolic pathway enzyme. In some aspects of the invention, the microbial organism includes exogenous nucleic acids encoding each of the enzymes of at least one of the pathways selected from (1)-(37) for a microbial organism having an isopropanol pathway as depicted in FIGS. 1, 10 and 11.

In some embodiments, a non-naturally occurring microbial organism of the invention having a formate assimilation pathway further includes wherein the formate assimilation pathway comprises. (1) 1Q; (2) 1R, and 1 S; (3) 1Y and 1Q; (4) 1Y, 1R and 1S, wherein 1Q is a pyruvate formate lyase, wherein 1R is a pyruvate dehydrogenase, a pyruvate ferredoxin oxidoreductase, or a pyruvate:NADP+ oxidoreductase, wherein 1S is a formate dehydrogenase, wherein 1Y is a glyceraldehydes-3-phosphate dehydrogenase or an enzyme of lower glycolysis. In addition to a glyceraldehyde-3-phosphate dehydrogenase, lower glycolysis includes a phosphoglycerate kinase, a phosphoglyceromutase, an enolase, a pyruvate kinase or a PTS-dependant substrate import. Accordingly, in some embodiments, the formate assimilation pathway comprising 1Y includes an enzyme selected from a phosphoglycerate kinase, a phosphoglyceromutase, an enolase, a pyruvate kinase and a PTS-dependant substrate import.

In some embodiments, a non-naturally occurring microbial organism of the invention includes a methanol oxidation pathway. Such a pathway can include at least one exogenous nucleic acid encoding a methanol oxidation pathway enzyme expressed in a sufficient amount to produce formaldehyde in the presence of methanol. An exemplary methanol oxidation pathway enzyme is a methanol dehydrogenase. Accordingly, in some embodiments, a non-naturally occurring microbial organism of the invention includes at least one exogenous nucleic acid encoding a methanol dehydrogenase expressed in a sufficient amount to produce formaldehyde in the presence of methanol.

In some embodiments, the exogenous nucleic acid encoding an methanol dehydrogenase is expressed in a sufficient amount to produce an amount of formaldehyde greater than or equal to 1 μM, 10 μM, 20 μM, or 50 μM, or a range thereof, in culture medium or intracellularly. In other embodiments, the exogenous nucleic acid encoding an methanol dehydrogenase is capable of producing an amount of formaldehyde greater than or equal to 1 μM, 10 μM, 20 μM, or 50 μM, or a range thereof, in culture medium or intracellularly. In some embodiments, the range is from 1 μM to 50 μM or greater. In other embodiments, the range is from 10 μM to 50 μM or greater. In other embodiments, the range is from 20 μM to 50 μM or greater. In other embodiments, the amount of formaldehyde production is 50 μM or greater. In specific embodiments, the amount of formaldehyde production is in excess of or as compared to, that of a negative control, e.g., the same species of organism that does not comprise the exogenous nucleic acid, such as a wild-type microbial organism or a control microbial organism thereof. In certain embodiments, the methanol dehydrogenase is selected from those provided herein, e.g., as exemplified in Example II (see FIG. 1, Step A, or FIG. 10, Step J). In certain embodiments, the amount of formaldehyde production is determined by a whole cell assay, such as that provided in Example II (see FIG. 1, Step A, or FIG. 10, Step J), or by another assay provided herein or otherwise known in the art. In certain embodiments, formaldehyde utilization activity is absent in the whole cell.

In certain embodiments, the exogenous nucleic acid encoding an methanol dehydrogenase is expressed in a sufficient amount to produce at least 1×, 2×, 3×, 4×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100× or more formaldehyde in culture medium or intracellularly. In other embodiments, the exogenous nucleic acid encoding an methanol dehydrogenase is capable of producing an amount of formaldehyde at least 1×, 2×, 3×, 4×, 5×, 6×, 7×, 8×, 9×, 10×, 15×, 20×, 30×, 40×, 50×, 100×, or a range thereof, in culture medium or intracellularly. In some embodiments, the range is from 1× to 100×. In other embodiments, the range is from 2× to 100×. In other embodiments, the range is from 5× to 100×. In other embodiments, the range is from 10× to 100×. In other embodiments, the range is from 50× to 100×. In some embodiments, the amount of formaldehyde production is at least 20×. In other embodiments, the amount of formaldehyde production is at least 50×. In specific embodiments, the amount of formaldehyde production is in excess of or as compared to, that of a negative control, e.g., the same species of organism that does not comprise the exogenous nucleic acid, such as a wild-type microbial organism or a control microbial organism thereof. In certain embodiments, the methanol dehydrogenase is selected from those provided herein, e.g., as exemplified in Example II (see FIG. 1, Step A, or FIG. 10, Step J). In certain embodiments, the amount of formaldehyde production is determined by a whole cell assay, such as that provided in Example II (see FIG. 1, Step A, or FIG. 10, Step J), or by another assay provided herein or otherwise known in the art. In certain embodiments, formaldehyde utilization activity is absent in the whole cell.

In some embodiments, a non-naturally occurring microbial organism of the invention includes one or more enzymes for generating reducing equivalents. For example, the microbial organism can further include a hydrogenase and/or a carbon monoxide dehydrogenase. In some aspects, the organism comprises an exogenous nucleic acid encoding the hydrogenase or the carbon monoxide dehydrogenase.

A reducing equivalent can also be readily obtained from a glycolysis intermediate by any of several central metabolic reactions including glyceraldehyde-3-phosphate dehydrogenase, pyruvate dehydrogenase, pyruvate formate lyase and NAD(P)-dependant formate dehydrogenase, isocitrate dehydrogenase, alpha-ketoglutarate dehydrogenase, succinate dehydrogenase, and malate dehydrogenase. Additionally, reducing equivalents can be generated from glucose 6-phosphate-1-dehydrogenase and 6-phosphogluconate dehydrogenase of the pentose phosphate pathway. Overall, at most twelve reducing equivalents can be obtained from a C6 glycolysis intermediate (e.g., glucose-6-phosphate, fructose-6-phosphate, fructose-1,6-diphosphate) and at most six reducing equivalents can be generated from a C3 glycolysis intermediate (e.g., dihydroxyacetone phosphate, glyceraldehyde-3-phosphate).

In some embodiments, the at least one exogenous nucleic acid included in the non-naturally occurring microbial organism of the invention is a heterologous nucleic acid. Accordingly, in some embodiments, the at least one exogenous nucleic acid encoding a formaldehyde fixation pathway enzyme described herein is a heterologous nucleic acid. In some embodiments, the at least one exogenous nucleic acid encoding a formate assimilation pathway enzyme described herein is a heterologous nucleic acid. In some embodiments, the at least one exogenous nucleic acid encoding a methanol metabolic pathway enzyme described herein is a heterologous nucleic acid. In some embodiments, the at least one exogenous nucleic acid encoding a MI-FAE cycle enzyme described herein is a heterologous nucleic acid. In some embodiments, the at least one exogenous nucleic acid encoding a MD-FAE cycle enzyme described herein is a heterologous nucleic acid. In some embodiments, the at least one exogenous nucleic acid encoding a FAACPE cycle enzyme described herein is a heterologous nucleic acid. In some embodiments, the at least one exogenous nucleic acid encoding a termination pathway enzyme described herein is a heterologous nucleic acid. In some embodiments, the at least one exogenous nucleic acid encoding an acetoacetyl-ACP pathway enzyme described herein is a heterologous nucleic acid. In some embodiments, the at least one exogenous nucleic acid encoding a 3-oxovalery-ACP pathway enzyme described herein is a heterologous nucleic acid. In some embodiments, the at least one exogenous nucleic acid encoding an isopropanol pathway enzyme described herein is a heterologous nucleic acid. In some embodiments, the at least one exogenous nucleic acid encoding a methanol oxidation pathway enzyme described herein is a heterologous nucleic acid. In some embodiments, the at least one exogenous nucleic acid encoding a hydrogenase or a carbon monoxide dehydrogenase is a heterologous nucleic acid.

In some embodiments, the non-naturally occurring microbial organism of the invention is in a substantially anaerobic culture medium.

In some embodiments, the invention provides a non-naturally occurring microbial organism as described herein, wherein the microbial organism further includes an acetyl-CoA pathway and at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme expressed in a sufficient amount to produce or enhance carbon flux through acetyl-CoA, wherein the acetyl-CoA pathway includes a pathway shown in FIG. 1, 3, 4, 5 or 6 selected from: (1) 3A and 3B; (2) 3A, 3C, and 3D; (3) 3H; (4) 3G and 3D; (5) 3E, 3F and 3B; (6) 3E and 3I; (7) 3J, 3F and 3B; (8) 3J and 3I; (9) 4A, 4B, and 4C; (10) 4A, 4B, 4J, 4K, and 4D; (11) 4A, 4B, 4G, and 4D; (12) 4A, 4F, and 4D; (13) 4N, 4H, 4B and 4C; (14) 4N, 4H, 4B, 4J, 4K, and 4D; (15) 4N, 4H, 4B, 4G, and 4D; (16) 4N, 4H, 4F, and 4D; (17) 4L, 4M, 4B and 4C; (18) 4L, 4M, 4B, 4J, 4K, and 4D; (19) 4L, 4M, 4B, 4G, and 4D; (20) 4L, 4M, 4F, and 4D; (21) 5A, 5B, 5D, 5H, 5I, and 5J; (22) 5A, 5B, 5E, 5F, 5H, 5I, and 5J; (23) 5A, 5B, 5E, 5K, 5L, 5H, 5I, and 5J; (24) 5A, 5C, 5D, 5H, and 5J; (25) 5A, 5C, 5E, 5F, 5H, and 5J; (26) 5A, 5C, 5E, 5K, 5L, 5H, and 5J; (27) 6A, 6B, 6D, and 6G; (28) 6A, 6B, 6E, 6F, and 6G; (29) 6A, 6B, 6E, 6K, 6L, and 6G; (30) 6A, 6C, and 6D; (31) 6A, 6C, 6E, and 6F; (32) 6A, 6C, 6E, 6K, and 6L, (33) 1T and 1V; (34) 1T, 1W, and 1X; (35) 1U and 1V; and (36) 1U, 1W, and 1X, wherein 3A is a pyruvate oxidase (acetate-forming), wherein 3B is an acetyl-CoA synthetase, an acetyl-CoA ligase or an acetyl-CoA transferase, wherein 3C is an acetate kinase, wherein 3D is a phosphotransacetylase, wherein 3E is a pyruvate decarboxylase, wherein 3F is an acetaldehyde dehydrogenase, wherein 3G is a pyruvate oxidase (acetyl-phosphate forming), wherein 3H is a pyruvate dehydrogenase, a pyruvate:ferredoxin oxidoreductase, a pyruvate:NAD(P)H oxidoreductase or a pyruvate formate lyase, wherein 3I is an acetaldehyde dehydrogenase (acylating), wherein 3J is a threonine aldolase, wherein 4A is a phosphoenolpyruvate (PEP) carboxylase or a PEP carboxykinase, wherein 4B is an oxaloacetate decarboxylase, wherein 4C is a malonate semialdehyde dehydrogenase (acetylating), wherein 4D is an acetyl-CoA carboxylase or a malonyl-CoA decarboxylase, wherein 4F is an oxaloacetate dehydrogenase or an oxaloacetate oxidoreductase, wherein 4G is a malonate semialdehyde dehydrogenase (acylating), wherein 4H is a pyruvate carboxylase, wherein 4J is a malonate semialdehyde dehydrogenase, wherein 4K is a malonyl-CoA synthetase or a malonyl-CoA transferase, wherein 4L is a malic enzyme, wherein 4M is a malate dehydrogenase or a malate oxidoreductase, wherein 4N is a pyruvate kinase or a PEP phosphatase, wherein 5A is a citrate synthase, wherein 5B is a citrate transporter, wherein 5C is a citrate/malate transporter, wherein 5D is an ATP citrate lyase, wherein 5E is a citrate lyase, wherein 5F is an acetyl-CoA synthetase or an acetyl-CoA transferase, wherein 5H is a cytosolic malate dehydrogenase, wherein 5I is a malate transporter, wherein 5J is a mitochondrial malate dehydrogenase, wherein 5K is an acetate kinase, wherein 5L is a phosphotransacetylase, wherein 6A is a citrate synthase, wherein 6B is a citrate transporter, wherein 6C is a citrate/oxaloacetate transporter, wherein 6D is an ATP citrate lyase, wherein 6E is a citrate lyase, wherein 6F is an acetyl-CoA synthetase or an acetyl-CoA transferase, wherein 6G is an oxaloacetate transporter, wherein 6K is an acetate kinase, wherein 6L is a phosphotransacetylase, wherein 1T is a fructose-6-phosphate phosphoketolase, wherein 1U is a xylulose-5-phosphate phosphoketolase, wherein 1V is a phosphotransacetylase, wherein 1W is an acetate kinase, wherein 1× is an acetyl-CoA transferase, an acetyl-CoA synthetase, or an acetyl-CoA ligase.

In some aspects, the microbial organism of the invention can include two, three, four, five, six, seven or eight exogenous nucleic acids each encoding an acetyl-CoA pathway enzyme. In some aspects, the microbial organism includes exogenous nucleic acids encoding each of the acetyl-CoA pathway enzymes of at least one of the pathways selected from (1)-(36).

In some embodiments, the invention provides a non-naturally occurring microbial organism as described herein, wherein the microbial organism further includes a propionyl-CoA pathway and at least one exogenous nucleic acid encoding a propionyl-CoA pathway enzyme expressed in a sufficient amount to produce propionyl-CoA, wherein the propionyl-CoA pathway includes a pathway shown in FIG. 22. For example, in some embodiments, the propionyl-CoA pathway comprises a pathway selected from: (1) 22A, 22E, 22F, 22G, 22I, 22J, 22K and 22L; (2) 22A, 22E, 22F, 22G, 22H, 22J, 22K and 22L; (3) 22B, 22E, 22F, 22G, 22I, 22J, 22K and 22L; (4) 22B, 22E, 22F, 22G, 22H, 22J, 22K and 22L; (5) 22C, 22D, 22E, 22F, 22G, 22I, 22J, 22K and 22L; and (6) 22C, 22D, 22E, 22F, 22G, 22H, 22J, 22K and 22L, wherein 22A is a PEP carboxykinase, wherein 22B is a PEP carboxylase, wherein 22C is a Pyruvate kinase, wherein 22D is a Pyruvate carboxylase, wherein 22E is a Malate dehydrogenase, wherein 22F is a Fumarase, wherein 22G is a Fumarate reductase, wherein 22H is a Succinyl-CoA synthetase, wherein 22I is a Succinyl-CoA:3-ketoacid-CoA transferase, wherein 22J is a Methylmalonyl-CoA mutase, wherein 22K is a Methyl-malonyl-CoA epimerase, and wherein 22L is a Methylmalonyl-CoA decarboxylase.

In an additional embodiment, the invention provides a non-naturally occurring microbial organism having a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the non-naturally occurring microbial organism comprises at least one exogenous nucleic acid encoding an enzyme or protein that converts a substrate to a product selected from the group consisting of MeOH to Fald, Fald to H6P, H6P to F6P, Fald to DHA and G3P, DHA and G3P to F6P, F6P to ACTP and E4P, ACTP to ACCOA, ACTP to acetate, acetate to ACCOA, Xu5P to ACTP and G3P, G3P to PYR, PYR to formate and ACCOA, PYR to CO2 and ACCOA, CO2 to formate, formate to Fald, formate to Formyl-CoA, Formyl-CoA to Fald, Formate to FTHF, FTHF to methenyl-THF, methenyl-THF to methylene-THF, methylene-THF to Fald, methylene-THF to glycine, glycine to serine, serine to PYR, methylene-THF to methyl-THF, methyl-THF to ACCOA, two acetyl-CoA molecules to a 3-ketoacyl-CoA, acetyl-CoA plus propionyl-CoA to a ketoacyl-CoA, malonyl-CoA to 3-ketoacyl-CoA, a 3-ketoacyl-CoA to a 3-hydroxyacyl-CoA, a 3-hydroxyacyl-CoA to an enoyl-CoA, an enoyl-CoA to an acyl-CoA, an acyl-CoA plus an acetyl-CoA to a 3-ketoacyl-CoA, an acyl-CoA plus malonyl-CoA to a 3-ketoacyl-CoA, an acyl-CoA to a fatty aldehyde, a fatty aldehyde to a fatty alcohol, an acyl-CoA to a fatty alcohol, an acyl-CoA to an acyl-ACP, an acyl-ACP to a fatty acid, an acyl-CoA to a fatty acid, an acyl-ACP to a fatty aldehyde, a fatty acid to a fatty aldehyde, a fatty aldehyde to a fatty acid, formaldehyde to S-hydroxymethylglutathione, S-hydroxymethylglutathione to S-formylglutathione to formate, formaldehyde to formate, MeOH to methyl-THF, methyl-THF to methylene-THF, formaldehyde to methylene-THF, methylene-THF to methenyl-THF, methenyl-THF to formyl-THF, formyl-THF to formate, formaldehyde to formate, ACCOA to MALCOA, ACCOA to AACOA, MALCOA to AACOA, AACOA to acetoacetate, acetoacetate to acetone, acetone to isopropanol, malonyl-CoA to malonyl-ACP, malonyl-ACP and acetyl-CoA to acetoacetyl-ACP, malonyl-ACP and acetyl-ACP to acetoacetyl-ACP, malonyl-ACP and propionyl-CoA to 3-oxovalery-ACP, malonyl-ACP and an acyl-ACP to a β-ketoacyl-ACP, a β-ketoacyl-ACP to a β-hydroxyacyl-ACP, a β-hydroxyacyl-ACP to a trans-2-enoyl-ACP, a trans-2-enoyl-ACP to an acyl-ACP, an acyl-ACP to a fatty acid, an acyl-ACP to a fatty aldehyde, a fatty acid to a fatty aldehyde, a fatty acid to an acyl-CoA, an acyl-CoA to a fatty aldehyde, a fatty aldehyde to a fatty alcohol, a fatty aldehyde to a fatty alcohol, PEP to OAA, OAA to MAL, MAL to FUM, FUM to SUCC, SUCCOA to (R)-MMCOA, (R)-MMCOA to (S)-MMCOA, MMCOA to PPCOA, PEP to PYR, pyruvate to acetate, acetate to acetyl-CoA, pyruvate to acetyl-CoA, pyruvate to acetaldehyde, threonin to acetaldehyde, acetaldehyde to acetate, acetaldehyde to acetyl-CoA, pyruvate to acetyl-phosphate, acetate to acetyl-phosphate, acetyl-phosphate to acetyl-CoA, phosphoenolpyruvate (PEP) to pyruvate, pyruvate to malate, malate to oxaloacetate, pyruvate to oxaloacetate, PEP to oxaloacetate, oxaloacetate to malonate semialdehyde, oxaloacetate to malonyl-CoA, malonate semialdehyde to malonate, malonate to malonyl-CoA, malonate semialdehyde to malonyl-CoA, malonyl-CoA to acetyl-CoA, malonate semialdehyde to acetyl-CoA, oxaloacetate plus acetyl-CoA to citrate, citrate to oxaloacetate plus acetyl-CoA, citrate to oxaloacetate plus acetate, and oxaloacetate to malate. One skilled in the art will understand that these are merely exemplary and that any of the substrate-product pairs disclosed herein suitable to produce a desired product and for which an appropriate activity is available for the conversion of the substrate to the product can be readily determined by one skilled in the art based on the teachings herein. Thus, the invention provides a non-naturally occurring microbial organism containing at least one exogenous nucleic acid encoding an enzyme or protein, where the enzyme or protein converts the substrates and products of a fatty alcohol, fatty aldehyde, fatty acid, or isopropanol pathway, such as that shown in FIGS. 1-12 and 22.

While generally described herein as a microbial organism that contains a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway, it is understood that the invention additionally provides a non-naturally occurring microbial organism comprising at least one exogenous nucleic acid encoding a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway enzyme or protein expressed in a sufficient amount to produce an intermediate of a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway. For example, as disclosed herein, a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway is exemplified in FIGS. 1-12 and 22. Therefore, in addition to a microbial organism containing a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway that produces fatty alcohol, fatty aldehyde, fatty acid or isopropanol, the invention additionally provides a non-naturally occurring microbial organism comprising at least one exogenous nucleic acid encoding a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway enzyme, where the microbial organism produces a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway intermediate, for example, pyruvate, formate, formaldehyde, H6P, DHA, G3P, F6P, ACTP, E4P, formyl-CoA, FTHF, methenyl-THF, methylene-THF, glycine, serine, methyl-THF, CO2, a 3-ketoacyl-CoA, a 3-hydroxyacyl-CoA, an enoyl-CoA, a β-ketoacyl-ACP, a β-hydroxyacyl-ACP, a trans-2-enoyl-ACP, an acyl-CoA, an acyl-ACP, acetoacetate, acetone, acetate, acetaldehyde, acetyl-phosphate, oxaloacetate, matate, malonate semialdehyde, malonate, malonyl-ACP, propionyl-CoA, malonyl-CoA, acetyl-CoA, or citrate.

It is understood that any of the pathways disclosed herein, as described in the Examples and exemplified in the Figures, including the pathways of FIGS. 1-12 and 22, can be utilized to generate a non-naturally occurring microbial organism that produces any pathway intermediate or product, as desired. As disclosed herein, such a microbial organism that produces an intermediate can be used in combination with another microbial organism expressing downstream pathway enzymes to produce a desired product. However, it is understood that a non-naturally occurring microbial organism that produces a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway intermediate can be utilized to produce the intermediate as a desired product.

In some embodiments, the invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway, wherein said acetyl-CoA pathway comprises a pathway selected from: (1) 1T and 1V; (2) 1T, 1W, and 1X; (3) 1U and 1V; (4) 1U, 1W, and 1X; wherein 1T is a fructose-6-phosphate phosphoketolase, wherein 1U is a xylulose-5-phosphate phosphoketolase, wherein 1V is a phosphotransacetylase, wherein 1W is an acetate kinase, wherein 1X is an acetyl-CoA transferase, an acetyl-CoA synthetase, or an acetyl-CoA ligase, wherein said non-naturally occurring microbial organism further comprises a pathway capable of producing isopropanol and an exogenous nucleic acid encoding an isopropanol pathway enzyme expressed in a sufficient amount to produce isopropanol, wherein said isopropanol pathway comprises a pathway selected from: (1) 11V, 11W, 11X, and 11Y; or (2) 11T, 11U, 11W, 11X, and 11Y, wherein 11T is an acetyl-CoA carboxylase, wherein 11U is an acetoacetyl-CoA synthase, wherein 11V is an acetyl-CoA:acetyl-CoA acyltransferase, wherein 11W is an acetoacetyl-CoA hydrolase, an acetoacetyl-CoA transferase, an acetoacetyl-CoA ligase, or a phosphotransacetoacetylase/acetoacetate kinase, wherein 11X is an acetoacetate decarboxylase, wherein 11Y is an acetone reductase or isopropanol dehydrogenase.

The invention further provides non-naturally occurring microbial organisms that have elevated or enhanced synthesis or yields of acetyl-CoA (e.g. intracellular) or biosynthetic products such as a fatty alcohol, fatty aldehyde, fatty acid or isopropanol and methods of using those non-naturally occurring organisms to produce such biosynthetic products. The enhanced synthesis of intracellular acetyl-CoA enables enhanced production of a fatty alcohol, fatty aldehyde, fatty acid or isopropanol from which acetyl-CoA is an intermediate and further, may have been rate limiting.

The non-naturally occurring microbial organisms having enhanced yields of a biosynthetic product include one or more of the various pathway configurations employing a methanol dehydrogenase for methanol oxidation, a formaldehyde fixation pathway and/or an acetyl-CoA enhancing pathway, e.g. phosphoketolase, for directing the carbon from methanol into acetyl-CoA and other desired products via formaldehyde fixation. The various different methanol oxidation and formaldehyde fixation configurations exemplified below can be engineered in conjunction with any or each of the various methanol oxidation, formaldehyde fixation, formate reutilization, fatty alcohol, fatty aldehyde, fatty acid and/or isopropanol pathways exemplified previously and herein. The metabolic modifications exemplified below increase biosynthetic product yields over, for example, endogenous methanol utilization pathways because they further focus methanol derived carbon into the assimilation pathways described herein, decrease inefficient use of methanol carbon through competing methanol utilization and/or formaldehyde fixation pathways and/or increase the production of reducing equivalents.

In this regard, methylotrophs microbial organisms utilize methanol as the sole source of carbon and energy. In such methylotrophic organisms, the oxidation of methanol to formaldehyde is catalyzed by one of three different enzymes: NADH dependent methanol dehydrogenase (MeDH), PQQ-dependent methanol dehydrogenase (MeDH-PQQ) and alcohol oxidase (AOX). Methanol oxidase is a specific type of AOX with activity on methanol. Gram positive bacterial methylotrophs such as Bacillus methanolicus utilize a cytosolic MeDH which generates reducing equivalents in the form of NADH. Gram negative bacterial methylotrophs utilize periplasmic PQQ-containing methanol dehydrogenase enzymes which transfer electrons from methanol to specialized cytochromes CL, and subsequently to a cytochrome oxidase (Afolabi et al, Biochem 40:9799-9809 (2001)). Eukaryotic methylotrophs employ a peroxisomal oxygen-consuming and hydrogen-peroxide producing alcohol oxidase.

Bacterial methylotrophs are found in the genera Bacillus, Methylobacterium, Methyloversatilis, Methylococcus, Methylocystis and Hyphomicrobium. These organisms utilize either the serine cycle (type II) or the RuMP cycle (type I) to further assimilate formaldehyde into central metabolism (Hanson and Hanson, Microbiol Rev 60:439-471 (1996)). As described previously, the RuMP pathway combines formaldehyde with ribulose monophosphate to form hexulose-6-phosphate, which is further converted to fructose-6-phosphate (see FIG. 1, step C). In the serine cycle formaldehyde is initially converted to 5,10-methylene-THF, which is combined with glycine to form serine. Overall, the reactions of the serine cycle produce one equivalent of acetyl-CoA from three equivalents of methanol (Anthony, Science Prog 94:109-37 (2011)). The RuMP cycle also yields one equivalent of acetyl-CoA from three equivalents methanol in the absence of phosphoketolase activity or a formate assimilation pathway. Genetic tools are available for numerous prokaryotic methylotrophs and methanotrophs.

Eukaryotic methylotrophs are found in the genera Candida, Pichia, Ogataea, Kuraishia and Komagataella. Particularly useful methylotrophic host organisms are those with well-characterized genetic tools and gene expression systems such as Hansenula polymorpha, Pichia pastoris, Candida boidinii and Pichia methanolica (for review see Yurimoto et al, Int J Microbiol (2011)). The initial step of methanol assimilation in eukaryotic methylotrophs occurs in the peroxisomes, where methanol and oxygen are oxidized to formaldehyde and hydrogen peroxide by alcohol oxidase (AOX). Formaldehyde assimilation with xylulose-5-phosphate via DHA synthase also occurs in the peroxisomes. During growth on methanol, the two enzymes DHA synthase and AOX together comprise 80% of the total cell protein (Horiguchi et al, J Bacteriol 183:6372-83 (2001)). DHA synthase products, DHA and glyceraldehyde-3-phosphate, are secreted into the cytosol where they undergo a series of rearrangements catalyzed by pentose phosphate pathway enzymes, and are ultimately converted to cellular constituents and xylulose-5-phosphate, which is transported back into the peroxisomes. The initial step of formaldehyde dissimilation, catalyzed by S-(hydroxymethyl)-glutathione synthase, also occurs in the peroxisomes. Like the bacterial methylotrophic pathways described above, eukaryotic methylotrophic pathways convert three equivalents of methanol to at most one equivalent of acetyl-CoA because they lack phosphoketolase activity or a formate assimilation pathway.

As exemplified further below, the various configurations of metabolic modifications disclosed herein for enhancing product yields via methanol derived carbon include enhancing methanol oxidation and production of reducing equivalents using either an endogenous NADH dependent methanol dehydrogenase, an exogenous NADH dependent methanol dehydrogenase, both an endogenous NADH dependent methanol dehydrogenase and exogenous NADH dependent methanol dehydrogenase alone or in combination with one or more metabolic modifications that attenuate, for example, DHA synthase and/or AOX. In addition, other metabolic modifications as exemplified below that reduce carbon flux away from methanol oxidation and formaldehyde fixation also can be included, alone or in combination, with the methanol oxidation and formaldehyde fixation pathway configurations disclosed herein that enhance carbon flux into product precursors such as acetyl-CoA and, therefore, enhance product yields.

Accordingly, the microbial organisms of the invention having one or more of any of the above and/or below metabolic modifications to a methanol utilization pathway and/or formaldehyde assimilation pathway configurations for enhancing product yields can be combined with any one or more, including all of the previously described methanol oxidation, formaldehyde fixation, formate reutilization, fatty alcohol, fatty aldehyde, acid and/or isopropanol pathways to enhance the yield and/or production of a product such as any of the fatty alcohol, fatty aldehyde, fatty acids and/or isopropanol described herein.

Given the teachings and guidance provided herein, the methanol oxidation and formaldehyde fixation pathway configurations can be equally engineered into both prokaryotic and eukaryotic organisms. In prokaryotic microbial organisms, for example, one skilled in the art will understand that utilization of an endogenous methanol oxidation pathway enzyme or expression of an exogenous nucleic acid encoding a methanol oxidation pathway enzyme will naturally occur cytosolically because prokaryotic organisms lack peroxisomes. In eukaryotic microbial organisms one skilled in the art will understand that certain methanol oxidation pathways occur in the peroxisome as described above and that cytosolic expression of the methanol oxidation pathway or pathways described herein to enhance product yields can be beneficial. The peroxisome located pathways and competing pathways remain or, alternatively, attenuated as described below to further enhance methanol oxidation and formaldehyde fixation.

With respect to eukaryotic microbial host organisms, those skilled in the art will know that yeasts and other eukaryotic microorganisms exhibit certain characteristics distinct from prokaryotic microbial organisms. When such characteristics are desirable, one skilled in the art can choose to use such eukaryotic microbial organisms as a host for engineering the various different methanol oxidation and formaldehyde fixation configurations exemplified herein for enhancing product yields. For example, yeast are robust organisms, able to grow over a wide pH range and able to tolerate more impurities in the feedstock Yeast also ferment under low growth conditions and are not susceptible to infection by phage. Less stringent aseptic design requirements can also reduce production costs. Cell removal, disposal and propagation are also cheaper, with the added potential for by-product value for animal feed applications. The potential for cell recycle and semi-continuous fermentation offers benefits in increased overall yields and rates. Other benefits include: potential for extended fermentation times under low growth conditions, lower viscosity broth (vs E. coli) with insoluble hydrophobic products, the ability to employ large fermenters with external loop heat exchangers.

Eukaryotic host microbial organisms suitable for engineering carbon efficient methanol utilization capability can be selected from, and the non-naturally occurring microbial organisms generated in, for example, yeast, fungus or any of a variety of other microorganisms applicable to fermentation processes. As described previously, exemplary yeasts or fungi include species selected from the genera Saccharomyces, Schizosaccharomyces, Schizochytrium, Rhodotorula, Thraustochytrium, Aspergillus, Kluyveromyces, Issatchenkia, Yarrowia, Candida, Pichia, Ogataea, Kuraishia, Hansenula and Komagataella. Useful host organisms include Saccharomyces cerevisiae, Schizosaccharomyces pombe, Hansenula polymorpha, Pichia methanolica, Candida boidinii, Kluyveromyces lactis, Kluyveromyces marxianus, Aspergillus terreus, Aspergillus niger, Pichia pastoris, Rhizopus arrhizus, Rhizopus oryzae, Yarrowia lipolytica, Issatchenkia orientalis and the like.

The methanol oxidation and/or formaldehyde assimilation pathway configurations described herein for enhancing product yields include, for example, a NADH-dependent methanol dehydrogenase (MeDH), one or more formaldehyde assimilation pathways and/or one or more phosphoketolases. Such engineered pathways provide a yield advantage over endogenous pathways found in methylotrophic organisms. For example, methanol assimilation via methanol dehydrogenase provides reducing equivalents in the useful form of NADH, whereas alcohol oxidase and PQQ-dependent methanol dehydrogenase do not. Several product pathways described herein have several NADH-dependant enzymatic steps. In addition, deletion of redox-inefficient methanol oxidation enzymes as described further below, combined with increased cytosolic or peroxisomal expression of an NADH-dependent methanol dehydrogenase, improves the ability of the organism to extract useful reducing equivalents from methanol. In some aspects, if NADH-dependent methanol dehydrogenase is engineered into the peroxisome, an efficient means of shuttling redox in the form of NADH out of the peroxisome and into the cytosol can be included. Further employment of a formaldehyde assimilation pathway in combination with a phosphoketolase or formate assimilation pathway enables high yield conversion of methanol to acetyl-CoA, and subsequently to acetyl-CoA derived products.

For example, in a eukaryotic organism such as Pichia pastoris, deleting the endogenous alcohol oxidase and peroxisomal formaldehyde assimilation and dissimilation pathways, and expressing redox and carbon-efficient cytosolic methanol utilization pathways significantly improves the yield of dodecanol, an acetyl-CoA derived product. The maximum docidecanol yield of Pichia pastoris from methanol using endogenous methanol oxidase and formaldehyde assimilation enzymes is 0.256 g dodecanol/g methanol. Adding one or more heterologous cytosolic phosphoketolase enzymes, in combination with a formaldehyde assimilation pathway such as the DHA pathway or the RUMP pathway, boosts the dodecanol yield to 0.306 g dodecanol/g methanol. Deletion of peroxisomal methanol oxidase and formaldehyde assimilation pathway enzymes (alcohol oxidase, DHA synthase), and replacement with cytosolic methanol dehydrogenase (NADH dependent) and formaldehyde assimilation pathways, together with a phosphoketolase, provides a significant boost of yield to 0.422 g/g.

Strain design Max FA yield (assumes DHA pathway) (g dodecanol/g MeOH) Pichia + AOX + fatty acid pathway 0.256 Pichia + AOX + PK 0.306 Pichia + MeDH + PK 0.422

Metabolic modifications for enabling redox- and carbon-efficient cytosolic methanol utilization in a eukaryotic or prokaryotic organism are exemplified in further detail below.

In one embodiment, the invention provides cytosolic expression of one or more methanol oxidation and/or formaldehyde assimilation pathways Engineering into a host microbial organism carbon- and redox-efficient cytosolic formaldehyde assimilation can be achieved by expression of one or more endogenous or exogenous methanol oxidation pathways and/or one or more endogenous or exogenous formaldehyde assimilation pathway enzymes in the cytosol. An exemplary pathway for methanol oxidation includes NADH dependent methanol dehydrogenase as shown in FIG. 1. Exemplary pathways for converting cytosolic formaldehyde into glycolytic intermediates also are shown in FIG. 1. Such pathways include methanol oxidation via expression of an cytosolic NADH dependent methanol dehydrogenase, formaldehyde fixation via expression of cytosolic DHA synthase, both methanol oxidation via expression of an cytosolic NADH dependent methanol dehydrogenase and formaldehyde fixation via expression of cytosolic DHA synthase alone or together with the metabolic modifications exemplified below that attenuate less beneficial methanol oxidation and/or formaldehyde fixation pathways. Such attenuating metabolic modifications include, for example, attenuation of alcohol oxidase, attenuation of DHA kinase and/or when utilization of ribulose-5-phosphate (Ru5P) pathway for formaldehyde fixation attenuation of DHA synthase.

For example, in the carbon-efficient DHA pathway of formaldehyde assimilation shown in FIG. 1, step D, formaldehyde is converted to dihydroxyacetone (DHA) and glyceraldehyde-3-phosphate (GAP) by DHA synthase (FIG. 1D). DHA and G3P are then converted to fructose-6-phosphate in one step by F6P aldolase (FIG. 1C) or in three steps by DHA kinase, FBP aldolase and fructose-1,6-bisphosphatase (not shown). Formation of F6P from DHA and G3P by F6P aldolase is more ATP-efficient than using DHA kinase, FBP aldolase and fructose-1,6-bisphosphatase. Rearrangement of F6P and E4P by enzymes of the pentose phosphate pathway (transaldolase, transketolase, R5P epimerase and Ru5P epimerase) regenerates xylulose-5-phosphate, the DHA synthase substrate. Conversion of F6P to acetyl-phosphate and E4P (FIG. 1T), or Xu5P to G3P and acetyl-phosphate (FIGS. 1T and 1U) by one or more phosphoketolase enzymes results in the carbon-efficient generation of cytosolic acetyl-CoA. Exemplary enzymes catalyzing each step of the carbon efficient DHA pathway are described elsewhere herein.

An alternate carbon efficient pathway for formaldehyde assimilation proceeding through ribulose-5-phosphate (Ru5P) is shown in FIG. 1, step B. The formaldehyde assimilation enzyme of this pathway is 3-hexulose-6-phosphate synthase, which combines ru5p and formaldehyde to form hexulose-6-phosphate (FIG. 1B). 6-Phospho-3-hexuloisomerase converts H6P to F6P (FIG. 1C). Regeneration of Ru5P from F6P proceeds by pentose phosphate pathway enzymes. Carbon-efficient phosphoketolase enzymes catalyze the conversion of F6P and/or Xu5P to acetyl-phosphate and pentose phosphate intermediates. Exemplary enzymes catalyzing each step of the carbon efficient RUMP pathway are described elsewhere herein.

Thus, in this embodiment, conversion of cytosolic formaldehyde into glycolytic intermediates can occur via expression of a cytosolic 3-hexulose-6-phosphate (3-Hu6P) synthase and 6-phospho-3-hexuloisomerase. Thus, exemplary pathways that can be engineered into a microbial organism of the invention can include methanol oxidation via expression of a cytosolic NADH dependent methanol dehydrogenase, formaldehyde fixation via expression of cytosolic 3-Hu6P synthase and 6-phospho-3-hexuloisomerase, both methanol oxidation via expression of an cytosolic NADH dependent dehydrogenase and formaldehyde fixation via expression of cytosolic 3-Hu6P synthase and 6-phospho-3-hexuloisomerase alone or together with the metabolic modifications exemplified below that attenuate less beneficial methanol oxidation and/or formaldehyde fixation pathways. Such attenuating metabolic modifications include, for example, attenuation of alcohol oxidase, attenuation of DHA kinase and/or when utilization of ribulose-5-phosphate (Ru5P) pathway for formaldehyde fixation attenuation of DHA synthase.

In yet another embodiment increased product yields can be accomplished by engineering into the host microbial organism of the invention both the RUMP and DHA pathways as shown in FIG. 1. In this embodiment, the microbial organisms can have cytosolic expression of one or more methanol oxidation and/or formaldehyde assimilation pathways. The formaldehyde assimilation pathways can include both assimilation through cytosolic DHA synthase and 3-Hu6P synthase. Such pathways include methanol oxidation via expression of a cytosolic NADH dependent methanol dehydrogenase, formaldehyde fixation via expression of cytosolic DHA synthase and 3-Hu6P synthase, both methanol oxidation via expression of an cytosolic NADH dependent dehydrogenase and formaldehyde fixation via expression of cytosolic DHA synthase and 3-Hu6P synthase alone or together with the metabolic modifications exemplified previously and also below that attenuate less beneficial methanol oxidation and/or formaldehyde fixation pathways. Such attenuating metabolic modifications include, for example, attenuation of alcohol oxidase, attenuation of DHA kinase and/or attenuation of DHA synthase (e.g. when ribulose-5-phosphate (Ru5P) pathway for formaldehyde fixation is utilized).

Increasing the expression and/or activity of one or more formaldehyde assimilation pathway enzymes in the cytosol can be utilized to assimilate formaldehyde at a high rate. Increased activity can be achieved by increased expression, altering the ribosome binding site, altering the enzyme activity, or altering the sequence of the gene to ensure, for example, that codon usage is balanced with the needs of the host organism, or that the enzyme is targeted to the cytosol as disclosed herein.

In some embodiments, the invention provides a non-naturally occurring microbial organism as described herein, wherein the microbial organism further includes attenuation of one or more endogenous enzymes, which enhances carbon flux through acetyl-CoA. For example, in some aspects, the endogenous enzyme can be selected from DHA kinase, methanol oxidase, PQQ-dependent methanol dehydrogenase, DHA synthase or any combination thereof. Accordingly, in some aspects, the attenuation is of the endogenous enzyme DHA kinase. In some aspects, the attenuation is of the endogenous enzyme methanol oxidase. In some aspects, the attenuation is of the endogenous enzyme PQQ-dependent methanol dehydrogenase. In some aspects, the attenuation is of the endogenous enzyme DHA synthase. The invention also provides a microbial organism wherein attenuation is of any combination of two or three endogenous enzymes described herein. For example, a microbial organism of the invention can include attenuation of DHA kinase and DHA synthase, or alternatively methanol oxidase and PQQ-dependent methanol dehydrogenase, or alternatively DHA kinase, methanol oxidase, and PQQ-dependent methanol dehydrogenase, or alternatively DHA kinase, methanol oxidase, and DHA synthase. The invention also provides a microbial organism wherein attenuation is of all endogenous enzymes described herein. For example, in some aspects, a microbial organism described herein includes attenuation of DHA kinase, methanol oxidase, PQQ-dependent methanol dehydrogenase and DHA synthase.

In some embodiments, the invention provides a non-naturally occurring microbial organism as described herein, wherein the microbial organism further includes attenuation of one or more endogenous enzymes of a competing formaldehyde assimilation or dissimilation pathway. Examples of these endogenous enzymes are disclosed in FIG. 1 and described in Example XXIII. It is understood that a person skilled in the art would be able to readily identify enzymes of such competing pathways. Competing pathways can be dependent upon the host microbial organism and/or the exogenous nucleic acid introduced into the microbial organism as described herein. Accordingly, in some aspects of the invention, the microbial organism includes attenuation of one, two, three, four, five, six, seven, eight, nine, ten or more endogenous enzymes of a competing formaldehyde assimilation or dissimilation pathway.

In some embodiments, the invention provides a non-naturally occurring microbial organism as described herein, wherein the microbial organism further includes a gene disruption of one or more endogenous nucleic acids encoding enzymes, which enhances carbon flux through acetyl-CoA. For example, in some aspects, the endogenous enzyme can be selected from DHA kinase, methanol oxidase, PQQ-dependent methanol dehydrogenase, DHA synthase or any combination thereof. According, in some aspects, the gene disruption is of an endogenous nucleic acid encoding the enzyme DHA kinase. In some aspects, the gene disruption is of an endogenous nucleic acid encoding the enzyme methanol oxidase. In some aspects, the gene disruption is of an endogenous nucleic acid encoding the enzyme PQQ-dependent methanol dehydrogenase. In some aspects, the gene disruption is of an endogenous nucleic acid encoding the enzyme DHA synthase. The invention also provides a microbial organism wherein the gene disruption is of any combination of two or three nucleic acids encoding endogenous enzymes described herein. For example, a microbial organism of the invention can include a gene disruption of DHA kinase and DHA synthase, or alternatively methanol oxidase and PQQ-dependent methanol dehydrogenase, or alternatively DHA kinase, methanol oxidase, and PQQ-dependent methanol dehydrogenase, or alternatively DHA kinase, methanol oxidase, and DHA synthase. The invention also provides a microbial organism wherein all endogenous nucleic acids encoding enzymes described herein are disrupted. For example, in some aspects, a microbial organism described herein includes disruption of DHA kinase, methanol oxidase, PQQ-dependent methanol dehydrogenase and DHA synthase.

In some embodiments, the invention provides a non-naturally occurring microbial organism as described herein, wherein the microbial organism further includes a gene disruption of one or more endogenous enzymes of a competing formaldehyde assimilation or dissimilation pathway. Examples of these endogenous enzymes are disclosed in FIG. 1 and described in Example XXIII. It is understood that a person skilled in the art would be able to readily identify enzymes of such competing pathways. Competing pathways can be dependent upon the host microbial organism and/or the exogenous nucleic acid introduced into the microbial organism as described herein. Accordingly, in some aspects of the invention, the microbial organism includes a gene disruption of one, two, three, four, five, six, seven, eight, nine, ten or more endogenous nucleic acids encoding enzymes of a competing formaldehyde assimilation or dissimilation pathway.

The invention is described herein with general reference to the metabolic reaction, reactant or product thereof, or with specific reference to one or more nucleic acids or genes encoding an enzyme associated with or catalyzing, or a protein associated with, the referenced metabolic reaction, reactant or product. Unless otherwise expressly stated herein, those skilled in the art will understand that reference to a reaction also constitutes reference to the reactants and products of the reaction. Similarly, unless otherwise expressly stated herein, reference to a reactant or product also references the reaction, and reference to any of these metabolic constituents also references the gene or genes encoding the enzymes that catalyze or proteins involved in the referenced reaction, reactant or product. Likewise, given the well known fields of metabolic biochemistry, enzymology and genomics, reference herein to a gene or encoding nucleic acid also constitutes a reference to the corresponding encoded enzyme and the reaction it catalyzes or a protein associated with the reaction as well as the reactants and products of the reaction.

The non-naturally occurring microbial organisms of the invention can be produced by introducing expressible nucleic acids encoding one or more of the enzymes or proteins participating in one or more fatty alcohol, fatty aldehyde, fatty acid or isopropanol biosynthetic pathways. Depending on the host microbial organism chosen for biosynthesis, nucleic acids for some or all of a particular fatty alcohol, fatty aldehyde, fatty acid or isopropanol biosynthetic pathway can be expressed. For example, if a chosen host is deficient in one or more enzymes or proteins for a desired biosynthetic pathway, then expressible nucleic acids for the deficient enzyme(s) or protein(s) are introduced into the host for subsequent exogenous expression. Alternatively, if the chosen host exhibits endogenous expression of some pathway genes, but is deficient in others, then an encoding nucleic acid is needed for the deficient enzyme(s) or protein(s) to achieve fatty alcohol, fatty aldehyde, fatty acid or isopropanol biosynthesis. Thus, a non-naturally occurring microbial organism of the invention can be produced by introducing exogenous enzyme or protein activities to obtain a desired biosynthetic pathway or a desired biosynthetic pathway can be obtained by introducing one or more exogenous enzyme or protein activities that, together with one or more endogenous enzymes or proteins, produces a desired product such as fatty alcohol, fatty aldehyde, fatty acid or isopropanol.

Host microbial organisms can be selected from, and the non-naturally occurring microbial organisms generated in, for example, bacteria, yeast, fungus or any of a variety of other microorganisms applicable or suitable to fermentation processes. Exemplary bacteria include any species selected from the order Enterobacteriales, family Enterobacteriaceae, including the genera Escherichia and Klebsiella; the order Aeromonadales, family Succinivibrionaceae, including the genus Anaerobiospirillum; the order Pasteurellales, family Pasteurellaceae, including the genera Actinobacillus and Mannheimia; the order Rhizobiales, family Bradyrhizobiaceae, including the genus Rhizobium; the order Bacillales, family Bacillaceae, including the genus Bacillus; the order Actinomycetales, families Colynebacteriaceae and Streptomycetaceae, including the genus Corynebacterium and the genus Streptomyces, respectively; order Rhodospirillales, family Acetobacteraceae, including the genus Gluconobacter; the order Sphingomonadales, family Sphingomonadaceae, including the genus Zymomonas; the order Lactobacillales, families Lactobacillaceae and Streptococcaceae, including the genus Lactobacillus and the genus Lactococcus, respectively; the order Clostridiales, family Clostridiaceae, genus Clostridium; and the order Pseudomonadales, family Pseudomonadaceae, including the genus Pseudomonas. Non-limiting species of host bacteria include Escherichia coli, Klebsiella oxytoca, Anaerobiospirillum succiniciproducens, Actinobacillus succinogenes, Mannheimia succiniciproducens, Rhizobium etli, Bacillus subtilis, Corynebacterium glutamicum, Gluconobacter oxydans, Zymomonas mobilis, Lactococcus lactis, Lactobacillus plantarum, Streptomyces coelicolor, Clostridium acetobutylicum, Pseudomonas fluorescens, and Pseudomonas putida. Exemplary bacterial methylotrophs include, for example, Bacillus, Methylobacterium, Methyloversatilis, Methylococcus, Methylocystis and Hyphomicrobium.

Similarly, exemplary species of yeast or fungi species include any species selected from the order Saccharomycetales, family Saccaromycetaceae, including the genera Saccharomyces, Kluyveromyces and Pichia; the order Saccharomycetales, family Dipodascaceae, including the genus Yarrowia; the order Schizosaccharomycetales, family Schizosaccaromycetaceae, including the genus Schizosaccharomyces; the order Eurotiales, family Trichocomaceae, including the genus Aspergillus; and the order Mucorales, family Mucoraceae, including the genus Rhizopus. Non-limiting species of host yeast or fungi include Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces lactis, Kluyveromyces marxianus, Aspergillus terreus, Aspergillus niger, Pichia pastoris, Rhizopus arrhizus, Rhizopus oryzae, Yarrowia lipolytica, and the like. E. coli is a particularly useful host organism since it is a well characterized microbial organism suitable for genetic engineering. Other particularly useful host organisms include yeast such as Saccharomyces cerevisiae and yeasts or fungi selected from the genera Saccharomyces, Schizosaccharomyces, Schizochytrium, Rhodotorula, Thraustochytrium, Aspergillus, Kluyveromyces, Issatchenkia, Yarrowia, Candida, Pichia, Ogataea, Kuraishia, Hansenula and Komagataella. Useful host organisms include Saccharomyces cerevisiae, Schizosaccharomyces pombe, Hansenula polymoipha, Pichia methanolica, Candida boidinii, Kluyveromyces lactis, Kluyveromyces marxianus, Aspergillus terreus, Aspergillus niger, Pichia pastoris, Rhizopus arrhizus, Rhizopus oryzae, Yarrowia lipolytica, Issatchenkia orientalis and the like. Exemplary eukaryotic methylotrophs include, for example, eukaryotic methylotrophs found in the genera Candida, Pichia, Ogataea, Kuraishia and Komagataella. Particularly useful methylotrophic host organisms include, for example, Hansenula polymoipha, Pichia pastoris, Candida boidinii and Pichia methanolica. It is understood that any suitable microbial host organism can be used to introduce metabolic and/or genetic modifications to produce a desired product.

Depending on the fatty alcohol, fatty aldehyde, fatty acid or isopropanol biosynthetic pathway constituents of a selected host microbial organism, the non-naturally occurring microbial organisms of the invention will include at least one exogenously expressed fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway-encoding nucleic acid and up to all encoding nucleic acids for one or more fatty alcohol, fatty aldehyde, fatty acid or isopropanol biosynthetic pathways. For example, fatty alcohol, fatty aldehyde, fatty acid or isopropanol biosynthesis can be established in a host deficient in a pathway enzyme or protein through exogenous expression of the corresponding encoding nucleic acid. In a host deficient in all enzymes or proteins of a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway, exogenous expression of all enzyme or proteins in the pathway can be included, although it is understood that all enzymes or proteins of a pathway can be expressed even if the host contains at least one of the pathway enzymes or proteins. For example, exogenous expression of all enzymes or proteins in a pathway for production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol can be included, such as a thiolase, a 3-oxoacyl-CoA reductase, a 3-hydroxyacyl-CoA dehydratase, an enoyl-CoA redutase, an acyl-CoA reductase (aldehyde forming) and an alcohol dehydrogenase, for production of a fatty alcohol.

Given the teachings and guidance provided herein, those skilled in the art will understand that the number of encoding nucleic acids to introduce in an expressible form will, at least, parallel the fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway deficiencies of the selected host microbial organism. Therefore, a non-naturally occurring microbial organism of the invention can have one, two, three, four, five, six, seven or eight up to all nucleic acids encoding the enzymes or proteins constituting a fatty alcohol, fatty aldehyde, fatty acid or isopropanol biosynthetic pathway disclosed herein. In some embodiments, the non-naturally occurring microbial organisms also can include other genetic modifications that facilitate or optimize fatty alcohol, fatty aldehyde, fatty acid or isopropanol biosynthesis or that confer other useful functions onto the host microbial organism One such other functionality can include, for example, augmentation of the synthesis of one or more of the fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway precursors such as acetyl-CoA, malonyl-ACP, malonyl-CoA or propionyl-CoA.

Generally, a host microbial organism is selected such that it produces the precursor of a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway, either as a naturally produced molecule or as an engineered product that either provides de novo production of a desired precursor or increased production of a precursor naturally produced by the host microbial organism. For example, acetyl-CoA is produced naturally in a host organism such as E. coli. A host organism can be engineered to increase production of a precursor, as disclosed herein. In addition, a microbial organism that has been engineered to produce a desired precursor can be used as a host organism and further engineered to express enzymes or proteins of a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway.

In some embodiments, a non-naturally occurring microbial organism of the invention is generated from a host that contains the enzymatic capability to synthesize fatty alcohol, fatty aldehyde, fatty acid or isopropanol. In this specific embodiment it can be useful to increase the synthesis or accumulation of a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway product to, for example, drive fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway reactions toward fatty alcohol, fatty aldehyde, fatty acid or isopropanol production. Increased synthesis or accumulation can be accomplished by, for example, overexpression of nucleic acids encoding one or more of the above-described fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway enzymes or proteins. Overexpression of the enzyme or enzymes and/or protein or proteins of the fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway can occur, for example, through exogenous expression of the endogenous gene or genes, or through exogenous expression of the heterologous gene or genes. Therefore, naturally occurring organisms can be readily generated to be non-naturally occurring microbial organisms of the invention, for example, producing fatty alcohol, fatty aldehyde, fatty acid or isopropanol, through overexpression of one, two, three, four, five, six, seven, or eight, that is, up to all nucleic acids encoding fatty alcohol, fatty aldehyde, fatty acid or isopropanol biosynthetic pathway enzymes or proteins. In addition, a non-naturally occurring organism can be generated by mutagenesis of an endogenous gene that results in an increase in activity of an enzyme in the fatty alcohol, fatty aldehyde, fatty acid or isopropanol biosynthetic pathway.

In particularly useful embodiments, exogenous expression of the encoding nucleic acids is employed. Exogenous expression confers the ability to custom tailor the expression and/or regulatory elements to the host and application to achieve a desired expression level that is controlled by the user. However, endogenous expression also can be utilized in other embodiments such as by removing a negative regulatory effector or induction of the gene's promoter when linked to an inducible promoter or other regulatory element. Thus, an endogenous gene having a naturally occurring inducible promoter can be up-regulated by providing the appropriate inducing agent, or the regulatory region of an endogenous gene can be engineered to incorporate an inducible regulatory element, thereby allowing the regulation of increased expression of an endogenous gene at a desired time. Similarly, an inducible promoter can be included as a regulatory element for an exogenous gene introduced into a non-naturally occurring microbial organism.

It is understood that, in methods of the invention, any of the one or more exogenous nucleic acids can be introduced into a microbial organism to produce a non-naturally occurring microbial organism of the invention. The nucleic acids can be introduced so as to confer, for example, a fatty alcohol, fatty aldehyde, fatty acid or isopropanol biosynthetic pathway onto the microbial organism. Alternatively, encoding nucleic acids can be introduced to produce an intermediate microbial organism having the biosynthetic capability to catalyze some of the required reactions to confer fatty alcohol, fatty aldehyde, fatty acid or isopropanol biosynthetic capability. For example, a non-naturally occurring microbial organism having a fatty alcohol, fatty aldehyde, fatty acid or isopropanol biosynthetic pathway can comprise at least two exogenous nucleic acids encoding desired enzymes or proteins, such as the combination of a thiolase and an acyl-CoA reductase (alcohol forming), or alternatively a 2-oxoacyl-CoA reductase and an acyl-CoA hydrolase, or alternatively a enoyl-CoA reductase and an acyl-CoA reductase (aldehyde forming), or alternatively a methanol methyltransferase and an acetone reductase, or alternatively a 3-hexulose-6-phosphate synthase and an enoyl ACP-reductase, and the like. Thus, it is understood that any combination of two or more enzymes or proteins of a biosynthetic pathway can be included in a non-naturally occurring microbial organism of the invention. Similarly, it is understood that any combination of three or more enzymes or proteins of a biosynthetic pathway can be included in a non-naturally occurring microbial organism of the invention, for example, a thiolase, an enoyl-CoA reductase and a aldehyde dehydrogenase (acid forming), or alternatively a 3-hydroxyacyl-coA dehydratase, an acyl-CoA:ACP acyltransferase and a thioesterase, or alternatively a 3-oxoacyl-CoA reductase, an acyl-CoA hydrolase and a carboxylic acid reductase, or alternatively a dihydroxyacetone synthase, a S-formylglutathione hydrolase and an acetoacetyl-CoA ligase, or alternatively a 6-phospho-3-hexuloisomerase, a β-hydroxyacyl-ACP reductase and a fatty alcohol forming acyl-CoA reductase, and so forth, as desired, so long as the combination of enzymes and/or proteins of the desired biosynthetic pathway results in production of the corresponding desired product. Similarly, any combination of four, five, six, seven, eight, nine, ten, eleven, twelve, thirteen, fourteen, fifteen, sixteen, seventeen, eighteen, nineteen, twenty or more enzymes or proteins of a biosynthetic pathway as disclosed herein can be included in a non-naturally occurring microbial organism of the invention, as desired, so long as the combination of enzymes and/or proteins of the desired biosynthetic pathway results in production of the corresponding desired product.

In addition to the biosynthesis of fatty alcohol, fatty aldehyde, fatty acid or isopropanol as described herein, the non-naturally occurring microbial organisms and methods of the invention also can be utilized in various combinations with each other and/or with other microbial organisms and methods well known in the art to achieve product biosynthesis by other routes. For example, one alternative to produce fatty alcohol, fatty aldehyde, fatty acid or isopropanol other than use of the fatty alcohol, fatty aldehyde, fatty acid or isopropanol producers is through addition of another microbial organism capable of converting a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway intermediate to fatty alcohol, fatty aldehyde, fatty acid or isopropanol. One such procedure includes, for example, the fermentation of a microbial organism that produces a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway intermediate. The fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway intermediate can then be used as a substrate for a second microbial organism that converts the fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway intermediate to fatty alcohol, fatty aldehyde, fatty acid or isopropanol. The fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway intermediate can be added directly to another culture of the second organism or the original culture of the fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway intermediate producers can be depleted of these microbial organisms by, for example, cell separation, and then subsequent addition of the second organism to the fermentation broth can be utilized to produce the final product without intermediate purification steps.

In other embodiments, the non-naturally occurring microbial organisms and methods of the invention can be assembled in a wide variety of subpathways to achieve biosynthesis of for example, fatty alcohol, fatty aldehyde, fatty acid or isopropanol. In these embodiments, biosynthetic pathways for a desired product of the invention can be segregated into different microbial organisms, and the different microbial organisms can be co-cultured to produce the final product. In such a biosynthetic scheme, the product of one microbial organism is the substrate for a second microbial organism until the final product is synthesized. For example, the biosynthesis of fatty alcohol, fatty aldehyde, fatty acid or isopropanol can be accomplished by constructing a microbial organism that contains biosynthetic pathways for conversion of one pathway intermediate to another pathway intermediate or the product. Alternatively, fatty alcohol, fatty aldehyde, fatty acid or isopropanol also can be biosynthetically produced from microbial organisms through co-culture or co-fermentation using two organisms in the same vessel, where the first microbial organism produces a fatty alcohol, fatty aldehyde, fatty acid or isopropanol intermediate and the second microbial organism converts the intermediate to fatty alcohol, fatty aldehyde, fatty acid or isopropanol.

Given the teachings and guidance provided herein, those skilled in the art will understand that a wide variety of combinations and permutations exist for the non-naturally occurring microbial organisms and methods of the invention together with other microbial organisms, with the co-culture of other non-naturally occurring microbial organisms having subpathways and with combinations of other chemical and/or biochemical procedures well known in the art to produce fatty alcohol, fatty aldehyde, fatty acid or isopropanol.

Similarly, it is understood by those skilled in the art that a host organism can be selected based on desired characteristics for introduction of one or more gene disruptions to increase production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol. Thus, it is understood that, if a genetic modification is to be introduced into a host organism to disrupt a gene, any homologs, orthologs or paralogs that catalyze similar, yet non-identical metabolic reactions can similarly be disrupted to ensure that a desired metabolic reaction is sufficiently disrupted. Because certain differences exist among metabolic networks between different organisms, those skilled in the art will understand that the actual genes disrupted in a given organism may differ between organisms. However, given the teachings and guidance provided herein, those skilled in the art also will understand that the methods of the invention can be applied to any suitable host microorganism to identify the cognate metabolic alterations needed to construct an organism in a species of interest that will increase fatty alcohol, fatty aldehyde, fatty acid or isopropanol biosynthesis. In a particular embodiment, the increased production couples biosynthesis of fatty alcohol, fatty aldehyde, fatty acid or isopropanol to growth of the organism, and can obligatorily couple production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol to growth of the organism if desired and as disclosed herein.

Sources of encoding nucleic acids for a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway enzyme or protein can include, for example, any species where the encoded gene product is capable of catalyzing the referenced reaction. Such species include both prokaryotic and eukaryotic organisms including, but not limited to, bacteria, including archaea and eubacteria, and eukaryotes, including yeast, plant, insect, animal, and mammal, including human. Exemplary species for such sources include, for example, Escherichia coli, 255956237 Penicillium chrysogenum Wisconsin 54-1255, Acetobacter pasteurians, Acidaminococcus fermentans, Acinetobacter baumannii Naval-82, Acinetobacter baylyi, Acinetobacter calcoaceticus, Acinetobacter sp. ADP1, Acinetobacter sp. Strain M-1, Actinobacillus succinogenes, Actinobacillus succinogenes 130Z, Aedes aegypti, Allochromatium vinosum DSM 180, Aminomonas aminovorus, Anabaena variabilis ATCC 29413, Anaerobiospirillum succiniciproducens, Aquifex aeolicus, Arabidopsis thaliana, Archaeoglobus fulgidus, Archaeoglobus fulgidus DSM 4304, Arthrobacter globiformis, Ascaris suum, Aspergillus fumigatus, Aspergillus nidulans, Aspergillus niger, Aspergillus niger CBS 513.88, Aspergillus terreus NIH2624, Aspergillus Synechococcus elongatus PCC 6301, Azotobacter vinelandii DJ, B. subtilis 168, Bacillus alcalophilus ATCC 27647, Bacillus anthracia, Bacillus azotoformans LMG 9581, Bacillus cereus, Bacillus cereus ATCC 14579, Bacillus coagulans 36D1, Bacillus megaterium, Bacillus methanolicus MGA3, Bacillus methanolicus PB1, Bacillus selenitireducens MLS10, Bacillus sp. SG-1, Bacillus sphaericus, Bacillus subtilis, Bacteroides fragilis, Bifidobacterium bifidum, Bifidobacterium longum NCC2705, Bombyx mori, Bos taurus, Bradyrhizobium japonicum, Bradyrhizobium japonicum USDA110, Brassica juncea, Brassica napsus, Burkholderia ambifaria AMMD, Burkholderia cenocepacia, Burkholderia cepacia, Burkholderia multivorans, Burkholderia phymatum, Burkholderia pyrrocinia, Burkholderia stabilis, Burkholderia thailandensis E264, Burkholderiales bacterium Joshi_001, butyrate producing bacterium L2-50, Caenorhabditis elegans, Campylobacter curvus 525.92, Campylobacter jejuni, Candida albicans, Candida boidinii, Candida methylica, Candida parapsilosis, Candida tropicalis, Candida tropicalis MYA-3404, Candida tropicalis MYA-3404, Candida tropicalis, Carboxydothermus hydrogenoformans, Carboxydothermus hydrogenoformans Z-2901, Carthamus tinctorius, Caulobacter sp. AP07, Chlamydomonas reinhardtii, Chlorobium limicola, Chlorobium phaeobacteroides DSM 266, Chlorobium tepidum, Chloroflexus aurantiacus, Cinnamonum camphorum, Citrobacter koseri ATCC BAA-895, Citrus junos, Clostridium acetobutylicum, Clostridium acetobutylicum ATCC 824, Clostridium aminobutyricum, Clostridium beijerinckii, Clostridium beijerinckii NCIMB 8052, Clostridium carboxidivorans P7, Clostridium cellulolyticum H10, Clostridium cellulovorans 743B, Clostridium kluyveri, Clostridium kluyveri DSM 555, Clostridium ljungdahli, Clostridium ljungdahlii DSM 13528, Clostridium pasteurianum, Clostridium pasteurianum DSM 525, Clostridium perfringens, Clostridium perfringens ATCC 13124, Clostridium perfringens str. 13, Clostridium phytofermentans ISDg, Clostridium saccharoperbutylacetonicum, Clostridium symbiosum, Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 14067, Corynebacterium glutamicum R, Corynebacterium sp., Corynebacterium sp. U-96, Corynebacterium ulcerans, Corynebacterium variabile, Cryptosporidium parvum Iowa II, Cuphea hookeriana, Cuphea palustris, Cupriavidus necator, Cupriavidus necator N-1, Cupriavidus taiwanensis, Cyanobium PCC7001, Cyanothece sp. PCC 7425, Danio rerio, Desulfatibacillum alkenivorans AK-01, Desulfitobacterium hafniense, Desulfitobacterium metallireducens DSM 15288, Desulfococcus oleovorans Hxd3, Desulfotomaculum reducens MI-1, Desulfovibrio africanus, Desulfovibrio africanus str. Walvis Bay, Desulfovibrio alaskensis, Desulfovibrio desulfuricans subsp. desulfuricans str. ATCC 27774, Desulfovibrio fructosovorans JJ, Desulfovibrio vulgaris str. ‘Miyazaki F’, Desulfovibrio vulgaris str. Hildenborough, Dictyostelium discoideum AX4, E. coli, Erythrobacter sp. NAP1, Escherichia coli DH1, Escherichia coli K-12, Escherichia coli K-12 MG1655, Escherichia coli K-12 MG1655 niger CBS 513.88, Escherichia coli LW1655F+, Escherichia coli MG1655, Escherichia coli str. K-12 substr. MG1655, Euglena gracilis, Flavobacterium frigoris, Fusobacterium nucleatum, Geobacillus sp. GHH01, Geobacillus sp. M10EXG, Geobacillus sp. Y4.1MC1, Geobacillus themodenitrificans NG80-2, Geobacillus thermodenitnficans, Geobacter bemidjiensis Bem, Geobacter metallireducens GS-15, Geobacter sulfurreducens, Geobacter sulfurreducens PCA, Haemophilus influenza, Haloarcula marismortui, Haloarcula marismortui ATCC 43049, Halomonas sp. HTNK1, Helianthus annuus, Helicobacter pylori, Helicobacter pylori 26695, Homo sapiens, human gut metagenome, Hydrogenobacter thermophilus, Hyphomicrobium denitrificans ATCC 51888, Hyphomicrobium zavarzinii, Kineococcus radiotolerans, Klebsiella pneumonia, Klebsiella pneumoniae, Klebsiella pneumoniae subsp. pneumoniae MGH 78578, Kluyveromyces lactis, Kluyveromyces lactis NRRL Y-1140, Lactobacillus acidophilus, Lactobacillus brevis ATCC 367, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus reuteri, Lactococcus lactis, Lactococcus lactis subsp. lactis, Leifsonia sp. S749, Leuconostoc mesenteroides, Listeria monocytogenes, Lyngbya sp. PCC 8106, Lysinibacillus fusiformis, Lysinibacillus sphaericus, Mannheimia succiniciproducens, marine gamma proteobacterium HTCC2080, Marinobacter aquaeolei, Megathyrsus maximus, Mesorhizobium loti, Mesorhizobium loti M4FF303099, Metallosphaera sedula, Metallosphaera sedula, Metarhizium acridum CQMa 102, Methanosarcina acetivorans, Methanosarcina acetivorans C2A, Methanosarcina barkeri, Methanosarcina mazei Tuc01, Methanosarcina thermophila, Methanothermobacter thermautotrophicus, Methylobacillus flagellates, Methylobacillus flagellates KT, Methylobacter marinus, Methylobacterium extorquens, Methylobacterium extorquens AM1, Methylococcus capsulatis, Methylomicrobium album BG8, Methylomonas aminofaciens, Methylovorus glucosetrophus SIP3-4, Methylovorus sp. MP688, Moorella thermoacetica, Moorella thermoacetica ATCC 39073, Mus musculus, Mycobacter sp. strain JC1 DSM 3803, Mycobacterium avium subsp. paratuberculosis K-10, Mycobacterium bovis BCG, Mycobacterium gastri, Mycobacterium kansasii ATCC 12478, Mycobacterium marinum M, Mycobacterium smegmatis, Mycobacterium smegmatis MC2 155, Mycobacterium smegmatis str. MC2 155, Mycobacterium sp. strain JLS, Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Neurospora crassa OR74A, Nicotiana tabacum, Nitrosopumilus salaria BD31, Nitrososphaera gargensis Ga9.2, Nocardia brasiliensis, Nocardia farcinica IFM 10152, Nocardia iowensis, Nocardia iowensis (sp. NRRL 5646), Nodularia spumigena CCY9414, Nostoc azollae, Nostoc sp. PCC 7120, Ogataea parapolymorpha DL-1 (Hansenula polymorpha DL-1), Oxalobacter formigenes, Paenibacillus peoriae KCTC 3763, Paracoccus denitrificans, Pelobacter carbinolicus DSM 2380, Penicillium chrysogenum, Perkinsus marinus ATCC 50983, Photobacterium leiognathi PL741, Photobacterium phosphoreum, Photobacterium profundum 3TCK Phtomonas sp., Pichia pastoris, Pichia pastoris GS115, Picrophilus torridus DSM9790, Plasmodium falciparum, Porphyromonas gingivalis, Porphyromonas gingivalis W83, Prochlorococcus marinus MIT 9312, Propionibacterium acnes, Propionibacterium fredenreichii sp. shermanii, Propionibacterium freudenreichii, Propionibacterium freundenreichii subsp. Shermanii, Propionigenium modestum, Pseudomonas aeruginosa, Pseudomonas aeruginosa PA01, Pseudomonas fluorescens, Pseudomonas fluorescens Pf0-1, Pseudomonas knackmussii, Pseudomonas knackmussii (B13), Pseudomonas mendocina, Pseudomonas putida, Pseudomonas putida GB-1, Pseudomonas putida GB-1 Trypanosoma brucei, Pseudomonas sp, Pseudomonas sp. CF600, Pseudomonas stutzeri, Pseudomonas syringae, Pseudomonas syringae pv. syringae B728a, Pyrobaculum aerophilum str. IM2, Pyrococcus abyssi, Pyrococcus furiosus, Pyrococcus horikoshii OT3, Ralstonia eutropha, Ralstonia eutropha H16, Ralstonia metallidurans, Rattus norvegicus, Rhizobium leguminosarum, Rhizopus oryzae, Rhodobacter capsulatus, Rhodobacter sphaeroides, Rhodobacter sphaeroides ATCC 17025, Rhodococcus erythropolis SK121, Rhodococcus opacus B4, Rhodopseudomonas palustris, Rhodopseudomonas palustris CGA009, Rhodopseudomonas palustris DX-1, Rhodospirillum rubrum, Roseiflexus castenholzii, Saccahromyces cerevisiae, Saccharomyces cerevisiae S288c, Salmonella enteric, Salmonella enterica, Salmonella enterica LT2, Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, Salmonella enterica Typhimurium, Salmonella typhimurium, Salmonella typhimurium LT2, Schizosaccharomyces pombe, Sebaldella termitidis ATCC 33386, Shewanella oneidensis MR-1, Shigella dysenteriae, Shigella flexneri, Shigella sonnei, Simmondsia chinensis, Sinorhizobium meliloti 1021, Solanum lycopersicum, Sordaria macrospora, Staphylococcus aureus, Staphylococcus aureus MW2, Streptococcus mutans, Streptococcus mutans UA159, Streptococcus pneumoniae, Streptococcus sanguinis, Streptomyces anulatus, Streptomyces avermitilis MA-4680, Streptomyces avermitillis, Streptomyces cinnamonensis, Streptomyces coelicolor, Streptomyces glaucescens, Streptomyces griseus subsp. griseus NBRC 13350, Streptomyces luridus, Streptomyces sp CL190, Streptomyces sp CL190, Streptomyces sp. KO-3988, Streptomyces viridochromogenes, Streptomyces wedmorensis, Sulfolobus acidocaldarius, Sulfolobus solfataricus, Sulfolobus solfataricus P-2, Sulfolobus tokodaii, Sulfurihydrogenibium subterraneum, Sulfurimonas denitnficans, Sus scrofa, Synechococcus elongatus PCC 6301, Synechococcus elongatus PCC7942, Synechococcus sp. PCC 7002, Synechocystis str. PCC 6803, Syntrophobacter fumaroxidans, Syntrophus aciditrophicus, Thauera aromatic, Thermoanaerobacter ethanolicus JW 200, Thermoanaerobacter pseudethanolicus ATCC 33223, Thermoanaerobacter sp. X514, Thermoanaerobacter tengcongensis MB4, Thermoanaerobobacter brockii, Thermococcus kodakaraensis, Thermococcus litoralis, Thermomyces lanuginosus, Thermoplasma acidophilum, Thermoproteus neutrophilus, Thermotoga maritime, Thiocapsa roseopersicina, Treponema denticola, Trichomonas vaginalis G3, Triticum aestivum, Trypanosoma brucei, Trypanosoma cruzi strain CL Brener, Tsukamurella paurometabola DSM 20162, Umbellularia californica, uncultured organism, Veillonella parvula, Vibrio harveyi ATCC BAA-1116, Xanthobacter autotrophicus Py2, Xenopus tropicalis, Yarrowia lipolytica, Yersinia frederiksenii, Zea mays, Zoogloea ramigera, Zymomonas mobilis, Zymomonas mobilis subsp. mobilis ZM4, Clostridium beijerinickii, Deinococcus radiodurans R1, Aquifex aeolicus VF5, Methanocaldococcus janaschii, Yersinia pestis, Bifidobacterium animalis lactis, Bifidobacterium dentium ATCC 27678, Bifidobacterium pseudolongum subsp. globosum, Bifidobacterium breve, Lactobacillus paraplantarum, Corynebacterium glutamicum ATCC 13032, as well as other exemplary species disclosed herein or available as source organisms for corresponding genes. However, with the complete genome sequence available for now more than 550 species (with more than half of these available on public databases such as the NCBI), including 395 microorganism genomes and a variety of yeast, fungi, plant, and mammalian genomes, the identification of genes encoding the requisite fatty alcohol, fatty aldehyde, fatty acid or isopropanol biosynthetic activity for one or more genes in related or distant species, including for example, homologues, orthologs, paralogs and nonorthologous gene displacements of known genes, and the interchange of genetic alterations between organisms is routine and well known in the art. Accordingly, the metabolic alterations allowing biosynthesis of fatty alcohol, fatty aldehyde, fatty acid or isopropanol described herein with reference to a particular organism such as E. coli can be readily applied to other microorganisms, including prokaryotic and eukaryotic organisms alike. Given the teachings and guidance provided herein, those skilled in the art will know that a metabolic alteration exemplified in one organism can be applied equally to other organisms.

In some instances, such as when an alternative fatty alcohol, fatty aldehyde, fatty acid or isopropanol biosynthetic pathway exists in an unrelated species, fatty alcohol, fatty aldehyde, fatty acid or isopropanol biosynthesis can be conferred onto the host species by, for example, exogenous expression of a paralog or paralogs from the unrelated species that catalyzes a similar, yet non-identical metabolic reaction to replace the referenced reaction. Because certain differences among metabolic networks exist between different organisms, those skilled in the art will understand that the actual gene usage between different organisms may differ. However, given the teachings and guidance provided herein, those skilled in the art also will understand that the teachings and methods of the invention can be applied to all microbial organisms using the cognate metabolic alterations to those exemplified herein to construct a microbial organism in a species of interest that will synthesize fatty alcohol, fatty aldehyde, fatty acid or isopropanol. A nucleic acid molecule encoding a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway enzyme or protein of the invention can also include a nucleic acid molecule that hybridizes to a nucleic acid disclosed herein by SEQ ID NO, GenBank and/or GI number or a nucleic acid molecule that hybridizes to a nucleic acid molecule that encodes an amino acid sequence disclosed herein by SEQ ID NO, GenBank and/or GI number. Hybridization conditions can include highly stringent, moderately stringent, or low stringency hybridization conditions that are well known to one of skill in the art such as those described herein. Similarly, a nucleic acid molecule that can be used in the invention can be described as having a certain percent sequence identity to a nucleic acid disclosed herein by SEQ ID NO, GenBank and/or GI number or a nucleic acid molecule that hybridizes to a nucleic acid molecule that encodes an amino acid sequence disclosed herein by SEQ ID NO, GenBank and/or GI number. For example, the nucleic acid molecule can have at least 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to a nucleic acid described herein.

Stringent hybridization refers to conditions under which hybridized polynucleotides are stable. As known to those of skill in the art, the stability of hybridized polynucleotides is reflected in the melting temperature (T_(m)) of the hybrids. In general, the stability of hybridized polynucleotides is a function of the salt concentration, for example, the sodium ion concentration and temperature. A hybridization reaction can be performed under conditions of lower stringency, followed by washes of varying, but higher, stringency. Reference to hybridization stringency relates to such washing conditions. Highly stringent hybridization includes conditions that permit hybridization of only those nucleic acid sequences that form stable hybridized polynucleotides in 0.018M NaCl at 65° C., for example, if a hybrid is not stable in 0.018M NaCl at 65° C., it will not be stable under high stringency conditions, as contemplated herein. High stringency conditions can be provided, for example, by hybridization in 50% formamide, 5×Denhart's solution, 5×SSPE, 0.2% SDS at 42° C., followed by washing in 0.1×SSPE, and 0.1% SDS at 65° C. Hybridization conditions other than highly stringent hybridization conditions can also be used to describe the nucleic acid sequences disclosed herein. For example, the phrase moderately stringent hybridization refers to conditions equivalent to hybridization in 50% formamide, 5×Denhart's solution, 5×SSPE, 0.2% SDS at 42° C., followed by washing in 0.2×SSPE, 0.2% SDS, at 42° C. The phrase low stringency hybridization refers to conditions equivalent to hybridization in 10% formamide, 5×Denhart's solution, 6×SSPE, 0.2% SDS at 22° C., followed by washing in 1×SSPE, 0.2% SDS, at 37° C. Denhart's solution contains 1% Ficoll, 1% polyvinylpyrolidone, and 1% bovine serum albumin (BSA). 20×SSPE (sodium chloride, sodium phosphate, ethylene diamide tetraacetic acid (EDTA)) contains 3M sodium chloride, 0.2M sodium phosphate, and 0.025 M (EDTA). Other suitable low, moderate and high stringency hybridization buffers and conditions are well known to those of skill in the art and are described, for example, in Sambrook et al., Molecular Cloning: A Laboratory Manual, Third Ed., Cold Spring Harbor Laboratory, New York (2001); and Ausubel et al., Current Protocols in Molecular Biology, John Wiley and Sons, Baltimore, Md. (1999).

A nucleic acid molecule encoding a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway enzyme or protein of the invention can have at least a certain sequence identity to a nucleotide sequence disclosed herein. According, in some aspects of the invention, a nucleic acid molecule encoding a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway enzyme or protein has a nucleotide sequence of at least 65% identity, at least 70% identity, at least 75% identity, at least 80% identity, at least 85% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to a nucleic acid disclosed herein by SEQ ID NO, GenBank and/or GI number or a nucleic acid molecule that hybridizes to a nucleic acid molecule that encodes an amino acid sequence disclosed herein by SEQ ID NO, GenBank and/or GI number.

Sequence identity (also known as homology or similarity) refers to sequence similarity between two nucleic acid molecules or between two polypeptides. Identity can be determined by comparing a position in each sequence, which may be aligned for purposes of comparison. When a position in the compared sequence is occupied by the same base or amino acid, then the molecules are identical at that position. A degree of identity between sequences is a function of the number of matching or homologous positions shared by the sequences. The alignment of two sequences to determine their percent sequence identity can be done using software programs known in the art, such as, for example, those described in Ausubel et al., Current Protocols in Molecular Biology, John Wiley and Sons, Baltimore, Md. (1999). Preferably, default parameters are used for the alignment. One alignment program well known in the art that can be used is BLAST set to default parameters. In particular, programs are BLASTN and BLASTP, using the following default parameters: Genetic code=standard; filter=none; strand=both; cutoff=60; expect=10; Matrix=BLOSUM62; Descriptions=50 sequences; sort by=HIGH SCORE; Databases=non-redundant, GenBank+EMBL+DDBJ+PDB+GenBank CDS translations+SwissProtein+SPupdate+PIR. Details of these programs can be found at the National Center for Biotechnology Information.

Methods for constructing and testing the expression levels of a non-naturally occurring fatty alcohol, fatty aldehyde, fatty acid or isopropanol-producing host can be performed, for example, by recombinant and detection methods well known in the art Such methods can be found described in, for example, Sambrook et al., Molecular Cloning: A Laboratory Manual, Third Ed., Cold Spring Harbor Laboratory, New York (2001); and Ausubel et al., Current Protocols in Molecular Biology, John Wiley and Sons, Baltimore, Md. (1999).

Exogenous nucleic acid sequences involved in a pathway for production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol can be introduced stably or transiently into a host cell using techniques well known in the art including, but not limited to, conjugation, electroporation, chemical transformation, transduction, transfection, and ultrasound transformation. For exogenous expression in E. coli or other prokaryotic cells, some nucleic acid sequences in the genes or cDNAs of eukaryotic nucleic acids can encode targeting signals such as an N-terminal mitochondrial or other targeting signal, which can be removed before transformation into prokaryotic host cells, if desired. For example, removal of a mitochondrial leader sequence led to increased expression in E. coli (Hoffineister et al., J. Biol. Chem. 280:4329-4338 (2005)). For exogenous expression in yeast or other eukaryotic cells, genes can be expressed in the cytosol without the addition of leader sequence, or can be targeted to mitochondrion or other organelles, or targeted for secretion, by the addition of a suitable targeting sequence such as a mitochondrial targeting or secretion signal suitable for the host cells. Thus, it is understood that appropriate modifications to a nucleic acid sequence to remove or include a targeting sequence can be incorporated into an exogenous nucleic acid sequence to impart desirable properties. Furthermore, genes can be subjected to codon optimization with techniques well known in the art to achieve optimized expression of the proteins.

An expression vector or vectors can be constructed to include one or more fatty alcohol, fatty aldehyde, fatty acid or isopropanol biosynthetic pathway encoding nucleic acids as exemplified herein operably linked to expression control sequences functional in the host organism. Expression vectors applicable for use in the microbial host organisms of the invention include, for example, plasmids, phage vectors, viral vectors, episomes and artificial chromosomes, including vectors and selection sequences or markers operable for stable integration into a host chromosome. Additionally, the expression vectors can include one or more selectable marker genes and appropriate expression control sequences. Selectable marker genes also can be included that, for example, provide resistance to antibiotics or toxins, complement auxotrophic deficiencies, or supply critical nutrients not in the culture media. Expression control sequences can include constitutive and inducible promoters, transcription enhancers, transcription terminators, and the like which are well known in the art. When two or more exogenous encoding nucleic acids are to be co-expressed, both nucleic acids can be inserted, for example, into a single expression vector or in separate expression vectors. For single vector expression, the encoding nucleic acids can be operationally linked to one common expression control sequence or linked to different expression control sequences, such as one inducible promoter and one constitutive promoter. The transformation of exogenous nucleic acid sequences involved in a metabolic or synthetic pathway can be confirmed using methods well known in the art. Such methods include, for example, nucleic acid analysis such as Northern blots or polymerase chain reaction (PCR) amplification of mRNA, or immunoblotting for expression of gene products, or other suitable analytical methods to test the expression of an introduced nucleic acid sequence or its corresponding gene product. It is understood by those skilled in the art that the exogenous nucleic acid is expressed in a sufficient amount to produce the desired product, and it is further understood that expression levels can be optimized to obtain sufficient expression using methods well known in the art and as disclosed herein.

In some embodiments, the invention provides a method for producing a compound of Formula (I):

wherein R₁ is C₁₋₂₄ linear alkyl; R₂ is CH₂OH, CHO, or COOH; R₃ is H, OH, or oxo (═O); and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four, comprising culturing a non-naturally occurring microbial organism of the invention under conditions and for a sufficient period of time to produce the compound of Formula (I).

In some aspects of the invention, the microbial organism used in a method of the invention includes a non-naturally occurring having: (i) a formaldehyde fixation pathway; (ii) a formate assimilation pathway; and/or (iii) a methanol metabolic pathway as depicted in FIGS. 1 and 10, and a MI-FAE cycle or a MD-FAE cycle in combination with a termination pathway as depicted in FIGS. 2, 7 and 8, wherein said formaldehyde fixation pathway comprises: (1) 1B and 1C; (2) 1D; or (3) 1D and 1Z, wherein 1B is a 3-hexulose-6-phosphate synthase, wherein 1C is a 6-phospho-3-hexuloisomerase, wherein 1D is a dihydroxyacetone synthase, wherein 1Z is a fructose-6-phosphate aldolase, wherein said formate assimilation pathway comprises a pathway selected from: (4) 1E; (5) 1F, and 1 G; (6) 1H, 1I, 1J, and 1K; (7) 1H, 1I, 1J, 1L, 1M, and 1N; (8) 1E, 1H, 1I, 1J, 1L, 1M, and 1N; (9) 1F, 1G, 1H, 1I, 1J, 1L, 1M, and 1N; (10) 1K, 1H, 1I, 1J, 1L, 1M, and 1N; and (11) 1H, 1I, 1J, 1O, and 1P, wherein 1E is a formate reductase, 1F is a formate ligase, a formate transferase, or a formate synthetase, wherein 1G is a formyl-CoA reductase, wherein 1H is a formyltetrahydrofolate synthetase, wherein 1I is a methenyltetrahydrofolate cyclohydrolase, wherein 1J is a methylenetetrahydrofolate dehydrogenase, wherein 1K is a formaldehyde-forming enzyme or spontaneous, wherein 1L is a glycine cleavage system, wherein 1M is a serine hydroxymethyltransferase, wherein 1N is a serine deaminase, wherein 10 is a methylenetetrahydrofolate reductase, wherein 1P is an acetyl-CoA synthase, wherein said methanol metabolic pathway comprises a pathway selected from: (12) 10J; (13) 10A, (14) 10A and 10B; (15) 10A, 10B and 10C; (16) 10J, 10K and 10C; (17) 10J, 10M, and 10N; (18) 10J and 10L; (19) 10J, 10L and 10G; (20) 10J, 10L, and 10I; (21) 10A, 10B, 10C, 10D, and 10E; (22) 10A, 10B, 10C, 10D, and 10F; (23) 10J, 10K, 10C, 10D, and 10E; (24) 10J, 10K, 10C, 10D, and 10F; (25) 10J, 10M, 10N, and 10O; (26) 10A, 10B, 10C, 10D, 10E, and 10G; (27) 10A, 10B, 10C, 10D, 10F, and 10G; (28) 10J, 10K, 10C, 10D, 10E, and 10G; (29) 10J, 10K, 10C, 10D, 10F, and 10G; (30) 10J, 10M, 10N, 10O, and 10G; (31) 10A, 10B, 10C, 10D, 10E, and 10I; (32) 10A, 10B, 10C, 10D, 10F, and 10I; (33) 10J, 10K, 10C, 10D, 10E, and 10I; (34) 10J, 10K, 10C, 10D, 10F, and 10I; and (35) 10J, 10M, 10N, 10O, and 10I, wherein 10A is a methanol methyltransferase, wherein 10B is a methylenetetrahydrofolate reductase, wherein 10C is a methylenetetrahydrofolate dehydrogenase, wherein 10D is a methenyltetrahydrofolate cyclohydrolase, wherein 10E is a formyltetrahydrofolate deformylase, wherein 10F is a formyltetrahydrofolate synthetase, wherein 10G is a formate hydrogen lyase, wherein 10I is a formate dehydrogenase, wherein 10J is a methanol dehydrogenase, wherein 10K is a formaldehyde activating enzyme or spontaneous, wherein 10L is a formaldehyde dehydrogenase, wherein 10M is a S-(hydroxymethyl)glutathione synthase or spontaneous, wherein 10N is a glutathione-dependent formaldehyde dehydrogenase, wherein 10O is a S-formylglutathione hydrolase, wherein the MI-FAE cycle includes one or more thiolase, one or more 3-oxoacyl-CoA reductase, one or more 3-hydroxyacyl-CoA dehydratase, and one or more enoyl-CoA reductase, wherein the MD-FAE cycle includes one or more elongase, one or more 3-oxoacyl-CoA reductase, one or more 3-hydroxyacyl-CoA dehydratase, and one or more enoyl-CoA reductase, wherein the termination pathway includes a pathway selected from: (36) 2H; (37) 2K and 2L; (38) 2E and 2N; (39) 2K, 2J, and 2N; (40) 2E; (41) 2K and 2J; (42) 2H and 2N; (43) 2K, 2L, and 2N; (44) 2E and 2F; (45) 2K, 2J, and 2F; (46) 2H, 2N, and 2F; (47) 2K, 2L, 2N, and 2F; (48) 2G; and (49) 2P, wherein 2E is an acyl-CoA reductase (aldehyde forming), wherein 2F is an alcohol dehydrogenase, wherein 2G is an acyl-CoA reductase (alcohol forming), wherein 2H is an acyl-CoA hydrolase, acyl-CoA transferase or acyl-CoA synthase, wherein 2J is an acyl-ACP reductase, wherein 2K is an acyl-CoA:ACP acyltransferase, wherein 2L is a thioesterase, wherein 2N is an aldehyde dehydrogenase (acid forming) or a carboxylic acid reductase, wherein 2P is an acyl-ACP reductase (alcohol forming) wherein an enzyme of the formaldehyde fixation pathway, the formate assimilation pathway, the methanol metabolic pathway, the MI-FAE cycle, MD-FAE cycle or termination pathway is encoded by at least one exogenous nucleic acid and is expressed in a sufficient amount to produce a compound of Formula (I):

wherein R₁ is C₁₋₂₄ linear alkyl; R₂ is CH₂OH, CHO, or COOH; R₃ is H, OH, or oxo (═O); and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four, wherein the substrate of each of said enzymes of the MI-FAE cycle, the MD-FAE cycle and the termination pathway are independently selected from a compound of Formula (II), malonyl-CoA, propionyl-CoA or acetyl-CoA:

wherein R₁ is C₁₋₂₄ linear alkyl; R₃ is H, OH, or oxo (═O); R₄ is S-CoA, ACP, OH or H; and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four; wherein said one or more enzymes of the MI-FAE cycle are each selective for a compound of Formula (II) having a number of carbon atoms at R₁ that is no greater than the number of carbon atoms at R₁ of said compound of Formula (I), wherein said one or more enzymes of the MD-FAE cycle are each selective for a compound of Formula (II) having a number of carbon atoms at R₁ that is no greater than the number of carbon atoms at R₁ of said compound of Formula (I), and wherein said one or more enzymes of the termination pathway are each selective for a compound of Formula (II) having a number of carbon atoms at R₁ that is no less than the number of carbon atoms at R₁ of said compound of Formula (I).

In some aspects of the invention, the microbial organism used in a method of the invention includes a non-naturally occurring having: (i) a formaldehyde fixation pathway; (ii) a formate assimilation pathway; and/or (iii) a methanol metabolic pathway as depicted in FIGS. 1 and 10, and a FAACPE cycle in combination with a termination pathway as depicted in FIG. 12, wherein said formaldehyde fixation pathway comprises: (1) 1B and 1C; (2) 1D; (3) 1D and 1Z, wherein 1B is a 3-hexulose-6-phosphate synthase, wherein 1C is a 6-phospho-3-hexuloisomerase, wherein 1D is a dihydroxyacetone synthase, wherein 1Z is a fructose-6-phosphate aldolase, wherein said formate assimilation pathway comprises a pathway selected from: (4) 1E; (5) 1F, and 1G; (6) 1H, 1I, 1J, and 1K; (7) 1H, 1I, 1J, 1L, 1M, and 1N; (8) 1E, 1H, 1I, 1J, 1L, 1M, and 1N; (9) 1F, 1G, 1H, 1I, 1J, 1L, 1M, and 1N; (10) 1K, 1H, 1I, 1J, 1L, 1M, and 1N; and (11) 1H, 1I, 1J, 1O, and 1P, wherein 1E is a formate reductase, 1F is a formate ligase, a formate transferase, or a formate synthetase, wherein 1G is a formyl-CoA reductase, wherein 1H is a formyltetrahydrofolate synthetase, wherein 1I is a methenyltetrahydrofolate cyclohydrolase, wherein 1J is a methylenetetrahydrofolate dehydrogenase, wherein 1K is a formaldehyde-forming enzyme or spontaneous, wherein 1L is a glycine cleavage system, wherein 1M is a serine hydroxymethyltransferase, wherein 1N is a serine deaminase, wherein 10 is a methylenetetrahydrofolate reductase, wherein 1P is an acetyl-CoA synthase, wherein said methanol metabolic pathway comprises a pathway selected from: (12) 10J; (13) 10A, (14) 10A and 10B; (15) 10A, 10B and 10C; (16) 10J, 10K and 10C; (17) 10J, 10M, and 10N; (18) 10J and 10L; (19) 10J, 10L and 10G; (20) 10J, 10L, and 10I; (21) 10A, 10B, 10C, 10D, and 10E; (22) 10A, 10B, 10C, 10D, and 10F; (23) 10J, 10K, 10C, 10D, and 10E; (24) 10J, 10K, 10C, 10D, and 10F; (25) 10J, 10M, 10N, and 10O; (26) 10A, 10B, 10C, 10D, 10E, and 10G; (27) 10A, 10B, 10C, 10D, 10F, and 10G; (28) 10J, 10K, 10C, 10D, 10E, and 10G; (29) 10J, 10K, 10C, 10D, 10F, and 10G; (30) 10J, 10M, 10N, 10O, and 10G; (31) 10A, 10B, 10C, 10D, 10E, and 10I; (32) 10A, 10B, 10C, 10D, 10F, and 10I; (33) 10J, 10K, 10C, 10D, 10E, and 10I; (34) 10J, 10K, 10C, 10D, 10F, and 10I; and (35) 10J, 10M, 10N, 10O, and 10I, wherein 10A is a methanol methyltransferase, wherein 10B is a methylenetetrahydrofolate reductase, wherein 10C is a methylenetetrahydrofolate dehydrogenase, wherein 10D is a methenyltetrahydrofolate cyclohydrolase, wherein 10E is a formyltetrahydrofolate deformylase, wherein 10F is a formyltetrahydrofolate synthetase, wherein 10G is a formate hydrogen lyase, wherein 10I is a formate dehydrogenase, wherein 10J is a methanol dehydrogenase, wherein 10K is a formaldehyde activating enzyme or spontaneous, wherein 10L is a formaldehyde dehydrogenase, wherein 10M is a S-(hydroxymethyl)glutathione synthase or spontaneous, wherein 10N is a glutathione-dependent formaldehyde dehydrogenase, wherein 10O is a S-formylglutathione hydrolase, wherein said FAACPE cycle comprises one or more β-ketoacyl-ACP synthase, one or more β-ketoacyl-ACP reductase, one or more β-hydroxyacyl-ACP reductase, and one or more enoyl ACP-reductase, wherein said termination pathway comprises a pathway selected from: (36) 12I; (37) 12J; (38) 12I, 12K, and 12L; (39) 12I and 12O; (40) 12J and 12M; (41) 12I, 12K, 12L, and 12M; (42) 12I, 12O, and 12M; (43) 12I, 12K and 12N, and (44) 12P, wherein 12I is a thioesterase, wherein 12J is a fatty acyl-ACP reductase, wherein 12K is an acyl-CoA synthase, wherein 12L is an acyl-CoA reductase, wherein 12M is a fatty aldehyde reductase, wherein 12N is a fatty alcohol forming acyl-CoA reductase (FAR), wherein 12O is a carboxylic acid reductase (CAR), wherein 12P is an acyl-ACP reductase (alcohol forming), wherein an enzyme of the formaldehyde fixation pathway, the formate assimilation pathway, the methanol metabolic pathway, the FAACPE cycle or the termination pathway is encoded by at least one exogenous nucleic acid and is expressed in a sufficient amount to produce a compound of Formula (I):

wherein R₁ is C₁₋₂₄ linear alkyl; R₂ is CH₂OH, CHO, or COOH; R₃ is H, OH, or oxo (═O); and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four, wherein the substrate of each of said enzymes of the FAACPE cycle and the termination pathway are independently selected from a compound of Formula (II) or malonyl-ACP:

wherein R₁ is C₁₋₂₄ linear alkyl; R₃ is H, OH, or oxo (═O); R₄ is S-CoA, ACP, OH or H; and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four; wherein said one or more enzymes of the FAACPE cycle are each selective for a compound of Formula (II) having a number of carbon atoms at R₁ that is no greater than the number of carbon atoms at R₁ of said compound of Formula (I), and wherein said one or more enzymes of the termination pathway are each selective for a compound of Formula (II) having a number of carbon atoms at R₁ that is no less than the number of carbon atoms at R₁ of said compound of Formula (I).

In some aspects of the invention, the microbial organism used in a method of the invention includes a non-naturally occurring having a combination of one or more pathways for generating substrates, intermediates and/or reducing equivalents that can be used with elongation cycles and termination pathways described herein for producing a fatty alcohol, fatty acid or fatty aldehyde of the invention. Accordingly, in some embodiments, the microbial organism has a formaldehyde fixation pathway and a MI-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formate assimilation pathway and a MI-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, and a MI-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway and a MD-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formate assimilation pathway and a MD-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, and a MD-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a methanol metabolic pathway and a MI-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a methanol metabolic pathway and a MD-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a methanol metabolic pathway and a MI-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formate assimilation pathway, a methanol metabolic pathway and a MI-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, a methanol metabolic pathway and a MI-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a methanol metabolic pathway and a MD-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formate assimilation pathway, a methanol metabolic pathway and a MD-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, a methanol metabolic pathway and MD-FAE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway and an FAACPE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formate assimilation pathway and an FAACPE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, and an FAACPE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a methanol metabolic pathway and an FAACPE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a methanol metabolic pathway and an FAACPE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formate assimilation pathway, a methanol metabolic pathway and an FAACPE cycle in combination with a termination pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, a methanol metabolic pathway and an FAACPE cycle in combination with a termination pathway.

In some aspects of the invention, the microbial organism used in a method of the invention that includes a FAACPE cycle in combination with a termination pathway as described herein, can further include a pathway for production of substrants for the FAACPE cycle, such as acetoacetyl-ACP or 3-oxovalery-ACP. Accordingly, in some embodiments, the microbial organism further comprises an acetoacetyl-ACP pathway of (1) 12A, 12B, and 12C; or (2) 12A, 12B, and 12D, wherein 12A is an acetyl-CoA carboxylase, wherein 12B is malonyl-CoA ACP transacylase, wherein 12C is an acetoacetyl-ACP synthase, and wherein 12D is a β-ketoacyl-ACP synthase. In some embodiments, the microbial organism further comprises a 3-oxovalery-ACP pathway comprising an acetyl-CoA carboxylase, a malonyl-CoA ACP transacylase, and a β-ketoacyl-ACP synthase. In some aspects of the invention, an enzyme of the acetoacetyl-ACP pathway is encoded by at least one exogenous nucleic acid and is expressed in a sufficient amount to produce acetoacetyl-ACP wherein the acetoacetyl-ACP is a β-ketoacyl-ACP of the FAACPE cycle. In some aspects of the invention, an enzyme of the 3-oxovalery-ACP pathway is encoded by at least one exogenous nucleic acid and is expressed in a sufficient amount to produce 3-oxovalery-ACP, wherein the 3-oxovalery-ACP is a β-ketoacyl-ACP of the FAACPE cycle.

In some embodiments, the invention provides a method for producing a compound of Formula (I) wherein R₁ is C₁₋₁₇ linear alkyl. In another aspect of the invention, the R₁ of the compound of Formula (I) is C₁ linear alkyl, C₂ linear alkyl, C₃ linear alkyl, C₄ linear alkyl, C₅ linear alkyl, C₆ linear alkyl, C₇ linear alkyl, C₈ linear alkyl, C₉ linear alkyl, C₁₀ linear alkyl, C₁₁, linear alkyl, C₁₂ linear alkyl or C₁₃ linear alkyl, C₁₄ linear alkyl, C₁₅ linear alkyl, C₁₆ linear alkyl, C₁₇ linear alkyl, C₁₈ linear alkyl, C₁₉ linear alkyl, C₂₀ linear alkyl, C₂₁ linear alkyl, C₂₂ linear alkyl, C₂₃ linear alkyl, or C₂₄ linear alkyl.

In some aspects of the invention, the microbial organism used in a method of the invention includes two, three, or four exogenous nucleic acids each encoding an enzyme of the MI-FAE cycle, the MD-FAE cycle, or the FAACPE cycle. In some aspects of the invention, the microbial organism includes two, three, or four exogenous nucleic acids each encoding an enzyme of the termination pathway. In some aspects of the invention, the microbial organism includes one, two, three, four, five, six, seven, or eight exogenous nucleic acids each encoding a formaldehyde fixation pathway enzyme, a formate assimilation pathway enzyme, or a methanol metabolic pathway enzyme. In some aspects of the invention, the microbial organism includes exogenous nucleic acids encoding each of the enzymes of at least one of the pathways selected from (1)-(49) for a microbial organism having a MI-FAE cycle or a MD-FAE cycle in combination with a termination pathway as depicted in FIGS. 1, 2, 7, 8 and 10. In some aspects of the invention, the microbial organism includes exogenous nucleic acids encoding each of the enzymes of at least one of the pathways selected from (1)-(44) for a microbial organism having a fatty acyl-ACP elongation (FAACPE) cycle in combination with a termination pathway as depicted in FIGS. 1, 10 and 12.

In some embodiments, the invention provides a method for producing a fatty alcohol selected from the Formulas (III)-(VI):

wherein R₁ is C₁₋₂₄ linear alkyl, or alternatively R₁ is C₁₋₁₇ linear alkyl, or alternatively R₁ is C₉₋₁₃ linear alkyl. In some aspects of the invention, R₁ is C₁ linear alkyl, C₂ linear alkyl, C₃ linear alkyl, C₄ linear alkyl, C₅ linear alkyl, C₆ linear alkyl, C₇ linear alkyl, C₈ linear alkyl, C₉ linear alkyl, C₁₀ linear alkyl, C₁₁, linear alkyl, C₁₂ linear alkyl or C₁₃ linear alkyl, C₁₄ linear alkyl, C₁₅ linear alkyl, C₁₆ linear alkyl, C₁₇ linear alkyl, C₁₈ linear alkyl, C₁₉ linear alkyl, C₂₀ linear alkyl, C₂₁ linear alkyl, C₂₂ linear alkyl, C₂₃ linear alkyl, or C₂₄ linear alkyl.

In some embodiments, the invention provides a method for producing a fatty aldehyde selected from the Formulas (VII)-(X):

wherein R₁ is C₁₋₂₄ linear alkyl, or alternatively R₁ is C₁₋₁₇ linear alkyl, or alternatively R₁ is C₉₋₁₃ linear alkyl. In some aspects of the invention, R₁ is C₁ linear alkyl, C₂ linear alkyl, C₃ linear alkyl, C₄ linear alkyl, C₅ linear alkyl, C₆ linear alkyl, C₇ linear alkyl, C₈ linear alkyl, C₉ linear alkyl, C₁₀ linear alkyl, C₁₁, linear alkyl, C₁₂ linear alkyl or C₁₃ linear alkyl, C₁₄ linear alkyl, C₁₅ linear alkyl, C₁₆ linear alkyl, C₁₇ linear alkyl, C₁₈ linear alkyl, C₁₉ linear alkyl, C₂₀ linear alkyl, C₂₁ linear alkyl, C₂₂ linear alkyl, C₂₃ linear alkyl, or C₂₄ linear alkyl.

In some embodiments, the invention provides a method for producing a fatty acid selected from the Formulas (XI)-(XIV):

wherein R₁ is C₁₋₂₄ linear alkyl, or alternatively R₁ is C₁₋₁₇ linear alkyl, or alternatively R₁ is C₉₋₁₃ linear alkyl. In some aspects of the invention, R₁ is C₁ linear alkyl, C₂ linear alkyl, C₃ linear alkyl, C₄ linear alkyl, C₅ linear alkyl, C₆ linear alkyl, C₇ linear alkyl, C₈ linear alkyl, C₉ linear alkyl, C₁₀ linear alkyl, C₁₁, linear alkyl, C₁₂ linear alkyl or C₁₃ linear alkyl, C₁₄ linear alkyl, C₁₅ linear alkyl, C₁₆ linear alkyl, C₁₇ linear alkyl, C₁₈ linear alkyl, C₁₉ linear alkyl, C₂₀ linear alkyl, C₂₁ linear alkyl, C₂₂ linear alkyl, C₂₃ linear alkyl, or C₂₄ linear alkyl.

In some embodiments, the invention provides a method for producing isopropanol comprising culturing the non-naturally occurring a microbial organism of the invention under conditions for a sufficient period of time to produce isopropanol.

In some aspects of the invention, the microbial organism used in a method of the invention includes a non-naturally occurring having: (i) a formaldehyde fixation pathway; (ii) a formate assimilation pathway; and/or (iii) a methanol metabolic pathway as depicted in FIGS. 1 and 10, and an isopropanol pathway as depicted in FIG. 11, wherein said formaldehyde fixation pathway comprises. (1) 1B and 1C; (2) 1D; or (3) 1D and 1Z, wherein 1B is a 3-hexulose-6-phosphate synthase, wherein 1C is a 6-phospho-3-hexuloisomerase, wherein 1D is a dihydroxyacetone synthase, wherein 1Z is a fructose-6-phosphate aldolase, wherein said formate assimilation pathway comprises a pathway selected from: (4) 1E; (5) 1F, and 1G; (6) 1H, 1I, 1J, and 1K; (7) 1H, 1I, 1J, 1L, 1M, and 1N; (8) 1E, 1H, 1I, 1J, 1L, 1M, and 1N; (9) 1F, 1G, 1H, 1I, 1J, 1L, 1M, and 1N; (10) 1K, 1H, 1I, 1J, 1L, 1M, and 1N; and (11) 1H, 1I, 1J, 1O, and 1P, wherein 1E is a formate reductase, 1F is a formate ligase, a formate transferase, or a formate synthetase, wherein 1G is a formyl-CoA reductase, wherein 1H is a formyltetrahydrofolate synthetase, wherein 1I is a methenyltetrahydrofolate cyclohydrolase, wherein 1J is a methylenetetrahydrofolate dehydrogenase, wherein 1K is a formaldehyde-forming enzyme or spontaneous, wherein 1L is a glycine cleavage system, wherein 1M is a serine hydroxymethyltransferase, wherein 1N is a serine deaminase, wherein 1O is a methylenetetrahydrofolate reductase, wherein 1P is an acetyl-CoA synthase, wherein said methanol metabolic pathway comprises a pathway selected from: (12) 10J; (13) 10A, (14) 10A and 10B; (15) 10A, 10B and 10C; (16) 10J, 10K and 10C; (17) 10J, 10M, and 10N; (18) 10J and 10L; (19) 10J, 10L and 10G; (20) 10J, 10L, and 10I; (21) 10A, 10B, 10C, 10D, and 10E; (22) 10A, 10B, 10C, 10D, and 10F; (23) 10J, 10K, 10C, 10D, and 10E; (24) 10J, 10K, 10C, 10D, and 10F; (25) 10J, 10M, 10N, and 10O; (26) 10A, 10B, 10C, 10D, 10E, and 10G; (27) 10A, 10B, 10C, 10D, 10F, and 10G; (28) 10J, 10K, 10C, 10D, 10E, and 10G; (29) 10J, 10K, 10C, 10D, 10F, and 10G; (30) 10J, 10M, 10N, 10O, and 10G; (31) 10A, 10B, 10C, 10D, 10E, and 10I; (32) 10A, 10B, 10C, 10D, 10F, and 10I; (33) 10J, 10K, 10C, 10D, 10E, and 10I; (34) 10J, 10K, 10C, 10D, 10F, and 10I; and (35) 10J, 10M, 10N, 10O, and 10I, wherein 10A is a methanol methyltransferase, wherein 10B is a methylenetetrahydrofolate reductase, wherein 10C is a methylenetetrahydrofolate dehydrogenase, wherein 10D is a methenyltetrahydrofolate cyclohydrolase, wherein 10E is a formyltetrahydrofolate deformylase, wherein 10F is a formyltetrahydrofolate synthetase, wherein 10G is a formate hydrogen lyase, wherein 10I is a formate dehydrogenase, wherein 10J is a methanol dehydrogenase, wherein 10K is a formaldehyde activating enzyme or spontaneous, wherein 10L is a formaldehyde dehydrogenase, wherein 10M is a S-(hydroxymethyl)glutathione synthase or spontaneous, wherein 10N is a glutathione-dependent formaldehyde dehydrogenase, wherein 10O is a S-formylglutathione hydrolase, wherein said isopanol pathway comprises. (36) 11V, 11W, 11X, and 11Y; or (37) 11T, 11U, 11W, 11X, and 11Y, wherein 11T is an acetyl-CoA carboxylase, wherein 11U is an acetoacetyl-CoA synthase, wherein 11V is an acetyl-CoA:acetyl-CoA acyltransferase, wherein 11W is an acetoacetyl-CoA hydrolase, an acetoacetyl-CoA transferase, an acetoacetyl-CoA ligase, or a phosphotransacetoacetylase/acetoacetate kinase, wherein 11X is an acetoacetate decarboxylase, wherein 11Y is an acetone reductase or isopropanol dehydrogenase, wherein an enzyme of the formaldehyde fixation pathway, formate assimilation pathway, methanol metabolic pathway, or isopropanol pathway is encoded by at least one exogenous nucleic acid and is expressed in a sufficient amount to produce isopropanol. In some embodiments, the non-naturally occurring microbial organism described herein comprises an acetyl-CoA pathway that comprises 1T and 1V and a formaldehyde fixation pathway that comprises 1D and 1Z. In some embodiments, the non-naturally occurring microbial organism described herein comprises an acetyl-CoA pathway that comprises 1T and 1V and a formaldehyde fixation pathway comprises 1B and 1C.

In some aspects of the invention, the microbial organism used in a method of the invention has a combination of one or more pathways for generating substrates, intermediates and/or reducing equivalents that can be used with isopropanol pathways described herein for producing isopropanol of the invention. Accordingly, in some embodiments, the microbial organism has a formaldehyde fixation pathway and an isopropanol pathway. In some embodiments, the microbial organism has a formate assimilation pathway and an isopropanol pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, and an isopropanol pathway. In some embodiments, the microbial organism has a methanol metabolic pathway and an isopropanol pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a methanol metabolic pathway and an isopropanol pathway. In some embodiments, the microbial organism has a formate assimilation pathway, a methanol metabolic pathway and an isopropanol pathway. In some embodiments, the microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, a methanol metabolic pathway and an isopropanol pathway.

In some aspects of the invention, the microbial organism used in a method of the invention includes two, three, four, five or six exogenous nucleic acids each encoding an enzyme of the isopropanol pathway. In some aspects of the invention, the microbial organism includes one, two, three, four, five, six, seven, or eight exogenous nucleic acids each encoding a formaldehyde fixation pathway enzyme, a formate assimilation pathway enzyme, or a methanol metabolic pathway enzyme. In some aspects of the invention, the microbial organism includes exogenous nucleic acids encoding each of the enzymes of at least one of the pathways selected from (1)-(37) for a microbial organism having an isopropanol pathway as depicted in FIGS. 1, 10 and 11.

In some aspects of the invention, the microbial organism used in a method of the invention having a formate assimilation pathway further includes wherein the formate assimilation pathway comprises. (1) 1Q; (2) 1R, and 1 S; (3) 1Y and 1Q; or (4) 1Y, 1R, and 1S, wherein 1Q is a pyruvate formate lyase, wherein 1R is a pyruvate dehydrogenase, a pyruvate ferredoxin oxidoreductase, or a pyruvate:NADP+ oxidoreductase, wherein 1S is a formate dehydrogenase wherein 1Y is a glyceraldehydes-3-phosphate dehydrogenase or an enzyme of lower glycolysis. In addition to a glyceraldehyde-3-phosphate dehydrogenase, lower glycolysis includes a phosphoglycerate kinase, a phosphoglyceromutase, an enolase, a pyruvate kinase or a PTS-dependant substrate import. Accordingly, in some embodiments, the formate assimilation pathway comprising 1Y includes an enzyme selected from a phosphoglycerate kinase, a phosphoglyceromutase, an enolase, a pyruvate kinase and a PTS-dependant substrate import.

In some aspects of the invention, the microbial organism used in a method of the invention includes a methanol oxidation pathway. Such a pathway can include at least one exogenous nucleic acid encoding a methanol oxidation pathway enzyme expressed in a sufficient amount to produce formaldehyde in the presence of methanol. An exemplary methanol oxidation pathway enzyme is a methanol dehydrogenase. Accordingly, in some aspects, the microbial organism used in the method of the invention includes a non-naturally occurring having at least one exogenous nucleic acid encoding a methanol dehydrogenase expressed in a sufficient amount to produce formaldehyde in the presence of methanol.

In some aspects of the invention, the microbial organism used in a method of the invention includes one or more enzymes for generating reducing equivalents. For example, the microbial organism can further include a hydrogenase and/or a carbon monoxide dehydrogenase. In some aspects, the microbial organism used in the method of the invention includes a non-naturally occurring having an exogenous nucleic acid encoding the hydrogenase or the carbon monoxide dehydrogenase.

In some aspects of the invention, the microbial organism used in a method of the invention includes a non-naturally occurring having at least one exogenous nucleic acid that is a heterologous nucleic acid. Accordingly, in some embodiments, the at least one exogenous nucleic acid encoding a formaldehyde fixation pathway enzyme described herein is a heterologous nucleic acid. In some embodiments, the at least one exogenous nucleic acid encoding a formate assimilation pathway enzyme described herein is a heterologous nucleic acid. In some embodiments, the at least one exogenous nucleic acid encoding a methanol metabolic pathway enzyme described herein is a heterologous nucleic acid. In some embodiments, the at least one exogenous nucleic acid encoding a MI-FAE cycle enzyme described herein is a heterologous nucleic acid. In some embodiments, the at least one exogenous nucleic acid encoding a MD-FAE cycle enzyme described herein is a heterologous nucleic acid. In some embodiments, the at least one exogenous nucleic acid encoding a FAACPE cycle enzyme described herein is a heterologous nucleic acid. In some embodiments, the at least one exogenous nucleic acid encoding a termination pathway enzyme described herein is a heterologous nucleic acid. In some embodiments, the at least one exogenous nucleic acid encoding an acetoacetyl-ACP pathway enzyme described herein is a heterologous nucleic acid. In some embodiments, the at least one exogenous nucleic acid encoding a 3-oxovalery-ACP pathway enzyme described herein is a heterologous nucleic acid. In some embodiments, the at least one exogenous nucleic acid encoding an isopropanol pathway enzyme described herein is a heterologous nucleic acid. In some embodiments, the at least one exogenous nucleic acid encoding a methanol oxidation pathway enzyme described herein is a heterologous nucleic acid. In some embodiments, the at least one exogenous nucleic acid encoding a hydrogenase or a carbon monoxide dehydrogenase is a heterologous nucleic acid.

In some embodiments, the method for producing a fatty alcohol, fatty aldehyde, fatty acid or isopropanol described herein includes using a non-naturally occurring microbial organism as described herein, wherein the microbial organism further includes an acetyl-CoA pathway and at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme expressed in a sufficient amount to produce or enhance carbon flux through acetyl-CoA, wherein the acetyl-CoA pathway includes a pathway shown in FIG. 1, 3, 4, 5 or 6 selected from: (1) 3A and 3B; (2) 3A, 3C, and 3D; (3) 3H; (4) 3G and 3D; (5) 3E, 3F and 3B; (6) 3E and 3I; (7) 3J, 3F and 3B; (8) 3J and 3I; (9) 4A, 4B, and 4C; (10) 4A, 4B, 4J, 4K, and 4D; (11) 4A, 4B, 4G, and 4D; (12) 4A, 4F, and 4D; (13) 4N, 4H, 4B and 4C; (14) 4N, 4H, 4B, 4J, 4K, and 4D; (15) 4N, 4H, 4B, 4G, and 4D; (16) 4N, 4H, 4F, and 4D; (17) 4L, 4M, 4B and 4C; (18) 4L, 4M, 4B, 4J, 4K, and 4D; (19) 4L, 4M, 4B, 4G, and 4D; (20) 4L, 4M, 4F, and 4D; (21) 5A, 5B, 5D, 5H, 5I, and 5J; (22) 5A, 5B, 5E, 5F, 5H, 5I, and 5J; (23) 5A, 5B, 5E, 5K, 5L, 5H, 5I, and 5J; (24) 5A, 5C, 5D, 5H, and 5J; (25) 5A, 5C, 5E, 5F, 5H, and 5J; (26) 5A, 5C, 5E, 5K, 5L, 5H, and 5J; (27) 6A, 6B, 6D, and 6G; (28) 6A, 6B, 6E, 6F, and 6G; (29) 6A, 6B, 6E, 6K, 6L, and 6G; (30) 6A, 6C, and 6D; (31) 6A, 6C, 6E, and 6F; (32) 6A, 6C, 6E, 6K, and 6L; (33) 1T and 1V; (34) 1T, 1W, and 1X; (35) 1U and 1V; and (36) 1U, 1W, and 1X, wherein 3A is a pyruvate oxidase (acetate-forming), wherein 3B is an acetyl-CoA synthetase, an acetyl-CoA ligase or an acetyl-CoA transferase, wherein 3C is an acetate kinase, wherein 3D is a phosphotransacetylase, wherein 3E is a pyruvate decarboxylase, wherein 3F is an acetaldehyde dehydrogenase, wherein 3G is a pyruvate oxidase (acetyl-phosphate forming), wherein 3H is a pyruvate dehydrogenase, a pyruvate:ferredoxin oxidoreductase, a pyruvate:NAD(P)H oxidoreductase or a pyruvate formate lyase, wherein 3I is an acetaldehyde dehydrogenase (acylating), wherein 3J is a threonine aldolase, wherein 4A is a phosphoenolpyruvate (PEP) carboxylase or a PEP carboxykinase, wherein 4B is an oxaloacetate decarboxylase, wherein 4C is a malonate semialdehyde dehydrogenase (acetylating), wherein 4D is an acetyl-CoA carboxylase or a malonyl-CoA decarboxylase, wherein 4F is an oxaloacetate dehydrogenase or an oxaloacetate oxidoreductase, wherein 4G is a malonate semialdehyde dehydrogenase (acylating), wherein 4H is a pyruvate carboxylase, wherein 4J is a malonate semialdehyde dehydrogenase, wherein 4K is a malonyl-CoA synthetase or a malonyl-CoA transferase, wherein 4L is a malic enzyme, wherein 4M is a malate dehydrogenase or a malate oxidoreductase, wherein 4N is a pyruvate kinase or a PEP phosphatase, wherein 5A is a citrate synthase, wherein 5B is a citrate transporter, wherein 5C is a citrate/malate transporter, wherein 5D is an ATP citrate lyase, wherein 5E is a citrate lyase, wherein 5F is an acetyl-CoA synthetase or an acetyl-CoA transferase, wherein 5H is a cytosolic malate dehydrogenase, wherein 5I is a malate transporter, wherein 5J is a mitochondrial malate dehydrogenase, wherein 5K is an acetate kinase, wherein 5L is a phosphotransacetylase, wherein 6A is a citrate synthase, wherein 6B is a citrate transporter, wherein 6C is a citrate/oxaloacetate transporter, wherein 6D is an ATP citrate lyase, wherein 6E is a citrate lyase, wherein 6F is an acetyl-CoA synthetase or an acetyl-CoA transferase, wherein 6G is an oxaloacetate transporter, wherein 6K is an acetate kinase, and wherein 6L is a phosphotransacetylase, wherein 1T is a fructose-6-phosphate phosphoketolase, wherein 1U is a xylulose-5-phosphate phosphoketolase, wherein 1V is a phosphotransacetylase, wherein 1W is an acetate kinase, wherein 1X is an acetyl-CoA transferase, an acetyl-CoA synthetase, or an acetyl-CoA ligase.

In some aspects, the microbial organism used in a method of the invention includes two, three, four, five, six, seven or eight exogenous nucleic acids each encoding an acetyl-CoA pathway enzyme. In some aspects, the microbial organism used in a method of the invention includes exogenous nucleic acids encoding each of the acetyl-CoA pathway enzymes of at least one of the pathways selected from (1)-(36).

In some aspects, the microbial organism used in a method of the invention includes further includes a propionyl-CoA pathway and at least one exogenous nucleic acid encoding a propionyl-CoA pathway enzyme expressed in a sufficient amount to produce propionyl-CoA, wherein the propionyl-CoA pathway includes a pathway shown in FIG. 22. For example, in some embodiments, the propionyl-CoA pathway comprises a pathway selected from: (1) 22A, 22E, 22F, 22G, 22I, 22J, 22K and 22L; (2) 22A, 22E, 22F, 22G, 22H, 22J, 22K and 22L; (3) 22B, 22E, 22F, 22G, 22I, 22J, 22K and 22L; (4) 22B, 22E, 22F, 22G, 22H, 22J, 22K and 22L; (5) 22C, 22D, 22E, 22F, 22G, 221, 22J, 22K and 22L; and (6) 22C, 22D, 22E, 22F, 22G, 22H, 22J, 22K and 22L, wherein 22A is a PEP carboxykinase, wherein 22B is a PEP carboxylase, wherein 22C is a Pyruvate kinase, wherein 22D is a Pyruvate carboxylase, wherein 22E is a Malate dehydrogenase, wherein 22F is a Fumarase, wherein 22G is a Fumarate reductase, wherein 22H is a Succinyl-CoA synthetase, wherein 22I is a Succinyl-CoA:3-ketoacid-CoA transferase, wherein 22J is a Methylmalonyl-CoA mutase, wherein 22K is a Methyl-malonyl-CoA epimerase, and wherein 22L is a Methylmalonyl-CoA decarboxylase.

In some embodiments, the invention provides a method for producing isopropanol, wherein the method includes culturing a non-naturally occurring microbial organism described herein under conditions and for a sufficient period of time to produce isopropanol, wherein the microbial organism comprises an acetyl-CoA pathway, wherein said acetyl-CoA pathway comprises a pathway selected from: (1) 1T and 1V; (2) 1T, 1W, and 1X; (3) 1U and 1V; (4) 1U, 1W, and 1X; wherein 1T is a fructose-6-phosphate phosphoketolase, wherein 1U is a xylulose-5-phosphate phosphoketolase, wherein 1V is a phosphotransacetylase, wherein 1W is an acetate kinase, wherein 1X is an acetyl-CoA transferase, an acetyl-CoA synthetase, or an acetyl-CoA ligase, wherein said non-naturally occurring microbial organism further comprises a pathway capable of producing isopropanol and an exogenous nucleic acid encoding an isopropanol pathway enzyme expressed in a sufficient amount to produce isopropanol, wherein said isopropanol pathway comprises a pathway selected from: (1) 11V, 11W, 11X, and 11Y; or (2) 11T, 11U, 11W, 11X, and 11Y, wherein 11T is an acetyl-CoA carboxylase, wherein 11U is an acetoacetyl-CoA synthase, wherein 11V is an acetyl-CoA:acetyl-CoA acyltransferase, wherein 11W is an acetoacetyl-CoA hydrolase, an acetoacetyl-CoA transferase, an acetoacetyl-CoA ligase, or a phosphotransacetoacetylase/acetoacetate kinase, wherein 11X is an acetoacetate decarboxylase, wherein 11Y is an acetone reductase or isopropanol dehydrogenase.

In other aspects, the invention further provides methods for producing elevated or enhanced yields of biosynthetic products such as a fatty alcohol, fatty aldehyde, fatty acid and/or isopropanol.

The methods for producing enhanced yields of a fatty alcohol, fatty aldehyde, fatty acid and/or isopropanol described herein include using a non-naturally occurring microbial organisms having one or more of the various pathway configurations employing a methanol dehydrogenase for methanol oxidation, a formaldehyde fixation pathway, and/or a phosphoketolase for directing the carbon from methanol into acetyl-CoA and other desired products via formaldehyde fixation as described previously. The methods include using a non-naturally occurring microbial organism of the invention having one or more of the various different methanol oxidation and formaldehyde fixation configurations exemplified previously and below engineered in conjunction with any or each of the various methanol oxidation, formaldehyde fixation, formate reutilization, fatty alcohol, fatty aldehyde, fatty acid and/or isopropanol pathway exemplified previously. Accordingly, the methods of the invention can use a microbial organism having one or more of the metabolic modifications exemplified previously and also below that increase biosynthetic product yields over, for example, endogenous methanol utilization pathways because they further focus methanol derived carbon into the assimilation pathways described herein, decrease inefficient use of methanol carbon through competing methanol utilization and/or formaldehyde fixation pathways and/or increase the production of reducing equivalents.

In some aspects, the methods of the invention can use microbial organisms containing or engineered to contain one or more of the various configurations of metabolic modifications disclosed herein for enhancing product yields via methanol derived carbon include enhancing methanol oxidation and production of reducing equivalents using either an endogenous NADH dependent methanol dehydrogenase, an exogenous NADH dependent methanol dehydrogenase, both an endogenous NADH dependent methanol dehydrogenase and exogenous NADH dependent methanol dehydrogenase alone or in combination with one or more metabolic modifications that attenuate, for example, DHA synthase and/or AOX. In addition, other metabolic modifications as exemplified previously and further below that reduce carbon flux away from methanol oxidation and formaldehyde fixation also can be included, alone or in combination, with the methanol oxidation and formaldehyde fixation pathway configurations disclosed herein that enhance carbon flux into product precursors such as acetyl-CoA and, therefore, enhance product yields.

Accordingly, in some embodiments, the microbial organisms used in a method of the invention can include one or more of any of the above and/or below metabolic modifications to a methanol utilization pathway and/or formaldehyde assimilation pathway configurations for enhancing product yields can be combined with any one or more, including all of the previously described methanol oxidation, formaldehyde fixation, formate reutilization, fatty alcohol, fatty aldehyde, fatty acid and/or isopropanol pathway to enhance the yield and/or production of a product such as any of the fatty alcohol, fatty aldehyde, fatty acids and/or isopropanol described herein.

Given the teachings and guidance provided herein, both prokaryotic and eukaryotic microbial organisms engineered to have methanol oxidation and/or formaldehyde fixation pathway configurations for enhancing product yields can be used in the methods of the invention. As exemplified herein and well known in the art, those skilled in the art will know which organism to select for a particular application. For example, with respect to eukaryotic microbial host organisms, those skilled in the art will know that yeasts and other eukaryotic microorganisms exhibit certain characteristics distinct from prokaryotic microbial organisms. When such characteristics are desirable, one skilled in the art can choose to use such eukaryotic microbial organisms having one or more of the various different methanol oxidation and formaldehyde fixation configurations exemplified herein for enhancing product yields in a method of the invention. Such characteristics have been described previously.

In some embodiments, the microbial organism used in a method of the invention and having a methanol oxidation and/or formaldehyde assimilation pathway configurations described herein for enhancing product yields can include, for example, a NADH-dependent methanol dehydrogenase (MeDH), one or more formaldehyde assimilation pathways and/or one or more phosphoketolases.

In one embodiment, the methods of the invention use microbial organisms that have cytosolic expression of one or more methanol oxidation and/or formaldehyde assimilation pathways. As described previously, exemplary pathways for converting cytosolic formaldehyde into glycolytic intermediates are shown in FIG. 1. Such pathways include methanol oxidation via expression of a cytosolic NADH dependent methanol dehydrogenase, formaldehyde fixation via expression of cytosolic DHA synthase, both methanol oxidation via expression of an cytosolic NADH dependent methanol dehydrogenase and formaldehyde fixation via expression of cytosolic DHA synthase alone or together with the metabolic modifications exemplified previously and also below that attenuate less beneficial methanol oxidation and/or formaldehyde fixation pathways. Such attenuating metabolic modifications include, for example, attenuation of alcohol oxidase, attenuation of DHA kinase and/or attenuation of DHA synthase (e.g. when ribulose-5-phosphate (Ru5P) pathway for formaldehyde fixation is utilized).

In another embodiment, conversion of cytosolic formaldehyde into glycolytic intermediates can occur via expression of a cytosolic 3-hexulose-6-phosphate (3-Hu6P) synthase. Thus, exemplary pathways that can be engineered into a microbial organism used in a method of the invention can include methanol oxidation via expression of a cytosolic NADH dependent methanol dehydrogenase, formaldehyde fixation via expression of cytosolic 3-Hu6P synthase, both methanol oxidation via expression of an cytosolic NADH dependent dehydrogenase and formaldehyde fixation via expression of cytosolic 3-Hu6P synthase alone or together with the metabolic modifications exemplified previously and also below that attenuate less beneficial methanol oxidation and/or formaldehyde fixation pathways. Such attenuating metabolic modifications include, for example, attenuation of alcohol oxidase, attenuation of DHA kinase and/or attenuation of DHA synthase (e.g. when ribulose-5-phosphate (Ru5P) pathway for formaldehyde fixation is utilized).

In yet another embodiment, the methods of the invention use microbial organisms that have cytosolic expression of one or more methanol oxidation and/or formaldehyde assimilation pathways. The formaldehyde assimilation pathways can include both assimilation through cytosolic DHA synthase and 3-Hu6P synthase. In this specific embodiment, such pathways include methanol oxidation via expression of a cytosolic NADH dependent methanol dehydrogenase, formaldehyde fixation via expression of cytosolic DHA synthase and 3-Hu6P synthase, both methanol oxidation via expression of an cytosolic NADH dependent dehydrogenase and formaldehyde fixation via expression of cytosolic DHA synthase and 3-Hu6P synthase alone or together with the metabolic modifications exemplified previously and also below that attenuate less beneficial methanol oxidation and/or formaldehyde fixation pathways. Such attenuating metabolic modifications include, for example, attenuation of alcohol oxidase, attenuation of DHA kinase and/or attenuation of DHA synthase (e.g. when ribulose-5-phosphate (Ru5P) pathway for formaldehyde fixation is utilized).

In some embodiments, the method for producing a fatty alcohol, fatty aldehyde, fatty acid or isopropanol described herein includes using a non-naturally occurring microbial organism as described herein, wherein the microbial organism further includes attenuation of one or more endogenous enzymes, which enhances carbon flux through acetyl-CoA. For example, in some aspects, the endogenous enzyme can be selected from DHA kinase, methanol oxidase, PQQ-dependent methanol dehydrogenase, DHA synthase or any combination thereof. Accordingly, in some aspects, the attenuation is of the endogenous enzyme DHA kinase. In some aspects, the attenuation is of the endogenous enzyme methanol oxidase. In some aspects, the attenuation is of the endogenous enzyme PQQ-dependent methanol dehydrogenase. In some aspects, the attenuation is of the endogenous enzyme DHA synthase. The invention also provides a method wherein the microbial organism used includes attenuation of any combination of two or three endogenous enzymes described herein. For example, a microbial organism can include attenuation of DHA kinase and DHA synthase, or alternatively methanol oxidase and PQQ-dependent methanol dehydrogenase, or alternatively DHA kinase, methanol oxidase, and PQQ-dependent methanol dehydrogenase, or alternatively DHA kinase, methanol oxidase, and DHA synthase. The invention also provides a method wherein the microbial organism used includes attenuation of all endogenous enzymes described herein. For example, in some aspects, a microbial organism includes attenuation of DHA kinase, methanol oxidase, PQQ-dependent methanol dehydrogenase and DHA synthase.

In some embodiments, the method for producing a fatty alcohol, fatty aldehyde, fatty acid or isopropanol described herein includes using a non-naturally occurring microbial organism as described herein, wherein the microbial organism further includes attenuation of one or more endogenous enzymes of a competing formaldehyde assimilation or dissimilation pathway. Examples of these endogenous enzymes are disclosed in FIG. 1 and described in Example XXIII. It is understood that a person skilled in the art would be able to readily identify enzymes of such competing pathways. Competing pathways can be dependent upon the host microbial organism and/or the exogenous nucleic acid introduced into the microbial organism as described herein. Accordingly, in some aspects of the invention, the method includes a microbial organism having attenuation of one, two, three, four, five, six, seven, eight, nine, ten or more endogenous enzymes of a competing formaldehyde assimilation or dissimilation pathway.

In some embodiments, the method for producing a fatty alcohol, fatty aldehyde, fatty acid or isopropanol described herein includes using a non-naturally occurring microbial organism as described herein, wherein the microbial organism further includes a gene disruption of one or more endogenous nucleic acids encoding enzymes, which enhances carbon flux through acetyl-CoA. For example, in some aspects, the endogenous enzyme can be selected from DHA kinase, methanol oxidase, PQQ-dependent methanol dehydrogenase, DHA synthase or any combination thereof. According, in some aspects, the gene disruption is of an endogenous nucleic acid encoding the enzyme DHA kinase. In some aspects, the gene disruption is of an endogenous nucleic acid encoding the enzyme methanol oxidase. In some aspects, the gene disruption is of an endogenous nucleic acid encoding the enzyme PQQ-dependent methanol dehydrogenase. In some aspects, the gene disruption is of an endogenous nucleic acid encoding the enzyme DHA synthase. The invention also provides a method wherein the microbial organism used includes the gene disruption of any combination of two or three nucleic acids encoding endogenous enzymes described herein. For example, a microbial organism of the invention can include a gene disruption of DHA kinase and DHA synthase, or alternatively methanol oxidase and PQQ-dependent methanol dehydrogenase, or alternatively DHA kinase, methanol oxidase, and PQQ-dependent methanol dehydrogenase, or alternatively DHA kinase, methanol oxidase, and DHA synthase. The invention also provides a method wherein the microbial organism used includes wherein all endogenous nucleic acids encoding enzymes described herein are disrupted. For example, in some aspects, a microbial organism described herein includes disruption of DHA kinase, methanol oxidase, PQQ-dependent methanol dehydrogenase and DHA synthase.

In some embodiments, the method for producing a fatty alcohol, fatty aldehyde, fatty acid or isopropanol described herein includes using a non-naturally occurring microbial organism as described herein, wherein the microbial organism further includes a gene disruption of one or more endogenous enzymes of a competing formaldehyde assimilation or dissimilation pathway. Examples of these endogenous enzymes are disclosed in FIG. 1 and described in Example XXIII. It is understood that a person skilled in the art would be able to readily identify enzymes of such competing pathways. Competing pathways can be dependent upon the host microbial organism and/or the exogenous nucleic acid introduced into the microbial organism as described herein. Accordingly, in some aspects of the invention, the microbial organism used in the method includes a gene disruption of one, two, three, four, five, six, seven, eight, nine, ten or more endogenous nucleic acids encoding enzymes of a competing formaldehyde assimilation or dissimilation pathway.

Suitable purification and/or assays to test for the production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol can be performed using well known methods. Suitable replicates such as triplicate cultures can be grown for each engineered strain to be tested. For example, product and byproduct formation in the engineered production host can be monitored. The final product and intermediates, and other organic compounds, can be analyzed by methods such as HPLC (High Performance Liquid Chromatography), GC-MS (Gas Chromatography-Mass Spectroscopy) and LC-MS (Liquid Chromatography-Mass Spectroscopy) or other suitable analytical methods using routine procedures well known in the art. The release of product in the fermentation broth can also be tested with the culture supernatant Byproducts and residual glucose can be quantified by HPLC using, for example, a refractive index detector for glucose and alcohols, and a UV detector for organic acids (Lin et al., Biotechnol. Bioeng. 90:775-779 (2005)), or other suitable assay and detection methods well known in the art. The individual enzyme or protein activities from the exogenous DNA sequences can also be assayed using methods well known in the art.

The fatty alcohol, fatty aldehyde, fatty acid or isopropanol can be separated from other components in the culture using a variety of methods well known in the art. Such separation methods include, for example, extraction procedures as well as methods that include continuous liquid-liquid extraction, pervaporation, membrane filtration, membrane separation, reverse osmosis, electrodialysis, distillation, crystallization, centrifugation, extractive filtration, ion exchange chromatography, size exclusion chromatography, adsorption chromatography, and ultrafiltration. All of the above methods are well known in the art.

Any of the non-naturally occurring microbial organisms described herein can be cultured to produce and/or secrete the biosynthetic products of the invention. For example, the fatty alcohol, fatty aldehyde, fatty acid or isopropanol producers can be cultured for the biosynthetic production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol. Accordingly, in some embodiments, the invention provides culture medium having the fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway intermediate described herein. In some aspects, the culture medium can also be separated from the non-naturally occurring microbial organisms of the invention that produced the fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway intermediate. Methods for separating a microbial organism from culture medium are well known in the art. Exemplary methods include filtration, flocculation, precipitation, centrifugation, sedimentation, and the like.

For the production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol, the recombinant strains are cultured in a medium with carbon source and other essential nutrients. It is sometimes desirable and can be highly desirable to maintain anaerobic conditions in the fermenter to reduce the cost of the overall process. Such conditions can be obtained, for example, by first sparging the medium with nitrogen and then sealing the flasks with a septum and crimp-cap. For strains where growth is not observed anaerobically, microaerobic or substantially anaerobic conditions can be applied by perforating the septum with a small hole for limited aeration. Exemplary anaerobic conditions have been described previously and are well-known in the art. Exemplary aerobic and anaerobic conditions are described, for example, in United State publication 2009/0047719, filed Aug. 10, 2007. Fermentations can be performed in a batch, fed-batch or continuous manner, as disclosed herein. Fermentations can also be conducted in two phases, if desired. The first phase can be aerobic to allow for high growth and therefore high productivity, followed by an anaerobic phase of high fatty alcohol, fatty aldehyde, fatty acid or isopropanol yields.

If desired, the pH of the medium can be maintained at a desired pH, in particular neutral pH, such as a pH of around 7 by addition of a base, such as NaOH or other bases, or acid, as needed to maintain the culture medium at a desirable pH. The growth rate can be determined by measuring optical density using a spectrophotometer (600 nm), and the glucose uptake rate by monitoring carbon source depletion over time.

The growth medium, can include, for example, any carbohydrate source which can supply a source of carbon to the non-naturally occurring microbial organism of the invention. Such sources include, for example, sugars such as glucose, xylose, arabinose, galactose, mannose, fructose, sucrose and starch; or glycerol, alone as the sole source of carbon or in combination with other carbon sources described herein or known in the art. In one embodiment, the carbon source is a sugar. In one embodiment, the carbon source is a sugar-containing biomass. In some embodiments, the sugar is glucose. In one embodiment, the sugar is xylose. In another embodiment, the sugar is arabinose. In one embodiment, the sugar is galactose. In another embodiment, the sugar is fructose. In other embodiments, the sugar is sucrose. In one embodiment, the sugar is starch. In certain embodiments, the carbon source is glycerol. In some embodiments, the carbon source is crude glycerol. In one embodiment, the carbon source is crude glycerol without treatment. In other embodiments, the carbon source is glycerol and glucose. In another embodiment, the carbon source is methanol and glycerol. In one embodiment, the carbon source is carbon dioxide. In one embodiment, the carbon source is formate. In one embodiment, the carbon source is methane. In one embodiment, the carbon source is methanol. In certain embodiments, methanol is used alone as the sole source of carbon or in combination with other carbon sources described herein or known in the art. In a specific embodiment, the methanol is the only (sole) carbon source. In one embodiment, the carbon source is chemoelectro-generated carbon (see, e.g., Liao et al. (2012) Science 335:1596). In one embodiment, the chemoelectro-generated carbon is methanol. In one embodiment, the chemoelectro-generated carbon is formate. In one embodiment, the chemoelectro-generated carbon is formate and methanol. In one embodiment, the carbon source is a carbohydrate and methanol. In one embodiment, the carbon source is a sugar and methanol. In another embodiment, the carbon source is a sugar and glycerol. In other embodiments, the carbon source is a sugar and crude glycerol. In yet other embodiments, the carbon source is a sugar and crude glycerol without treatment. In one embodiment, the carbon source is a sugar-containing biomass and methanol. In another embodiment, the carbon source is a sugar-containing biomass and glycerol. In other embodiments, the carbon source is a sugar-containing biomass and crude glycerol. In yet other embodiments, the carbon source is a sugar-containing biomass and crude glycerol without treatment. In some embodiments, the carbon source is a sugar-containing biomass, methanol and a carbohydrate. Other sources of carbohydrate include, for example, renewable feedstocks and biomass. Exemplary types of biomasses that can be used as feedstocks in the methods provided herein include cellulosic biomass, hemicellulosic biomass and lignin feedstocks or portions of feedstocks. Such biomass feedstocks contain, for example, carbohydrate substrates useful as carbon sources such as glucose, xylose, arabinose, galactose, mannose, fructose and starch. Given the teachings and guidance provided herein, those skilled in the art will understand that renewable feedstocks and biomass other than those exemplified above also can be used for culturing the microbial organisms provided herein for the production of succinate and other pathway intermediates.

In one embodiment, the carbon source is glycerol. In certain embodiments, the glycerol carbon source is crude glycerol or crude glycerol without further treatment. In a further embodiment, the carbon source comprises glycerol or crude glycerol, and also sugar or a sugar-containing biomass, such as glucose. In a specific embodiment, the concentration of glycerol in the fermentation broth is maintained by feeding crude glycerol, or a mixture of crude glycerol and sugar (e.g., glucose). In certain embodiments, sugar is provided for sufficient strain growth. In some embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of from 200:1 to 1:200. In some embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of from 100:1 to 1:100. In some embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of from 100:1 to 5:1. In some embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of from 50:1 to 5:1. In certain embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 100:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 90:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 80:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 70:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 60:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 50:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 40:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 30:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 20:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 10:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 5:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 2:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 1:1. In certain embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 1:100. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 1:90. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 1:80. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 1:70. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 1:60. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 1:50. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 1:40. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 1:30. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 1:20. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 1:10. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 1:5. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of glycerol to sugar of 1:2. In certain embodiments of the ratios provided above, the sugar is a sugar-containing biomass. In certain other embodiments of the ratios provided above, the glycerol is a crude glycerol or a crude glycerol without further treatment. In other embodiments of the ratios provided above, the sugar is a sugar-containing biomass, and the glycerol is a crude glycerol or a crude glycerol without further treatment.

Crude glycerol can be a by-product produced in the production of biodiesel, and can be used for fermentation without any further treatment. Biodiesel production methods include (1) a chemical method wherein the glycerol-group of vegetable oils or animal oils is substituted by low-carbon alcohols such as methanol or ethanol to produce a corresponding fatty acid methyl esters or fatty acid ethyl esters by transesterification in the presence of acidic or basic catalysts; (2) a biological method where biological enzymes or cells are used to catalyze transesterification reaction and the corresponding fatty acid methyl esters or fatty acid ethyl esters are produced; and (3) a supercritical method, wherein transesterification reaction is carried out in a supercritical solvent system without any catalysts. The chemical composition of crude glycerol can vary with the process used to produce biodiesel, the transesterification efficiency, recovery efficiency of the biodiesel, other impurities in the feedstock, and whether methanol and catalysts were recovered. For example, the chemical compositions of eleven crude glycerol collected from seven Australian biodiesel producers reported that glycerol content ranged between 38% and 96%, with some samples including more than 14% methanol and 29% ash. In certain embodiments, the crude glycerol comprises from 5% to 99% glycerol. In some embodiments, the crude glycerol comprises from 10% to 90% glycerol. In some embodiments, the crude glycerol comprises from 10% to 80% glycerol. In some embodiments, the crude glycerol comprises from 10% to 70% glycerol. In some embodiments, the crude glycerol comprises from 10% to 60% glycerol. In some embodiments, the crude glycerol comprises from 10% to 50% glycerol. In some embodiments, the crude glycerol comprises from 10% to 40% glycerol. In some embodiments, the crude glycerol comprises from 10% to 30% glycerol. In some embodiments, the crude glycerol comprises from 10% to 20% glycerol. In some embodiments, the crude glycerol comprises from 80% to 90% glycerol. In some embodiments, the crude glycerol comprises from 70% to 90% glycerol. In some embodiments, the crude glycerol comprises from 60% to 90% glycerol. In some embodiments, the crude glycerol comprises from 50% to 90% glycerol. In some embodiments, the crude glycerol comprises from 40% to 90% glycerol. In some embodiments, the crude glycerol comprises from 30% to 90% glycerol. In some embodiments, the crude glycerol comprises from 20% to 90% glycerol. In some embodiments, the crude glycerol comprises from 20% to 40% glycerol. In some embodiments, the crude glycerol comprises from 40% to 60% glycerol. In some embodiments, the crude glycerol comprises from 60% to 80% glycerol. In some embodiments, the crude glycerol comprises from 50% to 70% glycerol. In one embodiment, the glycerol comprises 5% glycerol. In one embodiment, the glycerol comprises 10% glycerol. In one embodiment, the glycerol comprises 15% glycerol. In one embodiment, the glycerol comprises 20% glycerol. In one embodiment, the glycerol comprises 25% glycerol. In one embodiment, the glycerol comprises 30% glycerol. In one embodiment, the glycerol comprises 35% glycerol. In one embodiment, the glycerol comprises 40% glycerol. In one embodiment, the glycerol comprises 45% glycerol. In one embodiment, the glycerol comprises 50% glycerol. In one embodiment, the glycerol comprises 55% glycerol. In one embodiment, the glycerol comprises 60% glycerol. In one embodiment, the glycerol comprises 65% glycerol. In one embodiment, the glycerol comprises 70% glycerol. In one embodiment, the glycerol comprises 75% glycerol. In one embodiment, the glycerol comprises 80% glycerol. In one embodiment, the glycerol comprises 85% glycerol. In one embodiment, the glycerol comprises 90% glycerol. In one embodiment, the glycerol comprises 95% glycerol. In one embodiment, the glycerol comprises 99% glycerol.

In one embodiment, the carbon source is methanol or formate. In certain embodiments, methanol is used as a carbon source in a formaldehyde fixation pathway provided herein. In one embodiment, the carbon source is methanol or formate. In other embodiments, formate is used as a carbon source in a formaldehyde fixation pathway provided herein. In specific embodiments, methanol is used as a carbon source in a methanol oxidation pathway provided herein, either alone or in combination with the fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathways provided herein. In one embodiment, the carbon source is methanol. In another embodiment, the carbon source is formate.

In one embodiment, the carbon source comprises methanol, and sugar (e.g., glucose) or a sugar-containing biomass. In another embodiment, the carbon source comprises formate, and sugar (e.g., glucose) or a sugar-containing biomass. In one embodiment, the carbon source comprises methanol, formate, and sugar (e.g., glucose) or a sugar-containing biomass. In specific embodiments, the methanol or formate, or both, in the fermentation feed is provided as a mixture with sugar (e.g., glucose) or sugar-comprising biomass. In certain embodiments, sugar is provided for sufficient strain growth.

In certain embodiments, the carbon source comprises methanol and a sugar (e.g., glucose). In some embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of from 200:1 to 1:200. In some embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of from 100:1 to 1:100. In some embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of from 100:1 to 5:1. In some embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of from 50:1 to 5:1. In certain embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 100:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 90:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 80:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 70:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 60:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 50:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 40:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 30:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 20:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 10:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 5:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 2:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 1:1. In certain embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 1:100. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 1:90. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 1:80. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 1:70. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 1:60. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 1:50. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 1:40. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 1:30. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 1:20. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 1:10. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 1:5. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol to sugar of 1:2. In certain embodiments of the ratios provided above, the sugar is a sugar-containing biomass.

In certain embodiments, the carbon source comprises formate and a sugar (e.g., glucose). In some embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of from 200:1 to 1:200. In some embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of from 100:1 to 1:100. In some embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of from 100:1 to 5:1. In some embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of from 50:1 to 5:1. In certain embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 100:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 90:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 80:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 70:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 60:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 50:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 40:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 30:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 20:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 10:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 5:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 2:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 1:1. In certain embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 1:100. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 1:90. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 1:80. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 1:70. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 1:60. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 1:50. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 1:40. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 1:30. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 1:20. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 1:10. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 1:5. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of formate to sugar of 1:2. In certain embodiments of the ratios provided above, the sugar is a sugar-containing biomass.

In certain embodiments, the carbon source comprises a mixture of methanol and formate, and a sugar (e.g., glucose). In certain embodiments, sugar is provided for sufficient strain growth. In some embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of from 200:1 to 1:200. In some embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of from 100:1 to 1:100. In some embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of from 100:1 to 5:1. In some embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of from 50:1 to 5:1. In certain embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 100:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 90:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 80:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 70:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 60:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 50:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 40:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 30:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 20:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 10:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 5:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 2:1. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 1:1. In certain embodiments, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 1:100. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 1:90. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 1:80. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 1:70. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 1:60. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 1:50. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 1:40. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 1:30. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 1:20. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 1:10. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 1:5. In one embodiment, the sugar (e.g., glucose) is provided at a molar concentration ratio of methanol and formate to sugar of 1:2. In certain embodiments of the ratios provided above, the sugar is a sugar-containing biomass.

In addition to renewable feedstocks such as those exemplified above, the fatty alcohol, fatty aldehyde, fatty acid or isopropanol producing microbial organisms of the invention also can be modified for growth on syngas as its source of carbon. In this specific embodiment, one or more proteins or enzymes are expressed in the fatty alcohol, fatty aldehyde, fatty acid or isopropanol producing organisms to provide a metabolic pathway for utilization of syngas or other gaseous carbon source.

Synthesis gas, also known as syngas or producer gas, is the major product of gasification of coal and of carbonaceous materials such as biomass materials, including agricultural crops and residues. Syngas is a mixture primarily of H₂ and CO and can be obtained from the gasification of any organic feedstock, including but not limited to coal, coal oil, natural gas, biomass, and waste organic matter. Gasification is generally carried out under a high fuel to oxygen ratio. Although largely H₂ and CO, syngas can also include CO₂ and other gases in smaller quantities. Thus, synthesis gas provides a cost effective source of gaseous carbon such as CO and, additionally, CO₂.

Accordingly, given the teachings and guidance provided herein, those skilled in the art will understand that a non-naturally occurring microbial organism can be produced that secretes the biosynthesized compounds of the invention when grown on a carbon source such as a carbohydrate. Such compounds include, for example, fatty alcohol, fatty aldehyde, fatty acid or isopropanol and any of the intermediate metabolites in the fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway. All that is required is to engineer in one or more of the required enzyme or protein activities to achieve biosynthesis of the desired compound or intermediate including, for example, inclusion of some or all of the fatty alcohol, fatty aldehyde, fatty acid or isopropanol biosynthetic pathways. Accordingly, the invention provides a non-naturally occurring microbial organism that produces and/or secretes fatty alcohol, fatty aldehyde, fatty acid or isopropanol when grown on a carbohydrate or other carbon source and produces and/or secretes any of the intermediate metabolites shown in the fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway when grown on a carbohydrate or other carbon source. The fatty alcohol, fatty aldehyde, fatty acid or isopropanol producing microbial organisms of the invention can initiate synthesis from an intermediate, for example, a 3-ketoacyl-CoA, a 3-hydroxyacyl-CoA, an enoyl-CoA, an acyl-CoA, an acyl-ACP, acetate, acetaldehyde, acetyl-phosphate, oxaloacetate, matate, malonate semialdehyde, malonate, malonyl-CoA, acetyl-CoA, or citrate.

The non-naturally occurring microbial organisms of the invention are constructed using methods well known in the art as exemplified herein to exogenously express at least one nucleic acid encoding a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway enzyme or protein in sufficient amounts to produce fatty alcohol, fatty aldehyde, fatty acid or isopropanol. It is understood that the microbial organisms of the invention are cultured under conditions sufficient to produce fatty alcohol, fatty aldehyde, fatty acid or isopropanol. Following the teachings and guidance provided herein, the non-naturally occurring microbial organisms of the invention can achieve biosynthesis of fatty alcohol, fatty aldehyde, fatty acid or isopropanol resulting in intracellular concentrations between about 0.1-200 mM or more. Generally, the intracellular concentration of fatty alcohol, fatty aldehyde, fatty acid or isopropanol is between about 3-150 mM, particularly between about 5-125 mM and more particularly between about 8-100 mM, including about 10 mM, 20 mM, 50 mM, 80 mM, or more. Intracellular concentrations between and above each of these exemplary ranges also can be achieved from the non-naturally occurring microbial organisms of the invention.

In some embodiments, culture conditions include anaerobic or substantially anaerobic growth or maintenance conditions. Exemplary anaerobic conditions have been described previously and are well known in the art. Exemplary anaerobic conditions for fermentation processes are described herein and are described, for example, in U.S. publication 2009/0047719, filed Aug. 10, 2007. Any of these conditions can be employed with the non-naturally occurring microbial organisms as well as other anaerobic conditions well known in the art. Under such anaerobic or substantially anaerobic conditions, the fatty alcohol, fatty aldehyde, fatty acid or isopropanol producers can synthesize fatty alcohol, fatty aldehyde, fatty acid or isopropanol at intracellular concentrations of 5-10 mM or more as well as all other concentrations exemplified herein. It is understood that, even though the above description refers to intracellular concentrations, fatty alcohol, fatty aldehyde, fatty acid or isopropanol producing microbial organisms can produce fatty alcohol, fatty aldehyde, fatty acid or isopropanol intracellularly and/or secrete the product into the culture medium.

Exemplary fermentation processes include, but are not limited to, fed-batch fermentation and batch separation; fed-batch fermentation and continuous separation; and continuous fermentation and continuous separation. In an exemplary batch fermentation protocol, the production organism is grown in a suitably sized bioreactor sparged with an appropriate gas. Under anaerobic conditions, the culture is sparged with an inert gas or combination of gases, for example, nitrogen, N₂/CO₂ mixture, argon, helium, and the like. As the cells grow and utilize the carbon source, additional carbon source(s) and/or other nutrients are fed into the bioreactor at a rate approximately balancing consumption of the carbon source and/or nutrients. The temperature of the bioreactor is maintained at a desired temperature, generally in the range of 22-37 degrees C., but the temperature can be maintained at a higher or lower temperature depending on the growth characteristics of the production organism and/or desired conditions for the fermentation process. Growth continues for a desired period of time to achieve desired characteristics of the culture in the fermenter, for example, cell density, product concentration, and the like. In a batch fermentation process, the time period for the fermentation is generally in the range of several hours to several days, for example, 8 to 24 hours, or 1, 2, 3, 4 or 5 days, or up to a week, depending on the desired culture conditions. The pH can be controlled or not, as desired, in which case a culture in which pH is not controlled will typically decrease to pH 3-6 by the end of the run. Upon completion of the cultivation period, the fermenter contents can be passed through a cell separation unit, for example, a centrifuge, filtration unit, and the like, to remove cells and cell debris. In the case where the desired product is expressed intracellularly, the cells can be lysed or disrupted enzymatically or chemically prior to or after separation of cells from the fermentation broth, as desired, in order to release additional product. The fermentation broth can be transferred to a product separations unit Isolation of product occurs by standard separations procedures employed in the art to separate a desired product from dilute aqueous solutions. Such methods include, but are not limited to, liquid-liquid extraction using a water immiscible organic solvent (e.g., toluene or other suitable solvents, including but not limited to diethyl ether, ethyl acetate, tetrahydrofuran (THF), methylene chloride, chloroform, benzene, pentane, hexane, heptane, petroleum ether, methyl tertiary butyl ether (MTBE), dioxane, dimethylformamide (DMF), dimethyl sulfoxide (DMSO), and the like) to provide an organic solution of the product, if appropriate, standard distillation methods, and the like, depending on the chemical characteristics of the product of the fermentation process.

In an exemplary fully continuous fermentation protocol, the production organism is generally first grown up in batch mode in order to achieve a desired cell density. When the carbon source and/or other nutrients are exhausted, feed medium of the same composition is supplied continuously at a desired rate, and fermentation liquid is withdrawn at the same rate. Under such conditions, the product concentration in the bioreactor generally remains constant, as well as the cell density. The temperature of the fermenter is maintained at a desired temperature, as discussed above. During the continuous fermentation phase, it is generally desirable to maintain a suitable pH range for optimized production. The pH can be monitored and maintained using routine methods, including the addition of suitable acids or bases to maintain a desired pH range. The bioreactor is operated continuously for extended periods of time, generally at least one week to several weeks and up to one month, or longer, as appropriate and desired. The fermentation liquid and/or culture is monitored periodically, including sampling up to every day, as desired, to assure consistency of product concentration and/or cell density. In continuous mode, fermenter contents are constantly removed as new feed medium is supplied. The exit stream, containing cells, medium, and product, are generally subjected to a continuous product separations procedure, with or without removing cells and cell debris, as desired. Continuous separations methods employed in the art can be used to separate the product from dilute aqueous solutions, including but not limited to continuous liquid-liquid extraction using a water immiscible organic solvent (e.g., toluene or other suitable solvents, including but not limited to diethyl ether, ethyl acetate, tetrahydrofuran (THF), methylene chloride, chloroform, benzene, pentane, hexane, heptane, petroleum ether, methyl tertiary butyl ether (MTBE), dioxane, dimethylformamide (DMF), dimethyl sulfoxide (DMSO), and the like), standard continuous distillation methods, and the like, or other methods well known in the art.

In addition to the culturing and fermentation conditions disclosed herein, growth condition for achieving biosynthesis of fatty alcohol, fatty aldehyde, fatty acid or isopropanol can include the addition of an osmoprotectant to the culturing conditions. In certain embodiments, the non-naturally occurring microbial organisms of the invention can be sustained, cultured or fermented as described herein in the presence of an osmoprotectant. Briefly, an osmoprotectant refers to a compound that acts as an osmolyte and helps a microbial organism as described herein survive osmotic stress. Osmoprotectants include, but are not limited to, betaines, amino acids, and the sugar trehalose. Non-limiting examples of such are glycine betaine, praline betaine, dimethylthetin, dimethylslfonioproprionate, 3-dimethylsulfonio-2-methylproprionate, pipecolic acid, dimethylsulfonioacetate, choline, L-carnitine and ectoine. In one aspect, the osmoprotectant is glycine betaine. It is understood to one of ordinary skill in the art that the amount and type of osmoprotectant suitable for protecting a microbial organism described herein from osmotic stress will depend on the microbial organism used. The amount of osmoprotectant in the culturing conditions can be, for example, no more than about 0.1 mM, no more than about 0.5 mM, no more than about 1.0 mM, no more than about 1.5 mM, no more than about 2.0 mM, no more than about 2.5 mM, no more than about 3.0 mM, no more than about 5.0 mM, no more than about 7.0 mM, no more than about 10 mM, no more than about 50 mM, no more than about 100 mM or no more than about 500 mM.

In some embodiments, the carbon feedstock and other cellular uptake sources such as phosphate, ammonia, sulfate, chloride and other halogens can be chosen to alter the isotopic distribution of the atoms present in fatty alcohol, fatty aldehyde, fatty acid or isopropanol or any fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway intermediate. The various carbon feedstock and other uptake sources enumerated above will be referred to herein, collectively, as “uptake sources.” Uptake sources can provide isotopic enrichment for any atom present in the product fatty alcohol, fatty aldehyde, fatty acid or isopropanol or fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway intermediate, or for side products generated in reactions diverging away from a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway. Isotopic enrichment can be achieved for any target atom including, for example, carbon, hydrogen, oxygen, nitrogen, sulfur, phosphorus, chloride or other halogens.

In some embodiments, the uptake sources can be selected to alter the carbon-12, carbon-13, and carbon-14 ratios. In some embodiments, the uptake sources can be selected to alter the oxygen-16, oxygen-17, and oxygen-18 ratios. In some embodiments, the uptake sources can be selected to alter the hydrogen, deuterium, and tritium ratios. In some embodiments, the uptake sources can be selected to alter the nitrogen-14 and nitrogen-15 ratios. In some embodiments, the uptake sources can be selected to alter the sulfur-32, sulfur-33, sulfur-34, and sulfur-35 ratios. In some embodiments, the uptake sources can be selected to alter the phosphorus-31, phosphorus-32, and phosphorus-33 ratios. In some embodiments, the uptake sources can be selected to alter the chlorine-35, chlorine-36, and chlorine-37 ratios.

In some embodiments, the isotopic ratio of a target atom can be varied to a desired ratio by selecting one or more uptake sources. An uptake source can be derived from a natural source, as found in nature, or from a man-made source, and one skilled in the art can select a natural source, a man-made source, or a combination thereof, to achieve a desired isotopic ratio of a target atom. An example of a man-made uptake source includes, for example, an uptake source that is at least partially derived from a chemical synthetic reaction. Such isotopically enriched uptake sources can be purchased commercially or prepared in the laboratory and/or optionally mixed with a natural source of the uptake source to achieve a desired isotopic ratio. In some embodiments, a target atom isotopic ratio of an uptake source can be achieved by selecting a desired origin of the uptake source as found in nature. For example, as discussed herein, a natural source can be a biobased derived from or synthesized by a biological organism or a source such as petroleum-based products or the atmosphere. In some such embodiments, a source of carbon, for example, can be selected from a fossil fuel-derived carbon source, which can be relatively depleted of carbon-14, or an environmental or atmospheric carbon source, such as CO₂, which can possess a larger amount of carbon-14 than its petroleum-derived counterpart.

The unstable carbon isotope carbon-14 or radiocarbon makes up for roughly 1 in 10¹² carbon atoms in the earth's atmosphere and has a half-life of about 5700 years. The stock of carbon is replenished in the upper atmosphere by a nuclear reaction involving cosmic rays and ordinary nitrogen (¹⁴N) Fossil fuels contain no carbon-14, as it decayed long ago. Burning of fossil fuels lowers the atmospheric carbon-14 fraction, the so-called “Suess effect”.

Methods of determining the isotopic ratios of atoms in a compound are well known to those skilled in the art. Isotopic enrichment is readily assessed by mass spectrometry using techniques known in the art such as accelerated mass spectrometry (AMS), Stable Isotope Ratio Mass Spectrometry (SIRMS) and Site-Specific Natural Isotopic Fractionation by Nuclear Magnetic Resonance (SNIF-NMR). Such mass spectral techniques can be integrated with separation techniques such as liquid chromatography (LC), high performance liquid chromatography (HPLC) and/or gas chromatography, and the like.

In the case of carbon, ASTM D6866 was developed in the United States as a standardized analytical method for determining the biobased content of solid, liquid, and gaseous samples using radiocarbon dating by the American Society for Testing and Materials (ASTM) International. The standard is based on the use of radiocarbon dating for the determination of a product's biobased content ASTM D6866 was first published in 2004, and the current active version of the standard is ASTM D6866-11 (effective Apr. 1, 2011). Radiocarbon dating techniques are well known to those skilled in the art, including those described herein.

The biobased content of a compound is estimated by the ratio of carbon-14 (¹⁴C) to carbon-12 (¹²C). Specifically, the Fraction Modern (Fm) is computed from the expression: Fm=(S−B)/(M−B), where B, S and M represent the ¹⁴C/¹²C ratios of the blank, the sample and the modern reference, respectively. Fraction Modern is a measurement of the deviation of the ¹⁴C/¹²C ratio of a sample from “Modern.” Modern is defined as 95% of the radiocarbon concentration (in AD 1950) of National Bureau of Standards (NBS) Oxalic Acid I (i.e., standard reference materials (SRM) 4990b) normalized to δ¹³C_(VPDB)=−19 per mil (Olsson, The use of Oxalic acid as a Standard. in, Radiocarbon Variations and Absolute Chronology, Nobel Symposium, 12th Proc., John Wiley & Sons, New York (1970)). Mass spectrometry results, for example, measured by ASM, are calculated using the internationally agreed upon definition of 0.95 times the specific activity of NBS Oxalic Acid I (SRM 4990b) normalized to δ¹³C_(VPDB)=−19 per mil. This is equivalent to an absolute (AD 1950)¹⁴C/¹²C ratio of 1.176±0.010×10⁻¹² (Karlen et al., Arkiv Geoftsik, 4:465-471 (1968)). The standard calculations take into account the differential uptake of one isotope with respect to another, for example, the preferential uptake in biological systems of C¹² over C¹³ over C¹⁴, and these corrections are reflected as a Fm corrected for δ¹³.

An oxalic acid standard (SRM 4990b or HOx 1) was made from a crop of 1955 sugar beet. Although there were 1000 lbs made, this oxalic acid standard is no longer commercially available. The Oxalic Acid II standard (HOx 2; N.I.S.T designation SRM 4990 C) was made from a crop of 1977 French beet molasses. In the early 1980's, a group of 12 laboratories measured the ratios of the two standards. The ratio of the activity of Oxalic acid II to 1 is 1.2933±0.001 (the weighted mean). The isotopic ratio of HOx II is −17.8 per mil. ASTM D6866-11 suggests use of the available Oxalic Acid II standard SRM 4990 C (Hox2) for the modern standard (see discussion of original vs. currently available oxalic acid standards in Mann, Radiocarbon, 25(2):519-527 (1983)). A Fm=0% represents the entire lack of carbon-14 atoms in a material, thus indicating a fossil (for example, petroleum based) carbon source. A Fm=100%, after correction for the post-1950 injection of carbon-14 into the atmosphere from nuclear bomb testing, indicates an entirely modern carbon source. As described herein, such a “modern” source includes biobased sources.

As described in ASTM D6866, the percent modern carbon (pMC) can be greater than 100% because of the continuing but diminishing effects of the 1950s nuclear testing programs, which resulted in a considerable enrichment of carbon-14 in the atmosphere as described in ASTM D6866-11. Because all sample carbon-14 activities are referenced to a “pre-bomb” standard, and because nearly all new biobased products are produced in a post-bomb environment, all pMC values (after correction for isotopic fraction) must be multiplied by 0.95 (as of 2010) to better reflect the true biobased content of the sample. A biobased content that is greater than 103% suggests that either an analytical error has occurred, or that the source of biobased carbon is more than several years old.

ASTM D6866 quantifies the biobased content relative to the material's total organic content and does not consider the inorganic carbon and other non-carbon containing substances present. For example, a product that is 50% starch-based material and 50% water would be considered to have a Biobased Content=100% (50% organic content that is 100% biobased) based on ASTM D6866. In another example, a product that is 50% starch-based material, 25% petroleum-based, and 25% water would have a Biobased Content=66.7% (75% organic content but only 50% of the product is biobased). In another example, a product that is 50% organic carbon and is a petroleum-based product would be considered to have a Biobased Content=0% (50% organic carbon but from fossil sources). Thus, based on the well known methods and known standards for determining the biobased content of a compound or material, one skilled in the art can readily determine the biobased content and/or prepared downstream products that utilize of the invention having a desired biobased content.

Applications of carbon-14 dating techniques to quantify bio-based content of materials are known in the art (Currie et al., Nuclear Instruments and Methods in Physics Research B, 172:281-287 (2000)). For example, carbon-14 dating has been used to quantify bio-based content in terephthalate-containing materials (Colonna et al., Green Chemistry, 13:2543-2548 (2011)). Notably, polypropylene terephthalate (PPT) polymers derived from renewable 1,3-propanediol and petroleum-derived terephthalic acid resulted in Fm values near 30% (i.e., since 3/11 of the polymeric carbon derives from renewable 1,3-propanediol and 8/11 from the fossil end member terephthalic acid) (Currie et al., supra, 2000). In contrast, polybutylene terephthalate polymer derived from both renewable 1,4-butanediol and renewable terephthalic acid resulted in bio-based content exceeding 90% (Colonna et al., supra, 2011).

Accordingly, in some embodiments, the present invention provides fatty alcohol, fatty aldehyde, fatty acid or isopropanol or a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway intermediate that has a carbon-12, carbon-13, and carbon-14 ratio that reflects an atmospheric carbon, also referred to as environmental carbon, uptake source. For example, in some aspects the fatty alcohol, fatty aldehyde, fatty acid or isopropanol or a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway intermediate can have an Fm value of at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98% or as much as 100%. In some such embodiments, the uptake source is CO₂. In some embodiments, the present invention provides fatty alcohol, fatty aldehyde, fatty acid or isopropanol or a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway intermediate that has a carbon-12, carbon-13, and carbon-14 ratio that reflects petroleum-based carbon uptake source. In this aspect, the fatty alcohol, fatty aldehyde, fatty acid or isopropanol or a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway intermediate can have an Fm value of less than 95%, less than 90%, less than 85%, less than 80%, less than 75%, less than 70%, less than 65%, less than 60%, less than 55%, less than 50%, less than 45%, less than 40%, less than 35%, less than 30%, less than 25%, less than 20%, less than 15%, less than 10%, less than 5%, less than 2% or less than 1%. In some embodiments, the present invention provides fatty alcohol, fatty aldehyde, fatty acid or isopropanol or a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway intermediate that has a carbon-12, carbon-13, and carbon-14 ratio that is obtained by a combination of an atmospheric carbon uptake source with a petroleum-based uptake source. Using such a combination of uptake sources is one way by which the carbon-12, carbon-13, and carbon-14 ratio can be varied, and the respective ratios would reflect the proportions of the uptake sources.

Further, the present invention relates to the biologically produced fatty alcohol, fatty aldehyde, fatty acid or isopropanol or fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway intermediate as disclosed herein, and to the products derived therefrom, wherein the fatty alcohol, fatty aldehyde, fatty acid or isopropanol or a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway intermediate has a carbon-12, carbon-13, and carbon-14 isotope ratio of about the same value as the CO₂ that occurs in the environment. For example, in some aspects the invention provides bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol or a bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol intermediate having a carbon-12 versus carbon-13 versus carbon-14 isotope ratio of about the same value as the CO₂ that occurs in the environment, or any of the other ratios disclosed herein. It is understood, as disclosed herein, that a product can have a carbon-12 versus carbon-13 versus carbon-14 isotope ratio of about the same value as the CO₂ that occurs in the environment, or any of the ratios disclosed herein, wherein the product is generated from bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol or a bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway intermediate as disclosed herein, wherein the bioderived product is chemically modified to generate a final product. Methods of chemically modifying a bioderived product of fatty alcohol, fatty aldehyde, fatty acid or isopropanol, or an intermediate thereof, to generate a desired product are well known to those skilled in the art, as described herein. The invention further provides biofuels, chemicals, polymers, surfactants, soaps, detergents, shampoos, lubricating oil additives, fragrances, flavor materials or acrylates having a carbon-12 versus carbon-13 versus carbon-14 isotope ratio of about the same value as the CO₂ that occurs in the environment, wherein the biofuels, chemicals, polymers, surfactants, soaps, detergents, shampoos, lubricating oil additives, fragrances, flavor materials or acrylates are generated directly from or in combination with bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol or a bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway intermediate as disclosed herein.

Fatty alcohol, fatty aldehyde or fatty acid is a chemical used in commercial and industrial applications. Non-limiting examples of such applications include production of biofuels, chemicals, polymers, surfactants, soaps, detergents, shampoos, lubricating oil additives, fragrances, flavor materials and acrylates. Accordingly, in some embodiments, the invention provides biobased biofuels, chemicals, polymers, surfactants, soaps, detergents, shampoos, lubricating oil additives, fragrances, flavor materials and acrylates comprising one or more bioderived fatty alcohol, fatty aldehyde or fatty acid or bioderived fatty alcohol, fatty aldehyde or fatty acid pathway intermediate produced by a non-naturally occurring microorganism of the invention or produced using a method disclosed herein.

Isopropanol is a chemical used in commercial and industrial applications. Non-limiting examples of such applications include production of solvents, including rubbing alcohol. As a solvent, isopropanol is found in products such as paints, lacquers, thinners, inks, adhesives, general-purpose cleaners, disinfectants, cosmetics, toiletries, de-icers, and pharmaceuticals. Low-grade isoproapnol is also used in motor oils. Isopropanol is also used as a chemical intermediate for the production of isopropylamines, isopropylethers, and isopropyl esters. Isopropanol can potentially be dehydrated to form propylene, a polymer precursor. Accordingly, in some embodiments, the invention provides biobased solvents, rubbing alcohol, paints, lacquers, thinners, inks, adhesives, general-purpose cleaners, disinfectants, cosmetics, toiletries, de-icers, pharmaceuticals, motor oils, isopropylamines, isopropylethers, isopropyl esters, propylene and polymers, comprising bioderived isopropanol produced by a non-naturally occurring microorganism of the invention or produced using a method disclosed herein.

As used herein, the term “bioderived” means derived from or synthesized by a biological organism and can be considered a renewable resource since it can be generated by a biological organism. Such a biological organism, in particular the microbial organisms of the invention disclosed herein, can utilize feedstock or biomass, such as, sugars or carbohydrates obtained from an agricultural, plant, bacterial, or animal source. Alternatively, the biological organism can utilize atmospheric carbon. As used herein, the term “biobased” means a product as described above that is composed, in whole or in part, of a bioderived compound of the invention. A biobased or bioderived product is in contrast to a petroleum derived product, wherein such a product is derived from or synthesized from petroleum or a petrochemical feedstock.

In some embodiments, the invention provides a biofuel, chemical, polymer, surfactant, soap, detergent, shampoo, lubricating oil additive, fragrance, flavor material or acrylate comprising bioderived fatty alcohol, fatty aldehyde or fatty acid or bioderived fatty alcohol, fatty aldehyde or fatty acid pathway intermediate, wherein the bioderived fatty alcohol, fatty aldehyde or fatty acid or bioderived fatty alcohol, fatty aldehyde or fatty acid pathway intermediate includes all or part of the fatty alcohol, fatty aldehyde or fatty acid or fatty alcohol, fatty aldehyde or fatty acid pathway intermediate used in the production of a biofuel, chemical, polymer, surfactant, soap, detergent, shampoo, lubricating oil additive, fragrance, flavor material or acrylate. For example, the final biofuel, chemical, polymer, surfactant, soap, detergent, shampoo, lubricating oil additive, fragrance, flavor material or acrylate can contain the bioderived fatty alcohol, fatty aldehyde or fatty acid, fatty alcohol, fatty aldehyde or fatty acid pathway intermediate, or a portion thereof that is the result of the manufacturing of the biofuel, chemical, polymer, surfactant, soap, detergent, shampoo, lubricating oil additive, fragrance, flavor material or acrylate. Such manufacturing can include chemically reacting the bioderived fatty alcohol, fatty aldehyde or fatty acid, or bioderived fatty alcohol, fatty aldehyde or fatty acid pathway intermediate (e.g. chemical conversion, chemical functionalization, chemical coupling, oxidation, reduction, polymerization, copolymerization and the like) with itself or another compound in a reaction that produces the final biofuel, chemical, polymer, surfactant, soap, detergent, shampoo, lubricating oil additive, fragrance, flavor material or acrylate. Thus, in some aspects, the invention provides a biobased biofuel, chemical, polymer, surfactant, soap, detergent, shampoo, lubricating oil additive, fragrance, flavor material or acrylate comprising at least 2%, at least 3%, at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 98% or 100% bioderived fatty alcohol, fatty aldehyde or fatty acid or bioderived fatty alcohol, fatty aldehyde or fatty acid pathway intermediate as disclosed herein. In some aspects, when the product is a biobased polymer that includes or is obtained from a bioderived fatty alcohol, fatty aldehyde or fatty acid, or fatty alcohol, fatty aldehyde or fatty acid pathway intermediate described herein, the biobased polymer can be molded using methods well known in the art. Accordingly, in some embodiments, provided herein is a molded product comprising the biobased polymer described herein.

Additionally, in some embodiments, the invention provides a composition having a bioderived fatty alcohol, fatty aldehyde or fatty acid, or fatty alcohol, fatty aldehyde or fatty acid pathway intermediate disclosed herein and a compound other than the bioderived fatty alcohol, fatty aldehyde or fatty acid or fatty alcohol, fatty aldehyde or fatty acid pathway intermediate. For example, in some aspects, the invention provides a biobased biofuel, chemical, polymer, surfactant, soap, detergent, shampoo, lubricating oil additive, fragrance, flavor material or acrylate wherein the fatty alcohol, fatty aldehyde or fatty acid or fatty alcohol, fatty aldehyde or fatty acid pathway intermediate used in its production is a combination of bioderived and petroleum derived fatty alcohol, fatty aldehyde or fatty acid or fatty alcohol, fatty aldehyde or fatty acid pathway intermediate. For example, a biobased biofuel, chemical, polymer, surfactant, soap, detergent, shampoo, lubricating oil additive, fragrance, flavor material or acrylate can be produced using 50% bioderived fatty alcohol, fatty aldehyde or fatty acid and 50% petroleum derived fatty alcohol, fatty aldehyde or fatty acid or other desired ratios such as 60%/40%, 70%/30%, 80%/20%, 90%/10%, 95%/5%, 100%/0%, 40%/60%, 30%/70%, 20%/80%, 10%/90% of bioderived/petroleum derived precursors, so long as at least a portion of the product comprises a bioderived product produced by the microbial organisms disclosed herein. It is understood that methods for producing a biofuel, chemical, polymer, surfactant, soap, detergent, shampoo, lubricating oil additive, fragrance, flavor material or acrylate using the bioderived fatty alcohol, fatty aldehyde or fatty acid or bioderived fatty alcohol, fatty aldehyde or fatty acid pathway intermediate of the invention are well known in the art.

In some embodiments, the invention provides a solvent, a paint, lacquer, thinner, ink, adhesive, cleaner, disinfectant, cosmetic, toiletry, de-icer, pharmaceutical, motor oil, isopropylamine, isopropylether, isopropyl ester, propylene or a polymer comprising bioderived isopropanol or bioderived isopropanol pathway intermediate, wherein the bioderived isopropanol or bioderived isopropanol pathway intermediate includes all or part of the isopropanol or isopropanol pathway intermediate used in the production of a solvent, a paint, lacquer, thinner, ink, adhesive, cleaner, disinfectant, cosmetic, toiletry, de-icer, pharmaceutical, motor oil, isopropylamine, isopropylether, isopropyl ester, propylene or a polymer. For example, the final solvent, paint, lacquer, thinner, ink, adhesive, cleaner, disinfectant, cosmetic, toiletry, de-icer, pharmaceutical, motor oil, isopropylamine, isopropylether, isopropyl ester, propylene or polymer can contain the bioderived isopropanol, isopropanol pathway intermediate, or a portion thereof that is the result of the manufacturing of a solvent, paint, lacquer, thinner, ink, adhesive, cleaner, disinfectant, cosmetic, toiletry, de-icer, pharmaceutical, motor oil, isopropylamine, isopropylether, isopropyl ester, propylene or a polymer. Such manufacturing can include chemically reacting the bioderived isopropanol or bioderived isopropanol pathway intermediate (e.g. chemical conversion, chemical functionalization, chemical coupling, oxidation, reduction, polymerization, copolymerization and the like) into the final solvent, paint, lacquer, thinner, ink, adhesive, cleaner, disinfectant, cosmetic, toiletry, de-icer, pharmaceutical, motor oil, isopropylamine, isopropylether, isopropyl ester, propylene or polymer. Thus, in some aspects, the invention provides a biobased solvent, paint, lacquer, thinner, ink, adhesive, cleaner, disinfectant, cosmetic, toiletry, de-icer, pharmaceutical, motor oil, isopropylamine, isopropylether, isopropyl ester, propylene or polymer comprising at least 2%, at least 3%, at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 98% or 100% bioderived isopropanol or bioderived isopropanol pathway intermediate as disclosed herein.

Additionally, in some embodiments, the invention provides a composition having a bioderived isopropanol or isopropanol pathway intermediate disclosed herein and a compound other than the bioderived isopropanol or isopropanol pathway intermediate. For example, in some aspects, the invention provides a biobased solvent, paint, lacquer, thinner, ink, adhesive, cleaner, disinfectant, cosmetic, toiletry, de-icer, pharmaceutical, motor oil, isopropylamine, isopropylether, isopropyl ester, propylene or polymer wherein the isopropanol or isopropanol pathway intermediate used in its production is a combination of bioderived and petroleum derived isopropanol or isopropanol pathway intermediate. For example, a biobased solvent, paint, lacquer, thinner, ink, adhesive, cleaner, disinfectant, cosmetic, toiletry, de-icer, pharmaceutical, motor oil, isopropylamine, isopropylether, isopropyl ester, propylene or polymer can be produced using 50% bioderived isopropanol and 50% petroleum derived isopropanol or other desired ratios such as 60%/40%, 70%/30%, 80%/20%, 90%/10%, 95%/5%, 100%/0%, 40%/60%, 30%/70%, 20%/80%, 10%/90% of bioderived/petroleum derived precursors, so long as at least a portion of the product comprises a bioderived product produced by the microbial organisms disclosed herein. It is understood that methods for producing a solvent, paint, lacquer, thinner, ink, adhesive, cleaner, disinfectant, cosmetic, toiletry, de-icer, pharmaceutical, motor oil, isopropylamine, isopropylether, isopropyl ester, propylene or polymer using the bioderived isopropanol or bioderived isopropanol pathway intermediate of the invention are well known in the art.

The invention further provides a composition comprising bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol, and a compound other than the bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol. The compound other than the bioderived product can be a cellular portion, for example, a trace amount of a cellular portion of, or can be fermentation broth or culture medium, or a purified or partially purified fraction thereof produced in the presence of a non-naturally occurring microbial organism of the invention having a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway. The composition can comprise, for example, a reduced level of a byproduct when produced by an organism having reduced byproduct formation, as disclosed herein. The composition can comprise, for example, bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol, or a cell lysate or culture supernatant of a microbial organism of the invention.

In certain embodiments, provided herein is a composition comprising a bioderived fatty alcohol, fatty aldehyde or fatty acid provided herein, for example, a bioderived fatty alcohol, fatty aldehyde or fatty acid produced by culturing a non-naturally occurring microbial organism having a formaldehyde fixation pathway, a formate assimilation pathway and/or a methanol metabolic pathway, and a MI-FAE cycle, a MD-FAE cycle, and/or a FAACPE cycle in combination with a termination pathway, as provided herein. In some embodiments, the composition further comprises a compound other than said bioderived fatty alcohol, fatty aldehyde or fatty acid. In certain embodiments, the compound other than said bioderived fatty alcohol, fatty aldehyde or fatty acid is a trace amount of a cellular portion of a non-naturally occurring microbial organism having a formaldehyde fixation pathway, a formate assimilation pathway and/or a methanol metabolic pathway, and a MI-FAE cycle, a MD-FAE cycle, and/or a FAACPE cycle in combination with a termination pathway, as provided herein.

In certain embodiments, provided herein is a composition comprising bioderived isopropanol provided herein, for example, bioderived isopropanol produced by culturing a non-naturally occurring microbial organism having a formaldehyde fixation pathway, a formate assimilation pathway and/or a methanol metabolic pathway, and an isopropanol pathway, as provided herein. In some embodiments, the composition further comprises a compound other than said bioderived isopropanol. In certain embodiments, the compound other than said bioderived isopropanol is a trace amount of a cellular portion of a non-naturally occurring microbial organism having a formaldehyde fixation pathway, a formate assimilation pathway and/or a methanol metabolic pathway, and a isopropanol pathway, as provided herein.

In some embodiments, provided herein is a biobased product comprising a bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol provided herein. In certain embodiments, the biobased product is a biofuel, chemical, polymer, surfactant, soap, detergent, shampoo, lubricating oil additive, fragrance, flavor material or acrylate. In certain embodiments, the biobased product is a solvent, paint, lacquer, thinner, ink, adhesive, cleaner, disinfectant, cosmetic, toiletry, de-icer, pharmaceutical, motor oil, isopropylamine, isopropylether, isopropyl ester, propylene or polymer. In certain embodiments, the biobased product comprises at least 5% bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol. In certain embodiments, the biobased product comprises at least 10% bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol. In some embodiments, the biobased product comprises at least 20% bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol. In other embodiments, the biobased product comprises at least 30% bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol. In some embodiments, the biobased product comprises at least 40% bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol. In other embodiments, the biobased product comprises at least 50% bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol. In one embodiment, the biobased product comprises a portion of said bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol as a repeating unit. In another embodiment, provided herein is a molded product obtained by molding the biobased product provided herein. In other embodiments, provided herein is a process for producing a biobased product provided herein, comprising chemically reacting said bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol with itself or another compound in a reaction that produces said biobased product. In certain embodiments, provided herein is a polymer comprising or obtained by converting the bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol. In other embodiments, provided herein is a method for producing a polymer, comprising chemically of enzymatically converting the bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol to the polymer. In yet other embodiments, provided herein is a composition comprising the bioderived fatty alcohol, fatty aldehyde, fatty acid or isopropanol, or a cell lysate or culture supernatant thereof.

The culture conditions can include, for example, liquid culture procedures as well as fermentation and other large scale culture procedures. As described herein, particularly useful yields of the biosynthetic products of the invention can be obtained under anaerobic or substantially anaerobic culture conditions.

As described herein, one exemplary growth condition for achieving biosynthesis of fatty alcohol, fatty aldehyde, fatty acid or isopropanol includes anaerobic culture or fermentation conditions. In certain embodiments, the non-naturally occurring microbial organisms of the invention can be sustained, cultured or fermented under anaerobic or substantially anaerobic conditions. Briefly, an anaerobic condition refers to an environment devoid of oxygen. Substantially anaerobic conditions include, for example, a culture, batch fermentation or continuous fermentation such that the dissolved oxygen concentration in the medium remains between 0 and 10% of saturation. Substantially anaerobic conditions also includes growing or resting cells in liquid medium or on solid agar inside a sealed chamber maintained with an atmosphere of less than 1% oxygen. The percent of oxygen can be maintained by, for example, sparging the culture with an N₂/CO₂ mixture or other suitable non-oxygen gas or gases.

The culture conditions described herein can be scaled up and grown continuously for manufacturing of fatty alcohol, fatty aldehyde, fatty acid or isopropanol. Exemplary growth procedures include, for example, fed-batch fermentation and batch separation; fed-batch fermentation and continuous separation, or continuous fermentation and continuous separation. All of these processes are well known in the art. Fermentation procedures are particularly useful for the biosynthetic production of commercial quantities of fatty alcohol, fatty aldehyde, fatty acid or isopropanol. Generally, and as with non-continuous culture procedures, the continuous and/or near-continuous production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol will include culturing a non-naturally occurring fatty alcohol, fatty aldehyde, fatty acid or isopropanol producing organism of the invention in sufficient nutrients and medium to sustain and/or nearly sustain growth in an exponential phase. Continuous culture under such conditions can include, for example, growth or culturing for 1 day, 2, 3, 4, 5, 6 or 7 days or more. Additionally, continuous culture can include longer time periods of 1 week, 2, 3, 4 or 5 or more weeks and up to several months. Alternatively, organisms of the invention can be cultured for hours, if suitable for a particular application. It is to be understood that the continuous and/or near-continuous culture conditions also can include all time intervals in between these exemplary periods. It is further understood that the time of culturing the microbial organism of the invention is for a sufficient period of time to produce a sufficient amount of product for a desired purpose.

Fermentation procedures are well known in the art. Briefly, fermentation for the biosynthetic production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol can be utilized in, for example, fed-batch fermentation and batch separation; fed-batch fermentation and continuous separation, or continuous fermentation and continuous separation. Examples of batch and continuous fermentation procedures are well known in the art.

In addition to the above fermentation procedures using the fatty alcohol, fatty aldehyde, fatty acid or isopropanol producers of the invention for continuous production of substantial quantities of fatty alcohol, fatty aldehyde, fatty acid or isopropanol, the fatty alcohol, fatty aldehyde, fatty acid or isopropanol producers also can be, for example, simultaneously subjected to chemical synthesis and/or enzymatic procedures to convert the product to other compounds or the product can be separated from the fermentation culture and sequentially subjected to chemical and/or enzymatic conversion to convert the product to other compounds, if desired.

To generate better producers, metabolic modeling can be utilized to optimize growth conditions. Modeling can also be used to design gene knockouts that additionally optimize utilization of the pathway (see, for example, U.S. patent publications US 2002/0012939, US 2003/0224363, US 2004/0029149, US 2004/0072723, US 2003/0059792, US 2002/0168654 and US 2004/0009466, and U.S. Pat. No. 7,127,379). Modeling analysis allows reliable predictions of the effects on cell growth of shifting the metabolism towards more efficient production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol.

In addition to active and selective enzymes producing fatty alcohols, fatty aldehydes, fatty acid or isopropanols at high yield, titer and productivity, a robust host organism that can efficiently direct carbon and reducing equivalents to fatty alcohol, fatty aldehyde and fatty acid biosynthesis can be beneficial. Host modifications described herein are particularly useful in combination with selective enzymes described herein that favor formation of the desired fatty alcohol, fatty aldehyde, fatty acid or isopropanol product Several host modifications described herein entail introducing heterologous enzyme activities into the host organism. Other modifications involve overexpressing or elevating enzyme activity relative to wild type levels. Yet other modifications include disrupting endogenous genes or attenuating endogenous enzyme activities.

In one embodiment of the invention, the microbial organisms efficiently directs carbon and energy sources into production of acetyl-CoA, which is used as both a primer and extension unit in the MI-FAE cycle. In one embodiment of the invention, the microbial organisms efficiently directs carbon and energy sources into production of malonyl-CoA, which is used as both a primer and extension unit in the MD-FAE cycle. In unmodified microbial organism, fatty alcohol, fatty aldehyde and fatty acid production in the cytosol relies on the native cell machinery to provide the necessary precursors. Thus, high concentrations of cytosolic acetyl-CoA and/or malonyl-CoA are desirable for facilitating deployment of a cytosolic fatty alcohol, fatty aldehyde or fatty acid production pathway that originates from acetyl-CoA or malonyl-CoA. Metabolic engineering strategies for increasing cytosolic acetyl-CoA and malonyl-CoA are disclosed herein.

Since many eukaryotic organisms synthesize most of their acetyl-CoA in the mitochondria during growth on glucose, increasing the availability of acetyl-CoA in the cytosol can be obtained by introduction of a cytosolic acetyl-CoA biosynthesis pathway. Accordingly, acetyl-CoA biosynthesis pathways are described herein. In one embodiment, utilizing the pathways shown in FIG. 3, acetyl-CoA can be synthesized in the cytosol from a pyruvate or threonine precursor. In other embodiment, acetyl-CoA can be synthesized in the cytosol from phosphoenolpyruvate (PEP) or pyruvate (FIG. 4). In yet another embodiment acetyl-CoA can be synthesized in cellular compartments and transported to the cytosol. For example, one mechanism involves converting mitochondrial acetyl-CoA to a metabolic intermediate such as citrate or citramalate, transporting those intermediates to the cytosol, and then regenerating the acetyl-CoA (see FIGS. 5 and 6). Exemplary acetyl-CoA pathways and corresponding enzymes are further described in Examples V-VII.

In another embodiment, increasing cytosolic acetyl-CoA availability for fatty alcohol, fatty aldehyde, fatty acid or isopropanol biosynthesis is to disrupt or attenuate competing enzymes and pathways that utilize acetyl-CoA or its precursors. Exemplary competing enzyme activities include, but are not limited to, pyruvate decarboxylase, lactate dehydrogenase, short-chain aldehyde and alcohol dehydrogenases, acetate kinase, phosphotransacetylase, glyceraldehyde-3-phosphate dehydrogenases, pyruvate oxidase and acetyl-CoA carboxylase. Exemplary acetyl-CoA consuming pathways whose disruption or attenuation can improve fatty alcohol, fatty aldehyde, fatty acid or isopropanol production include the mitochondrial TCA cycle, fatty acid biosynthesis, ethanol production and amino acid biosynthesis. These enzymes and pathways are further described herein.

Yet another strategy for increasing cytosolic acetyl-CoA production is to increase the pool of CoA available in the cytoplasm. This can be accomplished by overexpression of CoA biosynthetic enzymes in the cytosol. In particular, expression of pantothenate kinase (EC 2.7.1.33) can be used. This enzyme catalyzes the first step and rate-limiting enzyme of CoA biosynthesis. Exemplary pantothenate kinase variants resistant to feedback inhibition by CoA are well known in the art (Rock et al, J Bacteriol 185: 3410-5 (2003)) and are described in the below table.

Protein Accession # GI number Organism coaA AAC76952 1790409 Escherichia coli CAB1 NP_010820.3 398366683 Saccharomyces cerevisiae KLLA0C00869g XP_452233.1 50304555 Kluyveromyces lactis YALI0D25476g XP_503275.1 50551601 Yarrowia lipolytica ANI_1_3272024 XP_001400486.2 317028058 Aspergillus niger

Competing enzymes and pathways that divert acyl-CoA substrates from production of fatty alcohols, fatty aldehydes or fatty acids of the invention can also be attenuated or disrupted. Exemplary enzymes for attenuation include acyltransferases, carnitine shuttle enzymes and negative regulators of MI-FAE cycle, MD-FAE cycle, FAACPE cycle or termination pathway enzymes.

Disruption or attenuation of acyltransferases that transfer acyl moieties from CoA to other acceptors such as ACP, glycerol, ethanol and others, can increase the availability of acyl-CoA for fatty alcohol, fatty aldehyde or fatty acid production. For example, Acyl-CoA:ACP transacylase (EC 2.3.1.38; 2.3.1.39) enzymes such as fabH (KASIII) of E. coli transfer acyl moieties from CoA to ACP. FabH is active on acetyl-CoA and butyryl-CoA (Prescott et al, Adv. Enzymol. Relat. Areas Mol, 36:269-311 (1972)). Acetyl-CoA:ACP transacylase enzymes from Plasmodium falciparum and Streptomyces avermitillis have been heterologously expressed in E. coli (Lobo et al, Biochem 40:11955-64 (2001)). A synthetic KASIII (FabH) from P. falciparum expressed in a fabH-deficient Lactococcus lactis host was able to complement the native fadH activity (Du et al, AEM 76:3959-66 (2010)). The acetyl-CoA:ACP transacylase enzyme from Spinacia oleracea accepts other acyl-ACP molecules as substrates, including butyryl-ACP (Shimakata et al, Methods Enzym 122:53-9 (1986)). Malonyl-CoA:ACP transacylase enzymes include FabD of E. coli and Brassica napsus (Verwoert et al, J Bacteriol, 174:2851-7 (1992); Simon et al, FEBS Lett 435:204-6 (1998)). FabD of B. napsus was able to complement fabD-deficient E. coli. The multifunctional eukaryotic fatty acid synthase enzyme complexes (described herein) also catalyze this activity. Other exemplary acyltransferases include diacylglycerol acyltransferases such as LRO1 and DGA1 of S. cerevisiae and DGA1 and DGA2 of Yarrowia lipolytica, glycerolipid acyltransferase enzymes such as plsB of E. coli (GenBank: AAC77011.2, GI:87082362; Heath and Rock, J Bacteriol 180:1425-30 (1998)), sterol acyltransferases such as ARE1 and ARE2 of S. cerevisiae, ethanol acyltransferases (EEB1, EHT1), putative acyltransferases (YMR210W) and others.

Protein GenBank ID GI Number Organism fabH AAC74175.1 1787333 Escherichia coli fadA NP_824032.1 29829398 Streptomyces avermitillis fabH AAC63960.1 3746429 Plasmodium falciparum Synthetic ACX34097.1 260178848 Plasmodium falciparum construct fabH CAL98359.1 124493385 Lactococcus lactis fabD AAC74176.1 1787334 Escherichia coli fabD CAB45522.1 5139348 Brassica napsus LRO1 NP_014405.1 6324335 Saccharomyces cerevisiae DGA1 NP_014888.1 6324819 Saccharomyces cerevisiae DGA1 CAG79269.1 49649549 Yarrowia lipolytica DGA2 XP_504700.1 50554583 Yarrowia lipolytica ARE1 NP_009978.1 6319896 Saccharomyces cerevisiae ARE2 NP_014416.1 6324346 Saccharomyces cerevisiae EEB1 NP_015230.1 6325162 Saccharomyces cerevisiae EHT1 NP_009736.3 398365307 Saccharomyces cerevisiae YMR210W NP_013937.1 6323866 Saccharomyces cerevisiae ALE1 NP_014818.1 6324749 Saccharomyces cerevisiae

Increasing production of fatty alcohols, fatty aldehydes or fatty acids may necessitate disruption or attenuation of enzymes involved in the trafficking of acetyl-CoA and acyl-CoA molecules from the cytosol to other compartments of the organism such as mitochondria, endoplasmic reticulum, proteoliposomes and peroxisomes. In these compartments, the acyl-CoA intermediate can be degraded or used as building blocks to synthesize fatty acids, cofactors and other byproducts.

Acetyl-CoA and acyl-CoA molecules localized in the cytosol can be transported into other cellular compartments with the aid of the carrier molecule carnitine via carnitine shuttles (van Roermund et al., EMBO J 14:3480-86 (1995)). Acyl-carnitine shuttles between cellular compartments have been characterized in yeasts such as Candida albicans (Strijbis et al, J Biol Chem 285:24335-46 (2010)). In these shuttles, the acyl moiety of acyl-CoA is reversibly transferred to carnitine by acylcarnitine transferase enzymes. Acetylcarnitine can then be transported across the membrane by organelle-specific acylcarnitine/carnitine translocase enzymes. After translocation, the acyl-CoA is regenerated by acetylcarnitine transferase Enzymes suitable for disruption or attenuation include carnitine acyltransferase enzymes, acylcarnitine translocases, acylcarnitine carrier proteins and enzymes involved in carnitine biosynthesis.

Carnitine acetyltransferase (CAT, EC 2.3.1.7) reversibly links acetyl units from acetyl-CoA to the carrier molecule, carnitine. Candida albicans encodes three CAT isozymes: Cat2, Yat1 and Yat2 (Strijbis et al., J Biol Chem 285:24335-46 (2010)). Cat2 is expressed in both the mitochondrion and the peroxisomes, whereas Yat1 and Yat2 are cytosolic. The Cat2 transcript contains two start codons that are regulated under different carbon source conditions. The longer transcript contains a mitochondrial targeting sequence whereas the shorter transcript is targeted to peroxisomes. Cat2 of Saccharomyces cerevisiae and AcuJ of Aspergillus nidulans employ similar mechanisms of dual localization (Elgersma et al., EMBO J 14:3472-9 (1995); Hynes et al., Euk Cell 10:547-55 (2011)). The cytosolic CAT of A. nidulans is encoded by facC. Other exemplary CAT enzymes are found in Rattus norvegicus and Homo sapiens (Cordente et al., Biochem 45:6133-41 (2006)). Exemplary carnitine acyltransferase enzymes (EC 2.3.1.21) are the Cpt1 and Cpt2 gene products of Rattus norvegicus (de Vries et al., Biochem 36:5285-92 (1997)).

Protein Accession # GI number Organism Cat2 AAN31660.1 23394954 Candida albicans Yat1 AAN31659.1 23394952 Candida albicans Yat2 XP_711005.1 68490355 Candida albicans Cat2 CAA88327.1 683665 Saccharomyces cerevisiae Yat1 AAC09495.1 456138 Saccharomyces cerevisiae Yat2 NP_010941.1 6320862 Saccharomyces cerevisiae AcuJ CBF69795.1 259479509 Aspergillus nidulans FacC AAC82487.1 2511761 Aspergillus nidulans Crat AAH83616.1 53733439 Rattus norvegicus Crat P43155.5 215274265 Homo sapiens Cpt1 AAB48046.1 1850590 Rattus norvegicus Cpt2 AAB02339.1 1374784 Rattus norvegicus

Carnitine-acylcarnitine translocases can catalyze the bidirectional transport of carnitine and carnitine-fatty acid complexes. The Cact gene product provides a mechanism for transporting acyl-carnitine substrates across the mitochondrial membrane (Ramsay et al Biochim Biophys Acta 1546:21-42 (2001)). A similar protein has been studied in humans (Sekoguchi et al., J Biol Chem 278:38796-38802 (2003)). The Saccharomyces cerevisiae mitochondrial carnitine carrier is Crc1 (van Roermund et al., supra; Palmieri et al., Biochimica et Biophys Acta 1757:1249-62 (2006)). The human carnitine translocase was able to complement a Crc1-deficient strain of S. cerevisiae (van Roermund et al., supra). Two additional carnitine translocases found in Drosophila melanogaster and Caenorhabditis elegans were also able to complement Crc1-deficient yeast (Oey et al., Mol Genet Metab 85:121-24 (2005)). Four mitochondrial carnitine/acetylcarnitine carriers were identified in Trypanosoma brucei based on sequence homology to the yeast and human transporters (Colasante et al., Mol Biochem Parasit 167:104-117 (2009)). The carnitine transporter of Candida albicans was also identified by sequence homology. An additional mitochondrial carnitine transporter is the acuH gene product of Aspergillus nidulans, which is exclusively localized to the mitochondrial membrane (Lucas et al., FEMS Microbiol Lett 201:193-8 (2006)).

Protein GenBank ID GI Number Organism Cact P97521.1 2497984 Rattus norvegicus Cacl NP_001034444.1 86198310 Homo sapiens CaO19.2851 XP_715782.1 68480576 Candida albicans Crc1 NP_014743.1 6324674 Saccharomyces cerevisiae Dif-1 CAA88283.1 829102 Caenorhabditis elegans colt CAA73099.1 1944534 Drosophila melanogaster Tb11.02.2960 EAN79492.1 70833990 Trypanosoma brucei Tb11.03.0870 EAN79007.1 70833505 Trypanosoma brucei Tb11.01.5040 EAN80288.1 70834786 Trypanosoma brucei Tb927.8.5810 AAX69329.1 62175181 Trypanosoma brucei acuH CAB44434.1 5019305 Aspergillus nidulans

Transport of camitine and acylcamitine across the peroxisomal membrane has not been well-characterized. Specific peroxisomal acylcamitine carrier proteins in yeasts have not been identified to date. However, mitochonidrial camitine translocases can also function in the peroxisomal transport of carnitine and acetylcarnifine. Experimental evidence suggests that the OCTN3 protein of Mus musculus is a peroxisomal camitine/acylcamitine translocase.

Yet another possibility is that acyl-CoA or acyl-camitine are transported across the peroxisomal or mitochondrial membranes by an acyl-CoA transporter such as the Pxa1 and Pxa2 ABC transporter of Saccharomyces cerevisiae or the ALDP ABC transporter of Homo sapiens (van Roermund et al., FASEB J 22:4201-8 (2008)). Pxa1 and Pxa2 (Pat1 and Pat2) form a heterodimeric complex in the peroxisomal membrane and catalyze the ATP-dependent transport of fatty acyl-CoA esters into the peroxisome (Verleur et al., Eur J Biochem 249: 657-61 (1997)). The mutant phenotype of a pxa1/pxa2 deficient yeast can be rescued by heterologous expression of ALDP, which was shown to transport a range of acyl-CoA substrates (van Roermund et al., FASEB J 22:4201-8 (2008)). Deletion of the Pxa12 transport system, in tandem with deletion of the peroxisomal fatty acyl-CoA synthetase (Faa2) abolished peroxisomal beta-oxidation in S. cerevisiae. Yet another strategy for reducing transport of pathway intermediates or products into the peroxisome is to attenuate or eliminate peroxisomal function, by interfering with systems involved in peroxisomal biogenesis. An exemplary target is Pex10 of Yarrowia lipolytica and homologs.

Protein Accession # GI number Organism OCTN3 BAA78343.1 4996131 Mus musculus Pxa1 AAC49009.1 619668 Saccharomyces cerevisiae Pxa2 AAB51597.1 1931633 Saccharomyces cerevisiae Faa2 NP_010931.3 398364331 Saccharomyces cerevisiae ALDP NP_000024.2 7262393 Homo sapiens Pex10 BAA99413.1 9049374 Yarrowia lipolytica

Carnitine biosynthetic pathway enzymes are also suitable candidates for disruption or attenuation. In Candida albicans, for example, camitine is synthesized from trimethyl-L-lysine in four enzymatic steps (Strijbis et al., FASEB J 23:2349-59 (2009)). The camitine pathway precursor, trimethyllysine (TML), is produced during protein degradation. TML dioxygenase (CaO13.4316) hydroxylates TML to form 3-hydroxy-6-N-trimethyllysine. A pyridoxal-5′-phoshpate dependent aldolase (CaO19.6305) then cleaves HTML into 4-trimethylaminobutyraldehyde. The 4-trimethylaminobutyraldehyde is subsequently oxidized to 4-trimethylaminobutyrate by a dehydrogenase (CaO19.6306). In the final step, 4-trimethylaminobutyrate is hydroxylated to form camitine by the gene product of CaO19.7131. Flux through the carnitine biosynthesis pathway is limited by the availability of the pathway substrate and very low levels of carnitine seem to be sufficient for normal carnitine shuttle activity (Strejbis et al., IUBMB Life 62:357-62 (2010)).

Protein Accession # GI number Organism CaO19.4316 XP_720623.1 68470755 Candida albicans CaO19.6305 XP_711090.1 68490151 Candida albicans CaO19.6306 XP_711091.1 68490153 Candida albicans CaO19.7131 XP_715182.1 68481628 Candida albicans

Carbon flux towards production of fatty alcohols, fatty aldehydes or fatty acids can be improved by deleting or attenuating competing pathways. Typical fermentation products of yeast include ethanol, glycerol and CO₂. The elimination or reduction of these byproducts can be accomplished by approaches described herein. For example, carbon loss due to respiration can be reduced. Other potential byproducts include lactate, acetate, formate, fatty acids and amino acids.

The conversion of acetyl-CoA into ethanol can be detrimental to the production of fatty alcohols, fatty aldehyes, fatty acids or isopropanol because the conversion process can draw away both carbon and reducing equivalents from the MI-FAE cycle, MD-FAE cycle, FAACPE cycle, termination pathway or isopropanol pathway. Ethanol can be formed from pyruvate in two enzymatic steps catalyzed by pyruvate decarboxylase and ethanol dehydrogenase. Saccharomyces cerevisiae has three pyruvate decarboxylases (PDC1, PDC5 and PDC6). PDC1 is the major isozyme and is strongly expressed in actively fermenting cells. PDC5 also functions during glycolytic fermentation, but is expressed only in the absence of PDC1 or under thiamine limiting conditions. PDC6 functions during growth on nonfermentable carbon sources. Deleting PDC1 and PDC5 can reduce ethanol production significantly; however these deletions can lead to mutants with increased PDC6 expression. Deletion of all three eliminates ethanol formation completely but also can cause a growth defect because of inability of the cells to form sufficient acetyl-CoA for biomass formation. This, however, can be overcome by evolving cells in the presence of reducing amounts of C2 carbon source (ethanol or acetate) (van Maris et al, AEM 69:2094-9 (2003)). It has also been reported that deletion of the positive regulator PDC2 of pyruvate decarboxylases PDC1 and PDC5, reduced ethanol formation to ˜10% of that made by wild-type (Hohmann et al, Mol Gen Genet 241:657-66 (1993)). Protein sequences and identifiers of PDC enzymes are listed in Example V.

Alternatively, alcohol dehydrogenases that convert acetaldehyde into ethanol and/or other short chain alcohol dehydrogenases can be disrupted or attenuated to provide carbon and reducing equivalents for the MI-FAE cycle, MD-FAE cycle, FAACPE cycle, termination pathway or isopropanol pathway. To date, seven alcohol dehydrogenases, ADHI-ADHVII, have been reported in S. cerevisiae (de Smidt et al, FEMS Yeast Res 8:967-78 (2008)). ADH1 (GI:1419926) is the key enzyme responsible for reducing acetaldehyde to ethanol in the cytosol under anaerobic conditions. It has been reported that a yeast strain deficient in ADH1 cannot grow anaerobically because an active respiratory chain is the only alternative path to regenerate NADH and lead to a net gain of ATP (Drewke et al, J Bacteriol 172:3909-17 (1990)). This enzyme is an ideal candidate for downregulation to limit ethanol production. ADH2 is severely repressed in the presence of glucose. In K. lactis, two NAD-dependent cytosolic alcohol dehydrogenases have been identified and characterized. These genes also show activity for other aliphatic alcohols. The genes ADH1 (GI:113358) and ADHII (GI:51704293) are preferentially expressed in glucose-grown cells (Bozzi et al, Biochim Biophys Acta 1339:133-142 (1997)). Cytosolic alcohol dehydrogenases are encoded by ADH1 (GI:608690) in C. albicans, ADH1 (GI:3810864) in S. pombe, ADH1 (GI:5802617) in Y. lipolytica, ADH1 (GI:2114038) and ADHII (GI:2143328) in Pichia stipitis or Scheffersomyces stipitis (Passoth et al, Yeast 14:1311-23 (1998)). Candidate alcohol dehydrogenases are shown the table below.

Protein GenBank ID GI number Organism SADH BAA24528.1 2815409 Candida parapsilosis ADH1 NP_014555.1 6324486 Saccharomyces cerevisiae s288c ADH2 NP_014032.1 6323961 Saccharomyces cerevisiae s288c ADH3 NP_013800.1 6323729 Saccharomyces cerevisiae s288c ADH4 NP_011258.2 269970305 Saccharomyces cerevisiae s288c ADH5 (SFA1) NP_010113.1 6320033 Saccharomyces cerevisiae s288c ADH6 NP_014051.1 6323980 Saccharomyces cerevisiae s288c ADH7 NP_010030.1 6319949 Saccharomyces cerevisiae s288c adhP CAA44614.1 2810 Kluyveromyces lactis ADH1 P20369.1 113358 Kluyveromyces lactis ADH2 CAA45739.1 2833 Kluyveromyces lactis ADH3 P49384.2 51704294 Kluyveromyces lactis ADH1 CAA57342.1 608690 Candida albicans ADH2 CAA21988.1 3859714 Candida albicans SAD XP_712899.1 68486457 Candida albicans ADH1 CAA21782.1 3810864 Schizosaccharomyces pombe ADH1 AAD51737.1 5802617 Yarrowia lipolytica ADH2 AAD51738.1 5802619 Yarrowia lipolytica ADH3 AAD51739.1 5802621 Yarrowia lipolytica AlcB AAX53105.1 61696864 Aspergillus niger ANI_1_282024 XP_001399347.1 145231748 Aspergillus niger ANI_1_126164 XP_001398574.2 317037131 Aspergillus niger ANI_1_1756104 XP_001395505.2 317033815 Aspergillus niger ADH2 CAA73827.1 2143328 Scheffersomyces stipitis

Attenuation or disruption of one or more glycerol-3-phosphatase or glycerol-3-phosphate (G3P) dehydrogenase enzymes can eliminate or reduce the formation of glycerol, and thereby conserving carbon and reducing equivalents for production of fatty alcohols, fatty aldehydes, fatty acids or isopropnaol.

G3P phosphatase catalyzes the hydrolysis of G3P to glycerol Enzymes with this activity include the glycerol-1-phosphatase (EC 3.1.3.21) enzymes of Saccharomyces cerevisiae (GPP1 and GPP2), Candida albicans and Dunaleilla parva (Popp et al, Biotechnol Bioeng 100:497-505 (2008); Fan et al, FEMS Microbiol Lett 245:107-16 (2005)). The D. parva gene has not been identified to date. These and additional G3P phosphatase enzymes are shown in the table below.

Protein GenBank ID GI Number Organism GPP1 DAA08494.1 285812595 Saccharomyces cerevisiae GPP2 NP_010984.1 6320905 Saccharomyces cerevisiae GPP1 XP_717809.1 68476319 Candida albicans KLLA0C08217g XP_452565.1 50305213 Kluyveromyces lactis KLLA0C11143g XP_452697.1 50305475 Kluyveromyces lactis ANI_1_380074 XP_001392369.1 145239445 Aspergillus niger ANI_1_444054 XP_001390913.2 317029125 Aspergillus niger

S. cerevisiae has three G3P dehydrogenase enzymes encoded by GPD1 and GDP2 in the cytosol and GUT2 in the mitochondrion. GPD2 is known to encode the enzyme responsible for the majority of the glycerol formation and is responsible for maintaining the redox balance under anaerobic conditions. GPD1 is primarily responsible for adaptation of S. cerevisiae to osmotic stress (Bakker et al., FEMS Microbiol Rev 24:15-37 (2001)). Attenuation of GPD1, GPD2 and/or GUT2 will reduce glycerol formation. GPD1 and GUT2 encode G3P dehydrogenases in Yarrowia lipolytica (Beopoulos et al, AEM 74:7779-89 (2008)). GPD1 and GPD2 encode for G3P dehydrogenases in S. pombe. Similarly, G3P dehydrogenase is encoded by CTRG_02011 in Candida tropicalis and a gene represented by GI:20522022 in Candida albicans.

Protein GenBank ID GI number Organism GPD1 CAA98582.1 1430995 Saccharomyces cerevisiae GPD2 NP_014582.1 6324513 Saccharomyces cerevisiae GUT2 NP_012111.1 6322036 Saccharomyces cerevisiae GPD1 CAA22119.1 6066826 Yarrowia lipolytica GUT2 CAG83113.1 49646728 Yarrowia lipolytica GPD1 CAA22119.1 3873542 Schizosaccharomyces pombe GPD2 CAA91239.1 1039342 Schizosaccharomyces pombe ANI_1_786014 XP_001389035.2 317025419 Aspergillus niger ANI_1_1768134 XP_001397265.1 145251503 Aspergillus niger KLLA0C04004g XP_452375.1 50304839 Kluyveromyces lactis CTRG_02011 XP_002547704.1 255725550 Candida tropicalis GPD1 XP_714362.1 68483412 Candida albicans GPD2 XP_713824.1 68484586 Candida albicans

Enzymes that form acid byproducts such as acetate, formate and lactate can also be attenuated or disrupted. Such enzymes include acetate kinase, phosphotransacetylase and pyruvate oxidase. Disruption or attenuation of pyruvate formate lyase and formate dehydrogenase could limit formation of formate and carbon dioxide. These enzymes are described in further detail in Example V.

Alcohol dehydrogenases that convert pyruvate to lactate are also candidates for disruption or attenuation. Lactate dehydrogenase enzymes include ldhA of E. coli and ldh from Ralstonia eutropha (Steinbuchel and Schlegel, Eur. J. Biochem. 130:329-334 (1983)). Other alcohol dehydrogenases listed above may also exhibit LDH activity.

Protein GenBank ID GI number Organism ldhA NP_415898.1 16129341 Escherichia coli Ldh YP_725182.1 113866693 Ralstonia eutropha

Tuning down activity of the mitochondrial pyruvate dehydrogenase complex will limit flux into the mitochondrial TCA cycle. Under anaerobic conditions and in conditions where glucose concentrations are high in the medium, the capacity of this mitochondrial enzyme is very limited and there is no significant flux through it. However, in some embodiments, this enzyme can be disrupted or attenuated to increase fatty alcohol, fatty aldehyde or fatty acid production. Exemplary pyruvate dehydrogenase genes include PDB1, PDA1, LAT1 and LPD1. Accession numbers and homologs are listed in Example V.

Another strategy for reducing flux into the TCA cycle is to limit transport of pyruvate into the mitochondria by tuning down or deleting the mitochondrial pyruvate carrier. Transport of pyruvate into the mitochondria in S. cerevisiae is catalyzed by a heterocomplex encoded by MPC1 and MPC2 (Herzig et al, Science 337:93-6 (2012); Bricker et al, Science 337:96-100 (2012)). S. cerevisiae encodes five other putative monocarboxylate transporters (MCH1-5), several of which may be localized to the mitochondrial membrane (Makuc et al, Yeast 18:1131-43 (2001)). NDT1 is another putative pyruvate transporter, although the role of this protein is disputed in the literature (Todisco et al, J Biol Chem 20:1524-31 (2006)). Exemplary pyruvate and monocarboxylate transporters are shown in the table below:

Protein GenBank ID GI number Organism MPC1 NP_011435.1 6321358 Saccharomyces cerevisiae MPC2 NP_012032.1 6321956 Saccharomyces cerevisiae MPC1 XP_504811.1 50554805 Yarrowia lipolytica MPC2 XP_501390.1 50547841 Yarrowia lipolytica MPC1 XP_719951.1 68471816 Candida albicans MPC2 XP_716190.1 68479656 Candida albicans MCH1 NP_010229.1 6320149 Saccharomyces cerevisiae MCH2 NP_012701.2 330443640 Saccharomyces cerevisiae MCH3 NP_014274.1 6324204 Saccharomyces cerevisiae MCH5 NP_014951.2 330443742 Saccharomyces cerevisiae NDT1 NP_012260.1 6322185 Saccharomyces cerevisiae ANI_1_1592184 XP_001401484.2 317038471 Aspergillus niger CaJ7_0216 XP_888808.1 77022728 Candida albicans YALI0E16478g XP_504023.1 50553226 Yarrowia lipolytica KLLA0D14036g XP_453688.1 50307419 Kluyveromyces lactis

Disruption or attenuation of enzymes that synthesize malonyl-CoA and fatty acids can increase the supply of carbon available for fatty alcohol, fatty aldehyde or fatty acid biosynthesis from acetyl-CoA. Exemplary enzymes for disruption or attenuation include fatty acid synthase, acetyl-CoA carboxylase, biotin:apoenzyme ligase, acyl carrier protein, thioesterase, acyltransferases, ACP malonyltransferase, fatty acid elongase, acyl-CoA synthetase, acyl-CoA transferase and acyl-CoA hydrolase.

Another strategy to reduce fatty acid biosynthesis is expression or overexpression of regulatory proteins which repress fatty acid forming genes. Acetyl-CoA carboxylase (EC 6.4.1.2) catalyzes the first step of fatty acid biosynthesis in many organisms: the ATP-dependent carboxylation of acetyl-CoA to malonyl-CoA. This enzyme utilizes biotin as a cofactor. Exemplary ACC enzymes are encoded by accABCD of E. coli (Davis et al, J Biol Chem 275:28593-8 (2000)), ACC1 of Saccharomyces cerevisiae and homologs (Sumper et al, Methods Enzym 71:34-7 (1981)). The mitochondrial acetyl-CoA carboxylase of S. cerevisiae is encoded by HFA1. Acetyl-CoA carboxylase holoenzyme formation requires attachment of biotin by a biotin:apoprotein ligase such as BPL1 of S. cerevisiae.

Protein GenBank ID GI Number Organism ACC1 CAA96294.1 1302498 Saccharomyces cerevisiae KLLA0F06072g XP_455355.1 50310667 Kluyveromyces lactis ACC1 XP_718624.1 68474502 Candida albicans YALI0C11407p XP_501721.1 50548503 Yarrowia lipolytica ANI_1_1724104 XP_001395476.1 145246454 Aspergillus niger accA AAC73296.1 1786382 Escherichia coli accB AAC76287.1 1789653 Escherichia coli accC AAC76288.1 1789654 Escherichia coli accD AAC75376.1 1788655 Escherichia coli HFA1 NP_013934.1 6323863 Saccharomyces cerevisiae BPL1 NP_010140.1 6320060 Saccharomyces cerevisiae

Proteins participating in the synthesis of fatty acids are shown below. The fatty acid synthase enzyme complex of yeast is composed of two multifunctional subunits, FAS1 and FAS2, which together catalyze the net conversion of acetyl-CoA and malonyl-CoA to fatty acids (Lomakin et al, Cell 129: 319-32 (2007)). Additional proteins associated with mitochondrial fatty acid synthesis include OAR1, Mct1, E1R1, ACP1 and PPT2. ACP1 is the mitochondrial acyl carrier protein and PPT2 encodes a phosphopantetheine transferase, which pantetheinylates mitochondrial ACP and is required for fatty acid biosynthesis in the mitochondria (Stuible et al, J Biol Chem: 273: 22334-9 (1998)). A non-genetic strategy for reducing activity of fatty acid synthases is to add an inhibitor such as cerulenin. Global regulators of lipid biosynthesis can also be altered to tune down endogenous fatty acid biosynthesis pathways during production of long chain alcohols or related products. An exemplary global regulator is SNF1 of Yarrowia lipolytica and Saccharomyces cerevisiae.

Protein GenBank ID GI Number Organism FAS1 NP_012739.1 6322666 Saccharomyces cerevisiae FAS2 NP_015093.1 6325025 Saccharomyces cerevisiae FAS1 XP_451653.1 50303423 Kluyveromyces lactis FAS2 XP_452914.1 50305907 Kluyveromyces lactis FAS1 XP_716817.1 68478392 Candida albicans FAS2 XP_723014.1 68465892 Candida albicans FAS1 XP_500912.1 50546885 Yarrowia lipolytica FAS2 XP_501096.1 50547253 Yarrowia lipolytica FAS1 XP_001393490.2 317031809 Aspergillus niger FAS2 XP_001388458.1 145228299 Aspergillus niger OAR1 NP_012868.1 6322795 Saccharomyces cerevisiae MCT1 NP_014864.4 398365823 Saccharomyces cerevisiae ETR1 NP_009582.1 6319500 Saccharomyces cerevisiae ACP1 NP_012729.1 6322656 Saccharomyces cerevisiae PPT2 NP_015177.2 37362701 Saccharomyces cerevisiae SNF1 CAG80498.1 49648180 Yarrowia lipolytica SNF1 P06782.1 134588 Saccharomyces cerevisiae

Disruption or attenuation of elongase enzymes which convert acyl-CoA substrates to longer-chain length fatty acid derivatives longer than the product of interest can also be used to increase fatty alcohol, fatty aldehyde or fatty acid production. Elongase enzymes are found in compartments such as the mitochondria, endoplasmic reticulum, proteoliposomes and peroxisomes. For example, some yeast such as S. cerevisiae are able to synthesize long-chain fatty acids of chain length C16 and higher via a mitochondrial elongase which accepts exogenous or endogenous acyl-CoA substrates (Bessoule et al, FEBS Lett 214: 158-162 (1987)). This system requires ATP for activity. The endoplasmic reticulum also has an elongase system for synthesizing very long chain fatty acids (C18+) from acyl-CoA substrates of varying lengths (Kohlwein et al, Mol Cell Biol 21:109-25 (2001)). Genes involved in this system include TSC13, ELO2 and ELO3. ELO1 catalyzes the elongation of C12 acyl-CoAs to C16-C18 fatty acids.

Protein Accession # GI number Organism ELO2 NP_009963.1 6319882 Saccharomyces cerevisiae ELO3 NP_013476.3 398366027 Saccharomyces cerevisiae TSC13 NP_010269.1 6320189 Saccharomyces cerevisiae ELO1 NP_012339.1 6322265 Saccharomyces cerevisiae

Native enzymes converting acyl-CoA pathway intermediates to acid byproducts can also reduce fatty alcohol, fatty aldehyde or fatty acid yield. For example, CoA hydrolases, transferases and synthetases can act on acyl-CoA intermediates to form short-, medium- or long chain acids. Disruption or attenuation of endogenous CoA hydrolases, CoA transerases and/or reversible CoA synthetases can be used to increase fatty alcohol, fatty aldehyde or fatty acid yield. Exemplary enzymes are shown in the table below.

Protein GenBank ID GI number Organism Tes1 NP_012553.1 6322480 Saccharomyces cerevisiae s288c ACH1 NP_009538.1 6319456 Saccharomyces cerevisiae s288c EHD3 NP_010321.1 6320241 Saccharomyces cerevisiae s288c YALI0F14729p XP_505426.1 50556036 Yarrowia lipolytica YALI0E30965p XP_504613.1 50554409 Yarrowia lipolytica KLLA0E16523g XP_454694.1 50309373 Kluyveromyces lactis KLLA0E10561g XP_454427.1 50308845 Kluyveromyces lactis ACH1 P83773.2 229462795 Candida albicans CaO19.10681 XP_714720.1 68482646 Candida albicans ANI_1_318184 XP_001401512.1 145256774 Aspergillus niger ANI_1_1594124 XP_001401252.2 317035188 Aspergillus niger tesB NP_414986.1 16128437 Escherichia coli tesB NP_355686.2 159185364 Agrobacterium tumefaciens atoA 2492994 P76459.1 Escherichia coli atoD 2492990 P76458.1 Escherichia coli

Enzymes that favor the degradation of products, MI-FAE cycle intermediates, MD-FAE cycle intermediates, FAACPE cycle intermediates, termination pathway intermediates, or isopropanol pathway intermediates can also be disrupted or attenuated. Examples include aldehyde dehydrogenases, aldehyde decarbonylases, oxidative alcohol dehydrogenases, and irreversible fatty acyl-CoA degrading enzymes.

For production of fatty alcohols, fatty aldehydes, fatty acids or isopropanol of the invention, deletion or attenuation of non-specific aldehyde dehydrogenases can improve yield. For production of fatty acids, expression of such an enzyme may improve product formation. Such enzymes can, for example, convert acetyl-CoA into acetaldehyde, fatty aldehydes to fatty acids, or fatty alcohols to fatty acids. Acylating aldehyde dehydrogenase enzymes are described in Example IV. Acid-forming aldehyde dehydrogenase are described in Examples VI and XII.

The pathway enzymes that favor the reverse direction can also be disrupted or attenuated, if they are detrimental to fatty alcohol, fatty aldehyde, fatty acid or isopropanol production. An example is long chain alcohol dehydrogenases (EC 1.1.1.192) that favor the oxidative direction. Exemplary long chain alcohol dehydrogenases are ADH1 and ADH2 of Geobacillus thermodenitrificans, which oxidize alcohols up to a chain length of C30 (Liu et al, Physiol Biochem 155:2078-85 (2009)). These and other exemplary fatty alcohol dehydrogenase enzymes are listed in Examples IV and V. If an alcohol-forming acyl-CoA reductase is utilized for fatty alcohol, fatty aldehyde or fatty acid biosynthesis, deletion of endogenous fatty alcohol dehydrogenases will substantially reduce backflux.

Beta-oxidation enzymes may be reversible and operate in the direction of acyl-CoA synthesis. However, if they are irreversible or strongly favored in the degradation direction they are candidates for disruption or attenuation. An enzyme that fall into this category includes FOX2 of S. cerevisiae, a multifunctional enzyme with 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activity (Hiltunen et al, J Biol Chem 267: 6646-6653 (1992)). Additional genes include degradative thiolases such as POT1 and acyl-CoA dehydrogenases that utilize cofactors other than NAD(P)H (EG. EC 1.3.8.-) such as fadE of E. coli.

Protein GenBank ID GI Number Organism POT1 NP_012106.1 6322031 Saccharomyces cerevisiae FOX2 NP_012934.1 6322861 Saccharomyces cerevisiae fadE AAC73325.2 87081702 Escherichia coli

Fatty acyl-CoA oxidase enzymes such as PDX1 of S. cerevisiae catalyze the oxygen-dependent oxidation of fatty acyl-CoA substrates Enzymes with this activity can be disrupted or attenuated, if they are expressed under fatty alcohol, fatty aldehyde or fatty acid producing conditions. POX1 (EC 1.3.3.6) genes and homologs are shown in the table below. POX1 is subject to regulation by OAF1, which also activates genes involved in peroxisomal beta-oxidation, organization and biogenesis (Luo et al, J Biol Chem 271:12068-75 (1996)). Regulators with functions similar to OAF1, and peroxisomal fatty acid transporters PXA1 and PXA2 are also candidates for deletion.

Protein GenBank ID GI Number Organism POX1 NP_011310.1 6321233 Saccharomyces cerevisiae OAF1 NP_009349.3 330443370 Saccharomyces cerevisiae PXA1 NP_015178.1 6325110 Saccharomyces cerevisiae PXA2 NP_012733.1 6322660 Saccharomyces cerevisiae YALI0F10857g XP_505264.1 50555712 Yarrowia lipolytica YALI0D24750p XP_503244.1 50551539 Yarrowia lipolytica YALI0E32835p XP_504703.1 50554589 Yarrowia lipolytica YALI0E06567p XP_503632.1 50552444 Yarrowia lipolytica YALI0E27654p XP_504475.1 50554133 Yarrowia lipolytica YALI0C23859p XP_502199.1 50549457 Yarrowia lipolytica POX XP_455532.1 50311017 Kluyveromyces lactis POX104 XP_721610.1 68468582 Candida albicans POX105 XP_717995.1 68475844 Candida albicans POX102 XP_721613.1 68468588 Candida albicans

Another candidate for disruption or attenuation is an acyl-CoA binding protein. The acyl binding protein ACB1 of S. cerevisiae, for example, binds acyl-CoA esters and shuttles them to acyl-CoA utilizing processes (Schjerling et al, J Biol Chem 271: 22514-21 (1996)). Deletion of this protein did not impact growth rate and lead to increased accumulation of longer-chain acyl-CoA molecules. Acyl-CoA esters are involved in diverse cellular processes including lipid biosynthesis and homeostatis, signal transduction, growth regulation and cell differentiation (Rose et al, PNAS USA 89: 11287-11291 (1992)).

Protein GenBank ID GI Number Organism ACB1 P31787.3 398991 Saccharomyces cerevisiae KLLA0B05643g XP_451787.2 302309983 Kluyveromyces lactis YALI0E23185g XP_002143080.1 210076210 Yarrowia lipolytica ANI_1_1084034 XP_001390082.1 145234867 Aspergillus niger

To achieve high yields of fatty alcohols, fatty aldehydes fatty acids or isopropanol, it is desirable that the host organism can supply the cofactors required by the MI-FAE cycle, MD-FAE cycle, FAACPE cycle, the termination pathway and/or isopropanol pathway in sufficient quantities. In several organisms, in particular eukaryotic organisms, such as several Saccharomyces, Kluyveromyces, Candida, Aspergillus, and Yarrowia species, NADH is more abundant than NADPH in the cytosol as it is produced in large quantities by glycolysis. NADH can be made even more abundant by converting pyruvate to acetyl-CoA by means of heterologous or native NAD-dependant enzymes such as NAD-dependant pyruvate dehydrogenase, NAD-dependant formate dehydrogenase, NADH:ferredoxin oxidoreductase, or NAD-dependant acylating acetylaldehyde dehydrogenase in the cytosol. Given the abundance of NADH in the cytosol of most organisms, it can be beneficial for all reduction steps of the MI-FAE cycle, MD-FAE cycle, FAACPE cycle, termination pathway and/or isopropanol pathway to accept NADH as the reducing agent preferentially over other reducing agents such as NADPH. High yields of fatty alcohols, fatty aldehydes or fatty acids can thus be accomplished by, for example: 1) identifying and implementing endogenous or exogenous MI-FAE cycle, MD-FAE cycle and/or termination pathway enzymes with a stronger preference for NADH than other reducing equivalents such as NADPH; 2) attenuating one or more endogenous MI-FAE cycle, MD-FAE cycle or termination pathway enzymes that contribute NADPH-dependant reduction activity; 3) altering the cofactor specificity of endogenous or exogenous MI-FAE cycle, MD-FAE cycle, FAACPE cycle, termination pathway or isopropanol pathway enzymes so that they have a stronger preference for NADH than their natural versions; or 4) altering the cofactor specificity of endogenous or exogenous MI-FAE cycle, MD-FAE cycle, FAACPE cycle, termination pathway or isopropanol pathway enzymes so that they have a weaker preference for NADPH than their natural versions.

Strategies for engineering NADH-favoring MI-FAE cycle, MD-FAE cycle, FAACPE cycle termination pathways and/or isopropanol pathways are described in further detail in Example VIII. Methods for changing the cofactor specificity of an enzyme are well known in the art, and an example is described in Example IX.

If one or more of the MI-FAE cycle, MD-FAE cycle, FAACPE cycle, termination pathway and/or isopropanol pathway enzymes utilizes NADPH as the cofactor, it can be beneficial to increase the production of NADPH in the host organism. In particular, if the MI-FAE cycle, MD-FAE cycle, FAACPE cycle, termination pathway and/or isopropanol pathway is present in the cytosol of the host organism, methods for increasing NADPH production in the cytosol can be beneficial. Several approaches for increasing cytosolic production of NADPH can be implemented including channeling an increased amount of flux through the oxidative branch of the pentose phosphate pathway relative to wild-type, channeling an increased amount of flux through the Entner Doudoroff pathway relative to wild-type, introducing a soluble or membrane-bound transhydrogenase to convert NADH to NADPH, or employing NADP-dependant versions of the following enzymes: phosphorylating or non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase, pyruvate dehydrogenase, formate dehydrogenase, or acylating acetylaldehyde dehydrogenase. These activities can be augmented by disrupting or attenuating native NAD-dependant enzymes including glyceraldehyde-3-phosphate dehydrogenase, pyruvate dehydrogenase, formate dehydrogenase, or acylating acetylaldehyde dehydrogenase. Strategies for engineering increased NADPH availability are described in Example X.

Synthesis of fatty alcohols, fatty aldehyes, fatty acids or isopropanol in the cytosol can be dependent upon the availability of sufficient carbon and reducing equivalents. Therefore, without being bound to any particular theory of operation, increasing the redox ratio of NAD(P)H to NAD(P) can help drive the MI-FAE cycle, MD-FAE cycle, FAACPE cycle, termination pathway and/or isopropanol pathway in the forward direction. Methods for increasing the redox ratio of NAD(P)H to NAD(P) include limiting respiration, attenuating or disrupting competing pathways that produce reduced byproducts such as ethanol and glycerol, attenuating or eliminating the use of NADH by NADH dehydrogenases, and attenuating or eliminating redox shuttles between compartments.

One exemplary method to provide an increased number of reducing equivalents, such as NAD(P)H, for enabling the formation of fatty alcohols, fatty aldehydes, fatty acids or isopropanol is to constrain the use of such reducing equivalents during respiration. Respiration can be limited by: reducing the availability of oxygen, attenuating NADH dehydrogenases and/or cytochrome oxidase activity, attenuating G3P dehydrogenase, and/or providing excess glucose to Crabtree positive organisms.

Restricting oxygen availability by culturing the non-naturally occurring eukaryotic organisms in a fermenter is one example for limiting respiration and thereby increasing the ratio of NAD(P)H to NAD(P). The ratio of NAD(P)H/NAD(P) increases as culture conditions become more anaerobic, with completely anaerobic conditions providing the highest ratios of the reduced cofactors to the oxidized ones. For example, it has been reported that the ratio of NADH/NAD=0.02 in aerobic conditions and 0.75 in anaerobic conditions in E. coli (de Graes et al, J Bacteriol 181:2351-57 (1999)).

Respiration can also be limited by reducing expression or activity of NADH dehydrogenases and/or cytochrome oxidases in the cell under aerobic conditions. In this case, respiration can be limited by the capacity of the electron transport chain. Such an approach has been used to enable anaerobic metabolism of E. coli under completely aerobic conditions (Portnoy et al, AEM 74:7561-9 (2008)). S. cerevisiae can oxidize cytosolic NADH directly using external NADH dehydrogenases, encoded by NDE1 and NDE2. One such NADH dehydrogenase in Yarrowia lipolytica is encoded by NDH2 (Kerscher et al, J Cell Sci 112:2347-54 (1999)). These and other NADH dehydrogenase enzymes are listed in the table below.

Protein GenBank ID GI number Organism NDE1 NP_013865.1 6323794 Saccharomyces cerevisiae s288c NDE2 NP_010198.1 6320118 Saccharomyces cerevisiae s288c NDH2 AJ006852.1 3718004 Yarrowia lipolytica ANI_1_610074 XP_001392541.2 317030427 Aspergillus niger ANI_1_2462094 XP_001394893.2 317033119 Aspergillus niger KLLA0E21891g XP_454942.1 50309857 Kluyveromyces lactis KLLA0C06336g XP_452480.1 50305045 Kluyveromyces lactis NDE1 XP_720034.1 68471982 Candida albicans NDE2 XP_717986.1 68475826 Candida albicans

Cytochrome oxidases of Saccharomyces cerevisiae include the COX gene products. COX1-3 are the three core subunits encoded by the mitochondrial genome, whereas COX4-13 are encoded by nuclear genes. Attenuation or disruption of any of the cytochrome genes results in a decrease or block in respiratory growth (Heimann and Funes, Gene 354:43-52 (2005)). Cytochrome oxidase genes in other organisms can be inferred by sequence homology.

Protein GenBank ID GI number Organism COX1 CAA09824.1 4160366 Saccharomyces cerevisiae s288c COX2 CAA09845.1 4160387 Saccharomyces cerevisiae s288c COX3 CAA09846.1 4160389 Saccharomyces cerevisiae s288c COX4 NP_011328.1 6321251 Saccharomyces cerevisiae s288c COX5A NP_014346.1 6324276 Saccharomyces cerevisiae s288c COX5B NP_012155.1 6322080 Saccharomyce cerevisiae s288c COX6 NP_011918.1 6321842 Saccharomyces cerevisiae s288c COX7 NP_013983.1 6323912 Saccharomyces cerevisiae s288c COX8 NP_013499.1 6323427 Saccharomyces cerevisiae s288c COX9 NP_010216.1 6320136 Saccharomyces cerevisiae s288c COX12 NP_013139.1 6323067 Saccharomyces cerevisiae s288c COX13 NP_011324.1 6321247 Saccharomyces cerevisiae s288c

Cytosolic NADH can also be oxidized by the respiratory chain via the G3P dehydrogenase shuttle, consisting of cytosolic NADH-linked G3P dehydrogenase and a membrane-bound G3P:ubiquinone oxidoreductase. The deletion or attenuation of G3P dehydrogenase enzymes will also prevent the oxidation of NADH for respiration. Enzyme candidates encoding these enzymes are described herein.

Additionally, in Crabtree positive organisms, fermentative metabolism can be achieved in the presence of excess of glucose. For example, S. cerevisiae makes ethanol even under aerobic conditions. The formation of ethanol and glycerol can be reduced/eliminated and replaced by the production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol in a Crabtree positive organism by feeding excess glucose to the Crabtree positive organism. In another aspect, provided herein is a method for producing fatty alcohols, fatty aldehydes, fatty acids, isopropanol comprising culturing a non-naturally occurring eukaryotic organism under conditions and for a sufficient period of time to produce fatty alcohol, fatty aldehyde, fatty acid or isopropanol, wherein the eukaryotic organism is a Crabtree positive organism, and wherein the eukaryotic organism is in a culture medium comprising excess glucose.

Preventing formation of reduced fermentation byproducts will increase the availability of both carbon and reducing equivalents for fatty alcohol, fatty aldehyde, fatty acid or isopropanol production. The two key reduced byproducts under anaerobic and microaerobic conditions are ethanol and glycerol. Ethanol is typically formed from pyruvate in two enzymatic steps catalyzed by pyruvate decarboxylase and ethanol dehydrogenase. Glycerol is formed from the glycolytic intermediate dihydroxyacetone phosphate by the enzymes glycerol-3-phsophate dehydrogenase and glycerol-3-phosphate phosphatase. Attenuation of one or more of these enzyme activities will increase the yield of fatty alcohols, fatty aldehydes, fatty acids or isopropanol. Strain engineering strategies for reducing or eliminating ethanol and glycerol formation are described herein.

Yeast such as S. cerevisiae can produce glycerol to allow for regeneration of NAD(P) under anaerobic conditions. Another way to reduce or eliminate glycerol production is by oxygen-limited cultivation (Bakker et al, supra). Glycerol formation only sets in when the specific oxygen uptake rates of the cells decrease below the rate that is required to reoxidize the NADH formed in biosynthesis.

In addition to the redox sinks listed above, malate dehydrogenase can potentially draw away reducing equivalents when it functions in the reductive direction. Several redox shuttles believed to be functional in S. cerevisiae utilize this enzyme to transfer reducing equivalents between the cytosol and the mitochondria. This transfer of redox can be prevented by attenuating malate dehydrogenase and/or malic enzyme activity. The redox shuttles that can be blocked by the attenuation of mdh include (i) malate-asparate shuttle, (ii) malate-oxaloacetate shuttle, and (iii) malate-pyruvate shuttle. Genes encoding malate dehydrogenase and malic enzymes are listed in the table below.

Protein GenBank ID GI Number Organism MDH1 NP_012838.1 6322765 Saccharomyces cerevisiae MDH2 NP_014515.2 116006499 Saccharomyces cerevisiae MDH3 NP_010205.1 6320125 Saccharomyces cerevisiae MAE1 NP_012896.1 6322823 Saccharomyces cerevisiae MDH1 XP_722674.1 68466384 Candida albicans MDH2 XP_718638.1 68474530 Candida albicans MAE1 XP_716669.1 68478574 Candida albicans KLLA0F25960g XP_456236.1 50312405 Kluyveromyces lactis KLLA0E18635g XP_454793.1 50309563 Kluyveromyces lactis KLLA0E07525g XP_454288.1 50308571 Kluyveromyces lactis YALI0D16753p XP_502909.1 50550873 Yarrowia lipolytica YALI0E18634p XP_504112.1 50553402 Yarrowia lipolytica ANI_1_268064 XP_001391302.1 145237310 Aspergillus niger ANI_1_12134 XP_001396546.1 145250065 Aspergillus niger ANI_1_22104 XP_001395105.2 317033225 Aspergillus niger

Overall, disruption or attenuation of the aforementioned sinks for redox either individually or in combination with the other redox sinks can eliminate or lower the use of reducing power for respiration or byproduct formation. It has been reported that the deletion of the external NADH dehydrogenases (NDE1 and NDE2) and the mitochondrial G3P dehydrogenase (GUT2) almost completely eliminates cytosolic NAD+regeneration in S. cerevisiae (Overkamp et al, J Bacteriol 182:2823-30 (2000)).

Microorganisms of the invention produce fatty alcohols, fatty aldehydes fatty acids or isopropanol and optionally secrete the fatty alcohols, fatty aldehydes fatty acids or isopropanol into the culture medium. S. cerevisiae, Yarrowia lipolytica and E. coli harboring heterologous fatty alcohol forming activities accumulated fatty alcohols intracellularly; however fatty alcohols were not detected in the culture medium (Behrouzian et al, United States Patent Application 20100298612). The introduction of fatty acyl-CoA reductase enzymes with improved activity resulted in higher levels of fatty alcohol secreted into the culture media. Alternately, introduction of a fatty alcohol, fatty aldehyde, fatty acid or isopropanol transporter or transport system can improve extracellular accumulation of fatty alcohols, fatty aldehydes or fatty acids. Exemplary transporters are listed in the table below.

Protein GenBank ID GI Number Organism Fatp NP_524723.2 24583463 Drosophila melanogaster AY161280.1:45..1757 AAN73268.1 34776949 Rhodococcus erythropolis acrA CAF23274.1 46399825 Candidatus Protochlamydia amoebophila acrB CAF23275.1 46399826 Candidatus Protochlamydia amoebophila CER5 AY734542.1 52354013 Arabidopsis thaliana AmiS2 JC5491 7449112 Rhodococcus sp. ANI_1_1160064 XP_001391993.1 145238692 Aspergillus niger YALI0E16016g XP_504004.1 50553188 Yarrowia lipolytica

Thus, in some embodiments, the invention provides a non-naturally occurring microbial organism as disclosed herein having one or more gene disruptions, wherein the one or more gene disruptions occur in endogenous genes encoding proteins or enzymes involved in: native production of ethanol, glycerol, acetate, formate, lactate, CO₂, fatty acids, or malonyl-CoA by said microbial organism; transfer of pathway intermediates to cellular compartments other than the cytosol; or native degradation of a MI-FAE cycle intermediate, a MD-FAE cycle intermediate, a FAACPE cycle intermediate or a termination pathway intermediate by the microbial organism, the one or more gene disruptions confer increased production of a fatty alcohol, fatty aldehyde or fatty acid in the microbial organism. Accordingly, the protein or enzyme can be a fatty acid synthase, an acetyl-CoA carboxylase, a biotin:apoenzyme ligase, an acyl carrier protein, a thioesterase, an acyltransferase, an ACP malonyltransferase, a fatty acid elongase, an acyl-CoA synthetase, an acyl-CoA transferase, an acyl-CoA hydrolase, a pyruvate decarboxylase, a lactate dehydrogenase, an alcohol dehydrogenase, an acid-forming aldehyde dehydrogenases, an acetate kinase, a phosphotransacetylase, a pyruvate oxidase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate phosphatase, a mitochondrial pyruvate carrier, a peroxisomal fatty acid transporter, a peroxisomal acyl-CoA transporter, a peroxisomal carnitine/acylcarnitine transferase, an acyl-CoA oxidase, or an acyl-CoA binding protein. In some aspects, the one or more gene disruptions include a deletion of the one or more genes.

In some embodiments, the invention provides a non-naturally occurring microbial organism as described herein, wherein one or more enzymes of the MI-FAE cycle, the MD-FAE cycle, the FAACPE cycle or the termination pathway preferentially react with an NADH cofactor or have reduced preference for reacting with an NAD(P)H cofactor. For example, the one or more enzymes of the MI-FAE cycle can be a 3-ketoacyl-CoA reductase or an enoyl-CoA reductase. As another example, the one or more enzymes of the FAACPE cycle can be a 3-ketoacyl-ACP reductase or an enoyl-ACP reductase. For the termination pathway, the one or more enzymes can be an acyl-CoA reductase (aldehyde forming), an alcohol dehydrogenase, an acyl-CoA reductase (alcohol forming), an aldehyde decarbonylase, an acyl-ACP reductase, an aldehyde dehydrogenase (acid forming) or a carboxylic acid reductase.

In some embodiments, the invention provides a non-naturally occurring microbial organism as described herein having one or more gene disruptions in genes encoding proteins or enzymes that result in an increased ratio of NAD(P)H to NAD(P) present in the cytosol of the microbial organism following the disruptions. Accordingly, the gene encoding a protein or enzyme that results in an increased ratio of NAD(P)H to NAD(P) present in the cytosol of the microbial organism following the disruptions can be an NADH dehydrogenase, a cytochrome oxidase, a G3P dehydrogenase, G3P phosphatase, an alcohol dehydrogenase, a pyruvate decarboxylase, an aldehyde dehydrogenase (acid forming), a lactate dehydrogenase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate:quinone oxidoreductase, a malic enzyme and a malate dehydrogenase. In some aspects, the one or more gene disruptions include a deletion of the one or more genes.

In some embodiments, the non-naturally occurring microbial organism of the invention is Crabtree positive and is in culture medium comprising excess glucose. In such conditions, as described herein, the microbial organism can result in increasing the ratio of NAD(P)H to NAD(P) present in the cytosol of the microbial organism.

In some embodiments, the invention provides a non-naturally occurring microbial organism as described herein having at least one exogenous nucleic acid encoding an extracellular transporter or an extracellular transport system for a fatty alcohol, fatty aldehyde or fatty acid of the invention.

In some embodiments, the invention provides a non-naturally occurring microbial organism as described herein, wherein one or more endogenous enzymes involved in: native production of ethanol, glycerol, acetate, formate, lactate, CO₂, fatty acids, or malonyl-CoA by said microbial organism; transfer of pathway intermediates to cellular compartments other than the cytosol; or native degradation of a MI-FAE cycle intermediate, a MD-FAE cycle intermediate, a FAACPE cycle intermediate or a termination pathway intermediate by said microbial organism, has attenuated enzyme activity or expression levels. Accordingly, the endogenous enzyme can be a fatty acid synthase, an acetyl-CoA carboxylase, a biotin:apoenzyme ligase, an acyl carrier protein, a thioesterase, an acyltransferase, an ACP malonyltransferase, a fatty acid elongase, an acyl-CoA synthetase, an acyl-CoA transferase, an acyl-CoA hydrolase, a pyruvate decarboxylase, a lactate dehydrogenase, an alcohol dehydrogenase, an acid-forming aldehyde dehydrogenases, an acetate kinase, a phosphotransacetylase, a pyruvate oxidase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate phosphatase, a mitochondrial pyruvate carrier, a peroxisomal fatty acid transporter, a peroxisomal acyl-CoA transporter, a peroxisomal carnitine/acylcarnitine transferase, an acyl-CoA oxidase, or an acyl-CoA binding protein.

In some embodiments, the invention provides a non-naturally occurring microbial organism as described herein, wherein one or more endogenous enzymes involved in the oxidation of NAD(P)H or NADH, has attenuated enzyme activity or expression levels. Accordingly, the one or more endogenous enzymes can be a NADH dehydrogenase, a cytochrome oxidase, a G3P dehydrogenase, G3P phosphatase, an alcohol dehydrogenase, a pyruvate decarboxylase, an aldehyde dehydrogenase (acid forming), a lactate dehydrogenase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate:quinone oxidoreductase, a malic enzyme and a malate dehydrogenase.

The non-naturally occurring microbal organisms of the invention can contain stable genetic alterations, which refers to microorganisms that can be cultured for greater than five generations without loss of the alteration. Generally, stable genetic alterations include modifications that persist greater than 10 generations, particularly stable modifications will persist more than about 25 generations, and more particularly, stable genetic modifications will be greater than 50 generations, including indefinitely.

In the case of gene disruptions, a particularly useful stable genetic alteration is a gene deletion. The use of a gene deletion to introduce a stable genetic alteration is particularly useful to reduce the likelihood of a reversion to a phenotype prior to the genetic alteration. For example, stable growth-coupled production of a biochemical can be achieved, for example, by deletion of a gene encoding an enzyme catalyzing one or more reactions within a set of metabolic modifications. The stability of growth-coupled production of a biochemical can be further enhanced through multiple deletions, significantly reducing the likelihood of multiple compensatory reversions occurring for each disrupted activity.

Also provided is a method of producing a non-naturally occurring microbial organisms having stable growth-coupled production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol. The method can include identifying in silico a set of metabolic modifications that increase production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol, for example, increase production during exponential growth; genetically modifying an organism to contain the set of metabolic modifications that increase production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol, and culturing the genetically modified organism. If desired, culturing can include adaptively evolving the genetically modified organism under conditions requiring production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol. The methods of the invention are applicable to bacterium, yeast and fungus as well as a variety of other cells and microorganism, as disclosed herein.

Thus, the invention provides a non-naturally occurring microbial organism comprising one or more gene disruptions that confer increased production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol. In one embodiment, the one or more gene disruptions confer growth-coupled production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol, and can, for example, confer stable growth-coupled production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol. In another embodiment, the one or more gene disruptions can confer obligatory coupling of fatty alcohol, fatty aldehyde, fatty acid or isopropanol production to growth of the microbial organism. Such one or more gene disruptions reduce the activity of the respective one or more encoded enzymes.

The non-naturally occurring microbial organism can have one or more gene disruptions included in a gene encoding a enzyme or protein disclosed herein. As disclosed herein, the one or more gene disruptions can be a deletion. Such non-naturally occurring microbial organisms of the invention include bacteria, yeast, fungus, or any of a variety of other microorganisms applicable to fermentation processes, as disclosed herein.

Thus, the invention provides a non-naturally occurring microbial organism, comprising one or more gene disruptions, where the one or more gene disruptions occur in genes encoding proteins or enzymes where the one or more gene disruptions confer increased production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol in the organism. The production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol can be growth-coupled or not growth-coupled. In a particular embodiment, the production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol can be obligatorily coupled to growth of the organism, as disclosed herein.

The invention provides non naturally occurring microbial organisms having genetic alterations such as gene disruptions that increase production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol, for example, growth-coupled production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol. Product production can be, for example, obligatorily linked to the exponential growth phase of the microorganism by genetically altering the metabolic pathways of the cell, as disclosed herein. The genetic alterations can increase the production of the desired product or even make the desired product an obligatory product during the growth phase. Metabolic alterations or transformations that result in increased production and elevated levels of fatty alcohol, fatty aldehyde, fatty acid or isopropanol biosynthesis are exemplified herein. Each alteration corresponds to the requisite metabolic reaction that should be functionally disrupted. Functional disruption of all reactions within one or more of the pathways can result in the increased production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol by the engineered strain during the growth phase.

Each of these non-naturally occurring alterations result in increased production and an enhanced level of fatty alcohol, fatty aldehyde, fatty acid or isopropanol production, for example, during the exponential growth phase of the microbial organism, compared to a strain that does not contain such metabolic alterations, under appropriate culture conditions. Appropriate conditions include, for example, those disclosed herein, including conditions such as particular carbon sources or reactant availabilities and/or adaptive evolution.

Given the teachings and guidance provided herein, those skilled in the art will understand that to introduce a metabolic alteration such as attenuation of an enzyme, it can be necessary to disrupt the catalytic activity of the one or more enzymes involved in the reaction. Alternatively, a metabolic alteration can include disrupting expression of a regulatory protein or cofactor necessary for enzyme activity or maximal activity. Furthermore, genetic loss of a cofactor necessary for an enzymatic reaction can also have the same effect as a disruption of the gene encoding the enzyme. Disruption can occur by a variety of methods including, for example, deletion of an encoding gene or incorporation of a genetic alteration in one or more of the encoding gene sequences. The encoding genes targeted for disruption can be one, some, or all of the genes encoding enzymes involved in the catalytic activity. For example, where a single enzyme is involved in a targeted catalytic activity, disruption can occur by a genetic alteration that reduces or eliminates the catalytic activity of the encoded gene product. Similarly, where the single enzyme is multimeric, including heteromeric, disruption can occur by a genetic alteration that reduces or destroys the function of one or all subunits of the encoded gene products. Destruction of activity can be accomplished by loss of the binding activity of one or more subunits required to form an active complex, by destruction of the catalytic subunit of the multimeric complex or by both. Other functions of multimeric protein association and activity also can be targeted in order to disrupt a metabolic reaction of the invention. Such other functions are well known to those skilled in the art. Similarly, a target enzyme activity can be reduced or eliminated by disrupting expression of a protein or enzyme that modifies and/or activates the target enzyme, for example, a molecule required to convert an apoenzyme to a holoenzyme. Further, some or all of the functions of a single polypeptide or multimeric complex can be disrupted according to the invention in order to reduce or abolish the catalytic activity of one or more enzymes involved in a reaction or metabolic modification of the invention. Similarly, some or all of enzymes involved in a reaction or metabolic modification of the invention can be disrupted so long as the targeted reaction is reduced or eliminated.

Given the teachings and guidance provided herein, those skilled in the art also will understand that an enzymatic reaction can be disrupted by reducing or eliminating reactions encoded by a common gene and/or by one or more orthologs of that gene exhibiting similar or substantially the same activity. Reduction of both the common gene and all orthologs can lead to complete abolishment of any catalytic activity of a targeted reaction. However, disruption of either the common gene or one or more orthologs can lead to a reduction in the catalytic activity of the targeted reaction sufficient to promote coupling of growth to product biosynthesis. Exemplified herein are both the common genes encoding catalytic activities for a variety of metabolic modifications as well as their orthologs. Those skilled in the art will understand that disruption of some or all of the genes encoding a enzyme of a targeted metabolic reaction can be practiced in the methods of the invention and incorporated into the non-naturally occurring microbial organisms of the invention in order to achieve the increased production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol or growth-coupled product production.

Given the teachings and guidance provided herein, those skilled in the art also will understand that enzymatic activity or expression can be attenuated using well known methods. Reduction of the activity or amount of an enzyme can mimic complete disruption of a gene if the reduction causes activity of the enzyme to fall below a critical level that is normally required for a pathway to function. Reduction of enzymatic activity by various techniques rather than use of a gene disruption can be important for an organism's viability. Methods of reducing enzymatic activity that result in similar or identical effects of a gene disruption include, but are not limited to: reducing gene transcription or translation; destabilizing mRNA, protein or catalytic RNA; and mutating a gene that affects enzyme activity or kinetics (See, Sambrook et al., Molecular Cloning: A Laboratory Manual, Third Ed., Cold Spring Harbor Laboratory, New York (2001); and Ausubel et al., Current Protocols in Molecular Biology, John Wiley and Sons, Baltimore, Md. (1999). Natural or imposed regulatory controls can also accomplish enzyme attenuation including: promoter replacement (See, Wang et al., Mol. Biotechnol. 52(2):300-308 (2012)); loss or alteration of transcription factors (Dietrick et al., Annu. Rev. Biochem. 79:563-590 (2010); and Simicevic et al., Mol. Biosyst. 6(3):462-468 (2010)); introduction of inhibitory RNAs or peptides such as siRNA, antisense RNA, RNA or peptide/small-molecule binding aptamers, ribozymes, aptazymes and riboswitches (Wieland et al., Methods 56(3):351-357 (2012); O'Sullivan, Anal. Bioanal. Chem. 372(1):44-48 (2002); and Lee et al., Curr. Opin. Biotechnol. 14(5):505-511 (2003)); and addition of drugs or other chemicals that reduce or disrupt enzymatic activity such as an enzyme inhibitor, an antibiotic or a target-specific drug.

One skilled in the art will also understand and recognize that attenuation of an enzyme can be done at various levels. For example, at the gene level, a mutation causing a partial or complete null phenotype, such as a gene disruption, or a mutation causing epistatic genetic effects that mask the activity of a gene product (Miko, Nature Education 1(1) (2008)), can be used to attenuate an enzyme. At the gene expression level, methods for attenuation include: coupling transcription to an endogenous or exogenous inducer, such as isopropylthio-β-galactoside (IPTG), then adding low amounts of inducer or no inducer during the production phase (Donovan et al., J. Ind. Microbiol. 16(3):145-154 (1996); and Hansen et al., Curr. Microbiol. 36(6):341-347 (1998)); introducing or modifying a positive or a negative regulator of a gene; modify histone acetylation/deacetylation in a eukaryotic chromosomal region where a gene is integrated (Yang et al., Curr. Opin. Genet. Dev. 13(2):143-153 (2003) and Kurdistani et al., Nat. Rev. Mol. Cell Biol. 4(4):276-284 (2003)); introducing a transposition to disrupt a promoter or a regulatory gene (Bleykasten-Brosshans et al., C. R. Biol. 33(8-9):679-686 (2011); and McCue et al., PLoS Genet. 8(2):e1002474 (2012)); flipping the orientation of a transposable element or promoter region so as to modulate gene expression of an adjacent gene (Wang et al., Genetics 120(4):875-885 (1988); Hayes, Anna. Rev. Genet 37:3-29 (2003); in a diploid organism, deleting one allele resulting in loss of heterozygosity (Daigaku et al., Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 600(1-2)177-183 (2006)); introducing nucleic acids that increase RNA degradation (Houseley et al., Cell, 136(4):763-776 (2009); or in bacteria, for example, introduction of a transfer-messenger RNA (tmRNA) tag, which can lead to RNA degradation and ribosomal stalling (Sunohara et al., RNA 10(3):378-386 (2004); and Sunohara et al., J. Biol. Chem. 279:15368-15375 (2004)). At the translational level, attenuation can include: introducing rare codons to limit translation (Angov, Biotechnol. J. 6(6):650-659 (2011)); introducing RNA interference molecules that block translation (Castel et al., Nat. Rev. Genet. 14(2):100-112 (2013); and Kawasaki et al., Curr. Opin. Mol. Ther. 7(2):125-131(2005); modifying regions outside the coding sequence, such as introducing secondary structure into an untranslated region (UTR) to block translation or reduce efficiency of translation (Ringnér et al., PLoS Comput. Biol. 1(7):e72 (2005)); adding RNAase sites for rapid transcript degradation (Pasquinelli, Nat. Rev. Genet. 13(4):271-282 (2012); and Arraiano et al., FEMS Microbiol. Rev. 34(5):883-932 (2010); introducing antisense RNA oligomers or antisense transcripts (Nashizawa et al., Front. Biosci. 17:938-958 (2012)); introducing RNA or peptide aptamers, ribozymes, aptazymes, riboswitches (Wieland et al., Methods 56(3):351-357 (2012); O'Sullivan, Anal. Bioanal. Chem. 372(1):44-48 (2002); and Lee et al., Curr. Opin. Biotechnol. 14(5):505-511(2003)); or introducing translational regulatory elements involving RNA structure that can prevent or reduce translation that can be controlled by the presence or absence of small molecules (Araujo et al., Comparative and Functional Genomics, Article ID 475731, 8 pages (2012)). At the level of enzyme localization and/or longevity, enzyme attenuation can include: adding a degradation tag for faster protein turnover (Hochstrasser, Annual Rev. Genet. 30:405-439 (1996); and Yuan et al., PLoS One 8(4):e62529 (2013)); or adding a localization tag that results in the enzyme being secreted or localized to a subcellular compartment in a eukaryotic cell, where the enzyme would not be able to react with its normal substrate (Nakai et al. Genomics 14(4):897-911 (1992); and Russell et al., J. Bact. 189(21)7581-7585 (2007)). At the level of post-translational regulation, enzyme attenuation can include: increasing intracellular concentration of known inhibitors; or modifying post-translational modified sites (Mann et al., Nature Biotech. 21:255-261 (2003)). At the level of enzyme activity, enzyme attenuation can include: adding an endogenous or an exogenous inhibitor, such as an enzyme inhibitor, an antibiotic or a target-specific drug, to reduce enzyme activity; limiting availability of essential cofactors, such as vitamin B12, for an enzyme that requires the cofactor; chelating a metal ion that is required for enzyme activity; or introducing a dominant negative mutation. The applicability of a technique for attenuation described above can depend upon whether a given host microbial organism is prokaryotic or eukaryotic, and it is understand that a determination of what is the appropriate technique for a given host can be readily made by one skilled in the art.

In some embodiments, microaerobic designs can be used based on the growth-coupled formation of the desired product. To examine this, production cones can be constructed for each strategy by first maximizing and, subsequently minimizing the product yields at different rates of biomass formation feasible in the network. If the rightmost boundary of all possible phenotypes of the mutant network is a single point, it implies that there is a unique optimum yield of the product at the maximum biomass formation rate possible in the network. In other cases, the rightmost boundary of the feasible phenotypes is a vertical line, indicating that at the point of maximum biomass the network can make any amount of the product in the calculated range, including the lowest amount at the bottommost point of the vertical line. Such designs are given a low priority.

The fatty alcohol, fatty aldehyde, fatty acid or isopropanol-production strategies identified in the various tables disclosed herein can be disrupted to increase production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol. Accordingly, the invention also provides a non-naturally occurring microbial organism having metabolic modifications coupling fatty alcohol, fatty aldehyde, fatty acid or isopropanol production to growth of the organism, where the metabolic modifications includes disruption of one or more genes selected from the genes encoding proteins and/or enzymes shown in the various tables disclosed herein.

Each of the strains can be supplemented with additional deletions if it is determined that the strain designs do not sufficiently increase the production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol and/or couple the formation of the product with biomass formation. Alternatively, some other enzymes not known to possess significant activity under the growth conditions can become active due to adaptive evolution or random mutagenesis. Such activities can also be knocked out. However, the list of gene deletion disclosed herein allows the construction of strains exhibiting high-yield production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol, including growth-coupled production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol.

In some embodiments, the invention provides a method for producing a compound of Formula (I):

wherein R₁ is C₁₋₂₄ linear alkyl; R₂ is CH₂OH, CHO, or COOH; R₃ is H, OH, or oxo (═O); and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four, comprising culturing a non-naturally occurring microbial organism described herein under conditions and for a sufficient period of time to produce the compound of Formula (I), wherein the non-naturally occurring microbial organism has one or more gene disruptions, wherein the one or more gene disruptions occur in endogenous genes encoding proteins or enzymes involved in: native production of ethanol, glycerol, acetate, formate, lactate, CO₂, fatty acids, or malonyl-CoA by said microbial organism; transfer of pathway intermediates to cellular compartments other than the cytosol; or native degradation of a MI-FAE cycle intermediate, a MD-FAE cycle intermediate or a termination pathway intermediate by the microbial organism, the one or more gene disruptions confer increased production of a fatty alcohol, fatty aldehyde or fatty acid in the microbial organism. Accordingly, the protein or enzyme can be a fatty acid synthase, an acetyl-CoA carboxylase, a biotin:apoenzyme ligase, an acyl carrier protein, a thioesterase, an acyltransferases, an ACP malonyltransferase, a fatty acid elongase, an acyl-CoA synthetase, an acyl-CoA transferase, an acyl-CoA hydrolase, a pyruvate decarboxylase, a lactate dehydrogenase, an alcohol dehydrogenase, an acid-forming aldehyde dehydrogenases, an acetate kinase, a phosphotransacetylase, a pyruvate oxidase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate phosphatase, a mitochondrial pyruvate carrier, a peroxisomal fatty acid transporters, a peroxisomal acyl-CoA transporters, a peroxisomal carnitine/acylcarnitine transferases, an acyl-CoA oxidase, or an acyl-CoA binding protein. In some aspects, the one or more gene disruptions include a deletion of the one or more genes.

In some embodiments, the invention provides a method for producing a fatty alcohol, fatty aldehyde or fatty acid using a non-naturally occurring microbial organism as described herein, wherein one or more enzymes of the MI-FAE cycle, MD-FAE cycle, FAACPE cycle or the termination pathway preferentially react with an NADH cofactor or have reduced preference for reacting with an NAD(P)H cofactor. For example, the one or more enzymes of the MI-FAE cycle or MD-FAE cycle can be a 3-ketoacyl-CoA reductase or an enoyl-CoA reductase. As another example, the one or more enzymes of the FAACPE cycle can be a 3-ketoacyl-ACP reductase or an enoyl-ACP reductase. For the termination pathway, the one or more enzymes can be an acyl-CoA reductase (aldehyde forming), an alcohol dehydrogenase, an acyl-CoA reductase (alcohol forming), an aldehyde decarbonylase, an acyl-ACP reductase, an aldehyde dehydrogenase (acid forming) or a carboxylic acid reductase.

In some embodiments, the invention provides a method for producing a fatty alcohol, fatty aldehyde or fatty acid using a non-naturally occurring microbial organism as described herein having one or more gene disruptions in genes encoding proteins or enzymes that result in an increased ratio of NAD(P)H to NAD(P) present in the cytosol of the microbial organism following the disruptions. Accordingly, the gene encoding a protein or enzyme that results in an increased ratio of NAD(P)H to NAD(P) present in the cytosol of the microbial organism following the disruptions can be an NADH dehydrogenase, a cytochrome oxidase, a glycerol-3-phosphate (G3P) dehydrogenase, a glycerol-3-phosphate (G3P) phosphatase, an alcohol dehydrogenase, a pyruvate decarboxylase, an aldehyde dehydrogenase (acid forming), a lactate dehydrogenase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate:quinone oxidoreductase, a malic enzyme and a malate dehydrogenase. In some aspects, the one or more gene disruptions include a deletion of the one or more genes.

In some embodiments, the invention provides a method for producing a fatty alcohol, fatty aldehyde or fatty acid using a non-naturally occurring microbial organism of the invention that is Crabtree positive and is in culture medium comprising excess glucose. In such conditions, as described herein, the microbial organism can result in increasing the ratio of NAD(P)H to NAD(P) present in the cytosol of the microbial organism.

In some embodiments, the invention provides a method for producing a fatty alcohol, fatty aldehyde or fatty acid using a non-naturally occurring microbial organism as described herein having at least one exogenous nucleic acid encoding an extracellular transporter or an extracellular transport system for a fatty alcohol, fatty aldehyde or fatty acid of the invention.

In some embodiments, the invention provides a method for producing a fatty alcohol, fatty aldehyde or fatty acid using a non-naturally occurring microbial organism as described herein, wherein one or more endogenous enzymes involved in: native production of ethanol, glycerol, acetate, formate, lactate, CO₂, fatty acids, or malonyl-CoA by said microbial organism; transfer of pathway intermediates to cellular compartments other than the cytosol; or native degradation of a MI-FAE cycle intermediate, a MD-FAE cycle intermediate, a FAACPE cycle intermediate or a termination pathway intermediate by said microbial organism, has attenuated enzyme activity or expression levels. Accordingly, the endogenous enzyme can be a fatty acid synthase, an acetyl-CoA carboxylase, a biotin:apoenzyme ligase, an acyl carrier protein, a thioesterase, an acyltransferase, an ACP malonyltransferase, a fatty acid elongase, an acyl-CoA synthetase, an acyl-CoA transferase, an acyl-CoA hydrolase, a pyruvate decarboxylase, a lactate dehydrogenase, an alcohol dehydrogenase, an acid-forming aldehyde dehydrogenases, an acetate kinase, a phosphotransacetylase, a pyruvate oxidase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate phosphatase, a mitochondrial pyruvate carrier, a peroxisomal fatty acid transporter, a peroxisomal acyl-CoA transporter, a peroxisomal carnitine/acylcarnitine transferase, an acyl-CoA oxidase, and an acyl-CoA binding protein.

In some embodiments, the invention provides a method for producing a fatty alcohol, fatty aldehyde or fatty acid using a non-naturally occurring microbial organism as described herein, wherein one or more endogenous enzymes involved in the oxidation of NAD(P)H or NADH, has attenuated enzyme activity or expression levels. Accordingly, the one or more endogenous enzymes can be NADH dehydrogenase, a cytochrome oxidase, a glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate phosphatase, an alcohol dehydrogenase, a pyruvate decarboxylase, an aldehyde dehydrogenase (acid forming), a lactate dehydrogenase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate:quinone oxidoreductase, a malic enzyme and a malate dehydrogenase.

A fatty alcohol, fatty aldehyde or fatty acid can be harvested or isolated at any time point during the culturing of the microbial organism, for example, in a continuous and/or near-continuous culture period, as disclosed herein. Generally, the longer the microorganisms are maintained in a continuous and/or near-continuous growth phase, the proportionally greater amount of fatty alcohol, fatty aldehyde or fatty acid can be produced.

Therefore, the invention additionally provides a method for producing fatty alcohol, fatty aldehyde or fatty acid that includes culturing a non-naturally occurring microbial organism having one or more gene disruptions, as disclosed herein. The disruptions can occur in one or more genes encoding an enzyme that increases production of fatty alcohol, fatty aldehyde or fatty acid, including optionally coupling fatty alcohol, fatty aldehyde or fatty acid production to growth of the microorganism when the gene disruption reduces or eliminates an activity of the enzyme. For example, the disruptions can confer stable growth-coupled production of fatty alcohol, fatty aldehyde or fatty acid onto the non-naturally microbial organism.

In some embodiments, the gene disruption can include a complete gene deletion. In some embodiments other methods to disrupt a gene include, for example, frameshifting by omission or addition of oligonucleotides or by mutations that render the gene inoperable. One skilled in the art will recognize the advantages of gene deletions, however, because of the stability it confers to the non-naturally occurring organism from reverting to a parental phenotype in which the gene disruption has not occurred. In particular, the gene disruptions are selected from the gene sets as disclosed herein.

Once computational predictions are made of gene sets for disruption to increase production of fatty alcohol, fatty aldehyde or fatty acid, the strains can be constructed, evolved, and tested. Gene disruptions, including gene deletions, are introduced into host organism by methods well known in the art. A particularly useful method for gene disruption is by homologous recombination, as disclosed herein.

The engineered strains can be characterized by measuring the growth rate, the substrate uptake rate, and/or the product/byproduct secretion rate. Cultures can be grown and used as inoculum for a fresh batch culture for which measurements are taken during exponential growth. The growth rate can be determined by measuring optical density using a spectrophotometer (A600). Concentrations of glucose and other organic acid byproducts in the culture supernatant can be determined by well known methods such as HPLC, GC-MS or other well known analytical methods suitable for the analysis of the desired product, as disclosed herein, and used to calculate uptake and secretion rates.

Strains containing gene disruptions can exhibit suboptimal growth rates until their metabolic networks have adjusted to their missing functionalities. To assist in this adjustment, the strains can be adaptively evolved. By subjecting the strains to adaptive evolution, cellular growth rate becomes the primary selection pressure and the mutant cells are compelled to reallocate their metabolic fluxes in order to enhance their rates of growth. This reprogramming of metabolism has been recently demonstrated for several E. coli mutants that had been adaptively evolved on various substrates to reach the growth rates predicted a priori by an in silico model (Fong and Palsson, Nat. Genet. 36:1056-1058 (2004)). The growth improvements brought about by adaptive evolution can be accompanied by enhanced rates of fatty alcohol, fatty aldehyde or fatty acid production. The strains are generally adaptively evolved in replicate, running in parallel, to account for differences in the evolutionary patterns that can be exhibited by a host organism (Fong and Palsson, Nat. Genet. 36:1056-1058 (2004); Fong et al., J. Bacteriol. 185:6400-6408 (2003); Ibarra et al., Nature 420:186-189 (2002)) that could potentially result in one strain having superior production qualities over the others. Evolutions can be run for a period of time, typically 2-6 weeks, depending upon the rate of growth improvement attained. In general, evolutions are stopped once a stable phenotype is obtained.

Following the adaptive evolution process, the new strains are characterized again by measuring the growth rate, the substrate uptake rate, and the product/byproduct secretion rate. These results are compared to the theoretical predictions by plotting actual growth and production yields along side the production envelopes from metabolic modeling. The most successful design/evolution combinations are chosen to pursue further, and are characterized in lab-scale batch and continuous fermentations. The growth-coupled biochemical production concept behind the methods disclosed herein such as OptKnock approach should also result in the generation of genetically stable overproducers. Thus, the cultures are maintained in continuous mode for an extended period of time, for example, one month or more, to evaluate long-term stability. Periodic samples can be taken to ensure that yield and productivity are maintained.

Adaptive evolution is a powerful technique that can be used to increase growth rates of mutant or engineered microbial strains, or of wild-type strains growing under unnatural environmental conditions. It is especially useful for strains designed via methods such as OptKnock, which results in growth-coupled product formation. Therefore, evolution toward optimal growing strains will indirectly optimize production as well. Unique strains of E. coli K-12 MG1655 were created through gene knockouts and adaptive evolution. (Fong and Palsson, Nat. Genet. 36:1056-1058 (2004)). In this work, all adaptive evolutionary cultures were maintained in prolonged exponential growth by serial passage of batch cultures into fresh medium before the stationary phase was reached, thus rendering growth rate as the primary selection pressure. Knockout strains were constructed and evolved on minimal medium supplemented with different carbon substrates (four for each knockout strain). Evolution cultures were carried out in duplicate or triplicate, giving a total of 50 evolution knockout strains. The evolution cultures were maintained in exponential growth until a stable growth rate was reached. The computational predictions were accurate (within 10%) at predicting the post-evolution growth rate of the knockout strains in 38 out of the 50 cases examined. Furthermore, a combination of OptKnock design with adaptive evolution has led to improved lactic acid production strains. (Fong et al., Biotechnol. Bioeng. 91:643-648 (2005)) Similar methods can be applied to the strains disclosed herein and applied to various host strains.

There are a number of developed technologies for carrying out adaptive evolution. Exemplary methods are disclosed herein. In some embodiments, optimization of a non-naturally occurring organism of the present invention includes utilizing adaptive evolution techniques to increase fatty alcohol, fatty aldehyde, fatty acid or isopropanol production and/or stability of the producing strain.

Serial culture involves repetitive transfer of a small volume of grown culture to a much larger vessel containing fresh growth medium. When the cultured organisms have grown to saturation in the new vessel, the process is repeated. This method has been used to achieve the longest demonstrations of sustained culture in the literature (Lenski and Travisano, Proc. Natl. Acad Sci. USA 91:6808-6814 (1994)) in experiments which clearly demonstrated consistent improvement in reproductive rate over a period of years. Typically, transfer of cultures is usually performed during exponential phase, so each day the transfer volume is precisely calculated to maintain exponential growth through the next 24 hour period. Manual serial dilution is inexpensive and easy to parallelize.

In continuous culture the growth of cells in a chemostat represents an extreme case of dilution in which a very high fraction of the cell population remains. As a culture grows and becomes saturated, a small proportion of the grown culture is replaced with fresh media, allowing the culture to continually grow at close to its maximum population size. Chemostats have been used to demonstrate short periods of rapid improvement in reproductive rate (Dykhuizen, Methods Enzymol. 613-631 (1993)). The potential usefulness of these devices was recognized, but traditional chemostats were unable to sustain long periods of selection for increased reproduction rate, due to the unintended selection of dilution-resistant (static) variants. These variants are able to resist dilution by adhering to the surface of the chemostat, and by doing so, outcompete less adherent individuals, including those that have higher reproductive rates, thus obviating the intended purpose of the device (Chao and Ramsdell, J. Gen. Microbiol 20:132-138 (1985)). One possible way to overcome this drawback is the implementation of a device with two growth chambers, which periodically undergo transient phases of sterilization, as described previously (Marliere and Mutzel, U.S. Pat. No. 6,686,194).

Evolugator™ is a continuous culture device developed by Evolugate, LLC (Gainesville, Fla.) and exhibits significant time and effort savings over traditional evolution techniques (de Crecy et al., Appl. Microbiol. Biotechnol. 77:489-496 (2007)). The cells are maintained in prolonged exponential growth by the serial passage of batch cultures into fresh medium before the stationary phase is attained. By automating optical density measurement and liquid handling, the Evolugator™ can perform serial transfer at high rates using large culture volumes, thus approaching the efficiency of a chemostat in evolution of cell fitness. For example, a mutant of Acinetobacter sp ADP1 deficient in a component of the translation apparatus, and having severely hampered growth, was evolved in 200 generations to 80% of the wild-type growth rate. However, in contrast to the chemostat which maintains cells in a single vessel, the machine operates by moving from one “reactor” to the next in subdivided regions of a spool of tubing, thus eliminating any selection for wall-growth. The transfer volume is adjustable, and normally set to about 50%. A drawback to this device is that it is large and costly, thus running large numbers of evolutions in parallel is not practical. Furthermore, gas addition is not well regulated, and strict anaerobic conditions are not maintained with the current device configuration. Nevertheless, this is an alternative method to adaptively evolve a production strain.

As disclosed herein, a nucleic acid encoding a desired activity of a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway can be introduced into a host organism. In some cases, it can be desirable to modify an activity of a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway enzyme or protein to increase production of fatty alcohol, fatty aldehyde, fatty acid or isopropanol. For example, known mutations that increase the activity of a protein or enzyme can be introduced into an encoding nucleic acid molecule. Additionally, optimization methods can be applied to increase the activity of an enzyme or protein and/or decrease an inhibitory activity, for example, decrease the activity of a negative regulator.

One such optimization method is directed evolution. Directed evolution is a powerful approach that involves the introduction of mutations targeted to a specific gene in order to improve and/or alter the properties of an enzyme. Improved and/or altered enzymes can be identified through the development and implementation of sensitive high-throughput screening assays that allow the automated screening of many enzyme variants (for example, >10⁴). Iterative rounds of mutagenesis and screening typically are performed to afford an enzyme with optimized properties. Computational algorithms that can help to identify areas of the gene for mutagenesis also have been developed and can significantly reduce the number of enzyme variants that need to be generated and screened. Numerous directed evolution technologies have been developed (for reviews, see Hibbert et al., Biomol. Eng 22:11-19 (2005); Huisman and Lalonde, In Biocatalysis in the pharmaceutical and biotechnology industries pgs. 717-742 (2007), Patel (ed.), CRC Press; Often and Quax. Biomol. Eng 22:1-9 (2005)₄ and Sen et al., Appl Biochem. Biotechnol 143:212-223 (2007)) to be effective at creating diverse variant libraries, and these methods have been successfully applied to the improvement of a wide range of properties across many enzyme classes. Enzyme characteristics that have been improved and/or altered by directed evolution technologies include, for example: selectivity/specificity, for conversion of non-natural substrates; temperature stability, for robust high temperature processing; pH stability, for bioprocessing under lower or higher pH conditions; substrate or product tolerance, so that high product titers can be achieved; binding (K_(m)), including broadening substrate binding to include non-natural substrates; inhibition (K_(i)), to remove inhibition by products, substrates, or key intermediates; activity (kcat), to increases enzymatic reaction rates to achieve desired flux; expression levels, to increase protein yields and overall pathway flux; oxygen stability, for operation of air sensitive enzymes under aerobic conditions; and anaerobic activity, for operation of an aerobic enzyme in the absence of oxygen.

Described below in more detail are exemplary methods that have been developed for the mutagenesis and diversification of genes to target desired properties of specific enzymes. Such methods are well known to those skilled in the art. Any of these can be used to alter and/or optimize the activity of a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway enzyme or protein.

EpPCR (Pritchard et al., J Theor. Biol. 234:497-509 (2005)) introduces random point mutations by reducing the fidelity of DNA polymerase in PCR reactions by the addition of Mn²⁺ ions, by biasing dNTP concentrations, or by other conditional variations. The five step cloning process to confine the mutagenesis to the target gene of interest involves: 1) error-prone PCR amplification of the gene of interest; 2) restriction enzyme digestion; 3) gel purification of the desired DNA fragment; 4) ligation into a vector; 5) transformation of the gene variants into a suitable host and screening of the library for improved performance. This method can generate multiple mutations in a single gene simultaneously, which can be useful to screen a larger number of potential variants having a desired activity. A high number of mutants can be generated by EpPCR, so a high-throughput screening assay or a selection method, for example, using robotics, is useful to identify those with desirable characteristics.

Error-prone Rolling Circle Amplification (epRCA) (Fujii et al., Nucleic Acids Res. 32:e145 (2004); and Fujii et al., Nat. Protoc. 1:2493-2497 (2006)) has many of the same elements as epPCR except a whole circular plasmid is used as the template and random 6-mers with exonuclease resistant thiophosphate linkages on the last 2 nucleotides are used to amplify the plasmid followed by transformation into cells in which the plasmid is re-circularized at tandem repeats. Adjusting the Mn²⁺ concentration can vary the mutation rate somewhat. This technique uses a simple error-prone, single-step method to create a full copy of the plasmid with 3-4 mutations/kbp. No restriction enzyme digestion or specific primers are required. Additionally, this method is typically available as a commercially available kit.

DNA or Family Shuffling (Stemmer, Proc Natl Acad Sci USA 91:10747-10751 (1994)); and Stemmer, Nature 370:389-391 (1994)) typically involves digestion of two or more variant genes with nucleases such as Dnase I or EndoV to generate a pool of random fragments that are reassembled by cycles of annealing and extension in the presence of DNA polymerase to create a library of chimeric genes. Fragments prime each other and recombination occurs when one copy primes another copy (template switch). This method can be used with >1 kbp DNA sequences. In addition to mutational recombinants created by fragment reassembly, this method introduces point mutations in the extension steps at a rate similar to error-prone PCR. The method can be used to remove deleterious, random and neutral mutations.

Staggered Extension (StEP) (Zhao et al., Nat. Biotechnol. 16:258-261 (1998)) entails template priming followed by repeated cycles of 2 step PCR with denaturation and very short duration of annealing/extension (as short as 5 sec). Growing fragments anneal to different templates and extend further, which is repeated until full-length sequences are made. Template switching means most resulting fragments have multiple parents. Combinations of low-fidelity polymerases (Taq and Mutazyme) reduce error-prone biases because of opposite mutational spectra.

In Random Priming Recombination (RPR) random sequence primers are used to generate many short DNA fragments complementary to different segments of the template (Shao et al., Nucleic Acids Res 26:681-683 (1998)). Base misincorporation and mispriming via epPCR give point mutations. Short DNA fragments prime one another based on homology and are recombined and reassembled into full-length by repeated thermocycling. Removal of templates prior to this step assures low parental recombinants. This method, like most others, can be performed over multiple iterations to evolve distinct properties. This technology avoids sequence bias, is independent of gene length, and requires very little parent DNA for the application.

In Heteroduplex Recombination linearized plasmid DNA is used to form heteroduplexes that are repaired by mismatch repair (Volkov et al, Nucleic Acids Res. 27:e18 (1999); and Volkov et al., Methods Enzymol 328:456-463 (2000)). The mismatch repair step is at least somewhat mutagenic. Heteroduplexes transform more efficiently than linear homoduplexes. This method is suitable for large genes and whole operons.

Random Chimeragenesis on Transient Templates (RACHITT) (Coco et al., Nat. Biotechnol. 19:354-359 (2001)) employs Dnase I fragmentation and size fractionation of single stranded DNA (ssDNA). Homologous fragments are hybridized in the absence of polymerase to a complementary ssDNA scaffold. Any overlapping unhybridized fragment ends are trimmed down by an exonuclease. Gaps between fragments are filled in and then ligated to give a pool of full-length diverse strands hybridized to the scaffold, which contains U to preclude amplification. The scaffold then is destroyed and is replaced by a new strand complementary to the diverse strand by PCR amplification. The method involves one strand (scaffold) that is from only one parent while the priming fragments derive from other genes, and the parent scaffold is selected against. Thus, no reannealing with parental fragments occurs. Overlapping fragments are trimmed with an exonuclease. Otherwise, this is conceptually similar to DNA shuffling and StEP. Therefore, there should be no siblings, few inactives, and no unshuffled parentals. This technique has advantages in that few or no parental genes are created and many more crossovers can result relative to standard DNA shuffling.

Recombined Extension on Truncated templates (RETT) entails template switching of unidirectionally growing strands from primers in the presence of unidirectional ssDNA fragments used as a pool of templates (Lee et al., J. Molec. Catalysis 26:119-129 (2003)). No DNA endonucleases are used. Unidirectional ssDNA is made by DNA polymerase with random primers or serial deletion with exonuclease. Unidirectional ssDNA are only templates and not primers. Random priming and exonucleases do not introduce sequence bias as true of enzymatic cleavage of DNA shuffling/RACHITT. RETT can be easier to optimize than StEP because it uses normal PCR conditions instead of very short extensions. Recombination occurs as a component of the PCR steps, that is, no direct shuffling. This method can also be more random than StEP due to the absence of pauses.

In Degenerate Oligonucleotide Gene Shuffling (DOGS) degenerate primers are used to control recombination between molecules; (Bergquist and Gibbs, Methods Mol. Biol 352:191-204 (2007); Bergquist et al., Biomol. Eng 22:63-72 (2005); Gibbs et al., Gene 271:13-20 (2001)) this can be used to control the tendency of other methods such as DNA shuffling to regenerate parental genes. This method can be combined with random mutagenesis (epPCR) of selected gene segments. This can be a good method to block the reformation of parental sequences. No endonucleases are needed. By adjusting input concentrations of segments made, one can bias towards a desired backbone. This method allows DNA shuffling from unrelated parents without restriction enzyme digests and allows a choice of random mutagenesis methods.

Incremental Truncation for the Creation of Hybrid Enzymes (ITCHY) creates a combinatorial library with 1 base pair deletions of a gene or gene fragment of interest (Ostermeier et al., Proc. Natl. Acad. Sci. USA 96:3562-3567 (1999); and Ostermeier et al., Nat. Biotechnol. 17:1205-1209 (1999)). Truncations are introduced in opposite direction on pieces of 2 different genes. These are ligated together and the fusions are cloned. This technique does not require homology between the 2 parental genes. When ITCHY is combined with DNA shuffling, the system is called SCRATCHY (see below). A major advantage of both is no need for homology between parental genes; for example, functional fusions between an E. coli and a human gene were created via ITCHY. When ITCHY libraries are made, all possible crossovers are captured.

Thio-Incremental Truncation for the Creation of Hybrid Enzymes (THIO-ITCHY) is similar to ITCHY except that phosphothioate dNTPs are used to generate truncations (Lutz et al., Nucleic Acids Res 29:E16 (2001)). Relative to ITCHY, THIO-ITCHY can be easier to optimize, provide more reproducibility, and adjustability.

SCRATCHY combines two methods for recombining genes, ITCHY and DNA shuffling (Lutz et al., Proc. Natl. Acad. Sci. USA 98:11248-11253 (2001)). SCRATCHY combines the best features of ITCHY and DNA shuffling. First, ITCHY is used to create a comprehensive set of fusions between fragments of genes in a DNA homology-independent fashion. This artificial family is then subjected to a DNA-shuffling step to augment the number of crossovers. Computational predictions can be used in optimization. SCRATCHY is more effective than DNA shuffling when sequence identity is below 80%.

In Random Drift Mutagenesis (RNDM) mutations are made via epPCR followed by screening/selection for those retaining usable activity (Bergquist et al., Biomol. Eng. 22:63-72 (2005)). Then, these are used in DOGS to generate recombinants with fusions between multiple active mutants or between active mutants and some other desirable parent Designed to promote isolation of neutral mutations; its purpose is to screen for retained catalytic activity whether or not this activity is higher or lower than in the original gene. RNDM is usable in high throughput assays when screening is capable of detecting activity above background. RNDM has been used as a front end to DOGS in generating diversity. The technique imposes a requirement for activity prior to shuffling or other subsequent steps; neutral drift libraries are indicated to result in higher/quicker improvements in activity from smaller libraries. Though published using epPCR, this could be applied to other large-scale mutagenesis methods.

Sequence Saturation Mutagenesis (SeSaM) is a random mutagenesis method that: 1) generates a pool of random length fragments using random incorporation of a phosphothioate nucleotide and cleavage; this pool is used as a template to 2) extend in the presence of “universal” bases such as inosine; 3) replication of an inosine-containing complement gives random base incorporation and, consequently, mutagenesis (Wong et al., Biotechnol. J. 3:74-82 (2008); Wong et al., Nucleic Acids Res. 32:e26 (2004); and Wong et al., Anal. Biochem. 341:187-189 (2005)). Using this technique it can be possible to generate a large library of mutants within 2 to 3 days using simple methods. This technique is non-directed in comparison to the mutational bias of DNA polymerases. Differences in this approach makes this technique complementary (or an alternative) to epPCR.

In Synthetic Shuffling, overlapping oligonucleotides are designed to encode “all genetic diversity in targets” and allow a very high diversity for the shuffled progeny (Ness et al., Nat. Biotechnol. 20:1251-1255 (2002)). In this technique, one can design the fragments to be shuffled. This aids in increasing the resulting diversity of the progeny. One can design sequence/codon biases to make more distantly related sequences recombine at rates approaching those observed with more closely related sequences. Additionally, the technique does not require physically possessing the template genes.

Nucleotide Exchange and Excision Technology NexT exploits a combination of dUTP incorporation followed by treatment with uracil DNA glycosylase and then piperidine to perform endpoint DNA fragmentation (Muller et al., Nucleic Acids Res. 33:e117 (2005)). The gene is reassembled using internal PCR primer extension with proofreading polymerase. The sizes for shuffling are directly controllable using varying dUPT::dTTP ratios. This is an end point reaction using simple methods for uracil incorporation and cleavage. Other nucleotide analogs, such as 8-oxo-guanine, can be used with this method. Additionally, the technique works well with very short fragments (86 bp) and has a low error rate. The chemical cleavage of DNA used in this technique results in very few unshuffled clones.

In Sequence Homology-Independent Protein Recombination (SHIPREC), a linker is used to facilitate fusion between two distantly related or unrelated genes. Nuclease treatment is used to generate a range of chimeras between the two genes. These fusions result in libraries of single-crossover hybrids (Sieber et al., Nat. Biotechnol. 19:456-460 (2001)). This produces a limited type of shuffling and a separate process is required for mutagenesis. In addition, since no homology is needed, this technique can create a library of chimeras with varying fractions of each of the two unrelated parent genes. SHIPREC was tested with a heme-binding domain of a bacterial CP450 fused to N-terminal regions of a mammalian CP450; this produced mammalian activity in a more soluble enzyme.

In Gene Site Saturation Mutagenesis™ (GSSM™) the starting materials are a supercoiled dsDNA plasmid containing an insert and two primers which are degenerate at the desired site of mutations (Kretz et al., Methods Enzymol. 388:3-11 (2004)). Primers carrying the mutation of interest, anneal to the same sequence on opposite strands of DNA. The mutation is typically in the middle of the primer and flanked on each side by approximately 20 nucleotides of correct sequence. The sequence in the primer is NNN or NNK (coding) and MNN (noncoding) (N=all 4, K=G, T, M=A, C). After extension, DpnI is used to digest dam-methylated DNA to eliminate the wild-type template. This technique explores all possible amino acid substitutions at a given locus (that is, one codon). The technique facilitates the generation of all possible replacements at a single-site with no nonsense codons and results in equal to near-equal representation of most possible alleles. This technique does not require prior knowledge of the structure, mechanism, or domains of the target enzyme. If followed by shuffling or Gene Reassembly, this technology creates a diverse library of recombinants containing all possible combinations of single-site up-mutations. The usefulness of this technology combination has been demonstrated for the successful evolution of over 50 different enzymes, and also for more than one property in a given enzyme.

Combinatorial Cassette Mutagenesis (CCM) involves the use of short oligonucleotide cassettes to replace limited regions with a large number of possible amino acid sequence alterations (Reidhaar-Olson et al. Methods Enzymol. 208:564-586 (1991); and Reidhaar-Olson et al. Science 241:53-57 (1988)). Simultaneous substitutions at two or three sites are possible using this technique. Additionally, the method tests a large multiplicity of possible sequence changes at a limited range of sites. This technique has been used to explore the information content of the lambda repressor DNA-binding domain.

Combinatorial Multiple Cassette Mutagenesis (CMCM) is essentially similar to CCM except it is employed as part of a larger program: 1) use of epPCR at high mutation rate to 2) identify hot spots and hot regions and then 3) extension by CMCM to cover a defined region of protein sequence space (Reetz et al., Angew. Chem. Int. Ed Engl. 40:3589-3591 (2001)). As with CCM, this method can test virtually all possible alterations over a target region. If used along with methods to create random mutations and shuffled genes, it provides an excellent means of generating diverse, shuffled proteins. This approach was successful in increasing, by 51-fold, the enantioselectivity of an enzyme.

In the Mutator Strains technique, conditional ts mutator plasmids allow increases of 20 to 4000-× in random and natural mutation frequency during selection and block accumulation of deleterious mutations when selection is not required (Selifonova et al., Appl. Environ. Microbiol. 67:3645-3649 (2001)). This technology is based on a plasmid-derived mutD5 gene, which encodes a mutant subunit of DNA polymerase III. This subunit binds to endogenous DNA polymerase III and compromises the proofreading ability of polymerase III in any strain that harbors the plasmid. A broad-spectrum of base substitutions and frameshift mutations occur. In order for effective use, the mutator plasmid should be removed once the desired phenotype is achieved; this is accomplished through a temperature sensitive (ts) origin of replication, which allows for plasmid curing at 41° C. It should be noted that mutator strains have been explored for quite some time (see Low et al., J. Mol. Biol. 260:359-3680 (1996)). In this technique, very high spontaneous mutation rates are observed. The conditional property minimizes non-desired background mutations. This technology could be combined with adaptive evolution to enhance mutagenesis rates and more rapidly achieve desired phenotypes.

Look-Through Mutagenesis (LTM) is a multidimensional mutagenesis method that assesses and optimizes combinatorial mutations of selected amino acids (Rajpal et al., Proc. Natl. Acad. Sci. USA 102:8466-8471 (2005)). Rather than saturating each site with all possible amino acid changes, a set of nine is chosen to cover the range of amino acid R-group chemistry. Fewer changes per site allows multiple sites to be subjected to this type of mutagenesis. A >800-fold increase in binding affinity for an antibody from low nanomolar to picomolar has been achieved through this method. This is a rational approach to minimize the number of random combinations and can increase the ability to find improved traits by greatly decreasing the numbers of clones to be screened. This has been applied to antibody engineering, specifically to increase the binding affinity and/or reduce dissociation. The technique can be combined with either screens or selections.

Gene Reassembly is a DNA shuffling method that can be applied to multiple genes at one time or to create a large library of chimeras (multiple mutations) of a single gene (Tunable GeneReassembly™ (TGR™) Technology supplied by Verenium Corporation). Typically this technology is used in combination with ultra-high-throughput screening to query the represented sequence space for desired improvements. This technique allows multiple gene recombination independent of homology. The exact number and position of cross-over events can be pre-determined using fragments designed via bioinformatic analysis. This technology leads to a very high level of diversity with virtually no parental gene reformation and a low level of inactive genes. Combined with GSSM™, a large range of mutations can be tested for improved activity. The method allows “blending” and “fine tuning” of DNA shuffling, for example, codon usage can be optimized.

In Silico Protein Design Automation (PDA) is an optimization algorithm that anchors the structurally defined protein backbone possessing a particular fold, and searches sequence space for amino acid substitutions that can stabilize the fold and overall protein energetics (Hayes et al., Proc. Natl. Acad. Sci. USA 99:15926-15931 (2002)). This technology uses in silico structure-based entropy predictions in order to search for structural tolerance toward protein amino acid variations. Statistical mechanics is applied to calculate coupling interactions at each position. Structural tolerance toward amino acid substitution is a measure of coupling. Ultimately, this technology is designed to yield desired modifications of protein properties while maintaining the integrity of structural characteristics. The method computationally assesses and allows filtering of a very large number of possible sequence variants (10⁵⁰). The choice of sequence variants to test is related to predictions based on the most favorable thermodynamics. Ostensibly only stability or properties that are linked to stability can be effectively addressed with this technology. The method has been successfully used in some therapeutic proteins, especially in engineering immunoglobulins. In silico predictions avoid testing extraordinarily large numbers of potential variants. Predictions based on existing three-dimensional structures are more likely to succeed than predictions based on hypothetical structures. This technology can readily predict and allow targeted screening of multiple simultaneous mutations, something not possible with purely experimental technologies due to exponential increases in numbers.

Iterative Saturation Mutagenesis (ISM) involves: 1) using knowledge of structure/function to choose a likely site for enzyme improvement; 2) performing saturation mutagenesis at chosen site using a mutagenesis method such as Stratagene QuikChange (Stratagene; San Diego Calif.); 3) screening/selecting for desired properties; and 4) using improved clone(s), start over at another site and continue repeating until a desired activity is achieved (Reetz et al., Nat. Protoc. 2:891-903 (2007); and Reetz et al., Angew. Chem. Int. Ed Engl. 45:7745-7751 (2006)). This is a proven methodology, which assures all possible replacements at a given position are made for screening/selection.

Any of the aforementioned methods for mutagenesis can be used alone or in any combination. Additionally, any one or combination of the directed evolution methods can be used in conjunction with adaptive evolution techniques, as described herein.

It is understood that modifications which do not substantially affect the activity of the various embodiments of this invention are also provided within the definition of the invention provided herein. Accordingly, the following examples are intended to illustrate but not limit the present invention.

Example I Formate Assimilation Pathways

This example describes enzymatic pathways for converting pyruvate to formaldehyde, and optionally in combination with producing acetyl-CoA and/or reproducing pyruvate.

Step E, FIG. 1: Formate Reductase

The conversion of formate to formaldehyde can be carried out by a formate reductase (step E, FIG. 1). A suitable enzyme for these transformations is the aryl-aldehyde dehydrogenase, or equivalently a carboxylic acid reductase, from Nocardia iowensis. Carboxylic acid reductase catalyzes the magnesium, ATP and NADPH-dependent reduction of carboxylic acids to their corresponding aldehydes (Venkitasubramanian et al., J. Biol. Chem. 282:478-485 (2007)). This enzyme, encoded by car, was cloned and functionally expressed in E. coli (Venkitasubramanian et al., J. Biol. Chem. 282:478-485 (2007)). Expression of the npt gene product improved activity of the enzyme via post-transcriptional modification. The npt gene encodes a specific phosphopantetheine transferase (PPTase) that converts the inactive apo-enzyme to the active holo-enzyme. The natural substrate of this enzyme is vanillic acid, and the enzyme exhibits broad acceptance of aromatic and aliphatic substrates (Venkitasubramanian et al., in Biocatalysis in the Pharmaceutical and Biotechnology Industires, ed. R. N. Patel, Chapter 15, pp. 425-440, CRC Press LLC, Boca Raton, Fla. (2006)). Information related to these proteins and genes is shown below.

Protein GenBank ID GI number Organism Car AAR91681.1 40796035 Nocardia iowensis (sp. NRRL 5646) Npt ABI83656.1 114848891 Nocardia iowensis (sp. NRRL 5646)

Additional car and npt genes can be identified based on sequence homology.

Protein GenBank ID GI number Organism fadD9 YP_978699.1 121638475 Mycobacterium bovis BCG BCG_2812c YP_978898.1 121638674 Mycobacterium bovis BCG nfa20150 YP_118225.1 54023983 Nocardia farcinica IFM 10152 nfa40540 YP_120266.1 54026024 Nocardia farcinica IFM 10152 SGR_6790 YP_001828302.1 182440583 Streptomyces griseus subsp. griseus NBRC 13350 SGR_665 YP_001822177.1 182434458 Streptomyces griseus subsp. griseus NBRC 13350 MSMEG_2956 YP_887275.1 118473501 Mycobacterium smegmatis MC2 155 MSMEG_5739 YP_889972.1 118469671 Mycobacterium smegmatis MC2 155 MSMEG_2648 YP_886985.1 118471293 Mycobacterium smegmatis MC2 155 MAP1040c NP_959974.1 41407138 Mycobacterium avium subsp. paratuberculosis K-10 MAP2899c NP_961833.1 41408997 Mycobacterium avium subsp. paratuberculosis K-10 MMAR_2117 YP_001850422.1 183982131 Mycobacterium marinum M MMAR_2936 YP_001851230.1 183982939 Mycobacterium marinum M MMAR_1916 YP_001850220.1 183981929 Mycobacterium marinum M TpauDRAFT_33060 ZP_04027864.1 227980601 Tsukamurella paurometabola DSM 20162 TpauDRAFT_20920 ZP_04026660.1 227979396 Tsukamurella paurometabola DSM 20162 CPCC7001_1320 ZP_05045132.1 254431429 Cyanobium PCC7001 DDBDRAFT_0187729 XP_636931.1 66806417 Dictyostelium discoideum AX4

An additional enzyme candidate found in Streptomyces griseus is encoded by the griC and griD genes. This enzyme is believed to convert 3-amino-4-hydroxybenzoic acid to 3-amino-4-hydroxybenzaldehyde as deletion of either griC or griD led to accumulation of extracellular 3-acetylamino-4-hydroxybenzoic acid, a shunt product of 3-amino-4-hydroxybenzoic acid metabolism (Suzuki, et al., J. Antibiot. 60(6):380-387 (2007)). Co-expression of griC and griD with SGR_665, an enzyme similar in sequence to the Nocardia iowensis npt, can be beneficial. Information related to these proteins and genes is shown below.

Protein GenBank ID GI number Organism griC YP_001825755.1 182438036 Streptomyces griseus subsp. griseus NBRC 13350 griD YP_001825756.1 182438037 Streptomyces griseus subsp. griseus NBRC 13350

An enzyme with similar characteristics, alpha-aminoadipate reductase (AAR, EC 1.2.1.31), participates in lysine biosynthesis pathways in some fungal species. This enzyme naturally reduces alpha-aminoadipate to alpha-aminoadipate semialdehyde. The carboxyl group is first activated through the ATP-dependent formation of an adenylate that is then reduced by NAD(P)H to yield the aldehyde and AMP. Like CAR, this enzyme utilizes magnesium and requires activation by a PPTase. Enzyme candidates for AAR and its corresponding PPTase are found in Saccharomyces cerevisiae (Morris et al., Gene 98:141-145 (1991)), Candida albicans (Guo et al., Mol. Genet. Genomics 269:271-279 (2003)), and Schizosaccharomyces pombe (Ford et al., Curr. Genet. 28:131-137 (1995)). The AAR from S. pombe exhibited significant activity when expressed in E. coli (Guo et al., Yeast 21:1279-1288 (2004)). The AAR from Penicillium chrysogenum accepts S-carboxymethyl-L-cysteine as an alternate substrate, but did not react with adipate, L-glutamate or diaminopimelate (Hijarrubia et al., J. Biol. Chem. 278:8250-8256 (2003)). The gene encoding the P. chrysogenum PPTase has not been identified to date. Information related to these proteins and genes is shown below.

Protein GenBank ID GI number Organism LYS2 AAA34747.1 171867 Saccharomyces cerevisiae LYS5 P50113.1 1708896 Saccharomyces cerevisiae LYS2 AAC02241.1 2853226 Candida albicans LYS5 AAO26020.1 28136195 Candida albicans Lys1p P40976.3 13124791 Schizosaccharomyces pombe Lys7p Q10474.1 1723561 Schizosaccharomyces pombe Lys2 CAA74300.1 3282044 Penicillium chrysogenum

Tani et al (Agric Biol Chem, 1978, 42: 63-68; Agric Biol Chem, 1974, 38: 2057-2058) showed that purified enzymes from Escherichia coli strain B could reduce the sodium salts of different organic acids (e.g. formate, glycolate, acetate, etc.) to their respective aldehydes (e.g. formaldehyde, glycoaldehyde, acetaldehyde, etc.). Of three purified enzymes examined by Tani et al (1978), only the “A” isozyme was shown to reduce formate to formaldehyde. Collectively, this group of enzymes was originally termed glycoaldehyde dehydrogenase; however, their novel reductase activity led the authors to propose the name glycolate reductase as being more appropriate (Morita et al, Agric Biol Chem, 1979, 43: 185-186). Morita et al (Agric Biol Chem, 1979, 43: 185-186) subsequently showed that glycolate reductase activity is relatively widespread among microorganisms, being found for example in: Pseudomonas, Agrobacterium, Escherichia, Flavobacterium, Micrococcus, Staphylococcus, Bacillus, and others. Without wishing to be bound by any particular theory, it is believed that some of these glycolate reductase enzymes are able to reduce formate to formaldehyde.

Any of these CAR or CAR-like enzymes can exhibit formate reductase activity or can be engineered to do so.

Step F, FIG. 1 Formate Ligase, Formate Transferase, Formate Synthetase

The acylation of formate to formyl-CoA is catalyzed by enzymes with formate transferase, synthetase, or ligase activity (Step F, FIG. 1). Formate transferase enzymes have been identified in several organisms including Escherichia coli (Toyota, et al., J Bacteriol. 2008 April; 190(7):2556-64), Oxalobacter formigenes (Toyota, et al., J Bacteriol. 2008 April; 190(7):2556-64; Baetz et al., J Bacteriol. 1990 July; 172(7):3537-40; Ricagno, et al., EMBO J. 2003 July 1; 22(13):3210-9)), and Lactobacillus acidophilus (Azcarate-Peril, et al., Appl. Environ. Microbiol. 2006 72(3) 1891-1899). Homologs exist in several other organisms Enzymes acting on the CoA-donor for formate transferase may also be expressed to ensure efficient regeneration of the CoA-donor. For example, if oxalyl-CoA is the CoA donor substrate for formate transferase, an additional transferase, synthetase, or ligase may be required to enable efficient regeneration of oxalyl-CoA from oxalate. Similarly, if succinyl-CoA or acetyl-CoA is the CoA donor substrate for formate transferase, an additional transferase, synthetase, or ligase may be required to enable efficient regeneration of succinyl-CoA from succinate or acetyl-CoA from acetate, respectively.

Protein GenBank ID GI number Organism YfdW NP_416875.1 16130306 Escherichia coli frc O06644.3 21542067 Oxalobacter formigenes frc ZP_04021099.1 227903294 Lactobacillus acidophilus

Suitable CoA-donor regeneration or formate transferase enzymes are encoded by the gene products of cat1, cat2, and cat3 of Clostridium kluyveri. These enzymes have been shown to exhibit succinyl-CoA, 4-hydroxybutyryl-CoA, and butyryl-CoA acetyltransferase activity, respectively (Seedorf et al., Proc. Natl. Acad. Sci. USA 105:2128-2133 (2008); Sohling and Gottschalk, J Bacteriol 178:871-880 (1996)) Similar CoA transferase activities are also present in Trichomonas vaginalis (van Grinsven et al., J. Biol. Chem. 283:1411-1418 (2008)) and Trypanosoma brucei (Riviere et al., J. Biol. Chem. 279:45337-45346 (2004)). Yet another transferase capable of the desired conversions is butyryl-CoA:acetoacetate CoA-transferase. Exemplary enzymes can be found in Fusobacterium nucleatum (Barker et al., J. Bacteriol. 152(1):201-7 (1982)), Clostridium SB4 (Barker et al., J. Biol. Chem. 253(4):1219-25 (1978)), and Clostridium acetobutylicum (Wiesenborn et al., Appl. Environ. Microbiol. 55(2):323-9 (1989)). Although specific gene sequences were not provided for butyryl-CoA:acetoacetate CoA-transferase in these references, the genes FN0272 and FN0273 have been annotated as a butyrate-acetoacetate CoA-transferase (Kapatral et al., J. Bact. 184(7) 2005-2018 (2002)). Homologs in Fusobacterium nucleatum such as FN1857 and FN1856 also likely have the desired acetoacetyl-CoA transferase activity. FN1857 and FN1856 are located adjacent to many other genes involved in lysine fermentation and are thus very likely to encode an acetoacetate:butyrate CoA transferase (Kreimeyer, et al., J. Biol. Chem. 282 (10) 7191-7197 (2007)). Additional candidates from Porphyrmonas gingivalis and Thermoanaerobacter tengcongensis can be identified in a similar fashion (Kreimeyer, et al., J. Biol. Chem. 282 (10) 7191-7197 (2007)). Information related to these proteins and genes is shown below.

Protein GenBank ID GI number Organism Cat1 P38946.1 729048 Clostridium kluyveri Cat2 P38942.2 1705614 Clostridium kluyveri Cat3 EDK35586.1 146349050 Clostridium kluyveri TVAG_395550 XP_001330176 123975034 Trichomonas vaginalis G3 Tb11.02.0290 XP_828352 71754875 Trypanosoma brucei FN0272 NP_603179.1 19703617 Fusobacterium nucleatum FN0273 NP_603180.1 19703618 Fusobacterium nucleatum FN1857 NP_602657.1 19705162 Fusobacterium nucleatum FN1856 NP_602656.1 19705161 Fusobacterium nucleatum PG1066 NP_905281.1 34540802 Porphyromonas gingivalis W83 PG1075 NP_905290.1 34540811 Porphyromonas gingivalis W83 TTE0720 NP_622378.1 20807207 Thermoanaerobacter tengcongensis MB4 TTE0721 NP_622379.1 20807208 Thermoanaerobacter tengcongensis MB4

Additional transferase enzymes of interest include the gene products of atoAD from E. coli (Hanai et al., Appl Environ Microbiol 73:7814-7818 (2007)), ctfAB from C. acetobutylicum (Jojima et al., Appl Microbiol Biotechnol 77:1219-1224 (2008)), and ctfAB from Clostridium saccharoperbutylacetonicum (Kosaka et al., Biosci. Biotechnol Biochem. 71:58-68 (2007)). Information related to these proteins and genes is shown below.

Protein GenBank ID GI number Organism AtoA P76459.1 2492994 Escherichia coli AtoD P76458.1 2492990 Escherichia coli CtfA NP_149326.1 15004866 Clostridium acetobutylicum CtfB NP_149327.1 15004867 Clostridium acetobutylicum CtfA AAP42564.1 31075384 Clostridium saccharoperbutyl- acetonicum CtfB AAP42565.1 31075385 Clostridium saccharoperbutyl- acetonicum

Succinyl-CoA:3-ketoacid-CoA transferase naturally converts succinate to succinyl-CoA while converting a 3-ketoacyl-CoA to a 3-ketoacid. Exemplary succinyl-CoA:3:ketoacid-CoA transferases are present in Helicobacter pylori (Corthesy-Theulaz et al., J. Biol. Chem. 272:25659-25667 (1997)), Bacillus subtilis (Stols et al., Protein. Expr. Purif. 53:396-403 (2007)), and Homo sapiens (Fukao et al., Genomics 68:144-151 (2000); Tanaka et al., Mol. Hum. Reprod. 8:16-23 (2002)). Information related to these proteins and genes is shown below.

Protein GenBank ID GI number Organism HPAG1_0676 YP_627417 108563101 Helicobacter pylori HPAG1_0677 YP_627418 108563102 Helicobacter pylori ScoA NP_391778 16080950 Bacillus subtilis ScoB NP_391777 16080949 Bacillus subtilis OXCT1 NP_000427 4557817 Homo sapiens OXCT2 NP_071403 11545841 Homo sapiens

Two additional enzymes that catalyze the activation of formate to formyl-CoA reaction are AMP-forming formyl-CoA synthetase and ADP-forming formyl-CoA synthetase. Exemplary enzymes, known to function on acetate, are found in E. coli (Brown et al., J. Gen. Microbiol. 102:327-336 (1977)), Ralstonia eutropha (Priefert and Steinbuchel, J. Bacteriol. 174:6590-6599 (1992)), Methanothermobacter thermautotrophicus (Ingram-Smith and Smith, Archaea 2:95-107 (2007)), Salmonella enterica (Gulick et al., Biochemistry 42:2866-2873 (2003)) and Saccharomyces cerevisiae (Jogl and Tong, Biochemistry 43:1425-1431 (2004)). Such enzymes may also acylate formate naturally or can be engineered to do so.

Protein GenBank ID GI Number Organism acs AAC77039.1 1790505 Escherichia coli acoE AAA21945.1 141890 Ralstonia eutropha acs1 ABC87079.1 86169671 Methanothermobacter thermautotrophicus acs1 AAL23099.1 16422835 Salmonella enterica ACS1 Q01574.2 257050994 Saccharomyces cerevisiae

ADP-forming acetyl-CoA synthetase (ACD, EC 6.2.1.13) is another candidate enzyme that couples the conversion of acyl-CoA esters to their corresponding acids with the concurrent synthesis of ATP. Several enzymes with broad substrate specificities have been described in the literature. ACD I from Archaeoglobus fulgidus, encoded by AF1211, was shown to operate on a variety of linear and branched-chain substrates including acetyl-CoA, propionyl-CoA, butyryl-CoA, acetate, propionate, butyrate, isobutyryate, isovalerate, succinate, fumarate, phenylacetate, indoleacetate (Musfeldt et al., J. Bacteriol. 184:636-644 (2002)). The enzyme from Haloarcula marismortui (annotated as a succinyl-CoA synthetase) accepts propionate, butyrate, and branched-chain acids (isovalerate and isobutyrate) as substrates, and was shown to operate in the forward and reverse directions (Brasen et al., Arch. Microbiol. 182:277-287 (2004)). The ACD encoded by PAE3250 from hyperthermophilic crenarchaeon Pyrobaculum aerophilum showed the broadest substrate range of all characterized ACDs, reacting with acetyl-CoA, isobutyryl-CoA (preferred substrate) and phenylacetyl-CoA (Brasen et al., supra (2004)). The enzymes from A. fulgidus, H. marismortui and P. aerophilum have all been cloned, functionally expressed, and characterized in E. coli (Musfeldt et al., supra; Brasen et al., supra (2004)). Additional candidates include the succinyl-CoA synthetase encoded by sucCD in E. coli (Buck et al., Biochemistry 24:6245-6252 (1985)) and the acyl-CoA ligase from Pseudomonas putida (Fernandez-Valverde et al., Appl. Environ. Microbiol. 59:1149-1154 (1993)). Such enzymes may also acylate formate naturally or can be engineered to do so. Information related to these proteins and genes is shown below.

Protein GenBank ID GI number Organism AF1211 NP_070039.1 11498810 Archaeoglobus fulgidus DSM 4304 AF1983 NP_070807.1 11499565 Archaeoglobus fulgidus DSM 4304 scs YP_135572.1 55377722 Haloarcula marismortui ATCC 43049 PAE3250 NP_560604.1 18313937 Pyrobaculum aerophilum str. IM2 sucC NP_415256.1 16128703 Escherichia coli sucD AAC73823.1 1786949 Escherichia coli paaF AAC24333.2 22711873 Pseudomonas putida

An alternative method for adding the CoA moiety to formate is to apply a pair of enzymes such as a phosphate-transferring acyltransferase and a kinase. These activities enable the net formation of formyl-CoA with the simultaneous consumption of ATP. An exemplary phosphate-transferring acyltransferase is phosphotransacetylase, encoded by pta. The pta gene from E. coli encodes an enzyme that can convert acetyl-CoA into acetyl-phosphate, and vice versa (Suzuki, T. Biochim. Biophys. Acta 191:559-569 (1969)). This enzyme can also utilize propionyl-CoA instead of acetyl-CoA forming propionate in the process (Hesslinger et al. Mol. Microbiol 27:477-492 (1998)). Homologs exist in several other organisms including Salmonella enterica and Chlamydomonas reinhardtii. Such enzymes may also phosphorylate formate naturally or can be engineered to do so.

Protein GenBank ID GI number Organism Pta NP_416800.1 16130232 Escherichia coli Pta NP_461280.1 16765665 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 PAT2 XP_001694504.1 159472743 Chlamydomonas reinhardtii PAT1 XP_001691787.1 159467202 Chlamydomonas reinhardtii

An exemplary acetate kinase is the E. coli acetate kinase, encoded by ackA (Skarstedt and Silverstein J. Biol. Chem. 251:6775-6783 (1976)). Homologs exist in several other organisms including Salmonella enterica and Chlamydomonas reinhardtii. It is likely that such enzymes naturally possess formate kinase activity or can be engineered to have this activity. Information related to these proteins and genes is shown below:

Protein GenBank ID GI number Organism AckA NP_416799.1 16130231 Escherichia coli AckA NP_461279.1 16765664 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 ACK1 XP_001694505.1 159472745 Chlamydomonas reinhardtii ACK2 XP_001691682.1 159466992 Chlamydomonas reinhardtii

The acylation of formate to formyl-CoA can also be carried out by a formate ligase. For example, the product of the LSC1 and LSC2 genes of S. cerevisiae and the sucC and sucD genes of E. coli naturally form a succinyl-CoA ligase complex that catalyzes the formation of succinyl-CoA from succinate with the concomitant consumption of one ATP, a reaction which is reversible in vivo (Grays et al., U.S. Pat. No. 5,958,745, filed Sep. 28, 1999). Such enzymes may also acylate formate naturally or can be engineered to do so. Information related to these proteins and genes is shown below.

Protein GenBank ID GI number Organism SucC NP_415256.1 16128703 Escherichia coli SucD AAC73823.1 1786949 Escherichia coli LSC1 NP_014785 6324716 Saccharomyces cerevisiae LSC2 NP_011760 6321683 Saccharomyces cerevisiae

Additional exemplary CoA-ligases include the rat dicarboxylate-CoA ligase for which the sequence is yet uncharacterized (Vamecq et al., Biochemical J. 230:683-693 (1985)), either of the two characterized phenylacetate-CoA ligases from P. chrysogenum (Lamas-Maceiras et al., Biochem. J. 395:147-155 (2005); Wang et al., Biochem Biophy Res Commun 360(2):453-458 (2007)), the phenylacetate-CoA ligase from Pseudomonas putida (Martinez-Blanco et al., J. Biol. Chem. 265:7084-7090 (1990)), and the 6-carboxyhexanoate-CoA ligase from Bacillus subtilis (Bower et. al., J. Bacteriol. 178(14):4122-4130 (1996)). Additional candidate enzymes are acetoacetyl-CoA synthetases from Mus musculus (Hasegawa et al., Biochim. Biophys. Acta 1779:414-419 (2008)) and Homo sapiens (Ohgami et al., Biochem. Pharmacol. 65:989-994 (2003)), which naturally catalyze the ATP-dependant conversion of acetoacetate into acetoacetyl-CoA. 4-Hydroxybutyryl-CoA synthetase activity has been demonstrated in Metallosphaera sedula (Berg et al., Science 318:1782-1786 (2007)). This function has been tentatively assigned to the Msed_1422 gene. Such enzymes may also acylate formate naturally or can be engineered to do so. Information related to these proteins and genes is shown below.

Protein GenBank ID GI number Organism Phl CAJ15517.1 77019264 Penicillium chrysogenum PhlB ABS19624.1 152002983 Penicillium chrysogenum PaaF AAC24333.2 22711873 Pseudomonas putida BioW NP_390902.2 50812281 Bacillus subtilis AACS NP_084486.1 21313520 Mus musculus AACS NP_076417.2 31982927 Homo sapiens Msed_1422 YP_001191504 146304188 Metallosphaera sedula

Step G, FIG. 1: Formyl-CoA Reductase

Several acyl-CoA dehydrogenases are capable of reducing an acyl-CoA (e.g., formyl-CoA) to its corresponding aldehyde (e.g., formaldehyde) (Steps F, FIG. 1). Exemplary genes that encode such enzymes include the Acinetobacter calcoaceticus acr1 encoding a fatty acyl-CoA reductase (Reiser and Somerville, J. Bacteriol. 179:2969-2975 (1997), the Acinetobacter sp. M-1 fatty acyl-CoA reductase (Ishige et al., Appl. Environ. Microbiol. 68:1192-1195 (2002), and a CoA- and NADP-dependent succinate semialdehyde dehydrogenase encoded by the sucD gene in Clostridium kluyveri (Sohling and Gottschalk, J. Bacteriol. 178:871-880 (1996); Sohling and Gottschalk, J. Bacteriol. 1778:871-880 (1996)). SucD of P. gingivalis is another succinate semialdehyde dehydrogenase (Takahashi et al., J. Bacteriol. 182:4704-4710 (2000). The enzyme acylating acetaldehyde dehydrogenase in Pseudomonas sp, encoded by bphG, is yet another candidate as it has been demonstrated to oxidize and acylate acetaldehyde, propionaldehyde, butyraldehyde, isobutyraldehyde and formaldehyde (Powlowski et al., J. Bacteriol. 175:377-385 (1993)). In addition to reducing acetyl-CoA to ethanol, the enzyme encoded by adhE in Leuconostoc mesenteroides has been shown to oxidize the branched chain compound isobutyraldehyde to isobutyryl-CoA (Kazahaya et al., J. Gen. Appl. Microbiol. 18:45-55 (1972); Koo et al., Biotechnol. Lett. 27:505-510 (2005)). Butyraldehyde dehydrogenase catalyzes a similar reaction, conversion of butyryl-CoA to butyraldehyde, in solventogenic organisms such as Clostridium saccharoperbutylacetonicum (Kosaka et al., Biosci. Biotechnol. Biochem. 71:58-68 (2007)). Additional aldehyde dehydrogenase enzyme candidates are found in Desulfatibacillum alkenivorans, Citrobacter koseri, Salmonella enterica, Lactobacillus brevis and Bacillus selenitireducens. Such enzymes may be capable of naturally converting formyl-CoA to formaldehyde or can be engineered to do so.

Protein GenBank ID GI number Organism acr1 YP_047869.1 50086355 Acinetobacter calcoaceticus acr1 AAC45217 1684886 Acinetobacter baylyi acr1 BAB85476.1 18857901 Acinetobacter sp. Strain M-1 sucD P38947.1 172046062 Clostridium kluyveri sucD NP_904963.1 34540484 Porphyromonas gingivalis bphG BAA03892.1 425213 Pseudomonas sp adhE AAV66076.1 55818563 Leuconostoc mesenteroides Bld AAP42563.1 31075383 Clostridium saccharoperbutylacetonicum Ald ACL06658.1 218764192 Desulfatibacillum alkenivorans AK-01 Ald YP_001452373 157145054 Citrobacter koseri ATCC BAA-895 pduP NP_460996.1 16765381 Salmonella enterica Typhimurium pduP ABJ64680.1 116099531 Lactobacillus brevis ATCC 367 BselDRAFT_1651 ZP_02169447 163762382 Bacillus selenitireducens MLS10

An additional enzyme type that converts an acyl-CoA to its corresponding aldehyde is malonyl-CoA reductase which transforms malonyl-CoA to malonic semialdehyde. Malonyl-CoA reductase is a key enzyme in autotrophic carbon fixation via the 3-hydroxypropionate cycle in thermoacidophilic archaeal bacteria (Berg et al., Science 318:1782-1786 (2007); Thauer, Science 318:1732-1733 (2007)). The enzyme utilizes NADPH as a cofactor and has been characterized in Metallosphaera and Sulfolobus spp (Alber et al., J. Bacteriol. 188:8551-8559 (2006); Hugler et al., J. Bacteriol. 184:2404-2410 (2002)). The enzyme is encoded by Msed_0709 in Metallosphaera sedula (Alber et al., supra (2006); Berg et al., Science 318:1782-1786 (2007)). A gene encoding a malonyl-CoA reductase from Sulfolobus tokodaii was cloned and heterologously expressed in E. coli (Alber et al., J. Bacteriol. 188:8551-8559 (2006)). This enzyme has also been shown to catalyze the conversion of methylmalonyl-CoA to its corresponding aldehyde (WO 2007/141208 (2007)). Although the aldehyde dehydrogenase functionality of these enzymes is similar to the bifunctional dehydrogenase from Chloroflexus aurantiacus, there is little sequence similarity. Both malonyl-CoA reductase enzyme candidates have high sequence similarity to aspartate-semialdehyde dehydrogenase, an enzyme catalyzing the reduction and concurrent dephosphorylation of aspartyl-4-phosphate to aspartate semialdehyde. Additional gene candidates can be found by sequence homology to proteins in other organisms including Sulfolobus solfataricus and Sulfolobus acidocaldarius and have been listed below. Yet another candidate for CoA-acylating aldehyde dehydrogenase is the ald gene from Clostridium beijerinkii (Toth et al., Appl. Environ. Microbiol. 65:4973-4980 (1999). This enzyme has been reported to reduce acetyl-CoA and butyryl-CoA to their corresponding aldehydes. This gene is very similar to eutE that encodes acetaldehyde dehydrogenase of Salmonella typhimurium and E. coli (Toth et al., supra). Such enzymes may be capable of naturally converting formyl-CoA to formaldehyde or can be engineered to do so.

Protein GenBank ID GI number Organism Msed_0709 YP_001190808.1 146303492 Metallosphaera sedula Mcr NP_378167.1 15922498 Sulfolobus tokodaii asd-2 NP_343563.1 15898958 Sulfolobus solfataricus Saci_2370 YP_256941.1 70608071 Sulfolobus acidocaldarius Ald AAT66436 9473535 Clostridium beijerinckii eutE AAA80209 687645 Salmonella typhimurium eutE P77445 2498347 Escherichia coli

Step H, FIG. 1: Formyltetrahydrofolate Synthetase

Formyltetrahydrofolate synthetase ligates formate to tetrahydrofolate at the expense of one ATP. This reaction is catalyzed by the gene product of Moth_0109 in M. thermoacetica (O'brien et al., Experientia Suppl. 26:249-262 (1976); Lovell et al., Arch. Microbiol. 149:280-285 (1988); Lovell et al., Biochemistry 29:5687-5694 (1990)), FHS in Clostridium acidurici (Whitehead and Rabinowitz, J. Bacteriol. 167:203-209 (1986); Whitehead and Rabinowitz, J. Bacteriol. 170:3255-3261 (1988), and CHY_2385 in C. hydrogenoformans (Wu et al., PLoS Genet. 1:e65 (2005). Homologs exist in C. carboxidivorans P7. This enzyme is found in several other organisms as listed below.

Protein GenBank ID GI number Organism Moth_0109 YP_428991.1 83588982 Moorella thermoacetica CHY_2385 YP_361182.1 78045024 Carboxydothermus hydrogenoformans FHS P13419.1 120562 Clostridium acidurici CcarbDRAFT_1913 ZP_05391913.1 255524966 Clostridium carboxidivorans P7 CcarbDRAFT_2946 ZP_05392946.1 255526022 Clostridium carboxidivorans P7 Dhaf_0555 ACL18622.1 219536883 Desulfitobacterium hafniense fhs YP_001393842.1 153953077 Clostridium kluyveri DSM 555 fhs YP_003781893.1 300856909 Clostridium ljungdahlii DSM 13528 MGA3_08300 EIJ83208.1 387590889 Bacillus methanolicus MGA3 PB1_13509 ZP_10132113.1 387929436 Bacillus methanolicus PB1

Steps I and J, FIG. 1: Formyltetrahydrofolate Synthetase and Methylenetetrahydrofolate Dehydrogenase

In M. thermoacetica, E. coli, and C. hydrogenoformans, methenyltetrahydrofolate cyclohydrolase and methylenetetrahydrofolate dehydrogenase are carried out by the bi-functional gene products of Moth_1516, folD, and CHY_1878, respectively (Pierce et al., Environ. Microbiol. 10:2550-2573 (2008); Wu et al., PLoS Genet. 1:e65 (2005); D'Ari and Rabinowitz, J. Biol. Chem. 266:23953-23958 (1991)). A homolog exists in C. carboxidivorans P7. Several other organisms also encode for this bifunctional protein as tabulated below.

Protein GenBank ID GI number Organism Moth_1516 YP_430368.1 83590359 Moorella thermoacetica folD NP_415062.1 16128513 Escherichia coli CHY_1878 YP_360698.1 78044829 Carboxydothermus hydrogenoformans CcarbDRAFT_2948 ZP_05392948.1 255526024 Clostridium carboxidivorans P7 folD ADK16789.1 300437022 Clostridium ljungdahlii DSM 13528 folD-2 NP_951919.1 39995968 Geobacter sulfurreducens PCA folD YP_725874.1 113867385 Ralstonia eutropha H16 folD NP_348702.1 15895353 Clostridium acetobutylicum ATCC 824 folD YP_696506.1 110800457 Clostridium perfringens MGA3_09460 EIJ83438.1 387591119 Bacillus methanolicus MGA3 PB1_14689 ZP_10132349.1 387929672 Bacillus methanolicus PB1

Steps K, FIG. 1: Formaldehyde-Forming Enzyme or Spontaneous

Methylene-THF, or active formaldehyde, will spontaneously decompose to formaldehyde and THF (Thorndike and Beck, Cancer Res. 1977, 37(4) 1125-32; Ordonez and Caraballo, Psychopharmacol Commun. 1975 1(3) 253-60; Kallen and Jencks, 1966, J Biol Chem 241(24) 5851-63). To achieve higher rates, a formaldehyde-forming enzyme can be applied. Such an activity can be obtained by engineering an enzyme that reversibly forms methylene-THF from THF and a formaldehyde donor, to release free formaldehyde. Such enzymes include glycine cleavage system enzymes which naturally transfer a formaldehyde group from methylene-THF to glycine (see Step L, FIG. 1 for candidate enzymes). Additional enzymes include serine hydroxymethyltransferase (see Step M, FIG. 1 for candidate enzymes), dimethylglycine dehydrogenase (Porter, et al., Arch Biochem Biophys. 1985, 243(2) 396-407; Brizio et al., 2004, (37) 2, 434-442), sarcosine dehydrogenase (Porter, et al., Arch Biochem Biophys. 1985, 243(2) 396-407), and dimethylglycine oxidase (Leys, et al., 2003, The EMBO Journal 22(16) 4038-4048).

Protein GenBank ID GI number Organism dmgo ZP_09278452.1 359775109 Arthrobacter globiformis dmgo YP_002778684.1 226360906 Rhodococcus opacus B4 dmgo EFY87157.1 322695347 Metarhizium acridum CQMa 102 shd AAD53398.2 5902974 Homo sapiens shd NP_446116.1 GI:25742657 Rattus norvegicus dmgdh NP_037523.2 24797151 Homo sapiens dmgdh Q63342.1 2498527 Rattus norvegicus

Step L, FIG. 1: Glycine Cleavage System

The reversible NAD(P)H-dependent conversion of 5,10-methylenetetrahydrofolate and CO₂ to glycine is catalyzed by the glycine cleavage complex, also called glycine cleavage system, composed of four protein components; P, H, T and L. The glycine cleavage complex is involved in glycine catabolism in organisms such as E. coli and glycine biosynthesis in eukaryotes (Kikuchi et al, Proc Jpn Acad Ser 84:246 (2008)). The glycine cleavage system of E. coli is encoded by four genes: gcvPHT and lpdA (Okamura et al, Eur J Biochem 216:539-48 (1993); Heil et al, Microbiol 148:2203-14 (2002)). Activity of the glycine cleavage system in the direction of glycine biosynthesis has been demonstrated in vivo in Saccharomyces cerevisiae (Maaheimo et al, Eur J Biochem 268:2464-79 (2001)). The yeast GCV is encoded by GCV1, GCV2, GCV3 and LPD1.

Protein GenBank ID GI Number Organism gcvP AAC75941.1 1789269 Escherichia coli gcvT AAC75943.1 1789272 Escherichia coli gcvH AAC75942.1 1789271 Escherichia coli lpdA AAC73227.1 1786307 Escherichia coli GCV1 NP_010302.1 6320222 Saccharomyces cerevisiae GCV2 NP_013914.1 6323843 Saccharomyces cerevisiae GCV3 NP_009355.3 269970294 Saccharomyces cerevisiae LPD1 NP_116635.1 14318501 Saccharomyces cerevisiae

Step M, FIG. 1: Serine Hydroxymethyltransferase

Conversion of glycine to serine is catalyzed by serine hydroxymethyltransferase, also called glycine hydroxymethyltranferase. This enzyme reversibly converts glycine and 5,10-methylenetetrahydrofolate to serine and THF. Serine methyltransferase has several side reactions including the reversible cleavage of 3-hydroxyacids to glycine and an aldehyde, and the hydrolysis of 5,10-methenyl-THF to 5-formyl-THF. This enzyme is encoded by glyA of E. coli (Plamann et al, Gene 22:9-18 (1983)). Serine hydroxymethyltranferase enzymes of S. cerevisiae include SHM1 (mitochondrial) and SHM2 (cytosolic) (McNeil et al, J Biol Chem 269:9155-65 (1994)). Similar enzymes have been studied in Corynebacterium glutamicum and Methylobacterium extorquens (Chistoserdova et al, J Bacteriol 176:6759-62 (1994); Schweitzer et al, J Biotechnol 139:214-21(2009)).

Protein GenBank ID GI Number Organism glyA AAC75604.1 1788902 Escherichia coli SHM1 NP_009822.2 37362622 Saccharomyces cerevisiae SHM2 NP_013159.1 6323087 Saccharomyces cerevisiae glyA AAA64456.1 496116 Methylobacterium extorquens glyA AAK60516.1 14334055 Corynebacterium glutamicum

Step N, FIG. 1: Serine Deaminase

Serine can be deaminated to pyruvate by serine deaminase. Serine deaminase enzymes are present in several organisms including Clostridium acidurici (Carter, et al., 1972, J Bacteriol., 109(2) 757-763), Escherichia coli (Cicchillo et al., 2004, J Biol Chem., 279(31) 32418-25), and Corneybacterium sp. (Netzer et al., Appl Environ Microbiol. 2004 December; 70(12):7148-55).

Protein GenBank ID GI Number Organism sdaA YP_490075.1 388477887 Escherichia coli sdaB YP_491005.1 388478813 Escherichia coli tdcG YP_491301.1 388479109 Escherichia coli tdcB YP_491307.1 388479115 Escherichia coli sdaA YP_225930.1 62390528 Corynebacterium sp.

Step O, FIG. 1: Methylenetetrahydrofolate Reductase

In M. thermoacetica, this enzyme is oxygen-sensitive and contains an iron-sulfur cluster (Clark and Ljungdahl, J. Biol. Chem. 259:10845-10849 (1984). This enzyme is encoded by metF in E. coli (Sheppard et al., J. Bacteriol. 181:718-725 (1999) and CHY_1233 in C. hydrogenoformans (Wu et al., PLoS Genet. 1:e65 (2005). The M. thermoacetica genes, and its C. hydrogenoformans counterpart, are located near the CODH/ACS gene cluster, separated by putative hydrogenase and heterodisulfide reductase genes. Some additional gene candidates found bioinformatically are listed below. In Acetobacterium woodii metF is coupled to the Rnf complex through RnfC2 (Poehlein et al, PLoS One. 7:e33439). Homologs of RnfC are found in other organisms by blast search. The Rnf complex is known to be a reversible complex (Fuchs (2011) Annu. Rev. Microbiol. 65:631-658).

Protein GenBank ID GI number Organism Moth_1191 YP_430048.1 83590039 Moorella thermoacetica Moth_1192 YP_430049.1 83590040 Moorella thermoacetica metF NP_418376.1 16131779 Escherichia coli CHY_1233 YP_360071.1 78044792 Carboxydothermus hydrogenoformans CLJU_c37610 YP_003781889.1 300856905 Clostridium ljungdahlii DSM 13528 DesfrDRAFT_3717 ZP_07335241.1 303248996 Desulfovibrio fructosovorans JJ CcarbDRAFT_2950 ZP_05392950.1 255526026 Clostridium carboxidivoransP7 Ccel74_010100023124 ZP_07633513.1 307691067 Clostridium cellulovorans 743B Cphy_3110 YP_001560205.1 160881237 Clostridium phytofermentans ISDg

Step P, FIG. 1: Acetyl-CoA Synthase

Acetyl-CoA synthase is the central enzyme of the carbonyl branch of the Wood-Ljungdahl pathway. It catalyzes the synthesis of acetyl-CoA from carbon monoxide, coenzyme A, and the methyl group from a methylated corrinoid-iron-sulfur protein. The corrinoid-iron-sulfur-protein is methylated by methyltetrahydrofolate via a methyltransferase. Expression in a foreign host entails introducing one or more of the following proteins and their corresponding activities: Methyltetrahydrofolate:corrinoid protein methyltransferase (AcsE), Corrinoid iron-sulfur protein (AcsD), Nickel-protein assembly protein (AcsF), Ferredoxin (Orf7), Acetyl-CoA synthase (AcsB and AcsC), Carbon monoxide dehydrogenase (AcsA), and Nickel-protein assembly protein (CooC).

The genes used for carbon-monoxide dehydrogenase/acetyl-CoA synthase activity typically reside in a limited region of the native genome that can be an extended operon (Ragsdale, S. W., Crit. Rev. Biochem. Mol. Biol. 39:165-195 (2004); Morton et al., J. Biol. Chem. 266:23824-23828 (1991); Roberts et al., Proc. Natl. Acad. Sci. U.S.A. 86:32-36 (1989). Each of the genes in this operon from the acetogen, M. thermoacetica, has already been cloned and expressed actively in E. coli (Morton et al. supra; Roberts et al. supra; Lu et al., J. Biol. Chem. 268:5605-5614 (1993). The protein sequences of these genes can be identified by the following GenBank accession numbers.

Protein GenBank ID GI number Organism AcsE YP_430054 83590045 Moorella thermoacetica AcsD YP_430055 83590046 Moorella thermoacetica AcsF YP_430056 83590047 Moorella thermoacetica Orf7 YP_430057 83590048 Moorella thermoacetica AcsC YP_430058 83590049 Moorella thermoacetica AcsB YP_430059 83590050 Moorella thermoacetica AcsA YP_430060 83590051 Moorella thermoacetica CooC YP_430061 83590052 Moorella thermoacetica

The hydrogenic bacterium, Carboxydothermus hydrogenoformans, can utilize carbon monoxide as a growth substrate by means of acetyl-CoA synthase (Wu et al., PLoS Genet. 1:e65 (2005)). In strain Z-2901, the acetyl-CoA synthase enzyme complex lacks carbon monoxide dehydrogenase due to a frameshift mutation (Wu et al. supra (2005)), whereas in strain DSM 6008, a functional unframeshifted full-length version of this protein has been purified (Svetlitchnyi et al., Proc. Natl. Acad. Sci. U.S.A. 101:446-451(2004)). The protein sequences of the C. hydrogenoformans genes from strain Z-2901 can be identified by the following GenBank accession numbers.

Protein GenBank ID GI number Organism AcsE YP_360065 78044202 Carboxydothermus hydrogenoformans AcsD YP_360064 78042962 Carboxydothermus hydrogenoformans AcsF YP_360063 78044060 Carboxydothermus hydrogenoformans Orf7 YP_360062 78044449 Carboxydothermus hydrogenoformans AcsC YP_360061 78043584 Carboxydothermus hydrogenoformans AcsB YP_360060 78042742 Carboxydothermus hydrogenoformans CooC YP_360059 78044249 Carboxydothermus hydrogenoformans

Homologous ACS/CODH genes can also be found in the draft genome assembly of Clostridium carboxidivorans P7.

Protein GenBank ID GI Number Organism AcsA ZP_05392944.1 255526020 Clostridium carboxidivorans P7 CooC ZP_05392945.1 255526021 Clostridium carboxidivorans P7 AcsF ZP_05392952.1 255526028 Clostridium carboxidivorans P7 AcsD ZP_05392953.1 255526029 Clostridium carboxidivorans P7 AcsC ZP_05392954.1 255526030 Clostridium carboxidivorans P7 AcsE ZP_05392955.1 255526031 Clostridium carboxidivorans P7 AcsB ZP_05392956.1 255526032 Clostridium carboxidivorans P7 Orf7 ZP_05392958.1 255526034 Clostridium carboxidivorans P7

The methanogenic archaeon, Methanosarcina acetivorans, can also grow on carbon monoxide, exhibits acetyl-CoA synthase/carbon monoxide dehydrogenase activity, and produces both acetate and formate (Lessner et al., Proc. Natl. Acad. Sci. U.S.A. 103:17921-17926 (2006)). This organism contains two sets of genes that encode ACS/CODH activity (Rother and Metcalf, Proc. Natl. Acad Sci. U.S.A. 101:16929-16934 (2004)). The protein sequences of both sets of M. acetivorans genes are identified by the following GenBank accession numbers.

Protein GenBank ID GI number Organism AcsC NP_618736 20092661 Methanosarcina acetivorans AcsD NP_618735 20092660 Methanosarcina acetivorans AcsF, CooC NP_618734 20092659 Methanosarcina acetivorans AcsB NP_618733 20092658 Methanosarcina acetivorans AcsEps NP_618732 20092657 Methanosarcina acetivorans AcsA NP_618731 20092656 Methanosarcina acetivorans AcsC NP_615961 20089886 Methanosarcina acetivorans AcsD NP_615962 20089887 Methanosarcina acetivorans AcsF, CooC NP_615963 20089888 Methanosarcina acetivorans AcsB NP_615964 20089889 Methanosarcina acetivorans AcsEps NP_615965 20089890 Methanosarcina acetivorans AcsA NP_615966 20089891 Methanosarcina acetivorans

The AcsC, AcsD, AcsB, AcsEps, and AcsA proteins are commonly referred to as the gamma, delta, beta, epsilon, and alpha subunits of the methanogenic CODH/ACS. Homologs to the epsilon encoding genes are not present in acetogens such as M. thermoacetica or hydrogenogenic bacteria such as C. hydrogenoformans. Hypotheses for the existence of two active CODH/ACS operons in M. acetivorans include catalytic properties (i.e., K_(m), V_(max), k_(cat)) that favor carboxidotrophic or aceticlastic growth or differential gene regulation enabling various stimuli to induce CODH/ACS expression (Rother et al., Arch. Microbiol. 188:463-472 (2007)).

Step Y, FIG. 1: Glyceraldehydes-3-phosphate Dehydrogenase and Enzymes of Lower Glycolysis

Enzymes comprising Step Y, G3P to PYR include: Glyceraldehyde-3-phosphate dehydrogenase; Phosphoglycerate kinase; Phosphoglyceromutase; Enolase; Pyruvate kinase or PTS-dependant substrate import.

Glyceraldehyde-3-phosphate dehydrogenase enzymes include:

NADP-dependant glyceraldehyde-3-phosphate dehydrogenase, exemplary enzymes are:

Protein GenBank ID GI Number Organism gapN AAA91091.1 642667 Streptococcus mutans NP-GAPDH AEC07555.1 330252461 Arabidopsis thaliana GAPN AAM77679.2 82469904 Triticum aestivum gapN CAI56300.1 87298962 Clostridium acetobutylicum NADP-GAPDH 2D2I_A 112490271 Synechococcus elongatus PCC 7942 NADP-GAPDH CAA62619.1 4741714 Synechococcus elongatus PCC 7942 GDP1 XP_455496.1 50310947 Kluyveromyces lactis NRRL Y-1140 HP1346 NP_208138.1 15645959 Helicobacter pylori 26695 and NAD-dependant glyceraldehyde-3-phosphate dehydrogenase, exemplary enzymes are:

Protein GenBank ID GI Number Organism TDH1 NP_012483.1 6322409 Saccharomyces cerevisiae s288c TDH2 NP_012542.1 6322468 Saccharomyces cerevisiae s288c TDH3 NP_011708.1 632163 Saccharomyces cerevisiae s288c KLLA0A11858g XP_451516.1 50303157 Kluyveromyces lactis NRRL Y-1140 KLLA0F20988g XP_456022.1 50311981 Kluyveromyces lactis NRRL Y-1140 ANI_1_256144 XP_001397496.1 145251966 Aspergillus niger CBS 513.88 YALI0C06369g XP_501515.1 50548091 Yarrowia lipolytica CTRG_05666 XP_002551368.1 255732890 Candida tropicalis MYA-3404 HPODL_1089 EFW97311.1 320583095 Hansenula polymorpha DL-1 gapA YP_490040.1 388477852 Escherichia coli

Phosphoglycerate kinase enzymes include:

Protein GenBank ID GI Number Organism PGK1 NP_009938.2 10383781 Saccharomyces cerevisiae s288c PGK BAD83658.1 57157302 Candida boidinii PGK EFW98395.1 320584184 Hansenula polymorpha DL-1 pgk EIJ77825.1 387585500 Bacillus methanolicus MGA3 pgk YP_491126.1 388478934 Escherichia coli

Phosphoglyceromutase (aka phosphoglycerate mutase) enzymes include;

Protein GenBank ID GI Number Organism GPM1 NP_012770.1 6322697 Saccharomyces cerevisiae s288c GPM2 NP_010263.1 6320183 Saccharomyces cerevisiae s288c GPM3 NP_014585.1 6324516 Saccharomyces cerevisiae s288c HPODL_1391 EFW96681.1 320582464 Hansenula polymorpha DL-1 HPODL_0376 EFW97746.1 320583533 Hansenula polymorpha DL-1 gpmI EIJ77827.1 387585502 Bacillus methanolicus MGA3 gpmA YP_489028.1 388476840 Escherichia coli gpmM AAC76636.1 1790041 Escherichia coli

Enolase (also known as phosphopyruvate hydratase and 2-phosphoglycerate dehydratase) enzymes include:

Protein GenBank ID GI Number Organism ENO1 NP_011770.3 398366315 Saccharomyces cerevisiae s288c ENO2 AAB68019.1 458897 Saccharomyces cerevisiae s288c HPODL_2596 EFW95743.1 320581523 Hansenula polymorpha DL-1 eno EIJ77828.1 387585503 Bacillus methanolicus MGA3 eno AAC75821.1 1789141 Escherichia coli

Pyruvate kinase (also known as phosphoenolpyruvate kinase and phosphoenolpyruvate kinase) or PTS-dependant substrate import enzymes include those below. Pyruvate kinase, also known as phosphoenolpyruvate synthase (EC 2.7.9.2), converts pyruvate and ATP to PEP and AMP. This enzyme is encoded by the PYK1 (Burke et al., J. Biol. Chem. 258:2193-2201 (1983)) and PYK2 (Boles et al., J. Bacteriol. 179:2987-2993 (1997)) genes in S. cerevisiae. In E. coli, this activity is catalyzed by the gene products of pykF and pykA. Note that pykA and pykF are genes encoding separate enzymes potentially capable of carrying out the PYK reaction. Selected homologs of the S. cerevisiae enzymes are also shown in the table below.

Protein GenBank ID GI Number Organism PYK1 NP_009362 6319279 Saccharomyces cerevisiae PYK2 NP_014992 6324923 Saccharomyces cerevisiae pykF NP_416191.1 16129632 Escherichia coli pykA NP_416368.1 16129807 Escherichia coli KLLA0F23397g XP_456122.1 50312181 Kluyveromyces lactis CaO19.3575 XP_714934.1 68482353 Candida albicans CaO19.11059 XP_714997.1 68482226 Candida albicans YALI0F09185p XP_505195 210075987 Yarrowia lipolytica ANI_1_1126064 XP_001391973 145238652 Aspergillus niger MGA3_03005 EIJ84220.1 387591903 Bacillus methanolicus MGA3 HPODL_1539 EFW96829.1 320582612 Hansenula polymorpha DL-1

Alternatively, Phosphoenolpyruvate phosphatase (EC 3.1.3.60) catalyzes the hydrolysis of PEP to pyruvate and phosphate. Numerous phosphatase enzymes catalyze this activity, including alkaline phosphatase (EC 3.1.3.1), acid phosphatase (EC 3.1.3.2), phosphoglycerate phosphatase (EC 3.1.3.20) and PEP phosphatase (EC 3.1.3.60). PEP phosphatase enzymes have been characterized in plants such as Vignia radiate, Bruguiera sexangula and Brassica nigra. The phytase from Aspergillus fumigates, the acid phosphatase from Homo sapiens and the alkaline phosphatase of E. coli also catalyze the hydrolysis of PEP to pyruvate (Bragger et al, Appl Microbiol Biotech 63:383-9 (2004); Hayman et al, Biochem J 261:601-9 (1989); et al, The Enzymes 3rd Ed. 4:373-415 (1971))). Similar enzymes have been characterized in Campylobacter jejuni (van Mourik et al., Microbiol. 154:584-92 (2008)), Saccharomyces cerevisiae (Oshima et al., Gene 179:171-7 (1996)) and Staphylococcus aureus (Shah and Blobel, J. Bacteriol. 94:780-1 (1967)). Enzyme engineering and/or removal of targeting sequences may be required for alkaline phosphatase enzymes to function in the cytoplasm.

Protein GenBank ID GI Number Organism phyA O00092.1 41017447 Aspergillus fumigatus Acp5 P13686.3 56757583 Homo sapiens phoA NP_414917.2 49176017 Escherichia coli phoX ZP_01072054.1 86153851 Campylobacter jejuni PHO8 AAA34871.1 172164 Saccharomyces cerevisiae SaurJH1_2706 YP_001317815.1 150395140 Staphylococcus aureus

Step Q, FIG. 1: Pyruvate Formate Lyase

Pyruvate formate-lyase (PFL, EC 2.3.1.54), encoded by pflB in E. coli, can convert pyruvate into acetyl-CoA and formate. The activity of PFL can be enhanced by an activating enzyme encoded by pflA (Knappe et al., Proc. Natl. Acad. Sci U.S.A 81:1332-1335 (1984); Wong et al., Biochemistry 32:14102-14110 (1993)). Keto-acid formate-lyase (EC 2.3.1.-), also known as 2-ketobutyrate formate-lyase (KFL) and pyruvate formate-lyase 4, is the gene product of tdcE in E. coll. This enzyme catalyzes the conversion of 2-ketobutyrate to propionyl-CoA and formate during anaerobic threonine degradation, and can also substitute for pyruvate formate-lyase in anaerobic catabolism (Simanshu et al., J Biosci. 32:1195-1206 (2007)). The enzyme is oxygen-sensitive and, like PflB, can require post-translational modification by PFL-AE to activate a glycyl radical in the active site (Hesslinger et al., Mol. Microbiol 27:477-492 (1998)). A pyruvate formate-lyase from Archaeglubus fulgidus encoded by pflD has been cloned, expressed in E. coli and characterized (Lehtio et al., Protein Eng Des Sel 17:545-552 (2004)). The crystal structures of the A. fulgidus and E. coli enzymes have been resolved (Lehtio et al., J Mol. Biol. 357:221-235 (2006); Leppanen et al., Structure. 7:733-744 (1999)). Additional PFL and PFL-AE candidates are found in Lactococcus lactis (Melchiorsen et al., Appl Microbiol Biotechnol 58:338-344 (2002)), and Streptococcus mutans (Takahashi-Abbe et al., Oral. Microbiol Immunol. 18:293-297 (2003)), Chlamydomonas reinhardtii (Hemschemeier et al., Eukagot. Cell 7:518-526 (2008b); Atteia et al., J. Biol. Chem. 281:9909-9918 (2006)) and Clostridium pasteurianum (Weidner et al., J Bacteriol. 178:2440-2444 (1996)).

Protein GenBank ID GI Number Organism pflB NP_415423 16128870 Escherichia coli pflA NP_415422.1 16128869 Escherichia coli tdcE AAT48170.1 48994926 Escherichia coli pflD NP_070278.1 11499044 Archaeglubus fulgidus Pfl CAA03993 2407931 Lactococcus lactis Pfl BAA09085 1129082 Streptococcus mutans PFL1 XP_001689719.1 159462978 Chlamydomonas reinhardtii pflA1 XP_001700657.1 159485246 Chlamydomonas reinhardtii Pfl Q46266.1 2500058 Clostridium pasteurianum Act CAA63749.1 1072362 Clostridium pasteurianum Step R, FIG. 1: Pyruvate Dehydrogenase, Pyruvate Ferredoxin Oxidoreductase, Pyruvate:nadp+Oxidoreductase

The pyruvate dehydrogenase (PDH) complex catalyzes the conversion of pyruvate to acetyl-CoA (FIG. 3H). The E. coli PDH complex is encoded by the genes aceEF and lpdA Enzyme engineering efforts have improved the E. coli PDH enzyme activity under anaerobic conditions (Kim et al., J. Bacteriol. 190:3851-3858 (2008); Kim et al., Appl. Environ. Microbiol. 73:1766-1771(2007); Zhou et al., Biotechnol. Lett. 30:335-342 (2008)). In contrast to the E. coli PDH, the B. subtilis complex is active and required for growth under anaerobic conditions (Nakano et al., 179:6749-6755 (1997)). The Klebsiella pneumoniae PDH, characterized during growth on glycerol, is also active under anaerobic conditions (Menzel et al., 56:135-142 (1997)). Crystal structures of the enzyme complex from bovine kidney (Zhou et al., 98:14802-14807 (2001)) and the E2 catalytic domain from Azotobacter vinelandii are available (Mattevi et al., Science. 255:1544-1550 (1992)). Some mammalian PDH enzymes complexes can react on alternate substrates such as 2-oxobutanoate. Comparative kinetics of Rattus norvegicus PDH and BCKAD indicate that BCKAD has higher activity on 2-oxobutanoate as a substrate (Paxton et al., Biochem. J. 234:295-303 (1986)). The S. cerevisiae PDH complex canconsist of an E2 (LAT1) core that binds E1 (PDA1, PDB1), E3 (LPD1), and Protein X (PDX1) components (Pronk et al., Yeast 12:1607-1633 (1996)). The PDH complex of S. cerevisiae is regulated by phosphorylation of E1 involving PKP1 (PDH kinase I), PTC5 (PDH phosphatase I), PKP2 and PTC6. Modification of these regulators may also enhance PDH activity. Coexpression of lipoyl ligase (LplA of E. coli and AIM422 in S. cerevisiae) with PDH in the cytosol may be necessary for activating the PDH enzyme complex. Increasing the supply of cytosolic lipoate, either by modifying a metabolic pathway or media supplementation with lipoate, may also improve PDH activity.

Gene Accession No. GI Number Organism aceE NP_414656.1 16128107 Escherichia coli aceF NP_414657.1 16128108 Escherichia coli lpd NP_414658.1 16128109 Escherichia coli lplA NP_418803.1 16132203 Escherichia coli pdhA P21881.1 3123238 Bacillus subtilis pdhB P21882.1 129068 Bacillus subtilis pdhC P21883.2 129054 Bacillus subtilis pdhD P21880.1 118672 Bacillus subtilis aceE YP_001333808.1 152968699 Klebsiella pneumoniae aceF YP_001333809.1 152968700 Klebsiella pneumoniae lpdA YP_001333810.1 152968701 Klebsiella pneumoniae Pdha1 NP_001004072.2 124430510 Rattus norvegicus Pdha2 NP_446446.1 16758900 Rattus norvegicus Dlat NP_112287.1 78365255 Rattus norvegicus Dld NP_955417.1 40786469 Rattus norvegicus LAT1 NP_014328 6324258 Saccharomyces cerevisiae PDA1 NP_011105 37362644 Saccharomyces cerevisiae PDB1 NP_009780 6319698 Saccharomyces cerevisiae LPD1 NP_116635 14318501 Saccharomyces cerevisiae PDX1 NP_011709 6321632 Saccharomyces cerevisiae AIM22 NP_012489.2 83578101 Saccharomyces cerevisiae

As an alternative to the large multienzyme PDH complexes described above, some organisms utilize enzymes in the 2-ketoacid oxidoreductase family (OFOR) to catalyze acylating oxidative decarboxylation of 2-keto-acids. Unlike the PDH complexes, PFOR enzymes contain iron-sulfur clusters, utilize different cofactors and use ferredoxin or flavodixin as electron acceptors in lieu of NAD(P)H. Pyruvate ferredoxin oxidoreductase (PFOR) can catalyze the oxidation of pyruvate to form acetyl-CoA (FIG. 3H). The PFOR from Desulfovibrio africanus has been cloned and expressed in E. coli resulting in an active recombinant enzyme that was stable for several days in the presence of oxygen (Pieulle et al., J Bacteriol. 179:5684-5692 (1997)). Oxygen stability is relatively uncommon in PFORs and is believed to be conferred by a 60 residue extension in the polypeptide chain of the D. africanus enzyme. The M. thermoacetica PFOR is also well characterized (Menon et al., Biochemistry 36:8484-8494 (1997)) and was even shown to have high activity in the direction of pyruvate synthesis during autotrophic growth (Furdui et al., J Biol Chem. 275:28494-28499 (2000)). Further, E. coli possesses an uncharacterized open reading frame, ydbK, that encodes a protein that is 51% identical to the M. thermoacetica PFOR. Evidence for pyruvate oxidoreductase activity in E. coli has been described (Blaschkowski et al., Eur. J Biochem. 123:563-569 (1982)). Several additional PFOR enzymes are described in Ragsdale, Chem. Rev. 103:2333-2346 (2003). Finally, flavodoxin reductases (e.g., fqrB from Helicobacter pylori or Campylobacter jejuni (St Maurice et al., J. Bacteriol. 189:4764-4773 (2007))) or Rnf-type proteins (Seedorf et al., Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133 (2008); Herrmann et al., J. Bacteriol. 190:784-791 (2008)) provide a means to generate NADH or NADPH from the reduced ferredoxin generated by PFOR. These proteins are identified below.

Protein GenBank ID GI Number Organism Por CAA70873.1 1770208 Desulfovibrio africanus Por YP_428946.1 83588937 Moorella thermoacetica ydbK NP_415896.1 16129339 Escherichia coli fqrB NP_207955.1 15645778 Helicobacter pylori fqrB YP_001482096.1 157414840 Campylobacter jejuni RnfC EDK33306.1 146346770 Clostridium kluyveri RnfD EDK33307.1 146346771 Clostridium kluyveri RnfG EDK33308.1 146346772 Clostridium kluyveri RnfE EDK33309.1 146346773 Clostridium kluyveri RnfA EDK33310.1 146346774 Clostridium kluyveri RnfB EDK33311.1 146346775 Clostridium kluyveri

Pyruvate:NADP oxidoreductase (PNO) catalyzes the conversion of pyruvate to acetyl-CoA. This enzyme is encoded by a single gene and the active enzyme is a homodimer, in contrast to the multi-subunit PDH enzyme complexes described above. The enzyme from Euglena gracilis is stabilized by its cofactor, thiamin pyrophosphate (Nakazawa et al, Arch Biochem Biophys 411:183-8 (2003)). The mitochondrial targeting sequence of this enzyme should be removed for expression in the cytosol. The PNO protein of E. gracilis and other NADP-dependant pyruvate:NADP+ oxidoreductase enzymes are listed in the table below.

Protein GenBank ID GI Number Organism PNO Q94IN5.1 33112418 Euglena gracilis cgd4_690 XP_625673.1 66356990 Cryptosporidium parvum Iowa II TPP_PFOR_PNO XP_002765111.11 294867463 Perkinsus marinus ATCC 50983

Step S, FIG. 1: Formate Dehydrogenase

Formate dehydrogenase (FDH) catalyzes the reversible transfer of electrons from formate to an acceptor. Enzymes with FDH activity utilize various electron carriers such as, for example, NADH (EC 1.2.1.2), NADPH (EC 1.2.1.43), quinols (EC 1.1.5.6), cytochromes (EC 1.2.2.3) and hydrogenases (EC 1.1.99.33). FDH enzymes have been characterized from Moorella thermoacetica (Andreesen and Ljungdahl, J Bacteriol 116:867-873 (1973); Li et al., J Bacteriol 92:405-412 (1966); Yamamoto et al., J Biol Chem. 258:1826-1832 (1983). The loci, Moth 2312 is responsible for encoding the alpha subunit of formate dehydrogenase while the beta subunit is encoded by Moth 2314 (Pierce et al., Environ Microbiol (2008)). Another set of genes encoding formate dehydrogenase activity with a propensity for CO₂ reduction is encoded by Sfum_2703 through Sfum_2706 in Syntrophobacter fumaroxidans (de Bok et al., Eur J Biochem. 270:2476-2485 (2003)); Reda et al., PNAS 105:10654-10658 (2008)). A similar set of genes presumed to carry out the same function are encoded by CHY_0731, CHY_0732, and CHY_0733 in C. hydrogenoformans (Wu et al., PLoS Genet 1:e65 (2005)). Formate dehydrogenases are also found many additional organisms including C. carboxidivorans P7, Bacillus methanolicus, Burkholderia stabilis, Moorella thermoacetica ATCC 39073, Candida boidinii, Candida methylica, and Saccharomyces cerevisiae S288c. The soluble formate dehydrogenase from Ralstonia eutropha reduces NAD⁺ (fdsG, -B, -A, -C, -D) (Oh and Bowien, 1998)

Several EM8 enzymes have been identified that have higher specificity for NADP as the cofactor as compared to NAD. This enzyme has been deemed as the NADP-dependent formate dehydrogenase and has been reported from 5 species of the Burkholderia cepacia complex. It was tested and verified in multiple strains of Burkholderia multivorans, Burkholderia stabilis, Burkholderia pyrrocinia, and Burkholderia cenocepacia (Hatrongjit et al., Enzyme and Microbial Tech., 46: 557-561(2010)). The enzyme from Burkholderia stabilis has been characterized and the apparent K_(m) of the enzyme were reported to be 55.5 mM, 0.16 mM and 1.43 mM for formate, NADP, and NAD respectively. More gene candidates can be identified using sequence homology of proteins deposited in Public databases such as NCBI, JGI and the metagenomic databases.

Protein GenBank ID GI Number Organism Moth_2312 YP_431142 148283121 Moorella thermoacetica Moth_2314 YP_431144 83591135 Moorella thermoacetica Sfum_2703 YP_846816.1 116750129 Syntrophobacter fumaroxidans Sfum_2704 YP_846817.1 116750130 Syntrophobacter fumaroxidans Sfum_2705 YP_846818.1 116750131 Syntrophobacter fumaroxidans Sfum_2706 YP_846819.1 116750132 Syntrophobacter fumaroxidans CHY_0731 YP_359585.1 78044572 Carboxydothermus hydrogenoformans CHY_0732 YP_359586.1 78044500 Carboxydothermus hydrogenoformans CHY_0733 YP_359587.1 78044647 Carboxydothermus hydrogenoformans CcarbDRAFT_0901 ZP_05390901.1 255523938 Clostridium carboxidivorans P7 CcarbDRAFT_4380 ZP_05394380.1 255527512 Clostridium carboxidivorans P7 fdhA, MGA3_06625 EIJ82879.1 387590560 Bacillus methanolicus MGA3 fdhA, PB1_11719 ZP_10131761.1 387929084 Bacillus methanolicus PB1 fdhD, MGA3_06630 EIJ82880.1 387590561 Bacillus methanolicus MGA3 fdhD, PB1_H724 ZP_10131762.1 387929085 Bacillus methanolicus PB1 fdh ACF35003.1 194220249 Burkholderia stabilis fdh ACF35004.1 194220251 Burkholderia pyrrocinia fdh ACF35002.1 194220247 Burkholderia cenocepacia fdh ACF35001.1 194220245 Burkholderia multivorans fdh ACF35000.1 194220243 Burkholderia cepacia FDH1 AAC49766.1 2276465 Candida boidinii fdh CAA57036.1 1181204 Candida methylica FDH2 P0CF35.1 294956522 Saccharomyces cerevisiae S288c FDH1 NP_015033.1 6324964 Saccharomyces cerevisiae S288c fdsG YP_725156.1 113866667 Ralstonia eutropha fdsB YP_725157.1 113866668 Ralstonia eutropha fdsA YP_725158.1 113866669 Ralstonia eutropha fdsC YP_725159.1 113866670 Ralstonia eutropha fdsD YP_725160.1 113866671 Ralstonia eutropha

Example II Production of Reducing Equivalents

This example describes methanol metabolic pathways and other additional enzymes generating reducing equivalents as shown in FIG. 10.

FIG. 10, Step A—Methanol Methyltransferase

A complex of 3-methyltransferase proteins, denoted MtaA, MtaB, and MtaC, perform the desired methanol methyltransferase activity (Sauer et al., Eur. J. Biochem. 243:670-677 (1997); Naidu and Ragsdale, J. Bacteriol. 183:3276-3281(2001); Tallant and Krzycki, J. Biol. Chem. 276:4485-4493 (2001); Tallant and Krzycki, J. Bacteriol. 179:6902-6911(1997); Tallant and Krzycki, J. Bacteriol. 178:1295-1301(1996); Ragsdale, S. W., Crit. Rev. Biochem. Mol. Biol. 39:165-195 (2004)).

MtaB is a zinc protein that can catalyze the transfer of a methyl group from methanol to MtaC, a corrinoid protein. Exemplary genes encoding MtaB and MtaC can be found in methanogenic archaea such as Methanosarcina barkeri (Maeder et al., J. Bacteriol. 188:7922-7931(2006) and Methanosarcina acetivorans (Galagan et al., Genome Res. 12:532-542 (2002), as well as the acetogen, Moorella thermoacetica (Das et al., Proteins 67:167-176 (2007). In general, the MtaB and MtaC genes are adjacent to one another on the chromosome as their activities are tightly interdependent. The protein sequences of various MtaB and MtaC encoding genes in M. barkeri, M. acetivorans, and M. thermoaceticum can be identified by their following GenBank accession numbers.

Protein GenBank ID GI number Organism MtaB1 YP_304299 73668284 Methanosarcina barkeri MtaC1 YP_304298 73668283 Methanosarcina barkeri MtaB2 YP_307082 73671067 Methanosarcina barkeri MtaC2 YP_307081 73671066 Methanosarcina barkeri MtaB3 YP_304612 73668597 Methanosarcina barkeri MtaC3 YP_304611 73668596 Methanosarcina barkeri MtaB1 NP_615421 20089346 Methanosarcina acetivorans MtaB1 NP_615422 20089347 Methanosarcina acetivorans MtaB2 NP_619254 20093179 Methanosarcina acetivorans MtaC2 NP_619253 20093178 Methanosarcina acetivorans MtaB3 NP_616549 20090474 Methanosarcina acetivorans MtaC3 NP_616550 20090475 Methanosarcina acetivorans MtaB YP_430066 83590057 Moorella thermoacetica MtaC YP_430065 83590056 Moorella thermoacetica MtaA YP_430064 83590056 Moorella thermoacetica

The MtaB1 and MtaC1 genes, YP_304299 and YP_304298, from M. barkeri were cloned into E. coli and sequenced (Sauer et al., Eur. J. Biochem. 243:670-677 (1997)). The crystal structure of this methanol-cobalamin methyltransferase complex is also available (Hagemeier et al., Proc. Natl. Acad. Sci. U.S.A. 103:18917-18922 (2006)). The MtaB genes, YP_307082 and YP_304612, in M. barkeri were identified by sequence homology to YP_304299. In general, homology searches are an effective means of identifying methanol methyltransferases because MtaB encoding genes show little or no similarity to methyltransferases that act on alternative substrates such as trimethylamine, dimethylamine, monomethylamine, or dimethylsulfide. The MtaC genes, YP_307081 and YP_304611 were identified based on their proximity to the MtaB genes and also their homology to YP_304298. The three sets of MtaB and MtaC genes from M. acetivorans have been genetically, physiologically, and biochemically characterized (Pritchett and Metcalf, Mol. Microbiol. 56:1183-1194 (2005)). Mutant strains lacking two of the sets were able to grow on methanol, whereas a strain lacking all three sets of MtaB and MtaC genes sets could not grow on methanol. This suggests that each set of genes plays a role in methanol utilization. The M. thermoacetica MtaB gene was identified based on homology to the methanogenic MtaB genes and also by its adjacent chromosomal proximity to the methanol-induced corrinoid protein, MtaC, which has been crystallized (Zhou et al., Acta Crystallogr. Sect. F. Struct. Biol. Cyst. Commun. 61:537-540 (2005) and further characterized by Northern hybridization and Western Blotting ((Das et al., Proteins 67:167-176 (2007)).

MtaA is zinc protein that catalyzes the transfer of the methyl group from MtaC to either Coenzyme M in methanogens or methyltetrahydrofolate in acetogens. MtaA can also utilize methylcobalamin as the methyl donor. Exemplary genes encoding MtaA can be found in methanogenic archaea such as Methanosarcina barkeri (Maeder et al., J. Bacteriol. 188:7922-7931(2006) and Methanosarcina acetivorans (Galagan et al., Genome Res. 12:532-542 (2002), as well as the acetogen, Moorella thermoacetica ((Das et al., Proteins 67:167-176 (2007)). In general, MtaA proteins that catalyze the transfer of the methyl group from CH₃—MtaC are difficult to identify bioinformatically as they share similarity to other corrinoid protein methyltransferases and are not oriented adjacent to the MtaB and MtaC genes on the chromosomes. Nevertheless, a number of MtaA encoding genes have been characterized. The protein sequences of these genes in M. barkeri and M. acetivorans can be identified by the following GenBank accession numbers.

Protein GenBank ID GI number Organism MtaA YP_304602 73668587 Methanosarcina barkeri MtaA1 NP_619241 20093166 Methanosarcina acetivorans MtaA2 NP_616548 20090473 Methanosarcina acetivorans

The MtaA gene, YP_304602, from M. barkeri was cloned, sequenced, and functionally overexpressed in E. coli (Harms and Thauer, Eur. J. Biochem. 235:653-659 (1996)). In M. acetivorans, MtaA1 is required for growth on methanol, whereas MtaA2 is dispensable even though methane production from methanol is reduced in MtaA2 mutants (Bose et al., J. Bacteriol. 190:4017-4026 (2008)). There are multiple additional MtaA homologs in M. barkeri and M. acetivorans that are as yet uncharacterized, but may also catalyze corrinoid protein methyltransferase activity.

Putative MtaA encoding genes in M. thermoacetica were identified by their sequence similarity to the characterized methanogenic MtaA genes. Specifically, three M. thermoacetica genes show high homology (>30% sequence identity) to YP_304602 from M. barkeri. Unlike methanogenic MtaA proteins that naturally catalyze the transfer of the methyl group from CH₃—MtaC to Coenzyme M, an M. thermoacetica MtaA is likely to transfer the methyl group to methyltetrahydrofolate given the similar roles of methyltetrahydrofolate and Coenzyme M in methanogens and acetogens, respectively. The protein sequences of putative MtaA encoding genes from M. thermoacetica can be identified by the following GenBank accession numbers.

Protein GenBank ID GI number Organism MtaA YP_430937 83590928 Moorella thermoacetica MtaA YP_431175 83591166 Moorella thermoacetica MtaA YP_430935 83590926 Moorella thermoacetica MtaA YP_430064 83590056 Moorella thermoacetica

FIG. 10, Step B—Methylenetetrahydrofolate Reductase

The conversion of methyl-THF to methylenetetrahydrofolate is catalyzed by methylenetetrahydrofolate reductase. In M. thermoacetica, this enzyme is oxygen-sensitive and contains an iron-sulfur cluster (Clark and Ljungdahl, J. Biol. Chem. 259:10845-10849 (1984). This enzyme is encoded by metF in E. coli (Sheppard et al., J. Bacteriol. 181:718-725 (1999) and CHY_1233 in C. hydrogenoformans (Wu et al., PLoS Genet. 1:e65 (2005). The M. thermoacetica genes, and its C. hydrogenoformans counterpart, are located near the CODH/ACS gene cluster, separated by putative hydrogenase and heterodisulfide reductase genes. Some additional gene candidates found bioinformatically are listed below. In Acetobacterium woodii metF is coupled to the Rnf complex through RnfC2 (Poehlein et al, PLoS One. 7:e33439). Homologs of RnfC are found in other organisms by blast search. The Rnf complex is known to be a reversible complex (Fuchs (2011) Annu. Rev. Microbiol. 65:631-658).

Protein GenBank ID GI number Organism Moth_1191 YP_430048.1 83590039 Moorella thermoacetica Moth_1192 YP_430049.1 83590040 Moorella thermoacetica metF NP_418376.1 16131779 Escherichia coli CHY_1233 YP_360071.1 78044792 Carboxydothermus hydrogenoformans CLJU_c37610 YP_003781889.1 300856905 Clostridium ljungdahlii DSM 13528 DesfrDRAFT_3717 ZP_07335241.1 303248996 Desulfovibrio fructosovorans JJ CcarbDRAFT_2950 ZP_05392950.1 255526026 Clostridium carboxidivorans P7 Ccel74_010100023124 ZP_07633513.1 307691067 Clostridium cellulovorans 743B Cphy_3110 YP_001560205.1 160881237 Clostridium phytofermentans ISDg

FIG. 10, Steps C and D—Methylenetetrahydrofolate Dehydrogenase, Methenyltetrahydrofolate Cyclohydrolase

In M. thermoacetica, E. coli, and C. hydrogenoformans, methenyltetrahydrofolate cyclohydrolase and methylenetetrahydrofolate dehydrogenase are carried out by the bi-functional gene products of Moth_1516, folD, and CHY_1878, respectively (Pierce et al., Environ. Microbiol. 10:2550-2573 (2008); Wu et al., PLoS Genet. 1:e65 (2005); D'Ari and Rabinowitz, J. Biol. Chem. 266:23953-23958 (1991)). A homolog exists in C. carboxidivorans P7. Several other organisms also encode for this bifunctional protein as tabulated below.

Protein GenBank ID GI number Organism Moth_1516 YP_430368.1 83590359 Moorella thermoacetica folD NP_415062.1 16128513 Escherichia coli CHY_1878 YP_360698.1 78044829 Carboxydothermus hydrogenoformans CcarbDRAFT_2948 ZP_05392948.1 255526024 Clostridium carboxidivorans P7 folD ADK16789.1 300437022 Clostridium ljungdahlii DSM 13528 folD-2 NP_951919.1 39995968 Geobacter sulfurreducens PCA folD YP_725874.1 113867385 Ralstonia eutropha H16 folD NP_348702.1 15895353 Clostridium acetobutylicum ATCC 824 folD YP_696506.1 110800457 Clostridium perfringens MGA3_09460 EIJ83438.1 387591119 Bacillus methanolicus MGA3 PB1_14689 ZP_10132349.1 387929672 Bacillus methanolicus PB1

FIG. 10, Step E—Formyltetrahydrofolate Deformylase

This enzyme catalyzes the hydrolysis of 10-formyltetrahydrofolate (formyl-THF) to THF and formate. In E. coli, this enzyme is encoded by purU and has been overproduced, purified, and characterized (Nagy, et al., J. Bacteriol. 3:1292-1298 (1995)). Homologs exist in Corynebacterium sp. U-96 (Suzuki, et al., Biosci. Biotechnol. Biochem. 69(5):952-956 (2005)), Corynebacterium glutamicum ATCC 14067, Salmonella enterica, and several additional organisms.

Protein GenBank ID GI number Organism purU AAC74314.1 1787483 Escherichia coli K-12 MG1655 purU BAD97821.1 63002616 Corynebacterium sp. U-96 purU EHE84645.1 354511740 Corynebacterium glutamicum ATCC 14067 purU NP_460715.1 16765100 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

FIG. 10, Step F—Formyltetrahydrofolate Synthetase

Formyltetrahydrofolate synthetase ligates formate to tetrahydrofolate at the expense of one ATP. This reaction is catalyzed by the gene product of Moth_0109 in M. thermoacetica (O'brien et al., Experientia Suppl. 26:249-262 (1976); Lovell et al., Arch. Microbiol. 149:280-285 (1988); Lovell et al., Biochemistry 29:5687-5694 (1990)), FHS in Clostridium acidurici (Whitehead and Rabinowitz, J. Bacteriol. 167:203-209 (1986); Whitehead and Rabinowitz, J. Bacteriol. 170:3255-3261 (1988), and CHY_2385 in C. hydrogenoformans (Wu et al., PLoS Genet. 1:e65 (2005). Homologs exist in C. carboxidivorans P7. This enzyme is found in several other organisms as listed below.

Protein GenBank ID GI number Organism Moth_0109 YP_428991.1 83588982 Moorella thermoacetica CHY_2385 YP_361182.1 78045024 Carboxydothermus hydrogenoformans FHS P13419.1 120562 Clostridium acidurici CcarbDRAFT_1913 ZP_05391913.1 255524966 Clostridium carboxidivorans P7 CcarbDRAFT_2946 ZP_05392946.1 255526022 Clostridium carboxidivorans P7 Dhaf_0555 ACL18622.1 219536883 Desulfitobacterium hafniense fhs YP_001393842.1 153953077 Clostridium kluyveri DSM 555 fhs YP_003781893.1 300856909 Clostridium ljungdahlii DSM 13528 MGA3_08300 EIJ83208.1 387590889 Bacillus methanolicus MGA3 PB1_13509 ZP_10132113.1 387929436 Bacillus methanolicus PB1

FIG. 10, Step G—Formate Hydrogen Lyase

A formate hydrogen lyase enzyme can be employed to convert formate to carbon dioxide and hydrogen. An exemplary formate hydrogen lyase enzyme can be found in Escherichia coli. The E. coli formate hydrogen lyase consists of hydrogenase 3 and formate dehydrogenase-H (Maeda et al., Appl Microbiol Biotechnol 77:879-890 (2007)). It is activated by the gene product of fhlA. (Maeda et al., Appl Microbiol Biotechnol 77:879-890 (2007)). The addition of the trace elements, selenium, nickel and molybdenum, to a fermentation broth has been shown to enhance formate hydrogen lyase activity (Soini et al., Microb. Cell Fact. 7:26 (2008)). Various hydrogenase 3, formate dehydrogenase and transcriptional activator genes are shown below.

Protein GenBank ID GI number Organism hycA NP_417205 16130632 Escherichia coli K-12 MG1655 hycB NP_417204 16130631 Escherichia coli K-12 MG1655 hycC NP_417203 16130630 Escherichia coli K-12 MG1655 hycD NP_417202 16130629 Escherichia coli K-12 MG1655 hycE NP_417201 16130628 Escherichia coli K-12 MG1655 hycF NP_417200 16130627 Escherichia coli K-12 MG1655 hycG NP_417199 16130626 Escherichia coli K-12 MG1655 hycH NP_417198 16130625 Escherichia coli K-12 MG1655 hycI NP_417197 16130624 Escherichia coli K-12 MG1655 fdhF NP_418503 16131905 Escherichia coli K-12 MG1655 fhlA NP_417211 16130638 Escherichia coli K-12 MG1655

A formate hydrogen lyase enzyme also exists in the hyperthermophilic archaeon, Thermococcus litoralis (Takacs et al., BMC. Microbiol 8:88 (2008)).

Protein GenBank ID GI number Organism mhyC ABW05543 157954626 Thermococcus litoralis mhyD ABW05544 157954627 Thermococcus litoralis mhyE ABW05545 157954628 Thermococcus litoralis myhF ABW05546 157954629 Thermococcus litoralis myhG ABW05547 157954630 Thermococcus litoralis myhH ABW05548 157954631 Thermococcus litoralis fdhA AAB94932 2746736 Thermococcus litoralis fdhB AAB94931 157954625 Thermococcus litoralis

Additional formate hydrogen lyase systems have been found in Salmonella typhimurium, Klebsiella pneumoniae, Rhodospirillum rubrum, Methanobacterium formicicum (Vardar-Schara et al., Microbial Biotechnology 1:107-125 (2008)).

FIG. 10, Step H—Hydrogenase

Hydrogenase enzymes can convert hydrogen gas to protons and transfer electrons to acceptors such as ferredoxins, NAD+, or NADP+. Ralstonia eutropha H16 uses hydrogen as an energy source with oxygen as a terminal electron acceptor. Its membrane-bound uptake [NiFe]-hydrogenase is an “02-tolerant” hydrogenase (Cracknell, et al. Proc Nat Acad Sci, 106(49) 20681-20686 (2009)) that is periplasmically-oriented and connected to the respiratory chain via a b-type cytochrome (Schink and Schlegel, Biochim. Biophys. Acta, 567, 315-324 (1979); Bernhard et al., Eur. J. Biochem. 248, 179-186 (1997)). R. eutropha also contains an O₂-tolerant soluble hydrogenase encoded by the Hox operon which is cytoplasmic and directly reduces NAD+ at the expense of hydrogen (Schneider and Schlegel, Biochim. Biophys. Acta 452, 66-80 (1976); Burgdorf J. Bact. 187(9) 3122-3132(2005)). Soluble hydrogenase enzymes are additionally present in several other organisms including Geobacter sulfurreducens (Coppi, Microbiology 151, 1239-1254 (2005)), Synechocystis str. PCC 6803 (Germer, J. Biol. Chem., 284(52), 36462-36472 (2009)), and Thiocapsa roseopersicina (Rakhely, Appl. Environ. Microbiol. 70(2) 722-728 (2004)). The Synechocystis enzyme is capable of generating NADPH from hydrogen. Overexpression of both the Hox operon from Synechocystis str. PCC 6803 and the accessory genes encoded by the Hyp operon from Nostoc sp. PCC 7120 led to increased hydrogenase activity compared to expression of the Hox genes alone (Germer, J. Biol. Chem. 284(52), 36462-36472 (2009)).

Protein GenBank ID GI Number Organism HoxF NP_942727.1 38637753 Ralstonia eutropha H16 HoxU NP_942728.1 38637754 Ralstonia eutropha H16 HoxY NP_942729.1 38637755 Ralstonia eutropha H16 HoxH NP_942730.1 38637756 Ralstonia eutropha H16 HoxW NP_942731.1 38637757 Ralstonia eutropha H16 HoxI NP_942732.1 38637758 Ralstonia eutropha H16 HoxE NP_953767.1 39997816 Geobacter sulfurreducens HoxF NP_953766.1 39997815 Geobacter sulfurreducens HoxU NP_953765.1 39997814 Geobacter sulfurreducens HoxY NP_953764.1 39997813 Geobacter sulfurreducens HoxH NP_953763.1 39997812 Geobacter sulfurreducens GSU2717 NP_953762.1 39997811 Geobacter sulfurreducens HoxE NP_441418.1 16330690 Synechocystis str. PCC 6803 HoxF NP_441417.1 16330689 Synechocystis str. PCC 6803 Unknown NP_441416.1 16330688 Synechocystis str. PCC 6803 function HoxU NP_441415.1 16330687 Synechocystis str. PCC 6803 HoxY NP_441414.1 16330686 Synechocystis str. PCC 6803 Unknown NP_441413.1 16330685 Synechocystis str. PCC 6803 function Unknown NP_441412.1 16330684 Synechocystis str. PCC 6803 function HoxH NP_441411.1 16330683 Synechocystis str. PCC 6803 HypF NP_484737.1 17228189 Nostoc sp. PCC 7120 HypC NP_484738.1 17228190 Nostoc sp. PCC 7120 HypD NP_484739.1 17228191 Nostoc sp. PCC 7120 Unknown NP_484740.1 17228192 Nostoc sp. PCC 7120 function HypE NP_484741.1 17228193 Nostoc sp. PCC 7120 HypA NP_484742.1 17228194 Nostoc sp. PCC 7120 HypB NP_484743.1 17228195 Nostoc sp. PCC 7120 Hox1E AAP50519.1 37787351 Thiocapsa roseopersicina Hox1F AAP50520.1 37787352 Thiocapsa roseopersicina Hox1U AAP50521.1 37787353 Thiocapsa roseopersicina Hox1Y AAP50522.1 37787354 Thiocapsa roseopersicina Hox1H AAP50523.1 37787355 Thiocapsa roseopersicina

The genomes of E. coli and other enteric bacteria encode up to four hydrogenase enzymes (Sawers, G., Antonie Van Leeuwenhoek 66:57-88 (1994); Sawers et al., J Bacteriol. 164:1324-1331 (1985); Sawers and Boxer, Eur. J Biochem. 156:265-275 (1986); Sawers et al., J Bacteriol. 168:398-404 (1986)). Given the multiplicity of enzyme activities E. coli or another host organism can provide sufficient hydrogenase activity to split incoming molecular hydrogen and reduce the corresponding acceptor. Endogenous hydrogen-lyase enzymes of E. coli include hydrogenase 3, a membrane-bound enzyme complex using ferredoxin as an acceptor, and hydrogenase 4 that also uses a ferredoxin acceptor. Hydrogenase 3 and 4 are encoded by the hyc and hyfgene clusters, respectively. Hydrogenase activity in E. coli is also dependent upon the expression of the hyp genes whose corresponding proteins are involved in the assembly of the hydrogenase complexes (Jacobi et al., Arch. Microbiol 158:444-451 (1992); Rangarajan et al., J Bacteriol. 190:1447-1458 (2008)). The M. thermoacetica and Clostridium ljungdahli hydrogenases are suitable for a host that lacks sufficient endogenous hydrogenase activity. M. thermoacetica and C. ljungdahli can grow with CO₂ as the exclusive carbon source indicating that reducing equivalents are extracted from H₂ to enable acetyl-CoA synthesis via the Wood-Ljungdahl pathway (Drake, H. L., J Bacteriol. 150:702-709 (1982); Drake and Daniel, Res Microbiol 155:869-883 (2004); Kellum and Drake, J Bacteriol. 160:466-469 (1984)). M. thermoacetica has homologs to several hyp, hyc, and hyfgenes from E. coli. These protein sequences encoded for by these genes are identified by the following GenBank accession numbers. In addition, several gene clusters encoding hydrogenase functionality are present in M. thermoacetica and C. ljungdahli (see for example US 2012/0003652).

Protein GenBank ID GI Number Organism HypA NP_417206 16130633 Escherichia coli HypB NP_417207 16130634 Escherichia coli HypC NP_417208 16130635 Escherichia coli HypD NP_417209 16130636 Escherichia coli HypE NP_417210 226524740 Escherichia coli HypF NP_417192 16130619 Escherichia coli HycA NP_417205 16130632 Escherichia coli HycB NP_417204 16130631 Escherichia coli HycC NP_417203 16130630 Escherichia coli HycD NP_417202 16130629 Escherichia coli HycE NP_417201 16130628 Escherichia coli HycF NP_417200 16130627 Escherichia coli HycG NP_417199 16130626 Escherichia coli HycH NP_417198 16130625 Escherichia coli HycI NP_417197 16130624 Escherichia coli HyfA NP_416976 90111444 Escherichia coli HyfB NP_416977 16130407 Escherichia coli HyfC NP_416978 90111445 Escherichia coli HyfD NP_416979 16130409 Escherichia coli HyfE NP_416980 16130410 Escherichia coli HyfF NP_416981 16130411 Escherichia coli HyfG NP_416982 16130412 Escherichia coli HyfH NP_416983 16130413 Escherichia coli HyfI NP_416984 16130414 Escherichia coli HyfJ NP_416985 90111446 Escherichia coli HyfR NP_416986 90111447 Escherichia coli

Proteins in M. thermoacetica whose genes are homologous to the E. coli hydrogenase genes are shown below.

Protein GenBank ID GI Number Organism Moth_2175 YP_431007 83590998 Moorella thermoacetica Moth_2176 YP_431008 83590999 Moorella thermoacetica Moth_2177 YP_431009 83591000 Moorella thermoacetica Moth_2178 YP_431010 83591001 Moorella thermoacetica Moth_2179 YP_431011 83591002 Moorella thermoacetica Moth_2180 YP_431012 83591003 Moorella thermoacetica Moth_2181 YP_431013 83591004 Moorella thermoacetica Moth_2182 YP_431014 83591005 Moorella thermoacetica Moth_2183 YP_431015 83591006 Moorella thermoacetica Moth_2184 YP_431016 83591007 Moorella thermoacetica Moth_2185 YP_431017 83591008 Moorella thermoacetica Moth_2186 YP_431018 83591009 Moorella thermoacetica Moth_2187 YP_431019 83591010 Moorella thermoacetica Moth_2188 YP_431020 83591011 Moorella thermoacetica Moth_2189 YP_431021 83591012 Moorella thermoacetica Moth_2190 YP_431022 83591013 Moorella thermoacetica Moth_2191 YP_431023 83591014 Moorella thermoacetica Moth_2192 YP_431024 83591015 Moorella thermoacetica Moth_0439 YP_429313 83589304 Moorella thermoacetica Moth_0440 YP_429314 83589305 Moorella thermoacetica Moth_0441 YP_429315 83589306 Moorella thermoacetica Moth_0442 YP_429316 83589307 Moorella thermoacetica Moth_0809 YP_429670 83589661 Moorella thermoacetica Moth_0810 YP_429671 83589662 Moorella thermoacetica Moth_0811 YP_429672 83589663 Moorella thermoacetica Moth_0812 YP_429673 83589664 Moorella thermoacetica Moth_0814 YP_429674 83589665 Moorella thermoacetica Moth_0815 YP_429675 83589666 Moorella thermoacetica Moth_0816 YP_429676 83589667 Moorella thermoacetica Moth_1193 YP_430050 83590041 Moorella thermoacetica Moth_1194 YP_430051 83590042 Moorella thermoacetica Moth_1195 YP_430052 83590043 Moorella thermoacetica Moth_1196 YP_430053 83590044 Moorella thermoacetica Moth_1717 YP_430562 83590553 Moorella thermoacetica Moth_1718 YP_430563 83590554 Moorella thermoacetica Moth_1719 YP_430564 83590555 Moorella thermoacetica Moth_1883 YP_430726 83590717 Moorella thermoacetica Moth_1884 YP_430727 83590718 Moorella thermoacetica Moth_1885 YP_430728 83590719 Moorella thermoacetica Moth_1886 YP_430729 83590720 Moorella thermoacetica Moth_1887 YP_430730 83590721 Moorella thermoacetica Moth_1888 YP_430731 83590722 Moorella thermoacetica Moth_1452 YP_430305 83590296 Moorella thermoacetica Moth_1453 YP_430306 83590297 Moorella thermoacetica Moth_1454 YP_430307 83590298 Moorella thermoacetica

Genes encoding hydrogenase enzymes from C. ljungdahli are shown below.

Protein GenBank ID GI Number Organism CLJU_c20290 ADK15091.1 300435324 Clostridium ljungdahli CLJU_c07030 ADK13773.1 300434006 Clostridium ljungdahli CLJU_c07040 ADK13774.1 300434007 Clostridium ljungdahli CLJU_c07050 ADK13775.1 300434008 Clostridium ljungdahli CLJU_c07060 ADK13776.1 300434009 Clostridium ljungdahli CLJU_c07070 ADK13777.1 300434010 Clostridium ljungdahli CLJU_c07080 ADK13778.1 300434011 Clostridium ljungdahli CLJU_c14730 ADK14541.1 300434774 Clostridium ljungdahli CLJU_c14720 ADK14540.1 300434773 Clostridium ljungdahli CLJU_c14710 ADK14539.1 300434772 Clostridium ljungdahli CLJU_c14700 ADK14538.1 300434771 Clostridium ljungdahli CLJU_c28670 ADK15915.1 300436148 Clostridium ljungdahli CLJU_c28660 ADK15914.1 300436147 Clostridium ljungdahli CLJU_c28650 ADK15913.1 300436146 Clostridium ljungdahli CLJU_c28640 ADK15912.1 300436145 Clostridium ljungdahli

In some cases, hydrogenase encoding genes are located adjacent to a CODH. In Rhodospirillum rubrum, the encoded CODH/hydrogenase proteins form a membrane-bound enzyme complex that has been indicated to be a site where energy, in the form of a proton gradient, is generated from the conversion of CO and H₂O to CO₂ and H₂ (Fox et al., J Bacteriol. 178:6200-6208 (1996)). The CODH-I of C. hydrogenoformans and its adjacent genes have been proposed to catalyze a similar functional role based on their similarity to the R. rubrum CODH/hydrogenase gene cluster (Wu et al., PLoS Genet. 1:e65 (2005)). The C. hydrogenoformans CODH-I was also shown to exhibit intense CO oxidation and CO₂ reduction activities when linked to an electrode (Parkin et al., J Am. Chem. Soc. 129:10328-10329 (2007)).

Protein GenBank ID GI Number Organism CooL AAC45118 1515468 Rhodospirillum rubrum CooX AAC45119 1515469 Rhodospirillum rubrum CooU AAC45120 1515470 Rhodospirillum rubrum CooH AAC45121 1498746 Rhodospirillum rubrum CooF AAC45122 1498747 Rhodospirillum rubrum CODH (CooS) AAC45123 1498748 Rhodospirillum rubrum CooC AAC45124 1498749 Rhodospirillum rubrum CooT AAC45125 1498750 Rhodospirillum rubrum CooJ AAC45126 1498751 Rhodospirillum rubrum CODH-I YP_360644 78043418 Carboxydothermus (CooS-I) hydrogenoformans CooF YP_360645 78044791 Carboxydothermus hydrogenoformans HypA YP_360646 78044340 Carboxydothermus hydrogenoformans CooH YP_360647 78043871 Carboxydothermus hydrogenoformans CooU YP_360648 78044023 Carboxydothermus hydrogenoformans CooX YP_360649 78043124 Carboxydothermus hydrogenoformans CooL YP_360650 78043938 Carboxydothermus hydrogenoformans CooK YP_360651 78044700 Carboxydothermus hydrogenoformans CooM YP_360652 78043942 Carboxydothermus hydrogenoformans CooC YP_360654.1 78043296 Carboxydothermus hydrogenoformans CooA-1 YP_360655.1 78044021 Carboxydothermus hydrogenoformans

Some hydrogenase and CODH enzymes transfer electrons to ferredoxins. Ferredoxins are small acidic proteins containing one or more iron-sulfur clusters that function as intracellular electron carriers with a low reduction potential. Reduced ferredoxins donate electrons to Fe-dependent enzymes such as ferredoxin-NADP⁺ oxidoreductase, pyruvate:ferredoxin oxidoreductase (PFOR) and 2-oxoglutarate:ferredoxin oxidoreductase (OFOR). The H. thermophilus gene fdxI encodes a [4Fe-4S]-type ferredoxin that is required for the reversible carboxylation of 2-oxoglutarate and pyruvate by OFOR and PFOR, respectively (Yamamoto et al., Extremophiles 14:79-85 (2010)). The ferredoxin associated with the Sulfolobus solfataricus 2-oxoacid:ferredoxin reductase is a monomeric dicluster [3Fe-4S][4Fe-4S] type ferredoxin (Park et al. 2006). While the gene associated with this protein has not been fully sequenced, the N-terminal domain shares 93% homology with the zfx ferredoxin from S. acidocaldarius. The E. coli genome encodes a soluble ferredoxin of unknown physiological function, fdx. Some evidence indicates that this protein can function in iron-sulfur cluster assembly (Takahashi and Nakamura, 1999). Additional ferredoxin proteins have been characterized in Helicobacter pylori (Mukhopadhyay et al. 2003) and Campylobacter jejuni (van Vliet et al. 2001). A 2Fe-2S ferredoxin from Clostridium pasteurianum has been cloned and expressed in E. coli (Fujinaga and Meyer, Biochemical and Biophysical Research Communications, 192(3): (1993)). Acetogenic bacteria such as Moorella thermoacetica, Clostridium carboxidivorans P7, Clostridium ljungdahli and Rhodospirillum rubrum are predicted to encode several ferredoxins, listed below.

Protein GenBank ID GI Number Organism fdx1 BAE02673.1 68163284 Hydrogenobacter thermophilus M11214.1 AAA83524.1 144806 Clostridium pasteurianum Zfx AAY79867.1 68566938 Sulfolobus acidocalarius Fdx AAC75578.1 1788874 Escherichia coli hp_0277 AAD07340.1 2313367 Helicobacter pylori fdxA CAL34484.1 112359698 Campylobacter jejuni Moth_0061 ABC18400.1 83571848 Moorella thermoacetica Moth_1200 ABC19514.1 83572962 Moorella thermoacetica Moth_1888 ABC20188.1 83573636 Moorella thermoacetica Moth_2112 ABC20404.1 83573852 Moorella thermoacetica Moth_1037 ABC19351.1 83572799 Moorella thermoacetica CcarbDRAFT_4383 ZP_05394383.1 255527515 Clostridium carboxidivorans P7 CcarbDRAFT_2958 ZP_05392958.1 255526034 Clostridium carboxidivorans P7 CcarbDRAFT_2281 ZP_05392281.1 255525342 Clostridium carboxidivorans P7 CcarbDRAFT_5296 ZP_05395295.1 255528511 Clostridium carboxidivorans P7 CcarbDRAFT_1615 ZP_05391615.1 255524662 Clostridium carboxidivorans P7 CcarbDRAFT_1304 ZP_05391304.1 255524347 Clostridium carboxidivorans P7 cooF AAG29808.1 11095245 Carboxydothermus hydrogenoformans fdxN CAA35699.1 46143 Rhodobacter capsulatus Rru_A2264 ABC23064.1 83576513 Rhodospirillum rubrum Rru_A1916 ABC22716.1 83576165 Rhodospirillum rubrum Rru_A2026 ABC22826.1 83576275 Rhodospirillum rubrum cooF AAC45122.1 1498747 Rhodospirillum rubrum fdxN AAA26460.1 152605 Rhodospirillum rubrum Alvin_2884 ADC63789.1 288897953 Allochromatium vinosum DSM 180 Fdx YP_002801146.1 226946073 Azotobacter vinelandii DJ CKL_3790 YP_001397146.1 153956381 Clostridium kluyveri DSM 555 fer1 NP_949965.1 39937689 Rhodopseudomonas palustris CGA009 Fdx CAA12251.1 3724172 Thauera aromatica CHY_2405 YP_361202.1 78044690 Carboxydothermus hydrogenoformans Fer YP_359966.1 78045103 Carboxydothermus hydrogenoformans Fer AAC83945.1 1146198 Bacillus subtilis fdx1 NP_249053.1 15595559 Pseudomonas aeruginosa PA01 yfhL AP_003148.1 89109368 Escherichia coli K-12 CLJU_c00930 ADK13195.1 300433428 Clostridium ljungdahli CLJU_c00010 ADK13115.1 300433348 Clostridium ljungdahli CLJU_c01820 ADK13272.1 300433505 Clostridium ljungdahli CLJU_c17980 ADK14861.1 300435094 Clostridium ljungdahli CLJU_c17970 ADK14860.1 300435093 Clostridium ljungdahli CLJU_c22510 ADK15311.1 300435544 Clostridium ljungdahli CLJU_c26680 ADK15726.1 300435959 Clostridium ljungdahli CLJU_c29400 ADK15988.1 300436221 Clostridium ljungdahli

Ferredoxin oxidoreductase enzymes transfer electrons from ferredoxins or flavodoxins to NAD(P)H. Two enzymes catalyzing the reversible transfer of electrons from reduced ferredoxins to NAD(P)+ are ferredoxin:NAD+ oxidoreductase (EC 1.18.1.3) and ferredoxin:NADP+ oxidoreductase (FNR, EC 1.18.1.2). Ferredoxin:NADP+ oxidoreductase (FNR, EC 1.18.1.2) has a noncovalently bound FAD cofactor that facilitates the reversible transfer of electrons from NADPH to low-potential acceptors such as ferredoxins or flavodoxins (Blaschkowski et al., Eur. J. Biochem. 123:563-569 (1982); Fujii et al., 1977). The Helicobacter pylori FNR, encoded by HP1164 (fqrB), is coupled to the activity of pyruvate:ferredoxin oxidoreductase (PFOR) resulting in the pyruvate-dependent production of NADPH (St et al. 2007). An analogous enzyme is found in Campylobacter jejuni (St Maurice et al., J. Bacteriol. 189:4764-4773 (2007)). A ferredoxin:NADP+ oxidoreductase enzyme is encoded in the E. coli genome by fpr (Bianchi et al. 1993). Ferredoxin:NAD+ oxidoreductase utilizes reduced ferredoxin to generate NADH from NAD+. In several organisms, including E. coli, this enzyme is a component of multifunctional dioxygenase enzyme complexes. The ferredoxin:NAD+ oxidoreductase of E. coli, encoded by hcaD, is a component of the 3-phenylproppionate dioxygenase system involved in involved in aromatic acid utilization (Diaz et al. 1998). NADH:ferredoxin reductase activity was detected in cell extracts of Hydrogenobacter thermophilus, although a gene with this activity has not yet been indicated (Yoon et al. 2006). Additional ferredoxin:NAD(P)+ oxidoreductases have been annotated in Clostridium carboxydivorans P7. The NADH-dependent reduced ferredoxin: NADP oxidoreductase of C. kluyveri, encoded by nfnAB, catalyzes the concomitant reduction of ferredoxin and NAD+ with two equivalents of NADPH (Wang et al, J Bacteriol 192: 5115-5123 (2010)). Finally, the energy-conserving membrane-associated Rnf-type proteins (Seedorf et al, PNAS 105:2128-2133 (2008); and Herrmann, J Bacteriol 190:784-791 (2008)) provide a means to generate NADH or NADPH from reduced ferredoxin.

Protein GenBank ID GI Number Organism fqrB NP_207955.1 15645778 Helicobacter pylori fqrB YP_001482096.1 157414840 Campylobacter jejuni RPA3954 CAE29395.1 39650872 Rhodopseudomonas palustris Fpr BAH29712.1 225320633 Hydrogenobacter thermophilus yumC NP_391091.2 255767736 Bacillus subtilis Fpr P28861.4 399486 Escherichia coli hcaD AAC75595.1 1788892 Escherichia coli LOC100282643 NP_001149023.1 226497434 Zea mays NfnA YP_001393861.1 153953096 Clostridium kluyveri NfnB YP_001393862.1 153953097 Clostridium kluyveri CcarbDRAFT_2639 ZP_05392639.1 255525707 Clostridium carboxidivorans P7 CcarbDRAFT_2638 ZP_05392638.1 255525706 Clostridium carboxidivorans P7 CcarbDRAFT_2636 ZP_05392636.1 255525704 Clostridium carboxidivorans P7 CcarbDRAFT_5060 ZP_05395060.1 255528241 Clostridium carboxidivorans P7 CcarbDRAFT_2450 ZP_05392450.1 255525514 Clostridium carboxidivorans P7 CcarbDRAFT_1084 ZP_05391084.1 255524124 Clostridium carboxidivorans P7 RnfC EDK33306.1 146346770 Clostridium kluyveri RnfD EDK33307.1 146346771 Clostridium kluyveri RnfG EDK33308.1 146346772 Clostridium kluyveri RnfE EDK33309.1 146346773 Clostridium kluyveri RnfA EDK33310.1 146346774 Clostridium kluyveri RnfB EDK33311.1 146346775 Clostridium kluyveri CLJU_c11410 (RnfB) ADK14209.1 300434442 Clostridium ljungdahlii CLJU_c11400 (RnfA) ADK14208.1 300434441 Clostridium ljungdahlii CLJU_c11390 (RnfE) ADK14207.1 300434440 Clostridium ljungdahlii CLJU_c11380 (RnfG) ADK14206.1 300434439 Clostridium ljungdahlii CLJU_c11370 (RnfD) ADK14205.1 300434438 Clostridium ljungdahlii CLJU_c11360 (RnfC) ADK14204.1 300434437 Clostridium ljungdahlii MOTH_1518 (NfnA) YP_430370.1 83590361 Moorella thermoacetica MOTH_1517 (NfnB) YP_430369.1 83590360 Moorella thermoacetica CHY_1992 (NfnA) YP_360811.1 78045020 Carboxydothermus hydrogenoformans CHY_1993 (NfnB) YP_360812.1 78044266 Carboxydothermus hydrogenoformans CLJU_c37220 (NfnAB) YP_003781850.1 300856866 Clostridium ljungdahlii

FIG. 10, Step I—Formate Dehydrogenase

Formate dehydrogenase (FDH) catalyzes the reversible transfer of electrons from formate to an acceptor. Enzymes with FDH activity utilize various electron carriers such as, for example, NADH (EC 1.2.1.2), NADPH (EC 1.2.1.43), quinols (EC 1.1.5.6), cytochromes (EC 1.2.2.3) and hydrogenases (EC 1.1.99.33). FDH enzymes have been characterized from Moorella thermoacetica (Andreesen and Ljungdahl, J Bacteriol 116:867-873 (1973); Li et al., J Bacteriol 92:405-412 (1966); Yamamoto et al., J Biol Chem. 258:1826-1832 (1983). The loci, Moth 2312 is responsible for encoding the alpha subunit of formate dehydrogenase while the beta subunit is encoded by Moth_2314 (Pierce et al., Environ Microbiol (2008)). Another set of genes encoding formate dehydrogenase activity with a propensity for CO₂ reduction is encoded by Sfum_2703 through Sfum_2706 in Syntrophobacter fumaroxidans (de Bok et al., Eur J Biochem. 270:2476-2485 (2003)); Reda et al., PNAS 105:10654-10658 (2008)). A similar set of genes presumed to carry out the same function are encoded by CHY_0731, CHY_0732, and CHY_0733 in C. hydrogenoformans (Wu et al., PLoS Genet 1:e65 (2005)). Formate dehydrogenases are also found many additional organisms including C. carboxidivorans P7, Bacillus methanolicus, Burkholderia stabilis, Moorella thermoacetica ATCC 39073, Candida boidinii, Candida methylica, and Saccharomyces cerevisiae S288c. The soluble formate dehydrogenase from Ralstonia eutropha reduces NAD⁺ (fdsG, -B, -A, -C, -D) (Oh and Bowien, 1998)

Protein GenBank ID GI Number Organism Moth_2312 YP_431142 148283121 Moorella thermoacetica Moth_2314 YP_431144 83591135 Moorella thermoacetica Sfum_2703 YP_846816.1 116750129 Syntrophobacter fumaroxidans Sfum_2704 YP_846817.1 116750130 Syntrophobacter fumaroxidans Sfum_2705 YP_846818.1 116750131 Syntrophobacter fumaroxidans Sfum_2706 YP_846819.1 116750132 Syntrophobacter fumaroxidans CHY_0731 YP_359585.1 78044572 Carboxydothermus hydrogenoformans CHY_0732 YP_359586.1 78044500 Carboxydothermus hydrogenoformans CHY_0733 YP_359587.1 78044647 Carboxydothermus hydrogenoformans CcarbDRAFT_0901 ZP_05390901.1 255523938 Clostridium carboxidivorans P7 CcarbDRAFT_4380 ZP_05394380.1 255527512 Clostridium carboxidivorans P7 fdhA, MGA3_06625 EIJ82879.1 387590560 Bacillus methanolicus MGA3 fdhA, PB1_11719 ZP_10131761.1 387929084 Bacillus methanolicus PB1 fdhD, MGA3_06630 EIJ82880.1 387590561 Bacillus methanolicus MGA3 fdhD, PB1_11724 ZP_10131762.1 387929085 Bacillus methanolicus PB1 fdh ACF35003. 194220249 Burkholderia stabilis FDH1 AAC49766.1 2276465 Candida boidinii fdh CAA57036.1 1181204 Candida methylica FDH2 P0CF35.1 294956522 Saccharomyces cerevisiae S288c FDH1 NP_015033.1 6324964 Saccharomyces cerevisiae S288c fdsG YP_725156.1 113866667 Ralstonia eutropha fdsB YP_725157.1 113866668 Ralstonia eutropha fdsA YP_725158.1 113866669 Ralstonia eutropha fdsC YP_725159.1 113866670 Ralstonia eutropha fdsD YP_725160.1 113866671 Ralstonia eutropha

FIG. 10, Step J—Methanol Dehydrogenase

NAD+ dependent methanol dehydrogenase enzymes (EC 1.1.1.244) catalyze the conversion of methanol and NAD+ to formaldehyde and NADH. An enzyme with this activity was first characterized in Bacillus methanolicus (Heggeset, et al., Applied and Environmental Microbiology, 78(15):5170-5181(2012)). This enzyme is zinc and magnesium dependent, and activity of the enzyme is enhanced by the activating enzyme encoded by act (Kloosterman et al, J Biol Chem 277:34785-92 (2002)). The act is a Nudix hydrolase. Several of these candidates have been identified and shown to have activity on methanol. Additional NAD(P)+ dependent enzymes can be identified by sequence homology. Methanol dehydrogenase enzymes utilizing different electron acceptors are also known in the art. Examples include cytochrome dependent enzymes such as mxaIF of the methylotroph Methylobacterium extorquens (Nunn et al, Nucl Acid Res 16:7722 (1988)). Methanol dehydrogenase enzymes of methanotrophs such as Methylococcus capsulatis function in a complex with methane monooxygenase (MMO) (Myronova et al, Biochem 45:11905-14 (2006)). Methanol can also be oxidized to formaldehyde by alcohol oxidase enzymes such as methanol oxidase (EC 1.1.3.13) of Candida boidinii (Sakai et al, Gene 114: 67-73 (1992)).

Protein GenBank ID GI Number Organism mdh, MGA3_17392 EIJ77596.1 387585261 Bacillus methanolicus MGA3 mdh2, MGA3_07340 EIJ83020.1 387590701 Bacillus methanolicus MGA3 mdh3, MGA3_10725 EIJ80770.1 387588449 Bacillus methanolicus MGA3 act, MGA3_09170 EIJ83380.1 387591061 Bacillus methanolicus MGA3 mdh, PB1_17533 ZP_10132907.1 387930234 Bacillus methanolicus PB1 mdh1, PB1_14569 ZP_10132325.1 387929648 Bacillus methanolicus PB1 mdh2, PB1_12584 ZP_10131932.1 387929255 Bacillus methanolicus PB1 act, PB1_14394 ZP_10132290.1 387929613 Bacillus methanolicus PB1 BFZC1_05383 ZP_07048751.1 299535429 Lysinibacillus fusiformis BFZC1_20163 ZP_07051637.1 299538354 Lysinibacillus fusiformis Bsph_4187 YP_001699778.1 169829620 Lysinibacillus sphaericus Bsph_1706 YP_001697432.1 169827274 Lysinibacillus sphaericus mdh2 YP_004681552.1 339322658 Cupriavidus necator N-1 nudF1 YP_004684845.1 339325152 Cupriavidus necator N-1 BthaA_010200007655 ZP_05587334.1 257139072 Burkholderia thailandensis E264 BTH_I1076 (MutT/NUDIX YP_441629.1 83721454 Burkholderia thailandensis E264 NTP pyrophosphatase) BalcAV_11743 ZP_10819291.1 402299711 Bacillus alcalophilus ATCC 27647 BalcAV_05251 ZP_10818002.1 402298299 Bacillus alcalophilus ATCC 27647 alcohol dehydrogenase YP_001447544 156976638 Vibrio harveyi ATCC BAA-1116 P3TCK_27679 ZP_01220157.1 90412151 Photobacterium profundum 3TCK alcohol dehydrogenase YP_694908 110799824 Clostridium perfringens ATCC 13124 adhB NP_717107 24373064 Shewanella oneidensis MR-1 alcohol dehydrogenase YP_237055 66047214 Pseudomonas syringae pv. syringae B728a alcohol dehydrogenase YP_359772 78043360 Carboxydothemus hydrogenoformans Z-2901 alcohol dehydrogenase YP_003990729 312112413 Geobacillus sp. Y4.1MC1 PpeoK3_010100018471 ZP_10241531.1 390456003 Paenibacillus peoriae KCTC 3763 OBE_12016 EKC54576 406526935 human gut metagenome alcohol dehydrogenase YP_001343716 152978087 Actinobacillus succinogenes 130Z dhaT AAC45651 2393887 Clostridium pasteurianum DSM 525 alcohol dehydrogenase NP_561852 18309918 Clostridium perfringens str. 13 BAZO_10081 ZP_11313277.1 410459529 Bacillus azotoformans LMG 9581 alcohol dehydrogenase YP_007491369 452211255 Methanosarcina mazei Tuc01 alcohol dehydrogenase YP_004860127 347752562 Bacillus coagulans 36D1 alcohol dehydrogenase YP_002138168 197117741 Geobacter bemidjiensis Bem DesmeDRAFT_1354 ZP_08977641.1 354558386 Desulfitobacterium metallireducens DSM 15288 alcohol dehydrogenase YP_001337153 152972007 Klebsiella pneumoniae subsp. pneumoniae MGH 78578 alcohol dehydrogenase YP_001113612 134300116 Desulfotomaculum reducens MI-1 alcohol dehydrogenase YP_001663549 167040564 Thermoanaerobacter sp. X514 ACINNAV82_2382 ZP_16224338.1 421788018 Acinetobacter baumannii Naval-82 alcohol dehydrogenase YP_005052855 374301216 Desulfovibrio africanus str. Walvis Bay alcohol dehydrogenase AGF87161 451936849 uncultured organism DesfrDRAFT_3929 ZP_07335453.1 303249216 Desulfovibrio fructosovorans JJ alcohol dehydrogenase NP_617528 20091453 Methanosarcina acetivorans C2A alcohol dehydrogenase NP_343875.1 15899270 Sulfolobus solfataricus P-2 adh4 YP_006863258 408405275 Nitrososphaera gargensis Ga9.2 Ta0841 NP_394301.1 16081897 Thermoplasma acidophilum PTO1151 YP_023929.1 48478223 Picrophilus torridus DSM9790 alcohol dehydrogenase ZP_10129817.1 387927138 Bacillus methanolicus PB-1 cgR_2695 YP_001139613.1 145296792 Corynebacterium glutamicum R alcohol dehydrogenase YP_004758576.1 340793113 Corynebacterium variabile HMPREF1015_01790 ZP_09352758.1 365156443 Bacillus smithii ADH1 NP_014555.1 6324486 Saccharomyces cerevisiae NADH-dependent butanol YP_001126968.1 138896515 Geobacillus themodenitrificans dehydrogenase A NG80-2 alcohol dehydrogenase WP_007139094.1 494231392 Flavobacterium frigoris methanol dehydrogenase WP_003897664.1 489994607 Mycobacterium smegmatis ADH1B NP_000659.2 34577061 Homo sapiens PMI01_01199 ZP_10750164.1 399072070 Caulobacter sp. AP07 YiaY YP_026233.1 49176377 Escherichia coli MCA0299 YP_112833.1 53802410 Methylococcus capsulatis MCA0782 YP_113284.1 53804880 Methylococcus capsulatis mxaI YP_002965443.1 240140963 Methylobacterium extorquens mxaF YP_002965446.1 240140966 Methylobacterium extorquens AOD1 AAA34321.1 170820 Candida boidinii hypothetical protein EDA87976.1 142827286 Marine metagenome GOS_1920437 JCVI_SCAF_1096627185304 alcohol dehydrogenase CAA80989.1 580823 Geobacillus stearothermophilus

An in vivo assay was developed to determine the activity of methanol dehydrogenases. This assay relies on the detection of formaldehyde (HCHO), thus measuring the forward activity of the enzyme (oxidation of methanol). To this end, a strain comprising a BDOP and lacking frmA, frmB, frmR was created using Lamba Red recombinase technology (Datsenko and Wanner, Proc. Natl. Acad. Sci. USA, 6 97(12): 6640-5 (2000). Plasmids expressing methanol dehydrogenases were transformed into the strain, then grown to saturation in LB medium+antibiotic at 37° C. with shaking. Transformation of the strain with an empty vector served as a negative control. Cultures were adjusted by O.D. and then diluted 1:10 into M9 medium+0.5% glucose+antibiotic and cultured at 37° C. with shaking for 6-8 hours until late log phase. Methanol was added to 2% v/v and the cultures were further incubated for 30 min. with shaking at 37° C. Cultures were spun down and the supernatant was assayed for formaldehyde produced using DETECTX Formaldehyde Detection kit (Arbor Assays; Ann Arbor, Mich.) according to manufacturer's instructions. The frmA, frmB, frmR deletions resulted in the native formaldehyde utilization pathway to be deleted, which enables the formation of formaldehyde that can be used to detect methanol dehydrogenase activity in the NNOMO.

The activity of several enzymes was measured using the assay described above. The results of four independent experiments are provided in Table 5 below.

TABLE 5 Results of in vivo assays showing formaldehyde (HCHO) production by various NNOMO comprising a plasmid expressing a methanol dehydrogenase. Accession Accession Accession number HCHO Accession number HCHO number HCHO number HCHO Experiment 1 (μM) Experiment 2 (μM) Experiment 3 (μM) Experiment 4 (μM) EIJ77596.1 >50 EIJ77596.1 >50 EIJ77596.1 >50 EIJ77596.1 >20 EIJ83020.1 >20 NP_00659.2 >50 NP_561852 >50 ZP_11313277.1 >50 EIJ80770.1 >50 YP_004758576.1 >20 YP_002138168 >50 YP_001113612 >50 ZP_10132907.1 >20 ZP_09352758.1 >50 YP_026233.1 >50 YP_001447544 >20 ZP_10132325.1 >20 ZP_10129817.1 >20 YP_001447544 >50 AGF87161 >50 ZP_10131932.1 >50 YP_001139613.1 >20 Metalibrary >50 EDA87976.1 >20 ZP_07048751.1 >50 NP_014555.1 >10 YP_359772 >50 Empty vector −0.8 YP_001699778.1 >50 WP_007139094.1 >10 ZP_01220157.1 >50 YP_004681552.1 >10 NP_343875.1 >1 ZP_07335453.1 >20 ZP_10819291.1 <1 YP_006863258 >1 YP_001337153 >20 Empty vector 2.33 NP_394301.1 >1 YP_694908 >20 ZP_10750164.1 >1 NP_717107 >20 YP_023929.1 >1 AAC45651 >10 ZP_08977641.1 <1 ZP_11313277.1 >10 ZP_10117398.1 <1 ZP_16224338.1 >10 YP_004108045.1 <1 YP_001113612 >10 ZP_09753449.1 <1 YP_004860127 >10 Empty vector 0.17 YP_003310546 >10 YP_001343716 >10 NP_717107 >10 YP_002434746 >10 Empty vector 0.11

FIG. 10, Step K—Spontaneous or Formaldehyde Activating Enzyme

The conversion of formaldehyde and THF to methylenetetrahydrofolate can occur spontaneously. It is also possible that the rate of this reaction can be enhanced by a formaldehyde activating enzyme. A formaldehyde activating enzyme (Fae) has been identified in Methylobacterium extorquens AM1 which catalyzes the condensation of formaldehyde and tetrahydromethanopterin to methylene tetrahydromethanopterin (Vorholt, et al., J. Bacteriol., 182(23), 6645-6650 (2000)). It is possible that a similar enzyme exists or can be engineered to catalyze the condensation of formaldehyde and tetrahydrofolate to methylenetetrahydrofolate. Homologs exist in several organisms including Xanthobacter autotrophicus Py2 and Hyphomicrobium denitrificans ATCC 51888.

Protein GenBank ID GI Number Organism MexAM1_META1p1766 Q9FA38.3 17366061 Methylobacterium extorquens AM1 Xaut_0032 YP_001414948.1 154243990 Xanthobacter autotrophicus Py2 Hden_1474 YP_003755607.1 300022996 Hyphomicrobium denitrificans ATCC 51888

FIG. 10, Step L—Formaldehyde Dehydrogenase

Oxidation of formaldehyde to formate is catalyzed by formaldehyde dehydrogenase. An NAD+ dependent formaldehyde dehydrogenase enzyme is encoded by fdhA of Pseudomonas putida (Ito et al, J Bacteriol 176: 2483-2491 (1994)). Additional formaldehyde dehydrogenase enzymes include the NAD+ and glutathione independent formaldehyde dehydrogenase from Hyphomicrobium zavarzinii (Jerome et al, Appl Microbiol Biotechnol 77:779-88 (2007)), the glutathione dependent formaldehyde dehydrogenase of Pichia pastoris (Sunga et al, Gene 330:39-47 (2004)) and the NAD(P)+ dependent formaldehyde dehydrogenase of Methylobacter marinus (Speer et al, FEMS Microbiol Lett, 121(3):349-55 (1994)).

Protein GenBank ID GI Number Organism fdhA P46154.3 1169603 Pseudomonas putida faoA CAC85637.1 19912992 Hyphomicrobium zavarzinii Fld1 CCA39112.1 328352714 Pichia pastoris fdh P47734.2 221222447 Methylobacter marinus

In addition to the formaldehyde dehydrogenase enzymes listed above, alternate enzymes and pathways for converting formaldehyde to formate are known in the art. For example, many organisms employ glutathione-dependent formaldehyde oxidation pathways, in which formaldehyde is converted to formate in three steps via the intermediates S-hydroxymethylglutathione and S-formylglutathione (Vorholt et al, J Bacteriol 182:6645-50 (2000)). The enzymes of this pathway are S-(hydroxymethyl)glutathione synthase (EC 4.4.1.22), glutathione-dependent formaldehyde dehydrogenase (EC 1.1.1.284) and S-formylglutathione hydrolase (EC 3.1.2.12).

FIG. 10, Step M—Spontaneous or S-(Hydroxymethyl)Glutathione Synthase

While conversion of formaldehyde to S-hydroxymethylglutathione can occur spontaneously in the presence of glutathione, it has been shown by Goenrich et al (Goenrich, et al., J Biol Chem 277(5); 3069-72 (2002)) that an enzyme from Paracoccus denitrificans can accelerate this spontaneous condensation reaction. The enzyme catalyzing the conversion of formaldehyde and glutathione was purified and named glutathione-dependent formaldehyde-activating enzyme (Gfa). The gene encoding it, which was named gfa, is located directly upstream of the gene for glutathione-dependent formaldehyde dehydrogenase, which catalyzes the subsequent oxidation of S-hydroxymethylglutathione. Putative proteins with sequence identity to Gfa from P. denitrificans are present also in Rhodobacter sphaeroides, Sinorhizobium meliloti, and Mesorhizobium loti.

Protein GenBank ID GI Number Organism Gfa Q51669.3 38257308 Paracoccus denitrificans Gfa ABP71667.1 145557054 Rhodobacter sphaeroides ATCC 17025 Gfa Q92WX6.1 38257348 Sinorhizobium meliloti 1021 Gfa Q98LU4.2 38257349 Mesorhizobium loti MAFF303099

FIG. 10, Step N—Glutathione-Dependent Formaldehyde Dehydrogenase

Glutathione-dependent formaldehyde dehydrogenase (GS-FDH) belongs to the family of class III alcohol dehydrogenases. Glutathione and formaldehyde combine non-enzymatically to form hydroxymethylglutathione, the true substrate of the GS-FDH catalyzed reaction. The product, S-formylglutathione, is further metabolized to formic acid.

Protein GenBank ID GI Number Organism frmA YP_488650.1 388476464 Escherichia coli K-12 MG1655 SFA1 NP_010113.1 6320033 Saccharomyces cerevisiae S288c flhA AAC44551.1 1002865 Paracoccus denitrificans adhI AAB09774.1 986949 Rhodobacter sphaeroides

FIG. 10, Step O—S-formylglutathione Hydrolase

S-formylglutathione hydrolase is a glutathione thiol esterase found in bacteria, plants and animals. It catalyzes conversion of S-formylglutathione to formate and glutathione. The fghA gene of P. denitrificans is located in the same operon with gfa and flhA, two genes involved in the oxidation of formaldehyde to formate in this organism. In E. coli, FrmB is encoded in an operon with FrmR and FrmA, which are proteins involved in the oxidation of formaldehyde. YeiG of E. coli is a promiscuous serine hydrolase; its highest specific activity is with the substrate S-formylglutathione.

Protein GenBank ID GI Number Organism frmB NP_414889.1 16128340 Escherichia coli K-12 MG1655 yeiG AAC75215.1 1788477 Escherichia coli K-12 MG1655 fghA AAC44554.1 1002868 Paracoccus denitrificans

FIG. 10, Step P—Carbon Monoxide Dehydrogenase (CODH)

CODH is a reversible enzyme that interconverts CO and CO₂ at the expense or gain of electrons. The natural physiological role of the CODH in ACS/CODH complexes is to convert CO₂ to CO for incorporation into acetyl-CoA by acetyl-CoA synthase. Nevertheless, such CODH enzymes are suitable for the extraction of reducing equivalents from CO due to the reversible nature of such enzymes. Expressing such CODH enzymes in the absence of ACS allows them to operate in the direction opposite to their natural physiological role (i.e., CO oxidation).

In M. thermoacetica, C. hydrogenoformans, C. carboxidivorans P7, and several other organisms, additional CODH encoding genes are located outside of the ACS/CODH operons. These enzymes provide a means for extracting electrons (or reducing equivalents) from the conversion of carbon monoxide to carbon dioxide. The M. thermoacetica gene (Genbank Accession Number: YP_430813) is expressed by itself in an operon and is believed to transfer electrons from CO to an external mediator like ferredoxin in a “Ping-pong” reaction. The reduced mediator then couples to other reduced nicolinamide adenine dinucleotide phosphate (NAD(P)H) carriers or ferredoxin-dependent cellular processes (Ragsdale, Annals of the New York Academy of Sciences 1125: 129-136 (2008)). The genes encoding the C. hydrogenoformans CODH-II and CooF, a neighboring protein, were cloned and sequenced (Gonzalez and Robb, FEMS Microbiol Lett. 191:243-247 (2000)). The resulting complex was membrane-bound, although cytoplasmic fractions of CODH-II were shown to catalyze the formation of NADPH suggesting an anabolic role (Svetlitchnyi et al., J Bacteriol. 183:5134-5144 (2001)). The crystal structure of the CODH-II is also available (Dobbek et al., Science 293:1281-1285 (2001)). Similar ACS-free CODH enzymes can be found in a diverse array of organisms including Geobacter metallireducens GS-15, Chlorobium phaeobacteroides DSM 266, Clostridium cellulolyticum H10, Desulfovibrio desulfuricans subsp. desulfuricans str. ATCC 27774, Pelobacter carbinolicus DSM 2380, C. ljungdahli and Campylobacter curvus 525.92.

Protein GenBank ID GI Number Organism CODH (putative) YP_430813 83590804 Moorella thermoacetica CODH-II (CooS-II) YP_358957 78044574 Carboxydothermus hydrogenoformans CooF YP_358958 78045112 Carboxydothermus hydrogenoformans CODH (putative) ZP_05390164.1 255523193 Clostridium carboxidivorans P7 CcarbDRAFT_0341 ZP_05390341.1 255523371 Clostridium carboxidivorans P7 CcarbDRAFT_1756 ZP_05391756.1 255524806 Clostridium carboxidivorans P7 CcarbDRAFT_2944 ZP_05392944.1 255526020 Clostridium carboxidivorans P7 CODH YP_384856.1 78223109 Geobacter metallireducens GS-15 Cpha266_0148 YP_910642.1 119355998 Chlorobium phaeobacteroides DSM (cytochrome c) 266 Cpha266_0149 YP_910643.1 119355999 Chlorobium phaeobacteroides DSM (CODH) 266 Ccel_0438 YP_002504800.1 220927891 Clostridium cellulolyticum H10 Ddes_0382 YP_002478973.1 220903661 Desulfovibrio desulfuricans subsp. (CODH) desulfuricans str. ATCC 27774 Ddes_0381 (CooC) YP_002478972.1 220903660 Desulfovibrio desulfuricans subsp. desulfuricans str. ATCC 27774 Pcar_0057 (CODH) YP_355490.1 7791767 Pelobacter carbinolicus DSM 2380 Pcar_0058 (CooC) YP_355491.1 7791766 Pelobacter carbinolicus DSM 2380 Pcar_0058 (HypA) YP_355492.1 7791765 Pelobacter carbinolicus DSM 2380 CooS (CODH) YP_001407343.1 154175407 Campylobacter curvus 525.92 CLJU_c09110 ADK13979.1 300434212 Clostridium ljungdahli CLJU_c09100 ADK13978.1 300434211 Clostridium ljungdahli CLJU_c09090 ADK13977.1 300434210 Clostridium ljungdahli

Example III Methods for Formaldehyde Fixation

Provided herein are exemplary pathways, which utilize formaldehyde produced from the oxidation of methanol (see, e.g., FIG. 1, step A, or FIG. 10, step J) or from formate assimilation pathways described in Example I (see, e.g., FIG. 1) in the formation of intermediates of certain central metabolic pathways that can be used for the production of compounds disclosed herein.

One exemplary pathway that can utilize formaldehyde produced from the oxidation of methanol is shown in FIG. 1, which involves condensation of formaldehyde and D-ribulose-5-phosphate to form hexulose-6-phosphate (h6p) by hexulose-6-phosphate synthase (FIG. 1, step B). The enzyme can use Mg²⁺ or Mn²⁺ for maximal activity, although other metal ions are useful, and even non-metal-ion-dependent mechanisms are contemplated. H6p is converted into fructose-6-phosphate by 6-phospho-3-hexuloisomerase (FIG. 1, step C).

Another exemplary pathway that involves the detoxification and assimilation of formaldehyde produced from the oxidation of methanol is shown in FIG. 1 and proceeds through dihydroxyacetone. Dihydroxyacetone synthase is a special transketolase that first transfers a glycoaldehyde group from xylulose-5-phosphate to formaldehyde, resulting in the formation of dihydroxyacetone (DHA) and glyceraldehyde-3-phosphate (G3P), which is an intermediate in glycolysis (FIG. 1). The DHA obtained from DHA synthase can be further phosphorylated to form DHA phosphate and assimilated into glycolysis and several other pathways (FIG. 1). Alternatively, or in addition, a fructose-6-phosphate aldolase can be used to catalyze the conversion of DHA and G3P to fructose-6-phosphate (FIG. 1, step Z).

FIG. 1, Steps B and C—Hexulose-6-Phosphate Synthase (Step B) and 6-Phospho-3-Hexuloisomerase (Step C)

Both of the hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase enzymes are found in several organisms, including methanotrops and methylotrophs where they have been purified (Kato et al., 2006, BioSci Biotechnol Biochem. 70(1):10-21. In addition, these enzymes have been reported in heterotrophs such as Bacillus subtilis also where they are reported to be involved in formaldehyde detoxification (Mitsui et al., 2003, AEM 69(10):6128-32, Yasueda et al., 1999. J Bac 181(23):7154-60. Genes for these two enzymes from the methylotrophic bacterium Mycobacterium gastri MB19 have been fused and E. coli strains harboring the hps-phi construct showed more efficient utilization of formaldehyde (Orita et al., 2007, Appl Microbiol Biotechnol. 76:439-445). In some organisms, these two enzymes naturally exist as a fused version that is bifunctional.

Exemplary candidate genes for hexulose-6-phosphate synthase are:

Protein GenBank ID GI number Organism Hps AAR39392.1 40074227 Bacillus methanolicus MGA3 Hps EIJ81375.1 387589055 Bacillus methanolicus PB1 RmpA BAA83096.1 5706381 Methylomonas aminofaciens RmpA BAA90546.1 6899861 Mycobacterium gastri YckG BAA08980.1 1805418 Bacillus subtilis Hps YP_544362.1 91774606 Methylobacillus flagellatus Hps YP_545763.1 91776007 Methylobacillus flagellatus Hps AAG29505.1 11093955 Aminomonas aminovorus SgbH YP_004038706.1 313200048 Methylovorus sp. MP688 Hps YP_003050044.1 253997981 Methylovorus glucosetrophus SIP3-4 Hps YP_003990382.1 312112066 Geobacillus sp. Y4.1MC1 Hps gb|AAR91478.1 40795504 Geobacillus sp. M10EXG Hps YP_007402409.1 448238351 Geobacillus sp. GHH01

Exemplary gene candidates for 6-phospho-3-hexuloisomerase are:

Protein GenBank ID GI number Organism Phi AAR39393.1 40074228 Bacillus methanolicus MGA3 Phi EIJ81376.1 387589056 Bacillus methanolicus PB1 Phi BAA83098.1 5706383 Methylomonas aminofaciens RmpB BAA90545.1 6899860 Mycobacterium gastri Phi YP_545762.1 91776006 Methylobacillus flagellatus KT Phi YP_003051269.1 253999206 Methylovorus glucosetrophus SIP3-4 Phi YP_003990383.1 312112067 Geobacillus sp. Y4.1MC1 Phi YP_007402408.1 448238350 Geobacillus sp. GHH01

Candidates for enzymes where both of these functions have been fused into a single open reading frame include the following.

Protein GenBank ID GI number Organism PH1938 NP_143767.1 14591680 Pyrococcus horikoshii OT3 PF0220 NP_577949.1 18976592 Pyrococcus furiosus TK0475 YP_182888.1 57640410 Thermococcus kodakaraensis PAB1222 NP_127388.1 14521911 Pyrococcus abyssi MCA2738 YP_115138.1 53803128 Methylococcus capsulatas Metal_3152 EIC30826.1 380884949 Methylomicrobium album BG8

FIG. 1, Step D—Dihydroxyacetone Synthase

The dihydroxyacetone synthase enzyme in Candida boidinii uses thiamine pyrophosphate and Mg²⁺ as cofactors and is localized in the peroxisome. The enzyme from the methanol-growing carboxydobacterium, Mycobacter sp. strain JC1 DSM 3803, was also found to have DHA synthase and kinase activities (Ro et al., 1997, JBac 179(19):6041-7). DHA synthase from this organism also has similar cofactor requirements as the enzyme from C. boidinii. The K_(m)s for formaldehyde and xylulose 5-phosphate were reported to be 1.86 mM and 33.3 microM, respectively. Several other mycobacteria, excluding only Mycobacterium tuberculosis, can use methanol as the sole source of carbon and energy and are reported to use dihydroxyacetone synthase (Part et al., 2003, JBac 185(1):142-7.

Protein GenBank ID GI number Organism DAS1 AAC83349.1 3978466 Candida boidinii HPODL_2613 EFW95760.1 320581540 Ogataea parapolymorpha DL-1 (Hansenula polymorpha DL-1) AAG12171.2 18497328 Mycobacter sp. strain JC1 DSM 3803 FIG. 1, Step Z—Fructose-6-phosphate aldolase

Fructose-6-phosphate aldolase (F6P aldolase) can catalyze the combination of dihydroxyacetone (DHA) and glyceraldehyde-3-phosphate (G3P) to form fructose-6-phosphate. This activity was recently discovered in E. coli and the corresponding gene candidate has been termed fsa (Schurmann and Sprenger, J. Biol. Chem., 2001, 276(14), 11055-11061). The enzyme has narrow substrate specificity and cannot utilize fructose, fructose 1-phosphate, fructose 1,6-bisphosphate, or dihydroxyacetone phosphate. It can however use hydroxybutanone and acetol instead of DHA. The purified enzyme displayed a V_(max) of 7 units/mg of protein for fructose 6-phosphate cleavage (at 30 degrees C., pH 8.5 in 50 mm glycylglycine buffer). For the aldolization reaction a V_(max) of 45 units/mg of protein was found; K_(m) values for the substrates were 9 mM for fructose 6-phosphate, 35 mM for dihydroxyacetone, and 0.8 mM for glyceraldehyde 3-phosphate. The enzyme prefers the aldol formation over the cleavage reaction.

The selectivity of the E. coli enzyme towards DHA can be improved by introducing point mutations. For example, the mutation A129S improved reactivity towards DHA by over 17 fold in terms of K_(cat)/K_(m) (Gutierrez et al., Chem Commun (Camb), 2011, 47(20), 5762-5764). The same mutation reduced the catalytic efficiency on hydroxyacetone by more than 3 fold and reduced the affinity for glycoaldehyde by more than 3 fold compared to that of the wild type enzyme (Castillo et al., Advanced Synthesis & Catalysis, 352(6), 1039-1046). Genes similar to fsa have been found in other genomes by sequence homology. Some exemplary gene candidates have been listed below.

Protein Gene accession number GI number Organism fsa AAC73912.2 87081788 Escherichia coli K12 talC AAC76928.1 1790382 Escherichia coli K12 fsa WP_017209835.1 515777235 Clostridium beijerinickii DR_1337 AAF10909.1 6459090 Deinococcus radiodurans R1 talC NP_213080.1 15605703 Aquifex aeolicus VF5 MJ_0960 NP_247955.1 15669150 Methanocaldococcus janaschii mipB NP_993370.2 161511381 Yersinia pestis

As described below, there is an energetic advantage to using F6P aldolase in the DHA pathway.

The assimilation of formaldehyde formed by the oxidation of methanol can proceed either via the dihydroxyacetone (DHA) pathway (step D, FIG. 1) or the Ribulose monophosphate (RuMP) pathway (steps B and C, FIG. 1). In the RuMP pathway, formaldehyde combines with ribulose-5-phosphate to form F6P. F6P is then either metabolized via glycolysis or used for regeneration of ribulose-5-phosphate to enable further formaldehyde assimilation. Notably, ATP hydrolysis is not required to form F6P from formaldehyde and ribulose-5-phosphate via the RuMP pathway.

In contrast, in the DHA pathway, formaldehyde combines with xylulose-5-phosphate (X5P) to form dihydroxyacetone (DHA) and glyceraldehyde-3-phosphate (G3P). Some of the DHA and G3P must be metabolized to F6P to enable regeneration of xylulose-5-phosphate. In the standard DHA pathway, DHA and G3P are converted to F6P by three enzymes: DHA kinase, fructose bisphosphate aldolase, and fructose bisphosphatase. The net conversion of DHA and G3P to F6P requires ATP hydrolysis as described below. First, DHA is phosphorylated to form DHA phosphate (DHAP) by DHA kinase at the expense of an ATP. DHAP and G3P are then combined by fructose bisphosphate aldolase to form fructose-1,6-diphosphate (FDP). FDP is converted to F6P by fructose bisphosphatase, thus wasting a high energy phosphate bond.

A more ATP efficient sequence of reactions is enabled if DHA synthase functions in combination with F6P aldolase as opposed to in combination with DHA kinase, fructose bisphosphate aldolase, and fructose bisphosphatase. F6P aldolase enables direct conversion of DHA and G3P to F6P, bypassing the need for ATP hydrolysis. Overall, DHA synthase when combined with F6P aldolase is identical in energy demand to the RuMP pathway. Both of these formaldehyde assimilation options (i.e., RuMP pathway, DHA synthase+F6P aldolase) are superior to DHA synthase combined with DHA kinase, fructose bisphosphate aldolase, and fructose bisphosphatase in terms of ATP demand.

Example IV Production of Fatty Alcohols and Fatty Aldehydes by MI-FAE Cycle, MD-FAE Cycle and Acyl-CoA Termination Pathways

Encoding nucleic acids and species that can be used as sources for conferring fatty alcohol and fatty aldehyde production capability onto a host microbial organism are exemplified further below.

Multienzyme Complexes

In one exemplary embodiment, the genes fadA and fadB encode a multienzyme complex that exhibits three constituent activities of the malonyl-CoA independent FAS pathway, namely, ketoacyl-CoA thiolase, 3-hydroxyacyl-CoA dehydrogenase, and enoyl-CoA hydratase activities (Nakahigashi, K. and H. Inokuchi, Nucleic Acids Research 18:4937 (1990); Yang et al., Journal of Bacteriology 173:7405-7406 (1991); Yang et al, Journal of Biological Chemistry 265:10424-10429 (1990); Yang et al., Biochemistry 30:6788-6795 (1990)). The fadI and fadJ genes encode similar activities which can substitute for the above malonyl-CoA independent FAS conferring genes fadA and fadB. The acyl-CoA dehydrogenase of E. coli is encoded by fadE (Campbell et al, J Bacteriol 184: 3759-64)). This enzyme catalyzes the rate-limiting step of beta-oxidation (O'Brien et al, J Bacteriol 132:532-40 (1977)). The nucleic acid sequences for each of the above fad genes are well known in the art and can be accessed in the public databases such as Genbank using the following accession numbers.

Protein GenBank ID GI Number Organism fadA YP_026272.1 49176430 Escherichia coli fadB NP_418288.1 16131692 Escherichia coli fadI NP_416844.1 16130275 Escherichia coli fadJ NP_416843.1 16130274 Escherichia coli fadR NP_415705.1 16129150 Escherichia coli fadE AAC73325.2 87081702 Escherichia coli

Step A. Thiolase

Thiolase enzymes, also know as beta-keto thiolase, acyl-CoA C-acetyltransferase, acyl-CoA:acetyl-CoA C-acyltransferase, 3-oxoacyl-CoA thiolase, 3-ketoacyl-CoA thiolase, beta-ketoacyl-CoA thiolase, and acyl-CoA thiolase, that are suitable for fatty alcohol, fatty aldehyde or fatty acid production are described herein (FIGS. 2A and 7A). Exemplary acetoacetyl-CoA thiolase enzymes include the gene products of atoB and homolog yqeF from E. coli (Martin et al., Nat. Biotechnol 21:796-802 (2003)), thlA and thlB from C. acetobutylicum (Hanai et al., Appl Environ Microbiol 73:7814-7818 (2007); Winzer et al., J. Mol. Microbiol Biotechnol 2:531-541 (2000)), and ERG10 from S. cerevisiae (Hiser et al., J. Biol. Chem. 269:31383-31389 (1994)). A degradative thiolase of S. cerevisiae is encoded by POT1. Another candidate thiolase is the phaA gene product of R. eutropha (Jenkins et al, Journal of Bacteriology 169:42-52 (1987)). The acetoacetyl-CoA thiolase from Zoogloea ramigera is irreversible in the biosynthetic direction and a crystal structure is available (Merilainen et al, Biochem 48: 11011-25 (2009)). Accession numbers for these thiolases and homologs are included in the table below.

Protein GenBank ID GI Number Organism atoB NP_416728 16130161 Escherichia coli yqeF NP_417321.2 90111494 Escherichia coli thlA NP_349476.1 15896127 Clostridium acetobutylicum thlB NP_149242.1 15004782 Clostridium acetobutylicum ERG10 NP_015297 6325229 Saccharomyces cerevisiae POT1 NP_012106.1 6322031 Saccharomyces cerevisiae phaA YP_725941 113867452 Ralstonia eutropha phbA P07097.4 135759 Zoogloea ramigera h16_A1713 YP_726205.1 113867716 Ralstonia eutropha pcaF YP_728366.1 116694155 Ralstonia eutropha h16_B1369 YP_840888.1 116695312 Ralstonia eutropha h16_A0170 YP_724690.1 113866201 Ralstonia eutropha h16_A0462 YP_724980.1 113866491 Ralstonia eutropha h16_A1528 YP_726028.1 113867539 Ralstonia eutropha h16_B0381 YP_728545.1 116694334 Ralstonia eutropha h16_B0662 YP_728824.1 116694613 Ralstonia eutropha h16_B0759 YP_728921.1 116694710 Ralstonia eutropha h16_B0668 YP_728830.1 116694619 Ralstonia eutropha h16_A1720 YP_726212.1 113867723 Ralstonia eutropha h16_A1887 YP_726356.1 113867867 Ralstonia eutropha bktB YP_002005382.1 194289475 Cupriavidus taiwanensis Rmet_1362 YP_583514.1 94310304 Ralstonia metallidurans Bphy_0975 YP_001857210.1 186475740 Burkholderia phymatum

Many thiolase enzymes catalyze the formation of longer-chain acyl-CoA products. Exemplary thiolases include, for example, 3-oxoadipyl-CoA thiolase (EC 2.3.1.174) and acyl-CoA thiolase (EC 2.3.1.16). 3-Oxoadipyl-CoA thiolase converts succinyl-CoA and acetyl-CoA to 3-oxoadipyl-CoA, and is a key enzyme of the beta-ketoadipate pathway for aromatic compound degradation. The enzyme is widespread in soil bacteria and fungi including Pseudomonas putida (Harwood et al., J Bacteriol. 176:6479-6488 (1994)) and Acinetobacter calcoaceticus (Doten et al., J Bacteriol. 169:3168-3174 (1987)). The gene products encoded by pcaF in Pseudomonas strain B13 (Kaschabek et al., J Bacteriol. 184:207-215 (2002)), phaD in Pseudomonas putida U (Olivera et al., Proc. Natl. Acad. Sci U.S.A 95:6419-6424 (1998)), paaE in Pseudomonas fluorescens ST (Di et al., Arch. Microbiol 188:117-125 (2007)), and paaJ from E. coli (Nogales et al., Microbiology 153:357-365 (2007)) also catalyze this transformation. Several beta-ketothiolases exhibit significant and selective activities in the oxoadipyl-CoA forming direction including bkt from Pseudomonas putida, pcaF and bkt from Pseudomonas aeruginosa P A01, bkt from Burkholderia ambifaria AMMD, paaJ from E. coli, and phaD from P. putida. Two gene products of Ralstonia eutropha (formerly known as Alcaligenes eutrophus), encoded by genes bktB and bktC, catalyze the formation of 3-oxopimeloyl-CoA (Slater et al., J. Bacteriol. 180:1979-1987 (1998); Haywood et al., FEMS Microbiology Letters 52:91-96 (1988)). The sequence of the BktB protein is known; however, the sequence of the BktC protein has not been reported. BktB is also active on substrates of length C6 and C8 (Machado et al, Met Eng in press (2012)). The pim operon of Rhodopseudomonas palustris also encodes a beta-ketothiolase, encoded by pimB, predicted to catalyze this transformation in the degradative direction during benzoyl-CoA degradation (Harrison et al., Microbiology 151:727-736 (2005)). A beta-ketothiolase enzyme candidate in S. aciditrophicus was identified by sequence homology to bktB (43% identity, evalue=1e-93).

GenBank Gene name GI# Accession # Organism paaJ 16129358 NP_415915.1 Escherichia coli pcaF 17736947 AAL02407 Pseudomonas knackmussii (B13) phaD 3253200 AAC24332.1 Pseudomonas putida pcaF 506695 AAA85138.1 Pseudomonas putida pcaF 141777 AAC37148.1 Acinetobacter calcoaceticus paaE 106636097 ABF82237.1 Pseudomonas fluorescens bkt 115360515 YP_777652.1 Burkholderia ambifaria AMMD bkt 9949744 AAG06977.1 Pseudomonas aeruginosa PAO1 pcaF 9946065 AAG03617.1 Pseudomonas aeruginosa PAO1 bktB YP_725948 11386745 Ralstonia eutropha pimB CAE29156 39650633 Rhodopseudomonas palustris syn_02642 YP_462685.1 85860483 Syntrophus aciditrophicus

Acyl-CoA thiolase (EC 2.3.1.16) enzymes involved in the beta-oxidation cycle of fatty acid degradation exhibit activity on a broad range of acyl-CoA substrates of varying chain length. Exemplary acyl-CoA thiolases are found in Arabidopsis thaliana (Cruz et al, Plant Physiol 135:85-94 (2004)), Homo sapiens (Mannaerts et al, Cell Biochem Biphys 32:73-87 (2000)), Helianthus annuus (Schiedel et al, Prot Expr Purif 33:25-33 (2004)). The chain length specificity of thiolase enzymes can be assayed by methods well known in the art (Wrensford et al, Anal Biochem 192:49-54 (1991)). A peroxisomal thiolase found in rat liver catalyze the acetyl-CoA dependent formation of longer chain acyl-CoA products from octanoyl-CoA (Horie et al, Arch Biochem Biophys 274: 64-73 (1989); Hijikata et al, J Biol Chem 265, 4600-4606 (1990)).

Protein GenBank ID GI Number Organism AY308827.1:1 . . . AAQ77242.1 34597334 Helianthus 1350 annuus KAT2 Q56WD9.2 73919871 Arabidopsis thaliana KAT1 Q8LF48.2 73919870 Arabidopsis thaliana KAT5 Q570C8.2 73919872 Arabidopsis thaliana ACAA1 P09110.2 135751 Homo sapiens LCTHIO AAF04612.1 6165556 Sus scrofa Acaa1a NP_036621.1 6978429 Rattus norvegicus Acaa1b NP_001035108.1 90968642 Rattus norvegicus Acaa2 NP_569117.1 18426866 Rattus norvegicus

Acetoacetyl-CoA can also be synthesized from acetyl-CoA and malonyl-CoA by acetoacetyl-CoA synthase (EC 2.3.1.194). This enzyme (FhsA) has been characterized in the soil bacterium Streptomyces sp. CL190 where it participates in mevalonate biosynthesis (Okamura et al, PNAS USA 107:11265-70 (2010)). As this enzyme catalyzes an essentially irreversible reaction, it is particularly useful for metabolic engineering applications for overproducing metabolites, fuels or chemicals derived from acetoacetyl-CoA such as long chain alcohols. Other acetoacetyl-CoA synthase genes can be identified by sequence homology to fhsA. Acyl-CoA synthase enzymes such as fhsA and homologs can be engineered or evolved to accept longer acyl-CoA substrates by methods known in the art.

Protein GenBank ID GI Number Organism fhsA BAJ83474.1 325302227 Streptomyces sp CL190 AB183750.1:11991 . . . BAD86806.1 57753876 Streptomyces sp. KO-3988 12971 epzT ADQ43379.1 312190954 Streptomyces cinnamonensis ppzT CAX48662.1 238623523 Streptomyces anulatus O3I_22085 ZP_09840373.1 378817444 Nocardia brasiliensis

Chain length selectivity of selected thiolase enzymes described above is summarized in the table below.

Chain length Gene Organism C4 atoB Escherichia coli C6 phaD Pseudomonas putida C6-C8 bktB Ralstonia eutropha C10-C16 Acaa1a Rattus norvegicus

Step B. 3-Oxoacyl-CoA Reductase

3-Oxoacyl-CoA reductases (also known as 3-hydroxyacyl-CoA dehydrogenases, 3-ketoacyl-CoA reductases, beta-ketoacyl-CoA reductases, beta-hydroxyacyl-CoA dehydrogenases, hydroxyacyl-CoA dehydrogenases, and ketoacyl-CoA reductases) catalyze the reduction of 3-oxoacyl-CoA substrates to 3-hydroxyacyl-CoA products (FIG. 2B and FIG. 7B). These enzymes are often involved in fatty acid beta-oxidation and aromatic degradation pathways. For example, subunits of two fatty acid oxidation complexes in E. coli, encoded by fadB and fadJ, function as 3-hydroxyacyl-CoA dehydrogenases (Binstock et al., Methods Enzymol. 71 Pt C:403-411 (1981)). Knocking out a negative regulator encoded by fadR can be utilized to activate the fadB gene product (Sato et al., J Biosci. Bioeng 103:38-44 (2007)). Another 3-hydroxyacyl-CoA dehydrogenase from E. coli is paaH (Ismail et al., European Journal of Biochemistry 270:3047-3054 (2003)). Additional 3-oxoacyl-CoA enzymes include the gene products of phaC in Pseudomonas putida (Olivera et al., Proc. Natl. Acad. Sci U.S.A 95:6419-6424 (1998)) and paaC in Pseudomonas fluorescens (Di et al., 188:117-125 (2007)). These enzymes catalyze the reversible oxidation of 3-hydroxyadipyl-CoA to 3-oxoadipyl-CoA during the catabolism of phenylacetate or styrene. Other suitable enzyme candidates include AAO72312.1 from E. gracilis (Winkler et al., Plant Physiology 131:753-762 (2003)) and paaC from Pseudomonas putida (Olivera et al., PNAS USA 95:6419-6424 (1998)) Enzymes catalyzing the reduction of acetoacetyl-CoA to 3-hydroxybutyryl-CoA include hbd of Clostridium acetobutylicum (Youngleson et al., J Bacteriol. 171:6800-6807 (1989)), phbB from Zoogloea ramigera (Ploux et al., Eur. J Biochem. 174:177-182 (1988)), phaB from Rhodobacter sphaeroides (Alber et al., Mol. Microbiol 61:297-309 (2006)) and paaH1 of Ralstonia eutropha (Machado et al, Met Eng, In Press (2012)). The Z. ramigera enzyme is NADPH-dependent and also accepts 3-oxopropionyl-CoA as a substrate (Ploux et al., Eur. J Biochem. 174:177-182 (1988)). Additional genes include phaB in Paracoccus denitnficans, Hbd1 (C-terminal domain) and Hbd2 (N-terminal domain) in Clostridium kluyveri (Hillmer and Gottschalk, Biochim. Biophys. Acta 3334:12-23 (1974)) and HSD17B10 in Bos taurus (Wakil et al., J Biol. Chem. 207:631-638 (1954)). The enzyme from Paracoccus denitnficans has been functionally expressed and characterized in E. coli (Yabutani et al., FEMS Microbiol Lett. 133:85-90 (1995)). A number of similar enzymes have been found in other species of Clostridia and in Metallosphaera sedula (Berg et al., Science. 318:1782-1786 (2007)). The enzyme from Candida tropicalis is a component of the peroxisomal fatty acid beta-oxidation multifunctional enzyme type 2 (MFE-2). The dehydrogenase B domain of this protein is catalytically active on acetoacetyl-CoA. The domain has been functionally expressed in E. coli, a crystal structure is available, and the catalytic mechanism is well-understood (Ylianttila et al., Biochem Biophys Res Commun 324:25-30 (2004); Ylianttila et al., J Mol Biol 358:1286-1295 (2006)). 3-Hydroxyacyl-CoA dehydrogenases that accept longer acyl-CoA substrates (eg. EC 1.1.1.35) are typically involved in beta-oxidation. An example is HSD17B10 in Bos taurus (Wakil et al., J Biol. Chem. 207:631-638 (1954)). The pig liver enzyme is preferentially active on short and medium chain acyl-CoA substrates whereas the heart enzyme is less selective (He et al, Biochim Biophys Acta 1392:119-26 (1998)). The S. cerevisiae enzyme FOX2 is active in beta-degradation pathways and also has enoyl-CoA hydratase activity (Hiltunen et al, J Biol Chem 267: 6646-6653 (1992)).

Protein Genbank ID GI number Organism fadB P21177.2 119811 Escherichia coli fadJ P77399.1 3334437 Escherichia coli paaH NP_415913.1 16129356 Escherichia coli Hbd2 EDK34807.1 146348271 Clostridium kluyveri Hbd1 EDK32512.1 146345976 Clostridium kluyveri phaC NP_745425.1 26990000 Pseudomonas putida paaC ABF82235.1 106636095 Pseudomonas fluorescens HSD17B10 O02691.3 3183024 Bos taurus phbB P23238.1 130017 Zoogloea ramigera phaB YP_353825.1 77464321 Rhodobacter sphaeroides paaH1 CAJ91433.1 113525088 Ralstonia eutropha phaB BAA08358 675524 Paracoccus denitrificans Hbd NP_349314.1 15895965 Clostridium acetobutylicum Hbd AAM14586.1 20162442 Clostridium beijerinckii Msed_1423 YP_001191505 146304189 Metallosphaera sedula Msed_0399 YP_001190500 146303184 Metallosphaera sedula Msed_0389 YP_001190490 146303174 Metallosphaera sedula Msed_1993 YP_001192057 146304741 Metallosphaera sedula Fox2 Q02207 399508 Candida tropicalis HSD17B10 O02691.3 3183024 Bos taurus HADH NP_999496.1 47523722 Bos taurus 3HCDH AAO72312.1 29293591 Euglena gracilis FOX2 NP_012934.1 6322861 Saccharomyces cerevisiae

Chain length specificity of selected hydroxyacyl-CoA dehydrogenase enzymes is shown below. Directed evolution can enhance selectivity of enzymes for longer-chain substrates. For example, Machado and coworkers developed a selection platform for directed evolution of chain elongation enzymes that favor longer acyl-CoA substrates. This group evolved paaH1 of Ralstonia eutropha for improved activity on 3-oxo-hexanoyl-CoA (Machado et al, Met Eng, In Press (2012)).

Chain length Gene Organism C4 hbd Clostridium acetobutylicum C5 phbB Zoogloea ramigera C4-C6  paaH1 Ralstonia eutropha C4-C10 HADH Sus scrofa C4-C18 fadB Escherichia coli

Step C. 3-Hydroxyacyl-CoA Dehydratase

3-Hydroxyacyl-CoA dehydratases (eg. EC 4.2.1.17, also known as enoyl-CoA hydratases) catalyze the dehydration of a range of 3-hydroxyacyl-CoA substrates (Roberts et al., Arch. Microbiol 117:99-108 (1978); Agnihotri et al., Bioorg. Med. Chem. 11:9-20 (2003); Conrad et al., J Bacteriol. 118:103-111(1974)) and can be used in the conversion of 3-hydroxyacyl-CoA to enoyl-CoA (FIGS. 2C and 7C). The ech gene product of Pseudomonas putida catalyzes the conversion of 3-hydroxybutyryl-CoA to crotonyl-CoA (Roberts et al., Arch. Microbiol 117:99-108 (1978)). This transformation is also catalyzed by the crt gene product of Clostridium acetobutylicum, the crt1 gene product of C. kluyveri, and other clostridial organisms Atsumi et al., Metab Eng 10:305-311(2008); Boynton et al., J Bacteriol. 178:3015-3024 (1996); Hillmer et al., FEBS Lett. 21:351-354 (1972)). Additional enoyl-CoA hydratase candidates are phaA and phaB, of P. putida, and paaA and paaB from P. fluorescens (Olivera et al., Proc. Natl. Acad. Sci U.S.A 95:6419-6424 (1998)). The gene product of pimF in Rhodopseudomonas palustris is predicted to encode an enoyl-CoA hydratase that participates in pimeloyl-CoA degradation (Harrison et al., Microbiology 151:727-736 (2005)). Lastly, a number of Escherichia coli genes have been shown to demonstrate enoyl-CoA hydratase functionality including maoC (Park et al., J Bacteriol. 185:5391-5397 (2003)), paaF (Ismail et al., Eur. J Biochem. 270:3047-3054 (2003); Park et al., Appl. Biochem. Biotechnol 113-116:335-346 (2004); Park et al., Biotechnol Bioeng 86:681-686 (2004)) and paaG (Ismail et al., Eur. J Biochem. 270:3047-3054 (2003); Park and Lee, Appl. Biochem. Biotechnol 113-116:335-346 (2004); Park and Yup, Biotechnol Bioeng 86:681-686 (2004)) Enzymes with 3-hydroxyacyl-CoA dehydratase activity in S. cerevisiae include PHS 1 and FOX2.

GenBank Gene Accession No. GI No. Organism ech NP_745498.1 26990073 Pseudomonas putida crt NP_349318.1 15895969 Clostridium acetobutylicum crt1 YP_001393856 153953091 Clostridium kluyveri phaA ABF82233.1 26990002 Pseudomonas putida phaB ABF82234.1 26990001 Pseudomonas putida paaA NP_745427.1 106636093 Pseudomonas fluorescens paaB NP_745426.1 106636094 Pseudomonas fluorescens pimF CAE29158.1 39650635 Rhodopseudomonas palustris maoC NP_415905.1 16129348 Escherichia coli paaF NP_415911.1 16129354 Escherichia coli paaG NP_415912.1 16129355 Escherichia coli FOX2 NP_012934.1 6322861 Saccharomyces cerevisiae PHS1 NP_012438.1 6322364 Saccharomyces cerevisiae

Enoyl-CoA hydratases involved in beta-oxidation can also be used in an fatty alcohol, fatty aldehyde and fatty acid biosynthetic pathway. For example, the multifunctional MFP2 gene product of Arabidopsis thaliana exhibits an enoyl-CoA reductase activity selective for chain lengths less than or equal to C14 (Arent et al, J Biol Chem 285:24066-77 (2010)). Alternatively, the E. coli gene products of fadA and fadB encode a multienzyme complex involved in fatty acid oxidation that exhibits enoyl-CoA hydratase activity (Yang et al., Biochemistry 30:6788-6795 (1991); Yang, J Bacteriol. 173:7405-7406 (1991); Nakahigashi et al., Nucleic Acids Res. 18:4937 (1990)). The fadI and fadJ genes encode similar functions and are naturally expressed under anaerobic conditions (Campbell et al., Mol. Microbiol 47:793-805 (2003)).

Protein GenBank ID GI Number Organism MFP2 AAD18042.1 4337027 Arabidopsis thaliana fadA YP_026272.1 49176430 Escherichia coli fadB NP_418288.1 16131692 Escherichia coli fadI NP_416844.1 16130275 Escherichia coli fadJ NP_416843.1 16130274 Escherichia coli fadR NP_415705.1 16129150 Escherichia coli

Chain length specificity of selected 3-hydroxyacyl-CoA dehydratase enzymes is shown below.

Chain length Gene Organism C4-C6 crt Clostridium acetobutylicum C4-C7 pimF Rhodopseudomonas palustris  C4-C14 MFP2 Arabidopsis thaliana

Step D. Enoyl-CoA Reductase

Enoyl-CoA reductases (also known as acyl-CoA dehydrogenases, trans-2-enoyl-CoA reductases, or acyl-CoA oxidoreductases) catalyze the conversion of an enoyl-CoA to an acyl-CoA (step D of FIGS. 2 and 7). Exemplary acyl-CoA dehydrogenase or enoyl-CoA reductase (ECR) enzymes are the gene products of fadE of E. coli and Salmonella enterica (Iram et al, J Bacteriol 188:599-608 (2006)). YdiO of E. coli encodes a ferridoxin-dependent enoyl-CoA reductase (Dellomonaco et al Nature 476:355 (2011)). The bcd gene product from Clostridium acetobutylicum (Atsumi et al., 10:305-311(2008); Boynton et al., J Bacteriol 178:3015-3024 (1996)) catalyzes the reduction of crotonyl-CoA to butyryl-CoA (EC 1.3.99.2). This enzyme participates in the acetyl-CoA fermentation pathway to butyrate in Clostridial species (Jones et al., Microbiol Rev. 50:484-524 (1986)). Activity of butyryl-CoA reductase can be enhanced by expressing bcd in conjunction with expression of the C. acetobutylicum etfAB genes, which encode an electron transfer flavoprotein. An additional candidate for the enoyl-CoA reductase step is the enoyl-CoA reductase (EC 1.3.1.44) 1 ER from E. gracilis (Hoffmeister et al., J Biol. Chem 280:4329-4338 (2005)). A construct derived from this sequence following the removal of its mitochondrial targeting leader sequence was cloned in E. coli resulting in an active enzyme. A close homolog of the ECR protein from the prokaryote Treponema denticola, encoded by TDE0597, has also been cloned and expressed in E. coli (Tucci et al., FEBS Lett, 581:1561-1566 (2007)). Six genes in Syntrophus aciditrophicus were identified by sequence homology to the C. acetobutylicum bcd gene product. The S. aciditrophicus genes syn_02637 and syn_02636 bear high sequence homology to the etfAB genes of C. acetobutylicum, and are predicted to encode the alpha and beta subunits of an electron transfer flavoprotein.

Protein GenBank ID GI Number Organism fadE AAC73325.2 87081702 Escherichia coli ydiO YP_489957.1 4E+08 Escherichia coli fadE YP_005241256.1 379699528 Salmonella enterica bcd NP_349317.1 15895968 Clostridium acetobutylicum etfA NP_349315.1 15895966 Clostridium acetobutylicum etfB NP_349316.1 15895967 Clostridium acetobutylicum TER Q5EU90.1 62287512 Euglena gracilis TER NP_612558.1 19924091 Rattus norvegicus TDE0597 NP_971211.1 42526113 Treponema denticola syn_02587 ABC76101 85721158 Syntrophus aciditrophicus syn_02586 ABC76100 85721157 Syntrophus aciditrophicus syn_01146 ABC76260 85721317 Syntrophus aciditrophicus syn_00480 ABC77899 85722956 Syntrophus aciditrophicus syn_02128 ABC76949 85722006 Syntrophus aciditrophicus syn_01699 ABC78863 85723920 Syntrophus aciditrophicus syn_02637 ABC78522.1 85723579 Syntrophus aciditrophicus syn_02636 ABC78523.1 85723580 Syntrophus aciditrophicus

Additional enoyl-CoA reductase enzyme candidates are found in organisms that degrade aromatic compounds. Rhodopseudomonas palustris, a model organism for benzoate degradation, has the enzymatic capability to degrade pimelate via beta-oxidation of pimeloyl-CoA. Adjacent genes in the pim operon, pimC and pimD, bear sequence homology to C. acetobutylicum bcd and are predicted to encode a flavin-containing pimeloyl-CoA dehydrogenase (Harrison et al., 151:727-736 (2005)). The genome of nitrogen-fixing soybean symbiont Bradyrhizobium japonicum also contains a pim operon composed of genes with high sequence similarity to pimC and pimD of R. palustris (Harrison and Harwood, Microbiology 151:727-736 (2005)).

Protein GenBank ID GI Number Organism pimC CAE29155 39650632 Rhodopseudomonas palustris pimD CAE29154 39650631 Rhodopseudomonas palustris pimC BAC53083 27356102 Bradyrhizobium japonicum pimD BAC53082 27356101 Bradyrhizobium japonicum

An additional candidate is 2-methyl-branched chain enoyl-CoA reductase (EC 1.3.1.52 and EC 1.3.99.12), an enzyme catalyzing the reduction of sterically hindered trans-enoyl-CoA substrates. This enzyme participates in branched-chain fatty acid synthesis in the nematode Ascaris suum and is capable of reducing a variety of linear and branched chain substrates including 2-methylvaleryl-CoA, 2-methylbutanoyl-CoA, 2-methylpentanoyl-CoA, octanoyl-CoA and pentanoyl-CoA (Duran et al., 268:22391-22396 (1993)). Two isoforms of the enzyme, encoded by genes acad1 and acad, have been characterized.

Protein GenBank ID GI Number Organism acad1 AAC48316.1 2407655 Ascaris suum acad AAA16096.1 347404 Ascaris suum

At least three mitochondrial enoyl-CoA reductase enzymes exist in E. gracilis and are applicable for use in the invention. Three mitochondrial enoyl-CoA reductase enzymes of E. gracilis (ECR1-3) exhibit different chain length preferences (Inui et al., European Journal of Biochemistry 142:121-126 (1984)), which is particularly useful for dictating the chain length of the desired fatty alcohol, fatty aldehyde or fatty acid products. EST's ELL00002199, ELL00002335, and ELL00002648, which are all annotated as mitochondrial trans-2-enoyl-CoA reductases, can be used to isolate these additional enoyl-CoA reductase genes by methods known in the art. Two ECR enzymes from rat liver microsomes also exhibit different substrate specificities (Nagi et al, Arch Biochem Biophys 226:50-64 (1983)). The sequences of these enzymes have not been identified to date. The Mycobacterium smegmatis enoyl-CoA reductase accepts acyl-CoA substrates of chain lengths between C10-C16 (Shimakata et al, J Biochem 89:1075-80 (1981)).

Protein GenBank ID GI Number Organism acad WP_015308343.1 5E+08 Mycobacterium smegmatis caiA WP_015308454.1 5E+08 Mycobacterium smegmatis

Enoyl-CoA reductases and their chain length specificities are shown in the table below.

Chain length Gene Organism C4-C6 ECR1 Euglena gracilis C6-C8 ECR3 Euglena gracilis C8-10  ECR2 Euglena gracilis  C8-C16 Long chain ECR Rattus norvegicus C10-C16 ECR (acad; caiA) Mycobacterium smegmatis  C2-C18 fadE Salmonella enterica

Step E. Acyl-CoA Reductase (Aldehyde Forming)

Reduction of an acyl-CoA to a fatty alcohol is catalyzed by either a single enzyme or pair of enzymes that exhibit acyl-CoA reductase and alcohol dehydrogenase activities. Acyl-CoA dehydrogenases that reduce an acyl-CoA to its corresponding aldehyde include fatty acyl-CoA reductase (EC 1.2.1.42, 1.2.1.50), succinyl-CoA reductase (EC 1.2.1.76), acetyl-CoA reductase, butyryl-CoA reductase and propionyl-CoA reductase (EC 1.2.1.3). Aldehyde forming acyl-CoA reductase enzymes with demonstrated activity on acyl-CoA, 3-hydroxyacyl-CoA and 3-oxoacyl-CoA substrates are known in the literature. Several acyl-CoA reductase enzymes are active on 3-hydroxyacyl-CoA substrates. For example, some butyryl-CoA reductases from Clostridial organisms, are active on 3-hydroxybutyryl-CoA and propionyl-CoA reductase of L. reuteri is active on 3-hydroxypropionyl-CoA. An enzyme for converting 3-oxoacyl-CoA substrates to their corresponding aldehydes is malonyl-CoA reductase Enzymes in this class that demonstrate activity on enoyl-CoA substrates have not been identified to date. Specificity for a particular substrate can be refined using evolution or enzyme engineering methods known in the art.

Exemplary fatty acyl-CoA reductases enzymes are encoded by acr1 of Acinetobacter calcoaceticus (Reiser, Journal of Bacteriology 179:2969-2975 (1997)) and Acinetobacter sp. M-1 (Ishige et al., Appl. Environ. Microbiol. 68:1192-1195 (2002)). Two gene products from Mycobacterium tuberculosis accept longer chain fatty acyl-CoA substrates of length C16-C18 (Harminder Singh, U. Central Florida (2007)). Yet another fatty acyl-CoA reductase is LuxC of Photobacterium phosphoreum (Lee et al, Biochim Biohys Acta 1388:215-22 (1997)) Enzymes with succinyl-CoA reductase activity are encoded by sucD of Clostridium kluyveri (Sohling, J. Bacteriol. 178:871-880 (1996)) and sucD of P. gingivalis (Takahashi, J. Bacteriol 182:4704-4710 (2000)). Additional succinyl-CoA reductase enzymes participate in the 3-hydroxypropionate/4-hydroxybutyrate cycle of thermophilic archaea including Metallosphaera sedula (Berg et al., Science 318:1782-1786 (2007)) and Thermoproteus neutrophilus (Ramos-Vera et al., J Bacteriol, 191:4286-4297 (2009)). The M. sedula enzyme, encoded by Msed_0709, is strictly NADPH-dependent and also has malonyl-CoA reductase activity. The T. neutrophilus enzyme is active with both NADPH and NADH. The enzyme acylating acetaldehyde dehydrogenase in Pseudomonas sp, encoded by bphG, is yet another as it has been demonstrated to oxidize and acylate acetaldehyde, propionaldehyde, butyraldehyde, isobutyraldehyde and formaldehyde (Powlowski, J. Bacteriol. 175:377-385 (1993)). In addition to reducing acetyl-CoA to ethanol, the enzyme encoded by adhE in Leuconostoc mesenteroides has been shown to oxidize the branched chain compound isobutyraldehyde to isobutyryl-CoA (Kazahaya, J. Gen. Appl. Microbiol. 18:43-55 (1972); and Koo et al., Biotechnol Lett. 27:505-510 (2005)). Butyraldehyde dehydrogenase catalyzes a similar reaction, conversion of butyryl-CoA to butyraldehyde, in solventogenic organisms such as Clostridium saccharoperbutylacetonicum (Kosaka et al., Biosci Biotechnol Biochem., 71:58-68 (2007)). Exemplary propionyl-CoA reductase enzymes include pduP of Salmonella typhimurium LT2 (Leal, Arch. Microbiol. 180:353-361 (2003)) and eutE from E. coli (Skraly, WO Patent No. 2004/024876). The propionyl-CoA reductase of Salmonella typhimurium LT2, which naturally converts propionyl-CoA to propionaldehyde, also catalyzes the reduction of 5-hydroxyvaleryl-CoA to 5-hydroxypentanal (WO 2010/068953A2). The propionaldehyde dehydrogenase of Lactobacillus reuteri, PduP, has a broad substrate range that includes butyraldehyde, valeraldehyde and 3-hydroxypropionaldehyde (Luo et al, Appl Microbiol Biotech, 89: 697-703 (2011). Additional FAR enzymes are encoded by wax2 of Arabidopsis thaliana and FAR1 and FAR2 of Mus musculus (Chen et al, Plant Cell 15:1170-85 (2003); Cheng and Russel, J Biol Chem 279:37789-97 (2004)). Both mouse FAR enzymes accept substrates with a chain length of C16-18. Additionally, some acyl-ACP reductase enzymes such as the orf1594 gene product of Synechococcus elongatus PCC7942 also exhibit aldehyde-forming acyl-CoA reductase activity (Schirmer et al, Science, 329: 559-62 (2010)). Acyl-ACP reductase enzymes and homologs are described in further detail in Example XII.

Protein GenBank ID GI Number Organism acr1 YP_047869.1 50086359 Acinetobacter calcoaceticus acr1 AAC45217 1684886 Acinetobacter baylyi acr1 BAB85476.1 18857901 Acinetobacter sp. Strain M-1 Rv1543 NP_216059.1 15608681 Mycobacterium tuberculosis Rv3391 NP_217908.1 15610527 Mycobacterium tuberculosis LuxC Q03324 547874 Photobacterium leiognathi PL741 LuxC AAT00788.1 46561111 Photobacterium phosphoreum Msed_0709 YP_001190808.1 146303492 Metallosphaera sedula Tneu_0421 ACB39369.1 170934108 Thermoproteus neutrophilus sucD P38947.1 172046062 Clostridium kluyveri sucD NP_904963.1 34540484 Porphyromonas gingivalis bphG BAA03892.1 425213 Pseudomonas sp adhE AAV66076.1 55818563 Leuconostoc mesenteroides bld AAP42563.1 31075383 Clostridium saccharoperbutyl- acetonicum pduP NP_460996 16765381 Salmonella typhimurium LT2 eutE NP_416950 16130380 Escherichia coli pduP CCC03595.1 337728491 Lactobacillus reuteri wax2 AAN06975.1 22900949 Arabidopsis thaliana FAR1 AAH07178.1 13938126 Mus musculus FAR2 AAH55759 33416982 Mus musculus

An additional enzyme type that converts an acyl-CoA to its corresponding aldehyde is malonyl-CoA reductase which transforms malonyl-CoA to malonic semialdehyde. Malonyl-CoA reductase is a key enzyme in autotrophic carbon fixation via the 3-hydroxypropionate cycle in thermoacidophilic archaeal bacteria (Berg, Science 318:1782-1786 (2007); and Thauer, Science 318:1732-1733 (2007)). The enzyme utilizes NADPH as a cofactor and has been characterized in Metallosphaera and Sulfolobus sp. (Alber et al., J. Bacteriol. 188:8551-8559 (2006); and Hugler, J. Bacteriol. 184:2404-2410 (2002)). The enzyme is encoded by Msed_0709 in Metallosphaera sedula (Alber et al., J. Bacteriol. 188:8551-8559 (2006); and Berg, Science 318:1782-1786 (2007)). A gene encoding a malonyl-CoA reductase from Sulfolobus tokodaii was cloned and heterologously expressed in E. coli (Alber et al., J. Bacteriol 188:8551-8559 (2006). This enzyme has also been shown to catalyze the conversion of methylmalonyl-CoA to its corresponding aldehyde (WO2007141208 (2007)). Although the aldehyde dehydrogenase functionality of these enzymes is similar to the bifunctional dehydrogenase from Chloroflexus aurantiacus, there is little sequence similarity. Both malonyl-CoA reductase enzyme candidates have high sequence similarity to aspartate-semialdehyde dehydrogenase, an enzyme catalyzing the reduction and concurrent dephosphorylation of aspartyl-4-phosphate to aspartate semialdehyde. Additional gene candidates can be found by sequence homology to proteins in other organisms including Sulfolobus solfataricus and Sulfolobus acidocaldarius and have been listed below. Yet another candidate for CoA-acylating aldehyde dehydrogenase is the ald gene from Clostridium beijerinckii (Toth, Appl. Environ. Microbiol. 65:4973-4980 (1999). This enzyme has been reported to reduce acetyl-CoA and butyryl-CoA to their corresponding aldehydes. This gene is very similar to eutE that encodes acetaldehyde dehydrogenase of Salmonella typhimurium and E. coli (Toth, Appl. Environ. Microbiol. 65:4973-4980 (1999).

Gene GenBank ID GI Number Organism Msed_0709 YP_001190808.1 146303492 Metallosphaera sedula mcr NP_378167.1 15922498 Sulfolobus tokodaii asd-2 NP_343563.1 15898958 Sulfolobus solfataricus Saci_2370 YP_256941.1 70608071 Sulfolobus acidocaldarius Ald AAT66436 49473535 Clostridium beijerinckii eutE AAA80209 687645 Salmonella typhimurium eutE NP_416950 16130380 Escherichia coli

Chain length specificity ranges of selected aldehyde-forming acyl-CoA reductase enzymes are show in the table below.

Chain length Gene Organism C2-C4 bphG Pseudomonas sp C4  Bld Clostridium saccharoperbutylacetonicum C12-C20 ACR Acinetobacter calcoaceticus C14-C18 Acr1 Acinetobacter sp. Strain M-1 C16-C18 Rv1543, Mycobacterium tuberculosis Rv3391 C16-C18 FAR1, FAR2 Mus musculus C18 Wax2 Arabidopsis thaliana

Step G. Acyl-CoA Reductase (Alcohol Forming)

Bifunctional alcohol-forming acyl-CoA reductase enzymes catalyze step G (i.e. step E and F) of FIGS. 2 and 7. Enzymes with this activity include adhE of E. coli (Kessler et al., FEBS. Lett. 281:59-63 (1991))) and adhE2 of Clostridium acetobutylicum (Fontaine et al., J. Bacteriol. 184:821-830 (2002))). The E. coli enzyme is active on C2 substrates, whereas the C. acetobutylicum enzyme has a broad substrate range that spans C2-C8 (Dekishima et al, J Am Chem Soc 133:11399-11401(2011)). The C. acetobutylicum enzymes encoded by bdh I and bdh II (Walter, et al., J. Bacteriol. 174:7149-7158 (1992)), reduce acetyl-CoA and butyryl-CoA to ethanol and butanol, respectively. The adhE gene produce from Leuconostoc mesenteroides is active on acetyl-CoA and isobutyryl-CoA (Kazahaya et al., J. Gen. Appl. Microbiol. 18:43-55 (1972); Koo et al., Biotechnol Lett, 27:505-510 (2005)) Enzyme candidates in other organisms including Roseiflexus castenholzii, Erythrobacter sp. NAP1 and marine gamma proteobacterium HTCC2080 can be inferred by sequence similarity. Longer chain acyl-CoA molecules can be reduced to their corresponding alcohols by enzymes such as the jojoba (Simmondsia chinensis) FAR which encodes an alcohol-forming fatty acyl-CoA reductase. Its overexpression in E. coli resulted in FAR activity and the accumulation of C16-C18 fatty alcohols (Metz et al., Plant Physiol, 122:635-644 (2000)). FAR enzymes in Arabidopsis thaliana include the gene products of At3g11980, At3g44560 and CER4 (Doan et al, J Plant Physiol 166 (2006); Rowland et al, Plant Physiol 142:866-77 (2006)). Bifunctional prokaryotic FAR enzymes are found in Marinobacter aquaeolei VT8 (Hofvander et al, FEBS Lett 3538-43 (2011)), Marinobacter algicola and Oceanobacter strain RED65 (US Pat Appl 20110000125). Other suitable enzymes include bfar from Bombyx mori, mfar1 and mfar2 from Mus musculus; mfar2 from Mus musculus; acrM1 from Acinetobacter sp. M1; and hfar from H. sapiens.

Protein GenBank ID GI Number Organism adhE NP_415757.1 16129202 Escherichia coli adhE2 AAK09379.1 12958626 Clostridium acetobutylicum bdh I NP_349892.1 15896543 Clostridium acetobutylicum bdh II NP_349891.1 15896542 Clostridium acetobutylicum adhE AAV66076.1 55818563 Leuconostoc mesenteroides mcr AAS20429.1 42561982 Chloroflexus aurantiacus Rcas_2929 YP_001433009.1 156742880 Roseiflexus castenholzii NAP1_02720 ZP_01039179.1 85708113 Erythrobacter sp. NAP1 MGP2080_00535 ZP_01626393.1 119504313 marine gamma proteobacterium HTCC2080 FAR AAD38039.1 5020215 Simmondsia chinensis At3g11980 NP_191229.1 15228993 Arabidopsis thaliana At3g44560 NP_190042.2 145339120 Arabidopsis thaliana CER4 AEE86278.1 332660878 Arabidopsis thaliana FAR YP_959486.1 120555135 Marinobacter aquaeolei bfar Q8R079 81901336 Bombyx mori

Chain length specificity ranges of selected alcohol-forming acyl-CoA reductase enzymes are show in the table below.

Chain length Gene Organism C2  adhE Escherichia coli C2-C8 adhe2 Clostridium acetobutylicum C14-C16 At3g11980 Arabidopsis thaliana C16 At3g44560 Arabidopsis thaliana C16-C18 FAR Simmondsia chinensis C14-C18 FAR Marinobacter aquaeolei C24-C26 CER4 Arabidopsis thaliana

Step F. Fatty Aldehyde Reductase

Exemplary genes encoding enzymes that catalyze the conversion of an aldehyde to alcohol (i.e., alcohol dehydrogenase or equivalently aldehyde reductase) include alrA encoding a medium-chain alcohol dehydrogenase for C2-C14 (Tani et al., Appl. Environ. Microbiol. 66:5231-5235 (2000)), yqhD and fucO from E. coli (Sulzenbacher et al., 342:489-502 (2004)), and bdh I and bdh II from C. acetobutylicum which converts butyryaldehyde into butanol (Walter et al., J Bacteriol 174:7149-7158 (1992)). The afrA gene product showed no activity on aldehydes longer than C14, and favored the reductive direction (Tani et al, supra). YqhD catalyzes the reduction of a wide range of aldehydes using NADPH as the cofactor, with a preference for chain lengths longer than C(3) (Sulzenbacher et al, J Mol Biol 342:489-502 (2004); Perez et al., J Biol. Chem. 283:7346-7353 (2008)). The adhA gene product from Zymomonas mobilis has been demonstrated to have activity on a number of aldehydes including formaldehyde, acetaldehyde, propionaldehyde, butyraldehyde, and acrolein (Kinoshita et al., Appl Microbiol Biotechnol 22:249-254 (1985)). Additional aldehyde reductase candidates are encoded by bdh in C. saccharoperbutylacetonicum and Cbei_1722, Cbei_2181 and Cbei_2421 in C. beijerinckii. The alcohol dehydrogenase from Leifsonia sp. S749 shows maximal activity on medium chain-length substrates of length C6-C7 (Inoue et al, AEM71: 3633-3641 (2005). The adh gene product of Pseudomonas putida is active on substrates of length C3-C10 (Nagashima et al, J Ferment Bioeng 82:328-33(1996)). The alcohol dehydrogenase enzymes ADH1 and ADH2 of Geobacillus thermodenitrificans oxidize alcohols up to a chain length of C30 (Liu et al, Physiol Biochem 155:2078-85 (2009)). Three additional alcohol dehydrogenase enzymes from Geobacillus thermodenitrificans are active on C2-C14 substrates (Liu et al, supra).

Protein GenBank ID GI Number Organism alrA BAB12273.1 9967138 Acinetobacter sp. strain M-1 ADH2 NP_014032.1 6323961 Saccharomyces cerevisiae yqhD NP_417484.1 16130909 Escherichia coli fucO NP_417279.1 16130706 Escherichia coli bdh I NP_349892.1 15896543 Clostridium acetobutylicum bdh II NP_349891.1 15896542 Clostridium acetobutylicum adhA YP_162971.1 56552132 Zymomonas mobilis bdh BAF45463.1 124221917 Clostridium saccharoperbutyl- acetonicum Cbei_1722 YP_001308850 150016596 Clostridium beijerinckii Cbei_2181 YP_001309304 150017050 Clostridium beijerinckii Cbei_2421 YP_001309535 150017281 Clostridium beijerinckii lsadh BAD99642.1 67625613 Leifsonia sp. S749 adh Pseudomonas putida ADH1 YP_001126968.1 138896515 Geobacillus thermodenitrificans ADH2 YP_001125863.1 138895410 Geobacillus thermodenitrificans GTNG_0872 YP_001124995.1 138894542 Geobacillus thermodenitrificans GTNG_1287 YP_001125402.1 138894949 Geobacillus thermodenitrificans GTNG_1851 YP_001125956.1 138895503 Geobacillus thermodenitrificans

Native alcohol dehydrogenases also convert aldehyde substrates to alcohol products. To date, seven alcohol dehydrogenases, ADHI-ADHVII, have been reported in S. cerevisiae (de Smidt et al, FEMS Yeast Res 8:967-78 (2008)). ADH1 (GI:1419926) is the key enzyme responsible for reducing acetaldehyde to ethanol in the cytosol under anaerobic conditions. In K. lactis, two NAD-dependent cytosolic alcohol dehydrogenases have been identified and characterized. These genes also show activity for other aliphatic alcohols. The genes ADH1 (GI:113358) and ADHII (GI:51704293) are preferentially expressed in glucose-grown cells (Bozzi et al, Biochim Biophys Acta 1339:133-142 (1997)). Cytosolic alcohol dehydrogenases are encoded by ADH1 (GI:608690) in C. albicans, ADH1 (GI:3810864) in S. pombe, ADH1 (GI:5802617) in Y. lipolytica, ADH1 (GI:2114038) and ADH11 (GI:2143328) in Pichia stipitis or Scheffersomyces stipitis (Passoth et al, Yeast 14:1311-23 (1998)). Candidate alcohol dehydrogenases are shown the table below.

Protein GenBank ID GI number Organism SADH BAA24528.1 2815409 Candida parapsilosis ADH1 NP_014555.1 6324486 Saccharomyces cerevisiae s288c ADH2 NP_014032.1 6323961 Saccharomyces cerevisiae s288c ADH3 NP_013800.1 6323729 Saccharomyces cerevisiae s288c ADH4 NP_011258.2 269970305 Saccharomyces cerevisiae s288c ADH5 NP_010113.1 6320033 Saccharomyces cerevisiae s288c (SFA1) ADH6 NP_014051.1 6323980 Saccharomyces cerevisiae s288c ADH7 NP_010030.1 6319949 Saccharomyces cerevisiae s288c adhP CAA44614.1 2810 Kluyveromyces lactis ADH1 P20369.1 113358 Kluyveromyces lactis ADH2 CAA45739.1 2833 Kluyveromyces lactis ADH3 P49384.2 51704294 Kluyveromyces lactis

Substrate specificity ranges of selected alcohol dehydrogenase enzymes are show in the table below.

Chain length Gene Organism C6-C7  lsadh Leifsonia sp. S749 C2-C8  yqhD Escherichia coli C3-C10 Adh Pseudomonas putida C2-C14 alrA Acinetobacter sp. strain M-1 C2-C14 ADH Geobacillus thermodenitrificans C2-C30 ADH1 Geobacillus thermodenitrificans

Step O. Elongase

Elongase (ELO) enzymes utilize malonyl-CoA to add a C2 unit to a growing acyl-CoA chain. This process also involves decarboxylation and is thus largely irreversible. Trypanosoma brucei, a eukaryotic human parasite, is known to produce long chain fatty acids using an elongase system. The process is initiated by butyryl-CoA. In particular, the ELO system esterifies the growing fatty acid chain to CoA intermediates rather than ACP intermediates like the bacterial and other microbial counterparts (Lee et al, Cell 126, 691-699, 2006; Cronan, Cell, 126, 2006). This is in contrast to typical bacterial fatty acid elongation which is initiated following the formation of acetoacetyl acyl-ACP from malonyl-ACP. So far, four ELOs (encoded by ELO1-4) that are homologous to their animal counterparts have been found in T. brucei (Lee et al, Nature Reviews Microbiology, Vol 5, 287-297, 2007). ELO1-3 together account for synthesis of saturated fatty acids up to a chain length of C18. ELO1 converts C4 to C10, ELO2 extends the chain length from C10 to myristate (C14), and ELO3 extends myristate to C18. There is some overlap in ELO specificity; for example, ELO1 can extend a C10 primer to C12, albeit with low activity. ELO4 is an example of an ELO that is specific for poly unsaturated fatty acids (PUFAs). It extends arachidonate (C20:4) by two carbon atoms. Several additional ELO enzymes can be found by sequence homology (see Lee et al, Nature Reviews Microbiology, Vol 5, 287-297, 2007).

Elongase enzymes are found in several compartments including the mitochondria, endoplasmic reticulum, proteoliposomes and peroxisomes. For example, some yeast such as S. cerevisiae are able to synthesize long-chain fatty acids of chain length C16 and higher via a mitochondrial elongase which accepts exogenous or endogenous acyl-CoA substrates (Bessoule et al, FEBS Lett 214: 158-162 (1987)). This system requires ATP for activity. The endoplasmic reticulum also has an elongase system for synthesizing very long chain fatty acids (C18+) from acyl-CoA substrates of varying lengths (Kohlwein et al, Mol Cell Biol 21:109-25 (2001)). Genes involved in this system include TSC13, ELO2 and ELO3. ELO1 catalyzes the elongation of C12 acyl-CoAs to C16-C18 fatty acids.

Protein Accession # GI number Organism ELO2 NP_009963.1 6319882 Saccharomyces cerevisiae ELO3 NP_013476.3 398366027 Saccharomyces cerevisiae TSC13 NP_010269.1 6320189 Saccharomyces cerevisiae ELO1 NP_012339.1 6322265 Saccharomyces cerevisiae ELO1 AAX70671.1 62176566 Trypanosoma brucei ELO2 AAX70672.1 62176567 Trypanosoma brucei ELO3 AAX70673.1 62176568 Trypanosoma brucei ELO4 AAX70768.1 62176665 Trypanosoma brucei ELO4 AAX69821.1 62175690 Trypanosoma brucei

Those skilled in the art also can obtain nucleic acids encoding any or all of the malonyl-CoA independent FAS pathway or acyl-reduction pathway enzymes by cloning using known sequences from available sources. For example, any or all of the encoding nucleic acids for the malonyl-CoA independent FAS pathway can be readily obtained using methods well known in the art from E. gracilis as this pathway has been well characterized in this organism. E. gracilis encoding nucleic acids can be isolated from, for example, an E. gracilis cDNA library using probes of known sequence. The probes can be designed with whole or partial DNA sequences from the following EST sequences from the publically available sequence database TBestDB (http://tbestdb.bcm.umontreal.ca). The nucleic acids generated from this process can be inserted into an appropriate expression vector and transformed into E. coli or other microorganisms to generate fatty alcohols, fatty aldehydes or fatty acids production organisms of the invention.

Thiolase (FIG. 2A): ELL00002550, ELL00002493, ELL00000789

3-Hydroxyacyl-CoA dehydrogenase (FIG. 2B): ELL00000206, ELL00002419, ELL00006286, ELL00006656

Enoyl-CoA hydratase (FIG. 2C): ELL00005926, ELL00001952, ELL00002235, ELL00006206

Enoyl-CoA reductase (FIG. 2D): ELL00002199, ELL00002335, ELL00002648

Acyl-CoA reductase (FIG. 2E; 2E/F): ELL00002572, ELL00002581, ELL00000108

Alternatively, the above EST sequences can be used to identify homologue polypeptides in GenBank through BLAST search. The resulting homologue polypeptides and their corresponding gene sequences provide additional encoding nucleic acids for transformation into E. coli or other microorganisms to generate the fatty alcohols, fatty aldehydes or fatty acids producing organisms of the invention. Listed below are exemplary homologue polypeptide and their gene accession numbers in GenBank which are applicable for use in the non-naturally occurring organisms of the invention.

Ketoacyl-CoA acyltransferase (or ketoacyl-CoA thiolase)

Protein GenBank ID GI number Organism Dole_2160 YP_001530041 158522171 Desulfococcus oleovorans Hxd3 DalkDRAFT_1939 ZP_02133627 163726110 Desulfatibacillum alkenivorans AK-01 BSG1_09488 ZP_01860900 149182424 Bacillus sp. SG-1

3-Hydroxyacyl-CoA dehydrogenase

Protein GenBank ID GI number Organism AaeL_AAEL002841 XP_001655993 157132312 Aedes aegypti hadh NP_001011073 58331907 Xenopus tropicalis hadh NP_001003515 51011113 Danio rerio

Enoyl-CoA hydratase

Protein GenBank ID GI number Organism Tb927.3.4850 XP_844077 72387305 Trypanosoma brucei Tc00.1047053509701.10 XP_802711 71399112 Trypanosoma cruzi strain CL Brener PputGB1_3629 YP_001669856 167034625 Pseudomonas putida GB-1

Enoyl-CoA reductase

Protein GenBank ID GI number Organism mecr XP_642118 66816217 Dictyostelium discoideum AX4 NEMVEDRAFT_v1g228294 XP_001639469 156402181 Nematostella vectensis AaeL_AAEL003995 XP_001648220 157104018 Aedes aegypti

In addition to the above exemplary encoding nucleic acids, nucleic acids other than those within the MI-FAE cycle, MD-FAE and/or termination pathways of the invention also can be introduced into a host organism for further production of fatty alcohols, fatty aldehydes or fatty acids. For example, the Ralstonia eutropha BktB and PhbB genes catalyze the condensation of butyryl-CoA and acetyl-CoA to form β-keto-hexanoyl-CoA and the reduction of β-keto-hexanoyl-CoA to 3-hydroxy-hexanoyl-CoA (Fukui et al., Biomacromolecules 3:618-624 (2002)). To improve the production of fatty alcohols, exogenous DNA sequences encoding for these specific enzymes can be expressed in the production host of interest. Furthermore, the above described enzymes can be subjected to directed evolution to generate improved versions of these enzymes with high activity and high substrate specificity. A similar approach also can be utilized with any or all other enzymatic steps in the fatty alcohol, fatty aldehyde or fatty acid producing pathways of the invention to, for example, improve enzymatic activity and/or specificity and/or to generate a fatty alcohol, a fatty aldehyde or a fatty acid of a predetermined chain length or lengths.

Example V Pathways for Producing Cytosolic Acetyl-CoA from Cytosolic Pyruvate

The following example describes exemplary pathways for the conversion of cytosolic pyruvate and threonine to cytosolic acetyl-CoA, as shown in FIG. 3.

Pathways for the conversion of cytosolic pyruvate and threonine to cytosolic acetyl-CoA could enable deployment of a cytosolic fatty alcohol, fatty aldehyde or fatty acid production pathway that originates from acetyl-CoA. Several pathways for converting cytosolic pyruvate to cytosolic acetyl-CoA are shown in FIG. 3. Direct conversion of pyruvate to acetyl-CoA can be catalyzed by pyruvate dehydrogenase, pyruvate formate lyase, pyruvate:NAD(P) oxidoreductase or pyruvate:ferredoxin oxidoreductase. If a pyruvate formate lyase is utilized, the formate byproduct can be further converted to CO2 by formate dehydrogenase or formate hydrogen lyase.

Indirect conversion of pyruvate to acetyl-CoA can proceed through several alternate routes. Pyruvate can be converted to acetaldehyde by a pyruvate decarboxylase. Acetaldehyde can then converted to acetyl-CoA by an acylating (CoA-dependent) acetaldehyde dehydrogenase. Alternately, acetaldehyde generated by pyruvate decarboxylase can be converted to acetyl-CoA by the “PDH bypass” pathway. In this pathway, acetaldehyde is oxidized by acetaldehyde dehydrogenase to acetate, which is then converted to acetyl-CoA by a CoA ligase, synthetase or transferase. In another embodiment, the acetate intermediate is converted by an acetate kinase to acetyl-phosphate that is then converted to acetyl-CoA by a phosphotransacetylase. In yet another embodiment, pyruvate is directly converted to acetyl-phosphate by a pyruvate oxidase (acetyl-phosphate forming). Conversion of pyruvate to acetate is also catalyzed by acetate-forming pyruvate oxidase.

Cytosolic acetyl-CoA can also be synthesized from threonine by expressing a native or heterologous threonine aldolase (FIG. 6J) (van Maris et al, AEM 69:2094-9 (2003)). Threonine aldolase converts threonine into acetaldehyde and glycine. The acetaldehyde product is subsequently converted to acetyl-CoA by various pathways described above.

Gene candidates for the acetyl-CoA forming enzymes shown in FIG. 3 are described below.

Pyruvate oxidase (acetate-forming) (FIG. 3A) or pyruvate:quinone oxidoreductase (PQO) can catalyze the oxidative decarboxylation of pyruvate into acetate, using ubiquione (EC 1.2.5.1) or quinone (EC 1.2.2.1) as an electron acceptor. The E. coli enzyme, PoxB, is localized on the inner membrane (Abdel-Hamid et al., Microbiol 147:1483-98 (2001)). The enzyme has thiamin pyrophosphate and flavin adenine dinucleotide (FAD) cofactors (Koland and Gennis, Biochemistry 21:4438-4442 (1982)); O'Brien et al., Biochemistry 16:3105-3109 (1977); O'Brien and Gennis, J. Biol. Chem. 255:3302-3307 (1980)). PoxB has similarity to pyruvate decarboxylase of S. cerevisiae and Zymomonas mobilis. The pqo transcript of Corynebacterium glutamicum encodes a quinone-dependent and acetate-forming pyruvate oxidoreductase (Schreiner et al., J Bacteriol 188:1341-50 (2006)). Similar enzymes can be inferred by sequence homology.

Protein GenBank ID GI Number Organism poxB NP_415392.1 16128839 Escherichia coli pqo YP_226851.1 62391449 Corynebacterium glutamicum poxB YP_309835.1 74311416 Shigella sonnei poxB ZP_03065403.1 194433121 Shigella dysenteriae

The acylation of acetate to acetyl-CoA (FIG. 3B) can be catalyzed by enzymes with acetyl-CoA synthetase, ligase or transferase activity. Two enzymes that can catalyze this reaction are AMP-forming acetyl-CoA synthetase or ligase (EC 6.2.1.1) and ADP-forming acetyl-CoA synthetase (EC 6.2.1.13). AMP-forming acetyl-CoA synthetase (ACS) is the predominant enzyme for activation of acetate to acetyl-CoA. Exemplary ACS enzymes are found in E. coli (Brown et al., J Gen. Microbiol. 102:327-336 (1977)), Ralstonia eutropha (Priefert and Steinbuchel, J. Bacteriol. 174:6590-6599 (1992)), Methanothermobacter thermautotrophicus (Ingram-Smith and Smith, Archaea 2:95-107 (2007)), Salmonella enterica (Gulick et al., Biochemistry 42:2866-2873 (2003)) and Saccharomyces cerevisiae (Jogl and Tong, Biochemistry 43:1425-1431 (2004)). ADP-forming acetyl-CoA synthetases are reversible enzymes with a generally broad substrate range (Musfeldt and Schonheit, J. Bacteriol. 184:636-644 (2002)). Two isozymes of ADP-forming acetyl-CoA synthetases are encoded in the Archaeoglobus fulgidus genome by are encoded by AF1211 and AF1983 (Musfeldt and Schonheit, supra (2002)). The enzyme from Haloarcula marismortui (annotated as a succinyl-CoA synthetase) also accepts acetate as a substrate and reversibility of the enzyme was demonstrated (Brasen and Schonheit, Arch. Microbiol. 182:277-287 (2004)). The ACD encoded by PAE3250 from hyperthermophilic crenarchaeon Pyrobaculum aerophilum showed the broadest substrate range of all characterized ACDs, reacting with acetate, isobutyryl-CoA (preferred substrate) and phenylacetyl-CoA (Brasen and Schonheit, supra (2004)). Directed evolution or engineering can be used to modify this enzyme to operate at the physiological temperature of the host organism. The enzymes from A. fulgidus, H marismortui and P. aerophilum have all been cloned, functionally expressed, and characterized in E. coli (Brasen and Schonheit, supra (2004); Musfeldt and Schonheit, supra (2002)). Additional candidates include the succinyl-CoA synthetase encoded by sucCD in E. coli (Buck et al., Biochemistry 24:6245-6252 (1985)) and the acyl-CoA ligase from Pseudomonas putida (Fernandez-Valverde et al., Appl. Environ. Microbiol. 59:1149-1154 (1993)). The aforementioned proteins are shown below.

Protein GenBank ID GI Number Organism acs AAC77039.1 1790505 Escherichia coli acoE AAA21945.1 141890 Ralstonia eutropha acs1 ABC87079.1 86169671 Methanothermobacter thermautotrophicus acs1 AAL23099.1 16422835 Salmonella enterica ACS1 Q01574.2 257050994 Saccharomyces cerevisiae AF1211 NP_070039.1 11498810 Archaeoglobus fulgidus AF1983 NP_070807.1 11499565 Archaeoglobus fulgidus scs YP_135572.1 55377722 Haloarcula marismortui PAE3250 NP_560604.1 18313937 Pyrobaculum aerophilum str. IM2 sucC NP_415256.1 16128703 Escherichia coli sucD AAC73823.1 1786949 Escherichia coli paaF AAC24333.2 22711873 Pseudomonas putida

The acylation of acetate to acetyl-CoA can also be catalyzed by CoA transferase enzymes (FIG. 3B). Numerous enzymes employ acetate as the CoA acceptor, resulting in the formation of acetyl-CoA. An exemplary CoA transferase is acetoacetyl-CoA transferase, encoded by the E. coli atoA (alpha subunit) and atoD (beta subunit) genes (Korolev et al., Acta Crystallogr. D. Biol. Crystallogr. 58:2116-2121 (2002); Vanderwinkel et al., 33:902-908 (1968)). This enzyme has a broad substrate range (Sramek et al., Arch Biochem Biophys 171:14-26 (1975)) and has been shown to transfer the CoA moiety to acetate from a variety of branched and linear acyl-CoA substrates, including isobutyrate (Matthies et al., Appl Environ. Microbiol 58:1435-1439 (1992)), valerate (Vanderwinkel et al., Biochem. Biophys. Res. Commun. 33:902-908 (1968)) and butanoate (Vanderwinkel et al., Biochem. Biophys. Res. Commun. 33:902-908 (1968)) Similar enzymes exist in Corynebacterium glutamicum ATCC 13032 (Duncan et al., 68:5186-5190 (2002)), Clostridium acetobutylicum (Cary et al., Appl Environ Microbiol 56:1576-1583 (1990); Wiesenborn et al., Appl Environ Microbiol 55:323-329 (1989)), and Clostridium saccharoperbutylacetonicum (Kosaka et al., Biosci. Biotechnol Biochem. 71:58-68 (2007)).

Gene GI # Accession No. Organism atoA 2492994 P76459.1 Escherichia coli atoD 2492990 P76458.1 Escherichia coli actA 62391407 YP_226809.1 Corynebacterium glutamicum cg0592 62389399 YP_224801.1 Corynebacterium glutamicum ctfA 15004866 NP_149326.1 Clostridium acetobutylicum ctfB 15004867 NP_149327.1 Clostridium acetobutylicum ctfA 31075384 AAP42564.1 Clostridium saccharoperbutylacetonicum ctfB 31075385 AAP42565.1 Clostridium saccharoperbutylacetonicum

Acetate kinase (EC 2.7.2.1) can catalyzes the reversible ATP-dependent phosphorylation of acetate to acetylphosphate (FIG. 3C). Exemplary acetate kinase enzymes have been characterized in many organisms including E. coli, Clostridium acetobutylicum and Methanosarcina thermophila (Ingram-Smith et al., J. Bacteriol. 187:2386-2394 (2005); Fox and Roseman, J. Biol. Chem. 261:13487-13497 (1986); Winzer et al., Microbiology 143 (Pt 10):3279-3286 (1997)). Acetate kinase activity has also been demonstrated in the gene product of E. coli purT (Marolewski et al., Biochemistry 33:2531-2537 (1994). Some butyrate kinase enzymes (EC 2.7.2.7), for example buk1 and buk2 from Clostridium acetobutylicum, also accept acetate as a substrate (Hartmanis, M. G., J. Biol. Chem. 262:617-621 (1987)). Homologs exist in several other organisms including Salmonella enterica and Chlamydomonas reinhardtii.

Protein GenBank ID GI Number Organism ackA NP_416799.1 16130231 Escherichia coli Ack AAB18301.1 1491790 Clostridium acetobutylicum Ack AAA72042.1 349834 Methanosarcina thermophila purT AAC74919.1 1788155 Escherichia coli buk1 NP_349675 15896326 Clostridium acetobutylicum buk2 Q97II1 20137415 Clostridium acetobutylicum ackA NP_461279.1 16765664 Salmonella typhimurium ACK1 XP_001694505.1 159472745 Chlamydomonas reinhardtii ACK2 XP_001691682.1 159466992 Chlamydomonas reinhardtii

The formation of acetyl-CoA from acetyl-phosphate can be catalyzed by phosphotransacetylase (EC 2.3.1.8) (FIG. 3D). The pta gene from E. coli encodes an enzyme that reversibly converts acetyl-CoA into acetyl-phosphate (Suzuki, T., Biochim. Biophys. Acta 191:559-569 (969)). Additional acetyltransferase enzymes have been characterized in Bacillus subtilis (Rado and Hoch, Biochim. Biophys. Acta 321:114-125 (1973), Clostridium kluyveri (Stadtman, E., Methods Enzymol. 1:5896-599 (1955), and Thermotoga maritima (Bock et al., J. Bacteriol. 181:1861-1867 (1999)). This reaction can also be catalyzed by some phosphotranbutyrylase enzymes (EC 2.3.1.19), including the ptb gene products from Clostridium acetobutylicum (Wiesenbom et al., App. Environ. Microbiol. 55:317-322 (1989); Walter et al., Gene 134:107-111 (1993)). Additional ptb genes are found in butyrate-producing bacterium L2-50 (Louis et al., J. Bacteriol. 186:2099-2106 (2004) and Bacillus megaterium (Vazquez et al., Curr. Microbiol. 42:345-349 (2001). Homologs to the E. coli pta gene exist in several other organisms including Salmonella enterica and Chlamydomonas reinhardtii.

Protein GenBank ID GI Number Organism Pta NP_416800.1 71152910 Escherichia coli Pta P39646 730415 Bacillus subtilis Pta A5N801 146346896 Clostridium kluyveri Pta Q9X0L4 6685776 Thermotoga maritime Ptb NP_349676 34540484 Clostridium acetobutylicum Ptb AAR19757.1 38425288 butyrate-producing bacterium L2-50 Ptb CAC07932.1 10046659 Bacillus megaterium Pta NP_461280.1 16765665 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 PAT2 XP_001694504.1 159472743 Chlamydomonas reinhardtii PAT1 XP_001691787.1 159467202 Chlamydomonas reinhardtii

Pyruvate decarboxylase (PDC) is a key enzyme in alcoholic fermentation, catalyzing the decarboxylation of pyruvate to acetaldehyde (FIG. 3E). The PDC1 enzyme from Saccharomyces cerevisiae has been extensively studied (Killenberg-Jabs et al., Eur. J. Biochem. 268:1698-1704 (2001); Li et al., Biochemistry. 38:10004-10012 (1999); ter Schure et al., Appl. Environ. Microbiol. 64:1303-1307 (1998)). Other well-characterized PDC enzymes are found in Zymomonas mobilus (Siegert et al., Protein Eng Des Sel 18:345-357 (2005)), Acetobacter pasteurians (Chandra et al., 176:443-451(2001)) and Kluyveromyces lactis (Krieger et al., 269:3256-3263 (2002)). The PDC1 and PDC5 enzymes of Saccharomyces cerevisiae are subject to positive transcriptional regulation by PDC2 (Hohmann et al, Mol Gen Genet 241:657-66 (1993)). Pyruvate decarboxylase activity is also possessed by a protein encoded by CTRG_03826 (GI:255729208) in Candida tropicalis, PDC1 (GI number: 1226007) in Kluyveromyces lactis, YALI0D10131g (GI:50550349) in Yarrowia lipolytica, PAS_chr_30188 (GI:254570575) in Pichia pastoris, pyruvate decarboxylase (GI: GI:159883897) in Schizosaccharomyces pombe, ANI_1_1024084 (GI:145241548), ANI_1_796114 (GI:317034487), ANI_1_936024 (GI:317026934) and ANI_1_2276014 (GI:317025935) in Aspergillus niger.

Protein GenBank ID GI Number Organism pdc P06672.1 118391 Zymomonas mobilis pdc1 P06169 30923172 Saccharomyces cerevisiae Pdc2 NP_010366.1 6320286 Saccharomyces cerevisiae Pdc5 NP_013235.1 6323163 Saccharomyces cerevisiae CTRG_03826 XP_002549529 255729208 Candida tropicalis, CU329670.1:585597.587312 CAA90807 159883897 Schizosaccharomyces pombe YALI0D10131g XP_502647 50550349 Yarrowia lipolytica PAS_chr3_0188 XP_002492397 254570575 Pichia pastoris pdc Q8L388 20385191 Acetobacter pasteurians pdc1 Q12629 52788279 Kluyveromyces lactis ANI_1_1024084 XP_001393420 145241548 Aspergillus niger ANI_1_796114 XP_001399817 317026934 Aspergillus niger ANI_1_936024 XP_001396467 317034487 Aspergillus niger ANI_1_2276014 XP_001388598 317025935 Aspergillus niger

Aldehyde dehydrogenase enzymes in EC class 1.2.1 catalyze the oxidation of acetaldehyde to acetate (FIG. 3F). Exemplary genes encoding this activity were described above. The oxidation of acetaldehyde to acetate can also be catalyzed by an aldehyde oxidase with acetaldehyde oxidase activity. Such enzymes can convert acetaldehyde, water and O₂ to acetate and hydrogen peroxide. Exemplary aldehyde oxidase enzymes that have been shown to catalyze this transformation can be found in Bos taurus and Mus musculus (Garattini et al., Cell Mol Life Sci 65:109-48 (2008); Cabre et al., Biochem Soc Trans 15:882-3 (1987)). Additional aldehyde oxidase gene candidates include the two flavin- and molybdenum-containing aldehyde oxidases of Zea mays, encoded by zmAO-1 and zmAO-2 (Sekimoto et al., J Biol Chem 272:15280-85 (1997)).

GenBank Gene Accession No. GI No. Organism zmAO-1 NP_001105308.1 162458742 Zea mays zmAO-2 BAA23227.1 2589164 Zea mays Aox1 O54754.2 20978408 Mus musculus XDH DAA24801.1 296482686 Bos taurus

Pyruvate oxidase (acetyl-phosphate forming) can catalyze the conversion of pyruvate, oxygen and phosphate to acetyl-phosphate and hydrogen peroxide (FIG. 3G). This type of pyruvate oxidase is soluble and requires the cofactors thiamin diphosphate and flavin adenine dinucleotide (FAD). Acetyl-phosphate forming pyruvate oxidase enzymes can be found in lactic acid bacteria Lactobacillus delbrueckii and Lactobacillus plantarum (Lorquet et al., J Bacteriol 186:3749-3759 (2004); Hager et al., Fed Proc 13:734-38 (1954)). A crystal structure of the L. plantarum enzyme has been solved (Muller et al., (1994)). In Streptococcus sanguinis and Streptococcus pneumonia, acetyl-phosphate forming pyruvate oxidase enzymes are encoded by the spxB gene (Spellerberg et al., Mol Micro 19:803-13 (1996); Ramos-Montanez et al., Mol Micro 67:729-46 (2008)). The SpxR was shown to positively regulate the transcription of spxB in S. pneumoniae (Ramos-Montanez et al., supra). A similar regulator in S. sanguinis was identified by sequence homology. Introduction or modification of catalase activity can reduce accumulation of the hydrogen peroxide product.

GenBank Gene Accession No. GI No. Organism poxB NP_786788.1 28379896 Lactobacillus plantarum spxB L39074.1 1161269 Streptococcus pneumoniae Spd_0969 YP_816445.1 116517139 Streptococcus pneumoniae (spxR) spxB ZP_07887723.1 315612812 Streptococcus sanguinis spxR ZP_07887944.1 315613033 Streptococcus sanguinis GI:

The pyruvate dehydrogenase (PDH) complex catalyzes the conversion of pyruvate to acetyl-CoA (FIG. 3H). The E. coli PDH complex is encoded by the genes aceEF and lpdA Enzyme engineering efforts have improved the E. coli PDH enzyme activity under anaerobic conditions (Kim et al., J. Bacteriol. 190:3851-3858 (2008); Kim et al., Appl. Environ. Microbiol. 73:1766-1771 (2007); Zhou et al., Biotechnol. Lett. 30:335-342 (2008)). In contrast to the E. coli PDH, the B. subtilis complex is active and required for growth under anaerobic conditions (Nakano et al., 179:6749-6755 (1997)). The Klebsiella pneumoniae PDH, characterized during growth on glycerol, is also active under anaerobic conditions (Menzel et al., 56:135-142 (1997)). Crystal structures of the enzyme complex from bovine kidney (Zhou et al., 98:14802-14807 (2001)) and the E2 catalytic domain from Azotobacter vinelandii are available (Mattevi et al., Science. 255:1544-1550 (1992)). Some mammalian PDH enzymes complexes can react on alternate substrates such as 2-oxobutanoate. Comparative kinetics of Rattus norvegicus PDH and BCKAD indicate that BCKAD has higher activity on 2-oxobutanoate as a substrate (Paxton et al., Biochem. J. 234:295-303 (1986)). The S. cerevisiae PDH complex canconsist of an E2 (LAT1) core that binds E1 (PDA1, PDB1), E3 (LPD1), and Protein X (PDX1) components (Pronk et al., Yeast 12:1607-1633 (1996)). The PDH complex of S. cerevisiae is regulated by phosphorylation of E1 involving PKP1 (PDH kinase I), PTC5 (PDH phosphatase I), PKP2 and PTC6. Modification of these regulators may also enhance PDH activity. Coexpression of lipoyl ligase (LplA of E. coli and AIM422 in S. cerevisiae) with PDH in the cytosol may be necessary for activating the PDH enzyme complex. Increasing the supply of cytosolic lipoate, either by modifying a metabolic pathway or media supplementation with lipoate, may also improve PDH activity.

Gene Accession No. GI Number Organism aceE NP_414656.1 16128107 Escherichia coli aceF NP_414657.1 16128108 Escherichia coli lpd NP_414658.1 16128109 Escherichia coli lplA NP_418803.1 16132203 Escherichia coli pdhA P21881.1 3123238 Bacillus subtilis pdhB P21882.1 129068 Bacillus subtilis pdhC P21883.2 129054 Bacillus subtilis pdhD P21880.1 118672 Bacillus subtilis aceE YP_001333808.1 152968699 Klebsiella pneumoniae aceF YP_001333809.1 152968700 Klebsiella pneumoniae lpdA YP_001333810.1 152968701 Klebsiella pneumoniae Pdha1 NP_001004072.2 124430510 Rattus norvegicus Pdha2 NP_446446.1 16758900 Rattus norvegicus Dlat NP_112287.1 78365255 Rattus norvegicus Dld NP_955417.1 40786469 Rattus norvegicus LAT1 NP_014328 6324258 Saccharomyces cerevisiae PDA1 NP_011105 37362644 Saccharomyces cerevisiae PDB1 NP_009780 6319698 Saccharomyces cerevisiae LPD1 NP_116635 14318501 Saccharomyces cerevisiae PDX1 NP_011709 6321632 Saccharomyces cerevisiae AIM22 NP_012489.2 83578101 Saccharomyces cerevisiae

As an alternative to the large multienzyme PDH complexes described above, some organisms utilize enzymes in the 2-ketoacid oxidoreductase family (OFOR) to catalyze acylating oxidative decarboxylation of 2-keto-acids. Unlike the PDH complexes, PFOR enzymes contain iron-sulfur clusters, utilize different cofactors and use ferredoxin or flavodixin as electron acceptors in lieu of NAD(P)H. Pyruvate ferredoxin oxidoreductase (PFOR) can catalyze the oxidation of pyruvate to form acetyl-CoA (FIG. 3H). The PFOR from Desulfovibrio africanus has been cloned and expressed in E. coli resulting in an active recombinant enzyme that was stable for several days in the presence of oxygen (Pieulle et al., J Bacteriol. 179:5684-5692 (1997)). Oxygen stability is relatively uncommon in PFORs and is believed to be conferred by a 60 residue extension in the polypeptide chain of the D. africanus enzyme. The M. thermoacetica PFOR is also well characterized (Menon et al., Biochemistry 36:8484-8494 (1997)) and was even shown to have high activity in the direction of pyruvate synthesis during autotrophic growth (Furdui et al., J Biol Chem. 275:28494-28499 (2000)). Further, E. coli possesses an uncharacterized open reading frame, ydbK, that encodes a protein that is 51% identical to the M. thermoacetica PFOR. Evidence for pyruvate oxidoreductase activity in E. coli has been described (Blaschkowski et al., Eur. J Biochem. 123:563-569 (1982)). Several additional PFOR enzymes are described in Ragsdale, Chem. Rev. 103:2333-2346 (2003). Finally, flavodoxin reductases (e.g., fqrB from Helicobacter pylori or Campylobacter jejuni (St Maurice et al., J. Bacteriol. 189:4764-4773 (2007))) or Rnf-type proteins (Seedorf et al., Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133 (2008); Herrmann et al., J. Bacteriol. 190:784-791 (2008)) provide a means to generate NADH or NADPH from the reduced ferredoxin generated by PFOR. These proteins are identified below.

Protein GenBank ID GI Number Organism Por CAA70873.1 1770208 Desulfovibrio africanus Por YP_428946.1 83588937 Moorella thermoacetica ydbK NP_415896.1 16129339 Escherichia coli fqrB NP_207955.1 15645778 Helicobacter pylori fqrB YP_001482096.1 157414840 Campylobacter jejuni RnfC EDK33306.1 146346770 Clostridium kluyveri RnfD EDK33307.1 146346771 Clostridium kluyveri RnfG EDK33308.1 146346772 Clostridium kluyveri RnfE EDK33309.1 146346773 Clostridium kluyveri RnfA EDK33310.1 146346774 Clostridium kluyveri RnfB EDK33311.1 146346775 Clostridium kluyveri

Pyruvate formate-lyase (PFL, EC 2.3.1.54) (FIG. 3H), encoded by pflB in E. coli, can convert pyruvate into acetyl-CoA and formate. The activity of PFL can be enhanced by an activating enzyme encoded by pflA (Knappe et al., Proc. Natl. Acad. Sci U.S.A 81:1332-1335 (1984); Wong et al., Biochemistry 32:14102-14110 (1993)). Keto-acid formate-lyase (EC 2.3.1.-), also known as 2-ketobutyrate formate-lyase (KFL) and pyruvate formate-lyase 4, is the gene product of tdcE in E. coll. This enzyme catalyzes the conversion of 2-ketobutyrate to propionyl-CoA and formate during anaerobic threonine degradation, and can also substitute for pyruvate formate-lyase in anaerobic catabolism (Simanshu et al., J Biosci. 32:1195-1206 (2007)). The enzyme is oxygen-sensitive and, like PflB, can require post-translational modification by PFL-AE to activate a glycyl radical in the active site (Hesslinger et al., Mol. Microbiol 27:477-492 (1998)). A pyruvate formate-lyase from Archaeoglobus fulgidus encoded by pflD has been cloned, expressed in E. coli and characterized (Lehtio et al., Protein Eng Des Sel 17:545-552 (2004)). The crystal structures of the A. fulgidus and E. coli enzymes have been resolved (Lehtio et al., J Mol. Biol. 357:221-235 (2006); Leppanen et al., Structure. 7:733-744 (1999)). Additional PFL and PFL-AE candidates are found in Lactococcus lactis (Melchiorsen et al., Appl Microbiol Biotechnol 58:338-344 (2002)), and Streptococcus mutans (Takahashi-Abbe et al., Oral. Microbiol Immunol. 18:293-297 (2003)), Chlamydomonas reinhardtii (Hemschemeier et al., Eukaryot. Cell 7:518-526 (2008b); Atteia et al., J. Biol. Chem. 281:9909-9918 (2006)) and Clostridium pasteurianum (Weidner et al., J Bacteriol. 178:2440-2444 (1996)).

Protein GenBank ID GI Number Organism pflB NP_415423 16128870 Escherichia coli pflA NP_415422.1 16128869 Escherichia coli tdcE AAT48170.1 48994926 Escherichia coli pflD NP_070278.1 11499044 Archaeoglobus fulgidus pfl CAA03993 2407931 Lactococcus lactis pfl BAA09085 1129082 Streptococcus mutans PFL1 XP_001689719.1 159462978 Chlamydomonas reinhardtii pflA1 XP_001700657.1 159485246 Chlamydomonas reinhardtii pfl Q46266.1 2500058 Clostridium pasteurianum act CAA63749.1 1072362 Clostridium pasteurianum

If a pyruvate formate lyase is utilized to convert pyruvate to acetyl-CoA, coexpression of a formate dehydrogenase or formate hydrogen lyase enzyme will convene formate to carbon dioxide. Formate dehydrogenase (FDH) catalyzes the reversible transfer of electrons from formate to an acceptor. Enzymes with FDH activity utilize various electron carriers such as, for example, NADH (EC 1.2.1.2), NADPH (EC 1.2.1.43), quinols (EC 1.1.5.6), cytochromes (EC 1.2.2.3) and hydrogenases (EC 1.1.99.33). FDH enzymes have been characterized from Moorella thermoacetica (Andreesen and Ljungdahl, J Bacteriol 116:867-873 (1973); Li et al., J Bacteriol 92:405-412 (1966); Yamamoto et al., J Biol Chem. 258:1826-1832 (1983). The loci, Moth_2312 is responsible for encoding the alpha subunit of formate dehydrogenase while the beta subunit is encoded by Moth_2314 (Pierce et al., Environ Microbiol (2008)). Another set of genes encoding formate dehydrogenase activity with a propensity for CO₂ reduction is encoded by Sfum_2703 through Sfum_2706 in Syntrophobacter fumaroxidans (de Bok et al., Eur J Biochem. 270:2476-2485 (2003)); Reda et al., PNAS 105:10654-10658 (2008)). A similar set of genes presumed to carry out the same function are encoded by CHY_0731, CHY_0732, and CHY_0733 in C. hydrogenoformans (Wu et al., PLoS Genet 1:e65 (2005)). Formate dehydrogenases are also found many additional organisms including C. carboxidivorans P7, Bacillus methanolicus, Burkholderia stabilis, Moorella thermoacetica ATCC 39073, Candida boidinii, Candida methylica, and Saccharomyces cerevisiae S288c.

Protein GenBank ID GI Number Organism Moth_2312 YP_431142 148283121 Moorella thermoacetica Moth_2314 YP_431144 83591135 Moorella thermoacetica Sfum_2703 YP_846816.1 116750129 Syntrophobacter fumaroxidans Sfum_2704 YP_846817.1 116750130 Syntrophobacter fumaroxidans Sfum_2705 YP_846818.1 116750131 Syntrophobacter fumaroxidans Sfum_2706 YP_846819.1 116750132 Syntrophobacter fumaroxidans CHY_0731 YP_359585.1 78044572 Carboxydothermus hydrogenoformans CHY_0732 YP_359586.1 78044500 Carboxydothermus hydrogenoformans CHY_0733 YP_359587.1 78044647 Carboxydothermus hydrogenoformans CcarbDRAFT_0901 ZP_05390901.1 255523938 Clostridium carboxidivorans P7 CcarbDRAFT_4380 ZP_05394380.1 255527512 Clostridium carboxidivorans P7 fdhA, MGA3_06625 EIJ82879.1 387590560 Bacillus methanolicus MGA3 fdhA, PB1_11719 ZP_10131761.1 387929084 Bacillus methanolicus PB1 fdhD, MGA3_06630 EIJ82880.1 387590561 Bacillus methanolicus MGA3 fdhD, PB1_11724 ZP_10131762.1 387929085 Bacillus methanolicus PB1 fdh ACF35003. 194220249 Burkholderia stabilis FDH1 AAC49766.1 2276465 Candida boidinii fdh CAA57036.1 1181204 Candida methylica FDH2 P0CF35.1 294956522 Saccharomyces cerevisiae S288c FDH1 NP_015033.1 6324964 Saccharomyces cerevisiae S288c

Alternately, a formate hydrogen lyase enzyme can be employed to convert formate to carbon dioxide and hydrogen. An exemplary formate hydrogen lyase enzyme can be found in Escherichia coli. The E. coli formate hydrogen lyase consists of hydrogenase 3 and formate dehydrogenase-H (Maeda et al., Appl Microbiol Biotechnol 77:879-890 (2007)). It is activated by the gene product of fhlA. (Maeda et al., Appl Microbiol Biotechnol 77:879-890 (2007)). The addition of the trace elements, selenium, nickel and molybdenum, to a fermentation broth has been shown to enhance formate hydrogen lyase activity (Soini et al., Microb. Cell Fact. 7:26 (2008)). Various hydrogenase 3, formate dehydrogenase and transcriptional activator genes are shown below. A formate hydrogen lyase enzyme also exists in the hyperthermophilic archaeon, Thermococcus litoralis (Takacs et al., BMC. Microbiol 8:88 (2008)). Additional formate hydrogen lyase systems have been found in Salmonella typhimurium, Klebsiella pneumoniae, Rhodospirillum rubrum, Methanobacterium formicicum (Vardar-Schara et al., Microbial Biotechnology 1:107-125 (2008)).

Protein GenBank ID GI number Organism hycA NP_417205 16130632 Escherichia coli K-12 MG1655 hycB NP_417204 16130631 Escherichia coli K-12 MG1655 hycC NP_417203 16130630 Escherichia coli K-12 MG1655 hycD NP_417202 16130629 Escherichia coli K-12 MG1655 hycE NP_417201 16130628 Escherichia coli K-12 MG1655 hycF NP_417200 16130627 Escherichia coli K-12 MG1655 hycG NP_417199 16130626 Escherichia coli K-12 MG1655 hycH NP_417198 16130625 Escherichia coli K-12 MG1655 hycI NP_417197 16130624 Escherichia coli K-12 MG1655 fdhF NP_418503 16131905 Escherichia coli K-12 MG1655 fhlA NP_417211 16130638 Escherichia coli K-12 MG1655 mhyC ABW05543 157954626 Thermococcus litoralis mhyD ABW05544 157954627 Thermococcus litoralis mhyE ABW05545 157954628 Thermococcus litoralis mhyF ABW05546 157954629 Thermococcus litoralis mhyG ABW05547 157954630 Thermococcus litoralis mhyH ABW05548 157954631 Thermococcus litoralis fdhA AAB94932 2746736 Thermococcus litoralis fdhB AAB94931 157954625 Thermococcus litoralis

Pyruvate:NADP oxidoreductase (PNO) catalyzes the conversion of pyruvate to acetyl-CoA. This enzyme is encoded by a single gene and the active enzyme is a homodimer, in contrast to the multi-subunit PDH enzyme complexes described above. The enzyme from Euglena gracilis is stabilized by its cofactor, thiamin pyrophosphate (Nakazawa et al, Arch Biochem Biophys 411:183-8 (2003)). The mitochondrial targeting sequence of this enzyme should be removed for expression in the cytosol. The PNO protein of E. gracilis protein and other NADP-dependant pyruvate:NADP+ oxidoreductase enzymes are listed in the table below.

Protein GenBank ID GI Number Organism PNO Q94IN5.1 33112418 Euglena gracilis cgd4_690 XP_625673.1 66356990 Cryptosporidium parvum Iowa II TPP_PFOR_PNO XP_002765111.11 294867463 Perkinsus marinus ATCC 50983

The NAD(P)⁺ dependent oxidation of acetaldehyde to acetyl-CoA (FIG. 3I) can be catalyzed by an acylating acetaldehyde dehydrogenase (EC 1.2.1.10). Acylating acetaldehyde dehydrogenase enzymes of E. coli are encoded by adhE, eutE, and mhpF (Ferrandez et al, J Bacteriol 179:2573-81 (1997)). The Pseudomonas sp. CF600 enzyme, encoded by dmpF, participates in meta-cleavage pathways and forms a complex with 4-hydroxy-2-oxovalerate aldolase (Shingler et al, J Bacteriol 174:711-24 (1992)). Solventogenic organisms such as Clostridium acetobutylicum encode bifunctional enzymes with alcohol dehydrogenase and acetaldehyde dehydrogenase activities. The bifunctional C. acetobutylicum enzymes are encoded by bdh I and adhE2 (Walter, et al., J. Bacteriol. 174:7149-7158 (1992); Fontaine et al., J. Bacteriol. 184:821-830 (2002)). Yet another candidate for acylating acetaldehyde dehydrogenase is the ald gene from Clostridium beijerinckii (Toth, Appl. Environ. Microbiol. 65:4973-4980 (1999). This gene is very similar to the eutE acetaldehyde dehydrogenase genes of Salmonella typhimurium and E. coli (Toth, Appl. Environ. Microbiol. 65:4973-4980 (1999).

Protein GenBank ID GI Number Organism adhE NP_415757.1 16129202 Escherichia coli mhpF NP_414885.1 16128336 Escherichia coli dmpF CAA43226.1 45683 Pseudomonas sp. CF600 adhE2 AAK09379.1 12958626 Clostridium acetobutylicum bdh I NP_349892.1 15896543 Clostridium acetobutylicum Ald AAT66436 49473535 Clostridium beijerinckii eutE NP_416950 16130380 Escherichia coli eutE AAA80209 687645 Salmonella typhimurium

Threonine aldolase (EC 4.1.2.5) catalyzes the cleavage of threonine to glycine and acetaldehyde (FIG. 3J). The Saccharomyces cerevisiae and Candida albicans enzymes are encoded by GLY1 (Liu et al, Eur J Biochem 245:289-93 (1997); McNeil et al, Yeast 16:167-75 (2000)). The ltaE and glyA gene products of E. coli also encode enzymes with this activity (Liu et al, Eur J Biochem 255:220-6 (1998)).

Protein GenBank ID GI Number Organism GLY1 NP_010868.1 6320789 Saccharomyces cerevisiae GLY1 AAB64198.1 2282060 Candida albicans ltaE AAC73957.1 1787095 Escherichia coli glyA AAC75604.1 1788902 Escherichia coli

Example VI Pathways for Producing Acetyl-CoA from PEP and Pyruvate

Pathways for the conversion of cytosolic phosphoenolpyruvate (PEP) and pyruvate to cytosolic acetyl-CoA can also enable deployment of a cytosolic fatty alcohol, fatty aldehyde or fatty acid production pathway from acetyl-CoA. FIG. 4 shows numerous pathways for converting PEP and pyruvate to acetyl-CoA.

The conversion of PEP to oxaloacetate is catalyzed in one, two or three enzymatic steps. Oxaloacetate is further converted to acetyl-CoA via malonate semialdehyde or malonyl-CoA intermediates. In one pathway, PEP carboxylase or PEP carboxykinase converts PEP to oxaloacetate (step A); oxaloacetate decarboxylase converts the oxaloacetate to malonate (step B); and malonate semialdehyde dehydrogenase (acetylating) converts the malonate semialdehyde to acetyl-CoA (step C). In another pathway pyruvate kinase or PEP phosphatase converts PEP to pyruvate (step N); pyruvate carboxylase converts the pyruvate to (step H); oxaloacetate decarboxylase converts the oxaloacetate to malonate (step B); and malonate semialdehyde dehydrogenase (acetylating) converts the malonate semialdehyde to acetyl-CoA (step C). In another pathway pyruvate kinase or PEP phosphatase converts PEP to pyruvate (step N); malic enzyme converts the pyruvate to malate (step L); malate dehydrogenase or oxidoreductase converts the malate to oxaloacetate (step M); oxaloacetate decarboxylase converts the oxaloacetate to malonate (step B); and malonate semialdehyde dehydrogenase (acetylating) converts the malonate semialdehyde to acetyl-CoA (step C). In another pathway, PEP carboxylase or PEP carboxykinase converts PEP to oxaloacetate (step A); oxaloacetate decarboxylase converts the oxaloacetate to malonate semialdehyde (step B); malonyl-CoA reductase converts the malonate semialdehyde to malonyl-CoA (step G); and malonyl-CoA decarboxylase converts the malonyl-CoA to acetyl-CoA (step (D). In another pathway, pyruvate kinase or PEP phosphatase converts PEP to pyruvate (step N); pyruvate carboxylase converts the pyruvate to oxaloacetate (step H); (oxaloacetate decarboxylase converts the oxaloacetate to malonate semialdehyde (step B); malonyl-CoA reductase converts the malonate semialdehyde to malonyl-CoA (step G); and malonyl-CoA decarboxylase converts the malonyl-CoA to acetyl-CoA (step (D). In another pathway, pyruvate kinase or PEP phosphatase converts PEP to pyruvate (step N); malic enzyme converts the pyruvate to malate (step L); malate dehydrogenase or oxidoreductase converts the malate to oxaloacetate (step M); oxaloacetate decarboxylase converts the oxaloacetate to malonate semialdehyde (step B); malonyl-CoA reductase converts the malonate semialdehyde to malonyl-CoA (step G); and malonyl-CoA decarboxylase converts the malonyl-CoA to acetyl-CoA (step (D). In another pathway, PEP carboxylase or PEP carboxykinase converts PEP to oxaloacetate (step A); oxaloacetate decarboxylase converts the oxaloacetate to malonate semialdehyde (step B); malonate semialdehyde dehydrogenase converts the malonate semialdehyde to malonate (step J); malonyl-CoA synthetase or transferase converts the malonate to malonyl-CoA (step K); and malonyl-CoA decarboxylase converts the malonyl-CoA to acetyl-CoA (step D). In another pathway, pyruvate kinase or PEP phosphatase converts PEP to pyruvate (step N); pyruvate carboxylase converts the pyruvate to oxaloacetate (step H); oxaloacetate decarboxylase converts the oxaloacetate to malonate semialdehyde (step B); malonate semialdehyde dehydrogenase converts the malonate semialdehyde to malonate (step J); malonyl-CoA synthetase or transferase converts the malonate to malonyl-CoA (step K); and malonyl-CoA decarboxylase converts the malonyl-CoA to acetyl-CoA (step D). In another pathway, pyruvate kinase or PEP phosphatase converts PEP to pyruvate (step N); malic enzyme converts the pyruvate to malate (step L); malate dehydrogenase or oxidoreductase converts the malate to oxaloacetate (step M); oxaloacetate decarboxylase converts the oxaloacetate to malonate semialdehyde (step B); malonate semialdehyde dehydrogenase converts the malonate semialdehyde to malonate (step J); malonyl-CoA synthetase or transferase converts the malonate to malonyl-CoA (step K); and malonyl-CoA decarboxylase converts the malonyl-CoA to acetyl-CoA (step D). In another pathway, PEP carboxylase or PEP carboxykinase converts PEP to oxaloacetate (step A); oxaloacetate dehydrogenase or oxaloacetate oxidoreductase converts the oxaloacetate to malonyl-CoA (step F); and malonyl-CoA decarboxylase converts the malonyl-CoA to acetyl-CoA (step D). In another pathway, pyruvate kinase or PEP phosphatase converts PEP to pyruvate (step N); pyruvate carboxylase converts the pyruvate to oxaloacetate (step H); oxaloacetate dehydrogenase or oxaloacetate oxidoreductase converts the oxaloacetate to malonyl-CoA (step F); and malonyl-CoA decarboxylase converts the malonyl-CoA to acetyl-CoA (step D). In another pathway, pyruvate kinase or PEP phosphatase converts PEP to pyruvate (step N); malic enzyme converts the pyruvate to malate (step L); malate dehydrogenase or oxidoreductase converts the malate to oxaloacetate (step M); oxaloacetate dehydrogenase or oxaloacetate oxidoreductase converts the oxaloacetate to malonyl-CoA (step F); and malonyl-CoA decarboxylase converts the malonyl-CoA to acetyl-CoA (step D).

Enzymes candidates for the reactions shown in FIG. 4 are described below.

1.1.n.a Oxidoreductase (alcohol to oxo) M 1.1.1.d Malic enzyme L 1.2.1.a Oxidoreductase (aldehyde to acid) J 1.2.1.b Oxidoreductase (acyl-CoA to aldehyde) G 1.2.1.f Oxidoreductase (decarboxylating acyl-CoA to C aldehyde) 2.7.2.a Kinase N 2.8.3.a CoA transferase K 3.1.3.a Phosphatase N 4.1.1.a Decarboxylase A, B, D 6.2.1.a CoA synthetase K 6.4.1.a Carboxylase D, H

Enzyme candidates for several enzymes in FIG. 4 have been described elsewhere herein. These include acetyl-CoA carboxylase, acetoacetyl-CoA synthase, acetoacetyl-CoA thiolase, malonyl-CoA reductase (also called malonate semialdehyde dehydrogenase (acylating), malate dehydrogenase.

1.1.n.a Oxidoreductase (Alcohol to Oxo)

Malate dehydrogenase or oxidoreductase catalyzes the oxidation of malate to oxaloacetate. Different carriers can act as electron acceptors for enzymes in this class. Malate dehydrogenase enzymes utilize NADP or NAD as electron acceptors. Malate dehydrogenase (Step M) enzyme candidates are described herein. Malate:quinone oxidoreductase enzymes (EC 1.1.5.4) are membrane-associated and utilize quinones, flavoproteins or vitamin K as electron acceptors. Malate:quinone oxidoreductase enzymes of E. coli, Helicobacter pylori and Pseudomonas syringae are encoded by mqo (Kather et al, J Bacteriol 182:3204-9 (2000); Mellgren et al, J Bacteriol 191:3132-42 (2009)). The Cgl2001 gene of C. gluamicum also encodes an MQO enzyme (Mitsuhashi et al, Biosci Biotechnol Biochem 70:2803-6 (2006)).

Protein GenBank ID GI Number Organism mqo NP_416714.1 16130147 Escherichia coli mqo NP_206886.1 15644716 Helicobacter pylori mqo NP_790970.1 28868351 Pseudomonas syringae Cgl2001 NP_601207.1 19553205 Corynebacterium glutamicum

1.1.1.d Malic Enzyme

Malic enzyme (malate dehydrogenase) catalyzes the reversible oxidative carboxylation of pyruvate to malate. E. coli encodes two malic enzymes, MaeA and MaeB (Takeo, J Biochem. 66:379-387 (1969)). Although malic enzyme is typically assumed to operate in the direction of pyruvate formation from malate, the NAD-dependent enzyme, encoded by maeA, has been demonstrated to operate in the carbon-fixing direction (Stols and Donnelly, Appl. Environ. Microbiol. 63(7) 2695-2701 (1997)). A similar observation was made upon overexpressing the malic enzyme from Ascaris suum in E. coli (Stols et al., Appl. Biochem. Biotechnol. 63-65(1), 153-158 (1997)). The second E. coli malic enzyme, encoded by maeB, is NADP-dependent and also decarboxylates oxaloacetate and other alpha-keto acids (Iwakura et al., J. Biochem. 85(5):1355-65 (1979)). Another suitable enzyme candidate is me1 from Zea mays (Furumoto et al, Plant Cell Physiol 41:1200-1209 (2000)).

Protein GenBank ID GI Number Organism maeA NP_415996 90111281 Escherichia coli maeB NP_416958 16130388 Escherichia coli NAD-ME P27443 126732 Ascaris suum Me1 P16243.1 126737 Zea mays

1.2.1.a Oxidoreductase (Aldehyde to Acid)

The oxidation of malonate semialdehyde to malonate is catalyzed by malonate semialdehyde dehydrogenase (EC 1.2.1.15). This enzyme was characterized in Pseudomonas aeruginosa (Nakamura et al, Biochim Biophys Acta 50:147-52 (1961)). The NADP and NAD-dependent succinate semialdehyde dehydrogenase enzymes of Euglena gracilas accept malonate semialdehyde as substrates (Tokunaga et al, Biochem Biophys Act 429:55-62 (1976)). Genes encoding these enzymes has not been identified to date. Aldehyde dehydrogenase enzymes from eukoryotic organisms such as S. cerevisiae, C. albicans, Y. lipolytica and A. niger typically have broad substrate specificity and are suitable candidates. These enzymes and other acid forming aldehyde dehydrogenase and aldehyde oxidase enzymes are described earlier and listed in Tables 9 and 30. Additional MSA dehydrogenase enzyme candidates include NAD(P)+-dependent aldehyde dehydrogenase enzymes (EC 1.2.1.3). Two aldehyde dehydrogenases found in human liver, ALDH-1 and ALDH-2, have broad substrate ranges for a variety of aliphatic, aromatic and polycyclic aldehydes (Klyosov, Biochemistry 35:4457-4467 (1996a)). Active ALDH-2 has been efficiently expressed in E. coli using the GroEL proteins as chaperonins (Lee et al., Biochem. Biophys. Res. Commun. 298:216-224 (2002)). The rat mitochondrial aldehyde dehydrogenase also has a broad substrate range (Siew et al., Arch. Biochem. Biophys. 176:638-649 (1976)). The E. coli genes astD and aldH encode NAD+-dependent aldehyde dehydrogenases. AstD is active on succinic semialdehyde (Kuznetsova et al., FEMS Microbiol Rev 29:263-279 (2005)) and aldH is active on a broad range of aromatic and aliphatic substrates (Jo et al, Appl Microbiol Biotechnol 81:51-60 (2008)).

Gene GenBank Accession No. GI No. Organism astD P76217.1 3913108 Escherichia coli aldH AAC74382.1 1787558 Escherichia coli ALDH-2 P05091.2 118504 Homo sapiens ALDH-2 NP_115792.1 14192933 Rattus norvegicus

1.2.1.1 Oxidoreductase (Decarboxylating Acyl-CoA to Aldehyde)

Malonate semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) catalyzes the oxidative decarboxylation of malonate semialdehyde to acetyl-CoA. Exemplary enzymes are encoded by ddcC of Halomonas sp. HTNK1 (Todd et al, Environ Microbiol 12:237-43 (2010)) and IolA of Lactobacillus casei (Yebra et al, AEM 73:3850-8 (2007)). The DdcC enzyme has homologs in A. niger and C. albicans, shown in the table below. The malonate semialdehyde dehydrogenase enzyme in Rattus norvegicus, Mmsdh, also converts malonate semialdehyde to acetyl-CoA (U.S. Pat. No. 8,048,624). A malonate semialdehyde dehydrogenase (acetylating) enzyme has also been characterized in Pseudomonas fluorescens, although the gene has not been identified to date (Hayaishi et al, J Biol Chem 236:781-90 (1961)). Methylmalonate semialdehyde dehydrogenase (acetylating) enzymes (EC 1.2.1.27) are also suitable candidates, as several enzymes in this class accept malonate semialdehyde as a substrate including Msdh of Bacillus subtilis (Stines-Chaumeil et al, Biochem J 395:107-15 (2006)) and the methylmalonate semialdehyde dehydrogenase of R. norvegicus (Kedishvii et al, Methods Enzymol 324:207-18 (2000)).

Protein GenBank ID GI Number Organism ddcC ACV84070.1 258618587 Halomonas sp. HTNK1 ANI_1_1120014 XP_001389265.1 145229913 Aspergillus niger ALD6 XP_710976.1 68490403 Candida albicans YALI0C01859g XP_501343.1 50547747 Yarrowia lipolytica mmsA_1 YP_257876.1 70734236 Pseudomonas fluorescens mmsA_2 YP_257884.1 70734244 Pseudomonas fluorescens PA0130 NP_248820.1 15595328 Pseudomonas aeruginosa Mmsdh Q02253.1 400269 Rattus norvegicus msdh NP_391855.1 16081027 Bacillus subtilis IolA ABP57762.1 145309085 Lactobacillus casei

2.7.2.a Kinase

Pyruvate kinase (Step 10N), also known as phosphoenolpyruvate synthase (EC 2.7.9.2), converts pyruvate and ATP to PEP and AMP. This enzyme is encoded by the PYK1 (Burke et al., J. Biol. Chem. 258:2193-2201 (1983)) and PYK2 (Boles et al., J. Bacteriol. 179:2987-2993 (1997)) genes in S. cerevisiae. In E. coli, this activity is catalyzed by the gene products of pykF and pykA. Selected homologs of the S. cerevisiae enzymes are also shown in the table below.

Protein GenBank ID GI Number Organism PYK1 NP_009362 6319279 Saccharomyces cerevisiae PYK2 NP_014992 6324923 Saccharomyces cerevisiae pykF NP_416191.1 16129632 Escherichia coli PykA NP_416368.1 16129807 Escherichia coli KLLA0F23397g XP_456122.1 50312181 Kluyveromyces lactis CaO19.3575 XP_714934.1 68482353 Candida albicans CaO19.11059 XP_714997.1 68482226 Candida albicans YALI0F09185p XP_505195 210075987 Yarrowia hpolytica ANI_1_1126064 XP_001391973 145238652 Aspergillus niger

2.83.a CoA Transferase

Activation of malonate to malonyl-CoA is catalyzed by a CoA transferase in EC class 2.8.3.a. Malonyl-CoA:acetate CoA transferase (EC 2.8.3.3) enzymes have been characterized in Pseudomonas species including Pseudomonas fluorescens and Pseudomonas putida (Takamura et al, Biochem Int 3:483-91 (1981); Hayaishi et al, J Biol Chem 215:125-36 (1955)). Genes associated with these enzymes have not been identified to date. A mitochondrial CoA transferase found in Rattus norvegicus liver also catalyzes this reaction and is able to utilize a range of CoA donors and acceptors (Deana et al, Biochem Int 26:767-73 (1992)). Several CoA transferase enzymes described herein can also be applied to catalyze step K of FIG. 4. These enzymes include acetyl-CoA transferase, 3-HB CoA transferase, acetoacetyl-CoA transferase, SCOT and other CoA transferases.

3.13.a Phosphatase

Phosphoenolpyruvate phosphatase (EC 3.1.3.60, FIG. 4, Step N) catalyzes the hydrolysis of PEP to pyruvate and phosphate. Numerous phosphatase enzymes catalyze this activity, including alkaline phosphatase (EC 3.1.3.1), acid phosphatase (EC 3.1.3.2), phosphoglycerate phosphatase (EC 3.1.3.20) and PEP phosphatase (EC 3.1.3.60). PEP phosphatase enzymes have been characterized in plants such as Vignia radiate, Bruguiera sexangula and Brassica nigra. The phytase from Aspergillus fumigates, the acid phosphatase from Homo sapiens and the alkaline phosphatase of E. coli also catalyze the hydrolysis of PEP to pyruvate (Brugger et al, Appl Microbiol Biotech 63:383-9 (2004); Hayman et al, Biochem J 261:601-9 (1989); et al, The Enzymes 3^(rd) Ed. 4:373-415 (1971))) Similar enzymes have been characterized in Campylobacter jejuni (van Mourik et al., Microbiol. 154:584-92 (2008)), Saccharomyces cerevisiae (Oshima et al., Gene 179:171-7 (1996)) and Staphylococcus aureus (Shah and Blobel, J. Bacteriol. 94:780-1 (1967)). Enzyme engineering and/or removal of targeting sequences may be required for alkaline phosphatase enzymes to function in the cytoplasm.

Protein GenBank ID GI Number Organism phyA O00092.1 41017447 Aspergillus fumigatus Acp5 P13686.3 56757583 Homo sapiens phoA NP_414917.2 49176017 Escherichia coli phoX ZP_01072054.1 86153851 Campylobacter jejuni PHO8 AAA34871.1 172164 Saccharomyces cerevisiae SaurJH1_2706 YP_001317815.1 150395140 Staphylococcus aureus

4.1.1.a Decarboxylase

Several reactions in FIG. 4 are catalyzed by decarboxylase enzymes in EC class 4.1.1, including oxaloacetate decarboxylase (Step B), malonyl-CoA decarboxylase (step D) and pyruvate carboxylase or carboxykinase (step A).

Carboxylation of phosphoenolpyruvate to oxaloacetate is catalyzed by phosphoenolpyruvate carboxylase (EC 4.1.1.31). Exemplary PEP carboxylase enzymes are encoded by ppc in E. coli (Kai et al., Arch. Biochem. Biophys. 414:170-179 (2003), ppcA in Methylobacterium extorquens AM1 (Arps et al., J. Bacteriol. 175:3776-3783 (1993), and ppc in Corynebacterium glutamicum (Eikmanns et al., Mol. Gen. Genet. 218:330-339 (1989).

Protein GenBank ID GI Number Organism Ppc NP_418391 16131794 Escherichia coli ppcA AAB58883 28572162 Methylobacterium extorquens Ppc ABB53270 80973080 Corynebacterium glutamicum

An alternative enzyme for carboxylating phosphoenolpyruvate to oxaloacetate is PEP carboxykinase (EC 4.1.1.32, 4.1.1.49), which simultaneously forms an ATP or GTP. In most organisms PEP carboxykinase serves a gluconeogenic function and converts oxaloacetate to PEP at the expense of one ATP. S. cerevisiae is one such organism whose native PEP carboxykinase, PCK1, serves a gluconeogenic role (Valdes-Hevia et al., FEBS Lett. 258:313-316 (1989). E. coli is another such organism, as the role of PEP carboxykinase in producing oxaloacetate is believed to be minor when compared to PEP carboxylase (Kim et al., Appl. Environ. Microbiol. 70:1238-1241 (2004)). Nevertheless, activity of the native E. coli PEP carboxykinase from PEP towards oxaloacetate has been recently demonstrated in ppc mutants of E. coli K-12 (Kwon et al., J. Microbiol. Biotechnol. 16:1448-1452 (2006)). These strains exhibited no growth defects and had increased succinate production at high NaHCO₃ concentrations. Mutant strains of E. coli can adopt Pck as the dominant CO₂-fixing enzyme following adaptive evolution (Zhang et al. 2009). In some organisms, particularly rumen bacteria, PEP carboxykinase is quite efficient in producing oxaloacetate from PEP and generating ATP. Examples of PEP carboxykinase genes that have been cloned into E. coli include those from Mannheimia succiniciproducens (Lee et al., Biotechnol. Bioprocess Eng. 7:95-99 (2002)), Anaerobiospirillum succiniciproducens (Laivenieks et al., Appl. Environ. Microbiol. 63:2273-2280 (1997), and Actinobacillus succinogenes (Kim et al. supra). The PEP carboxykinase enzyme encoded by Haemophilus influenza is effective at forming oxaloacetate from PEP. Another suitable candidate is the PEPCK enzyme from Megathyrsus maximus, which has a low Km for CO₂, a substrate thought to be rate-limiting in the E. coli enzyme (Chen et al., Plant Physiol 128:160-164 (2002); Cotelesage et al., Int. J Biochem. Cell Biol. 39:1204-1210 (2007)). The kinetics of the GTP-dependent pepck gene product from Cupriavidus necator favor oxaloacetate formation (U.S. Pat. No. 8,048,624 and Lea et al, Amino Acids 20:225-41 (2001)).

Protein GenBank ID GI Number Organism PCK1 NP_013023 6322950 Saccharomyces cerevisiae pck NP_417862.1 16131280 Escherichia coli pckA YP_089485.1 52426348 Mannheimia succiniciproducens pckA O09460.1 3122621 Anaerobiospirillum succiniciproducens pckA Q6W6X5 75440571 Actinobacillus succinogenes pckA P43923.1 1172573 Haemophilus influenza AF532733.1:1 . . . 1929 AAQ10076.1 33329363 Megathyrsus maximus pepck YP_728135.1 113869646 Cupriavidus necator

Oxaloacetate decarboxylase catalyzes the decarboxylation of oxaloacetate to malonate semialdehyde. Enzymes catalyzing this reaction include kgd of Mycobacterium tuberculosis (GenBank ID: 050463.4, GI: 160395583) Enzymes evolved from kgd with improved activity and/or substrate specificity for oxaloacetate have also been described (U.S. Pat. No. 8,048,624). Additional enzymes useful for catalyzing this reaction include keto-acid decarboxylases shown in the table below.

EC number Name 4.1.1.1 Pyruvate decarboxylase 4.1.1.7 Benzoylformate decarboxylase 4.1.1.40 Hydroxypyruvate decarboxylase 4.1.1.43 Ketophenylpyruvate decarboxylase 4.1.1.71 Alpha-ketoglutarate decarboxylase 4.1.1.72 Branched chain keto-acid decarboxylase 4.1.1.74 Indolepyruvate decarboxylase 4.1.1.75 2-Ketoarginine decarboxylase 4.1.1.79 Sulfopyruvate decarboxylase 4.1.1.80 Hydroxyphenylpyruvate decarboxylase 4.1.1.82 Phosphonopyruvate decarboxylase

The decarboxylation of keto-acids is catalyzed by a variety of enzymes with varied substrate specificities, including pyruvate decarboxylase (EC 4.1.1.1), benzoylformate decarboxylase (EC 4.1.1.7), alpha-ketoglutarate decarboxylase and branched-chain alpha-ketoacid decarboxylase. Pyruvate decarboxylase (PDC), also termed keto-acid decarboxylase, is a key enzyme in alcoholic fermentation, catalyzing the decarboxylation of pyruvate to acetaldehyde. The PDC1 enzyme from Saccharomyces cerevisiae has a broad substrate range for aliphatic 2-keto acids including 2-ketobutyrate, 2-ketovalerate, 3-hydroxypyruvate and 2-phenylpyruvate (22). This enzyme has been extensively studied, engineered for altered activity, and functionally expressed in E. coli (Killenberg-Jabs et al., Eur. J. Biochem. 268:1698-1704 (2001); Li et al., Biochemistry. 38:10004-10012 (1999); ter Schure et al., Appl. Environ. Microbiol. 64:1303-1307 (1998)). The PDC from Zymomonas mobilus, encoded by pdc, also has a broad substrate range and has been a subject of directed engineering studies to alter the affinity for different substrates (Siegert et al., Protein Eng Des Sel 18:345-357 (2005)). The crystal structure of this enzyme is available (Killenberg-Jabs et al., Eur. J. Biochem. 268:1698-1704 (2001)). Other well-characterized PDC candidates include the enzymes from Acetobacter pasteurians (Chandra et al., 176:443-451 (2001)) and Kluyveromyces lactis (Krieger et al., 269:3256-3263 (2002)).

Protein GenBank ID GI Number Organism pdc P06672.1 118391 Zymomonas mobilis pdc1 P06169 30923172 Saccharomyces cerevisiae pdc Q8L388 20385191 Acetobacter pasteurians pdc1 Q12629 52788279 Kluyveromyces lactis

Like PDC, benzoylformate decarboxylase (EC 4.1.1.7) has a broad substrate range and has been the target of enzyme engineering studies. The enzyme from Pseudomonas putida has been extensively studied and crystal structures of this enzyme are available (Polovnikova et al., 42:1820-1830 (2003); Hasson et al., 37:9918-9930 (1998)). Site-directed mutagenesis of two residues in the active site of the Pseudomonas putida enzyme altered the affinity (Km) of naturally and non-naturally occurring substrates (Siegert et al., Protein Eng Des Sel 18:345-357 (2005)). The properties of this enzyme have been further modified by directed engineering (Lingen et al., Chembiochem. 4:721-726 (2003); Lingen et al., Protein Eng 15:585-593 (2002)). The enzyme from Pseudomonas aeruginosa, encoded by mdlC, has also been characterized experimentally (Barrowman et al., 34:57-60 (1986)). Additional gene candidates from Pseudomonas stutzeri, Pseudomonas fluorescens and other organisms can be inferred by sequence homology or identified using a growth selection system developed in Pseudomonas putida (Henning et al., Appl. Environ. Microbiol. 72:7510-7517 (2006)).

Protein GenBank ID GI Number Organism mdlC P20906.2 3915757 Pseudomonas putida mdlC Q9HUR2.1 81539678 Pseudomonas aeruginosa dpgB ABN80423.1 126202187 Pseudomonas stutzeri ilvB-1 YP_260581.1 70730840 Pseudomonas fluorescens

A third enzyme capable of decarboxylating 2-oxoacids is alpha-ketoglutarate decarboxylase (KGD, EC 4.1.1.71). The substrate range of this class of enzymes has not been studied to date. An exemplary KDC is encoded by kad in Mycobacterium tuberculosis (Tian et al., PNAS 102:10670-10675 (2005)). KDC enzyme activity has also been detected in several species of rhizobia including Bradyrhizobium japonicum and Mesorhizobium loti (Green et al., J Bacteriol 182:2838-2844 (2000)). Although the KDC-encoding gene(s) have not been isolated in these organisms, the genome sequences are available and several genes in each genome are annotated as putative KDCs. A KDC from Euglena gracilis has also been characterized but the gene associated with this activity has not been identified to date (Shigeoka et al., Arch. Biochem. Biophys. 288:22-28 (1991)). The first twenty amino acids starting from the N-terminus were sequenced MTYKAPVKDVKFLLDKVFKV (Shigeoka and Nakano, Arch. Biochem. Biophys. 288:22-28 (1991)). The gene could be identified by testing candidate genes containing this N-terminal sequence for KDC activity. A novel class of AKG decarboxylase enzymes has recently been identified in cyanobacteria such as Synechococcus sp. PCC 7002 and homologs (Zhang and Bryant, Science 334:1551-3 (2011)).

Protein GenBank ID GI Number Organism kgd O50463.4 160395583 Mycobacterium tuberculosis kgd NP_767092.1 27375563 Bradyrhizobium japonicum USDA110 kgd NP_105204.1 13473636 Mesorhizobium loti ilvB ACB00744.1 169887030 Synechococcus sp. PCC 7002

A fourth candidate enzyme for catalyzing this reaction is branched chain alpha-ketoacid decarboxylase (BCKA). This class of enzyme has been shown to act on a variety of compounds varying in chain length from 3 to 6 carbons (Mu et al., J Biol Chem. 263:18386-18396 (1988); Smit et al., Appl Environ Microbiol 71:303-311 (2005)). The enzyme in Lactococcus lactis has been characterized on a variety of branched and linear substrates including 2-oxobutanoate, 2-oxohexanoate, 2-oxopentanoate, 3-methyl-2-oxobutanoate, 4-methyl-2-oxobutanoate and isocaproate (Smit et al., Appl Environ Microbiol 71:303-311 (2005)). The enzyme has been structurally characterized (Berg et al., Science. 318:1782-1786 (2007)). Sequence alignments between the Lactococcus lactis enzyme and the pyruvate decarboxylase of Zymomonas mobilus indicate that the catalytic and substrate recognition residues are nearly identical (Siegert et al., Protein Eng Des Sel 18:345-357 (2005)), so this enzyme would be a promising candidate for directed engineering. Several ketoacid decarboxylases of Saccharomyces cerevisiae catalyze the decarboxylation of branched substrates, including ARO10, PDC6, PDC5, PDC1 and THI3 (Dickenson et al, J Biol Chem 275:10937-42 (2000)). Yet another BCKAD enzyme is encoded by rv0853c of Mycobacterium tuberculosis (Werther et al, J Biol Chem 283:5344-54 (2008)). This enzyme is subject to allosteric activation by alpha-ketoacid substrates. Decarboxylation of alpha-ketoglutarate by a BCKA was detected in Bacillus subtilis; however, this activity was low (5%) relative to activity on other branched-chain substrates (Mu and Kaneda, J Biol Chem. 263:18386-18396 (1988)) and the gene encoding this enzyme has not been identified to date. Additional BCKA gene candidates can be identified by homology to the Lactococcus lactis protein sequence. Many of the high-scoring BLASTp hits to this enzyme are annotated as indolepyruvate decarboxylases (EC 4.1.1.74). Indolepyruvate decarboxylase (IPDA) is an enzyme that catalyzes the decarboxylation of indolepyruvate to indoleacetaldehyde in plants and plant bacteria. Recombinant branched chain alpha-keto acid decarboxylase enzymes derived from the E1 subunits of the mitochondrial branched-chain keto acid dehydrogenase complex from Homo sapiens and Bos taurus have been cloned and functionally expressed in E. coli (Davie et al., J. Biol. Chem. 267:16601-16606 (1992); Wynn et al., J. Biol. Chem. 267:12400-12403 (1992); Wynn et al., J. Biol. Chem. 267:1881-1887 (1992)). In these studies, the authors found that co-expression of chaperonins GroEL and GroES enhanced the specific activity of the decarboxylase by 500-fold (Wynn et al., J. Biol. Chem. 267:12400-12403 (1992)). These enzymes are composed of two alpha and two beta subunits.

Protein GenBank ID GI Number Organism kdcA AAS49166.1 44921617 Lactococcus lactis PDC6 NP_010366.1 6320286 Saccharomyces cerevisiae PDC5 NP_013235.1 6323163 Saccharomyces cerevisiae PDC1 P06169 30923172 Saccharomyces cerevisiae ARO10 NP_010668.1 6320588 Saccharomyces cerevisiae THI3 NP_010203.1 6320123 Saccharomyces cerevisiae rv0853c O53865.1 81343167 Mycobacterium tuberculosis BCKDHB NP_898871.1 34101272 Homo sapiens BCKDHA NP_000700.1 11386135 Homo sapiens BCKDHB P21839 115502434 Bos taurus BCKDHA P11178 129030 Bos taurus

3-Phosphonopyruvate decarboxylase (EC 4.1.1.82) catalyzes the decarboxylation of 3-phosphonopyruvate to 2-phosphonoacetaldehyde. Exemplary phosphonopyruvate decarboxylase enzymes are encoded by dhpF of Streptomyces luridus, ppd of Streptomyces viridochromogenes, fom2 of Streptomyces wedmorensis and bcpC of Streptomyces hygroscopius (Circello et al, Chem Biol 17:402-11 (2010); Blodgett et al, FEMS Microbiol Lett 163:149-57 (2005); Hidaka et al, Mol Gen Genet 249:274-80 (1995); Nakashita et al, Biochim Biophys Acta 1490:159-62 (2000)). The Bacteroides fragilis enzyme, encoded by aepY, also decarboxylates pyruvate and sulfopyruvate (Zhang et al, J Biol Chem 278:41302-8 (2003)).

Protein GenBank ID GI Number Organism dhpF ACZ13457.1 268628095 Streptomyces luridus Ppd CAJ14045.1 68697716 Streptomyces viridochromogenes Fom2 BAA32496.1 1061008 Streptomyces wedmorensis aepY AAG26466.1 11023509 Bacteroides fragilis

Many oxaloacetate decarboxylase enzymes such as the eda gene product in E. coli (EC 4.1.1.3), act on the terminal acid of oxaloacetate to form pyruvate. Because decarboxylation at the 3-keto acid position competes with the malonate semialdehyde forming decarboxylation at the 2-keto-acid position, this enzyme activity can be knocked out in a host strain with a pathway proceeding through a malonate semilaldehyde intermediate.

Malonyl-CoA decarboxylase (EC 4.1.1.9) catalyzes the decarboxylation of malonyl-CoA to acetyl-CoA. Enzymes have been characterized in Rhizobium leguminosarum and Acinetobacter calcoaceticus (An et al, Eur J Biochem 257: 395-402 (1998); Koo et al, Eur J Biochem 266:683-90 (1999)) Similar enzymes have been characterized in Streptomyces erythreus (Hunaiti et al, Arch Biochem Biophys 229:426-39 (1984)). A recombinant human malonyl-CoA decarboxylase was overexpressed in E. coli (Zhou et al, Prot Expr Pur 34:261-9 (2004)). Methylmalonyl-CoA decarboxylase enzymes that decarboxylate malonyl-CoA are also suitable candidates. For example, the Veillonella parvula enzyme accepts malonyl-CoA as a substrate (Hilpert et al, Nature 296:584-5 (1982)). The E. coli enzyme is encoded by ygfG (Benning et al., Biochemistry. 39:4630-4639 (2000); Haller et al., Biochemistry. 39:4622-4629 (2000)). The stereo specificity of the E. coli enzyme was not reported, but the enzyme in Propionigenium modestum (Bott et al., Eur. J. Biochem. 250:590-599 (1997)) and Veillonella parvula (Huder et al., J. Biol. Chem. 268:24564-24571 (1993)) catalyzes the decarboxylation of the (S)-stereoisomer of methylmalonyl-CoA (Hoffmann et al., FEBS. Lett. 220:121-125 (1987)). The enzymes from P. modestum and V. parvula are comprised of multiple subunits that not only decarboxylate (S)-methylmalonyl-CoA, but also create a pump that transports sodium ions across the cell membrane as a means to generate energy.

Protein GenBank ID GI Number Organism YgfG NP_417394 90111512 Escherichia coli matA Q9ZIP6 75424899 Rhizobium leguminosarum mdcD AAB97628.1 2804622 Acinetobacter calcoaceticus mdcE AAF20287.1 6642782 Acinetobacter calcoaceticus mdcA AAB97627.1 2804621 Acinetobacter calcoaceticus mdcC AAB97630.1 2804624 Acinetobacter calcoaceticus mcd NP_036345.2 110349750 Homo sapiens mmdA CAA05137 2706398 Propionigenium modestum mmdD CAA05138 2706399 Propionigenium modestum mmdC CAA05139 2706400 Propionigenium modestum mmdB CAA05140 2706401 Propionigenium modestum mmdA CAA80872 415915 Veillonella parvula mmdC CAA80873 415916 Veillonella parvula mmdE CAA80874 415917 Veillonella parvula mmdD CAA80875 415918 Veillonella parvula mmdB CAA80876 415919 Veillonella parvula

6.2.1.a CoA Synthetase

Activation of malonate to malonyl-CoA is catalyzed by a CoA synthetase in EC class 6.2.1.a. CoA synthetase enzymes that catalyze this reaction have not been described in the literature to date. Several CoA synthetase enzymes described above can also be applied to catalyze step K of FIG. 4. These enzymes include acetyl-CoA synthetase and ADP forming CoA synthetases.

6.4.1.a Carboxylase

Pyruvate carboxylase (EC 6.4.1.1) converts pyruvate to oxaloacetate at the cost of one ATP (step H). Exemplary pyruvate carboxylase enzymes are encoded by PYC1 (Walker et al., Biochem. Biophys. Res. Commun. 176:1210-1217 (1991) and PYC2 (Walker et al., supra) in Saccharomyces cerevisiae, and pyc in Mycobacterium smegmatis (Mukhopadhyay and Purwantini, Biochim. Biophys. Acta 1475:191-206 (2000)).

Protein GenBank ID GI Number Organism PYC1 NP_011453 6321376 Saccharomyces cerevisiae PYC2 NP_009777 6319695 Saccharomyces cerevisiae Pyc YP_890857.1 118470447 Mycobacterium smegmatis

Example VII Pathways for Producing Cytosolic Acetyl-CoA from Mitochondrial Acetyl-CoA

A mechanism for transporting acetyl-CoA from the mitochondrion to the cytosol can facilitate deployment of a cytosolic fatty alcohol, fatty aldehyde or fatty acid production pathway that originates from acetyl-CoA. Exemplary mechanisms for exporting acetyl-CoA include those depicted in FIGS. 5 and 6, which can involve forming citrate from acetyl-CoA and oxaloacetate in the mitochondrion, exporting the citrate from the mitochondrion to the cytosol, and converting the citrate to oxaloacetate and either acetate or acetyl-CoA. In certain embodiments, provided herein are methods for engineering a eukaryotic organism to increase its availability of cytosolic acetyl-CoA by introducing enzymes capable of carrying out the transformations depicted in any one of FIGS. 5 and 6. Exemplary enzymes capable of carrying out the required transformations are also disclosed herein.

The production of cytosolic acetyl-CoA from mitochondrial acetyl-CoA can be accomplished by a number of pathways, for example, in three to five enzymatic steps. In one exemplary pathway, mitochondrial acetyl-CoA and oxaloacetate are combined into citrate by a citrate synthase and the citrate is exported out of the mitochondrion by a citrate or citrate/oxaloacetate transporter. Enzymatic conversion of the citrate in the cytosol results in cytosolic acetyl-CoA and oxaloacetate. The cytosolic oxaloacetate can then optionally be transported back into the mitochondrion by an oxaloacetate transporter and/or a citrate/oxaloacetate transporter. In another exemplary pathway, the cytosolic oxaloacetate is first enzymatically converted into malate in the cytosol and then optionally transferred into the mitochondrion by a malate transporter and/or a malate/citrate transporter. Mitochondrial malate can then be converted into oxaloacetate with a mitochondrial malate dehydrogenase.

In yet another exemplary pathway, mitochondrial acetyl-CoA can be converted to cytosolic acetyl-CoA via a citramalate intermediate. For example, mitochondrial acetyl-CoA and pyruvate are converted to citramalate by citramalate synthase. Citramalate can then be transported into the cytosol by a citramalate or dicarboxylic acid transporter. Cytosolic acetyl-CoA and pyruvate are then regenerated from citramalate, directly or indirectly, and the pyruvate can re-enter the mitochondria.

Along these lines, several exemplary acetyl-CoA pathways for the production of cytosolic acetyl-CoA from mitochondrial acetyl-CoA are shown in FIGS. 5 and 6. In one embodiment, mitochondrial oxaloacetate is combined with mitochondrial acetyl-CoA to form citrate by a citrate synthase. The citrate is transported outside of the mitochondrion by a citrate transporter, a citrate/oxaloacetate transporter or a citrate/malate transporter. Cytosolic citrate is converted into cytosolic acetyl-CoA and oxaloacetate by an ATP citrate lyase. In another pathway, cytosolic citrate is converted into acetate and oxaloacetate by a citrate lyase. Acetate can then be converted into cytosolic acetyl-CoA by an acetyl-CoA synthetase or transferase. Alternatively, acetate can be converted by an acetate kinase to acetyl phosphate, and the acetyl phosphate can be converted to cytosolic acetyl-CoA by a phosphotransacetylase. Exemplary enzyme candidates for acetyl-CoA pathway enzymes are described below.

The conversion of oxaloacetate and mitochondrial acetyl-CoA is catalyzed by a citrate synthase (FIGS. 5 and 6, step A). In certain embodiments, the citrate synthase is expressed in a mitochondrion of a non-naturally occurring eukaryotic organism provided herein.

Protein GenBank ID GI number Organism CIT1 NP_014398.1 6324328 Saccharomyces cerevisiae S288c CIT2 NP_009931.1 6319850 Saccharomyces cerevisiae S288c CIT3 NP_015325.1 6325257 Saccharomyces cerevisiae S288c YALI0E02684p XP_503469.1 50551989 Yarrowia lipolytica YALI0E00638p XP_503380.1 50551811 Yarrowia lipolytica ANI_1_876084 XP_001393983.1 145242820 Aspergillus niger CBS 513.88 ANI_1_1474074 XP_001393195.2 317030721 Aspergillus niger CBS 513.88 ANI_1_2950014 XP_001389414.2 317026339 Aspergillus niger CBS 513.88 ANI_1_1226134 XP_001396731.1 145250435 Aspergillus niger CBS 513.88 gltA NP_415248.1 16128695 Escherichia coli K-12 MG1655

Transport of citrate from the mitochondrion to the cytosol can be carried out by several transport proteins. Such proteins either export citrate directly (i.e., citrate transporter, FIGS. 5 and 6, step B) to the cytosol or export citrate to the cytosol while simultaneously transporting a molecule such as malate (i.e., citrate/malate transporter, FIG. 5, step C) or oxaloacetate (i.e., citrate/oxaloacetate transporter FIG. 6, step C) from the cytosol into the mitochondrion as shown in FIGS. 5 and 6. Exemplary transport enzymes that carry out these transformations are provided in the table below.

Protein GenBank ID GI number Organism CTP1 NP_009850.1 6319768 Saccharomyces cerevisiae S288c YALI0F26323p XP_505902.1 50556988 Yarrowia lipolytica ATEG_09970 EAU29419.1 114187719 Aspergillus terreus NIH2624 KLLA0E18723g XP_454797.1 50309571 Kluyveromyces lactis NRRL Y-1140 CTRG_02320 XP_002548023.1 255726194 Candida tropicalis MYA-3404 ANI_1_1474094 XP_001395080.1 145245625 Aspergillus niger CBS 513.88 YHM2 NP_013968.1 6323897 Saccharomyces cerevisiae S288c DTC CAC84549.1 19913113 Arabidopsis thaliana DTC1 CAC84545.1 19913105 Nicotiana tabacum DTC2 CAC84546.1 19913107 Nicotiana tabacum DTC3 CAC84547.1 19913109 Nicotiana tabacum DTC4 CAC84548.1 19913111 Nicotiana tabacum DTC AAR06239.1 37964368 Citrus junos

ATP citrate lyase (ACL, EC 2.3.3.8, FIGS. 5 and 6, step D), also called ATP citrate synthase, catalyzes the ATP-dependent cleavage of citrate to oxaloacetate and acetyl-CoA. In certain embodiments, ATP citrate lyase is expressed in the cytosol of a eukaryotic organism. ACL is an enzyme of the RTCA cycle that has been studied in green sulfur bacteria Chlorobium limicola and Chlorobium tepidum. The alpha(4)beta(4) heteromeric enzyme from Chlorobium limicola was cloned and characterized in E. coli (Kanao et al., Eur. J. Biochem. 269:3409-3416 (2002). The C. limicola enzyme, encoded by aclAB, is irreversible and activity of the enzyme is regulated by the ratio of ADP/ATP. The Chlorobium tepidum a recombinant ACL from Chlorobium tepidum was also expressed in E. coli and the holoenzyme was reconstituted in vitro, in a study elucidating the role of the alpha and beta subunits in the catalytic mechanism (Kim and Tabita, J. Bacteriol. 188:6544-6552 (2006). ACL enzymes have also been identified in Balnearium lithotrophicum, Sulfurihydrogenibium subterraneum and other members of the bacterial phylum Aquificae (Hugler et al., Environ. Microbiol. 9:81-92 (2007)). This activity has been reported in some fungi as well. Exemplary organisms include Sordaria macrospora (Nowrousian et al., Curr. Genet. 37:189-93 (2000)), Aspergillus nidulans and Yarrowia lipolytica (Hynes and Murray, Eukaryotic Cell, July: 1039-1048, (2010), and Aspergillus niger (Meijer et al. J. Ind. Microbiol. Biotechnol. 36:1275-1280 (2009). Other candidates can be found based on sequence homology. Information related to these enzymes is tabulated below.

Protein GenBank ID GI Number Organism aclA BAB21376.1 12407237 Chlorobium limicola aclB BAB21375.1 12407235 Chlorobium limicola aclA AAM72321.1 21647054 Chlorobium tepidum aclB AAM72322.1 21647055 Chlorobium tepidum aclB ABI50084.1 114055039 Sulfurihydrogenibium subterraneum aclA AAX76834.1 62199504 Sulfurimonas denitrificans aclB AAX76835.1 62199506 Sulfurimonas denitrificans acl1 XP_504787.1 50554757 Yarrowia lipolytica acl2 XP_503231.1 50551515 Yarrowia lipolytica SPBC1703.07 NP_596202.1 19112994 Schizosaccharomyces pombe SPAC22A12.16 NP_593246.1 19114158 Schizosaccharomyces pombe acl1 CAB76165.1 7160185 Sordaria macrospora acl2 CAB76164.1 7160184 Sordaria macrospora aclA CBF86850.1 259487849 Aspergillus nidulans aclB CBF86848 259487848 Aspergillus nidulans

In some organisms the conversion of citrate to oxaloacetate and acetyl-CoA proceeds through a citryl-CoA intermediate and is catalyzed by two separate enzymes, citryl-CoA synthetase (EC 6.2.1.18) and citryl-CoA lyase (EC 4.1.3.34) (Aoshima, M., Appl. Microbiol. Biotechnol. 75:249-255 (2007). Citryl-CoA synthetase catalyzes the activation of citrate to citryl-CoA. The Hydrogenobacter thermophilus enzyme is composed of large and small subunits encoded by ccsA and ccsB, respectively (Aoshima et al., Mol. Micrbiol. 52:751-761 (2004)). The citryl-CoA synthetase of Aquifex aeolicus is composed of alpha and beta subunits encoded by sucC1 and sucD1 (Hugler et al., Environ. Microbiol. 9:81-92 (2007)). Citryl-CoA lyase splits citryl-CoA into oxaloacetate and acetyl-CoA. This enzyme is a homotrimer encoded by cc/in Hydrogenobacter thermophilus (Aoshima et al., Mol. Microbiol. 52:763-770 (2004)) and aq_150 in Aquifex aeolicus (Hugler et al., supra (2007)). The genes for this mechanism of converting citrate to oxaloacetate and citryl-CoA have also been reported recently in Chlorobium tepidum (Eisen et al., PNAS 99(14): 9509-14 (2002)).

Protein GenBank ID GI Number Organism ccsA BAD17844.1 46849514 Hydrogenobacter thermophilus ccsB BAD17846.1 46849517 Hydrogenobacter thermophilus sucC1 AAC07285 2983723 Aquifex aeoticus sucD1 AAC07686 2984152 Aquifex aeoticus ccl BAD17841.1 46849510 Hydrogenobacter thermophilus aq_150 AAC06486 2982866 Aquifex aeolicus CT0380 NP_661284 21673219 Chlorobium tepidum CT0269 NP_661173.1 21673108 Chlorobium tepidum CT1834 AAM73055.1 21647851 Chlorobium tepidum

Citrate lyase (EC 4.1.3.6, FIGS. 5 and 6, step E) catalyzes a series of reactions resulting in the cleavage of citrate to acetate and oxaloacetate. In certain embodiments, citrate lyase is expressed in the cytosol of a eukaryotic organism. The enzyme is active under anaerobic conditions and is composed of three subunits: an acyl-carrier protein (ACP, gamma), an ACP transferase (alpha), and an acyl lyase (beta) Enzyme activation uses covalent binding and acetylation of an unusual prosthetic group, 2′-(5″-phosphoribosyl)-3-′-dephospho-CoA, which is similar in structure to acetyl-CoA. Acylation is catalyzed by CitC, a citrate lyase synthetase. Two additional proteins, CitG and CitX, are used to convert the apo enzyme into the active holo enzyme (Schneider et al., Biochemistry 39:9438-9450 (2000)). Wild type E. coli does not have citrate lyase activity; however, mutants deficient in molybdenum cofactor synthesis have an active citrate lyase (Clark, FEMS Microbiol. Lett. 55:245-249 (1990)). The E. coli enzyme is encoded by citEFD and the citrate lyase synthetase is encoded by citC (Nilekani and SivaRaman, Biochemistry 22:4657-4663 (1983)). The Leuconostoc mesenteroides citrate lyase has been cloned, characterized and expressed in E. coli (Bekal et al., J. Bacteriol. 180:647-654 (1998)). Citrate lyase enzymes have also been identified in enterobacteria that utilize citrate as a carbon and energy source, including Salmonella typhimurium and Klebsiella pneumoniae (Bott, Arch. Microbiol. 167: 78-88 (1997); Bott and Dimroth, Mol. Microbiol. 14:347-356 (1994)). The aforementioned proteins are tabulated below.

Protein GenBank ID GI Number Organism citF AAC73716.1 1786832 Escherichia coli cite AAC73717.2 87081764 Escherichia coli citD AAC73718.1 1786834 Escherichia coli citC AAC73719.2 87081765 Escherichia coli citG AAC73714.1 1786830 Escherichia coli citX AAC73715.1 1786831 Escherichia coli citF CAA71633.1 2842397 Leuconostoc mesenteroides citE CAA71632.1 2842396 Leuconostoc mesenteroides citD CAA71635.1 2842395 Leuconostoc mesenteroides citC CAA71636.1 3413797 Leuconostoc mesenteroides citG CAA71634.1 2842398 Leuconostoc mesenteroides citX CAA71634.1 2842398 Leuconostoc mesenteroides citF NP_459613.1 16763998 Salmonella typhimurium citE AAL19573.1 16419133 Salmonella typhimurium citD NP_459064.1 16763449 Salmonella typhimurium citC NP_459616.1 16764001 Salmonella typhimurium citG NP_459611.1 16763996 Salmonella typhimurium citX NP_459612.1 16763997 Salmonella typhimurium citF CAA56217.1 565619 Klebsiella pneumoniae citE CAA56216.1 565618 Klebsiella pneumoniae citD CAA56215.1 565617 Klebsiella pneumoniae citC BAH66541.1 238774045 Klebsiella pneumoniae citG CAA56218.1 565620 Klebsiella pneumoniae citX AAL60463.1 18140907 Klebsiella pneumoniae

The acylation of acetate to acetyl-CoA is catalyzed by enzymes with acetyl-CoA synthetase activity (FIGS. 5 and 6, step F). In certain embodiments, acetyl-CoA synthetase is expressed in the cytosol of a eukaryotic organism. Two enzymes that catalyze this reaction are AMP-forming acetyl-CoA synthetase (EC 6.2.1.1) and ADP-forming acetyl-CoA synthetase (EC 6.2.1.13). AMP-forming acetyl-CoA synthetase (ACS) is the predominant enzyme for activation of acetate to acetyl-CoA. Exemplary ACS enzymes are found in E. coli (Brown et al., J. Gen. Microbiol. 102:327-336 (1977)), Ralstonia eutropha (Priefert and Steinbuchel, J. Bacteriol. 174:6590-6599 (1992)), Methanothermobacter thermautotrophicus (Ingram-Smith and Smith, Archaea 2:95-107 (2007)), Salmonella enterica (Gulick et al., Biochemistry 42:2866-2873 (2003)) and Saccharomyces cerevisiae (Jogl and Tong, Biochemistry 43:1425-1431 (2004)).

Protein GenBank ID GI Number Organism acs AAC77039.1 1790505 Escherichia coli acoE AAA21945.1 141890 Ralstonia eutropha acs1 ABC87079.1 86169671 Methanothemobacter thermautotrophicus acs1 AAL23099.1 16422835 Salmonella enterica ACS1 Q01574.2 257050994 Saccharomyces cerevisiae

ADP-forming acetyl-CoA synthetase (ACD, EC 6.2.1.13) is another candidate enzyme that couples the conversion of acyl-CoA esters to their corresponding acids with the concurrent synthesis of ATP. Several enzymes with broad substrate specificities have been described in the literature. ACD I from Archaeoglobus fulgidus, encoded by AF1211, was shown to operate on a variety of linear and branched-chain substrates including acetyl-CoA, propionyl-CoA, butyryl-CoA, acetate, propionate, butyrate, isobutyryate, isovalerate, succinate, fumarate, phenylacetate, indoleacetate (Musfeldt et al., J. Bacteriol. 184:636-644 (2002)). The enzyme from Haloarcula marismortui (annotated as a succinyl-CoA synthetase) accepts propionate, butyrate, and branched-chain acids (isovalerate and isobutyrate) as substrates, and was shown to operate in the forward and reverse directions (Brasen et al., Arch. Microbiol. 182:277-287 (2004)). The ACD encoded by PAE3250 from hyperthermophilic crenarchaeon Pyrobaculum aerophilum showed the broadest substrate range of all characterized ACDs, reacting with acetyl-CoA, isobutyryl-CoA (preferred substrate) and phenylacetyl-CoA (Brasen et al., supra (2004)). The enzymes from A. fulgidus, H. marismortui and P. aerophilum have all been cloned, functionally expressed, and characterized in E. coli (Musfeldt et al., supra; Brasen et al., supra (2004)). Additional candidates include the succinyl-CoA synthetase encoded by sucCD in E. coli (Buck et. al., Biochemistry 24:6245-6252 (1985)) and the acyl-CoA ligase from Pseudomonas putida (Fernandez-Valverde et al., Appl. Environ. Microbiol. 59:1149-1154 (1993)). Information related to these proteins and genes is shown below.

Protein GenBank ID GI number Organism AF1211 NP_070039.1 11498810 Archaeoglobus fulgidus DSM 4304 AF1983 NP_070807.1 11499565 Archaeoglobus fulgidus DSM 4304 scs YP_135572.1 55377722 Haloarcula marismortui ATCC 43049 PAE3250 NP_560604.1 18313937 Pyrobaculum aerophilum str. IM2 sucC NP_415256.1 16128703 Escherichia coli sucD AAC73823.1 1786949 Escherichia coli paaF AAC24333.2 22711873 Pseudomonas putida

An alternative method for adding the CoA moiety to acetate is to apply a pair of enzymes such as a phosphate-transferring acyltransferase and an acetate kinase (FIGS. 5 and 6, Step F). This activity enables the net formation of acetyl-CoA with the simultaneous consumption of ATP. In certain embodiments, phosphotransacetylase is expressed in the cytosol of a eukaryotic organism. An exemplary phosphate-transferring acyltransferase is phosphotransacetylase, encoded by pta. The pta gene from E. coli encodes an enzyme that can convert acetyl-CoA into acetyl-phosphate, and vice versa (Suzuki, T. Biochim. Biophys. Acta 191:559-569 (1969)). This enzyme can also utilize propionyl-CoA instead of acetyl-CoA forming propionate in the process (Hesslinger et al. Mol. Microbiol 27:477-492 (1998)). Homologs exist in several other organisms including Salmonella enterica and Chlamydomonas reinhardtii.

Protein GenBank ID GI number Organism Pta NP_416800.1 16130232 Escherichia coli Pta NP_461280.1 16765665 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 PAT2 XP_001694504.1 159472743 Chlamydomonas reinhardtii PAT1 XP_001691787.1 159467202 Chlamydomonas reinhardtii

An exemplary acetate kinase is the E. coli acetate kinase, encoded by ackA (Skarstedt and Silverstein J. Biol. Chem. 251:6775-6783 (1976)). Homologs exist in several other organisms including Salmonella enterica and Chlamydomonas reinhardtii. Information related to these proteins and genes is shown below:

Protein GenBank ID GI number Organism AckA NP_416799.1 16130231 Escherichia coli AckA NP_461279.1 16765664 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 ACK1 XP_001694505.1 159472745 Chlamydomonas reinhardtii ACK2 XP_001691682.1 159466992 Chlamydomonas reinhardtii

In some embodiments, cytosolic oxaloacetate is transported back into a mitochondrion by an oxaloacetate transporter. Oxaloacetate transported back into a mitochondrion can then be used in the acetyl-CoA pathways described herein. Transport of oxaloacetate from the cytosol to the mitochondrion can be carried out by several transport proteins. Such proteins either import oxaloacetate directly (i.e., oxaloacetate transporter) to the mitochondrion or import oxaloacetate to the cytosol while simultaneously transporting a molecule such as citrate (i.e., citrate/oxaloacetate transporter) from the mitochondrion into the cytosol as shown in FIG. 6. Exemplary transport enzymes that carry out these transformations are provided in the table below.

Protein GenBank ID GI number Organism OAC1 NP_012802.1 6322729 Saccharomyces cerevisiae S288c KLLA0B12826g XP_452102.1 50304305 Kluyveromyces lactis NRRL Y-1140 YALI0E04048g XP_503525.1 50552101 Yarrowia lipolytica CTRG_02239 XP_002547942.1 255726032 Candida tropicalis MYA-3404 DIC1 NP_013452.1 6323381 Saccharomyces cerevisiae S288c YALI0B03344g XP_500457.1 50545838 Yarrowia lipolytica CTRG_02122 XP_002547815.1 255725772 Candida tropicalis MYA-3404 PAS_chr4_0877 XP_002494326.1 254574434 Pichia pastoris GS115 DTC CAC84549.1 19913113 Arabidopsis thaliana DTC1 CAC84545.1 19913105 Nicotiana tabacum DTC2 CAC84546.1 19913107 Nicotiana tabacum DTC3 CAC84547.1 19913109 Nicotiana tabacum DTC4 CAC84548.1 19913111 Nicotiana tabacum DTC AAR06239.1 37964368 Citrus junos

In some embodiments, cytosolic oxaloacetate is first converted to malate by a cytosolic malate dehydrogenase (FIG. 5, step H). Cytosolic malate is transported into a mitochondrion by a malate transporter or a citrate/malate transporter (FIG. 5, step I). Mitochondrial malate is then converted to oxaloacetate by a mitochondrial malate dehydrogenase (FIG. 5, step J). Mitochondrial oxaloacetate can then be used in the acetyl-CoA pathways described herein. Exemplary examples of each of these enzymes are provided below.

Oxaloacetate is converted into malate by malate dehydrogenase (EC 1.1.1.37, FIG. 5, step H). When malate is the dicarboxylate transported from the cytosol to mitochondrion, expression of both a cytosolic and mitochondrial version of malate dehydrogenase, e.g., as shown in FIG. 4, can be used. S. cerevisiae possesses three copies of malate dehydrogenase, MDH1 (McAlister-Henn and Thompson, J. Bacteriol. 169:5157-5166 (1987), MDH2 (Minard and McAlister-Henn, Mol. Cell. Biol. 11:370-380 (1991); Gibson and McAlister-Henn, J. Biol. Chem. 278:25628-25636 (2003)), and MDH3 (Steffan and McAlister-Henn, J. Biol. Chem. 267:24708-24715 (1992)), which localize to the mitochondrion, cytosol, and peroxisome, respectively. Close homologs to the cytosolic malate dehydrogenase, MDH2, from S. cerevisiae are found in several organisms including Kluyveromyces lactis and Candida tropicalis. E. coli is also known to have an active malate dehydrogenase encoded by mdh. In some embodiments, the exogenous malate dehydrogenase genes are Rhizopus delemar malate dehydrogenase genes encoding the amino acid sequence disclosed in WO2013112939 as SEQ ID NO:167 or its variants.

Protein GenBank ID GI Number Organism MDH1 NP_012838 6322765 Saccharomyces cerevisiae MDH2 NP_014515 116006499 Saccharomyces cerevisiae MDH3 NP_010205 6320125 Saccharomyces cerevisiae Mdh NP_417703.1 16131126 Escherichia coli KLLA0E07525p XP_454288.1 50308571 Kluyveromyces lactis NRRL Y-1140 YALI0D16753g XP_502909.1 50550873 Yarrowia lipolytica CTRG_01021 XP_002546239.1 255722609 Candida tropicalis MYA-3404

Transport of malate from the cytosol to the mitochondrion can be carried out by several transport proteins. Such proteins either import malate directly (i.e., malate transporter) to the mitochondrion or import malate to the cytosol while simultaneously transporting a molecule such as citrate (i.e., citrate/malate transporter) from the mitochondrion into the cytosol as shown in FIG. 5. Exemplary transport enzymes that carry out these transformations are provided in the table below.

Protein GenBank ID GI number Organism OAC1 NP_012802.1 6322729 Saccharomyces cerevisiae S288c KLLA0B12826g XP_452102.1 50304305 Kluyveromyces lactis NRRL Y-1140 YALI0E04048g XP_503525.1 50552101 Yarrowia lipolytica CTRG_02239 XP_002547942.1 255726032 Candida tropicalis MYA-3404 DIC1 NP_013452.1 6323381 Saccharomyces cerevisiae S288c YALI0B03344g XP_500457.1 50545838 Yarrowia lipolytica CTRG_02122 XP_002547815.1 255725772 Candida tropicalis MYA-3404 PAS_chr4_0877 XP_002494326.1 254574434 Pichia pastoris GS115 DTC CAC84549.1 19913113 Arabidopsis thaliana DTC1 CAC84545.1 19913105 Nicotiana tabacum DTC2 CAC84546.1 19913107 Nicotiana tabacum DTC3 CAC84547.1 19913109 Nicotiana tabacum DTC4 CAC84548.1 19913111 Nicotiana tabacum DTC AAR06239.1 37964368 Citrus junos

Malate can be converted into oxaloacetate by malate dehydrogenase (EC 1.1.1.37, FIG. 5, step J). When malate is the dicarboxylate transported from the cytosol to mitochondrion, in certain embodiments, both a cytosolic and mitochondrial version of malate dehydrogenase is expressed, as shown in FIGS. 4 and 5. S. cerevisiae possesses three copies of malate dehydrogenase, MDH1 (McAlister-Henn and Thompson, J. Bacteriol. 169:5157-5166 (1987), MDH2 (Minard and McAlister-Henn, Mol. Cell. Biol. 11:370-380 (1991); Gibson and McAlister-Henn, J. Biol. Chem. 278:25628-25636 (2003)), and MDH3 (Steffan and McAlister-Henn, J. Biol. Chem. 267:24708-24715 (1992)), which localize to the mitochondrion, cytosol, and peroxisome, respectively. Close homologs to the mitochondrial malate dehydrogenase, MDH1, from S. cerevisiae are found in several organisms including Kluyveromyces lactis, Yarrowia lipolytica, Candida tropicalis. E. coli is also known to have an active malate dehydrogenase encoded by mdh.

Protein GenBank ID GI Number Organism MDH1 NP_012838 6322765 Saccharomyces cerevisiae MDH2 NP_014515 116006499 Saccharomyces cerevisiae MDH3 NP_010205 6320125 Saccharomyces cerevisiae Mdh NP_417703.1 16131126 Escherichia coli KLLA0F25960g XP_456236.1 50312405 Kluyveromyces lactis NRRL Y-1140 YALI0D16753g XP_502909.1 50550873 Yarrowia lipolytica CTRG_00226 XP_002545445.1 255721021 Candida tropicalis MYA-3404

Example VIII Utilization of Pathway Enzymes with a Preference for NADH

The production of acetyl-CoA from glucose can generate at most four reducing equivalents in the form of NADH. A straightforward and energy efficient mode of maximizing the yield of reducing equivalents is to employ the Embden-Meyerhof-Parnas glycolysis pathway (EMP pathway). In many carbohydrate utilizing organisms, one NADH molecule is generated per oxidation of each glyceraldehyde-3-phosphate molecule by means of glyceraldehyde-3-phosphate dehydrogenase. Given that two molecules of glyceraldehyde-3-phosphate are generated per molecule of glucose metabolized via the EMP pathway, two NADH molecules can be obtained from the conversion of glucose to pyruvate.

Two additional molecules of NADH can be generated from conversion of pyruvate to acetyl-CoA given that two molecules of pyruvate are generated per molecule of glucose metabolized via the EMP pathway. This could be done by employing any of the following enzymes or enzyme sets to convert pyruvate to acetyl-CoA:

-   I. NAD-dependant pyruvate dehydrogenase; -   II. Pyruvate formate lyase and NAD-dependant formate dehydrogenase; -   III. Pyruvate:ferredoxin oxidoreductase and NADH:ferredoxin     oxidoreductase; -   IV. Pyruvate decarboxylase and an NAD-dependant acylating     acetylaldehyde dehydrogenase; -   V. Pyruvate decarboxylase, NAD-dependant acylating acetaldehyde     dehydrogenase, acetate kinase, and phosphotransacetylase; and -   VI. Pyruvate decarboxylase, NAD-dependant acylating acetaldehyde     dehydrogenase, and acetyl-CoA synthetase.

Overall, four molecules of NADH can be attained per glucose molecule metabolized. In one aspect, the fatty alcohol pathway requires three reduction steps from acetyl-CoA. Therefore, it can be possible that each of these three reduction steps will utilize NADPH or NADH as the reducing agents, in turn converting these molecules to NADP or NAD, respectively. Therefore, in some aspects, it can be desireable that all reduction steps are NADH-dependant in order to maximize the yield of fatty alcohols, fatty aldehydes or fatty acids. High yields of fatty alcohols, fatty aldehydes and fatty acids can thus be accomplished by:

-   I. Identifying and implementing endogenous or exogenous fatty     alcohol, fatty aldehyde or fatty acid pathway enzymes with a     stronger preference for NADH than other reducing equivalents such as     NADPH, -   II. Attenuating one or more endogenous fatty alcohol, fatty aldehyde     or fatty acid pathway enzymes that contribute NADPH-dependant     reduction activity, -   III. Altering the cofactor specificity of endogenous or exogenous     fatty alcohol, fatty aldehyde or fatty acid pathway enzymes so that     they have a stronger preference for NADH than their natural     versions, or -   IV. Altering the cofactor specificity of endogenous or exogenous     fatty alcohol, fatty aldehyde or fatty acid pathway enzymes so that     they have a weaker preference for NADPH than their natural versions.

Exemplary NADH-dependent enzymes that participate in the elongation cycle are shown in the table below.

Enzyme Substrate Gene Organism Multifunctional 3-ketoacyl-CoA fadB Escherichia coli ketoacyl-CoA Fox2 Candida tropicalis reductase/ FOX2 Saccharomyces epimerase/ cerevisiae dehydratase 3-Ketoacyl-CoA 3-ketoacyl-CoA paaH1 Ralstonia eutropha reductase 3HCDH Euglena gracilis Enoyl-CoA enoyl-CoA TDE0597 Treponema denticola reductase TER Euglena gracilis ECR1 Euglena gracilis ECR2 Euglena gracilis ECR3 Euglena gracilis acad1 Ascaris suum acad Ascaris suum acad Mycobacterium smegmatis

The individual enzyme or protein activities from the endogenous or exogenous DNA sequences can be assayed using methods well known in the art. For example, the genes can be expressed in E. coli and the activity of their encoded proteins can be measured using cell extracts. Alternatively, the enzymes can be purified using standard procedures well known in the art and assayed for activity. Spectrophotometric based assays are particularly effective.

Several examples and methods of altering the cofactor specificity of enzymes are known in the art. For example, Khoury et al. (Protein Sci. 2009 October; 18(10): 2125-2138) created several xylose reductase enzymes with an increased affinity for NADH and decreased affinity for NADPH. Ehsani et al (Biotechnology and Bioengineering, Volume 104, Issue 2, pages 381-389, 1 Oct. 2009) drastically decreased activity of 2,3-butanediol dehydrogenase on NADH while increasing activity on NADPH. Machielsen et al (Engineering in Life Sciences, Volume 9, Issue 1, pages 38-44, February 2009) dramatically increased activity of alcohol dehydrogenase on NADH. Khoury et al (Protein Sci. 2009 October; 18(10): 2125-2138) list in Table I several previous examples of successfully changing the cofactor preference of over 25 other enzymes. Additional descriptions can be found in Lutz et al, Protein Engineering Handbook, Volume 1 and Volume 2, 2009, Wiley-VCH Verlag GmbH & Co. KGaA, in particular, Chapter 31: Altering Enzyme Substrate and Cofactor Specificity via Protein Engineering.

Example IX Determining Cofactor Preference of Pathway Enzymes

This example describes an experimental method for determining the cofactor preference of an enzyme.

Cofactor preference of enzymes for each of the pathway steps can be determined by cloning the individual genes on a plasmid behind a constitutive or inducible promoter and transforming into a host organism such as Escherichia coli. For example, genes encoding enzymes that catalyze pathway steps from: 1) acetoacetyl-CoA to 3-hydroxybutyryl-CoA, 2) 3-hydroxybutyryl-CoA to 3-hydroxybutyraldehyde, 3) 3-hydroxybutyraldehyde to 1,3-butanediol (wherein R₁ is C₁; R₃ is OH) can be assembled onto the pZ-based expression vectors as described below.

Replacement of the Stuffer Fragment in the pZ-Based Expression Vectors.

Vector backbones were obtained from Dr. Rolf Lutz of Expressys (http://www.expressys.de/). The vectors and strains are based on the pZ Expression System developed by Lutz and Bujard (Nucleic Acids Res 25, 1203-1210 (1997)). The pZE13luc, pZA33luc, pZS*13luc and pZE22luc contain the luciferase gene as a stuffer fragment. To replace the luciferase stuffer fragment with a lacZ-alpha fragment flanked by appropriate restriction enzyme sites, the luciferase stuffer fragment is removed from each vector by digestion with EcoRI and XbaI. The lacZ-alpha fragment is PCR amplified from pUC19 with the following primers:

lacZalpha-RI (SEQ ID NO: 1) 5′GACGAATTCGCTAGCAAGAGGAGAAGTCGACATGTCCAATTCACTGG CCGTCGTTTTAC3′ lacZalpha 3′BB (SEQ ID NO: 2) 5′-GACCCTAGGAAGCTTTCTAGAGTCGACCTATGCGGCATCAGAGCAG A-3′

This generates a fragment with a 5′ end of EcoRI site, NheI site, a Ribosomal Binding Site, a SalI site and the start codon. On the 3′ end of the fragment are the stop codon, XbaI, HindIII, and AvrII sites. The PCR product is digested with EcoRI and AvrII and ligated into the base vectors digested with EcoRI and XbaI (XbaI and AvrII have compatible ends and generate a non-site). Because NheI and XbaI restriction enzyme sites generate compatible ends that can be ligated together (but generate a site after ligation that is not digested by either enzyme), the genes cloned into the vectors can be “Biobricked” together (http://openwetware.org/wild/Synthetic_Biology:BioBricks). Briefly, this method enables joining an unlimited number of genes into the vector using the same 2 restriction sites (as long as the sites do not appear internal to the genes), because the sites between the genes are destroyed after each addition. These vectors can be subsequently modified using the Phusion® Site-Directed Mutagenesis Kit (NEB, Ipswich, Mass., USA) to insert the spacer sequence AATTAA between the EcoRI and NheI sites. This eliminates a putative stem loop structure in the RNA that bound the RBS and start codon.

All vectors have the pZ designation followed by letters and numbers indicating the origin of replication, antibiotic resistance marker and promoter/regulatory unit. The origin of replication is the second letter and is denoted by E for ColE1, A for p15A and S for pSC101 (as well as a lower copy number version of pSC101 designated S*)—based origins. The first number represents the antibiotic resistance marker (1 for Ampicillin, 2 for Kanamycin, 3 for Chloramphenicol). The final number defines the promoter that regulated the gene of interest (1 for PLtetO-1, 2 for PLlacO-1 and 3 for PAllacO-1). For the work discussed here we employed three base vectors, pZS*13S, pZA33S and pZE13S, modified for the biobricks insertions as discussed above.

Plasmids containing genes encoding pathway enzymes can then transformed into host strains containing lacIQ, which allow inducible expression by addition of isopropyl β-D-1-thiogalactopyranoside (IPTG). Activities of the heterologous enzymes are tested in in vitro assays, using strain E. coli MG1655 lacIQ as the host for the plasmid constructs containing the pathway genes. Cells can be grown aerobically in LB media (Difco) containing the appropriate antibiotics for each construct, and induced by addition of IPTG at 1 mM when the optical density (0D600) reached approximately 0.5. Cells can be harvested after 6 hours, and enzyme assays conducted as discussed below.

In Vitro Enzyme Assays.

To obtain crude extracts for activity assays, cells can be harvested by centrifugation at 4,500 rpm (Beckman-Coulter, Allegera X-15R) for 10 min. The pellets are resuspended in 0.3 mL BugBuster (Novagen) reagent with benzonase and lysozyme, and lysis proceeds for about 15 minutes at room temperature with gentle shaking. Cell-free lysate is obtained by centrifugation at 14,000 rpm (Eppendorf centrifuge 5402) for 30 min at 4° C. Cell protein in the sample is determined using the method of Bradford et al., Anal. Biochem. 72:248-254 (1976), and specific enzyme assays conducted as described below. Activities are reported in Units/mg protein, where a unit of activity is defined as the amount of enzyme required to convert 1 micromol of substrate in 1 minute at room temperature.

Pathway steps can be assayed in the reductive direction using a procedure adapted from several literature sources (Durre et al., FEMS Microbiol. Rev. 17:251-262 (1995); Palosaari and Rogers, Bacteriol. 170:2971-2976 (1988) and Welch et al., Arch. Biochem. Biophys. 273:309-318 (1989). The oxidation of NADH or NADPH can be followed by reading absorbance at 340 nM every four seconds for a total of 240 seconds at room temperature. The reductive assays can be performed in 100 mM MOPS (adjusted to pH 7.5 with KOH), 0.4 mM NADH or 0.4 mM NADPH, and from 1 to 50 μmol of cell extract. For carboxylic acid reductase-like enzymes, ATP can also be added at saturating concentrations. The reaction can be started by adding the following reagents: 100 μmol of 100 mM acetoacetyl-CoA, 3-hydroxybutyryl-CoA, 3-hydroxybutyrate, or 3-hydroxybutyraldehyde. The spectrophotometer is quickly blanked and then the kinetic read is started. The resulting slope of the reduction in absorbance at 340 nM per minute, along with the molar extinction coefficient of NAD(P)H at 340 nM (6000) and the protein concentration of the extract, can be used to determine the specific activity.

Example X Methods for Increasing NADPH Availability

In some aspects of the invention, it can be advantageous to employ pathway enzymes that have activity using NADPH as the reducing agent. For example, NADPH-dependant pathway enzymes can be highly specific for MI-FAE cycle, MD-FAE cycle and/or termination pathway intermediates or can possess favorable kinetic properties using NADPH as a substrate. If one or more pathway steps is NADPH dependant, several alternative approaches to increase NADPH availability can be employed. These include:

-   -   1) Increasing flux relative to wild-type through the oxidative         branch of the pentose phosphate pathway comprising         glucose-6-phosphate dehydrogenase, 6-phosphogluconolactonase,         and 6-phosphogluconate dehydrogenase (decarboxylating). This         will generate 2 NADPH molecules per glucose-6-phosphate         metabolized. However, the decarboxylation step will reduce the         maximum theoretical yield of 1,3-butanediol.     -   2) Increasing flux relative to wild-type through the Entner         Doudoroff pathway comprising glucose-6-phosphate dehydrogenase,         6-phosphogluconolactonase, phosphogluconate dehydratase, and         2-keto-3-deoxygluconate 6-phosphate aldolase.     -   3) Introducing a soluble transhydrogenase to convert NADH to         NADPH.     -   4) Introducing a membrane-bound transhydrogenase to convert NADH         to NADPH.     -   5) Employing an NADP-dependant glyceraldehyde-3-phosphate         dehydrogenase.     -   6) Employing any of the following enzymes or enzyme sets to         convert pyruvate to acetyl-CoA         -   a) NADP-dependant pyruvate dehydrogenase;         -   b) Pyruvate formate lyase and NADP-dependant formate             dehydrogenase;         -   c) Pyruvate:ferredoxin oxidoreductase and NADPH:ferredoxin             oxidoreductase;         -   d) Pyruvate decarboxylase and an NADP-dependant acylating             acetylaldehyde dehydrogenase;         -   e) Pyruvate decarboxylase, NADP-dependant acetaldehyde             dehydrogenase, acetate kinase, and phosphotransacetylase;             and         -   f) Pyruvate decarboxylase, NADP-dependant acetaldehyde             dehydrogenase, and acetyl-CoA synthetase; and optionally             attenuating NAD-dependant versions of these enzymes.     -   7) Altering the cofactor specificity of a native         glyceraldehyde-3-phosphate dehydrogenase, pyruvate         dehydrogenase, formate dehydrogenase, or acylating         acetylaldehyde dehydrogenase to have a stronger preference for         NADPH than their natural versions.     -   8) Altering the cofactor specificity of a native         glyceraldehyde-3-phosphate dehydrogenase, pyruvate         dehydrogenase, formate dehydrogenase, or acylating         acetylaldehyde dehydrogenase to have a weaker preference for         NADH than their natural versions.

The individual enzyme or protein activities from the endogenous or exogenous DNA sequences can be assayed using methods well known in the art. For example, the genes can be expressed in E. coli and the activity of their encoded proteins can be measured using cell extracts as described in the previous example. Alternatively, the enzymes can be purified using standard procedures well known in the art and assayed for activity. Spectrophotometric based assays are particularly effective.

Several examples and methods of altering the cofactor specificity of enzymes are known in the art. For example, Khoury et al (Protein Sci. 2009 October; 18(10): 2125-2138) created several xylose reductase enzymes with an increased affinity for NADH and decreased affinity for NADPH. Ehsani et al (Biotechnology and Bioengineering, Volume 104, Issue 2, pages 381-389, 1 Oct. 2009) drastically decreased activity of 2,3-butanediol dehydrogenase on NADH while increasing activity on NADPH. Machielsen et al (Engineering in Life Sciences, Volume 9, Issue 1, pages 38-44, February 2009) dramatically increased activity of alcohol dehydrogenase on NADH. Khoury et al (Protein Sci. 2009 October; 18(10): 2125-2138) list in Table I several previous examples of successfully changing the cofactor preference of over 25 other enzymes. Additional descriptions can be found in Lutz et al, Protein Engineering Handbook, Volume 1 and Volume 2, 2009, Wiley-VCH Verlag GmbH & Co. KGaA, in particular, Chapter 31: Altering Enzyme Substrate and Cofactor Specificity via Protein Engineering.

Enzyme candidates for these steps are provided below.

Glucose-6-Phosphate Dehydrogenase

Protein GenBank ID GI Number Organism ZWF1 NP_014158.1 6324088 Saccharomyces cerevisiae S288c ZWF1 XP_504275.1 50553728 Yarrowia lipolytica Zwf XP_002548953.1 255728055 Candida tropicalis MYA-3404 Zwf XP_001400342.1 145233939 Aspergillus niger CBS 513.88 KLLA0D19855g XP_453944.1 50307901 Kluyveromyces lactis NRRL Y-1140

6-Phosphogluconolactonase

Protein GenBank ID GI Number Organism SOL3 NP_012033.2 82795254 Saccharomyces cerevisiae S288c SOL4 NP_011764.1 6321687 Saccharomyces cerevisiae S288c YALI0E11671g XP_503830.1 50552840 Yarrowia lipolytica YALI0C19085g XP_501998.1 50549055 Yarrowia lipolytica ANI_1_656014 XP_001388941.1 145229265 Aspergillus niger CBS 513.88 CTRG_00665 XP_002545884.1 255721899 Candida tropicalis MYA-3404 CTRG_02095 XP_002547788.1 255725718 Candida tropicalis MYA-3404 KLLA0A05390g XP_451238.1 50302605 Kluyveromyces lactis NRRL Y-1140 KLLA0C08415g XP_452574.1 50305231 Kluyveromyces lactis NRRL Y-1140

6-Phosphogluconate dehydrogenase (decarboxylating)

Protein GenBank ID GI Number Organism GND1 NP_012053.1 6321977 Saccharomyces cerevisiae S288c GND2 NP_011772.1 6321695 Saccharomyces cerevisiae S288c ANI_1_282094 XP_001394208.2 317032184 Aspergillus niger CBS 513.88 ANI_1_2126094 XP_001394596.2 317032939 Aspergillus niger CBS 513.88 YALI0B15598g XP_500938.1 50546937 Yarrowia lipolytica CTRG_03660 XP_002549363.1 255728875 Candida tropicalis MYA-3404 KLLA0A09339g XP_451408.1 50302941 Kluyveromyces lactis NRRL Y-1140

Phosphogluconate dehydratase

Protein GenBank ID GI Number Organism Edd AAC74921.1 1788157 Escherichia coli K-12 MG1655 Edd AAG29866.1 11095426 Zymomonas mobilis subsp. mobilis ZM4 Edd YP_350103.1 77460596 Pseudomonas fluorescens Pf0-1 ANI_1_2126094 XP_001394596.2 317032939 Aspergillus niger CBS 513.88 YALI0B15598g XP_500938.1 50546937 Yarrowia lipolytica CTRG_03660 XP_002549363.1 255728875 Candida tropicalis MYA-3404 KLLA0A09339g XP_451408.1 50302941 Kluyveromyces lactis NRRL Y-1140

2-Keto-3-deoxygluconate 6-phosphate aldolase

Protein GenBank ID GI Number Organism Eda NP_416364.1 16129803 Escherichia coli K-12 MG1655 Eda Q00384.2 59802878 Zymomonas mobilis subsp. mobilis ZM4 Eda ABA76098.1 77384585 Pseudomonas fluorescens Pf0-1

Soluble transhydrogenase

Protein GenBank ID GI Number Organism SthA NP_418397.2 90111670 Escherichia coli K-12 MG1655 SthA YP_002798658.1 226943585 Azotobacter vinelandii DJ SthA O05139.3 11135075 Pseudomonas fluorescens

Membrane-bound transhydrogenase

Protein GenBank ID GI Number Organism ANI_1_29100 XP_001400109.2 317027842 Aspergillus niger CBS 513.88 Pc21g18800 XP_002568871.1 226943585 255956237 Penicillium chrysogenum Wisconsin 54-1255 SthA O05139.3 11135075 Pseudomonas fluorescens NCU01140 XP_961047.2 164426165 Neurospora crassa OR74A

NADP-dependant glyceraldehyde-3-phosphate dehydrogenase

Protein GenBank ID GI Number Organism gapN AAA91091.1 642667 Streptococcus mutans NP-GAPDH AEC07555.1 330252461 Arabidopsis thaliana GAPN AAM77679.2 82469904 Triticum aestivum gapN CAI56300.1 87298962 Clostridium acetobutylicum NADP-GAPDH 2D2I_A 112490271 Synechococcus elongatus PCC 7942 NADP-GAPDH CAA62619.1 4741714 Synechococcus elongatus PCC 7942 GDP1 XP_455496.1 50310947 Kluyveromyces lactis NRRL Y-1140 HP1346 NP_208138.1 15645959 Helicobacter pylori 26695

NAD-dependant glyceraldehyde-3-phosphate dehydrogenase

Protein GenBank ID GI Number Organism TDH1 NP_012483.1 6322409 Saccharomyces cerevisiae s288c TDH2 NP_012542.1 6322468 Saccharomyces cerevisiae s288c TDH3 NP_011708.1 632163 Saccharomyces cerevisiae s288c KLLA0A11858g XP_451516.1 50303157 Kluyveromyces lactis NRRL Y-1140 KLLA0F20988g XP_456022.1 50311981 Kluyveromyces lactis NRRL Y-1140 ANI_1_256144 XP_001397496.1 145251966 Aspergillus niger CBS 513.88 YALI0C06369g XP_501515.1 50548091 Yarrowia lipolytica CTRG_05666 XP_002551368.1 255732890 Candida tropicalis MYA-3404

Mutated LpdA from E. coli K-12 MG1655 described in  Biochemistry, 1993, 32 (11), pp 2737-2740: (SEQ ID NO: 3) MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGVCLNVGC IPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGG LAGMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPI QLPFIPHEDPRIWDSTDALELKEVPERLLVMGGIIGLEMGTVYHALGSQI DVVVRKHQVIRAADKDIVKVFTKRISKKFNLMLETKVTAVEAKEDGIYVT MEGKKAPAEPQRYDAVLVAIGRVPNGKNLDAGKAGVEVDDRGFIRVDKQL RTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVIAGKKHYFDPKVIPSIA YTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDCADGMTKLIF DKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHES VGLAAEVFEGSITDLPNPKAKKK Mutated LpdA from E. coli K-12 MG1655 described in  Biochemistry, 1993, 32 (11), pp 2737-2740: (SEQ ID NO: 4) MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGVCLNVGC IPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGG LAGMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAHAAGSRPIQ LPFIPHEDPRIWDSTDALELKEVPERLLVMGGGIIALEMATVYHALGSQI DVVVRKHQVIRAADKDIVKVFTKRISKKFNLMLETKVTAVEAKEDGIYVT MEGKKAPAEPQRYDAVLVAIGRVPNGKNLDAGKAGVEVDDRGFIRVDKQL RTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVIAGKKHYFDPKVIPSIA YTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDCADGMTKLIF DKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHES VGLAAEVFEGSITDLPNPKAKKK

NADP-dependant formate dehydrogenase

Protein GenBank ID GI Number Organism fdh ACF35003. 194220249 Burkholderia stabilis fdh ABC20599.2 146386149 Moorella thermoacetica ATCC 39073

Mutant Candida bodinii enzyme described in Journal  of Molecular Catalysis B: Enzymatic, Volume 61,  Issues 3-4, December 2009, Pages 157-161: (SEQ ID NO: 5) MKIVLVLYDAGKHAADEEKLYGCTENKLGIANWLKDQGHELITTSDKEGE TSELDKHIPDADIIITTPFHPAYITKERLDKAKNLKLVVVAGVGSDHIDL DYINQTGKKISVLEVTGSNVVSVAEHVVMTMLVLVRNFVPAHEQIINHDW EVAAIAKDAYDIEGKTIATIGAGRIGYRVLERLLPFNPKELLYYQRQALP KEAEEKVGARRVENIEELVAQADIVTVNAPLHAGTKGLINKELLSKFKKG AWLVNTARGAICVAEDVAAALESGQLRGYGGDVWFPQPAPKDHPWRDMRN KYGAGNAMTPHYSGTTLDAQTRYAEGTKNILESFFTGKFDYRPQDIILLN GEYVTKAYGKHDKK Mutant Candida bodinii enzyme described in Journal  of Molecular Catalysis B: Enzymatic, Volume 61,  Issues 3-4, December 2009, Pages 157-161: (SEQ ID NO: 6) MKIVLVLYDAGKHAADEEKLYGCTENKLGIANWLKDQGHELITTSDKEGE TSELDKHIPDADIIITTPFHPAYITKERLDKAKNLKLVVVAGVGSDHIDL DYINQTGKKISVLEVTGSNVVSVAEHVVMTMLVLVRNFVPAHEQIINHDW EVAAIAKDAYDIEGKTIATIGAGRIGYRVLERLLPFNPKELLYYSPQALP KEAEEKVGARRVENIEELVAQADIVTVNAPLHAGTKGLINKELLSKFKKG AWLVNTARGAICVAEDVAAALESGQLRGYGGDVWFPQPAPKDHPWRDMRN KYGAGNAMTPHYSGTTLDAQTRYAEGTKNILESFFTGKFDYRPQDIILLN GEYVTKAYGKHDKK Mutant Saccharomyces cerevisiae enzyme described  in Biochem J. 2002 November 1:367(Pt 3):841-847: (SEQ ID NO: 7) MSKGKVLLVLYEGGKHAEEQEKLLGCIENELGIRNFIEEQGYELVTTIDK DPEPTSTVDRELKDAEIVITTPFFPAYISRNRIAEAPNLKLCVTAGVGSD HVDLEAANERKITVTEVTGSNVVSVAEHVMATILVLIRNYNGGHQQAING EWDIAGVAKNEYDLEDKIISTVGAGRIGYRVLERLVAFNPKKLLYYARQE LPAEAINRLNEASKLFNGRGDIVQRVEKLEDMVAQSDVVTINCPLHKDSR GLFNKKLISHMKDGAYLVNTARGAICVAEDVAEAVKSGKLAGYGGDVWDK QPAPKDHPWRTMDNKDHVGNAMTVHISGTSLDAQKRYAQGVKNILNSYFS KKFDYRPQDIIVQNGSYATRAYGQKK.

NADPH:ferredoxin oxidoreductase

Protein GenBank ID GI Number Organism petH YP_171276.1 56750575 Synechococcus elongatus PCC 6301 fpr NP_457968.1 16762351 Salmonella enterica fnr1 XP_001697352.1 159478523 Chlamydomonas reinhardtii rfnr1 NP_567293.1 18412939 Arabidopsis thaliana aceF NP_414657.1 6128108 Escherichia coli K-12 MG1655

NADP-dependant acylating acetylaldehyde dehydrogenase

Protein GenBank ID GI Number Organism adhB AAB06720.1 1513071 Thermoanaerobacter pseudethanolicus ATCC 33223 TheetDRAFT_0840 ZP_08211603. 326390041 Thermoanaerobacter ethanolicus JW 200 Cbei_3832 YP_001310903.1 150018649 Clostridium beijerinckii NCIMB 8052 Cbei_4054 YP_001311120.1 150018866 Clostridium beijerinckii NCIMB 8052 Cbei_4045 YP_001311111.1 150018857 Clostridium beijerinckii NCIMB 8052

Exemplary genes encoding pyruvate dehydrogenase, pyruvate:ferredoxin oxidoreductase, pyruvate formate lyase, pyruvate decarboxylase, acetate kinase, phosphotransacetylase and acetyl-CoA synthetase are described above in Example V.

Example XI Engineering Saccharomyces cerevisiae for Chemical Production

Eukaryotic hosts have several advantages over prokaryotic systems. They are able to support post-translational modifications and host membrane-anchored and organelle-specific enzymes. Genes in eukaryotes typically have introns, which can impact the timing of gene expression and protein structure.

An exemplary eukaryotic organism well suited for industrial chemical production is Saccharomyces cerevisiae. This organism is well characterized, genetically tractable and industrially robust Genes can be readily inserted, deleted, replaced, overexpressed or underexpressed using methods known in the art. Some methods are plasmid-based whereas others modify the chromosome (Guthrie and Fink. Guide to Yeast Genetics and Molecular and Cell Biology, Part B, Volume 350, Academic Press (2002); Guthrie and Fink, Guide to Yeast Genetics and Molecular and Cell Biology, Part C, Volume 351, Academic Press (2002)).

Plasmid-mediated gene expression is enabled by yeast episomal plasmids (YEps). YEps allow for high levels of expression; however they are not very stable and they require cultivation in selective media. They also have a high maintenance cost to the host metabolism. High copy number plasmids using auxotrophic (e.g., URA3, TRP1, HIS3, LEU2) or antibiotic selectable markers (e.g., Zeo^(R) or Kan^(R)) can be used, often with strong, constitutive promoters such as PGK1 or ACT1 and a transcription terminator-polyadenylation region such as those from CYC1 or AOX. Many examples are available for one well-versed in the art. These include pVV214 (a 2 micron plasmid with URA3 selectable marker) and pVV200 (2 micron plasmid with TRP1 selectable marker) (Van et al., Yeast 20:739-746 (2003)). Alternatively, low copy plasmids such as centromeric or CEN plamids can be used. Again, many examples are available for one well-versed in the art. These include pRS313 and pRS315 (Sikorski and Hieter, Genetics 122:19-27 (1989) both of which require that a promoter (e.g., PGK1 or ACT1) and a terminator (e.g., CYC1, AOX) are added.

For industrial applications, chromosomal overexpression of genes is preferable to plasmid-mediated overexpression. Mikkelsen and coworkers have identified 11 integration sites on highly expressed regions of the S. cerevisiae genome on chromosomes X, XI and XII (Mikkelsen et al, Met Eng 14:104-11 (2012)). The sites are separated by essential genes, minimizing the possibility of recombination between sites.

Tools for inserting genes into eukaryotic organisms such as S. cerevisiae are known in the art Particularly useful tools include yeast integrative plasmids (YIps), yeast artificial chromosomes (YACS) and gene targeting/homologous recombination. Note that these tools can also be used to insert, delete, replace, underexpress or otherwise alter the genome of the host.

Yeast integrative plasmids (YIps) utilize the native yeast homologous recombination system to efficiently integrate DNA into the chromosome. These plasmids do not contain an origin of replication and can therefore only be maintained after chromosomal integration. An exemplary construct includes a promoter, the gene of interest, a terminator, and a selectable marker with a promoter, flanked by FRT sites, loxP sites, or direct repeats enabling the removal and recycling of the resistance marker. The method entails the synthesis and amplification of the gene of interest with suitable primers, followed by the digestion of the gene at a unique restriction site, such as that created by the EcoRI and)(ha enzymes (Vellanki et al., Biotechnol Lett. 29:313-318 (2007)). The gene of interest is inserted at the EcoRI and)(ha sites into a suitable expression vector, downstream of the promoter. The gene insertion is verified by PCR and DNA sequence analysis. The recombinant plasmid is then linearized and integrated at a desired site into the chromosomal DNA of S. cerevisiae using an appropriate transformation method. The cells are plated on the YPD medium with an appropriate selection marker and incubated for 2-3 days. The transformants are analyzed for the requisite gene insert by colony PCR To remove the antibiotic marker from a construct flanked by loxP sites, a plasmid containing the Cre recombinase is introduced. Cre recombinase promotes the excision of sequences flanked by loxP sites. (Gueldener et al., Nucleic Acids Res 30:e23 (2002)). The resulting strain is cured of the Cre plasmid by successive culturing on media without any antibiotic present. Alternately, the Cre recombinase plasmid has a URA selection marker and the plasmid is efficiently removed by growing cells on 5-FOA which acts as a counter-selection for URA. This method can also be employed for a starless integration instead of using loxP. One skilled in the art can integrate using URA as a marker, select for integration by growing on URA-minus plates, and then select for URA mutants by growing on 5-FOA plates. 5-FOA is converted to the toxic 5-fluoruracil by the URA gene product. Alternatively, the FLP-FRT system can be used to integrate genes into the chromosome. This system involves the recombination of sequences between short Flipase Recognition Target (FRT) sites by the Flipase recombination enzyme (FLP) derived from the 2μ plasmid of the yeast Saccharomyces cerevisiae (Sadowski, P. D., Prog. Nucleic. Acid. Res. Mol. Biol. 51:53-91 (1995); Zhu and Sadowski J. Biol. Chem. 270:23044-23054 (1995)). Similarly, gene deletion methodologies will be carried out as described in refs. Baudin et al. Nucleic. Acids Res. 21:3329-3330 (1993); Brachmann et al., Yeast 14:115-132 (1998); Giaever et al., Nature 418:387-391 (2002); Longtine et al., Yeast 14:953-961 (1998) Winzeler et al., Science 285:901-906 (1999).

Another approach for manipulating the yeast chromosome is gene targeting. This approach takes advantage of the fact that double stranded DNA breaks in yeast are repaired by homologous recombination. Linear DNA fragments flanked by targeting sequences can thus be efficiently integrated into the yeast genome using the native homologous recombination machinery. In addition to the application of inserting genes, gene targeting approaches are useful for genomic DNA manipulations such as deleting genes, introducing mutations in a gene, its promoter or other regulatory elements, or adding a tag to a gene.

Yeast artificial chromosomes (YACs) are artificial chromosomes useful for pathway construction and assembly. YACs enable the expression of large sequences of DNA (100-3000 kB) containing multiple genes. The use of YACs was recently applied to engineer flavenoid biosynthesis in yeast (Naesby et al, Microb Cell Fact 8:49-56 (2009)). In this approach, YACs were used to rapidly test randomly assembled pathway genes to find the best combination.

The expression level of a gene can be modulated by altering the sequence of a gene and/or its regulatory regions. Such gene regulatory regions include, for example, promoters, enhancers, introns, and terminators. Functional disruption of negative regulatory elements such as repressors and/or silencers also can be employed to enhance gene expression. RNA based tools can also be employed to regulate gene expression. Such tools include RNA aptamers, riboswitches, antisense RNA, ribozymes and riboswitches.

For altering a gene's expression by its promoter, libraries of constitutive and inducible promoters of varying strengths are available. Strong constitutive promoters include pTEF1, pADH1 and promoters derived from glycolytic pathway genes. The pGAL promoters are well-studied inducible promoters activated by galactose and repressed by glucose. Another commonly used inducible promoter is the copper inducible promoter pCUP1 (Farhi et al, Met Eng 13:474-81 (2011)). Further variation of promoter strengths can be introduced by mutagenesis or shuffling methods. For example, error prone PCR can be applied to generate synthetic promoter libraries as shown by Alper and colleagues (Alper et al, PNAS 102:12678-83 (2005)). Promoter strength can be characterized by reporter proteins such as beta-galactosidase, fluorescent proteins and luciferase.

The placement of an inserted gene in the genome can alter its expression level. For example, overexpression of an integrated gene can be achieved by integrating the gene into repeating DNA elements such as ribosomal DNA or long terminal repeats.

For exogenous expression in yeast or other eukaryotic cells, genes can be expressed in the cytosol without the addition of leader sequence, or can be targeted to mitochondrion or other organelles, or targeted for secretion, by the addition of a suitable targeting sequence such as a mitochondrial targeting or secretion signal suitable for the host cells. Thus, it is understood that appropriate modifications to a nucleic acid sequence to remove or include a targeting sequence can be incorporated into an exogenous nucleic acid sequence to impart desirable properties. Genetic modifications can also be made to enhance polypeptide synthesis. For example, translation efficiency is enhanced by substituting ribosome binding sites with an optimal or consensus sequence and/or altering the sequence of a gene to add or remove secondary structures. The rate of translation can also be increased by substituting one coding sequence with another to better match the codon preference of the host.

Example XII Termination Pathways for Making Fatty Alcohols, Aldehydes and Acids

This example describes enzymes for converting intermediates of the MI-FAE cycle or MD-FAE cycle to products of interest such as fatty alcohols, fatty aldehydes, and fatty acids. Pathways are shown in FIGS. 2 and 8. Enzymes for catalyzing steps A-G are disclosed in Example IV. This example describes enzymes suitable for catalyzing steps H-N.

Enzymes include: A. Thiolase, B. 3-Ketoacyl-CoA reductase, C. 13-Hydroxyl-ACP dehydratase, D. Enoyl-CoA reductase, E. Acyl-CoA reductase (aldehyde forming), F. Alcohol dehydrogenase, G. Acyl-CoA reductase (alcohol forming), H. acyl-CoA hydrolase, transferase or synthetase, J. Acyl-ACP reductase, K. Acyl-CoA:ACP acyltransferase, L. Thioesterase, N. Aldehyde dehydrogenase (acid forming) or carboxylic acid reductase.

Pathways for converting an MI-FAE cycle intermediate to an fatty alcohol, fatty aldehyde or fatty acid product are shown in the table below. These pathways are also referred to herein as “termination pathways”.

Product Termination pathway enzymes from FIG. 2 Acid H K/L E/N K/J/N Aldehyde E K/J H/N K/L/N Alcohol E/F K/J/F H/N/F K/L/N/F G

Product specificity can be fine-tuned using one or more enzymes shown in FIGS. 2 and 7. Chain length is controlled by one or more enzymes of the elongation pathway in conjunction with one more enzymes of the termination pathway as described above. The structure of the product is controlled by one or more enzymes of the termination pathway. Examples of selected termination pathway enzymes reacting with various pathway intermediates are shown in the table below. Additional examples are described herein.

Enzyme Substrate Example Acyl-CoA reductase Acyl-CoA Acr1 of A. bayliyi (GenBank AAC45217) 3-Hydroxyacyl-CoA PduP of L. reuteri (GenBank CCC03595.1) 3-Oxoacyl-CoA Mcr of S. tokodaii (GenBank NP_378167) Acyl-CoA hydrolase, Acyl-CoA tesB of E. coli (GenBank NP_414986) transferase or synthetase 3-Hydroxyacyl-CoA hibch of R. norvegicus (GenBank Q5XIE6.2) 3-Oxoacyl-CoA MKS2 of S. lycopersicum (GenBank ACG69783) Enoyl-CoA gctAB of Acidaminococcus fermentans (GenBank CAA57199, CAA57200) Acyl-ACP acyltransferase Acyl-CoA fabH of E. coli (GenBank AAC74175.1)

Step H. Acyl-CoA Hydrolase, Transferase or Synthase

Acyl-CoA hydrolase, transferase and synthase enzymes convert acyl-CoA moieties to their corresponding acids. Such an enzyme can be utilized to convert, for example, a fatty acyl-CoA to a fatty acid, a 3-hydroxyacyl-CoA to a 3-hydroxyacid, a 3-oxoacyl-CoA to a 3-oxoacid, or an enoyl-CoA to an enoic acid.

CoA hydrolase or thioesterase enzymes in the 3.1.2 family hydrolyze acyl-CoA molecules to their corresponding acids. Several CoA hydrolases with different substrate ranges are suitable for hydrolyzing acyl-CoA, 3-hydroxyacyl-CoA, 3-oxoacyl-CoA and enoyl-CoA substrates to their corresponding acids. For example, the enzyme encoded by acot12 from Rattus norvegicus brain (Robinson et al., Biochem. Biophys. Res. Commun. 71:959-965 (1976)) can react with butyryl-CoA, hexanoyl-CoA and malonyl-CoA. The human dicarboxylic acid thioesterase, encoded by acot8, exhibits activity on glutaryl-CoA, adipyl-CoA, suberyl-CoA, sebacyl-CoA, and dodecanedioyl-CoA (Westin et al., J. Biol. Chem. 280:38125-38132 (2005)). The closest E. coli homolog to this enzyme, tesB, can also hydrolyze a range of CoA thiolesters (Naggert et al., J Biol Chem 266:11044-11050 (1991)). A similar enzyme has also been characterized in the rat liver (Deana R., Biochem Int 26:767-773 (1992)). Additional enzymes with hydrolase activity in E. coli include ybgC, paaI, and ybdB (Kuznetsova, et al., FEMS Microbiol Rev, 2005, 29(2):263-279; Song et al., J Biol Chem, 2006, 281(16):11028-38). Though its sequence has not been reported, the enzyme from the mitochondrion of the pea leaf has a broad substrate specificity, with demonstrated activity on acetyl-CoA, propionyl-CoA, butyryl-CoA, palmitoyl-CoA, oleoyl-CoA, succinyl-CoA, and crotonyl-CoA (Zeiher et al., Plant. Physiol. 94:20-27 (1990)) The acetyl-CoA hydrolase, ACH1, from S. cerevisiae represents another candidate hydrolase (Buu et al., J. Biol. Chem. 278:17203-17209 (2003)). Additional enzymes with aryl-CoA hydrolase activity include the palmitoyl-CoA hydrolase of Mycobacterium tuberculosis (Wang et al., Chem. Biol. 14:543-551 (2007)) and the acyl-CoA hydrolase of E. coli encoded by entH (Guo et al., Biochemistry 48:1712-1722 (2009)). Additional CoA hydrolase enzymes are described above.

Gene name GenBank Accession # GI# Organism acot12 NP_570103.1 18543355 Rattus norvegicus tesB NP_414986 16128437 Escherichia coli acot8 CAA15502 3191970 Homo sapiens acot8 NP_570112 51036669 Rattus norvegicus tesA NP_415027 16128478 Escherichia coli ybgC NP_415264 16128711 Escherichia coli paaI NP_415914 16129357 Escherichia coli ybdB NP_415129 16128580 Escherichia coli ACH1 NP_009538 6319456 Saccharomyces cerevisiae Rv0098 NP_214612.1 15607240 Mycobacterium tuberculosis entH AAC73698.1 1786813 Escherichia coli

CoA hydrolase enzymes active on 3-hydroxyacyl-CoA, 3-oxoacyl-CoA and enoyl-CoA intermediates are also well known in the art. For example, an enzyme for converting enoyl-CoA substrates to their corresponding acids is the glutaconate CoA-transferase from Acidaminococcus fermentans. This enzyme was transformed by site-directed mutagenesis into an acyl-CoA hydrolase with activity on glutaryl-CoA, acetyl-CoA and 3-butenoyl-CoA (Mack et al., FEBS. Lett. 405:209-212 (1997)). Another suitable enzyme is the fadM thioesterase III of E. coli. This enzyme is involved in oleate beta-oxidation and the preferred substrate is 3,5-tetradecadienoyl-CoA (Nie et al, Biochem 47:7744-51 (2008)).

Protein GenBank ID GI Number Organism gctA CAA57199.1 559392 Acidaminococcus fermentans gctB CAA57200.1 559393 Acidaminococcus fermentans gctA ACJ24333.1 212292816 Clostridium symbiosum gctB ACJ24326.1 212292808 Clostridium symbiosum gctA NP_603109.1 19703547 Fusobacterium nucleatum gctB NP_603110.1 19703548 Fusobacterium nucleatum fadM NP_414977.1 16128428 Escherichia coli

3-Hydroxyisobutyryl-CoA hydrolase is active on 3-hydroxyacyl-CoA substrates (Shimomura et al., J Biol Chem. 269:14248-14253 (1994)). Genes encoding this enzyme include hibch of Rattus norvegicus (Shimomura et al., Methods Enzymol. 324:229-240 (2000)) and Homo sapiens (Shimomura et al., supra). Similar gene candidates can also be identified by sequence homology, including hibch of Saccharomyces cerevisiae and BC 2292 of Bacillus cereus. An exemplary 3-oxoacyl-CoA hydrolase is MKS2 of Solanum lycopersicum (Yu et al, Plant Physiol 154:67-77 (2010)). The native substrate of this enzyme is 3-oxo-myristoyl-CoA, which produces a C14 chain length product.

GenBank Gene name Accession # GI# Organism fadM NP_414977.1 16128428 Escherichia coli hibch Q5XIE6.2 146324906 Rattus norvegicus hibch Q6NVY1.2 146324905 Homo sapiens hibch P28817.2 2506374 Saccharomyces cerevisiae BC_2292 AP09256 29895975 Bacillus cereus MKS2 ACG69783.1 196122243 Solanum lycopersicum

CoA transferases catalyze the reversible transfer of a CoA moiety from one molecule to another. Several transformations require a CoA transferase to activate carboxylic acids to their corresponding acyl-CoA derivatives. CoA transferase enzymes have been described in the open literature and represent suitable candidates for these steps. These are described below.

The gene products of cat1, cat2, and cat3 of Clostridium kluyveri have been shown to exhibit succinyl-CoA, 4-hydroxybutyryl-CoA, and butyryl-CoA transferase activity, respectively (Seedorf et al., Proc. Natl. Acad. Sci U.S.A 105:2128-2133 (2008); Sohling et al., J Bacteriol. 178:871-880 (1996)) Similar CoA transferase activities are also present in Trichomonas vaginalis, Trypanosoma brucei, Clostridium aminobutyricum and Porphyromonas gingivalis (Riviere et al., J. Biol. Chem. 279:45337-45346 (2004); van Grinsven et al., J. Biol. Chem. 283:1411-1418 (2008)).

Protein GenBank ID GI Number Organism cat1 P38946.1 729048 Clostridium kluyveri cat2 P38942.2 172046066 Clostridium kluyveri cat3 EDK35586.1 146349050 Clostridium kluyveri TVAG_395550 XP_001330176 123975034 Trichomonas vaginalis G3 Tb11.02.0290 XP_828352 71754875 Trypanosoma brucei cat2 CAB60036.1 6249316 Clostridium aminobutyricum cat2 NP_906037.1 34541558 Porphyromonas gingivalis W83

A fatty acyl-CoA transferase that utilizes acetyl-CoA as the CoA donor is acetoacetyl-CoA transferase, encoded by the E. coli atoA (alpha subunit) and atoD (beta subunit) genes (Korolev et al., Acta Crystallogr. D. Biol. Crystallogr. 58:2116-2121 (2002); Vanderwinkel et al., 33:902-908 (1968)). This enzyme has a broad substrate range on substrates of chain length C3-C6 (Sramek et al., Arch Biochem Biophys 171:14-26 (1975)) and has been shown to transfer the CoA moiety to acetate from a variety of branched and linear 3-oxo and acyl-CoA substrates, including isobutyrate (Matthies et al., Appl Environ. Microbiol 58:1435-1439 (1992)), valerate (Vanderwinkel et al., Biochem. Biophys. Res. Commun. 33:902-908 (1968)) and butanoate (Vanderwinkel et al., Biochem. Biophys. Res. Commun. 33:902-908 (1968)). This enzyme is induced at the transcriptional level by acetoacetate, so modification of regulatory control may be necessary for engineering this enzyme into a pathway (Pauli et al., Eur. J Biochem. 29:553-562 (1972)) Similar enzymes exist in Corynebacterium glutamicum ATCC 13032 (Duncan et al., 68:5186-5190 (2002)), Clostridium acetobutylicum (Cary et al., Appl Environ Microbiol 56:1576-1583 (1990); Wiesenborn et al., Appl Environ Microbiol 55:323-329 (1989)), and Clostridium saccharoperbutylacetonicum (Kosaka et al., Biosci. Biotechnol Biochem. 71:58-68 (2007)).

Gene GI # Accession No. Organism atoA 2492994 P76459.1 Escherichia coli atoD 2492990 P76458.1 Escherichia coli actA 62391407 YP_226809.1 Corynebacterium glutamicum cg0592 62389399 YP_224801.1 Corynebacterium glutamicum ctfA 15004866 NP_149326.1 Clostridium acetobutylicum ctfB 15004867 NP_149327.1 Clostridium acetobutylicum ctfA 31075384 AAP42564.1 Clostridium saccharoperbutylacetonicum ctfB 31075385 AAP42565.1 Clostridium saccharoperbutylacetonicum

Beta-ketoadipyl-CoA transferase, also known as succinyl-CoA:3:oxoacid-CoA transferase, is active on 3-oxoacyl-CoA substrates. This enzyme is encoded by pcaI and pcaJ in Pseudomonas putida (Kaschabek et al., J Bacteriol. 184:207-215 (2002)). Similar enzymes are found in Acinetobacter sp. ADP1 (Kowalchuk et al., Gene 146:23-30 (1994)), Streptomyces coelicolor and Pseudomonas knackmussii (formerly sp. B13) (Gobel et al., J Bacteriol. 184:216-223 (2002); Kaschabek et al., J Bacteriol. 184:207-215 (2002)). Additional exemplary succinyl-CoA:3:oxoacid-CoA transferases have been characterized in Helicobacter pylori (Corthesy-Theulaz et al., J Biol. Chem. 272:25659-25667 (1997)), Bacillus subtilis (Stols et al., Protein Expr. Purif. 53:396-403 (2007)) and Homo sapiens (Fukao, T., et al., Genomics 68:144-151 (2000); Tanaka, H., et al., Mol Hum Reprod 8:16-23 (2002)). Genbank information related to these genes is summarized below.

Gene GI # Accession No. Organism pcaI 24985644 AAN69545.1 Pseudomonas putida pcaJ 26990657 NP_746082.1 Pseudomonas putida pcaI 50084858 YP_046368.1 Acinetobacter sp. ADP1 pcaJ 141776 AAC37147.1 Acinetobacter sp. ADP1 pcaI 21224997 NP_630776.1 Streptomyces coelicolor pcaJ 21224996 NP_630775.1 Streptomyces coelicolor catI 75404583 Q8VPF3 Pseudomonas knackmussii catJ 75404582 Q8VPF2 Pseudomonas knackmussii HPAG1_0676 108563101 YP_627417 Helicobacter pylori HPAG1_0677 108563102 YP_627418 Helicobacter pylori ScoA 16080950 NP_391778 Bacillus subtilis ScoB 16080949 NP_391777 Bacillus subtilis OXCT1 NP_000427 4557817 Homo sapiens OXCT2 NP_071403 11545841 Homo sapiens

The conversion of acyl-CoA substrates to their acid products can be catalyzed by a CoA acid-thiol ligase or CoA synthetase in the 6.2.1 family of enzymes. CoA synthases that convert ATP to ADP (ADP-forming) are reversible and react in the direction of acid formation, whereas AMP forming enzymes only catalyze the activation of an acid to an acyl-CoA. For fatty acid formation, deletion or attenuation of AMP forming enzymes will reduce backflux. ADP-forming acetyl-CoA synthetase (ACD, EC 6.2.1.13) is an enzyme that couples the conversion of acyl-CoA esters to their corresponding acids with the concomitant synthesis of ATP. ACD I from Archaeoglobus fulgidus, encoded by AF1211, was shown to operate on a variety of linear and branched-chain substrates including isobutyrate, isopentanoate, and fumarate (Musfeldt et al., J Bacteriol. 184:636-644 (2002)). A second reversible ACD in Archaeoglobus fulgidus, encoded by AF1983, was also shown to have a broad substrate range (Musfeldt and Schonheit, J Bacteriol. 184:636-644 (2002)). The enzyme from Haloarcula marismortui (annotated as a succinyl-CoA synthetase) accepts propionate, butyrate, and branched-chain acids (isovalerate and isobutyrate) as substrates, and was shown to operate in the forward and reverse directions (Brasen et al., Arch Microbiol 182:277-287 (2004)). The ACD encoded by PAE3250 from hyperthermophilic crenarchaeon Pyrobaculum aerophilum showed the broadest substrate range of all characterized ACDs, reacting with acetyl-CoA, isobutyryl-CoA (preferred substrate) and phenylacetyl-CoA (Brasen et al, supra). Directed evolution or engineering can be used to modify this enzyme to operate at the physiological temperature of the host organism. The enzymes from A. fulgidus, H. marismortui and P. aerophilum have all been cloned, functionally expressed, and characterized in E. coli (Brasen and Schonheit, supra; Musfeldt and Schonheit, J Bacteriol. 184:636-644 (2002)). An additional candidate is succinyl-CoA synthetase, encoded by sucCD of E. coli and LSC1 and LSC2 genes of Saccharomyces cerevisiae. These enzymes catalyze the formation of succinyl-CoA from succinate with the concomitant consumption of one ATP in a reaction which is reversible in vivo (Buck et al., Biochemistry 24:6245-6252 (1985)). The acyl CoA ligase from Pseudomonas putida has been demonstrated to work on several aliphatic substrates including acetic, propionic, butyric, valeric, hexanoic, heptanoic, and octanoic acids and on aromatic compounds such as phenylacetic and phenoxyacetic acids (Fernandez-Valverde et al., Appl. Environ. Microbiol. 59:1149-1154 (1993)). A related enzyme, malonyl CoA synthetase (6.3.4.9) from Rhizobium leguminosarum could convert several diacids, namely, ethyl-, propyl-, allyl-, isopropyl-, dimethyl-, cyclopropyl-, cyclopropylmethylene-, cyclobutyl-, and benzyl-malonate into their corresponding monothioesters (Pohl et al., J. Am. Chem. Soc. 123:5822-5823 (2001)).

Protein GenBank ID GI Number Organism AF1211 NP_070039.1 11498810 Archaeoglobus fulgidus AF1983 NP_070807.1 11499565 Archaeoglobus fulgidus scs YP_135572.1 55377722 Haloarcula marismortui PAE3250 NP_560604.1 18313937 Pyrobaculum aerophilum str. IM2 sucC NP_415256.1 16128703 Escherichia coli sucD AAC73823.1 1786949 Escherichia coli LSC1 NP_014785 6324716 Saccharomyces cerevisiae LSC2 NP_011760 6321683 Saccharomyces cerevisiae paaF AAC24333.2 22711873 Pseudomonas putida matB AAC83455.1 3982573 Rhizobium leguminosarum

Step J. Acyl-ACP Reductase

The reduction of an acyl-ACP to its corresponding aldehyde is catalyzed by an acyl-ACP reductase (AAR). Such a transformation is depicted in step J of FIGS. 2 and 8. Suitable enzyme candidates include the orf1594 gene product of Synechococcus elongatus PCC7942 and homologs thereof (Schirmer et al, Science, 329: 559-62 (2010)). The S. elongates PCC7942 acyl-ACP reductase is coexpressed with an aldehyde decarbonylase in an operon that appears to be conserved in a majority of cyanobacterial organisms. This enzyme, expressed in E. coli together with the aldehyde decarbonylase, conferred the ability to produce alkanes. The P. marinus AAR was also cloned into E. coli and, together with a decarbonylase, demonstrated to produce alkanes (US Application 2011/0207203).

Protein GenBank ID GI Number Organism orf1594 YP_400611.1 81300403 Synechococcus elongatus PCC7942 PMT9312_0533 YP_397030.1 78778918 Prochlorococcus marinus MIT 9312 syc0051_d YP_170761.1 56750060 Synechococcus elongatus PCC 6301 Ava_2534 YP_323044.1 75908748 Anabaena variabilis ATCC 29413 alr5284 NP_489324.1 17232776 Nostoc sp. PCC 7120 Aazo_3370 YP_003722151.1 298491974 Nostoc azollae Cyan7425_0399 YP_002481152.1 220905841 Cyanothece sp. PCC 7425 N9414_21225 ZP_01628095.1 119508943 Nodularia spumigena CCY9414 L8106_07064 ZP_01619574.1 119485189 Lyngbya sp. PCC 8106

Step K. Acyl-CoA:ACP Acyltransferase

The transfer of an acyl-CoA to an acyl-ACP is catalyzed by acyltransferase enzymes in EC class 2.3.1. Enzymes with this activity are described above.

Step L. Thioesterase

Acyl-ACP thioesterase enzymes convert an acyl-ACP to its corresponding acid. Such a transformation is required in step L of FIG. 2. Exemplary enzymes include the FatA and FatB isoforms of Arabidopsis thaliana (Salas et al, Arch Biochem Biophys 403:25-34 (2002)). The activities of these two proteins vary with carbon chain length, with FatA preferring oleyl-ACP and FatB preferring palmitoyl-ACP. A number of thioesterases with different chain length specificities are listed in WO 2008/113041 and are included in the table below. For example, it has been shown previously that expression of medium chain plant thioesterases like FatB from Umbellularia californica in E. coli results in accumulation of high levels of medium chain fatty acids, primarily laurate (C12:0). Similarly, expression of Cuphea palustris FatB1 thioesterase in E. coli led to accumulation of C8-10:0 products (Dehesh et al, Plant Physiol 110:203-10 (1996)). Similarly, Carthamus tinctorius thioesterase expressed in E. coli leads to >50 fold elevation in C 18:1 chain termination and release as free fatty acid (Knutzon et al, Plant Physiol 100:1751-58 (1992)). Methods for altering the substrate specificity of thioesterases are also known in the art (for example, EP 1605048).

Protein GenBank ID GI Number Organism fatA AEE76980.1 332643459 Arabidopsis thaliana fatB AEE28300.1 332190179 Arabidopsis thaliana fatB2 AAC49269.1 1292906 Cuphea hookeriana fatB3 AAC72881.1 3859828 Cuphea hookeriana fatB1 AAC49179.1 1215718 Cuphea palustris M96568.1:94..1251 AAA33019.1 404026 Carthamus tinctorius fatB1 Q41635.1 8469218 Umbellularia californica tesA AAC73596.1 1786702 Escherichia coli

Step N. Aldehyde Dehydrogenase (Acid Forming) or Carboxylic Acid Reductase

The conversion of an aldehyde to an acid is catalyzed by an acid-forming aldehyde dehydrogenase. Several Saccharomyces cerevisiae enzymes catalyze the oxidation of aldehydes to acids including ALD1 (ALD6), ALD2 and ALD3 (Navarro-Avino et al, Yeast 15:829-42 (1999); Quash et al, Biochem Pharmacol 64:1279-92 (2002)). The mitochondrial proteins ALD4 and ALD5 catalyze similar transformations (Wang et al, J Bacteriol 180:822-30 (1998); Boubekeur et al, Eur J Biochem 268:5057-65 (2001)). HFD1 encodes a hexadecanal dehydrogenase. Exemplary acid-forming aldehyde dehydrogenase enzymes are listed in the table below.

Protein GenBank ID GI number Organism ALD2 NP_013893.1 6323822 Saccharomyces cerevisiae s288c ALD3 NP_013892.1 6323821 Saccharomyces cerevisiae s288c ALD4 NP_015019.1 6324950 Saccharomyces cerevisiae s288c ALD5 NP_010996.2 330443526 Saccharomyces cerevisiae s288c ALD6 NP_015264.1 6325196 Saccharomyces cerevisiae s288c HFD1 NP_013828.1 6323757 Saccharomyces cerevisiae s288c CaO19.8361 XP_710976.1 68490403 Candida albicans CaO19.742 XP_710989.1 68490378 Candida albicans YALI0C03025 CAG81682.1 49647250 Yarrowia lipolytica ANI_1_1334164 XP_001398871.1 145255133 Aspergillus niger ANI_1_2234074 XP_001392964.2 317031176 Aspergillus niger ANI_1_226174 XP_001402476.1 145256256 Aspergillus niger ALDH P41751.1 1169291 Aspergillus niger KLLA0D09999 CAH00602.1 49642640 Kluyveromyces lactis

The conversion of an acid to an aldehyde is thermodynamically unfavorable and typically requires energy-rich cofactors and multiple enzymatic steps. For example, in butanol biosynthesis conversion of butyrate to butyraldehyde is catalyzed by activation of butyrate to its corresponding acyl-CoA by a CoA transferase or ligase, followed by reduction to butyraldehyde by a CoA-dependent aldehyde dehydrogenase. Alternately, an acid can be activated to an acyl-phosphate and subsequently reduced by a phosphate reductase. Direct conversion of the acid to aldehyde by a single enzyme is catalyzed by a bifunctional carboxylic acid reductase enzyme in the 1.2.1 family. Exemplary enzymes that catalyze these transformations include carboxylic acid reductase, alpha-aminoadipate reductase and retinoic acid reductase.

Carboxylic acid reductase (CAR), found in Nocardia iowensis, catalyzes the magnesium, ATP and NADPH-dependent reduction of carboxylic acids to their corresponding aldehydes (Venkitasubramanian et al., J Biol. Chem. 282:478-485 (2007)). The natural substrate of this enzyme is benzoic acid and the enzyme exhibits broad acceptance of aromatic and aliphatic substrates including fatty acids of length C12-C18 (Venkitasubramanian et al., Biocatalysis in Pharmaceutical and Biotechnology Industries. CRC press (2006); WO 2010/135624). CAR requires post-translational activation by a phosphopantetheine transferase (PPTase) that converts the inactive apo-enzyme to the active holo-enzyme (Hansen et al., Appl. Environ. Microbiol 75:2765-2774 (2009)). The Nocardia CAR enzyme was cloned and functionally expressed in E. coli (Venkitasubramanian et al., J. Biol. Chem. 282:478-485 (2007)). Co-expression of the npt gene, encoding a specific PPTase, improved activity of the enzyme. A related enzyme from Mycobacterium sp. strain JLS catalyzes the reduction of fatty acids of length C12-C16. Variants of this enzyme with enhanced activity on fatty acids are described in WO 2010/135624. Alpha-aminoadipate reductase (AAR, EC 1.2.1.31), participates in lysine biosynthesis pathways in some fungal species. This enzyme naturally reduces alpha-aminoadipate to alpha-aminoadipate semialdehyde. The carboxyl group is first activated through the ATP-dependent formation of an adenylate that is then reduced by NAD(P)H to yield the aldehyde and AMP. Like CAR, this enzyme utilizes magnesium and requires activation by a PPTase Enzyme candidates for AAR and its corresponding PPTase are found in Saccharomyces cerevisiae (Morris et al., Gene 98:141-145 (1991)), Candida albicans (Guo et al., Mol. Genet. Genomics 269:271-279 (2003)), and Schizosaccharomyces pombe (Ford et al., Curr. Genet. 28:131-137 (1995)). The AAR from S. pombe exhibited significant activity when expressed in E. coli (Guo et al., Yeast 21:1279-1288 (2004)). The AAR from Penicillium chrysogenum accepts S-carboxymethyl-L-cysteine as an alternate substrate, but did not react with adipate, L-glutamate or diaminopimelate (Hijarrubia et al., J Biol. Chem. 278:8250-8256 (2003)). The gene encoding the P. chrysogenum PPTase has not been identified to date and no high-confidence hits were identified by sequence comparison homology searching.

Protein GenBank ID GI Number Organism car AAR91681.1 40796035 Nocardia iowensis npt ABI83656.1 114848891 Nocardia iowensis car YP_001070587.1 126434896 Mycobacterium sp. strain JLS npt YP_001070355.1 126434664 Mycobacterium sp. strain JLS LYS2 AAA34747.1 171867 Saccharomyces cerevisiae LYS5 P50113.1 1708896 Saccharomyces cerevisiae LYS2 AAC02241.1 2853226 Candida albicans LYS5 AAO26020.1 28136195 Candida albicans Lys1p P40976.3 13124791 Schizosaccharomyces pombe Lys7p Q10474.1 1723561 Schizosaccharomyces pombe Lys2 CAA74300.1 3282044 Penicillium chrysogenum

Additional car and npt genes can be identified based on sequence homology.

GenBank Gene name GI No. Accession No. Organism fadD9 121638475 YP_978699.1 Mycobacterium bovis BCG BCG_2812c 121638674 YP_978898.1 Mycobacterium bovis BCG nfa20150  54023983 YP_118225.1 Nocardia farcinica IFM 10152 nfa40540  54026024 YP_120266.1 Nocardia farcinica IFM 10152 SGR_6790 YP_001828302.1 182440583 Streptomyces griseus subsp. griseus NBRC 13350 SGR_665 YP_001822177.1 182434458 Streptomyces griseus subsp. griseus NBRC 13350 MSMEG_2956 YP_887275.1 118473501 Mycobacterium smegmatis MC2 155 MSMEG_5739 YP_889972.1 118469671 Mycobacterium smegmatis MC2 155 MSMEG_2648 YP_886985.1 118471293 Mycobacterium smegmatis MC2 155 MAP1040c NP_959974.1 41407138 Mycobacterium avium subsp. paratuberculosis K-10 MAP2899c NP_961833.1 41408997 Mycobacterium avium subsp. paratuberculosis K-10 MMAR_2117 YP_001850422.1 183982131 Mycobacterium marinum M MMAR_2936 YP_001851230.1 183982939 Mycobacterium marinum M MMAR_1916 YP_001850220.1 183981929 Mycobacterium marinum M Tpau_1373 YP_003646340.1 296139097 Tsukamurella paurometabola DSM 20162 Tpau_1726 YP_003646683.1 296139440 Tsukamurella paurometabola DSM 20162 CPCC7001_1320 ZP_05045132.1 254431429 Cyanobium PCC7001 DDBDRAFT_0187729 XP_636931.1 66806417 Dictyostelium discoideum AX4

An additional enzyme candidate found in Streptomyces griseus is encoded by the griC and griD genes. This enzyme is believed to convert 3-amino-4-hydroxybenzoic acid to 3-amino-4-hydroxybenzaldehyde as deletion of either griC or griD led to accumulation of extracellular 3-acetylamino-4-hydroxybenzoic acid, a shunt product of 3-amino-4-hydroxybenzoic acid metabolism (Suzuki, et al., J. Antibiot. 60(6):380-387 (2007)). Co-expression of griC and griD with SGR_665, an enzyme similar in sequence to the Nocardia iowensis npt, can be beneficial.

GenBank Gene name GI No. Accession No. Organism griC YP_001825755.1 182438036 Streptomyces griseus subsp. griseus NBRC 13350 grip YP_001825756.1 182438037 Streptomyces griseus subsp. griseus NBRC 13350

Example XIII Production of 1,3-Butanediol from Glucose in Saccharomyces cerevisiae

This example illustrates the construction and biosynthetic production of 1,3-BDO from glucose in Saccharomyces cerevisiae.

The pathway for 1,3-BDO production is comprised of two MI-FAE cycle enzymes (thiolase and 3-oxoacyl-CoA reductase), in conjunction with termination pathway enzymes (acyl-CoA reductase (aldehyde forming) and alcohol dehydrogenase). The 1,3-BDO pathway engineered into S. cerevisiae is composed of four enzymatic steps which transform acetyl-CoA to 1,3-BDO. The first step entails the condensation of two molecules of acetyl-CoA into acetoacetyl-CoA by an acetoacetyl-CoA thiolase enzyme (THL). In the second step, acetoacetyl-CoA is reduced to 3-hydroxybutyryl-CoA by acetoacetyl-CoA reductase, also called 3-hydroxybutyryl-CoA dehydrogenase (HBD). 3-hydroxybutyryl-CoA reductase (ALD) catalyzes formation of the aldehyde from the acyl-CoA. Further reduction of 3-hydroxybutyraldehyde to 1,3-BDO is catalyzed by 1,3-BDO dehydrogenase (ADH).

To enable 13-BDO production in the cytosol, two acetyl-CoA forming pathways were engineered into S. cerevisiae. The first pathway entails conversion of pyruvate to acetyl-CoA by pyruvate decarboxylase (FIG. 3E), acetaldehyde dehydrogenase (FIG. 3F) and acetyl-CoA synthetase (FIG. 3B). The second pathway is pyruvate formate lyase (FIG. 3H).

For each enzymatic step of the 1,3-BDO pathway, a list of applicable genes was assembled for corroboration. The genes cloned and assessed in this study are presented below in Table 1, along with the appropriate references and URL citations to the polypeptide sequence.

TABLE 1 Acetoacetyl-CoA thiolase (THL) Exemplary Step ID Gene NCBI Accession # GI Source Organism FIG. 2A 1502 thiI P45359.1 1174677 Clostridium acetobutylicum ATCC 824 FIG. 2A 1491 atoB NP_416728 16130161 Escherichia coli str. K-12 substr. MG1655 FIG. 2A 560 thiA NP_349476.1 15896127 Clostridium acetobutylicum ATCC 824 FIG. 2A 1512 phbA P07097.4 135759 Zoogloea ramigera FIG. 2A 1501 phbA P14611.1 135754 Ralstonia eutropha H16 3-Hydroxybutyryl-CoA dehydrogenase (HBD) FIG. 2B 1495 hbd AAM14586.1 20162442 Clostridium beijerinckii NCIMB 8052 3-Hydroxybutryl-CoA reductase (ALD) FIG. 2E 707 Lvis_1603 YP_795711.1 116334184 Lactobacillus brevis ATCC 367 3-Hydroxybutyraldehyde reductase (ADH) FIG. 2F 28 bdh BAF45463.1 124221917 Clostridium saccharoperbutylacetonicum Pyruvate formate lyase (PflAB) FIG. 3H 1799 pflA NP_415422.1 16128869 Escherichia coli MG1655 FIG. 3H 500 pflB NP_415423 16128870 Escherichia coli MG1655 PDH Bypass (aldehyde dehydrogenase, acetyl-CoA synthase) FIG. 3F 1849 ALD6 NP_015264.1 6325196 Saccharomyces cerevisiae S288c FIG. 3B 1845 Acs AAL23099.1 16422835 Salmonella enterica LT2 FIG. 3B 1845A Acsm AAL23099.1 16422835 Salmonella enterica LT2

Genes were cloned via PCR from the genomic DNA of the native or wild-type organism. Primers used to amplify the pathway genes are (from 5′ to 3′; underlined sequences are gene specific):

TM 1502: (SEQ ID NO: 8) FP: TCTAATCTAAGTTTTCTAGAACTAGTAAAGATGAGAGATGTAGTAATAGTAAGT GCTGTA  (SEQ ID NO: 9) RP: GATATCGAATTCCTGCAGCCCGGGGGATCCTTAGTCTCTTTCAACTACGAGAGC TGTT TM 1491: (SEQ ID NO: 10) FP: TCTAATCTAAGTTTTCTAGAACTAGTAAAGATGAAAAATTGTGTCATCGTCAGT G (SEQ ID NO: 11) RP: GATATCGAATTCCTGCAGCCCGGGGGATCCTTAATTCAACCGTTCAATCACCAT CGCAAT  TM 560: (SEQ ID NO: 12) FP: AATCTAAGTTTTCTAGAACTAGTAAAGATGAAAGAAGTTGTAATAGCTAGTGCA GTAA (SEQ ID NO: 13) RP: TATCGAATTCCTGCAGCCCGGGGGATCCTTAATGGTGATGGTGATGATGGCACT TTTCTA TM 1512: (SEQ ID NO: 14) FP: TCTAATCTAAGTTTTCTAGAACTAGTAAAGATGAGCACCCCGTCCATCGTCA (SEQ ID NO: 15) PR: GATATCGAATTCCTGCAGCCCGGGGGATCCCTAAAGGCTCTCGATGCACATCGC C  TM 1501: (SEQ ID NO: 16) FP: TAAGCTAGCAAGAGGAGAAGTCGACATGACTGACGTTGTCATCGTATCCGC (SEQ ID NO: 17) RP: GCCTCTAGGAAGCTTTCTAGATTATTATTTGCGCTCGACTGCCAGC Hbd 1495: (SEQ ID NO: 18) FP: AAGCATACAATCAACTATCTCATATACAATGAAAAAGATTTTTGTACTTGGAGC A  (SEQ ID NO: 19) RP: AAAAATCATAAATCATAAGAAATTCGCTTATTTAGAGTAATCATAGAATCCTTT TCCTGA Aid 707: (SEQ ID NO: 20) FP: AATCTAAGTTTTCTAGAACTAGTAAAGATGAACACAGAAAACATTGAACAAGC CAT (SEQ ID NO: 21) RP: TATCGAATTCCTGCAGCCCGGGGGATCCCTAAGCCTCCCAAGTCCGTAATGAGA ACCCTT Adh 28: (SEQ ID NO: 22) FP: CCAAGCATACAATCAACTATCTCATATACAATGGAGAATTTTAGATTTAATGCA TATACA (SEQ ID NO: 23) RP: AATAAAAATCATAAATCATAAGAAATTCGCTTAAAGGGACATTTCTAAAATTTT ATATAC

1845A is a sequence variant of the wild type (1845) enzyme. The variation is a point mutation in the residue Leu-641 (L641P), described in Starai and coworkers (Starai et al, J Biol Chem 280: 26200-5 (2005)). The function of the mutation, e.g., is to prevent post-translational regulation by acetylation and maintain the Acs enzyme in its active state.

Shuttle plasmids shown in Table 2 were constructed for expression of heterologous genes in S. cerevisiae. Plasmids d9, d10, and d11 are empty plasmid controls with the selection marker of Ura, His, and Leu, respectively. Plasmids d12 or d13 contains a single ALD or ADH gene with the URA3 selection marker. Plasmids d14, d16, and d17 contains hbd and thil genes with the HIS3 selection marker.

TABLE 2 Plasmid Selection Marker Gene(s) pESC-L URA3 NA pESC-H HIS3 NA pESC-U LEU2 NA pY3Hd1 URA3 1799(pflA)-500(pflB) pY3Hd2 HIS3 1799(pflA)-500(pflB) pY3Hd3 LEU2 1799(pflA)-500(pflB) pY3Hd4 URA3 1849(ALD6)-1845(Acs) pY3Hd5 URA3 1849(ALD6)-1845A(Acsm) pY3Hd6 URA3 1495(Hbd)-1491(Thl) pY3Hd7 URA3 1495(Hbd)-560(Thl) pY3Hd8 LEU2 28(ADH)-707(ALD) pY3Hd9 URA3 NA pY3Hd10 HIS3 NA pY3Hd11 LEU2 NA pY3Hd12 URA3 707(ALD) pY3Hd13 URA3 28(ADH) pY3Hd14 HIS3 1495(Hbd)-1502(Thl) pY3Hd15 HIS3 1495(Hbd)-1512(Thl) pY3Hd16 HIS3 1495(Hbd)-1491(Thl) pY3Hd17 HIS3 1495(Hbd)-560 (Thl)

Yeast host BY4741 [MΔTa his3Δ0 leu2Δ0 met 15Δ0 ura3Δ0] was chosen as the host strain for this work as a wild-type laboratory strain with the appropriate auxotrophic markers to host the pathway plasmids. BY4741 was transformed with plasmids containing 1,3-BDO pathway genes alone or along with plasmids that contain PDH bypass genes or pflAB genes. Vector backbones used in this example include p427TEF yeast expression vectors, the pY3H bridging vectors (Sunrise Science) and pESC yeast epitope tagging vectors (Agilent Technologies). The pY3H vector containing a TEF1 promoter, CYC terminator and URA3 selection marker from S. cerevisiae was used to build dual-promoter plasmids with different selection markers. ADH1 promoter and terminator sequences from S. cerevisiae were inserted upstream of the TEF1 promoter so the two transcriptional units are in a back-to-back orientation. The SV40 nuclear localization signal sequence was removed during the cloning process. The resulting plasmid was named pY3Hd9. To construct plasmids with a different selection marker, the URA3 gene in pY3Hd9 was replaced with the HIS3 or LEU2 gene from S. cerevisiae to produce pY3Hd10 and pY3Hd11, respectively. Two of the four 1,3-BDO pathway genes—Hbd and Thl (see Table 103 for gene numbers)—were cloned into the dual-promoter plasmid with the HIS3 marker such that the expression of the Hbd genes is controlled by the ADH1 promoter while the expression of the Thl gene is controlled by the TEF1 promoter (pY3Hd14˜17). Ald and Adh genes were cloned into the dual-promoter plasmid with the LEU2 selection marker such that the ADH1 promoter drives the adh genes and the TEF1 promoter drives the ald genes (pY3Hd8). The PflAB genes or the PDH bypass genes (ALD6 and acs) were cloned into the dual-promoter plasmid with the URA3 marker where pflA or ALD6 is controlled under the ADH1 promoter and pflB or acs is controlled under the TEF1 promoter. Yeast transformation was done using Frozen-EZ Yeast Transformation (Zymo Research).

Tables 3 and 4 show the combinations of plasmids and experimental conditions tested.

TABLE 3 Sample Plasmid 1 Plasmid 2 plasmid 3 gene 1 gene 2 gene 3 gene 4 gene 5 gene 6 Aeroation Note 1 pESC-L pESC-H Anaerobic EV2 2 pESC-L pESC-H 23G EV2 3 d8 d16 1495 1491 28 707 Anaerobic BDO 4 d8 d16 1495 1491 28 707 Anaerobic BDO 5 d8 d16 1495 1491 28 707 23G BDO 6 d8 d16 1495 1451 28 707 23G BDO 7 d8 d17 1495 560 28 707 Anaerobic BDO 8 d8 d17 1495 560 28 707 Anaerobic BDO 9 d8 d17 1495 560 28 707 23G BDO 10 d8 d17 1495 560 28 707 23G BDO 11 pESC-H pESC-L pESC-U Anaerobic EV3 12 pESC-H pESC-L pESC-U 23G EV3 13 d8 d16 d1 1495 1491 28 707 pflA pflB Anaerobic BDO + pflAB 14 d8 d16 d1 1495 1491 28 707 pflA pflB Anaerobic BDO + pflAB 15 d8 d16 d1 1495 1491 28 707 pflA pflB 23G BDO + pflAB 16 d8 d16 d1 1495 1491 28 707 pflA pflB 23G BDO + pflAB 17 d8 d17 d1 1495 560 28 707 pflA pflB Anaerobic BDO + pflAB 18 d8 d17 d1 1495 560 28 707 pflA pflB Anaerobic BDO + pflAB 19 d8 d17 d1 1495 560 28 707 pflA pflB 23G BDO + pflAB 20 d8 d17 d1 1495 560 28 707 pflA pflB 23G BDO + pflAB 21 d8 d16 d5 1495 1491 28 707 ALD6 acsm Anaerobic BDO + PDH 22 d8 d16 d5 1495 1491 28 707 ALD6 acsm Anaerobic BDO + PDH 23 d8 d16 d5 1495 1491 28 707 ALD6 acsm 23G BDO + PDH 24 d8 d16 d5 1495 1491 28 707 ALD6 acsm 23G BDO + PDH 25 d8 d17 d5 1495 560 28 707 ALD6 acsm Anaerobic BDO + PDH 26 d8 d17 d5 1495 560 28 707 ALD6 acsm Anaerobic BDO + PDH 27 d8 d17 d5 1495 560 28 707 ALD6 acsm 23G BDO + PDH 28 d8 d17 d5 1495 560 28 707 ALD6 acsm 23G BDO + PDH

TABLE 4 Plasmid 1 Plasmid 2 plasmid 3 gene 1 gene 2 gene 3 gene 4 gene 5 gene 6 Aeroation Note d9 d11 aerobic EVC d8 d17 1495 560 28 707 aerobic BDO d8 d17 d5 1495 560 28 707 1849 1845A aerobic BDO + PDH d8 d14 1495 1502 28 707 aerobic BDO d8 d14 d5 1495 1502 28 707 1849 1845A aerobic BDO + PDH

In Table 3, colonies were inoculated in 5 ml of 2% glucose medium with corresponding amino acid dropouts and cultured at 30 degree for approximately 48 Ins. Cells were briefly spun down and re-suspended in 2 ml fresh 2% glucose medium with tween-80 and ergosterol added. Resuspended cultures were added to 10 ml fresh glucose medium in 20 ml bottles to obtain a starting OD of 0.2. For anaerobic cultures, the bottles containing cultures were vacuumed and filled with nitrogen. For micro-aerobic growth, a 23G needle was inserted. All the cultures were incubated at 30 degree with shaking for 24 hours. In Table 4, the experiment was carried out in a 96-well plate and cells grown aerobically in 1.2 ml of medium with varying glucose and acetate concentrations (5% glucose, 10% glucose, 5% glucose+50 mM acetate, and 10% glucose+50 mM acetate).

Concentrations of glucose, 1,3-BDO, alcohols, and other organic acid byproducts in the culture supernatant were determined by HPLC using an HPX-87H column (BioRad).

MI-FAE cycle and termination pathway genes were tested with or without pflAB or PDH bypass. As shown in FIGS. 9-11, these constructs produced 0.3-3.35 mM 1,3-BDO in yeast S. cerevisiae BY4741, and ethanol was produced in the tested samples tested. The PDH bypass (here, overexpression of ALD6 and acs or acsm genes) improved production of 1,3-BDO.

Example XIV Enzymatic Activity of 1,3-Butanediol Pathway Enzymes

This example describes the detection of 1,3-BDO pathway enzyme activity using in vitro assays.

Activity of the heterologous enzymes was tested in in vitro assays, using an internal yeast strain as the host for the plasmid constructs containing the pathway genes. Cells were grown aerobically in yeast media containing the appropriate amino acid for each construct. To obtain elude extracts for activity assays, cells were harvested by centrifugation. The pellets were resuspended in 0.1 mL 100 mM Tris pH 7.0 buffer containing protease inhibitor cocktail. Lysates were prepared using the method of bead beating for 3 min. Following bead beating, the solution was centrifuged at 14,000 rpm (Eppendorf centrifuge 5402) for 15 min at 4° C. Cell protein in the sample was determined using the method of Bradford et al., Anal. Biochem. 72:248-254 (1976), and specific enzyme assays conducted as described below.

Thiolase

Thiolase enzymes catalyze the condensation of two acetyl-CoA to form acetoacetyl-CoA. In the reaction, coenzyme A (CoA) is released and the free CoA can be detected using 5,5′-dithiobis-2-nitrobenzoic acid (DTNB) which absorbs at 410 nm upon reaction with CoA. Five thiolases were tested (see Example XIII, Table 1). Estimated specific activity in E. coli crude lysates is shown in FIG. 13.

Among the Thl that showed expressed protein, 1512 and 1502 demonstrated the highest specific activity for acetyl-CoA condensation activity n E. coli crude lysates.

Both 1491 and 560 were cloned in dual promoter yeast vectors with 1495, which is the 3-hydroxybutyryl-CoA dehydrogenase (see FIG. 14). These thiolases were evaluated for acetyl-CoA condensation activity, and the data is shown in FIG. 14. The results indicate that both 560 and 1491 demonstrate an initial burst of activity that is too fast to measure. However, after the initial enzyme rate, the condensation rate of 560 is greater than 1491. Thus, there is protein expression and active enzyme with the yeast dual promoter vectors as indicated by active thiolase activity observed in crude lysates.

3-Hydroxybutyryl-CoA Dehydrogenase (Hbd)

Acetoacetyl-CoA is metabolized to 3-hydroxybutyryl-CoA by 3-hydroxybutyryl-CoA dehydrogenase. The reaction requires oxidation of NADH, which can be monitored by fluorescence at an excitation wavelength at 340 nm and an emission at 460 nm. The oxidized form, NAD+, does not fluoresce. This detection strategy was used for all of the dehydrogenase steps. 1495, the Hbd from Clostridium beijerinckii, was assayed in the dual promoter yeast vectors that contained either 1491 (vector id=pY3Hd17) or 560 (vector id=pY3Hd16). See Table 1 for GenBank identifiers of each enzyme. The time course data is shown in FIG. 15.

The Hbd rate of 1495 containing 560 was much faster than 1491. The results provided in FIG. 16 show that the Hbd prefers NADH over NADPH. The Hbd enzyme appears to display the fastest catalytic activity among the four pathway enzymes in crude lysates. The Hbd enzyme, i.e. a 3-ketoacyl-CoA reductase, is an example of a MI-FAE cycle or MD-FAE cycle enzyme that preferentially reacts with an NADH cofactor.

Aldehyde Deyhdrogenase (Ald)

An aldehyde reductase converts 3-hydroxybutyryl-CoA to 3-hydroxybutyraldehyde. This reaction requires NAD(P)H oxidation, which can be used to monitor enzyme activity. The Aid from Lactobacillus brevis (Gene ID 707) was cloned in a dual vector that contained the alcohol dehydrogenase from Clostridium saccharoperbutylacetonicum (Gene ID 28). These two enzymes were cloned in another dual promoter yeast vector containing a Leu marker.

The Ald activity data for crude lysates is shown in FIG. 17 with a 707 lysate from E. coli used as a standard. The results indicate the 707 showed enzyme activity in yeast lysates that is comparable to the lysate from bacteria. In addition, the 707 gene product prefers NADH to NADPH as the cofactor. The 707 gene product, i.e. an acy-CoA reductase (aldehyde forming), is an example of a termination pathway enzyme that preferentially reacts with an NADH cofactor.

Alcohol Dehydrogenase (Adh)

1,3-BDO is formed by an alcohol dehydrogenase (Adh), which reduces 3-hydroxybutyraldehyde in the presence of NAD(P)H. The oxidation of NAD(P)H can be used to monitor the reaction as described above.

The evaluation of ADH (Gene 28) in the dual promoter vector with ALD (Gene 707) is shown in FIG. 18 with butyraldehyde, a surrogate substrate for 3-hydroxybutyraldehyde. The data indicate that Gene 28 have Adh activity similar to the no insert control (EV) with butyraldehyde and NADPH. This is likely caused by endogenous ADH enzymes present in yeast that may function in the same capability as 28.

In summary, candidates for the Thl, Hbd, Aid, and Adh to produce 1,3-BDO showed enzyme activity in yeast crude lysates for the dual promoter vectors constructed.

Example XV Isopropanol Synthesis Pathway

This example describes enzymes for converting acetyl-CoA to isopropanol. Pathways are shown in FIG. 11. Enzymes for catalyzing steps T-Y are disclosed herein.

Isopropanol production was achieved in recombinant E. coli following expression of two heterologous genes from C. acetobutylicum (thl and adc encoding acetoacetyl-CoA thiolase and acetoacetate decarboxylase, respectively) and one from C. beijerinckii (adh encoding a secondary alcohol dehydrogenase), along with the increased expression of the native atoA and atoD genes which encode acetoacetyl-CoA:acetate:CoA transferase activity (Hanai et al., Appl. Environ. Microbiol. 73:7814-7818 (2007)). The acetoacetyl-CoA thiolase (AtoB) enzymes are described herein.

Acetyl-CoA:Acetyl-CoA Acyltransferase (Acetoacetyl-CoA Thiolase)—Step V, FIG. 11

Acetoacetyl-CoA thiolase (also known as acetyl-CoA acetyltransferase) converts two molecules of acetyl-CoA into one molecule each of acetoacetyl-CoA and CoA. Exemplary acetoacetyl-CoA thiolase enzymes include the gene products of atoB from E. coli (Martin et al., Nat. Biotechnol 21:796-802 (2003)), thlA and thlB from C. acetobutylicum (Hanai et al., Appl Environ Microbiol 73:7814-7818 (2007); Winzer et al., J. Mol. Microbiol Biotechnol 2:531-541 (2000), and ERG10 from S. cerevisiae Hiser et al., J. Biol. Chem. 269:31383-31389 (1994)). These genes/proteins are identified in the Table below.

Gene GenBank ID GI Number Organism AtoB NP_416728 16130161 Escherichia coli ThlA NP_349476.1 15896127 Clostridium acetobutylicum ThlB NP_149242.1 15004782 Clostridium acetobutylicum ERG10 NP_015297 6325229 Saccharomyces cerevisiae

Acetyl-CoA carboxylase (6.4.1.2)—Step T, FIG. 11

The conversion of acetyl-CoA to malonyl-CoA can be carried out by acetyl-CoA carboxylase. The E. coli enzyme complex is composed of two catalytic units and one carrier protein, encoded by four different genes. The catalytic units are biotin carboxylase (6.3.4.14), a homodimer encoded by the accC gene, and acetyl-CoA carboxylase (ACCT), an α₂β₂ tetramer, encoded by the accA and accD genes. The carrier protein is the biotin carboxyl carrier protein, a homodimer encoded by accB. Several such candidates can be found in US20120142979.

Accession GI Gene number Number Organism accA AAC73296.1 1786382 Escherichia coli K-12 accB AAC76287.1 1789653 Escherichia coli K-12 accC AAC76288.1 1789654 Escherichia coli K-12 accD AAC75376.1 1788655 Escherichia coli K-12 accA CAD08690.1 16501513 Salmonella enterica accB CAD07894.1 16504441 Salmonella enterica accC CAD07895.1 16504442 Salmonella enterica accD CAD07598.1 16503590 Salmonella enterica YMR207C NP_013934.1 6323863 Saccharomyces cerevisiae YNR016C NP_014413.1 6324343 Saccharomyces cerevisiae YGR037C NP_011551.1 6321474 Saccharomyces cerevisiae YKL182W NP_012739.1 6322666 Saccharomyces cerevisiae YPL231W NP_015093.1 6325025 Saccharomyces cerevisiae accA ZP_00618306.1 69288468 Kineococcus radiotolerans accB ZP_00618387.1 69288621 Kineococcus radiotolerans accC ZP_00618040.1/ 69287824/ Kineococcus radiotolerans ZP_00618387.1 69288621 accD ZP_00618306.1 69288468 Kineococcus radiotolerans

Acetoacetyl-CoA synthase (EC 2.3.1.194)—Step U, FIG. 11

Acetoacetyl-CoA can also be synthesized from acetyl-CoA and malonyl-CoA by acetoacetyl-CoA synthase (EC 2.3.1.194). This enzyme (FhsA) has been characterized in the soil bacterium Streptomyces sp. CL190 where it participates in mevalonate biosynthesis (Okamura et al, PNAS USA 107:11265-70 (2010)). As this enzyme catalyzes an essentially irreversible reaction, it is particularly useful for metabolic engineering applications for overproducing metabolites, fuels or chemicals derived from acetoacetyl-CoA such as long chain alcohols. Other acetoacetyl-CoA synthase genes can be identified by sequence homology to fhsA. Acyl-CoA synthase enzymes such as fhsA and homologs can be engineered or evolved to accept longer acyl-CoA substrates by methods known in the art.

Protein GenBank ID GI Number Organism fhsA BAJ83474.1 325302227 Streptomyces sp CL190 AB183750.1: BAD86806.1 57753876 Streptomyces sp. 11991 . . . 12971 KO-3988 epzT ADQ43379.1 312190954 Streptomyces cinnamonensis ppzT CAX48662.1 238623523 Streptomyces anulatus O3I_22085 ZP_09840373.1 378817444 Nocardia brasiliensis

Acetoacetyl-CoA Transferase—Step W, FIG. 11

The conversion of acetoacetyl-CoA to acetoacetate can be carried out by an acetoacetyl-CoA transferase.

These enzymes conserve the energy stored in the CoA-ester bonds of acetoacetyl-CoA. Many transferases have broad specificity and thus may utilize CoA acceptors as diverse as acetate, succinate, propionate, butyrate, 2-methylacetoacetate, 3-ketohexanoate, 3-ketopentanoate, valerate, crotonate, 3-mercaptopropionate, propionate, vinylacetate, butyrate, among others. Acetoacetyl-CoA transferase catalyzes the conversion of acetoacetyl-CoA to acetoacetate while transferring the CoA moiety to a CoA acceptor molecule. Several exemplary transferase enzymes capable of catalyzing this transformation are provided below. These enzymes either naturally exhibit the desired acetoacetyl-CoA transferase activity or they can be engineered via directed evolution to accept acetoacetyl-CoA as a substrate with increased efficiency.

In one embodiment an exemplary acetoacetyl-CoA transferase is acetoacetyl-CoA:acetate-CoA transferase. This enzyme naturally converts acetate to acetyl-CoA while converting acetoacetyl-CoA to acetoacetate. In another embodiment, a succinyl-CoA:3-ketoacid CoA transferase (SCOT) catalyzes the conversion of the 3-ketoacyl-CoA, acetoacetyl-CoA, to the 3-ketoacid, acetoacetate.

Acetoacetyl-CoA:acetyl-CoA transferase naturally converts acetoacetyl-CoA and acetate to acetoacetate and acetyl-CoA. This enzyme can also accept 3-hydroxybutyryl-CoA as a substrate or could be engineered to do so. Exemplary enzymes include the gene products of atoAD from E. coli (Hanai et al., Appl Environ Microbiol 73:7814-7818 (2007)), ctfAB from C. acetobutylicum (Jojima et al., Appl Microbiol Biotechnol 77:1219-1224 (2008)), and ctfAB from Clostridium saccharoperbutylacetonicum (Kosaka et al., Biosci. Biotechnol Biochem. 71:58-68 (2007)). Information related to these proteins and genes is shown below:

Protein GENBANK ID GI NUMBER ORGANISM AtoA P76459.1 2492994 Escherichia coli AtoD P76458.1 2492990 Escherichia coli CtfA NP_149326.1 15004866 Clostridium acetobutylicum CtfB NP_149327.1 15004867 Clostridium acetobutylicum CtfA AAP42564.1 31075384 Clostridium saccharoperbutylacetonicum CtfB AAP42565.1 31075385 Clostridium saccharoperbutylacetonicum

Succinyl-CoA:3-ketoacid-CoA transferase naturally converts succinate to succinyl-CoA while converting a 3-ketoacyl-CoA to a 3-ketoacid. Exemplary succinyl-CoA:3:ketoacid-CoA transferases are present in Helicobacter pylori (Corthesy-Theulaz et al., J. Biol. Chem. 272:25659-25667 (1997)), Bacillus subtilis (Stols et al., Protein. Expr. Purif. 53:396-403 (2007)), and Homo sapiens (Fukao et al., Genomics 68:144-151 (2000); Tanaka et al., Mol. Hum. Reprod. 8:16-23 (2002)). Information related to these proteins and genes is shown below:

Protein GENBANK ID GI NUMBER ORGANISM HPAG1_0676 YP_627417 108563101 Helicobacter pylori HPAG1_0677 YP_627418 108563102 Helicobacter pylori ScoA NP_391778 16080950 Bacillus subtilis ScoB NP_391777 16080949 Bacillus subtilis OXCT1 NP_000427 4557817 Homo sapiens OXCT2 NP_071403 11545841 Homo sapiens

Additional suitable acetoacetyl-CoA transferases are encoded by the gene products of cat1, cat2, and cat3 of Clostridium kluyveri. These enzymes have been shown to exhibit succinyl-CoA, 4-hydroxybutyryl-CoA, and butyryl-CoA transferase activity, respectively (Seedorf et al., Proc. Natl. Acad. Sci. USA 105:2128-2133 (2008); Sohling and Gottschalk, J Bacteriol 178:871-880 (1996)) Similar CoA transferase activities are also present in Trichomonas vaginalis (van Grinsven et al., J. Biol. Chem. 283:1411-1418 (2008)) and Trypanosoma brucei (Riviere et al., J. Biol. Chem. 279:45337-45346 (2004)). Yet another transferase capable of the desired conversions is butyryl-CoA:acetoacetate CoA-transferase. Exemplary enzymes can be found in Fusobacterium nucleatum (Barker et al., J. Bacteriol. 152(1):201-7 (1982)), Clostridium SB4 (Barker et al., J. Biol. Chem. 253(4):1219-25 (1978)), and Clostridium acetobutylicum (Wiesenbom et al., Appl. Environ. Microbiol. 55(2):323-9 (1989)). Although specific gene sequences were not provided for butyryl-CoA:acetoacetate CoA-transferase in these references, the genes FN0272 and FN0273 have been annotated as a butyrate-acetoacetate CoA-transferase (Kapatral et al., J. Bact. 184(7) 2005-2018 (2002)). Homologs in Fusobacterium nucleatum such as FN1857 and FN1856 also likely have the desired acetoacetyl-CoA transferase activity. FN1857 and FN1856 are located adjacent to many other genes involved in lysine fermentation and are thus very likely to encode an acetoacetate:butyrate CoA transferase (Kreimeyer, et al., J. Biol. Chem. 282 (10) 7191-7197 (2007)). Additional candidates from Porphyrmonas gingivalis and Thermoanaerobacter tengcongensis can be identified in a similar fashion (Kreimeyer, et al., J. Biol. Chem. 282 (10) 7191-7197 (2007)). Information related to these proteins and genes is shown below:

Protein GENBANK ID GI NUMBER ORGANISM Cat1 P38946.1 729048 Clostridium kluyveri Cat2 P38942.2 1705614 Clostridium kluyveri Cat3 EDK35586.1 146349050 Clostridium kluyveri TVAG_395550 XP_001330176 123975034 Trichomonas vaginalis G3 Tb11.02.0290 XP_828352 71754875 Trypanosoma brucei FN0272 NP_603179.1 19703617 Fusobacterium nucleatum FN0273 NP_603180.1 19703618 Fusobacterium nucleatum FN1857 NP_602657.1 19705162 Fusobacterium nucleatum FN1856 NP_602656.1 19705161 Fusobacterium nucleatum PG1066 NP_905281.1 34540802 Porphyromonas gingivalis W83 PG1075 NP_905290.1 34540811 Porphyromonas gingivalis W83 TTE0720 NP_622378.1 20807207 Thermoanaerobacter tengcongensis MB4 TTE0721 NP_622379.1 20807208 Thermoanaerobacter tengcongensis MB4

Acetoacetyl-CoA can be hydrolyzed to acetoacetate by acetoacetyl-CoA hydrolase. Many CoA hydrolases (EC 3.1.2.1) have broad substrate specificity and are suitable enzymes for these transformations either naturally or following enzyme engineering. Though the sequences were not reported, several acetoacetyl-CoA hydrolases were identified in the cytosol and mitochondrion of the rat liver (Aragon and Lowenstein, J. Biol. Chem. 258(8):4725-4733 (1983)). Additionally, an enzyme from Rattus norvegicus brain (Robinson et al., Biochem. Biophys. Res. Commun. 71:959-965 (1976)) can react with butyryl-CoA, hexanoyl-CoA and malonyl-CoA. The acot12 enzyme from the rat liver was shown to hydrolyze C2 to C6 acyl-CoA molecules (Suematsu et al., Eur. J. Biochem. 268:2700-2709 (2001)). Though its sequence has not been reported, the enzyme from the mitochondrion of the pea leaf showed activity on acetyl-CoA, propionyl-CoA, butyryl-CoA, palmitoyl-CoA, oleoyl-CoA, succinyl-CoA, and crotonyl-CoA (Zeiher and Randall, Plant. Physiol. 94:20-27 (1990)). Additionally, a glutaconate CoA-transferase from Acidaminococcus fermentans was transformed by site-directed mutagenesis into an acyl-CoA hydrolase with activity on glutaryl-CoA, acetyl-CoA and 3-butenoyl-CoA (Mack and Bucket, FEBS Lett. 405:209-212 (1997)). This indicates that the enzymes encoding acetoacetyl-CoA transferases can also be used as hydrolases with certain mutations to change their function. The acetyl-CoA hydrolase, ACH1, from S. cerevisiae represents another candidate hydrolase (Buu et al., J. Biol. Chem. 278:17203-17209 (2003)). Information related to these proteins and genes is shown below:

Protein GENBANK ID GI NUMBER ORGANISM Acot12 NP_570103.1 18543355 Rattus norvegicus GctA CAA57199 559392 Acidaminococcus fermentans GctB CAA57200 559393 Acidaminococcus fermentans ACH1 NP_009538 6319456 Saccharomyces cerevisiae

Another candidate hydrolase is the human dicarboxylic acid thioesterase, acot8, which exhibits activity on glutaryl-CoA, adipyl-CoA, suberyl-CoA, sebacyl-CoA, and dodecanedioyl-CoA (Westin et al., J. Biol. Chem. 280:38125-38132 (2005)) and the closest E. coli homolog, tesB, which can also hydrolyze a broad range of CoA thioesters (Naggert et al., J. Biol. Chem. 266:11044-11050 (1991)) including 3-hydroxybutyryl-CoA (Tseng et al., Appl. Environ. Microbiol. 75(10):3137-3145 (2009)). A similar enzyme has also been characterized in the rat liver (Deana, Biochem. Int. 26:767-773 (1992)). Other potential E. coli thioester hydrolases include the gene products of tesA (Bonner and Bloch, J. Biol. Chem. 247:3123-3133 (1972)), ybgC (Kuznetsova et al., FEMS Microbiol. Rev. 29:263-279 (2005); Zhuang et al., FEBS Lett. 516:161-163 (2002)), paaI (Song et al., J. Biol. Chem. 281:11028-11038 (2006)), and ybdB (Leduc et al., J. Bacteriol. 189:7112-7126 (2007)). Information related to these proteins and genes is shown below:

Protein GENBANK ID GI NUMBER ORGANISM Acot8 CAA15502 3191970 Homo sapiens TesB NP_414986 16128437 Escherichia coli Acot8 NP_570112 51036669 Rattus norvegicus TesA NP_415027 16128478 Escherichia coli YbgC NP_415264 16128711 Escherichia coli PaaI NP_415914 16129357 Escherichia coli YbdB NP_415129 16128580 Escherichia coli

Additional hydrolase enzymes include 3-hydroxyisobutyryl-CoA hydrolase which has been described to efficiently catalyze the conversion of 3-hydroxyisobutyryl-CoA to 3-hydroxyisobutyrate during valine degradation (Shimomura et al., J. Biol. Chem. 269:14248-14253 (1994)). Genes encoding this enzyme include hibch of Rattus norvegicus (Shimomura et al., supra (1994); Shimomura et al., Methods Enzymol. 324:229-240 (2000)) and Homo sapiens (Shimomura et al., supra (1994). Candidate genes by sequence homology include hibch of Saccharomyces cerevisiae and BC_2292 of Bacillus cereus. BC_2292 was shown to demonstrate 3-hydroxybutyryl-CoA hydrolase activity and function as part of a pathway for 3-hydroxybutyrate synthesis when engineered into Escherichia coli (Lee et al., Appl. Microbiol. Biotechnol. 79:633-641 (2008)). Information related to these proteins and genes is shown below:

Protein GENBANK ID GI NUMBER ORGANISM Hibch Q5XIE6.2 146324906 Rattus norvegicus Hibch Q6NVY1.2 146324905 Homo sapiens Hibch P28817.2 2506374 Saccharomyces cerevisiae BC_2292 AP09256 29895975 Bacillus cereus ATCC 14579

The hydrolysis of acetoacetyl-CoA can alternatively be carried out by a single enzyme or enzyme complex that exhibits acetoacetyl-CoA hydrolase activity. This activity enables the net hydrolysis of the CoA-ester of either molecule, and in some cases, results in the simultaneous generation of ATP. For example, the product of the LSC1 and LSC2 genes of S. cerevisiae and the sucC and sucD genes of E. coli naturally form a succinyl-CoA synthetase complex that catalyzes the formation of succinyl-CoA from succinate with the concomitant consumption of one ATP, a reaction which is reversible in vivo (Grays et al., U.S. Pat. No. 5,958,745, filed Sep. 28, 1999). Information related to these proteins and genes is shown below:

Protein GENBANK ID GI NUMBER ORGANISM SucC NP_415256.1 16128703 Escherichia coli SucD AAC73823.1 1786949 Escherichia coli LSC1 NP_014785 6324716 Saccharomyces cerevisiae LSC2 NP_011760 6321683 Saccharomyces cerevisiae

Additional exemplary CoA-ligases include the rat dicarboxylate-CoA ligase for which the sequence is yet uncharacterized (Vamecq et al., Biochemical J. 230:683-693 (1985)), either of the two characterized phenylacetate-CoA ligases from P. chrysogenum (Lamas-Maceiras et al., Biochem. J. 395:147-155 (2005); Wang et al., Biochem Biophy Res Commun 360(2):453-458 (2007)), the phenylacetate-CoA ligase from Pseudomonas putida (Martinez-Blanco et al., J. Biol. Chem. 265:7084-7090 (1990)), and the 6-carboxyhexanoate-CoA ligase from Bacillus subtilis (Bower et. al., J. Bacteriol. 178(14):4122-4130 (1996)). Additional candidate enzymes are acetoacetyl-CoA synthetases from Mus musculus (Hasegawa et al., Biochim. Biophys. Acta 1779:414-419 (2008)) and Homo sapiens (Ohgami et al., Biochem. Pharmacol. 65:989-994 (2003)), which naturally catalyze the ATP-dependant conversion of acetoacetate into acetoacetyl-CoA. 4-Hydroxybutyryl-CoA synthetase activity has been demonstrated in Metallosphaera sedula (Berg et al., Science 318:1782-1786 (2007)). This function has been tentatively assigned to the Msed_1422 gene. Information related to these proteins and genes is shown below:

GI Protein GENBANK ID NUMBER ORGANISM Phl CAJ15517.1 77019264 Penicillium chrysogenum PhlB ABS19624.1 152002983 Penicillium chrysogenum PaaF AAC24333.2 22711873 Pseudomonas putida BioW NP_390902.2 50812281 Bacillus subtilis AACS NP_084486.1 21313520 Mus musculus AACS NP_076417.2 31982927 Homo sapiens Msed_1422 YP_001191504 146304188 Metallosphaera sedula

ADP-forming acetyl-CoA synthetase (ACD, EC 6.2.1.13) is another candidate enzyme that couples the conversion of acyl-CoA esters to their corresponding acids with the concurrent synthesis of ATP. Several enzymes with broad substrate specificities have been described in the literature. ACD I from Archaeoglobus fulgidus, encoded by AF1211, was shown to operate on a variety of linear and branched-chain substrates including acetyl-CoA, propionyl-CoA, butyryl-CoA, acetate, propionate, butyrate, isobutyrate, isovalerate, succinate, fumarate, phenylacetate, indoleacetate (Musfeldt et al., J. Bacteriol. 184:636-644 (2002)). The enzyme from Haloarcula marismortui (annotated as a succinyl-CoA synthetase) accepts propionate, butyrate, and branched-chain acids (isovalerate and isobutyrate) as substrates, and was shown to operate in the forward and reverse directions (Brasen et al., Arch. Microbiol. 182:277-287 (2004)). The ACD encoded by PAE3250 from hyperthermophilic crenarchaeon Pyrobaculum aerophilum showed the broadest substrate range of all characterized ACDs, reacting with acetyl-CoA, isobutyryl-CoA (preferred substrate) and phenylacetyl-CoA (Brasen et al., supra (2004)). The enzymes from A. fulgidus, H. marismortui and P. aerophilum have all been cloned, functionally expressed, and characterized in E. coli (Musfeldt et al., supra; Brasen et al., supra (2004)). Information related to these proteins and genes is shown below:

Protein GENBANK ID GI NUMBER ORGANISM AF1211 NP_070039.1 11498810 Archaeoglobus fulgidus DSM 4304 Scs YP_135572.1 55377722 Haloarcula marismortui ATCC 43049 PAE3250 NP_560604.1 18313937 Pyrobaculum aerophilum str. IM2

An alternative method for removing the CoA moiety from acetoacetyl-CoA is to apply a pair of enzymes such as a phosphate-transferring acyltransferase and a kinase to impart acetoacetyl-CoA synthetase activity. Exemplary names for these enzymes include phosphotransacetoacetylase/acetoacetate kinase which can remove the CoA moiety from acetoacetyl-CoA. This general activity enables the net hydrolysis of the CoA-ester of either molecule with the simultaneous generation of ATP. For example, the butyrate kinase (buk)/phosphotransbutyrylase (ptb) system from Clostridium acetobutylicum has been successfully applied to remove the CoA group from 3-hydroxybutyryl-CoA when functioning as part of a pathway for 3-hydroxybutyrate synthesis (Tseng et al., Appl. Environ. Microbiol. 75(10):3137-3145 (2009)). Specifically, the ptb gene from C. acetobutylicum encodes an enzyme that can convert an acyl-CoA into an acyl-phosphate (Walter et al. Gene 134(1): p. 107-11 (1993)); Huang et al. J Mol Microbiol Biotechnol 2(1): p. 33-38 (2000). Additional ptb genes can be found in butyrate-producing bacterium L2-50 (Louis et al. J. Bacteriol. 186:2099-2106 (2004)) and Bacillus megaterium (Vazquez et al. Curr Microbiol 42:345-349 (2001)). Additional exemplary phosphate-transferring acyltransferases include phosphotransacetylase, encoded by pta. The pta gene from E. coli encodes an enzyme that can convert acetyl-CoA into acetyl-phosphate, and vice versa (Suzuki, T. Biochim. Biophys. Acta 191:559-569 (1969)). This enzyme can also utilize propionyl-CoA instead of acetyl-CoA forming propionate in the process (Hesslinger et al. Mol. Microbiol 27:477-492 (1998)). Information related to these proteins and genes is shown below:

Protein GENBANK ID GI NUMBER ORGANISM Pta NP_416800.1 16130232 Escherichia coli Ptb NP_349676 15896327 Clostridium acetobutylicum Ptb AAR19757.1 38425288 butyrate-producing bacterium L2-50 Ptb CAC07932.1 10046659 Bacillus megaterium

Exemplary kinases include the E. coli acetate kinase, encoded by ackA (Skarstedt and Silverstein J. Biol. Chem. 251:6775-6783 (1976)), the C. acetobutylicum butyrate kinases, encoded by buk1 and buk2 ((Walter et al. Gene 134(1):107-111 (1993); Huang et al. J Mol Microbiol Biotechnol 2(1):33-38 (2000)), and the E. coli gamma-glutamyl kinase, encoded by proB (Smith et al. J. Bacteriol. 157:545-551 (1984)). These enzymes phosphorylate acetate, butyrate, and glutamate, respectively. The ackA gene product from E. coli also phosphorylates propionate (Hesslinger et al. Mol. Microbiol 27:477-492 (1998)). Information related to these proteins and genes is shown below:

Protein GENBANK ID GI NUMBER ORGANISM AckA NP_416799.1 16130231 Escherichia coli Buk1 NP_349675 15896326 Clostridium acetobutylicum Buk2 Q97II1 20137415 Clostridium acetobutylicum ProB NP_414777.1 16128228 Escherichia coli

Acetoacetate Decarboxylase—Step X, FIG. 11

Acetoacetate decarboxylase converts acetoacetate into carbon dioxide and acetone. Exemplary acetoacetate decarboxylase enzymes are encoded by the gene products of adc from C. acetobutylicum (Petersen and Bennett, Appl. Environ. Microbiol. 56:3491-3498 (1990) and adc from Clostridium saccharoperbutylacetonicum (Kosaka et al., Biosci. Biotechnol. Biochem. 71:58-68 (2007)). The enzyme from C. beijerinkii can be inferred from sequence similarity.

Protein GenBank ID GI Number Organism Adc NP_149328.1 15004868 Clostridium acetobutylicum Adc AAP42566.1 31075386 Clostridium saccharoperbutylacetonicum Adc YP_001310906.1 150018652 Clostridium beijerinckii

Acetone Reductase or Isopropanol Dehydrogenase—Step Y, FIG. 11

The final step in the isopropanol synthesis pathway involves the reduction of acetone to isopropanol. Exemplary alcohol dehydrogenase enzymes capable of this transformation include adh from C. beijerinckii (Jojima et al., Appl. Microbiol. Biotechnol. 77:1219-1224 (2008); Hanai et al., Appl. Environ. Microbiol. 73:7814-7818 (2007) and adh from Thermoanaerobacter brockii (Hanai et al., supra; Peretz et al., Anaerobe 3:259-270 (1997)). Additional characterized enzymes include alcohol dehydrogenases from Ralstonia eutropha (formerly Alcaligenes eutrophus) (Steinbuchel and Schlegel, Eur. J. Biochem. 141:555-564 (1984) and Phytomonas species (Uttaro and Opperdoes, Mol. Biochen. Parasitol. 85: 213-219 (1997)).

Protein GenBank ID GI Number Organism Adh P14941.1 113443 Thermoanaerobobacter brockii Adh AAA23199.2 60592974 Clostridium beijerinckii Adh YP_299391.1 73539024 Ralstonia eutropha iPDH AAP39869.1 31322946 Phtomonas sp.

Example XVI Production of Fatty Alcohols, Fatty Aldehydes, and Fatty Acids Via the Fatty Acyl-ACP Elongation (FAACPE) Cycle and Termination Pathways

This example describes enzymes for converting acetyl-CoA to products of interest such as fatty alcohols, fatty aldehydes, and fatty acids through the FAACPE cycle and termination pathways. Pathways are shown in FIG. 12. Enzymes for catalyzing steps A-O are disclosed herein.

Fatty acid biosynthesis requires several steps. The initiation of fatty acid biosynthesis requires the conversion of acetyl-CoA to malonyl CoA by an enzyme called acetyl CoA carboxylase (Step A, FIG. 12). Malonyl-CoA is then converted into malonyl-ACP by a CoA-ACP transacylase (Step B, FIG. 12). This is the substrate for the first step of the elongation cycle, namely the condensation step (Steps C and H, FIG. 12). This reaction is called β-keto acyl ACP synthase. The keto group is then reduced to a hydroxyl group by a β-keto acyl ACP reductase (Step E, FIG. 12). The next step is a dehydration step that involves conversion of the hydroxyl group into an enoyl moiety (Step F, FIG. 12). This is catalyzed by a β-hydoxy acyl ACP dehydratase. The enoyl group is finally reduced to form an acyl-ACP by enoyl-ACP reductase (Step G, FIG. 12). At this point, the acyl-ACP can either be further elongated by the condensation reaction carried out by the β-keto acyl ACP synthase or can be converted into a fatty acid by a thioesterase (Step I, FIG. 12). The acid can be further activated to acyl-CoA by an acyl-CoA synthetase or ligase (Step K, FIG. 12) or can be directly reduced to an acid by a carboxylic acid reductase (CAR) (Step O, FIG. 12). The acyl-CoA can have multiple fates too and can either be converted into an aldehyde by acyl-CoA reductase (Step L, FIG. 12) or can be converted into a fatty alcohol by a fatty alcohol forming acyl-CoA reductase (FAR) (Step N, FIG. 12). The fatty aldehyde can also be converted into a fatty alcohol by a fatty aldehyde reductase (Step M, FIG. 12).

There are two basic types of fatty acid (FAS) biosynthesis mechanisms. The type I system is found in mammals and lower eukaryotes. The mammalian system consists of a single gene product that contains all of the reaction centers required to produce a fatty acid, e.g., the fatty acid synthase from Homo sapiens. In lower eukaryotes such as yeast, fatty acid synthase function is catalyzed by two genes (FAS I and FAS II), whose polypeptides form a eukaryotic complex.

Type II systems are found in bacteria and plants (White et al. (2005), The structural biology of type II fatty acid biosynthesis, Annu Rev Biochem, 74 (791-831)) among other organisms. The reactions in these systems are catalyzed by a series of individual soluble proteins that are each encoded by a discrete gene, and the pathway intermediates are transferred between the enzymes as thioesters of a holo acyl carrier protein (ACP).

Acetyl-CoA Carboxylase (6.4.1.2)—Step A, FIG. 12

The conversion of acetyl-CoA to malonyl-CoA can be carried out by acetyl-CoA carboxylase. The E. coli enzyme complex is composed of two catalytic units and one carrier protein, encoded by four different genes. The catalytic units are biotin carboxylase (6.3.4.14), a homodimer encoded by the accC gene, and acetyl-CoA carboxylase (ACCT), an α₂β₂ tetramer, encoded by the accA and accD genes. The carrier protein is the biotin carboxyl carrier protein, a homodimer encoded by accB. Several such candidates can be found in US20120142979.

Accession GI Gene number Number Organism accA AAC73296.1 1786382 Escherichia coli K-12 accB AAC76287.1 1789653 Escherichia coli K-12 accC AAC76288.1 1789654 Escherichia coli K-12 accD AAC75376.1 1788655 Escherichia coli K-12 accA CAD08690.1 16501513 Salmonella enterica accB CAD07894.1 16504441 Salmonella enterica accC CAD07895.1 16504442 Salmonella enterica accD CAD07598.1 16503590 Salmonella enterica YMR207C NP_013934.1 6323863 Saccharomyces cerevisiae YNR016C NP_014413.1 6324343 Saccharomyces cerevisiae YGR037C NP_011551.1 6321474 Saccharomyces cerevisiae YKL182W NP_012739.1 6322666 Saccharomyces cerevisiae YPL231W NP_015093.1 6325025 Saccharomyces cerevisiae accA ZP_00618306.1 69288468 Kineococcus radiotolerans accB ZP_00618387.1 69288621 Kineococcus radiotolerans accC ZP_00618040.1/ 69287824/ Kineococcus radiotolerans ZP_00618387.1 69288621 accD ZP_00618306.1 69288468 Kineococcus radiotolerans

CoA-ACP Acyltransferase (23.1.1)—Step B, FIG. 12

The exchange of an ACP moiety for a CoA is catalyzed by enzymes in EC class 2.3.1. Activation of acetyl-CoA to acetyl-ACP and malonyl-CoA to malonyl-ACP are also catalyzed by a CoA:ACP acyltransferase Enzymes with CoA-ACP acyltransferase activity include acetyl-CoA:ACP transacylase (EC 2.3.1.38) and malonyl-CoA:ACP transacylase (EC 2.3.1.39).

The FabH (KASIII) enzyme of E. coli functions as an acyl-CoA:ACP transacylase, in addition to its primary activity of forming acetoacetyl-ACP. Butyryl-ACP is accepted as an alternate substrate of FabH (Prescott et al, Adv. Enzymol. Relat. Areas Mol, 36:269-311 (1972)). Acetyl-CoA:ACP transacylase enzymes from Plasmodium falciparum and Streptomyces avermitillis have been heterologously expressed in E. coli (Lobo et al, Biochem 40:11955-64 (2001)). A synthetic KASIII (FabH) from P. falciparum expressed in a fabH-deficient Lactococcus lactis host was able to complement the native fadH activity (Du et al, AEM 76:3959-66 (2010)). The acetyl-CoA:ACP transacylase enzyme from Spinacia oleracea accepts other acyl-ACP molecules as substrates, including butyryl-ACP (Shimakata et al, Methods Enzym 122:53-9 (1986)). The sequence of this enzyme has not been determined to date. Malonyl-CoA:ACP transacylase enzymes include FabD of E. coli and Brassica napsus (Verwoert et al, J Bacteriol, 174:2851-7 (1992); Simon et al, FEBS Lett 435:204-6 (1998)). FabD of B. napsus was able to complement fabD-deficient E. coli. The multifunctional eukaryotic fatty acid synthase enzyme complexes (described in EC 2.3.1.) also catalyze this activity. More exemplary gene candidates can be found in WO2007136762A2.

Gene GenBank ID GI Number Organism fabH AAC74175.1 1787333 Escherichia coli fadA NP_824032.1 29829398 Streptomyces avermitillis fabH AAC63960.1 3746429 Plasmodium falciparum Synthetic ACX34097.1 260178848 Plasmodium falciparum construct fabH CAL98359.1 124493385 Lactococcus lactis fabD AAC74176.1 1787334 Escherichia coli fabD CAB45522.1 5139348 Brassica napsus fabD ZP_00617602.1 69286751 Kineococcus radiotolerans fabD YP_388786.1 78357337 Desulfovibrio alaskensis fabD YP_425507 83591755 Rhodospirillum rubrum Acyl-ACP C-Acyltransferase (Decarboxylating) or 13-Ketoacyl-ACP Synthase (2.3.1.e)—Steps C, D and H, FIG. 12

Acetoacetyl-ACP is formed from malonyl-ACP and either acetyl-CoA or acetyl-ACP. E. coli has three ketoacyl-ACP synthases (KAS enzymes), KAS I, KAS II and KAS III, encoded by fabB, fabF and fabH respectively. FabH (KAS III), the key enzyme of initiation of fatty acid biosynthesis in E. coli, is selective for the formation of acetoacetyl-ACP from acetyl-CoA and malonyl-ACP. Some gene candidates for this step are shown below.

Gene GenBank ID GI Number Organism fabH AAC74175.1 1787333 Escherichia coli fabH ZP_00618003.1 69287672 Kineococcus radiotolerans fabH YP_388920.1 7835747 Desulfovibrio alaskensis fabH YP_425507.1 83591755 Rhodospirillum rubrum

Alternately, acetyl-CoA can first be activated to acetyl-ACP and subsequently condensed to acetoacetyl-ACP by two enzymes, acetyl-CoA:ACP transacylase (EC 2.3.1.38) and acetoacetyl-ACP synthase (EC 2.3.1.41). Acetyl-CoA:ACP transacylase converts acetyl-CoA and an acyl carrier protein to acetyl-ACP, releasing CoA. Enzyme candidates for acetyl-CoA:ACP transacylase are described in section EC 2.3.1.f above. Acetoacetyl-ACP synthase enzymes catalyze the condensation of acetyl-ACP and malonyl-ACP. This activity is catalyzed by FabF and FabB of E. coli, as well as the multifunctional eukaryotic fatty acid synthase enzyme complexes described in EC 2.3.1.g. FabB and FabF catalyze the condensation of malonyl-ACP with acyl-ACP substrates (β-ketoacyl-ACP synthase activity) and function primarily in fatty acid elongation. Specifically, a β-ketoacyl-ACP synthase catalyzes the conversion of a saturated fatty acyl ACP and malonyl-ACP into 3-oxoacyl-ACP that is 2 carbons longer that the substrate fatty acyl ACP. When it reacts with acetyl-ACP, it participates in fatty acid initiation. The Bacillus subtilis KAS enzymes are similar to FabH but are less selective, accepting branched acyl-CoA substrates (Choi et al, J Bacteriol 182:365-70 (2000)).

Gene GenBank ID GI Number Organism fabB AAC75383.1 1788663 Escherichia coli fabF AAC74179.1 1787337 Escherichia coli FabHA NP_389015.1 16078198 Bacillus subtilis FabHB NP_388898.1 16078081 Bacillus subtilis

More exemplary gene candidates for acyl-ACP C-acyl transferase can be found in WO2007136762A2 (Production of fatty acids and derivatives thereof). Some of the enzymes listed below are from US20110250663 (Methods and compositions related to fatty alcohol biosynthetic enzymes). Several more keto-acyl synthases have been identified in these applications. Exemplary aeto Acyl-ACP synthases from E. coli are described below.

Gene GenBank ID GI number Organism fabB ACY27486.1 262176863 Escherichia coli LW1655F+ fabF ACY27487 262176865 Escherichia coli LW1655F+ fadJ ACX38989.1 260448567 Escherichia coli DH1 xerC ACX41768.1 260451346 Escherichia coli DH1 vaeF ACX38529.1 260448107 Escherichia coli DH1 murQ ACX38907.1 260448485 Escherichia coli DH1 Oxidoreductase (Oxo to Alcohol) (1.1.1.a)—Step E, FIG. 12

The reduction of 3-oxoacyl-ACP to 3-hydroxyacetyl-ACP is catalyzed by 3-oxoacyl-ACP reductase (EC 1.1.1.100). The E. coli 3-oxoacyl-ACP reductase is encoded by fabG. Key residues responsible for binding the acyl-ACP substrate to the enzyme have been elucidated (Zhang et al, J Biol Chem 278:52935-43 (2003)). Additional enzymes with this activity have been characterized in Bacillus anthracia (Zaccai et al, Prot Struct Funct Gen 70:562-7 (2008)) and Mycobacterium tuberculosis (Gurwitz, Mol Genet Genomics 282:407-16 (2009)). The beta-ketoacyl reductase (KR) domain of eukaryotic fatty acid synthase also catalyzes this activity (Smith, FASEB J, 8:1248-59 (1994)). While many FabG enzymes preferentially utilize NADH, NADH-dependent FabG enzymes also known in the art and are shown in the table below (Javidpour et al, AEM 80: 597-505 (2014)).

Gene GenBank ID GI Number Organism fabG P0AEK2.1 84028081 Escherichia coli fabG AAP27717.1 30258498 Bacillus anthracis FabG1 NP_215999.1 15608621 Mycobacterium tuberculosis FabG4 YP_003030167.1 253797166 Mycobacterium tuberculosis FabG EDM75366.1 149815845 Plesiocystis Pacifica FabG WP_018008474.1 516633699 Cupriavidus Taiwanensis FabG WP_012242413.1 501199395 Acholeplasma Laidlawii FabG EDL65432.1 148851283 Bacillus sp SG-1 Hydro-lyase (4.2.1.a)—Step F, FIG. 12

3-Hydroxyacyl-ACP dehydratase enzymes catalyze the conversion of 3-hydroacyl-ACP to trans-2-enoyl-ACP. Enzymes with this activity include FabA and FabZ of E. coli, which possess overlapping broad substrate specificities (Heath, J Biol Chem 271:1833-6 (1996)). Fatty acid synthase complexes, described above, also catalyze this reaction. The FabZ protein from Plasmodium falciparum has been crystallized (Kostrew et al, Protein Sci 14:1570-80 (2005)). Additional candidates are the mitochondrial 3-hydroxyacyl-ACP dehydratase encoded by Htd2p in yeast and TbHTD2 in Homo sapiens and Trypanosoma brucei (Kastanoitis et al, Mol Micro 53:1407-21 (2004); Kaija et al, FEBS Lett 582:729-33 (2008)).

Gene GenBank ID GI Number Organism fabA AAC74040.1 1787187 Escherichia coli fabZ AAC73291.1 1786377 Escherichia coli PfFabZ AAK83685.1 15080870 Plasmodium falciparum Htd2p NP_011934.1 6321858 Saccharomyces cerevisiae HTD2 P86397.1 281312149 Homo sapiens Enoyl ACP Reductase (13.1.a)—Step G, FIG. 12

Enoyl-ACP reductase catalyzes the formation of a saturated acyl-ACP by an NAD(P)H-dependent reduction of the enoyl-ACP double bond. The FabI protein of E. coli is a well-characterized enoyl-ACP reductase that catalyzes the reduction of enoyl substrates of length 4 to 16 carbons (Rafi et al, JBC 281:39285-93 (2006)). FabI utilizes both NADH and NADPH as a cofactor (Bergler et al, Eur J Biochem 242:689-94 (1996)) and is inhibited by acyl-ACP via product inhibition (Heath, J Biol Chem 271:1833-6 (1996)). Bacillus subtilis contains two enoyl-ACP reductase isozymes, FabI and FabL (Heath et al, J Biol Chem 275:40128-33 (2000)). The Streptococcus pneumoniae FabK protein is a triclosan-resistant flavoprotein catalyzing the same activity (Heath and Rock, Nature 406:145-6 (2000)). An additional candidate is the Pseudomonas aeruginosa FabI protein, which was recently crystallized (Lee et al, Acta Cryst Sect F 67:214-216 (2011)).

Gene GenBank ID GI Number Organism fabI P0AEK4.2 84028072 Escherichia coli fabI P54616.2 7531269 Bacillus subtilis fabL P71079.1 81817482 Bacillus subtilis fabK AAF98273.1 9789231 Streptococcus pneumoniae fabI Q9ZFE4.1 7531118 Pseudomonas aeruginosa

Fatty Acid Synthase (23.1.g), FIG. 12

Fatty acid synthase or fatty-acyl-CoA synthase are multifunctional enzyme complexes composed of multiple copies of one or more subunits and can together catalyze all the reactions required for fatty acid synthesis: activation, priming, elongation and termination (Lomakin et al, Cell 129:319-32 (2007)). The fatty acid synthase of Saccharomyces cerevisiae is a dodecamer composed of two multifunctional subunits FAS1 and FAS2. This enzyme complex catalyzes the formation of long chain fatty acids from acetyl-CoA and malonyl-CoA. The favored product of eukaryotic FAS systems is palmitic acid (C16). Similar fatty acid synthase complexes are found in Candida parapsilosis and Thermomyces lanuginosus (Nguyen et al, PLoS One 22:e8421 (2009); Jenni et al, Science 316:254-61 (2007)). The multifunctional Fas enzymes of Mycobacterium tuberculosis and mammals such as Homo sapiens are also suitable candidates (Fernandes and Kolattukudy, Gene 170:95-99 (1996) and Smith et al, Prog Lipid Res 42:289-317 (2003)).

Gene GenBank ID GI Number Organism FAS1 CAA82025.1 486321 Saccharomyces cerevisiae FAS2 CAA97948.1 1370478 Saccharomyces cerevisiae Fas1 ABO37973.1 133751597 Thermomyces lanuginosus Fas2 ABO37974.1 133751599 Thermomyces lanuginosus Fas AAB03809.1 1036835 Mycobacterium tuberculosis Fas NP_004095.4 41872631 Homo sapiens

Multiple genes are involved in fatty acid synthesis in bacteria and plants, including: 1. Acetyl-CoA: ACP transcylase (2.3.1.38)—for converting acetyl-CoA to acetyl-ACP, 2. malonyl-CoA:ACP transacylase that converts malonyl-CoA into malonyl-ACP (2.3.1.39), 3. acetyl[acp]:malonyl-[acp] C-acyl transferase (2.3.1.41) and others in fatty acid elongation. Some exemplary gene candidates for the steps are shown below.

Gene GenBank ID GI number Organism fabH AP_001717.1 89107937 Escherichia coli K12 fabB NP_416826.1 16130258 Escherichia coli K12 fabF NP_415613.1 16129058 Escherichia coli K12 fabD NP_415610.1 16129055 Escherichia coli K12 fabI NP_415804.1 16129249 Escherichia coli K12 fabA NP_415474.1 16128921 Escherichia coli K12 fabZ NP_414722.1 16128173 Escherichia coli K12 fabG NP_415611.1 16129056 Escherichia coli K12 FasII (fatty AAA34601.1 171502 Saccahromyces acid synthase, cerevisiae alpha subunit) FasI (fatty AAA34602.1 171506 Saccahromyces acid synthase, cerevisiae beta subunit) fas NP_217040.1 15609661 Mycobacterium tuberculosis H37Rv fas AAN25329.1 23326820 Bifidobacterium longum NCC2705 fas YP_003971698.1 311064972 Bifidobacterium bifidum fas AEG82252.1 334697455 Corynebacterium ulcerans Acyl Acp Thioesterase (3.1.2.a)—Step F, FIG. 12

Acyl-ACP thioesterase releases free fatty acids from Acyl-ACPs, thus terminating fatty acid biosynthesis. There are two isoforms of acyl-ACP thioesterase, FatA and FatB. Substrate specificity of these isoforms determines the chain length and level of saturated fatty acids in plants. The highest activity of FatA is with C18:1-ACP. FatA has very low activities towards other acyl-ACPs when compared with C18:1-ACP. FatB has highest activity with C16:0-ACP. It also has significant high activity with C18:1-ACP, followed by C18:0-ACP and C16:1-ACP. Kinetics studies of FatA and FatB indicate that their substrate specificities with different acyl-ACPs came from the Kcat values, rather than from Km. Km values of the two isoforms with different substrates are similar, in the micromolar order.

Exemplary enzymes include the FatA and FatB isoforms of Arabidopsis thaliana (Salas et al, Arch Biochem Biophys 403:25-34 (2002)). A number of thioesterases with different chain length specificities are listed in WO 2008/113041 and are included in the table below [seep 126 Table 2A of patent]. For example, it has been shown previously that expression of medium chain plant thioesterases like FatB from Umbellularia californica in E. coli results in accumulation of high levels of medium chain fatty acids, primarily laurate (C12:0). Similarly, expression of Cuphea palustris FatB1 thioesterase in E. coli led to accumulation of C8-10:0 acyl-ACPs (Dehesh et al, Plant Physiol 110:203-10 (1996)). Similarly, Carthamus tinctorius thioesterase, when expressed in E. coli leads to >50 fold elevation in C18:1 chain termination and release as free fatty acid (Knutzon et al, Plant Physiol 100:1751-58 (1992)). Methods for altering the substrate specificity of acyl-ACP thioesterases are also known in the art (for example, EP 1605048).

Gene GenBank ID GI number Organism fatA AEE76980.1 332643459 Arabidopsis thaliana fatA ACC41415 183176305 Mycobacterium marinum M fatA AAX54527 61741120 Helianthus annuus fatA CAC14164 10944734 Brassica juncea fatA ZP_04749108 240170449 Mycobacterium kansasii ATCC 12478 fatA ZP_04384386.1 229490548 Rhodococcus erythropolis SK121 fatA YP_885312.1 118472377 Mycobacterium smegmatis str. MC2 155 fatB AAQ08202.1 33325193 Helianthus annuus fatB AEE28300.1 332190179 Arabidopsis thaliana fatB ABI18986.1 112455672 Brassica juncea tesA NP_415027.1 16128478 Escherichia coli K12 fatB2 AAC49269.1 1292906 Cuphea hookeriana fatB1 AAC49179.1 1215718 Cuphea palustris M96568.1:94 . . . 1251 AAA33019.1 404026 Carthamus tinctorius fatB Q41635.1 8469218 Umbellularia californica tesA AAC73596.1 1786702 Escherichia coli

Several more of these candidates can be found in WO2007136762A2 (Production of fatty acids and derivatives thereof) and are described below.

Gene GenBank ID GI number Source Organism fatB1 AAA34215.1 170556 Umbellularia california fatB1 Q39513 8469217 Cuphea hookeriana fatB Q39473 8469216 Cinnamonum camphorum fatB[M141T} CAA85388 804948 Arabidopsis thaliana fatA NP 189147, 15230256; Arabidopsis thaliana NP 193041 15235555 fatA CAC39106 14148965 Brassica juncea fatA AAC72883 3859832 Cuphea hookeriana

Acyl CoA Synthetase and Acyl CoA Ligase (6.2.1.3)—Step K, FIG. 12

Fatty acids are often found in the cell in the activated form of an acyl-coA. The activation requires energy in the form of ATP. Acyl-CoAs are used in the biosynthesis of many cellular products and components, including membrane lipids. Acyl CoA cannot move across membranes. Therefore, fatty acids are transported in their free form and converted to acyl-CoAs while crossing the membrane by the enzymes acyl-CoA synthetases (ACS). These enzymes catalyze the esterification of fatty acids into the CoA thioesters concomitant with transport

Gene GenBank ID GI number Organism scs YP_135572.1 55377722 Haloarcula marismortui sucC NP_415256.1 16128703 Escherichia coli sucD AAC73823.1 1786949 Escherichia coli LSC1 NP_014785 6324716 Saccharomyces cerevisiae LSC2 NP_011760 6321683 Saccharomyces cerevisiae paaF AAC24333.2 22711873 Pseudomonas putida matB AAC83455.1 3982573 Rhizobium leguminosarum bioW NP_390902.2 50812281 Bacillus subtilis bioW CAA10043.1 3850837 Pseudomonas mendocina bioW P22822.1 115012 Bacillus sphaericus phl CAJ15517.1 77019264 Penicillium chrysogenum phlB ABS19624.1 152002983 Penicillium chrysogenum paaF AAC24333.2 22711873 Pseudomonas putida bioW NP_390902.2 50812281 Bacillus subtilis AACS NP_084486.1 21313520 Mus musculus AACS NP_076417.2 31982927 Homo sapiens acs AAC77039.1 1790505 Escherichia coli acoE AAA21945.1 141890 Ralstonia eutropha acs1 ABC87079.1 86169671 Methanothermobacter thermautotrophicus acs1 AAL23099.1 16422835 Salmonella enterica ACS1 Q01574.2 257050994 Saccharomyces cerevisiae AF1211 NP_070039.1 11498810 Archaeoglobus fulgidus AF1983 NP_070807.1 11499565 Archaeoglobus fulgidus Oxidoreductase (Acyl-ACP to Aldehyde) 1.2.1.f—Step J, FIG. 12

The reduction of an acyl-ACP to its corresponding aldehyde is catalyzed by an acyl-ACP reductase (AAR). Such a transformation is depicted in Step J of FIG. 12. Suitable enzyme candidates include the orf1594 gene product of Synechococcus elongatus PCC7942 and homologs thereof (Schirmer et al, Science, 329: 559-62 (2010)). The S. elongates PCC7942 acyl-ACP reductase is coexpressed with an aldehyde decarbonylase in an operon that appears to be conserved in a majority of cyanobacterial organisms. This enzyme, expressed in E. coli together with the aldehyde decarbonylase, conferred the ability to produce alkanes. The P. marinus AAR was also cloned into E. coli and, together with a decarbonylase, demonstrated to produce alkanes (US Application 2011/0207203).

Gene GenBank ID GI Number Organism orf1594 YP_400611.1 81300403 Synechococcus elongatus PCC7942 PMT9312_0533 YP_397030.1 78778918 Prochlorococcus marinus MIT 9312 syc0051_d YP_170761.1 56750060 Synechococcus elongatus PCC 6301 Ava_2534 YP_323044.1 75908748 Anabaena variabilis ATCC 29413 alr5284 NP_489324.1 17232776 Nostoc sp. PCC 7120 Aazo_3370 YP_003722151.1 298491974 Nostoc azollae Cyan7425_0399 YP_002481152.1 220905841 Cyanothece sp. PCC 7425 N9414_21225 ZP_01628095.1 119508943 Nodularia spumigena CCY9414 L8106_07064 ZP_01619574.1 119485189 Lyngbya sp. PCC 8106

The gene candidates for Acyl-CoA reductase (Step L), CAR (Step O), FAR (Step N) and fatty aldehyde reductase (Step M) are described elsewhere in this application.

Oxidoreductase (Acyl-ACP to Alcohol) Step O, FIG. 2 and Step P, FIG. 12

The reduction of an acyl-ACP to its corresponding alcohol is catalyzed by an acyl-ACP reductase (alcohol forming). Such a transformation is depicted in step P of FIG. 12. Fatty acyl reductase enzymes that use acyl-ACP substrates to produce alcohols are known in the art Alcohol forming acyl-ACP reductases include Maqu_2220 of Marinobacter aquaeolei VT8 and Hch_05075 of Hahella chejuensis KCTC2396 (see WO2013/048557). These enzymes convert both acyl-ACP substrates and acyl-CoA substrates to their corresponding alcohols. The M. aquaeolei AAR was previously characterized as an aldehyde reductase (Wahlen et al, AEM 75:2758-2764 (2009)) and US 2010/0203614). Alcohol forming acyl-ACP reductase enzymes are shown in the table below.

Protein GenBank ID GI Number Organism Maqu_2220 ABM19299 120324984 Marinobacter aquaeolei Hch_05075 YP_436183 83647748 Hahella chejuensis MDG893_11561 ZP_01892457.1 149374683 Marinobacter algicola DG893 HP15_810 ADP96574.1 311693701 Marinobacter adhaerens HP15 RED65_09894 ZP_01305629.1 94499091 Oceanobacter sp. RED65

Odd Chain Length Fatty Acid Biosynthesis

Fatty acids with odd numbers of carbon can be formed by a similar mechanism as shown in FIG. 12. The starting metabolite in this case is propionyl-CoA instead of acetyl-CoA. The product of malonyl-ACP and propionyl-CoA is 3-oxovaleryl-ACP. This reaction is catalyzed by a β-ketoacyl-ACP synthase (EC 2.3.1.180) as shown in FIG. 12. The subsequent steps of fatty acid biosynthesis for an odd-chain fatty acid are the same as shown in FIG. 12. Several exemplary gene candidates for this step have been listed in US20120070868 (Odd chain fatty acid derivatives) and are shown below.

Gene symbol GenBank ID GI number Organism fabH AAC74175 1787333 E. coli fabH1 NP_389015 16078198 B. subtilis 168 fabH2 NP_388898 16078081 B. subtilis 168 fabH CAB99151 9368919 Streptomyces coelicolor fabH AAA99447 870807 Streptomyces glaucescens fabH3 NP_823466 29828832 Streptomyces avermitilis MA-4680 fabH YP_002349314 217963636 Listeria monocytogenes fabH NP_645682 21282594 Staphylococcus aureus MW2 fabH AAK74580 14971886 Streptococcus pneumoniae fabH NP_722071 24380116 Streptococcus mutans UA159 fabH NP_266927 15672753 Lactococcus lactis subsp. lactis fabH YP_003687907 297626144 Propionibacterium freundenreichii subsp. Shermanii

Example XVII Production of Propionyl-CoA

This example describes enzymes for production of Propionyl-CoA. Exemplary pathways are described FIG. 22. The pathways for production of propionyl-CoA can proceed via oxaloacetate, which includes conversion of PEP into oxaloacetate either via PEP carboxykinase or PEP carboxylase. Alternatively, PEP is converted first to pyruvate by pyruvate kinase and then to oxaloacetate by methylmalonyl-CoA carboxytransferase or pyruvate carboxylase. Oxaloacetate is converted to propionyl-CoA by means of the reductive TCA cycle, a methylmutase, a decarboxylase, an epimerase and a decarboxylase.

PEP Carboxykinase

Although the net conversion of phosphoenolpyruvate to oxaloacetate is redox-neutral, the mechanism of this conversion is important to the overall energetics of the co-production pathway. The most desirable enzyme for the conversion of PEP to oxaloacetate is PEP carboxykinase which simultaneously forms an ATP while carboxylating PEP. In most organisms, however, PEP carboxykinase serves a gluconeogenic function and converts oxaloacetate to PEP at the expense of one ATP. S. cerevisiae is one such organism whose native PEP carboxykinase, PCK1, serves a gluconeogenic role (Valdes-Hevia, FEBS. Lett. 258:313-316 (1989)). E. coli is another such organism, as the role of PEP carboxykinase in producing oxaloacetate is believed to be minor when compared to PEP carboxylase, which does not form ATP, possibly due to the higher K_(m) for bicarbonate of PEP carboxykinase (Kim, Appl Environ Microbiol 70:1238-1241 (2004)). Nevertheless, activity of the native E. coli PEP carboxykinase from PEP towards oxaloacetate has been recently demonstrated in ppc mutants of E. coli K-12 (Kwon, Journal of Microbiology and Biotechnology 16:1448-1452 (2006)). These strains exhibited no growth defects and had increased succinate production at high NaHCO₃ concentrations. In some organisms, particularly rumen bacteria, PEP carboxykinase is quite efficient in producing oxaloacetate from PEP and generating ATP. Examples of PEP carboxykinase genes that have been cloned into E. coli include those from Mannheimia succiniciproducens (Lee, Biotechnol. Bioprocess Eng. 7:95-99 (2002)), Anaerobiospirillum succiniciproducens (Laivenieks, Appl Environ Microbiol 63:2273-2280 (1997)), and Actinobacillus succinogenes (Kim, Appl Environ Microbiol 70:1238-1241 (2004)). Internal experiments have also found that the PEP carboxykinase enzyme encoded by Haemophilus influenza is highly efficient at forming oxaloacetate from PEP. These proteins are identified below.

Protein GenBank ID GI Number Organism PCK1 NP_013023 6322950 Saccharomyces cerevisiae pck NP_417862.1 16131280 Escherichia coli pckA YP_089485.1 52426348 Mannheimia succiniciproducens pckA O09460.1 3122621 Anaerobiospirillum succiniciproducens pckA Q6W6X5 75440571 Actinobacillus succinogenes pckA P43923.1 1172573 Haemophilus influenza

These sequences and sequences for subsequent enzymes listed in this report can be used to identify homologue proteins in GenBank or other databases through sequence similarity searches (e.g. BLASTp). The resulting homologue proteins and their corresponding gene sequences provide additional DNA sequences for transformation into the host organism of choice.

PEP Carboxylase

PEP carboxylase represents an alternative enzyme for the formation of oxaloacetate from PEP. Since the enzyme does not generate ATP upon decarboxylating oxaloacetate, its utilization decreases the maximum ATP yield of the production pathway and represents a less favorable alternative for converting oxaloacetate to PEP. Nevertheless, the maximum theoretical C3 alcohols yield of 1.33 mol/mol will remain unchanged if PEP carboxylase is utilized to convert PEP to oxaloacetate. S. cerevisiae does not naturally encode a PEP carboxylase, but exemplary organisms that possess genes that encode PEP carboxylase include E. coli (Kai, Arch. Biochem. Biophys. 414:170-179 (2003)), Methylobacterium extorquens AM1 (Arps, J. Bacteriol. 175:3776-3783 (1993)), and Corynebacterium glutamicum (Eikmanns, Mol. Gen. Genet. 218:330-339 (1989)). These proteins are identified below.

Protein GenBank ID GI Number Organism ppc NP_418391 16131794 Escherichia coli ppcA AAB58883 28572162 Methylobacterium extorquens ppc ABB53270 80973080 Corynebacterium glutamicum

Pyruvate Kinase and Methylmalonyl-CoA Carboxyltransferase

An additional energetically efficient route to oxaloacetate from PEP requires two enzymatic activities: pyruvate kinase and methylmalonyl-CoA carboxytransferase. Pyruvate kinase catalyzes the ATP-generating conversion of PEP to pyruvate and is encoded by the PYK1 (Burke, J. Biol. Chem. 258:2193-2201 (1983)) and PYK2 (Boles et al., J. Bacteriol. 179:2987-2993 (1997)) genes in S. cerevisiae. In E. coli, this activity is catalyzed by the gene product of pykF and pykA. Methylmalonyl-CoA carboxytransferase catalyzes the conversion of pyruvate to oxaloacetate. Importantly, this reaction also simultaneously catalyzes the conversion of (S)-methylmalonyl-CoA to propionyl-CoA (see FIG. 22). An exemplary methylmalonyl-CoA carboxytransferase which is comprised of 1.3S, 5S, and 12S subunits can be found in Propionibacterium freudenreichii (Thornton et al., J. Bacteriol 175:5301-5308 (1993)). These proteins are identified below.

Protein GenBank ID GI Number Organism PYK1 NP_009362 6319279 Saccharomyces cerevisiae PYK2 NP_014992 6324923 Saccharomyces cerevisiae pykF NP_416191.1 16129632 Escherichia coli pykA NP_416368.1 16129807 Escherichia coli 1.3S subunit P02904 114847 Propionibacterium freudenreichii 5S subunit Q70AC7 62901478 Propionibacterium freudenreichii 12S subunit Q8GBW6 62901481 Propionibacterium freudenreichii

Pyruvate Kinase and Pyruvate Carboxylase

A combination of enzymes can convert PEP to oxaloacetate with a stoichiometry identical to that of PEP carboxylase. These enzymes are encoded by pyruvate kinase, PYK1 (Burke, J. Biol. Chem. 258:2193-2201 (1983)) or PYK2 (Boles et al., J. Bacteriol, 179:2987-2993 (1997)) and pyruvate carboxylase, PYC1 (Walker, Biochem. Biophys. Res. Commun. 176:1210-1217 (1991)) or PYC2 (Walker, Biochem. Biophys. Res. Commun. 176:1210-1217 (1991)). The latter proteins are identified below.

Protein GenBank ID GI Number Organism PYC1 NP_011453 6321376 Saccharomyces cerevisiae PYC2 NP_009777 6319695 Saccharomyces cerevisiae Pyc YP_890857.1 118470447 Mycobacterium smegmatis

Malate Dehydrogenase, Fumarase, Fumarate Reductase

Oxaloacetate can be converted to succinate by malate dehydrogenase, fumarase and fumarate reductase when the TCA cycle is operating in the reductive cycle. S. cerevisiae possesses three copies of malate dehydrogenase, MDH1 (McAlister-Henn, J. Bacteriol 169:5157-5166 (1987)) MDH2 (Minard, Mol. Cell. Biol. 11:370-380 (1991); and Gibson, J. Biol. Chem. 278:25628-25636 (2003)), and MDH3 (Steffan, J. Biol. Chem. 267:24708-24715 (1992)), which localize to the mitochondrion, cytosol, and peroxisome, respectively. S. cerevisiae contains one copy of a fumarase-encoding gene, FUM1, whose product localizes to both the cytosol and mitochondrion (Sass, J. Biol. Chem. 278:45109-45116 (2003)). Fumarate reductase is encoded by two soluble enzymes, FRDS1 (Enomoto, DNA. Res. 3:263-267 (1996)) and FRDS2 (Muratsubaki, Arch. Biochem. Biophys. 352:175-181 (1998)), which localize to the cytosol and promitochondrion, respectively, and are required for anaerobic growth on glucose (Arikawa, Microbiol Lett. 165:111-116 (1998)). E. coli is known to have an active malate dehydrogenase. It has three fumarases encoded by fumA, B and C, each one of which is active under different conditions of oxygen availability. The fumarate reductase in E. coli is composed of four subunits. These proteins are identified below.

Protein GenBank ID GI Number Organism MDH1 NP_012838 6322765 Saccharomyces cerevisiae MDH2 NP_014515 116006499 Saccharomyces cerevisiae MDH3 NP_010205 6320125 Saccharomyces cerevisiae FUM1 NP_015061 6324993 Saccharomyces cerevisiae FRDS1 P32614 418423 Saccharomyces cerevisiae FRDS2 NP_012585 6322511 Saccharomyces cerevisiae frdA NP_418578.1 16131979 Escherichia coli frdB NP_418577.1 16131978 Escherichia coli frdC NP_418576.1 16131977 Escherichia coli frdD NP_418475.1 16131877 Escherichia coli Mdh NP_417703.1 16131126 Escherichia coli FumA NP_416129.1 16129570 Escherichia coli FumB NP_418546.1 16131948 Escherichia coli FumC NP_416128.1 16129569 Escherichia coli

Further exemplary enzymes are found in several organisms including E. coli, Bacillus subtilis, and Rhizopus oryzae.

Protein GenBank ID GI number Organism mdh AAC76268.1 1789632 Escherichia coli mdh NP_390790.1 16079964 Bacillus subtilis MDH ADG65261.1 296011196 Rhizopus oryzae

Succinyl-CoA:3-Ketoacid-CoA Transferase

The conversion of succinate to succinyl-CoA is ideally carried by a transferase which does not require the direct consumption of an ATP or GTP. This type of reaction is common in a number of organisms. Perhaps the top candidate enzyme for this reaction step is succinyl-CoA:3-ketoacid-CoA transferase. This enzyme converts succinate to succinyl-CoA while converting a 3-ketoacyl-CoA to a 3-ketoacid. Exemplary succinyl-CoA:3:ketoacid-CoA transferases are present in Helicobacter pylori (Corthesy-Theulaz et al., J. Biol. Chem. 272:25659-25667 (1997)), Bacillus subtilis (Stols et al., Protein. Expr. Purif. 53:396-403 (2007)), and Homo sapiens (Fukao et al., Genomics, 68:144-151 (2000); and Tanaka, Mol. Hum. Reprod. 8:16-23 (2002)). These proteins are identified below.

Protein GenBank ID GI Number Organism HPAG1_0676 YP_627417 108563101 Helicobacter pylori HPAG1_0677 YP_627418 108563102 Helicobacter pylori ScoA NP_391778 16080950 Bacillus subtilis ScoB NP_391777 16080949 Bacillus subtilis OXCT1 NP_000427 4557817 Homo sapiens OXCT2 NP_071403 11545841 Homo sapiens

Succinyl-CoA: Acetyl-CoA Transferase

The conversion of succinate to succinyl-CoA can also be catalyzed by succinyl-CoA: Acetyl-CoA transferase. The gene product of cat1 of Clostridium kluyveri has been shown to exhibit succinyl-CoA: acetyl-CoA transferase activity (Sohling, J Bacteriol. 178:871-880 (1996)). In addition, the activity is present in Trichomonas vaginalis (van Grinsven et at, J. Biol. Chem. 283:1411-1418 (2008)) and Trypanosoma brucei (Riviere et al., J. Biol. Chem. 279:45337-45346 (2004)). These proteins are identified below.

Protein GenBank ID GI Number Organism cat1 P38946.1 729048 Clostridium kluyveri TVAG_395550 XP_001330176 123975034 Trichomonas vaginalis G3 Tb11.02.0290 XP_828352 71754875 Trypanosoma brucei

Succinyl-CoA Synthetase

The product of the LSC1 and LSC2 genes of S. cerevisiae and the sucC and sucD genes of E. coli naturally form a succinyl-CoA synthetase complex that catalyzes the formation of succinyl-CoA from succinate with the concomitant consumption of one ATP, a reaction which is reversible in vivo (Przybyla-Zawilask et al., Eur. J. Biochem. 258(2):736-743 (1998) and Buck et al., J. Gen. Microbiol. 132(6):1753-1762 (1986)). These proteins are identified below.

Protein GenBank ID GI Number Organism LSC1 NP_014785 6324716 Saccharomyces cerevisiae LSC2 NP_011760 6321683 Saccharomyces cerevisiae SucC NP_415256.1 16128703 Escherichia coli SucD AAC73823.1 1786949 Escherichia coli

Methylmalonyl-CoA Mutase

Succinyl-CoA can be converted into (R)-methylmalonyl-CoA by methylmalonyl-CoA mutase (MCM). In E. coli, the reversible adenosylcobalamin-dependant mutase participates in a three-step pathway leading to the conversion of succinate to propionate (Haller, Biochemistry 39:4622-9 (2000)). MCM is encoded by genes scpA in Escherichia coli (Haller, Biochemistry 39: 4622-4629 (2000); and Bobik, Anal. Bioanal. Chem. 375:344-349 (2003)) and mutA in Homo sapiens (Padovani, Biochemistry 45:9300-9306 (2006)). In several other organisms MCM contains alpha and beta subunits and is encoded by two genes. Exemplary gene candidates encoding the two-subunit protein are Propionibacterium fredenreichii sp. shermani mutA and mutB (Korotkova, J Biol Chem. 279:13652-13658 (2004)) and Methylobacterium extorquens mcmA and mcmB (Korotkova, J Biol Chem. 279:13652-13658 (2004)). These proteins are identified below.

Protein GenBank ID GI Number Organism ScpA NP_417392.1 16130818 Escherichia coli K12 MutA P22033.3 67469281 Homo sapiens MutA P11652.3 127549 Propionibacterium fredenreichii sp. shermanii MutB P11653.3 127550 Propionibacterium fredenreichii sp. shermanii mcmA Q84FZ1 75486201 Methylobacterium extorquens McmB Q6TMA2 75493131 Methylobacterium extorquens

Additional enzyme candidates identified based on high homology to the E. coli spcA gene product are identified below.

Protein GenBank ID GI Number Organism Sbm NP_838397.1 30064226 Shigella flexneri SARI_04585 ABX24358.1 160867735 Salmonella enterica YfreA_01000861 ZP_00830776.1 77975240 Yersinia frederiksenii

There further exists evidence that genes adjacent to the methylmalonyl-CoA mutase catalytic genes are also required for maximum activity. For example, it has been demonstrated that the meaB gene from M. extorquens forms a complex with methylmalonyl-CoA mutase, stimulates in vitro mutase activity, and possibly protects it from irreversible inactivation (Korotkova, J Biol Chem. 279:13652-13658 (2004)). The M. extorquens meaB gene product is highly similar to the product of the E. coli argK gene (BLASTp: 45% identity, e-value: 4e-67) which is adjacent to scpA on the chromosome. No sequence for a meaB homolog in P. freudenreichii is catalogued in GenBank. However, the Propionibacterium acnes KPA171202 gene product, YP_055310.1, is 51% identical to the M. extorquens meaB protein and its gene is also adjacent to the methylmalonyl-CoA mutase gene on the chromosome. These proteins are identified below.

Protein GenBank ID GI Number Organism ArgK AAC75955.1 1789285 Escherichia coli K12 KPA171202 YP_055310.1 50842083 Propionibacterium acnes MeaB 2QM8_B 158430328 Methylobacterium extorquens

Methylmalonyl-CoA Epimerase

Methylmalonyl-CoA epimerase (MMCE) is the enzyme that interconverts (R)-methylmalonyl-CoA and (S)-methylmalonyl-CoA. MMCE is an essential enzyme in the breakdown of odd-numbered fatty acids and of the amino acids valine, isoleucine, and methionine. Methylmalonyl-CoA epimerase is present in organisms such as Bacillus subtilis (YqjC) (Haller, Biochemistry. 39:4622-4629 (2000)), Homo sapiens (YqjC) (Fuller, Biochem. J 213:643-650 (1983)), Rattus norvegicus (Mcee) (Bobik, J Biol Chem. 276:37194-37198 (2001)), Propionibacterium shermanii (AF454511) (Haller, Biochemistry 39:4622-9 (2000); McCarthy, Structure 9:637-46 (2001) and (Fuller, Biochem. J 213:643-650 (1983)) and Caenorhabditis elegans (mmce) (Kuhnl et al., FEBS J 272:1465-1477 (2005)). The additional gene candidate, AE016877 in Bacillus cereus, has high sequence homology to the other characterized enzymes. MMCE activity is required if the employed methylmalonyl-CoA decarboxylase or methylmalonyl-CoA carboxytransferase requires the (S) stereoisomer of methylmalonyl-CoA. These proteins are identified below.

Protein GenBank ID GI Number Organism YqjC NP_390273 255767522 Bacillus subtilis MCEE Q96PE7.1 50401130 Homo sapiens Mcee_predicted NP_001099811.1 157821869 Rattus norvegicus AF454511 AAL57846.1 18042135 Propionibacterium fredenreichii sp. shermanii Mmce AAT92095.1 51011368 Caenorhabditis elegans AE016877 AAP08811.1 29895524 Bacillus cereus ATCC 14579

Methylmalonyl-CoA Decarboxylase

Methylmalonyl-CoA decarboxylase, is a biotin-independent enzyme that catalyzes the conversion of methylmalonyl-CoA to propionyl-CoA in E. coli (Benning, Biochemistry. 39:4630-4639 (2000); and Haller, Biochemistry. 39:4622-4629 (2000)). The stereo specificity of the E. coli enzyme was not reported, but the enzyme in Propionigenium modestum (Bott et al., Eur. J. Biochem. 250:590-599 (1997)) and Veillonella parvula (Huder, J. Biol. Chem. 268:24564-24571 (1993)) catalyzes the decarboxylation of the (S)-stereoisomer of methylmalonyl-CoA (Hoffmann, FEBS. Lett. 220:121-125 (1987). The enzymes from P. modestum and V. parvula are comprised of multiple subunits that not only decarboxylate (S)-methylmalonyl-CoA, but also create a pump that transports sodium ions across the cell membrane as a means to generate energy. These proteins are identified below.

Protein GenBank ID GI Number Organism YgfG NP_417394 90111512 Escherichia coli mmdA CAA05137 2706398 Propionigenium modestum mmdD CAA05138 2706399 Propionigenium modestum mmdC CAA05139 2706400 Propionigenium modestum mmdB CAA05140 2706401 Propionigenium modestum mmdA CAA80872 415915 Veillonella parvula mmdC CAA80873 415916 Veillonella parvula mmdE CAA80874 415917 Veillonella parvula mmdD CAA80875 415918 Veillonella parvula mmdB CAA80876 415919 Veillonella parvula

Example XVIII In Vivo Labeling Assay for Conversion of Methanol to CO₂

This example describes a functional methanol pathway in a microbial organism.

Strains with functional reductive TCA branch and pyruvate formate lyase deletion were grown aerobically in LB medium overnight, followed by inoculation of M9 high-seed media containing IPTG and aerobic growth for 4 hrs. These strains had methanol dehydrogenase/ACT pairs in the presence and absence of formaldehyde dehydrogenase or formate dehydrogenase. ACT is an activator protein (a Nudix hydrolase). At this time, strains were pelleted, resuspended in fresh M9 medium high-seed media containing 2% ¹³CH₃OH, and sealed in anaerobic vials. Head space was replaced with nitrogen and strains grown for 40 hours at 37° C. Following growth, headspace was analyzed for ¹³CO₂. Media was examined for residual methanol as well as BDO and byproducts. All constructs expressing methanol dehydrogenase (MeDH) mutants and MeDH/ACT pairs grew to slightly lower ODs than strains containing empty vector controls. This is likely due to the high expression of these constructs (Data not shown). One construct (2315/2317) displayed significant accumulation of labeled CO₂ relative to controls in the presence of FalDH, FDH or no coexpressed protein. This shows a functional MeOH pathway in E. coli and that the endogenous glutathione-dependent formaldehyde detoxification genes (frmAB) are sufficient to carry flux generated by the current MeDH/ACT constructs.

2315 is internal laboratory designation for the MeDH from Bacillus methanolicus MGA3 (GenBank Accession number: E1177596.1; GI number: 387585261), and 2317 is internal laboratory designation for the activator protein from the same organism (locus tag: MGA3_09170; GenBank Accession number: EIJ83380; GI number: 387591061).

Sequence analysis of the NADH-dependent MeDH from Bacillus methanolicus places the enzyme in the alcohol dehydrogenase family III. It does not contain any tryptophan residues, resulting in a low extinction coefficient (18,500 M⁻¹, cm⁻¹) and should be detected on SDS gels by Coomassie staining.

The enzyme has been characterized as a multisubunit complex built from 43 kDa subunits containing one Zn and 1-2 Mg atoms per subunit. Electron microscopy and sedimentation studies determined it to be a decamer, in which two rings with five-fold symmetry are stacked on top of each other (Vonck et al., J. Biol. Chem. 266:3949-3954, 1991). It is described to contain a tightly but not covalently bound cofactor and requires exogenous NAD⁺ as e⁻-acceptor to measure activity in vitro. A strong increase (10-40-fold) of in vitro activity was observed in the presence of an activator protein (ACT), which is a homodimer (21 kDa subunits) and contains one Zn and one Mg atom per subunit.

The mechanism of the activation was investigated by Kloosterman et al., J. Biol. Chem. 277:34785-34792, 2002, showing that ACT is a Nudix hydrolase and Hektor et al., J. Biol. Chem. 277:46966-46973, 2002, demonstrating that mutation of residue S97 to G or Tin MeDH changes activation characteristics along with the affinity for the cofactor. While mutation of residues G15 and D88 had no significant impact, a role of residue G13 for stability as well as of residues G95, D100, and K103 for the activity is suggested. Both papers together propose a hypothesis in which ACT cleaves MeDH-bound NAD⁺. MeDH retains AMP bound and enters an activated cycle with increased turnover.

The stoichiometric ratio between ACT and MeDH is not well defined in the literature. Kloosterman et al., supra determine the ratio of dimeric Act to decameric MeDH for full in vitro activation to be 10:1. In contrast, Arfman et al. J. Biol. Chem. 266:3955-3960, 1991 determined a ratio of 3:1 in vitro for maximum and a 1:6 ratio for significant activation, but observe a high sensitivity to dilution. Based on expression of both proteins in Bacillus, the authors estimate the ratio in vivo to be around 1:17.5.

However, our in vitro experiments with purified activator protein (2317A) and methanol dehydrogenase (2315A) showed the ratio of ACT to MeDH to be 10:1. This in vitro test was done with 5 M methanol, 2 mM NAD and 10 μM methanol dehydrogenase 2315A at pH 7.4.

Example XIX Improving Product Yields on Methanol Using Phosphoketolase-Dependent Cetyl-CoA Synthesis

Acetyl-CoA is the immediate precursor for the synthesis of isopropanol, fatty acyl-CoA molecules, and fatty acyl-ACP molecules as shown in FIGS. 2, 11, and 12. Phosphoketolase pathways make possible synthesis of acetyl-CoA without requiring decarboxylation of pyruvate (Bogorad et al, Nature, 2013, published online 29 Sep. 2013; United States Publication 2006-0040365), which thereby provides higher yields of fatty alcohols, fatty acids, fatty aldehydes, and isopropanol from carbohydrates and methanol than the yields attainable without phosphoketolase enzymes.

For example, synthesis of an exemplary fatty alcohol, dodecanol, from methanol using methanol dehydrogenase (step A of FIG. 1), a formaldehyde assimilation pathway (steps B, C, D of FIG. 1), the pentose phosphate pathway, and glycolysis can provide a maximum theoretical yield of 0.0556 mole dodecanol/mole methanol.

18CH₄O+9O₂→C₁₂H₂₆O+23H₂O+6CO₂

However, if these pathways are combined with a phosphoketolase pathway (steps T, U, V, W, X of FIG. 1), a maximum theoretical yield of 0.0833 mole dodecanol/mole methanol can be obtained if we assume that the pathway is not required to provide net generation of ATP for cell growth and maintenance requirements.

12CH₄O→C₁₂H₂₆O+11H₂O

ATP for energetic requirements can be synthesized, at the expense of lowering the maximum theoretical product yield, by oxidizing methanol to CO₂ using several combinations of enzymes depicted in FIG. 10, glycolysis, the TCA cycle, the pentose phosphate pathway, and oxidative phosphorylation.

Similarly, synthesis of isopropanol from methanol using methanol dehydrogenase (step A of FIG. 1), a formaldehyde assimilation pathway (steps B, C, D of FIG. 1), the pentose phosphate pathway and glycolysis can provide a maximum theoretical yield of 0.1667 mole isopropanol/mole methanol.

6CH₄O+4.5O₂→C₃H₈O+8H₂O+3CO₂

However, if these pathways are applied in combination with a phosphoketolase pathway (steps T, U, V, W, X of FIG. 1), a maximum theoretical yield of 0.250 mole isopropanol/mole methanol can be obtained.

4CH₄O+1.5O₂→C₃H₈O+4H₂O+CO₂

The overall pathway is ATP and redox positive enabling synthesis of both ATP and NAD(P)H from conversion of MeOH to isopropanol. Additional ATP can be synthesized, at the expense of lowering the maximum theoretical product yield, by oxidizing methanol to CO₂ using several combinations of enzymes depicted in FIG. 10, glycolysis, the TCA cycle, the pentose phosphate pathway, and oxidative phosphorylation.

Example XX Improving Product Yields on Carbohydrates Using Phosphoketolase-Dependent Acetyl-CoA Synthesis and Exogenous Reducing Equivalents

The theoretical yield of fatty acyl-CoA molecules, fatty acyl-ACP molecules, and isopropanol from carbohydrates including but not limited to glucose, glycerol, sucrose, fructose, xylose, arabinose, and galactose, can also be enhanced by phosphoketolase enzymes, particularly when reducing equivalents are provided by an exogenous source such as hydrogen or methanol. This is because phosphoketolase enzymes provide acetyl-CoA synthesis with 100% carbon conversion efficiency (e.g., 3 acetyl-CoA's per glucose, 2.5 acetyl-CoA's per xylose, 1.5 acetyl-CoA's per glycerol).

For example, synthesis of an exemplary fatty alcohol, dodecanol, from glucose in the absence of phosphoketolase enzymes can reach a maximum theoretical dodecanol yield of 0.3333 mole dodecanol/mole glucose.

3C₆H₁₂O₆→C₁₂H₂₆O+5H₂O+6CO₂

However, if enzyme steps T, U, V, W, X of FIG. 1 are applied in combination with glycolysis, the pentose phosphate pathway, and an external redox source (e.g., methanol, hydrogen) using the pathways shown in FIG. 10, the maximum theoretical yield can be increased to 0.5000 mole dodecanol/mole glucose.

2C₆H₁₂O₆+4CH₄O→C₁₂H₂₆O+7H₂O+4CO₂

This assumes that the pathway is not required to provide net generation of ATP for cell growth and maintenance requirements. ATP for energetic requirements can be synthesized by oxidizing additional methanol to CO₂ using several combinations of enzymes depicted in FIG. 10.

Similarly, synthesis of isopropanol from glucose in the absence of phosphoketolase enzymes can achieve a maximum theoretical isopropanol yield of 1.000 mole isopropanol/mole glucose.

C₆H₁₂O₆+1.5O₂→C₃H₈O+2H₂O+3CO₂

However, if enzyme steps T, U, V, W, X of FIG. 1 are applied in combination with glycolysis and the pentose phosphate pathway, the maximum theoretical yield can be increased to 1.333 mole isopropanol/mole glucose.

C₆H₁₂O₆→1.333C₃H₈O+0.667H₂O+2CO₂

If enzyme steps T, U, V, W, X of FIG. 1 are applied in combination with glycolysis, the pentose phosphate pathway, and external redox source (e.g., methanol, hydrogen) using the pathways shown in FIG. 10, the maximum theoretical yield can be increased to 1.500 mole isopropanol/mole glucose.

C₆H₁₂O₆+0.5CH₄O→1.5C₃H₈O+H₂O+2CO₂

Example XXI Phosphoketolase-Dependent Acetyl-CoA Synthesis Enzymes

This Example provides genes that can be used for enhancing carbon flux through acetyl-CoA using phosphoketolase enzymes.

FIG. 1, Step T—Fructose-6-phosphate phosphoketolase

Conversion of fructose-6-phosphate and phosphate to acetyl-phosphate and erythrose-5-phosphate can be carried out by fructose-6-phosphate phosphoketolase (EC 4.1.2.22). Conversion of fructose-6-phosphate and phosphate to acetyl-phosphate and erythrose-5-phosphate is one of the key reactions in the Bifidobacterium shunt. There is evidence for the existence of two distinct phosphoketolase enzymes in bifidobacteria (Sgorbati et al, 1976, Antonie Van Leeuwenhoek, 42(1-2) 49-57; Grill et al, 1995, Curr Microbiol, 31(1); 49-54). The enzyme from Bifidobacterium dentium appeared to be specific solely for fructose-6-phosphate (EC: 4.1.2.22) while the enzyme from Bifidobacterium pseudolongum subsp. globosum is able to utilize both fructose-6-phosphate and D-xylulose 5-phosphate (EC: 4.1.2.9) (Sgorbati et al, 1976, Antonie Van Leeuwenhoek, 42(1-2) 49-57). The enzyme encoded by the xfp gene, originally discovered in Bifidobacterium animalis lactis, is the dual-specificity enzyme (Meile et al., 2001, J Bacteriol, 183, 2929-2936; Yin et al, 2005, FEMS Microbiol Lett, 246(2); 251-257). Additional phosphoketolase enzymes can be found in Leuconostoc mesenteroides (Lee et al, Biotechnol Left. 2005 June; 27(12):853-8), Clostridium acetobutylicum ATCC 824 (Servinsky et al, Journal of Industrial Microbiology & Biotechnology, 2012, 39, 1859-1867), Aspergillus nidulans (Kocharin et al, 2013, Biotechnol Bioeng, 110(8), 2216-2224; Papini, 2012, Appl Microbiol Biotechnol, 95 (4), 1001-1010), Bifidobacterium breve (Suziki et al, 2010, Acta Crystallogr Sect F Struct Biol Cryst Commun., 66(Pt 8):941-3), Lactobacillus paraplantarum (Jeong et al, 2007, J Microbiol Biotechnol, 17(5), 822-9).

Protein GenBank ID GI NO. Organism xfp YP_006280131.1 386867137 Bifidobacterium animalis lactis xfp AAV66077.1 55818565 Leuconostoc mesenteroides CAC1343 NP_347971.1 15894622 Clostridium acetobutylicum ATCC 824 xpkA CBF76492.1 259482219 Aspergillus nidulans xfp WP_003840380.1 489937073 Bifidobacterium dentium ATCC 27678 xfp AAR98788.1 41056827 Bifidobacterium pseudolongum subsp. globosum xfp WP_022857642.1 551237197 Bifidobacterium pseudolongum subsp. globosum xfp ADF97524.1 295314695 Bifidobacterium breve xfp AAQ64626.1 34333987 Lactobacillus paraplantarum

FIG. 1, Step U—Xylulose-5-phosphate phosphoketolase

Conversion of xylulose-5-phosphate and phosphate to acetyl-phosphate and glyceraldehyde-3-phosphate can be carried out by xylulose-5-phosphate phosphoketolase (EC 4.1.2.9). There is evidence for the existence of two distinct phosphoketolase enzymes in bifidobacteria (Sgorbati et al, 1976, Antonie Van Leeuwenhoek, 42(1-2) 49-57; Grill et al, 1995, Curr Microbiol, 31(0; 49-54). The enzyme from Bifidobacterium dentium appeared to be specific solely for fructose-6-phosphate (EC: 4.1.2.22) while the enzyme from Bifidobacterium pseudolongum subsp. globosum is able to utilize both fructose-6-phosphate and D-xylulose 5-phosphate (EC: 4.1.2.9) (Sgorbati et al, 1976, Antonie Van Leeuwenhoek, 42(1-2) 49-57). Many characterized enzymes have dual-specificity for xylulose-5-phosphate and fructose-6-phosphate. The enzyme encoded by the xfp gene, originally discovered in Bifidobacterium animalis lactis, is the dual-specificity enzyme (Meile et al., 2001, J Bacteriol, 183, 2929-2936; Yin et al, 2005, FEMS Microbiol Lett, 246(2); 251-257). Additional phosphoketolase enzymes can be found in Leuconostoc mesenteroides (Lee et al, Biotechnol Left. 2005 June; 27(12):853-8), Clostridium acetobutylicum ATCC 824 (Servinsky et al, Journal of Industrial Microbiology & Biotechnology, 2012, 39, 1859-1867), Aspergillus nidulans (Kocharin et al, 2013, Biotechnol Bioeng, 110(8), 2216-2224; Papini, 2012, Appl Microbiol Biotechnol, 95 (4), 1001-1010), Bifidobacterium breve (Suziki et al, 2010, Acta Crystallogr Sect F Struct Biol Cryst Commun., 66(Pt 8):941-3), and Lactobacillus paraplantarum (Jeong et al, 2007, J Microbiol Biotechnol, 17(5), 822-9).

Protein GenBank ID GI NO. Organism xfp YP_006280131.1 386867137 Bifidobacterium animalis lactis xfp AAV66077.1 55818565 Leuconostoc mesenteroides CAC1343 NP_347971.1 15894622 Clostridium acetobutylicum ATCC 824 xpkA CBF76492.1 259482219 Aspergillus nidulans xfp AAR98788.1 41056827 Bifidobacterium pseudolongum subsp. globosum xfp WP_022857642.1 551237197 Bifidobacterium pseudolongum subsp. globosum xfp ADF97524.1 295314695 Bifidobacterium breve xfp AAQ64626.1 34333987 Lactobacillus paraplantarum

FIG. 1, Step V—Phosphotransacetylase

The formation of acetyl-CoA from acetyl-phosphate can be catalyzed by phosphotransacetylase (EC 2.3.1.8). The pta gene from E. coli encodes an enzyme that reversibly converts acetyl-CoA into acetyl-phosphate (Suzuki, T., Biochim. Biophys. Acta 191:559-569 (969)). Additional acetyltransferase enzymes have been characterized in Bacillus subtilis (Rado and Hoch, Biochim. Biophys. Acta 321:114-125 (1973), Clostridium kluyveri (Stadtman, E., Methods Enzymol. 1:5896-599 (1955), and Thermotoga maritima (Bock et al., J. Bacteriol. 181:1861-1867 (1999)). This reaction can also be catalyzed by some phosphotranbutyrylase enzymes (EC 2.3.1.19), including the ptb gene products from Clostridium acetobutylicum (Wiesenbom et al., App. Environ. Microbiol. 55:317-322 (1989); Walter et al., Gene 134:107-111 (1993)). Additional ptb genes are found in butyrate-producing bacterium L2-50 (Louis et al., J. Bacteriol. 186:2099-2106 (2004) and Bacillus megaterium (Vazquez et al., Curr. Microbiol. 42:345-349 (2001). Homologs to the E. coli pta gene exist in several other organisms including Salmonella enterica and Chlamydomonas reinhardtii.

Protein GenBank ID GI Number Organism Pta NP_416800.1 71152910 Escherichia coli Pta P39646 730415 Bacillus subtilis Pta A5N801 146346896 Clostridium kluyveri Pta Q9X0L4 6685776 Thermotoga maritime Ptb NP_349676 34540484 Clostridium acetobutylicum Ptb AAR19757.1 38425288 butyrate-producing bacterium L2-50 Ptb CAC07932.1 10046659 Bacillus megaterium Pta NP_461280.1 16765665 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 PAT2 XP_001694504.1 159472743 Chlamydomonas reinhardtii PAT1 XP_001691787.1 159467202 Chlamydomonas reinhardtii

FIG. 1, Step W—Acetate kinase

Acetate kinase (EC 2.7.2.1) can catalyze the reversible ATP-dependent phosphorylation of acetate to acetylphosphate. Exemplary acetate kinase enzymes have been characterized in many organisms including E. coli, Clostridium acetobutylicum and Methanosarcina thermophila (Ingram-Smith et al., J. Bacteriol. 187:2386-2394 (2005); Fox and Roseman, J. Biol. Chem. 261:13487-13497 (1986); Winzer et al., Microbiology 143 (Pt 10):3279-3286 (1997)). Acetate kinase activity has also been demonstrated in the gene product of E. coli purT (Marolewski et al., Biochemistry 33:2531-2537 (1994). Some butyrate kinase enzymes (EC 2.7.2.7), for example buk1 and buk2 from Clostridium acetobutylicum, also accept acetate as a substrate (Hartmanis, M. G., J. Biol. Chem. 262:617-621 (1987)). Homologs exist in several other organisms including Salmonella enterica and Chlamydomonas reinhardtii.

Protein GenBank ID GI Number Organism ackA NP_416799.1 16130231 Escherichia coli Ack AAB18301.1 1491790 Clostridium acetobutylicum Ack AAA72042.1 349834 Methanosarcina thermophila purT AAC74919.1 1788155 Escherichia coli buk1 NP_349675 15896326 Clostridium acetobutylicum buk2 Q97II1 20137415 Clostridium acetobutylicum ackA NP_461279.1 16765664 Salmonella typhimurium ACK1 XP_001694505.1 159472745 Chlamydomonas reinhardtii ACK2 XP_001691682.1 159466992 Chlamydomonas reinhardtii

FIG. 1, Step X—Acetyl-CoA Transferase, Synthetase, or Ligase

The acylation of acetate to acetyl-CoA can be catalyzed by enzymes with acetyl-CoA synthetase, ligase or transferase activity. Two enzymes that can catalyze this reaction are AMP-forming acetyl-CoA synthetase or ligase (EC 6.2.1.1) and ADP-forming acetyl-CoA synthetase (EC 6.2.1.13). AMP-forming acetyl-CoA synthetase (ACS) is the predominant enzyme for activation of acetate to acetyl-CoA. Exemplary ACS enzymes are found in E. coli (Brown et al., J. Gen. Microbiol. 102:327-336 (1977)), Ralstonia eutropha (Priefert and Steinbuchel, J. Bacteriol. 174:6590-6599 (1992)), Methanothermobacter thermautotrophicus (Ingram-Smith and Smith, Archaea 2:95-107 (2007)), Salmonella enterica (Gulick et al., Biochemistry 42:2866-2873 (2003)) and Saccharomyces cerevisiae (Jogl and Tong, Biochemistry 43:1425-1431 (2004)). ADP-forming acetyl-CoA synthetases are reversible enzymes with a generally broad substrate range (Musfeldt and Schonheit, J. Bacteriol. 184:636-644 (2002)). Two isozymes of ADP-forming acetyl-CoA synthetases are encoded in the Archaeoglobus fulgidus genome by are encoded by AF1211 and AF1983 (Musfeldt and Schonheit, supra (2002)). The enzyme from Haloarcula marismortui (annotated as a succinyl-CoA synthetase) also accepts acetate as a substrate and reversibility of the enzyme was demonstrated (Brasen and Schonheit, Arch. Microbiol. 182:277-287 (2004)). The ACD encoded by PAE3250 from hyperthermophilic crenarchaeon Pyrobaculum aerophilum showed the broadest substrate range of all characterized ACDs, reacting with acetate, isobutyryl-CoA (preferred substrate) and phenylacetyl-CoA (Brasen and Schonheit, supra (2004)). Directed evolution or engineering can be used to modify this enzyme to operate at the physiological temperature of the host organism. The enzymes from A. fulgidus, H. marismortui and P. aerophilum have all been cloned, functionally expressed, and characterized in E. coli (Brasen and Schonheit, supra (2004); Musfeldt and Schonheit, supra (2002)). Additional candidates include the succinyl-CoA synthetase encoded by sucCD in E. coli (Buck et al., Biochemistry 24:6245-6252 (1985)) and the acyl-CoA ligase from Pseudomonas putida (Fernandez-Valverde et al., Appl. Environ. Microbiol. 59:1149-1154 (1993)). The aforementioned proteins are shown below.

Protein GenBank ID GI Number Organism Acs AAC77039.1 1790505 Escherichia coli acoE AAA21945.1 141890 Ralstonia eutropha acs1 ABC87079.1 86169671 Methanothermobacter thermautotrophicus acs1 AAL23099.1 16422835 Salmonella enterica ACS1 Q01574.2 257050994 Saccharomyces cerevisiae AF1211 NP_070039.1 11498810 Archaeoglobus fulgidus AF1983 NP_070807.1 11499565 Archaeoglobus fulgidus Scs YP_135572.1 55377722 Haloarcula marismortui PAE3250 NP_560604.1 18313937 Pyrobaculum aerophilum str. IM2 sucC NP_415256.1 16128703 Escherichia coli sucD AAC73823.1 1786949 Escherichia coli paaF AAC24333.2 22711873 Pseudomonas putida

An acetyl-CoA transferase that can utilize acetate as the CoA acceptor is acetoacetyl-CoA transferase, encoded by the E. coli atoA (alpha subunit) and atoD (beta subunit) genes (Vanderwinkel et al., Biochem. Biophys. Res Commun. 33:902-908 (1968); Korolev et al., Acta Crystallogr. D Biol Crystallogr. 58:2116-2121 (2002)). This enzyme has also been shown to transfer the CoA moiety to acetate from a variety of branched and linear acyl-CoA substrates, including isobutyrate (Matthies et al., Appl Environ Microbiol 58:1435-1439 (1992)), valerate (Vanderwinkel et al., supra) and butanoate (Vanderwinkel et al., supra) Similar enzymes exist in Corynebacterium glutamicum ATCC 13032 (Duncan et al., Appl Environ Microbiol 68:5186-5190 (2002)), Clostridium acetobutylicum (Cary et al., Appl Environ Microbiol 56:1576-1583 (1990)), and Clostridium saccharoperbutylacetonicum (Kosaka et al., Biosci. Biotechnol Biochem. 71:58-68 (2007)). These proteins are identified below.

Protein GenBank ID GI Number Organism atoA P76459.1 2492994 Escherichia coli K12 atoD P76458.1 2492990 Escherichia coli K12 actA YP_226809.1 62391407 Corynebacterium glutamicum ATCC 13032 cg0592 YP_224801.1 62389399 Corynebacterium glutamicum ATCC 13032 ctfA NP_149326.1 15004866 Clostridium acetobutylicum ctfB NP_149327.1 15004867 Clostridium acetobutylicum ctfA AAP42564.1 31075384 Clostridium saccharoperbutylacetonicum ctfB AAP42565.1 31075385 Clostridium saccharoperbutylacetonicum

Additional exemplary acetyl-CoA transferase candidates are catalyzed by the gene products of cat1, cat2, and cat3 of Clostridium kluyveri which have been shown to exhibit succinyl-CoA, 4-hydroxybutyryl-CoA, and butyryl-CoA transferase activity, respectively (Seedorf et al., supra; Sohling et al., Eur. J Biochem. 212:121-127 (1993); Sohling et al., J Bacteriol. 178:871-880 (1996)) Similar CoA transferase activities are also present in Trichomonas vaginalis (van Grinsven et al., J. Biol. Chem. 283:1411-1418 (2008)) and Trypanosoma brucei (Riviere et al., J. Biol. Chem. 279:45337-45346 (2004)). These proteins are identified below.

Protein GenBank ID GI Number Organism cat1 P38946.1 729048 Clostridium kluyveri cat2 P38942.2 172046066 Clostridium kluyveri cat3 EDK35586.1 146349050 Clostridium kluyveri TVAG_395550 XP_001330176 123975034 Trichomonas vaginalis G3 Tb11.02.0290 XP_828352 71754875 Trypanosoma brucei

Example XXII Attenuation or Disruption of Endogenous Enzymes

This example provides endogenous enzyme targets for attenuation or disruption that can be used for enhancing carbon flux through methanol dehydrogenase and formaldehyde assimilation pathways.

DHA Kinase

Methylotrophic yeasts typically utilize a cytosolic DHA kinase to catalyze the ATP-dependent activation of DHA to DHAP. DHAP together with G3P is combined to form fructose-1,6-bisphosphate (FBP) by FBP aldolase. FBP is then hydrolyzed to F6P by fructose bisphosphatase. The net conversion of DHA and G3P to F6P by this route is energetically costly (1 ATP) in comparison to the F6P aldolase route, described above and shown in FIG. 1. DHA kinase also competes with F6P aldolase for the DHA substrate. Attenuation of endogenous DHA kinase activity will thus improve the energetics of formaldehyde assimilation pathways, and also increase the intracellular availability of DHA for DHA synthase. DHA kinases of Saccharomyces cerevisiae, encoded by DAK1 and DAK2, enable the organism to maintain low intracellular levels of DHA (Molin et al, J Biol Chem 278:1415-23 (2003)). In methylotrophic yeasts DHA kinase is essential for growth on methanol (Luers et al, Yeast 14:759-71 (1998)). The DHA kinase enzymes of Hansenula polymorpha and Pichia pastoris are encoded by DAK (van der Klei et al, Curr Genet 34:1-11 (1998); Luers et al, supra). DAK enzymes in other organisms can be identified by sequence similarity to known enzymes.

Protein GenBank ID GI Number Organism DAK1 NP_013641.1 6323570 Saccharomyces cerevisiae DAK2 NP_116602.1 14318466 Saccharomyces cerevisiae DAK AAC27705.1 3171001 Hansenula polymorpha DAK AAC39490.1 3287486 Pichia pastoris DAK2 XP_505199.1 50555582 Yarrowia lipolytica

Methanol Oxidase

Attenuation of redox-inefficient endogenous methanol oxidizing enzymes, combined with increased expression of a cytosolic NADH-dependent methanol dehydrogenase, will enable redox-efficient oxidation of methanol to formaldehyde in the cytosol. Methanol oxidase, also called alcohol oxidase (EC 1.1.3.13), catalyzes the oxygen-dependent oxidation of methanol to formaldehyde and hydrogen peroxide. In eukaryotic organisms, alcohol oxidase is localized in the peroxisome. Exemplary methanol oxidase enzymes are encoded by AOD of Candida boidinii (Sakai and Tani, Gene 114:67-73 (1992)); and AOX of H. polymorpha, P. methanolica and P. pastoris (Ledeboer et al, Nucl Ac Res 13:3063-82 (1985); Koutz et al, Yeast 5:167-77 (1989); Nakagawa et al, Yeast 15:1223-1230 (1999)).

Protein GenBank ID GI Number Organism AOX2 AAF02495.1 6049184 Pichia methanolica AOX1 AAF02494.1 6049182 Pichia methanolica AOX1 AAB57849.1 2104961 Pichia pastoris AOX2 AAB57850.1 2104963 Pichia pastoris AOX P04841.1 113652 Hansenula polymorpha AOD1 Q00922.1 231528 Candida boidinii AOX1 AAQ99151.1 37694459 Ogataea pini

PQQ-Dependent Methanol Dehydrogenase

PQQ-dependent methanol dehydrogenase from M. extorquens (mxaIF) uses cytochrome as an electron carrier (Nunn et al, Nucl Acid Res 16:7722 (1988)). Methanol dehydrogenase enzymes of methanotrophs such as Methylococcus capsulatis function in a complex with methane monooxygenase (MMO) (Myronova et al, Biochem 45:11905-14 (2006)). Note that of accessory proteins, cytochrome CL and PQQ biosynthesis enzymes are needed for active methanol dehydrogenase. Attenuation of one or more of these required accessory proteins, or retargeting the enzyme to a different cellular compartment, would also have the effect of attenuating PQQ-dependent methanol dehydrogenase activity.

Protein GenBank ID GI Number Organism MCA0299 YP_112833.1 53802410 Methylococcus capsulatis MCA0782 YP_113284.1 53804880 Methylococcus capsulatis mxaI YP_002965443.1 240140963 Methylobacterium extorquens mxaF YP_002965446.1 240140966 Methylobacterium extorquens

DHA Synthase and Other Competing Formaldehyde Assimilation and Dissimilation Pathways

Carbon-efficient formaldehyde assimilation can be improved by attenuation of competing formaldehyde assimilation and dissimilation pathways. Exemplary competing assimilation pathways in eukaryotic organisms include the peroxisomal dissimilation of formaldehyde by DHA synthase, and the DHA kinase pathway for converting DHA to F6P, both described herein. Exemplary competing endogenous dissimilation pathways include one or more of the enzymes shown in FIG. 1.

Methylotrophic yeasts normally target selected methanol assimilation and dissimilation enzymes to peroxisomes during growth on methanol, including methanol oxidase, DHA synthase and S-(hydroxymethyl)-glutathione synthase (see review by Yurimoto et al, supra). The peroxisomal targeting mechanism comprises an interaction between the peroxisomal targeting sequence and its corresponding peroxisomal receptor (Lametschwandtner et al, J Biol Chem 273:33635-43 (1998)). Peroxisomal methanol pathway enzymes in methylotrophic organisms contain a PTS1 targeting sequence which binds to a peroxisomal receptor, such as Pex5p in Candida boidinii (Horiguchi et al, J Bacteriol 183:6372-83 (2001)). Disruption of the PTS1 targeting sequence, the Pex5p receptor and/or genes involved in peroxisomal biogenesis would enable cytosolic expression of DHA synthase, S-(hydroxymethyl)-glutathione synthase or other methanol-inducible peroxisomal enzymes. PTS1 targeting sequences of methylotrophic yeast are known in the art (Horiguchi et al, supra). Identification of peroxisomal targeting sequences of unknown enzymes can be predicted using bioinformatic methods (eg. Neuberger et al, J Mol Biol 328:581-92 (2003))).

Example XXIII Methanol Assimilation Via Methanol Dehydrogenase and the Ribulose Monophosphate Pathway

This example shows that co-expression of an active methanol dehydrogenase (MeDH) and the enzymes of the Ribulose Monophosphate (RuMP) pathway can effectively assimilate methanol derived carbon.

An experimental system was designed to test the ability of a MeDH in conjunction with the enzymes H6P synthase (HPS) and 6-phospho-3-hexuloisomerase (PHI) of the RuMP pathway to assimilate methanol carbon into the glycolytic pathway and the TCA cycle. Escherichia coli strain ECh-7150 (ΔlacIA, ΔpflB, ΔptsI, ΔPpckA(pckA), ΔPglk(g1k), glk::glfB, ΔhycE, ΔfrmR, ΔfrmA, ΔfrmB) was constructed to remove the glutathione-dependent formaldehyde detoxification capability encoded by the FrmA and FrmB enzyme. This strain was then transformed with plasmid pZA23S variants that either contained or lacked gene 2616A encoding a fusion of the HPS and PHI enzymes. These two transformed strains were then each transformed with pZS*13S variants that contained gene 2315L (encoding an active MeDH), or gene 2315 RIP2 (encoding a catalytically inactive MeDH), or no gene insertion. Genes 2315 and 2616 are internal nomenclatures for NAD-dependent methanol dehydrogenase from Bacillus methanolicus MGA3 and 2616 is a fused phs-hpi constructs as described in Orita et al. (2007) Appl Microbiol Biotechnol 76:439-45.

The six resulting strains were aerobically cultured in quadruplicate, in 5 ml minimal medium containing 1% arabinose and 0.6 M 13C-methanol as well as 100 ug/ml carbenicillin and 25 μg/ml kanamycin to maintain selection of the plasmids, and 1 mM IPTG to induce expression of the methanol dehydrogenase and HPS-PHI fusion enzymes. After 18 hours incubation at 37° C., the cell density was measured spectrophotometrically at 600 nM wavelength and a clarified sample of each culture medium was submitted for analysis to detect evidence of incorporation of the labeled methanol carbon into TCA-cycle derived metabolites. The label can be further enriched by deleting the gene araD that competes with ribulose-5-phosphate.

¹³C carbon derived from labeled methanol provided in the experiment was found to be significantly enriched in the metabolites pyruvate, lactate, succinate, fumarate, malate, glutamate and citrate, but only in the strain expressing both catalytically active MeDH 2315L and the HPS-PHI fusion 2616A together (data not shown). Moreover, this strain grew significantly better than the strain expressing catalytically active MeDH but lacking expression of the HPS-PHI fusion (data not shown), suggesting that the HPS-PHI enzyme is capable of reducing growth inhibitory levels of formaldehyde that cannot be detoxified by other means in this strain background. These results show that co-expression of an active MeDH and the enzymes of the RuMP pathway can effectively assimilate methanol derived carbon and channel it into TCA-cycle derived products.

Throughout this application various publications have been referenced. The disclosures of these publications in their entireties, including GenBank and GI number publications, are hereby incorporated by reference in this application in order to more fully describe the state of the art to which this invention pertains. Although the invention has been described with reference to the examples provided above, it should be understood that various modifications can be made without departing from the spirit of the invention. 

What is claimed is:
 1. A non-naturally occurring microbial organism having: (i) a formaldehyde fixation pathway; (ii) a formate assimilation pathway; and/or (iii) a methanol metabolic pathway, and a malonyl-CoA independent fatty acyl-CoA elongation (MI-FAE) cycle and/or a malonyl-CoA dependent fatty acyl-CoA elongation (MD-FAE) cycle in combination with a termination pathway, wherein said formaldehyde fixation pathway comprises: (1) 1B and 1C; (2) 1D; or (3) 1D and 1Z, wherein 1B is a 3-hexulose-6-phosphate synthase, wherein 1C is a 6-phospho-3-hexuloisomerase, wherein 1D is a dihydroxyacetone synthase, wherein 1Z is a fructose-6-phosphate aldolase, wherein said formate assimilation pathway comprises a pathway selected from: (4) 1E; (5) 1F, and 1G; (6) 1H, 1I, 1J, and 1K; (7) 1H, 1I, 1J, 1L, 1M, and 1N; (8) 1E, 1H, 1I, 1J, 1L, 1M, and 1N; (9) 1F, 1G, 1H, 1I, 1J, 1L, 1M, and 1N; (10) 1K, 1H, 1I, 1J, 1L, 1M, and 1N; and (11) 1H, 1I, 1J, 1O, and 1P, wherein 1E is a formate reductase, 1F is a formate ligase, a formate transferase, or a formate synthetase, wherein 1G is a formyl-CoA reductase, wherein 1H is a formyltetrahydrofolate synthetase, wherein 1I is a methenyltetrahydrofolate cyclohydrolase, wherein 1J is a methylenetetrahydrofolate dehydrogenase, wherein 1K is a formaldehyde-forming enzyme or spontaneous, wherein 1L is a glycine cleavage system, wherein 1M is a serine hydroxymethyltransferase, wherein 1N is a serine deaminase, wherein 1O is a methylenetetrahydrofolate reductase, wherein 1P is an acetyl-CoA synthase, wherein said methanol metabolic pathway comprises a pathway selected from: (12) 10J; (13) 10A; (14) 10A and 10B; (15) 10A, 10B and 10C; (16) 10J, 10K and 10C; (17) 10J, 10M, and 10N; (18) 10J and 10L; (19) 10J, 10L, and 10G; (20) 10J, 10L, and 10I; (21) 10A, 10B, 10C, 10D, and 10E; (22) 10A, 10B, 10C, 10D, and 10F; (23) 10J, 10K, 10C, 10D, and 10E; (24) 10J, 10K, 10C, 10D, and 10F; (25) 10J, 10M, 10N, and 10O; (26) 10A, 10B, 10C, 10D, 10E, and 10G; (27) 10A, 10B, 10C, 10D, 10F, and 10G; (28) 10J, 10K, 10C, 10D, 10E, and 10G; (29) 10J, 10K, 10C, 10D, 10F, and 10G; (30) 10J, 10M, 10N, 10O, and 10G; (31) 10A, 10B, 10C, 10D, 10E, and 10I; (32) 10A, 10B, 10C, 10D, 10F, and 10I; (33) 10J, 10K, 10C, 10D, 10E, and 10I; (34) 10J, 10K, 10C, 10D, 10F, and 10I; and (35) 10J, 10M, 10N, 10O, and 10I, wherein 10A is a methanol methyltransferase, wherein 10B is a methylenetetrahydrofolate reductase, wherein 10C is a methylenetetrahydrofolate dehydrogenase, wherein 10D is a methenyltetrahydrofolate cyclohydrolase, wherein 10E is a formyltetrahydrofolate deformylase, wherein 10F is a formyltetrahydrofolate synthetase, wherein 10G is a formate hydrogen lyase, wherein 10I is a formate dehydrogenase, wherein 10J is a methanol dehydrogenase, wherein 10K is a formaldehyde activating enzyme or spontaneous, wherein 10L is a formaldehyde dehydrogenase, wherein 10M is a S-(hydroxymethyl)glutathione synthase or spontaneous, wherein 10N is a glutathione-dependent formaldehyde dehydrogenase, wherein 10O is a S-formylglutathione hydrolase, wherein said MI-FAE cycle comprises one or more thiolase, one or more 3-oxoacyl-CoA reductase, one or more 3-hydroxyacyl-CoA dehydratase, and one or more enoyl-CoA reductase, wherein said MD-FAE cycle comprises one or more elongase, one or more 3-oxoacyl-CoA reductase, one or more 3-hydroxyacyl-CoA dehydratase, and one or more enoyl-CoA reductase, wherein said termination pathway comprises a pathway selected from: (36) 2H; (37) 2K and 2L; (38) 2E and 2N; (39) 2K, 2J, and 2N; (40) 2E; (41) 2K and 2J; (42) 2H and 2N; (43) 2K, 2L, and 2N; (44) 2E and 2F; (45) 2K, 2J, and 2F; (46) 2H, 2N, and 2F; (47) 2K, 2L, 2N, and 2F; (48) 2G; and (49) 2P, wherein 2E is an acyl-CoA reductase (aldehyde forming), wherein 2F is an alcohol dehydrogenase, wherein 2G is an acyl-CoA reductase (alcohol forming), wherein 2H is an acyl-CoA hydrolase, acyl-CoA transferase or acyl-CoA synthase, wherein 2J is an acyl-ACP reductase, wherein 2K is an acyl-CoA:ACP acyltransferase, wherein 2L is a thioesterase, wherein 2N is an aldehyde dehydrogenase (acid forming) or a carboxylic acid reductase, wherein 2P is an acyl-ACP reductase (alcohol forming), wherein an enzyme of the formaldehyde fixation pathway, the formate assimilation pathway, the methanol metabolic pathway, the MI-FAE cycle, the MD-FAE cycle or the termination pathway is encoded by at least one exogenous nucleic acid and is expressed in a sufficient amount to produce a compound of Formula (I):

wherein R₁ is C₁₋₂₄ linear alkyl; R₂ is CH₂OH, CHO, or COOH; R₃ is H, OH, or oxo (═O); and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four, wherein the substrate of each of said enzymes of the MI-FAE cycle, the MD-FAE cycle and the termination pathway are independently selected from a compound of Formula (II), malonyl-CoA, propionyl-CoA or acetyl-CoA:

wherein R₁ is C₁₋₂₄ linear alkyl; R₃ is H, OH, or oxo (═O); R₄ is S-CoA, ACP, OH or H; and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four; wherein said one or more enzymes of the MI-FAE cycle are each selective for a compound of Formula (II) having a number of carbon atoms at R₁ that is no greater than the number of carbon atoms at R₁ of said compound of Formula (I), wherein said one or more enzymes of the MD-FAE cycle are each selective for a compound of Formula (II) having a number of carbon atoms at R₁ that is no greater than the number of carbon atoms at R₁ of said compound of Formula (I), and wherein said one or more enzymes of the termination pathway are each selective for a compound of Formula (II) having a number of carbon atoms at R₁ that is no less than the number of carbon atoms at R₁ of said compound of Formula (I).
 2. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism has a formaldehyde fixation pathway and a MI-FAE cycle in combination with a termination pathway.
 3. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism has a formate assimilation pathway and a MI-FAE cycle in combination with a termination pathway.
 4. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, and a MI-FAE cycle in combination with a termination pathway.
 5. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism has a formaldehyde fixation pathway and a MD-FAE cycle in combination with a termination pathway.
 6. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism has a formate assimilation pathway and a MD-FAE cycle in combination with a termination pathway.
 7. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, and a MD-FAE cycle in combination with a termination pathway.
 8. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism has a methanol metabolic pathway and a MI-FAE cycle in combination with a termination pathway.
 9. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism has a methanol metabolic pathway and a MD-FAE cycle in combination with a termination pathway.
 10. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism has a formaldehyde fixation pathway, a methanol metabolic pathway and a MI-FAE cycle in combination with a termination pathway.
 11. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism has a formate assimilation pathway, a methanol metabolic pathway and a MI-FAE cycle in combination with a termination pathway.
 12. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, a methanol metabolic pathway and a MI-FAE cycle in combination with a termination pathway.
 13. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism has a formaldehyde fixation pathway, a methanol metabolic pathway and a MD-FAE cycle in combination with a termination pathway.
 14. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism has a formate assimilation pathway, a methanol metabolic pathway and a MD-FAE cycle in combination with a termination pathway.
 15. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, a methanol metabolic pathway and MD-FAE cycle in combination with a termination pathway.
 16. The non-naturally occurring microbial organism of any one of claims 1-15, wherein R₁ is C₁₋₁₇ linear alkyl.
 17. The non-naturally occurring microbial organism of claim 16, wherein R₁ is C₉ linear alkyl, C₁₀ linear alkyl, C₁₁, linear alkyl, C₁₂ linear alkyl or C₁₃ linear alkyl.
 18. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism comprises two, three, or four exogenous nucleic acids each encoding an enzyme of said MI-FAE cycle or said MD-FAE cycle.
 19. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism comprises two, three, or four exogenous nucleic acids each encoding an enzyme of said termination pathway.
 20. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism comprises one, two, three, four, five, six, seven, or eight exogenous nucleic acids each encoding a formaldehyde fixation pathway enzyme, a formate assimilation pathway enzyme, or a methanol metabolic pathway enzyme.
 21. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism comprises exogenous nucleic acids encoding each of the enzymes of at least one of the pathways selected from (1)-(49).
 22. The non-naturally occurring microbial organism of any one of claims 1-21, wherein said formate assimilation pathway further comprises: (1) 1Q; (2) 1R, and 1S; (3) 1Y and 1Q; or (4) 1Y, 1R and 1S, wherein 1Q is a pyruvate formate lyase, wherein 1R is a pyruvate dehydrogenase, a pyruvate ferredoxin oxidoreductase, or a pyruvate:NADP+ oxidoreductase, wherein 1S is a formate dehydrogenase, wherein 1Y is a glyceraldehyde-3-phosphate dehydrogenase or an enzyme of lower glycolysis.
 23. The non-naturally occurring microbial organism of any one of claims 1-22, wherein said organism further comprises a methanol oxidation pathway.
 24. The non-naturally occurring microbial organism of claim 23, wherein said organism comprises at least one exogenous nucleic acid encoding a methanol oxidation pathway enzyme expressed in a sufficient amount to produce formaldehyde in the presence of methanol, wherein said methanol oxidation pathway comprises 1A, wherein 1A a methanol dehydrogenase.
 25. The non-naturally occurring microbial organism of any one of claims 1-24, wherein said microbial organism further comprises 3H or 3P, wherein 3H is a hydrogenase, wherein 3P a carbon monoxide dehydrogenase.
 26. The non-naturally occurring micorobial organism of claim 25, wherein organism comprises an exogenous nucleic acid encoding said hydrogenase or said carbon monoxide dehydrogenase.
 27. The non-naturally occurring microbial organism of any one of claims 1-26, wherein said at least one exogenous nucleic acid encoding said formaldehyde fixation pathway enzyme, said formate assimilation pathway enzyme, said methanol metabolic pathway enzyme, said MI-FAE cycle enzyme, said MD-FAE cycle enzyme, said termination pathway enzyme, said methanol oxidation pathway enzyme, said hydrogenase or said carbon monoxide dehydrogenase is a heterologous nucleic acid.
 28. The non-naturally occurring microbial organism of any one of claims 1-27, wherein said non-naturally occurring microbial organism is in a substantially anaerobic culture medium.
 29. The non naturally occurring microbial organism of claim 1, wherein said enzyme of the formaldehyde fixation pathway, formate assimilation pathway, methanol metabolic pathway, MI-FAE cycle, MD-FAE cycle or termination pathway is expressed in a sufficient amount to produce a compound selected from the Formulas (III)-(VI):

wherein R₁ is C₁₋₁₇ linear alkyl.
 30. The non-naturally occurring microbial organism of claim 29, wherein R₁ is C₉ linear alkyl, C₁₀ linear alkyl, C₁₁, linear alkyl, C₁₂ linear alkyl or C₁₃ linear alkyl.
 31. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism further comprises an acetyl-CoA pathway and at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme expressed in a sufficient amount to produce or enhance carbon flux through acetyl-CoA, wherein said acetyl-CoA pathway comprises a pathway selected from: (1) 3A and 3B; (2) 3A, 3C, and 3D; (3) 3H; (4) 3G and 3D; (5) 3E, 3F and 3B; (6) 3E and 3I; (7) 3J, 3F and 3B; (8) 3J and 3I; (9) 4A, 4B, and 4C; (10) 4A, 4B, 4J, 4K, and 4D; (11) 4A, 4B, 4G, and 4D; (12) 4A, 4F, and 4D; (13) 4N, 4H, 4B and 4C; (14) 4N, 4H, 4B, 4J, 4K, and 4D; (15) 4N, 4H, 4B, 4G, and 4D; (16) 4N, 4H, 4F, and 4D; (17) 4L, 4M, 4B and 4C; (18) 4L, 4M, 4B, 4J, 4K, and 4D; (19) 4L, 4M, 4B, 4G, and 4D; (20) 4L, 4M, 4F, and 4D; (21) 5A, 5B, 5D, 5H, 5I, and 5J; (22) 5A, 5B, 5E, 5F, 5H, 5I, and 5J; (23) 5A, 5B, 5E, 5K, 5L, 5H, 5I, and 5J; (24) 5A, 5C, 5D, 5H, and 5J; (25) 5A, 5C, 5E, 5F, 5H, and 5J; (26) 5A, 5C, 5E, 5K, 5L, 5H, and 5J; (27) 6A, 6B, 6D, and 6G; (28) 6A, 6B, 6E, 6F, and 6G; (29) 6A, 6B, 6E, 6K, 6L, and 6G; (30) 6A, 6C, and 6D; (31) 6A, 6C, 6E, and 6F; (32) 6A, 6C, 6E, 6K, and 6L; (33) 1T and 1V; (34) 1T, 1W, and 1X; (35) 1U and 1V; and (36) 1U, 1W, and 1X, wherein 3A is a pyruvate oxidase (acetate-forming), wherein 3B is an acetyl-CoA synthetase, an acetyl-CoA ligase or an acetyl-CoA transferase, wherein 3C is an acetate kinase, wherein 3D is a phosphotransacetylase, wherein 3E is a pyruvate decarboxylase, wherein 3F is an acetaldehyde dehydrogenase, wherein 3G is a pyruvate oxidase (acetyl-phosphate forming), wherein 3H is a pyruvate dehydrogenase, a pyruvate:ferredoxin oxidoreductase, a pyruvate:NAD(P)H oxidoreductase or a pyruvate formate lyase, wherein 3I is an acetaldehyde dehydrogenase (acylating), wherein 3J is a threonine aldolase, wherein 4A is a phosphoenolpyruvate (PEP) carboxylase or a PEP carboxykinase, wherein 4B is an oxaloacetate decarboxylase, wherein 4C is a malonate semialdehyde dehydrogenase (acetylating), wherein 4D is an acetyl-CoA carboxylase or a malonyl-CoA decarboxylase, wherein 4F is an oxaloacetate dehydrogenase or an oxaloacetate oxidoreductase, wherein 4G is a malonate semialdehyde dehydrogenase (acylating), wherein 4H is a pyruvate carboxylase, wherein 4J is a malonate semialdehyde dehydrogenase, wherein 4K is a malonyl-CoA synthetase or a malonyl-CoA transferase, wherein 4L is a malic enzyme, wherein 4M is a malate dehydrogenase or a malate oxidoreductase, wherein 4N is a pyruvate kinase or a PEP phosphatase, wherein 5A is a citrate synthase, wherein 5B is a citrate transporter, wherein 5C is a citrate/malate transporter, wherein 5D is an ATP citrate lyase, wherein 5E is a citrate lyase, wherein 5F is an acetyl-CoA synthetase or an acetyl-CoA transferase, wherein 5H is a cytosolic malate dehydrogenase, wherein 5I is a malate transporter, wherein 5J is a mitochondrial malate dehydrogenase, wherein 5K is an acetate kinase, wherein 5L is a phosphotransacetylase, wherein 6A is a citrate synthase, wherein 6B is a citrate transporter, wherein 6C is a citrate/oxaloacetate transporter, wherein 6D is an ATP citrate lyase, wherein 6E is a citrate lyase, wherein 6F is an acetyl-CoA synthetase or an acetyl-CoA transferase, wherein 6G is an oxaloacetate transporter, wherein 6K is an acetate kinase, wherein 6L is a phosphotransacetylase, wherein 1T is a fructose-6-phosphate phosphoketolase, wherein 1U is a xylulose-5-phosphate phosphoketolase, wherein 1V is a phosphotransacetylase, wherein 1W is an acetate kinase, wherein 1X is an acetyl-CoA transferase, an acetyl-CoA synthetase, or an acetyl-CoA ligase.
 32. The non-naturally occurring microbial organism of claim 31, wherein said microbial organism comprises two, three, four, five, six, seven or eight exogenous nucleic acids each encoding an acetyl-CoA pathway enzyme.
 33. The non-naturally occurring microbial organism of claim 32, wherein said microbial organism comprises exogenous nucleic acids encoding each of the acetyl-CoA pathway enzymes of at least one of the pathways selected from (1)-(36).
 34. The non-naturally occurring microbial organism of claim 1, further comprising one or more gene disruptions, said one or more gene disruptions occurring in endogenous genes encoding proteins or enzymes involved in: native production of ethanol, glycerol, acetate, formate, lactate, CO₂, fatty acids, or malonyl-CoA by said microbial organism; transfer of pathway intermediates to cellular compartments other than the cytosol; or native degradation of a MI-FAE cycle intermediate, MD-FAE cycle intermediate or a termination pathway intermediate by said microbial organism, wherein said one or more gene disruptions confer increased production of the compound of Formula (I) in said microbial organism.
 35. The non-naturally occurring microbial organism of claim 34, wherein said protein or enzyme is selected from the group consisting of a fatty acid synthase, an acetyl-CoA carboxylase, a biotin:apoenzyme ligase, an acyl carrier protein, a thioesterase, an acyltransferase, an ACP malonyltransferase, a fatty acid elongase, an acyl-CoA synthetase, an acyl-CoA transferase, an acyl-CoA hydrolase, a pyruvate decarboxylase, a lactate dehydrogenase, an alcohol dehydrogenase, an acid-forming aldehyde dehydrogenases, an acetate kinase, a phosphotransacetylase, a pyruvate oxidase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate phosphatase, a mitochondrial pyruvate carrier, a peroxisomal fatty acid transporter, a peroxisomal acyl-CoA transporter, a peroxisomal carnitine/acylcarnitine transferase, an acyl-CoA oxidase, and an acyl-CoA binding protein.
 36. The non-naturally occurring microbial organism of claim 1, wherein one or more enzymes of the MI-FAE cycle, MD-FAE cycle or the termination pathway preferentially react with an NADH cofactor or have reduced preference for reacting with an NAD(P)H cofactor, wherein said one or more enzymes of the MI-FAE cycle or MD-FAE cycle are a 3-ketoacyl-CoA reductase or an enoyl-CoA reductase, and wherein said one or more enzymes of the termination pathway are selected from an acyl-CoA reductase (aldehyde forming), an alcohol dehydrogenase, an acyl-CoA reductase (alcohol forming), an aldehyde decarbonylase, an acyl-ACP reductase, an aldehyde dehydrogenase (acid forming) and a carboxylic acid reductase.
 37. The non-naturally occurring microbial organism of claim 1, further comprising one or more gene disruptions, said one or more gene disruptions occurring in genes encoding proteins or enzymes that result in an increased ratio of NAD(P)H to NAD(P) present in the cytosol of said microbial organism following said disruptions.
 38. The non-naturally occurring microbial organism of claim 37, wherein said gene encoding a protein or enzyme that results in an increased ratio of NAD(P)H to NAD(P) present in the cytosol of said microbial organism following said disruptions is selected from the group consisting of an NADH dehydrogenase, a cytochrome oxidase, a glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate phosphatase, an alcohol dehydrogenase, a pyruvate decarboxylase, an aldehyde dehydrogenase (acid forming), a lactate dehydrogenase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate:quinone oxidoreductase, a malic enzyme and a malate dehydrogenase.
 39. The non-naturally occurring organism of claim 34 or 37, wherein said one or more gene disruptions comprises a deletion of said one or more genes.
 40. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism is Crabtree positive and is in culture medium comprising excess glucose, thereby increasing the ratio of NAD(P)H to NAD(P) present in the cytosol of said microbial organism.
 41. The non-naturally occurring microbial organism of claim 1, further comprising at least one exogenous nucleic acid encoding an extracellular transporter or an extracellular transport system for the compound of Formula (I).
 42. The non-naturally occurring microbial organism of claim 1, wherein one or more endogenous enzymes involved in: native production of ethanol, glycerol, acetate, formate, lactate, CO2, fatty acids, or malonyl-CoA by said microbial organism; transfer of pathway intermediates to cellular compartments other than the cytosol; or native degradation of a MI-FAE cycle intermediate, MD-FAE cycle intermediate or a termination pathway intermediate by said microbial organism, has attenuated enzyme activity or expression levels.
 43. The non-naturally occurring microbial organism of claim 42, wherein said enzyme is selected from the group consisting of a fatty acid synthase, an acetyl-CoA carboxylase, a biotin:apoenzyme ligase, a thioesterase, an acyl carrier protein, a thioesterase, an acyltransferase, an ACP malonyltransferase, a fatty acid elongase, an acyl-CoA synthetase, an acyl-CoA transferase, an acyl-CoA hydrolase, a pyruvate decarboxylase, a lactate dehydrogenase, a short-chain alcohol dehydrogenase, an acid-forming aldehyde dehydrogenase, an acetate kinase, a phosphotransacetylase, a pyruvate oxidase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate phosphatase, a mitochondrial pyruvate carrier, a peroxisomal fatty acid transporter, a peroxisomal acyl-CoA transporter, a peroxisomal carnitine/acylcarnitine transferase, an acyl-CoA oxidase, and an acyl-CoA binding protein.
 44. The non-naturally occurring microbial organism of claim 1, wherein one or more endogenous enzymes involved in the oxidation of NAD(P)H or NADH, has attenuated enzyme activity or expression levels.
 45. The non-naturally occurring microbial organism of claim 44, wherein said one or more endogenous enzymes are selected from the group consisting of an NADH dehydrogenase, a cytochrome oxidase, a glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate phosphatase, an alcohol dehydrogenase, a pyruvate decarboxylase, an aldehyde dehydrogenase (acid forming), a lactate dehydrogenase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate:quinone oxidoreductase, a malic enzyme and a malate dehydrogenase.
 46. A method for producing a compound of Formula (I):

wherein R₁ is C₁₋₂₄ linear alkyl; R₂ is CH₂OH, CHO, or COOH; R₃ is H, OH, or oxo (═O); and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four, comprising culturing the non-naturally occurring microbial organism of any one of claims 1-45 under conditions for a sufficient period of time to produce said compound of Formula (I).
 47. The method of claim 46, wherein said method further comprises separating the compound of Formula (I) from other components in the culture.
 48. The method of claim 47, wherein the separating comprises extraction, continuous liquid-liquid extraction, pervaporation, membrane filtration, membrane separation, reverse osmosis, electrodialysis, distillation, crystallization, centrifugation, extractive filtration, ion exchange chromatography, absorption chromatography, or ultrafiltration.
 49. Culture medium comprising bioderived compound of Formula (I):

wherein R₁ is C₁₋₂₄ linear alkyl; R₂ is CH₂OH, CHO, or COOH; R₃ is H, OH, or oxo (═O); and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four, wherein said culture medium is separated from a non-naturally occurring microbial organism of any one of claims 1-45.
 50. A bioderived compound of Formula (I):

wherein R₁ is C₁₋₂₄ linear alkyl; R₂ is CH₂OH, CHO, or COOH; R₃ is H, OH, or oxo (═O); and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four, wherein said bioderived compound is produced according to the method of any one of claims 46-48.
 51. The bioderived compound of claim 50, wherein said bioderived compound has an Fm value of at least 80%, at least 85%, at least 90%, at least 95% or at least 98%.
 52. A composition comprising said bioderived compound of claim 50 or 51 and a compound other than said bioderived compound.
 53. The composition of claim 52 wherein said compound other than said bioderived compound is a trace amount of a cellular portion of a non-naturally occurring microbial organism having: (i) a formaldehyde fixation pathway; (ii) a formate assimilation pathway; and/or (iii) a methanol metabolic pathway, and a malonyl-CoA independent fatty acyl-CoA elongation (MI-FAE) cycle and/or a malonyl-CoA dependent fatty acyl-CoA elongation (MD-FAE) cycle in combination with a termination pathway.
 54. A composition comprising the bioderived compound of claim 50 or 51, or a cell lysate or culture supernatant thereof.
 55. A biobased product comprising said bioderived compound of claim 50 or 51, wherein said biobased product is a biofuel, chemical, polymer, surfactant, soap, detergent, shampoo, lubricating oil additive, fragrance, flavor material or acrylate.
 56. The biobased product of claim 55 comprising at least 5%, at least 10%, at least 20%, at least 30%, at least 40% or at least 50% said bioderived compound.
 57. The biobased product of claim 55 or 56, wherein said biobased product comprises a portion of said bioderived compound as a repeating unit.
 58. A molded product obtained by molding a biobased product of any one of claims 55-57, wherein said biobased product is a polymer.
 59. A process for producing a biobased product of any one of claims 55-57 comprising chemically reacting said bioderived compound with itself or another compound in a reaction that produces said biobased product.
 60. A non-naturally occurring microbial organism having: (i) a formaldehyde fixation pathway; (ii) a formate assimilation pathway; and/or (iii) a methanol metabolic pathway, and an isopropanol pathway, wherein said formaldehyde fixation pathway comprises: (1) 1B and 1C; (2) 1D; (3) 1D and 1Z, wherein 1B is a 3-hexulose-6-phosphate synthase, wherein 1C is a 6-phospho-3-hexuloisomerase, wherein 1D is a dihydroxyacetone synthase, wherein 1Z is a fructose-6-phosphate aldolase, wherein said formate assimilation pathway comprises a pathway selected from: (4) 1E; (5) 1F, and 1G; (6) 1H, 1I, 1J, and 1K; (7) 1H, 1I, 1J, 1L, 1M, and 1N; (8) 1E, 1H, 1I, 1J, 1L, 1M, and 1N; (9) 1F, 1G, 1H, 1I, 1J, 1L, 1M, and 1N; (10) 1K, 1H, 1I, 1J, 1L, 1M, and 1N; and (11) 1H, 1I, 1J, 1O, and 1P, wherein 1E is a formate reductase, 1F is a formate ligase, a formate transferase, or a formate synthetase, wherein 1G is a formyl-CoA reductase, wherein 1H is a formyltetrahydrofolate synthetase, wherein 1I is a methenyltetrahydrofolate cyclohydrolase, wherein 1J is a methylenetetrahydrofolate dehydrogenase, wherein 1K is a formaldehyde-forming enzyme or spontaneous, wherein 1L is a glycine cleavage system, wherein 1M is a serine hydroxymethyltransferase, wherein 1N is a serine deaminase, wherein 1O is a methylenetetrahydrofolate reductase, wherein 1P is an acetyl-CoA synthase, wherein said methanol metabolic pathway comprises a pathway selected from: (12) 10J; (13) 10A; (14) 10A and 10B; (15) 10A, 10B and 10C; (16) 10J, 10K and 10C; (17) 10J, 10M, and 10N; (18) 10J and 10L; (19) 10J, 10L, and 10G; (20) 10J, 10L, and 10I; (21) 10A, 10B, 10C, 10D, and 10E; (22) 10A, 10B, 10C, 10D, and 10F; (23) 10J, 10K, 10C, 10D, and 10E; (24) 10J, 10K, 10C, 10D, and 10F; (25) 10J, 10M, 10N, and 10O; (26) 10A, 10B, 10C, 10D, 10E, and 10G; (27) 10A, 10B, 10C, 10D, 10F, and 10G; (28) 10J, 10K, 10C, 10D, 10E, and 10G; (29) 10J, 10K, 10C, 10D, 10F, and 10G; (30) 10J, 10M, 10N, 10O, and 10G; (31) 10A, 10B, 10C, 10D, 10E, and 10I; (32) 10A, 10B, 10C, 10D, 10F, and 10I; (33) 10J, 10K, 10C, 10D, 10E, and 10I; (34) 10J, 10K, 10C, 10D, 10F, and 10I; and (35) 10J, 10M, 10N, 10O, and 10I, wherein 10A is a methanol methyltransferase, wherein 10B is a methylenetetrahydrofolate reductase, wherein 10C is a methylenetetrahydrofolate dehydrogenase, wherein 10D is a methenyltetrahydrofolate cyclohydrolase, wherein 10E is a formyltetrahydrofolate deformylase, wherein 10F is a formyltetrahydrofolate synthetase, wherein 10G is a formate hydrogen lyase, wherein 10I is a formate dehydrogenase, wherein 10J is a methanol dehydrogenase, wherein 10K is a formaldehyde activating enzyme or spontaneous, wherein 10L is a formaldehyde dehydrogenase, wherein 10M is a S-(hydroxymethyl)glutathione synthase or spontaneous, wherein 10N is a glutathione-dependent formaldehyde dehydrogenase, wherein 10O is a S-formylglutathione hydrolase, wherein said isopanol pathway comprises: (36) 11V, 11W, 11X, and 11Y; or (37) 11T, 11U, 11W, 11X, and 11Y, wherein 11T is an acetyl-CoA carboxylase, wherein 11U is an acetoacetyl-CoA synthase, wherein 11V is an acetyl-CoA:acetyl-CoA acyltransferase, wherein 11W is an acetoacetyl-CoA hydrolase, an acetoacetyl-CoA transferase, an acetoacetyl-CoA ligase, or a phosphotransacetoacetylase/acetoacetate kinase, wherein 11X is an acetoacetate decarboxylase, wherein 11Y is an acetone reductase or isopropanol dehydrogenase, wherein an enzyme of the formaldehyde fixation pathway, formate assimilation pathway, methanol metabolic pathway, or isopropanol pathway is encoded by at least one exogenous nucleic acid and is expressed in a sufficient amount to produce isopropanol.
 61. The non-naturally occurring microbial organism of claim 60, wherein said microbial organism has a formaldehyde fixation pathway and an isopropanol pathway.
 62. The non-naturally occurring microbial organism of claim 60, wherein said microbial organism has a formate assimilation pathway and an isopropanol pathway.
 63. The non-naturally occurring microbial organism of claim 60, wherein said microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, and an isopropanol pathway.
 64. The non-naturally occurring microbial organism of claim 60, wherein said microbial organism has a methanol metabolic pathway and an isopropanol pathway.
 65. The non-naturally occurring microbial organism of claim 60, wherein said microbial organism has a formaldehyde fixation pathway, a methanol metabolic pathway and an isopropanol pathway.
 66. The non-naturally occurring microbial organism of claim 60, wherein said microbial organism has a formate assimilation pathway, a methanol metabolic pathway and an isopropanol pathway.
 67. The non-naturally occurring microbial organism of claim 60, wherein said microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, a methanol metabolic pathway and an isopropanol pathway.
 68. The non-naturally occurring microbial organism of claim 60, wherein said microbial organism comprises two, three, four, five or six exogenous nucleic acids each encoding an enzyme of said isopropanol pathway.
 69. The non-naturally occurring microbial organism of claim 60, wherein said microbial organism comprises one, two, three, four, five, six, seven, or eight exogenous nucleic acids each encoding a formaldehyde fixation pathway enzyme, a formate assimilation pathway enzyme, or a methanol metabolic pathway enzyme.
 70. The non-naturally occurring microbial organism of claim 60, wherein said microbial organism comprises exogenous nucleic acids encoding each of the enzymes of at least one of the pathways selected from (1)-(37).
 71. The non-naturally occurring microbial organism of any one of claims 60-70, wherein said formate assimilation pathway further comprises: (1) 1Q; (2) 1R, and 1S; (3) 1Y and 1Q; or (4) 1Y, 1R and 1S, wherein 1Q is a pyruvate formate lyase, wherein 1R is a pyruvate dehydrogenase, a pyruvate ferredoxin oxidoreductase, or a pyruvate:NADP+ oxidoreductase, wherein 1S is a formate dehydrogenase, wherein 1Y is a glyceraldehyde-3-phosphate dehydrogenase or an enzyme of lower glycolysis.
 72. The non-naturally occurring microbial organism of any one of claims 60-71, wherein said organism further comprises a methanol oxidation pathway.
 73. The non-naturally occurring microbial organism of claim 72, wherein said organism comprises at least one exogenous nucleic acid encoding a methanol oxidation pathway enzyme expressed in a sufficient amount to produce formaldehyde in the presence of methanol, wherein said methanol oxidation pathway comprises 1A, wherein 1A a methanol dehydrogenase.
 74. The non-naturally occurring microbial organism of any one of claims 60-73, wherein said microbial organism further comprises 3H or 3P, wherein 3H is a hydrogenase, wherein 3P a carbon monoxide dehydrogenase.
 75. The non-naturally occurring micorobial organism of claim 74, wherein organism comprises an exogenous nucleic acid encoding said hydrogenase or said carbon monoxide dehydrogenase.
 76. The non-naturally occurring microbial organism of any one of claims 60-75, wherein said at least one exogenous nucleic acid encoding said formaldehyde fixation pathway enzyme, said formate assimilation pathway enzyme, said methanol metabolic pathway enzyme, said isopropanol pathway, said methanol oxidation pathway enzyme, said hydrogenase or said carbon monoxide dehydrogenase is a heterologous nucleic acid.
 77. The non-naturally occurring microbial organism of any one of claims 60-76, wherein said non-naturally occurring microbial organism is in a substantially anaerobic culture medium.
 78. The non-naturally occurring microbial organism of claim 60, wherein said microbial organism further comprises an acetyl-CoA pathway and at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme expressed in a sufficient amount to produce or enhance carbon flux through acetyl-CoA, wherein said acetyl-CoA pathway comprises a pathway selected from: (1) 3A and 3B; (2) 3A, 3C, and 3D; (3) 3H; (4) 3G and 3D; (5) 3E, 3F and 3B; (6) 3E and 3I; (7) 3J, 3F and 3B; (8) 3J and 3I; (9) 4A, 4B, and 4C; (10) 4A, 4B, 4J, 4K, and 4D; (11) 4A, 4B, 4G, and 4D; (12) 4A, 4F, and 4D; (13) 4N, 4H, 4B and 4C; (14) 4N, 4H, 4B, 4J, 4K, and 4D; (15) 4N, 4H, 4B, 4G, and 4D; (16) 4N, 4H, 4F, and 4D; (17) 4L, 4M, 4B and 4C; (18) 4L, 4M, 4B, 4J, 4K, and 4D; (19) 4L, 4M, 4B, 4G, and 4D; (20) 4L, 4M, 4F, and 4D; (21) 5A, 5B, 5D, 5H, 5I, and 5J; (22) 5A, 5B, 5E, 5F, 5H, 5I, and 5J; (23) 5A, 5B, 5E, 5K, 5L, 5H, 5I, and 5J; (24) 5A, 5C, 5D, 5H, and 5J; (25) 5A, 5C, 5E, 5F, 5H, and 5J; (26) 5A, 5C, 5E, 5K, 5L, 5H, and 5J; (27) 6A, 6B, 6D, and 6G; (28) 6A, 6B, 6E, 6F, and 6G; (29) 6A, 6B, 6E, 6K, 6L, and 6G; (30) 6A, 6C, and 6D; (31) 6A, 6C, 6E, and 6F; (32) 6A, 6C, 6E, 6K, and 6L; (33) 1T and 1V; (34) 1T, 1W, and 1X; (35) 1U and 1V; and (36) 1U, 1W, and 1X, wherein 3A is a pyruvate oxidase (acetate-forming), wherein 3B is an acetyl-CoA synthetase, an acetyl-CoA ligase or an acetyl-CoA transferase, wherein 3C is an acetate kinase, wherein 3D is a phosphotransacetylase, wherein 3E is a pyruvate decarboxylase, wherein 3F is an acetaldehyde dehydrogenase, wherein 3G is a pyruvate oxidase (acetyl-phosphate forming), wherein 3H is a pyruvate dehydrogenase, a pyruvate:ferredoxin oxidoreductase, a pyruvate:NAD(P)H oxidoreductase or a pyruvate formate lyase, wherein 3I is an acetaldehyde dehydrogenase (acylating), wherein 3J is a threonine aldolase, wherein 4A is a phosphoenolpyruvate (PEP) carboxylase or a PEP carboxykinase, wherein 4B is an oxaloacetate decarboxylase, wherein 4C is a malonate semialdehyde dehydrogenase (acetylating), wherein 4D is an acetyl-CoA carboxylase or a malonyl-CoA decarboxylase, wherein 4F is an oxaloacetate dehydrogenase or an oxaloacetate oxidoreductase, wherein 4G is a malonate semialdehyde dehydrogenase (acylating), wherein 4H is a pyruvate carboxylase, wherein 4J is a malonate semialdehyde dehydrogenase, wherein 4K is a malonyl-CoA synthetase or a malonyl-CoA transferase, wherein 4L is a malic enzyme, wherein 4M is a malate dehydrogenase or a malate oxidoreductase, wherein 4N is a pyruvate kinase or a PEP phosphatase, wherein 5A is a citrate synthase, wherein 5B is a citrate transporter, wherein 5C is a citrate/malate transporter, wherein 5D is an ATP citrate lyase, wherein 5E is a citrate lyase, wherein 5F is an acetyl-CoA synthetase or an acetyl-CoA transferase, wherein 5H is a cytosolic malate dehydrogenase, wherein 51 is a malate transporter, wherein 5J is a mitochondrial malate dehydrogenase, wherein 5K is an acetate kinase, wherein 5L is a phosphotransacetylase, wherein 6A is a citrate synthase, wherein 6B is a citrate transporter, wherein 6C is a citrate/oxaloacetate transporter, wherein 6D is an ATP citrate lyase, wherein 6E is a citrate lyase, wherein 6F is an acetyl-CoA synthetase or an acetyl-CoA transferase, wherein 6G is an oxaloacetate transporter, wherein 6K is an acetate kinase, and wherein 6L is a phosphotransacetylase, wherein 1T is a fructose-6-phosphate phosphoketolase, wherein 1U is a xylulose-5-phosphate phosphoketolase, wherein 1V is a phosphotransacetylase, wherein 1W is an acetate kinase, wherein 1X is an acetyl-CoA transferase, an acetyl-CoA synthetase, or an acetyl-CoA ligase.
 79. The non-naturally occurring microbial organism of claim 78, wherein said microbial organism comprises two, three, four, five, six, seven or eight exogenous nucleic acids each encoding an acetyl-CoA pathway enzyme.
 80. The non-naturally occurring microbial organism of claim 79, wherein said microbial organism comprises exogenous nucleic acids encoding each of the acetyl-CoA pathway enzymes of at least one of the pathways selected from (1)-(36).
 81. A method for producing isopropanol comprising culturing the non-naturally occurring microbial organism of any one of claims 60-80 under conditions for a sufficient period of time to produce isopropanol.
 82. The method of claim 81, wherein said method further comprises separating the isopropanol from other components in the culture.
 83. The method of claim 82, wherein the separating comprises extraction, continuous liquid-liquid extraction, pervaporation, membrane filtration, membrane separation, reverse osmosis, electrodialysis, distillation, crystallization, centrifugation, extractive filtration, ion exchange chromatography, absorption chromatography, or ultrafiltration.
 84. Culture medium comprising bioderived isopropanol, wherein said culture medium is separated from a non-naturally occurring microbial organism of any one of claims 60-80.
 85. A bioderived isopropanol produced according to the method of any one of claims 81-83.
 86. The bioderived isopropanol of claim 85, wherein said bioderived compound has an Fm value of at least 80%, at least 85%, at least 90%, at least 95% or at least 98%.
 87. A composition comprising said bioderived isopropanol of claim 85 or 86 and a compound other than said bioderived isopropanol.
 88. The composition of claim 87 wherein said compound other than said bioderived compound is a trace amount of a cellular portion of a non-naturally occurring microbial organism having: (i) a formaldehyde fixation pathway; (ii) a formate assimilation pathway; and/or (iii) a methanol metabolic pathway, and an isopropanol pathway.
 89. A composition comprising the bioderived isopropanol of claim 85 or 86, or a cell lysate or culture supernatant thereof.
 90. A biobased product comprising said bioderived compound of claim 85 or 86, wherein said biobased product is a solvent, a paint, lacquer, thinner, ink, adhesive, cleaner, disinfectant, cosmetic, toiletry, de-icer, pharmaceutical, motor oil, isopropylamine, isopropylether, isopropyl ester, propylene or a polymer.
 91. The biobased product of claim 90 comprising at least 5%, at least 10%, at least 20%, at least 30%, at least 40% or at least 50% said bioderived compound.
 92. The biobased product of claim 90 or 91, wherein said biobased product comprises a portion of said bioderived compound as a repeating unit.
 93. A molded product obtained by molding a biobased product of any one of claims 90-92, wherein said biobased product is a polymer.
 94. A process for producing a biobased product of any one of claims 90-92 comprising chemically reacting said bioderived compound with itself or another compound in a reaction that produces said biobased product.
 95. A non-naturally occurring microbial organism having: (i) a formaldehyde fixation pathway; (ii) a formate assimilation pathway; and/or (iii) a methanol metabolic pathway, and a fatty acyl-ACP elongation (FAACPE) cycle in combination with a termination pathway, wherein said formaldehyde fixation pathway comprises: (1) 1B and 1C; (2) 1D; or (3) 1D and 1Z, wherein 1B is a 3-hexulose-6-phosphate synthase, wherein 1C is a 6-phospho-3-hexuloisomerase, wherein 1D is a dihydroxyacetone synthase, wherein 1Z is a fructose-6-phosphate aldolase, wherein said formate assimilation pathway comprises a pathway selected from: (4) 1E; (5) 1F, and 1G; (6) 1H, 1I, 1J, and 1K; (7) 1H, 1I, 1J, 1L, 1M, and 1N; (8) 1E, 1H, 1I, 1J, 1L, 1M, and 1N; (9) 1F, 1G, 1H, 1I, 1J, 1L, 1M, and 1N; (10) 1K, 1H, 1I, 1J, 1L, 1M, and 1N; and (11) 1H, 1I, 1J, 1O, and 1P, wherein 1E is a formate reductase, 1F is a formate ligase, a formate transferase, or a formate synthetase, wherein 1G is a formyl-CoA reductase, wherein 1H is a formyltetrahydrofolate synthetase, wherein 1I is a methenyltetrahydrofolate cyclohydrolase, wherein 1J is a methylenetetrahydrofolate dehydrogenase, wherein 1K is a formaldehyde-forming enzyme or spontaneous, wherein 1L is a glycine cleavage system, wherein 1M is a serine hydroxymethyltransferase, wherein 1N is a serine deaminase, wherein 1O is a methylenetetrahydrofolate reductase, wherein 1P is an acetyl-CoA synthase, wherein said methanol metabolic pathway comprises a pathway selected from: (12) 10J; (13) 10A; (14) 10A and 10B; (15) 10A, 10B and 10C; (16) 10J, 10K and 10C; (17) 10J, 10M, and 10N; (18) 10J and 10L; (19) 10J, 10L, and 10G; (20) 10J, 10L, and 10I; (21) 10A, 10B, 10C, 10D, and 10E; (22) 10A, 10B, 10C, 10D, and 10F; (23) 10J, 10K, 10C, 10D, and 10E; (24) 10J, 10K, 10C, 10D, and 10F; (25) 10J, 10M, 10N, and 10O; (26) 10A, 10B, 10C, 10D, 10E, and 10G; (27) 10A, 10B, 10C, 10D, 10F, and 10G; (28) 10J, 10K, 10C, 10D, 10E, and 10G; (29) 10J, 10K, 10C, 10D, 10F, and 10G; (30) 10J, 10M, 10N, 10O, and 10G; (31) 10A, 10B, 10C, 10D, 10E, and 10I; (32) 10A, 10B, 10C, 10D, 10F, and 10I; (33) 10J, 10K, 10C, 10D, 10E, and 10I; (34) 10J, 10K, 10C, 10D, 10F, and 10I; and (35) 10J, 10M, 10N, 10O, and 10I, wherein 10A is a methanol methyltransferase, wherein 10B is a methylenetetrahydrofolate reductase, wherein 10C is a methylenetetrahydrofolate dehydrogenase, wherein 10D is a methenyltetrahydrofolate cyclohydrolase, wherein 10E is a formyltetrahydrofolate deformylase, wherein 10F is a formyltetrahydrofolate synthetase, wherein 10G is a formate hydrogen lyase, wherein 10I is a formate dehydrogenase, wherein 10J is a methanol dehydrogenase, wherein 10K is a formaldehyde activating enzyme or spontaneous, wherein 10L is a formaldehyde dehydrogenase, wherein 10M is a S-(hydroxymethyl)glutathione synthase or spontaneous, wherein 10N is a glutathione-dependent formaldehyde dehydrogenase, wherein 10O is a S-formylglutathione hydrolase, wherein said FAACPE cycle comprises one or more β-ketoacyl-ACP synthase, one or more β-ketoacyl-ACP reductase, one or more β-hydroxyacyl-ACP reductase, and one or more enoyl ACP-reductase, wherein said termination pathway comprises a pathway selected from: (36) 12I; (37) 12J; (38) 12I, 12K, and 12L; (39) 12I and 12O; (40) 12J and 12M; (41) 12I, 12K, 12L, and 12M; (42) 12I, 12O, and 12M; (43) 12I, 12K and 12N; and (44) 12P, wherein 12I is a thioesterase, wherein 12J is a fatty acyl-ACP reductase, wherein 12K is an acyl-CoA synthase, wherein 12L is an acyl-CoA reductase, wherein 12M is a fatty aldehyde reductase, wherein 12N is a fatty alcohol forming acyl-CoA reductase (FAR), wherein 12O is a carboxylic acid reductase (CAR), wherein 12P is an acyl-ACP reductase (alcohol forming), wherein an enzyme of the formaldehyde fixation pathway, the formate assimilation pathway, the methanol metabolic pathway, the FAACPE cycle or the termination pathway is encoded by at least one exogenous nucleic acid and is expressed in a sufficient amount to produce a compound of Formula (I):

wherein R₁ is C₁₋₂₄ linear alkyl; R₂ is CH₂OH, CHO, or COOH; R₃ is H, OH, or oxo (═O); and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four, wherein the substrate of each of said enzymes of the FAACPE cycle and the termination pathway are independently selected from a compound of Formula (II) or malonyl-ACP:

wherein R₁ is C₁₋₂₄ linear alkyl; R₃ is H, OH, or oxo (═O); R₄ is S-CoA, ACP, OH or H; and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four; wherein said one or more enzymes of the FAACPE cycle are each selective for a compound of Formula (II) having a number of carbon atoms at R₁ that is no greater than the number of carbon atoms at R₁ of said compound of Formula (I), and wherein said one or more enzymes of the termination pathway are each selective for a compound of Formula (II) having a number of carbon atoms at R₁ that is no less than the number of carbon atoms at R₁ of said compound of Formula (I).
 96. The non-naturally occurring microbial organism of claim 95, wherein said microbial organism has a formaldehyde fixation pathway and an FAACPE cycle in combination with a termination pathway.
 97. The non-naturally occurring microbial organism of claim 95, wherein said microbial organism has a formate assimilation pathway and an FAACPE cycle in combination with a termination pathway.
 98. The non-naturally occurring microbial organism of claim 95, wherein said microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, and an FAACPE cycle in combination with a termination pathway.
 99. The non-naturally occurring microbial organism of claim 95, wherein said microbial organism has a methanol metabolic pathway and an FAACPE cycle in combination with a termination pathway.
 100. The non-naturally occurring microbial organism of claim 95, wherein said microbial organism has a formaldehyde fixation pathway, a methanol metabolic pathway and an FAACPE cycle in combination with a termination pathway.
 101. The non-naturally occurring microbial organism of claim 95, wherein said microbial organism has a formate assimilation pathway, a methanol metabolic pathway and an FAACPE cycle in combination with a termination pathway.
 102. The non-naturally occurring microbial organism of claim 95, wherein said microbial organism has a formaldehyde fixation pathway, a formate assimilation pathway, a methanol metabolic pathway and an FAACPE cycle in combination with a termination pathway.
 103. The non-naturally occurring microbial organism of any one of claims 95-102, wherein the microbial organism further comprises an acetoacetyl-ACP pathway of (1) 12A, 12B, and 12C; or (2) 12A, 12B, and 12D, wherein 12A is an acetyl-CoA carboxylase, wherein 12B is malonyl-CoA ACP transacylase, wherein 12C is an acetoacetyl-ACP synthase, and wherein 12D is a β-ketoacyl-ACP synthase, or wherein the microbial organism further comprises a 3-oxovalery-ACP pathway comprising an acetyl-CoA carboxylase, a malonyl-CoA ACP transacylase, and a β-ketoacyl-ACP synthase.
 104. The non-naturally occurring microbial organism of 103, wherein an enzyme of the acetoacetyl-ACP pathway is encoded by at least one exogenous nucleic acid and is expressed in a sufficient amount to produce acetoacetyl-ACP, wherein an enzyme of the 3-oxovalery-ACP pathway is encoded by at least one exogenous nucleic acid and is expressed in a sufficient amount to produce 3-oxovalery-ACP, and wherein the acetoacetyl-ACP or the 3-oxovalery-ACP is a β-ketoacyl-ACP of the FAACPE cycle.
 105. The non-naturally occurring microbial organism of any one of claims 95-104, wherein R₁ is C₁₋₁₇ linear alkyl.
 106. The non-naturally occurring microbial organism of claim 105, wherein R₁ is C₉ linear alkyl, C₁₀ linear alkyl, C₁₁, linear alkyl, C₁₂ linear alkyl or C₁₃ linear alkyl.
 107. The non-naturally occurring microbial organism of claim 95, wherein said microbial organism comprises two, three, or four exogenous nucleic acids each encoding an enzyme of said FAACPE cycle.
 108. The non-naturally occurring microbial organism of claim 95, wherein said microbial organism comprises two, three, or four exogenous nucleic acids each encoding an enzyme of said termination pathway.
 109. The non-naturally occurring microbial organism of claim 95, wherein said microbial organism comprises one, two, three, four, five, six, seven, or eight exogenous nucleic acids each encoding a formaldehyde fixation pathway enzyme, a formate assimilation pathway enzyme, or a methanol metabolic pathway enzyme.
 110. The non-naturally occurring microbial organism of claim 95, wherein said microbial organism comprises exogenous nucleic acids encoding each of the enzymes of at least one of the pathways selected from (1)-(44).
 111. The non-naturally occurring microbial organism of any one of claims 95-110, wherein said formate assimilation pathway further comprises: (1) 1Q; (2) 1R, and 1S; (3) 1Y and 1Q; (4) 1Y, 1R and 1S, wherein 1Q is a pyruvate formate lyase, wherein 1R is a pyruvate dehydrogenase, a pyruvate ferredoxin oxidoreductase, or a pyruvate:NADP+ oxidoreductase, wherein 1S is a formate dehydrogenase, wherein 1Y is a glyceraldehyde-3-phosphate dehydrogenase or an enzyme of lower glycolysis.
 112. The non-naturally occurring microbial organism of any one of claims 95-111, wherein said organism further comprises a methanol oxidation pathway.
 113. The non-naturally occurring microbial organism of claim 112, wherein said organism comprises at least one exogenous nucleic acid encoding a methanol oxidation pathway enzyme expressed in a sufficient amount to produce formaldehyde in the presence of methanol, wherein said methanol oxidation pathway comprises 1A, wherein 1A a methanol dehydrogenase.
 114. The non-naturally occurring microbial organism of any one of claims 95-113, wherein said microbial organism further comprises 3H or 3P, wherein 3H is a hydrogenase, wherein 3P a carbon monoxide dehydrogenase.
 115. The non-naturally occurring micorobial organism of claim 114, wherein organism comprises an exogenous nucleic acid encoding said hydrogenase or said carbon monoxide dehydrogenase.
 116. The non-naturally occurring microbial organism of any one of claims 95-115, wherein said at least one exogenous nucleic acid encoding said formaldehyde fixation pathway enzyme, said formate assimilation pathway enzyme, said methanol metabolic pathway enzyme, said FAACPE cycle enzyme, said termination pathway enzyme, said acetoacetyl-ACP pathway enzyme, said 3-oxovalery-ACP pathway enzyme, said methanol oxidation pathway enzyme, said hydrogenase or said carbon monoxide dehydrogenase is a heterologous nucleic acid.
 117. The non-naturally occurring microbial organism of any one of claims 95-116, wherein said non-naturally occurring microbial organism is in a substantially anaerobic culture medium.
 118. The non naturally occurring microbial organism of claim 95, wherein said enzyme of the formaldehyde fixation pathway, formate assimilation pathway, methanol metabolic pathway, FAACPE cycle or termination pathway is expressed in a sufficient amount to produce a compound selected from the Formulas (III)-(VI):

wherein R₁ is C₁₋₁₇ linear alkyl.
 119. The non-naturally occurring microbial organism of claim 118, wherein R₁ is C₉ linear alkyl, C₁₀ linear alkyl, C₁₁, linear alkyl, C₁₂ linear alkyl or C₁₃ linear alkyl.
 120. The non-naturally occurring microbial organism of claim 95, wherein said microbial organism further comprises an acetyl-CoA pathway and at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme expressed in a sufficient amount to produce or enhance carbon flux through acetyl-CoA, wherein said acetyl-CoA pathway comprises a pathway selected from: (1) 3A and 3B; (2) 3A, 3C, and 3D; (3) 3H; (4) 3G and 3D; (5) 3E, 3F and 3B; (6) 3E and 3I; (7) 3J, 3F and 3B; (8) 3J and 3I; (9) 4A, 4B, and 4C; (10) 4A, 4B, 4J, 4K, and 4D; (11) 4A, 4B, 4G, and 4D; (12) 4A, 4F, and 4D; (13) 4N, 4H, 4B and 4C; (14) 4N, 4H, 4B, 4J, 4K, and 4D; (15) 4N, 4H, 4B, 4G, and 4D; (16) 4N, 4H, 4F, and 4D; (17) 4L, 4M, 4B and 4C; (18) 4L, 4M, 4B, 4J, 4K, and 4D; (19) 4L, 4M, 4B, 4G, and 4D; (20) 4L, 4M, 4F, and 4D; (21) 5A, 5B, 5D, 5H, 5I, and 5J; (22) 5A, 5B, 5E, 5F, 5H, 5I, and 5J; (23) 5A, 5B, 5E, 5K, 5L, 5H, 5I, and 5J; (24) 5A, 5C, 5D, 5H, and 5J; (25) 5A, 5C, 5E, 5F, 5H, and 5J; (26) 5A, 5C, 5E, 5K, 5L, 5H, and 5J; (27) 6A, 6B, 6D, and 6G; (28) 6A, 6B, 6E, 6F, and 6G; (29) 6A, 6B, 6E, 6K, 6L, and 6G; (30) 6A, 6C, and 6D; (31) 6A, 6C, 6E, and 6F; (32) 6A, 6C, 6E, 6K, and 6L; (33) 1T and 1V; (34) 1T, 1W, and 1X; (35) 1U and 1V; and (36) 1U, 1W, and 1X, wherein 3A is a pyruvate oxidase (acetate-forming), wherein 3B is an acetyl-CoA synthetase, an acetyl-CoA ligase or an acetyl-CoA transferase, wherein 3C is an acetate kinase, wherein 3D is a phosphotransacetylase, wherein 3E is a pyruvate decarboxylase, wherein 3F is an acetaldehyde dehydrogenase, wherein 3G is a pyruvate oxidase (acetyl-phosphate forming), wherein 3H is a pyruvate dehydrogenase, a pyruvate:ferredoxin oxidoreductase, a pyruvate:NAD(P)H oxidoreductase or a pyruvate formate lyase, wherein 3I is an acetaldehyde dehydrogenase (acylating), wherein 3J is a threonine aldolase, wherein 4A is a phosphoenolpyruvate (PEP) carboxylase or a PEP carboxykinase, wherein 4B is an oxaloacetate decarboxylase, wherein 4C is a malonate semialdehyde dehydrogenase (acetylating), wherein 4D is an acetyl-CoA carboxylase or a malonyl-CoA decarboxylase, wherein 4F is an oxaloacetate dehydrogenase or an oxaloacetate oxidoreductase, wherein 4G is a malonate semialdehyde dehydrogenase (acylating), wherein 4H is a pyruvate carboxylase, wherein 4J is a malonate semialdehyde dehydrogenase, wherein 4K is a malonyl-CoA synthetase or a malonyl-CoA transferase, wherein 4L is a malic enzyme, wherein 4M is a malate dehydrogenase or a malate oxidoreductase, wherein 4N is a pyruvate kinase or a PEP phosphatase, wherein 5A is a citrate synthase, wherein 5B is a citrate transporter, wherein 5C is a citrate/malate transporter, wherein 5D is an ATP citrate lyase, wherein 5E is a citrate lyase, wherein 5F is an acetyl-CoA synthetase or an acetyl-CoA transferase, wherein 5H is a cytosolic malate dehydrogenase, wherein 5I is a malate transporter, wherein 5J is a mitochondrial malate dehydrogenase, wherein 5K is an acetate kinase, wherein 5L is a phosphotransacetylase, wherein 6A is a citrate synthase, wherein 6B is a citrate transporter, wherein 6C is a citrate/oxaloacetate transporter, wherein 6D is an ATP citrate lyase, wherein 6E is a citrate lyase, wherein 6F is an acetyl-CoA synthetase or an acetyl-CoA transferase, wherein 6G is an oxaloacetate transporter, wherein 6K is an acetate kinase, and wherein 6L is a phosphotransacetylase, wherein 1T is a fructose-6-phosphate phosphoketolase, wherein 1U is a xylulose-5-phosphate phosphoketolase, wherein 1V is a phosphotransacetylase, wherein 1W is an acetate kinase, wherein 1X is an acetyl-CoA transferase, an acetyl-CoA synthetase, or an acetyl-CoA ligase.
 121. The non-naturally occurring microbial organism of claim 120, wherein said microbial organism comprises two, three, four, five, six, seven or eight exogenous nucleic acids each encoding an acetyl-CoA pathway enzyme.
 122. The non-naturally occurring microbial organism of claim 120, wherein said microbial organism comprises exogenous nucleic acids encoding each of the acetyl-CoA pathway enzymes of at least one of the pathways selected from (1)-(36).
 123. The non-naturally occurring microbial organism of claim 95, further comprising one or more gene disruptions, said one or more gene disruptions occurring in endogenous genes encoding proteins or enzymes involved in: native production of ethanol, glycerol, acetate, formate, lactate, CO₂, fatty acids, or malonyl-CoA by said microbial organism; transfer of pathway intermediates to cellular compartments other than the cytosol; or native degradation of a FAACPE cycle intermediate or a termination pathway intermediate by said microbial organism, wherein said one or more gene disruptions confer increased production of the compound of Formula (I) in said microbial organism.
 124. The non-naturally occurring microbial organism of claim 123, wherein said protein or enzyme is selected from the group consisting of a fatty acid synthase, an acetyl-CoA carboxylase, a biotin:apoenzyme ligase, an acyl carrier protein, a thioesterase, an acyltransferase, an ACP malonyltransferase, a fatty acid elongase, an acyl-CoA synthetase, an acyl-CoA transferase, an acyl-CoA hydrolase, a pyruvate decarboxylase, a lactate dehydrogenase, an alcohol dehydrogenase, an acid-forming aldehyde dehydrogenases, an acetate kinase, a phosphotransacetylase, a pyruvate oxidase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate phosphatase, a mitochondrial pyruvate carrier, a peroxisomal fatty acid transporter, a peroxisomal acyl-CoA transporter, a peroxisomal carnitine/acylcarnitine transferase, an acyl-CoA oxidase, and an acyl-CoA binding protein.
 125. The non-naturally occurring microbial organism of claim 95, wherein one or more enzymes of the FAACPE cycle or the termination pathway preferentially react with an NADH cofactor or have reduced preference for reacting with an NAD(P)H cofactor, wherein said one or more enzymes of the FAACPE cycle are a 3-ketoacyl-ACP reductase or an enoyl-ACP reductase, and wherein said one or more enzymes of the termination pathway are selected from an acyl-CoA reductase (aldehyde forming), an alcohol dehydrogenase, an acyl-CoA reductase (alcohol forming), a fatty acyl-ACP reductase, and a carboxylic acid reductase.
 126. The non-naturally occurring microbial organism of claim 95, further comprising one or more gene disruptions, said one or more gene disruptions occurring in genes encoding proteins or enzymes that result in an increased ratio of NAD(P)H to NAD(P) present in the cytosol of said microbial organism following said disruptions.
 127. The non-naturally occurring microbial organism of claim 126, wherein said gene encoding a protein or enzyme that results in an increased ratio of NAD(P)H to NAD(P) present in the cytosol of said microbial organism following said disruptions is selected from the group consisting of an NADH dehydrogenase, a cytochrome oxidase, a glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate phosphatase, an alcohol dehydrogenase, a pyruvate decarboxylase, an aldehyde dehydrogenase (acid forming), a lactate dehydrogenase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate:quinone oxidoreductase, a malic enzyme and a malate dehydrogenase.
 128. The non-naturally occurring organism of claim 123 or 126, wherein said one or more gene disruptions comprises a deletion of said one or more genes.
 129. The non-naturally occurring microbial organism of claim 95, wherein said microbial organism is Crabtree positive and is in culture medium comprising excess glucose, thereby increasing the ratio of NAD(P)H to NAD(P) present in the cytosol of said microbial organism.
 130. The non-naturally occurring microbial organism of claim 95, further comprising at least one exogenous nucleic acid encoding an extracellular transporter or an extracellular transport system for the compound of Formula (I).
 131. The non-naturally occurring microbial organism of claim 95, wherein one or more endogenous enzymes involved in: native production of ethanol, glycerol, acetate, formate, lactate, CO2, fatty acids, or malonyl-CoA by said microbial organism; transfer of pathway intermediates to cellular compartments other than the cytosol; or native degradation of a FAACPE cycle intermediate or a termination pathway intermediate by said microbial organism, has attenuated enzyme activity or expression levels.
 132. The non-naturally occurring microbial organism of claim 131, wherein said enzyme is selected from the group consisting of a fatty acid synthase, an acetyl-CoA carboxylase, a biotin:apoenzyme ligase, a thioesterase, an acyl carrier protein, a thioesterase, an acyltransferase, an ACP malonyltransferase, a fatty acid elongase, an acyl-CoA synthetase, an acyl-CoA transferase, an acyl-CoA hydrolase, a pyruvate decarboxylase, a lactate dehydrogenase, a short-chain alcohol dehydrogenase, an acid-forming aldehyde dehydrogenase, an acetate kinase, a phosphotransacetylase, a pyruvate oxidase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate phosphatase, a mitochondrial pyruvate carrier, a peroxisomal fatty acid transporter, a peroxisomal acyl-CoA transporter, a peroxisomal carnitine/acylcarnitine transferase, an acyl-CoA oxidase, and an acyl-CoA binding protein.
 133. The non-naturally occurring microbial organism of claim 95, wherein one or more endogenous enzymes involved in the oxidation of NAD(P)H or NADH, has attenuated enzyme activity or expression levels.
 134. The non-naturally occurring microbial organism of claim 133, wherein said one or more endogenous enzymes are selected from the group consisting of an NADH dehydrogenase, a cytochrome oxidase, a glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate phosphatase, an alcohol dehydrogenase, a pyruvate decarboxylase, an aldehyde dehydrogenase (acid forming), a lactate dehydrogenase, a glycerol-3-phosphate dehydrogenase, a glycerol-3-phosphate:quinone oxidoreductase, a malic enzyme and a malate dehydrogenase.
 135. A method for producing a compound of Formula (I):

wherein R₁ is C₁₋₂₄ linear alkyl; R₂ is CH₂OH, CHO, or COOH; R₃ is H, OH, or oxo (═O); and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four, comprising culturing the non-naturally occurring microbial organism of any one of claims 95-134 under conditions for a sufficient period of time to produce said compound of Formula (I).
 136. The method of claim 135, wherein said method further comprises separating the compound of Formula (I) from other components in the culture.
 137. The method of claim 136, wherein the separating comprises extraction, continuous liquid-liquid extraction, pervaporation, membrane filtration, membrane separation, reverse osmosis, electrodialysis, distillation, crystallization, centrifugation, extractive filtration, ion exchange chromatography, absorption chromatography, or ultrafiltration.
 138. Culture medium comprising bioderived compound of Formula (I):

wherein R₁ is C₁₋₂₄ linear alkyl; R₂ is CH₂OH, CHO, or COOH; R₃ is H, OH, or oxo (═O); and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four, wherein said culture medium is separated from a non-naturally occurring microbial organism of any one of claims 95-134.
 139. A bioderived compound of Formula (I):

wherein R₁ is C₁₋₂₄ linear alkyl; R₂ is CH₂OH, CHO, or COOH; R₃ is H, OH, or oxo (═O); and

represents a single or double bond with the proviso that the valency of the carbon atom to which R₃ is attached is four, wherein said bioderived compound is produced according to the method of any one of claims 135-137.
 140. The bioderived compound of claim 139, wherein said bioderived compound has an Fm value of at least 80%, at least 85%, at least 90%, at least 95% or at least 98%.
 141. A composition comprising said bioderived compound of claim 139 or 140 and a compound other than said bioderived compound.
 142. The composition of claim 141, wherein said compound other than said bioderived compound is a trace amount of a cellular portion of a non-naturally occurring microbial organism having: (i) a formaldehyde fixation pathway; (ii) a formate assimilation pathway; and/or (iii) a methanol metabolic pathway, and a fatty acyl-ACP elongation (FAACPE) cycle in combination with a termination pathway.
 143. A composition comprising the bioderived compound of claim 139 or 140, or a cell lysate or culture supernatant thereof.
 144. A biobased product comprising said bioderived compound of claim 139 or 140, wherein said biobased product is a biofuel, chemical, polymer, surfactant, soap, detergent, shampoo, lubricating oil additive, fragrance, flavor material or acrylate.
 145. The biobased product of claim 144 comprising at least 5%, at least 10%, at least 20%, at least 30%, at least 40% or at least 50% said bioderived compound.
 146. The biobased product of claim 144 or 145, wherein said biobased product comprises a portion of said bioderived compound as a repeating unit.
 147. A molded product obtained by molding a biobased product of any one of claims 144-146, wherein said biobased product is a polymer.
 148. A process for producing a biobased product of any one of claims 144-146 comprising chemically reacting said bioderived compound with itself or another compound in a reaction that produces said biobased product.
 149. The non-naturally occurring microbial organism of any one of claims 1-45 or any one of claims 60-80 or any one of claims 95-134, wherein said acetyl-CoA pathway comprises 1T and 1V.
 150. The non-naturally occurring microbial organism of claim 149, wherein said formaldehyde fixation pathway comprises 1D and 1Z.
 151. The non-naturally occurring microbial organism of claim 149 or 150, wherein said formaldehyde fixation pathway comprises 1B and 1C.
 152. The non-naturally occurring microbial organism of any one of claims 1-45, wherein one or more enzymes of the MI-FAE cycle and/or MD-FAE cycle are each selective for a compound of Formula (II) wherein R₁ is C₉ linear alkyl, C₁₀ linear alkyl, C₁₁, linear alkyl, C₁₂ linear alkyl or C₁₃ linear alkyl.
 153. The non-naturally occurring microbial organism of any one of claims 1-45, wherein one or more enzymes of the termination pathway are each selective for a compound of Formula (II) wherein R₁ is C₉ linear alkyl, C₁₀ linear alkyl, C₁₁, linear alkyl, C₁₂ linear alkyl or C₁₃ linear alkyl.
 154. The non-naturally occurring microbial organism of any one of claims 95-134, wherein one or more enzymes of the FAACPE cycle are each selective for a compound of Formula (II) wherein R₁ is C₉ linear alkyl, C₁₀ linear alkyl, C₁₁, linear alkyl, C₁₂ linear alkyl or C₁₃ linear alkyl.
 155. The non-naturally occurring microbial organism of any one of claims 95-134, wherein one or more enzymes of the termination pathway are each selective for a compound of Formula (II) wherein R₁ is C₉ linear alkyl, C₁₀ linear alkyl, C₁₁, linear alkyl, C₁₂ linear alkyl or C₁₃ linear alkyl.
 156. The non-naturally occurring microbial organism of any one of claims 1-45 or any one of claims 60-80 or any one of claims 95-134, wherein said microbial organism further comprises attenuation of one or more endogenous enzymes selected from DHA kinase, methanol oxidase, PQQ-dependent methanol dehydrogenase, DHA synthase or any combination thereof.
 157. The non-naturally occurring microbial organism of claim 156, wherein said one or more endogenous enzymes is DHA kinase.
 158. The non-naturally occurring microbial organism of claim 156, wherein said one or more endogenous enzymes is methanol oxidase.
 159. The non-naturally occurring microbial organism of claim 156, wherein said one or more endogenous enzymes is PQQ-dependent methanol dehydrogenase.
 160. The non-naturally occurring microbial organism of claim 156, wherein said one or more endogenous enzymes is DHA synthase.
 161. The non-naturally occurring microbial organism of any one of claims 1-45 or any one of claims 60-80 or any one of claims 95-134, wherein said microbial organism further comprises attenuation of one or more endogenous enzymes of a competing formaldehyde assimilation or dissimilation pathway.
 162. The non-naturally occurring microbial organism of any one of claims 1-45 or any one of claims 60-80 or any one of claims 95-134, wherein said microbial organism further comprises a gene disruption of one or more endogenous nucleic acids encoding enzymes selected from DHA kinase, methanol oxidase, PQQ-dependent methanol dehydrogenase, DHA synthase or any combination thereof.
 163. The non-naturally occurring microbial organism of claim 162, wherein said gene disruption is of an endogenous nucleic acid encoding the enzyme DHA kinase.
 164. The non-naturally occurring microbial organism of claim 162, wherein said gene disruption is of an endogenous nucleic acid encoding the enzyme methanol oxidase.
 165. The non-naturally occurring microbial organism of claim 162, wherein said gene disruption is of an endogenous nucleic acid encoding the enzyme PQQ-dependent methanol dehydrogenase.
 166. The non-naturally occurring microbial organism of claim 162, wherein said gene disruption is of an endogenous nucleic acid encoding the enzyme DHA synthase.
 167. The non-naturally occurring microbial organism of any one of claims 1-45 or any one of claims 60-80 or any one of claims 95-134, wherein said microbial organism further comprises a gene disruption of one or more endogenous nucleic acids encoding enzymes of a competing formaldehyde assimilation or dissimilation pathway.
 168. A non-naturally occurring microbial organism having an acetyl-CoA pathway, wherein said acetyl-CoA pathway comprises a pathway selected from: (1) 1T and 1V; (2) 1T, 1W, and 1X; (3) 1U and 1V; (4) 1U, 1W, and 1X; wherein 1T is a fructose-6-phosphate phosphoketolase, wherein 1U is a xylulose-5-phosphate phosphoketolase, wherein 1V is a phosphotransacetylase, wherein 1W is an acetate kinase, wherein 1X is an acetyl-CoA transferase, an acetyl-CoA synthetase, or an acetyl-CoA ligase, wherein said non-naturally occurring microbial organism further comprises a pathway capable of producing isopropanol and an exogenous nucleic acid encoding an isopropanol pathway enzyme expressed in a sufficient amount to produce isopropanol, wherein said isopropanol pathway comprises a pathway selected from: (1) 11V, 11W, 11X, and 11Y; or (2) 11T, 11U, 11W, 11X, and 11Y, wherein 11T is an acetyl-CoA carboxylase, wherein 11U is an acetoacetyl-CoA synthase, wherein 11V is an acetyl-CoA:acetyl-CoA acyltransferase, wherein 11W is an acetoacetyl-CoA hydrolase, an acetoacetyl-CoA transferase, an acetoacetyl-CoA ligase, or a phosphotransacetoacetylase/acetoacetate kinase, wherein 11X is an acetoacetate decarboxylase, wherein 11Y is an acetone reductase or isopropanol dehydrogenase. 